ZmThr1, a chloroplast-targeted regulator from maize, enhances growth and grain development but reprograms amino acid and starch metabolism in Arabidopsis

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2025-09-19 DOI:10.1016/j.jplph.2025.154613

Zongjie Wang , Peng Jiao , Yanjun Dong , Xinrui Ma , Manyao Liu , Jianhua Zhang , Shuyan Guan , Yiyong Ma

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引用次数: 0

Abstract

The deficiency of essential amino acids in maize, a vital food and cash crop worldwide that is crucial for both humans and livestock, poses a significant challenge for high-quality agricultural and economic development. In this study, we investigated the role of ZmThr1 in the regulation of seed amino acid accumulation in Colombian wild-type (WT) Arabidopsis. Our experimental results indicated that ZmThr1 encodes a 57.6 kDa protein localized in plant chloroplasts. Compared with Columbia WT Arabidopsis, overexpression of this gene promoted seedling growth and seed development in Arabidopsis. However, the gene negatively regulated the amino acid content in Arabidopsis seeds, which decreased by 17.48 % relative to that in WT Arabidopsis. In addition, the starch content in the seeds was reduced by 52.8 % compared with that in WT Arabidopsis. In conclusion, overexpression of ZmThr1 negatively regulated both amino acid and starch contents in Arabidopsis seeds.

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ZmThr1是一种来自玉米叶绿体的调控因子，可促进拟南芥的生长和籽粒发育，但对氨基酸和淀粉代谢进行重编程。

玉米是世界范围内重要的粮食和经济作物，对人类和牲畜都至关重要，玉米缺乏必需氨基酸对高质量农业和经济发展构成了重大挑战。在这项研究中，我们研究了ZmThr1在哥伦比亚野生型（WT）拟南芥种子氨基酸积累中的调控作用。我们的实验结果表明，ZmThr1在植物叶绿体中编码一个57.6 kDa的蛋白。与哥伦比亚WT拟南芥相比，该基因的过表达促进了拟南芥幼苗的生长和种子的发育。然而，该基因负调控拟南芥种子中氨基酸含量，与WT拟南芥相比，氨基酸含量降低了17.48%。此外，与WT拟南芥相比，种子中的淀粉含量降低了52.8%。综上所述，ZmThr1过表达对拟南芥种子中氨基酸和淀粉含量均有负调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.