Journal of plant physiology最新文献

{"title":"Overexpression of pumpkin (Cucurbita pepo L.) CpVQ20 increases resistance to powdery mildew via antioxidant defense and callose accumulation","authors":"Shiheng Li,&nbsp;Ping Wang","doi":"10.1016/j.jplph.2025.154578","DOIUrl":"10.1016/j.jplph.2025.154578","url":null,"abstract":"<div><div><em>Cucurbita pepo</em> powdery mildew (PM) is mainly caused by <em>Podosphaera xanthii</em>. It can readily induce wilting of pumpkin (<em>Cucurbita pepo</em> L.) branches and leaves, and may even lead to stunted growth and fruit deformities, significantly impacting both the quality and yield of <em>Cucurbita pepo</em>. VQ (Valine-glutamine) proteins play a crucial role in enhancing plant resistance to various abiotic and biotic stresses. In this study, qPCR analysis showed that after powdery mildew infection, the relative expression of <em>CpVQ20</em> increased in disease-resistant material F2 and decreased in susceptible material M3. <em>Cucurbita pepo CpVQ20</em> was localized in the nucleus. We observed a reduced incidence of powdery mildew in <em>CpVQ20</em>-overexpression (OE) plants compared to the wild-type (WT). Furthermore, powdery mildew mycelium grew slower and accumulated less. The activities of antioxidases were enhanced, while the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) were diminished. Overexpression of <em>CpVQ20</em> increased callose content in <em>Cucurbita pepo</em>. The expression levels of callose synthase gene and defense-associated genes were elevated. This study concludes that <em>CpVQ20</em> positively regulates resistance to powdery mildew. This pioneering research establishes a robust foundation for future breakthroughs in gene improvement and genetic breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154578"},"PeriodicalIF":4.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin as a multifaceted stress protector in rice: Mechanisms, synergies, and knowledge gaps","authors":"Saiqa Menhas ,&nbsp;Daohui Lin ,&nbsp;Saiyong Zhu ,&nbsp;Sikandar Hayat ,&nbsp;Tariq Aftab ,&nbsp;Weiping Liu ,&nbsp;Kashif Hayat","doi":"10.1016/j.jplph.2025.154577","DOIUrl":"10.1016/j.jplph.2025.154577","url":null,"abstract":"<div><div>Rice productivity, a cornerstone of global food security, is increasingly threatened by a spectrum of abiotic and biotic stressors, including heavy metal toxicity, salinity, drought, temperature extremes, flooding/water logging, nutrient deficiencies, and pathogens or pest infestations. Melatonin (N-acetyl-5-methoxytryptamine), also referred to as phytomelatonin, was first identified in plants in 1995 and has since emerged as a potent antioxidant and versatile signaling molecule. It plays a critical role in integrating hormonal networks and modulating stress responses in plants, including rice. Both endogenous and exogenously applied melatonin enhance rice tolerance to multiple stress conditions by improving photosynthetic efficiency, reinforcing antioxidant defense systems, maintaining ionic and osmotic homeostasis, and regulating growth and development processes. In the context of biotic stress, melatonin activates innate immune mechanisms, including modulation of defense genes, synthesis of phytoalexins, and fortification of structural barriers, thereby enhancing resistance to pathogens and insect herbivores. Notably, combinatorial applications of melatonin with silicon and nano-zero-valent iron have demonstrated synergistic effects, significantly augmenting stress resilience under complex environmental conditions. Despite these advancements, key knowledge gaps persist regarding mechanistic understanding of melatonin signaling, particularly its interaction with the <em>OsPMTR1</em> receptor, as well as its efficacy under multi-stress field scenarios. Moreover, melatonin's functional outcomes are influenced by rice genotype and environmental context, underscoring the need for optimized application strategies (such as foliar spray, seed priming, and root drenching), and precise dosage calibration to maximize protective benefits while avoiding phytotoxic effects. This review synthesizes current insights into melatonin biosynthesis, signaling pathways, and its multifaceted roles in rice stress physiology, while identifying critical knowledge gaps and underscoring its potential as an integrative and sustainable strategy for advancing climate-resilient rice production.