ABA和GA信号平衡的改变介导了小麦种子成熟过程中诱导和保持休眠的遗传变异

IF 4 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2024-11-28 DOI:10.1016/j.jplph.2024.154394

Tran-Nguyen Nguyen, Pham Anh Tuan, Deepak Sharma, Belay T. Ayele

{"title":"ABA和GA信号平衡的改变介导了小麦种子成熟过程中诱导和保持休眠的遗传变异","authors":"Tran-Nguyen Nguyen, Pham Anh Tuan, Deepak Sharma, Belay T. Ayele","doi":"10.1016/j.jplph.2024.154394","DOIUrl":null,"url":null,"abstract":"<div><div>Induction and retention of dormancy are among the physiological processes that take place during seed maturation; however, the molecular mechanisms underlying these events are poorly understood in wheat. This study revealed that seed maturation in wheat is associated with decreases in abscisic acid (ABA) and gibberellin (GA) levels irrespective of dormancy level exhibited by the seeds mainly via expression of specific ABA (<em>TaCYP707A</em>1) and GA (<em>TaGA3ox2</em>, <em>TaGA2ox3</em> and <em>TaGA2ox6</em>) metabolism genes. Consistently, ABA to GA level ratio decreased during maturation in both highly dormant and low-dormant seeds with no apparent difference in the ratio of their levels between the two seed samples. Our data, however, showed a close association between the induction and retention of dormancy during seed maturation and modulation of the balance between ABA and GA signaling via expression of specific genes that acts as positive regulators seed response to ABA (<em>TaPYL5</em> and <em>TaABI5</em>) and GA (<em>TaGAMyb</em>). Consistently, the highly dormant and low-dormant seeds exhibited substantial variation in their sensitivity to ABA and GA during their maturation. The findings of this study highlight that genetic variation in induction and retention of dormancy during wheat seed maturation can be mediated by a shift in balance between seed sensitivity to ABA and GA independent of a shift in balance between their levels.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154394"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alteration in the balance between ABA and GA signaling mediates genetic variation in induction and retention of dormancy during seed maturation in wheat\",\"authors\":\"Tran-Nguyen Nguyen, Pham Anh Tuan, Deepak Sharma, Belay T. Ayele\",\"doi\":\"10.1016/j.jplph.2024.154394\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Induction and retention of dormancy are among the physiological processes that take place during seed maturation; however, the molecular mechanisms underlying these events are poorly understood in wheat. This study revealed that seed maturation in wheat is associated with decreases in abscisic acid (ABA) and gibberellin (GA) levels irrespective of dormancy level exhibited by the seeds mainly via expression of specific ABA (<em>TaCYP707A</em>1) and GA (<em>TaGA3ox2</em>, <em>TaGA2ox3</em> and <em>TaGA2ox6</em>) metabolism genes. Consistently, ABA to GA level ratio decreased during maturation in both highly dormant and low-dormant seeds with no apparent difference in the ratio of their levels between the two seed samples. Our data, however, showed a close association between the induction and retention of dormancy during seed maturation and modulation of the balance between ABA and GA signaling via expression of specific genes that acts as positive regulators seed response to ABA (<em>TaPYL5</em> and <em>TaABI5</em>) and GA (<em>TaGAMyb</em>). Consistently, the highly dormant and low-dormant seeds exhibited substantial variation in their sensitivity to ABA and GA during their maturation. The findings of this study highlight that genetic variation in induction and retention of dormancy during wheat seed maturation can be mediated by a shift in balance between seed sensitivity to ABA and GA independent of a shift in balance between their levels.</div></div>\",\"PeriodicalId\":16808,\"journal\":{\"name\":\"Journal of plant physiology\",\"volume\":\"304 \",\"pages\":\"Article 154394\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of plant physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0176161724002256\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724002256","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

诱导和保持休眠是种子成熟过程中发生的生理过程之一；然而，这些事件背后的分子机制在小麦中知之甚少。本研究表明，小麦种子成熟与ABA和赤霉素水平的降低有关，而与种子的休眠水平无关，主要是通过ABA （TaCYP707A1）和GA （TaGA3ox2、TaGA2ox3和TaGA2ox6）代谢基因的表达。ABA / GA水平比值在高度休眠和低休眠种子成熟过程中均呈下降趋势，两种种子样品间ABA / GA水平比值无明显差异。然而，我们的数据显示，种子成熟过程中休眠的诱导和保持与ABA和GA信号平衡的调节之间存在密切联系，通过表达作为ABA （TaPYL5和TaABI5）和GA （TaGAMyb）正调节因子的特定基因，种子对ABA的反应。同样，高度休眠和低休眠种子在成熟过程中对ABA和GA的敏感性也有很大差异。本研究结果强调，小麦种子成熟过程中诱导和保持休眠的遗传变异可能是由种子对ABA和GA敏感性之间的平衡变化介导的，而不依赖于ABA和GA水平之间的平衡变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Alteration in the balance between ABA and GA signaling mediates genetic variation in induction and retention of dormancy during seed maturation in wheat

Induction and retention of dormancy are among the physiological processes that take place during seed maturation; however, the molecular mechanisms underlying these events are poorly understood in wheat. This study revealed that seed maturation in wheat is associated with decreases in abscisic acid (ABA) and gibberellin (GA) levels irrespective of dormancy level exhibited by the seeds mainly via expression of specific ABA (TaCYP707A1) and GA (TaGA3ox2, TaGA2ox3 and TaGA2ox6) metabolism genes. Consistently, ABA to GA level ratio decreased during maturation in both highly dormant and low-dormant seeds with no apparent difference in the ratio of their levels between the two seed samples. Our data, however, showed a close association between the induction and retention of dormancy during seed maturation and modulation of the balance between ABA and GA signaling via expression of specific genes that acts as positive regulators seed response to ABA (TaPYL5 and TaABI5) and GA (TaGAMyb). Consistently, the highly dormant and low-dormant seeds exhibited substantial variation in their sensitivity to ABA and GA during their maturation. The findings of this study highlight that genetic variation in induction and retention of dormancy during wheat seed maturation can be mediated by a shift in balance between seed sensitivity to ABA and GA independent of a shift in balance between their levels.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.