Journal of plant physiology最新文献

{"title":"Hydrogen sulfide: A tiny molecule with a big role in stomatal regulation","authors":"Zhuping Jin , Carlos García‐Mata , Yanxi Pei","doi":"10.1016/j.jplph.2025.154539","DOIUrl":"10.1016/j.jplph.2025.154539","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154539"},"PeriodicalIF":4.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The R2R3-MYB transcription factor MdMYB62 negatively regulates the drought and salt tolerance in apple","authors":"Xin-Long Guo ,&nbsp;Xiang Wu ,&nbsp;Hong-Liang Li ,&nbsp;Ran-Xin Liu ,&nbsp;Jian-Ping An ,&nbsp;Chun-Xiang You","doi":"10.1016/j.jplph.2025.154527","DOIUrl":"10.1016/j.jplph.2025.154527","url":null,"abstract":"<div><div>Drought and salt stresses represent significant environmental constraints that severely impair global plant growth and development. While numerous transcription factors regulating drought and salt stress responses have been identified across plant species, their functional mechanisms remain incompletely understood. In this study, we characterized <em>MdMYB62</em>, a MYB transcription factor from apple (<em>Malus domestica</em>), and elucidated its functional role under abiotic stress conditions. Quantitative reverse transcription PCR (qRT-PCR) analysis revealed that <em>MdMYB62</em> expression was significantly modulated under both drought and salt stress conditions. Functional analyses revealed that overexpression of <em>MdMYB62</em> in apple calli led to increased sensitivity to drought and salt stress.Consistent with these findings, ectopic expression of <em>MdMYB62</em> in Arabidopsis resulted in reduced tolerance to these stress, which was associated with elevated accumulation of reactive oxygen species (ROS). These results collectively establish <em>MdMYB62</em> as a negative regulator of plant stress responses and provide new insights into the molecular mechanisms underlying plant adaptation to abiotic stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154527"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanin and chlorophyll accumulation by targeted metabolomic and transcriptomic analysis involved in pigment accumulation during fruit maturation in Liriope spicata","authors":"Jiannan Shi ,&nbsp;Ye Wang ,&nbsp;Xinyu Wang ,&nbsp;Mengkun Han ,&nbsp;Runzhi Li ,&nbsp;Chunxin Yu ,&nbsp;Zhen Peng ,&nbsp;Yuerong Gao ,&nbsp;Ziyan Liu ,&nbsp;Yuanyue Shen ,&nbsp;Liusheng Duan","doi":"10.1016/j.jplph.2025.154529","DOIUrl":"10.1016/j.jplph.2025.154529","url":null,"abstract":"<div><div><em>Liriope spicata</em> is extensively distributed in China, often cultivated under forest or in semi-shade as a traditional Chinese herbal medicine material, and has attracted widespread global interest due to its high ornamental value. To obtain a better understanding of the differential metabolites between pigmented fruit and the color change from light green to black during development and ripening, we used a targeted metabolomic- and transcriptomic-based approach to investigate the anthocyanin and chlorophyll biosynthesis mechanism. A total of 9 cyanidin derivatives, 8 delphinidin derivatives, 5 malvidin derivatives, 9 pelargonidin derivatives, 6 petunidin derivatives, 4 petunidin derivatives, and 8 flavonoid derivatives were identified in <em>L</em>. <em>spicata</em> fruit at five development stages. Transcriptional factors MYB, bZIP, and WRKY were upregulated, which activated the expression of structural genes. The weighted gene correlation network analysis (WGCNA) of anthocyanins, chlorophyll, and associated genes revealed a regulatory system involved in the pigmentation of light green to black <em>L. spicata</em> fruit. Three phenylalanine deaminase genes (<em>PALs</em>) and a stay green gene (<em>SGR</em>) are involved in anthocyanin and chlorophyll biosynthesis, respectively. In the correlation analysis of anthocyanin components, it was suggested that PAL genes were closely related to different anthocyanins accumulated, moreover, the anthocyanin content and PAL activity show a positive correlation at different stages of fruit maturation. These findings provide new insights into molecular mechanisms of anthocyanin and chlorophyll biosynthesis and regulation of pigmentation changes in fruit. Therefore, this will facilitate the breeding of cultivars with high levels of anthocyanin in <em>L. spicata</em> which enhanced ornamental value and research on potential tinctorial and functional raw material.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154529"},"PeriodicalIF":4.