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154577"},"PeriodicalIF":4.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of CtWRKY70 transcription factor from Cynanchum thesioides in salt and drought stress resistance","authors":"Xiaoyao Chang ,&nbsp;Xiaoyan Zhang ,&nbsp;Xiumei Huang ,&nbsp;Fenglan Zhang ,&nbsp;Zhongren Yang","doi":"10.1016/j.jplph.2025.154575","DOIUrl":"10.1016/j.jplph.2025.154575","url":null,"abstract":"<div><div>The <em>WRKY</em> transcription factor <em>CtWRKY70</em> from <em>Cynanchum thesioides</em> was functionally characterized to explore its role in abiotic stress responses. <em>CtWRKY70</em>, encoding a 340-amino acid protein from the WRKY Group III subfamily, localizes to the nucleus and exhibits transcriptional activation activity. Its expression is significantly induced by salt and drought stress. Overexpression of <em>CtWRKY70</em> in <em>Arabidopsis</em> improved tolerance to both stresses, as evidenced by enhanced survival rates, maintained biomass, and preserved chlorophyll content. Transgenic lines exhibited elevated antioxidant enzyme activities (SOD, CAT, POD) and increased proline accumulation, with <em>CtWRKY70</em> directly bound to the promoter of the <em>AtSOD1</em> gene as confirmed by electrophoretic mobility shift assay (EMSA) and yeast one-hybrid (Y1H) assays, indicating enhanced ROS scavenging and osmoregulation. In contrast, CtWRKY70-silenced plants showed heightened stress sensitivity, characterized by greater wilting, increased stomatal aperture, and accelerated water loss. Y2H and BiFC assays confirmed the interaction of CtWRKY70 with another stress-responsive WRKY protein, CtWRKY41. These results demonstrate that <em>CtWRKY70</em> positively regulates drought and salt tolerance by coordinating antioxidant defense and osmotic adjustment. This study provides valuable insights into the molecular mechanisms of WRKY-mediated stress adaptation in horticultural species, positioning <em>CtWRKY70</em> as a potential genetic target for improving crop resilience.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154575"},"PeriodicalIF":4.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sugar transporter GmSWEET48 impacts seed yield and composition of soybean","authors":"Lin Zhu,&nbsp;Feng Lin,&nbsp;Gangao Zhang,&nbsp;Jingya Yuan,&nbsp;Like Shen,&nbsp;Qun Zhang,&nbsp;Wenhua Zhang","doi":"10.1016/j.jplph.2025.154576","DOIUrl":"10.1016/j.jplph.2025.154576","url":null,"abstract":"<div><div>During the early stages of seed development, the small embryo receives large amounts of sugar from the liquid endosperm of the developing seed. A sugar deficit can lead to severe seed abortion and yield loss. However, the key factors influencing sugar transport and crop yield remain largely unknown. In this work, we identified a plasma membrane-localized sugar transporter, <em>GmSWEET48</em>, that was highly expressed in developing soybean seeds. Heterologous expression showed that GmSWEET48 transported fructose and glucose in yeast systems and exhibited ¹⁴C-labeled sucrose influx and efflux activities in <em>Xenopus</em> oocytes. Overexpression of <em>GmSWEET48</em> decreased the levels of sucrose and oil but increased protein levels in seeds and promoted seed yield. In conclusion, GmSWEET48 regulates sugar transport during early seed development and ultimately regulates seed yield and composition.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154576"},"PeriodicalIF":4.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing woody oil biodiesel production in yellowhorn via phenotypic marker development: a study of trait–index associations and germplasm screening","authors":"Wei Zhang ,&nbsp;Linkun Li ,&nbsp;Puxia Wu ,&nbsp;Hui Yang ,&nbsp;Huihui Xu ,&nbsp;Quanxin Bi ,&nbsp;Changchun Shi ,&nbsp;Libing Wang","doi":"10.1016/j.jplph.2025.154574","DOIUrl":"10.1016/j.jplph.2025.154574","url":null,"abstract":"<div><div>The seed oil of yellowhorn (<em>Xanthoceras sorbifolia</em> Bunge) represents a promising feedstock for biodiesel production, but the detection process associated with its quality is both cumbersome and costly. This work focused on early selection of high-quality germplasm to reduce production costs and enhance genetic gains. An analysis of the relationships between 25 quantitative leaf traits and 8 biodiesel characterization indices in yellowhorn at the experimental and demonstration base in Tongliao City, China, identified 5 key leaf functional traits—leaf Soil Plant Analysis Development (SPAD), frond number (FN), leaf rate of water content (LRWC), leaf shape index (LSI), and the wax-to-leaf thickness ratio (W/L)—that showed significant correlations with the biodiesel characterization indices. These traits were identified as critical indicators for predicting biodiesel quality. Furthermore, a predictive map was developed to delineate optimal biodiesel characteristics, encompassing the ranges of SPAD (35.00–49.92), FN (13.17–18.83), LRWC (0.37 %–0.51 %), LSI (0.23–0.40), and W/L (0.01–0.04). The findings of this study provide technical support for employing straightforward and testability traits to forecast complex indicators, thereby facilitating the preliminary selection of high-quality biodiesel yellowhorn germplasm breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154574"},"PeriodicalIF":4.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient overexpression of CpVQ27 reduced the Cucurbita pepo's resistance to powdery mildew","authors":"Shiheng Li,&nbsp;Ping Wang","doi":"10.1016/j.jplph.2025.154573","DOIUrl":"10.1016/j.jplph.2025.154573","url":null,"abstract":"<div><div>Powdery mildew (PM) is the main disease in pumpkin (<em>Cucurbita pepo</em>) cultivation and one of the main factors affecting <em>Cucurbita pepo</em> yield. The VQ (Valine-Glutamine) proteins play a crucial role in plant responses to abiotic stresses such as drought and salinity, and biotic stress from pathogenic bacteria. Transcriptomic and qPCR analysis showed that after powdery mildew infection for 24 and 48 h, the FPKM and relative expression of <em>CpVQ27</em> decreased in disease-resistant material F2 and increased in susceptible material M3. Moreover, the FPKM value and relative expression of <em>CpVQ27</em> in M3 were higher than those in F2. To investigate <em>CpVQ27</em>'s responsiveness to powdery mildew infection, we transiently overexpressed <em>CpVQ27</em> in virus-susceptible variety MRJ. The GFP fluorescence assay, viral disease phenotyping, and qPCR collectively confirmed the successful transient overexpression of <em>CpVQ27</em> in <em>Cucurbita pepo</em>. Compared to the control group, transient overexpression of <em>CpVQ27</em> worsened the powdery mildew disease symptoms. Furthermore, the mycelium of powdery mildew grew faster and accumulated more extensively. There was an increase in ROS and MDA content, along with decreased antioxidant enzyme activities and callose levels. Moreover, the expression levels of defense-related genes were reduced. Transient overexpression of <em>CpVQ27</em> reduced the <em>Cucurbita pepo</em>'s resistance to powdery mildew. Our discoveries lay a solid and comprehensive theoretical foundation for future disease resistance breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154573"},"PeriodicalIF":4.