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PdSABP2A involved in jasmonic acid biosynthesis regulates Anoplophora glabripennis resistance of Populus deltoides ‘Shalinyang’","authors":"Ziyi Wang ,&nbsp;Chenxing He ,&nbsp;Yunmei Zhang ,&nbsp;Shuiwen Luo ,&nbsp;Jianrong Wei ,&nbsp;Jianfeng Liu","doi":"10.1016/j.jplph.2025.154528","DOIUrl":"10.1016/j.jplph.2025.154528","url":null,"abstract":"<div><div><em>Populus deltoids</em> ‘Shalinyang’ (PdS) is a new poplar variety with insect resistance cultivated in the western region of China. <em>Anoplophora glabripennis</em> (ALB) is an important forestry pest that causes great harm to poplar trees. However, the research on the insect resistance mechanism of PdS is still unclear, so exploring its defense response mechanism against ALB can provide theoretical and technical support for the subsequent cultivation of new varieties of poplar suitable for extensive planting and resistant to biotic stress. This study primarily aims to elucidate the molecular mechanisms underlying PdS's inducible resistance by jasmonic acid (JA). Here, 50 μM JA was applied to 1-year-old PdS plants to verify its anti-insect effect in PdS. It was found that superoxide dismutase (SOD) activity and hydrogen peroxide (H₂O₂) content increased, malondialdehyde (MDA) content decreased, and the feeding area of ALB leaves and the number of stem bite marks decreased by 56.92 % and 49.33 % respectively compared to the control group. The results indicated that JA treatment had a positive regulatory effect on insect resistance in PdS. Based on the transcriptome data, the key gene <em>PdSABP2A</em> was screened from the methylesterase (MES) gene family that played an important role in plant defense response. The expression level of <em>PdSABP2A</em> gene also significantly increased in the external JA treatment experiment. The virus-induced gene silencing (VIGS) experiment was used for gene function validation, and it was found that the expression level of <em>PdSABP2A</em> gene decreased by 73.72 %, and the JA content also significantly decreased. In addition, PdS subjected to the VIGS silencing treatment were more severely nibbled by ALB. The larvae inside PdS plants grew faster, had higher activity of digestive and defense enzymes, and lower mortality rates, all of which indicated a decrease in PdS insect resistance. These findings indicated that the <em>PdSABP2A</em> gene is involved in regulating the synthesis of JA and inducing the defense response of PdS against ALB.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154528"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MbWRKY50 confers cold and drought tolerance through upregulating antioxidant capacity associated with ROS scavenging","authors":"Xinhui Wang ,&nbsp;Yingnan Li ,&nbsp;Zhuo Chen ,&nbsp;Longfeng Li ,&nbsp;Qiqi Li ,&nbsp;Zihan Geng ,&nbsp;Wanda Liu ,&nbsp;Ruining Hou ,&nbsp;Lihua Zhang ,&nbsp;Deguo Han","doi":"10.1016/j.jplph.2025.154526","DOIUrl":"10.1016/j.jplph.2025.154526","url":null,"abstract":"<div><div>Plant growth and development can be impacted by abiotic factors, including low temperature and dryness. Numerous studies have shown that plant responses to stress are largely influenced by the WRKY transcription factors (TFs). However, there are few studies on the role of WRKY genes in the stress response of <em>Malus</em> plants. In this experiment, <em>Malus baccata</em> (L.) Borkh was selected as the material, and the WRKY family gene <em>MbWRKY50</em> was cloned using a gene cloning technique. Phylogenetic tree analysis revealed that MbWRKY50 and MdWRKY50 have the highest homology. Furthermore, a green fluorescent protein (GFP) fusion protein expression assay revealed that the MbWRKY50 protein is located in the nucleus. The results of RT-qPCR showed that the expression of <em>MbWRKY50</em> was increased in the roots and fully grown leaves of <em>M. baccata</em>, and the response to low temperature and drought environment was enhanced<em>.</em> The <em>MbWRKY50</em> gene was transferred into tomato, which could better adapt to the cold and dry living conditions. In contrast to wild-type (WT) and untransformed (UL) tomato lines, overexpression of <em>MbWRKY50</em> boosts the activities of superoxide dismutase (SOD) and peroxidase (POD). Moreover, it leads to a notable reduction in the concentrations of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and superoxide anion free radicals (O₂⁻). The results showed that MbWRKY50 activated the expression levels of <em>LeABI3</em>, <em>LeNCED1</em>, <em>LeABF4</em>, <em>LeDREB1</em>, <em>LeCBF1</em>, and <em>LeCBF3</em> by binding to cold binding factor/dehydration response element (CBF/DREB) or participating in ABA synthesis, thereby enhancing the resistance of transgenic tomatoes to low temperature and drought stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"310 ","pages":"Article 154526"},"PeriodicalIF":4.