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABA is involved in OsDSR3-mediated regulation of alkali tolerance in rice","authors":"Xuping Lu ,&nbsp;Weifang Min ,&nbsp;Yangmengfei She ,&nbsp;Tinglu Liao ,&nbsp;Donghao Xiao ,&nbsp;Lei Tian ,&nbsp;Peifu Li ,&nbsp;Chengke Luo","doi":"10.1016/j.jplph.2025.154571","DOIUrl":"10.1016/j.jplph.2025.154571","url":null,"abstract":"<div><div>Alkali stress is one of the most damaging abiotic stresses that affect rice growth and yield. The Domain of unknown function (DUF) protein family and abscisic acid (ABA) are critical for abiotic stress tolerance in plants. We previously identified <em>OsDSR3</em>, a novel stress-responsive gene from the DUF966 family that positively regulates rice tolerance to alkali stress. However, it remains unclear whether <em>OsDSR3</em> relies on the ABA signaling to modulate the molecular mechanisms underlying rice alkali resistance. Using RNA sequencing and RT-qPCR, we found differential expression of ABA-related genes in <em>OsDSR3</em> overexpression lines under alkaline stress. Further analysis revealed that <em>OsDSR3</em> overexpression lines exhibited increased sensitivity to ABA treatment, whereas <em>osdsr3</em> mutants exhibited the opposite phenotype. Consistent with these findings, the ABA content was significantly higher in <em>OsDSR3</em> overexpression lines and lower in <em>osdsr3</em> mutants compared to wild type. In addition, the exogenous ABA application enhanced the alkali tolerance of <em>OsDSR3</em> overexpression lines. This enhancement was attributed to the increased activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), elevated levels of osmotic regulating substances (proline, soluble proteins), reduced levels of reactive oxygen species (ROS including superoxide anion and hydrogen peroxide), maintenance of membrane integrity, accumulation of endogenous ABA levels, and activation of gene expression related to the ABA signaling. These effects were significantly more pronounced in <em>OsDSR3</em> overexpression lines than in the <em>osdsr3</em> mutant. Furthermore, using a yeast two-hybrid assay, we demonstrated that OsDSR3 interacts with OsMT-3a. This interaction enhances ROS scavenging via the ABA pathway, thereby positively regulating alkaline tolerance in rice. This study provides deeper insight into the mechanism of <em>OsDSR3</em> regulating alkali stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154571"},"PeriodicalIF":4.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrate sensing and response in Plants: From calcium signaling to phytohormone regulation","authors":"Ling Hao ,&nbsp;Lingdong Meng ,&nbsp;Xiaoping Wang ,&nbsp;Jianjun Qu ,&nbsp;Zhonghao Jiang ,&nbsp;Xin Song","doi":"10.1016/j.jplph.2025.154572","DOIUrl":"10.1016/j.jplph.2025.154572","url":null,"abstract":"<div><div>Plants need to acquire sufficient nitrogen (N) from the soil for their growth and development. Nitrate (NO₃⁻) is the major source of N for plants in aerobic soils. In addition to its role as a nutrient, nitrate also acts as a signaling molecule to reprogram plant metabolism and trigger changes in plant architecture. With the development of genomics technologies and genetic tools, breakthroughs in the understanding of the nitrate signaling network have been made over the past years. In this review, we will discuss the mechanisms of nitrate sensing and its transcriptional response throughout the plant, with an emphasis on the effect of nitrate-elicited calcium signal on the primary nitrate response (PNR). Recent studies have not only identified a second nitrate sensor, NLP7, but also identified calcium-dependent kinases (CPKs) as a molecular link between membrane-localized nitrate receptor NRT1.1 (CHL1/NPF6.3) and NLP transcription factors, which bridges the nitrate gap. We also discuss the latest progress on the interaction between nitrate signal and hormonal pathways for local and systematical developmental responses in the model plant <em>Arabidopsis thaliana</em> roots. A holistic view of how all the identified signals crosstalk to orchestrate the thousands of N responses is the key for the sustainable development of agriculture.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154572"},"PeriodicalIF":4.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sporopollenin-chitosan microspheres loaded with an endophytic fungus Talaromyces neorugulosus R-209 for promoting development and controlling root rot in pigeon pea","authors":"Jin-Xian Fu ,&nbsp;Jiao Jiao ,&nbsp;Qing-Yan Gai ,&nbsp;Yu-Jie Fu ,&nbsp;Mo-Nan Wen ,&nbsp;Xiao-Qing Wang ,&nbsp;Jing He","doi":"10.1016/j.jplph.2025.154569","DOIUrl":"10.1016/j.jplph.2025.154569","url":null,"abstract":"<div><div>Plant-beneficial microbes can be effective as biological agents for promoting development and controlling diseases in plants. However, direct inoculation of non-encapsulated plant-beneficial microbes into the soils can affect their vitality and efficacy. A novel bio-based encapsulant, sporopollenin-chitosan microspheres (SCMs), was developed to load an endophytic fungus <em>Talaromyces neorugulosus</em> R-209 with antagonistic activities against the root rot pathogen (<em>Rhizoctonia solani</em> AG4) and plant growth-promoting functions. The results showed that <em>T. neorugulosus</em> R-209 encapsulated in SCMs (<em>Tn</em>R-209−SCMs) could significantly enhance fungal spore germination rates and available nitrogen/phosphorus levels in the soil compared to the non-encapsulated fungus. In addition, the preliminary evidence suggests that <em>Tn</em>R-209−SCMs have a basic safety profile for practical applications. Inoculation with <em>Tn</em>R-209−SCMs could effectively promote development and enhance resistance in pigeon pea seedlings by promoting chlorophyll synthesis, improving photosynthesis, and enhancing phenolic compound accumulation. Meanwhile, <em>T. neorugulosus</em> R-209 was found to endogenously colonize root intercellular spaces. Moreover, co-inoculation of <em>Tn</em>R-209−SCMs and <em>R. solani</em> AG4 could reduce host defense responses compared to <em>R. solani</em> AG4-infected roots, as reflected by lower levels of phenolic compound accumulation and pathogenesis-/biosynthesis-related gene expression. Overall, <em>Tn</em>R-209−SCMs is a promising biological agent that can promote development and control root rot in plants, which also provides an innovative approach to biomaterial-supported agricultural practices.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154569"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persicaria minor F-box protein, PmFBK2 targeted by miR156a in response to MeJA treatment, potentially affects stress-related proteins","authors":"Nur-Athirah Abd-Hamid ,&nbsp;Muhammad-Izzat Ahmad-Fauzi ,&nbsp;Ismanizan Ismail","doi":"10.1016/j.jplph.2025.154568","DOIUrl":"10.1016/j.jplph.2025.154568","url":null,"abstract":"<div><div>This study investigates the relationship between microRNAs (miRNAs) and F-box proteins (FBPs) in <em>Persicaria minor</em>, under methyl jasmonate (MeJA) treatment. miRNAs regulate gene expression by targeting mRNAs via cleaving or modifying the mRNAs through base pairing, while FBPs, as part of the SCF (Skp1-Cullin-F-box) complex, mediates protein degradation via the ubiquitin-26S proteasome system (UPS). These post-transcriptional and post-translational regulators work together to activate plant responses. Integrated in silico analysis and experimental validation identified five miRNA–<em>FBP</em> pairs: miR156a–<em>PmFBK2</em>, miR396a–<em>PmFBX1</em>, miR156a/c–<em>PmFBX2</em>, miR408–<em>PmFBK3</em>, and miR398–<em>PmFBL1</em>. Among these, miR156a and miR408 showed negative expression correlations with their <em>FBP</em> targets, further confirmed by RLM-RACE cleavage assays, suggesting direct post-transcriptional regulation. Both target genes encode FBP containing kelch repeat (FBK), a subfamily abundant in plants and associated with stress-responsive pathways. Further analysis of the miR156a target through yeast two-hybrid (Y2H) revealed that, PmFBK2 protein targets SAMS2, PAL1 and GID1, proteins involved in metabolic and hormonal regulation linked to stress responses These findings suggest that miRNA-mediated regulation of <em>FBP</em> may influence protein interaction networks relevant to stress adaptation. This study presents foundational evidence for the involvement of specific miRNA-<em>FBP</em> interactions in plant stress responses, laying the groundwork for future functional validation and crop improvement.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154568"},"PeriodicalIF":4.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}