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin improves aluminum tolerance in soybean through modification of cell wall and vacuolar compartmentalization of aluminum","authors":"Huanan Li ,&nbsp;Ziyu Jia ,&nbsp;Kunxia Cheng ,&nbsp;Luyu Wang ,&nbsp;Junjun Huang ,&nbsp;Huahua Wang","doi":"10.1016/j.jplph.2025.154525","DOIUrl":"10.1016/j.jplph.2025.154525","url":null,"abstract":"<div><div>Aluminum (Al) toxicity has a significant adverse impact on plant growth and crop yield. Melatonin (MT) is involved in plant responses to various environmental stresses. However, the role of MT in mitigating Al toxicity remains largely unknown in soybean (<em>Glycine</em> max L.). In this study, the findings indicate that MT application alleviates Al-induced root growth inhibition and reduces Al accumulation in the cell wall. MT application under Al stress decreased the pectin and hemicellulose 1 content in the root tip cell wall and increased pectin methyl esterification, leading to reduced Al binding in the cell wall fractions. Additionally, MT treatment under Al stress inhibited lignin synthesis in the root tip cell wall, thereby alleviating the increased rigidity of cell wall and promoting its expansion. Furthermore, MT application under Al stress modulated the expression of Al transport-related genes (<em>GmCDT3</em>, <em>GmNrat1</em>, <em>GmIREG3</em> and <em>GmALS1</em>), reducing cytoplasmic Al accumulation and enhancing vacuolar Al sequestration. Taken together, these findings suggest that MT mitigates Al toxicity in soybean by reducing Al deposition in the cell wall and enhancing Al sequestration in the vacuole. This study offers clues for enhancing crop resistance to Al toxicity in acidic soils.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"310 ","pages":"Article 154525"},"PeriodicalIF":4.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-Aminolevulinic Acid: from pyrrole biosynthetic precursor to multifunctional plant growth regulator","authors":"Liangju Wang,&nbsp;Jianting Zhang,&nbsp;Qingze Zhao,&nbsp;Liuzi Zhang","doi":"10.1016/j.jplph.2025.154524","DOIUrl":"10.1016/j.jplph.2025.154524","url":null,"abstract":"<div><div>5-Aminolevulinic acid (ALA) is a non-protein δ-amino acid and an essential precursor of tetrapyrrole compound biosynthesis. Nowadays, it is a well-known natural plant growth regulator with multiple biological regulatory functions. In this review, we summarize the regulatory effects of ALA in promoting plant growth and the development of organs such as roots, stems, leaves, flowers, and fruits under normal conditions as well as stressful conditions. We emphasize the newly revealed signaling transduction and transcriptional regulatory mechanisms of ALA in maintaining root functions against abiotic stresses, improving leaf photosynthetic performance, and enhancing fruit appearance and flavor qualities as well as storage. Although most of the current reports on ALA are still apparent effect descriptions rather than mechanism explorations, studies suggest that ALA can facilitate agricultural development toward higher yield, quality, efficiency, and safety. The regulatory mechanisms of ALA at different levels need further study in the future.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"310 ","pages":"Article 154524"},"PeriodicalIF":4.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling regional and altitudinal lipidomic analyte signatures of the argan tree (Argania spinosa L.) for environmental adaptation","authors":"El Faqer Abdelmoiz ,&nbsp;Rabeh Karim ,&nbsp;Rachidi Farid ,&nbsp;Assemar Fatima ezzahra ,&nbsp;Aasfar Abderrahim ,&nbsp;Filali-Maltouf Abdelkarim ,&nbsp;Belkadi Bouchra","doi":"10.1016/j.jplph.2025.154523","DOIUrl":"10.1016/j.jplph.2025.154523","url":null,"abstract":"<div><div>Environmental factors such as altitude, precipitation, and temperature shape the lipidomic profiles of the argan tree (<em>Argania spinosa</em> L.), supporting its adaptation to stress. This study investigated lipidomic profiling and pathways in argan tree leaves from four altitudinal zones (A: low, B: moderate, C: high, D: very high) across three Moroccan regions (Chtouka Aït Baha, Essaouira, and Tiznit) using Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS workflow included a transmethylation step that cleaves ester bonds and acetylations, yielding analytes derived from diverse precursor lipids such as glycerolipids, sterol esters, and wax esters. We identified 139 lipid analytes, categorized into fatty acyls (53 %), prenol lipids (41 %), and steroids (6 %). Shared lipids across all zones highlight core metabolic pathways essential for resilience, while unique lipids reflect zone-specific adaptations. Fourteen known analytes were identified as critical markers for regional adaptations through multivariate analyses, including Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA), and Variable Importance in Projection (VIP) scores. Among these, three analytes (methyl 18-methyleicosanoate, Z,Z-11,13-Hexadecadien-1-ol, and 11-Octadecenoic acid) showed the highest accumulation in Zone A, whereas eleven analytes (Henicosyl formate, Dodecyl 2-methylbutanoate, Methyl 21-methyl-hexacosanoate, Methyl 13-methyltetradecanoate, Cetoleic acid, (Urs-12-en-3-ol, acetate, (3.beta.)-), Medicagenic acid, 2-(4a,8-Dimethyl-6-oxo-1,2,3,4,4a,5,6,8a-octahydro-naphthalen-2-yl)-propionaldehyde, A′-Neogammacer-22(29)-en-3-one, Pregna-5,17(20)-dien-3-ol, (3.beta.,17E)-, and estra-1,3,5(10)-trien-17-one, 3,4-bis(acetyloxy)- exhibited significant increases in Zone D. Multiple Linear Regression analysis showed that precipitation positively influenced analyte concentration (p = 0.00033), while altitude had a significant negative effect (p = 0.039). Pathways analysis highlighted the roles of cutin, suberin, and wax biosynthesis, as well as linoleic acid metabolism, in altitude-driven adaptations. This study demonstrates the metabolic plasticity of <em>Argania spinosa</em> L., offering insights for its conservation amidst climate change.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154523"},"PeriodicalIF":4.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next generation technologies for protein structure determination: challenges and breakthroughs in plant biology applications","authors":"Veronica G. Maurino","doi":"10.1016/j.jplph.2025.154522","DOIUrl":"10.1016/j.jplph.2025.154522","url":null,"abstract":"<div><div>Advancements in structural biology have significantly deepened our understanding of plant proteins, which are central to critical biological functions such as photosynthesis, metabolism, signal transduction, and structural architechture. Gaining insights into their structures is crucial for unraveling their functions and mechanisms, which in turn has profound implications for agriculture, biotechnology, and environmental sustainability. Traditional methods in protein structural biology often fall short in addressing large protein assemblies and membrane proteins, and, in particular the dynamics and structural features of proteins in the native cellular context. This paper explores how next-generation technologies are transforming the field of plant protein structural biology, offering powerful tools to overcome longstanding obstacles and enabling remarkable scientific breakthroughs. Key technologies discussed include advanced X-ray crystallography, Cryo-Electron microscopy, Nuclear Magnetic Resonance spectroscopy, Cross-linking mass spectrometry, and Artificial Intelligence-driven approaches. These technologies are examined in terms of their challenges, innovations, and application with particular emphasis on their relevance to plant systems. Future directions in plant protein structural biology are also discussed. Although technical details are not covered in depth, readers are referred to the primary literature for more comprehensive information.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"310 ","pages":"Article 154522"},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Agrobacterium-mediated genetic transformation and CRISPR/Cas9-guided gene editing in Elymus nutans","authors":"Cheng Li ,&nbsp;Xiaomei Peng ,&nbsp;Zhengshe Zhang ,&nbsp;Yaling Liu ,&nbsp;García-Caparrós Pedro ,&nbsp;Chunxiang Fu ,&nbsp;Yongping Yang ,&nbsp;Quanmin Dong ,&nbsp;Yuanwen Duan ,&nbsp;Xudong Sun","doi":"10.1016/j.jplph.2025.154513","DOIUrl":"10.1016/j.jplph.2025.154513","url":null,"abstract":"<div><div><em>Elymus nutans</em>, an allohexaploid (2n = 6x = 42) species with a StStHHYY genome, is a native perennial in the alpine grasslands of the Qinghai-Xizang Plateau, and has been widely used for artificial pasture and ecological restoration as a forage grass with highest yield on the plateau. Nevertheless, the lack of a stable transformation system has impeded further efforts to trait improvement of <em>E. nutans</em>. In the present study, we established a reliable <em>Agrobacterium</em>-mediated genetic transformation system for <em>E. nutans</em>, and successfully generated <em>EnTCP4</em>-edited plants using the CRISPR/Cas9 system. The editing efficiency achieved 19.23 % in <em>E. nutans</em>. Knocking out <em>EnTCP4</em> significantly delayed flowering and enhanced water-deficit stress resistance. This research represents a significant breakthrough in the genetic transformation and gene editing of <em>E. nutans</em>, laying a technological foundation to gain insight into gene functions and molecular breeding in <em>E. nutans</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"310 ","pages":"Article 154513"},"PeriodicalIF":4.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}