Journal of plant physiology最新文献

{"title":"Pollen tube-expressed RUPO forms a complex with OsMTD2 and OsRALF17 and OsRALF19 peptides in rice (Oryza sativa)","authors":"Ji-Hyun Kim ,&nbsp;Ye-Jin Son ,&nbsp;Eui-Jung Kim ,&nbsp;Ki-Hong Jung ,&nbsp;Yu-Jin Kim","doi":"10.1016/j.jplph.2025.154421","DOIUrl":"10.1016/j.jplph.2025.154421","url":null,"abstract":"<div><div>Pollen tubes are crucial for angiosperm plants, as they deliver sperm gametes for the essential process of double fertilization. Understanding the molecular mechanisms behind pollen tube germination and growth is critical; however, these processes remain partially elucidated in monocot cereal crops. Rapid Alkalinization Factor (RALF), a small peptide of about 5 kDa, binds to the CrRLK1L receptor and plays a role in various plant physiological processes, including reproduction and tip growth. Recently, we reported that OsRALF17 and OsRALF19 binds to the OsMTD2, pollen specific CrRLK1L member, and regulates pollen tube growth. In this study, we demonstrate that <em>Ruptured Pollen tube (RUPO</em>), another CrRLK1L member, is also a putative receptor for OsRALF17 and OsRALF19, and propose the formation of a receptor complex with OsMTD2. In tobacco epidermal cells, OsMTD2 and RUPO were co-localized at both the plasma membrane (PM) and the nuclear membrane. Additionally, we generated a RUPO-tagged line driven by its native promoter to visualize subcellular localization during pollen tube growth. RUPO localizes a tip-enriched distribution, with intense fluorescence at the tip's PM and cytoplasm in pollen tube. Upon treatment with synthetic OsRALF17M and OsRALF19M peptides, a reduction in the signal near the PM was observed, suggesting a potential response to these peptides. Our data support the role of RUPO as a candidate receptor for OsRALF17 and OsRALF19 in rice pollen tubes, thereby suggesting a novel mechanism for these RALFs in regulating pollen tube function. Additionally, we observed a significant delay in pollen tube burst time upon treatment with synthetic OsRALF17M and synthetic OsRALF19M. We propose that investigating this phenomenon may provide further insights into the specific signaling pathways mediated by these RALFs.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154421"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decrypting proteomics, transcriptomics, genomics, and integrated omics for augmenting the abiotic, biotic, and climate change stress resilience in plants","authors":"Rashmi Choudhary ,&nbsp;Faheem Ahmad ,&nbsp;Cengiz Kaya ,&nbsp;Sudhir Kumar Upadhyay ,&nbsp;Sowbiya Muneer ,&nbsp;Vinod Kumar ,&nbsp;Mukesh Meena ,&nbsp;Haitao Liu ,&nbsp;Hrishikesh Upadhyaya ,&nbsp;Chandra Shekhar Seth","doi":"10.1016/j.jplph.2025.154430","DOIUrl":"10.1016/j.jplph.2025.154430","url":null,"abstract":"<div><div>As our planet faces increasing environmental challenges, such as biotic pressures, abiotic stressors, and climate change, it is crucial to understand the complex mechanisms that underlie stress responses in crop plants. Over past few years, the integration of techniques of proteomics, transcriptomics, and genomics like LC-MS, IT-MS, MALDI-MS, DIGE, ESTs, SAGE, WGS, GWAS, GBS, 2D-PAGE, CRISPR-Cas, cDNA-AFLP, HLS, HRPF, MPSS, CAGE, MAS, IEF, MudPIT, SRM/MRM, SWATH-MS, ESI have significantly enhanced our ability to comprehend the molecular pathways and regulatory networks, involved in balancing the ecosystem/ecology stress adaptation. This review offers thorough synopsis of the current research on utilizing these multi-omics methods (including metabolomics, ionomics) for battling abiotic (salinity, temperature (chilling/freezing/cold/heat), flood (hypoxia), drought, heavy metals/loids), biotic (pathogens like fungi, bacteria, virus, pests, and insects (aphids, caterpillars, moths, mites, nematodes) and climate change stress (ozone, ultraviolet radiation, green house gases, carbon dioxide). These strategies can expedite crop improvement, and act as powerful tools with high throughput and instant database generation rates. They also provide a platform for interpreting intricate, systematic signalling pathways and knowing how different environmental stimuli cause phenotypic responses at cellular and molecular level by changing the expression of stress-responsive genes like <em>RAB18, KIN1, RD29B, OsCIPK03, OsSTL</em>, <em>SIAGL, bZIP, SnRK, ABF</em>. This review discusses various case studies that exemplify the successful implementation of these omics tools to enhance stress tolerance in plants. Finally, it highlights challenges and future prospects of utilizing these approaches in combating stress, emphasizing the need for interdisciplinary collaborations and bio-technological advancements for sustainable agriculture and food security.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154430"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different Agrobacterium rhizogenes strains on hairy root induction and analysis of metabolites in Physalis peruviana L.","authors":"Yijia Zhong ,&nbsp;Zhie Zhou ,&nbsp;Zhongping Yin ,&nbsp;Lu Zhang ,&nbsp;Qingfeng Zhang ,&nbsp;Yihai Xie ,&nbsp;Jiguang Chen","doi":"10.1016/j.jplph.2025.154431","DOIUrl":"10.1016/j.jplph.2025.154431","url":null,"abstract":"<div><div><em>Physalis peruviana</em> L. (<em>P. peruviana</em>) is an edible medicinal plant rich in bioactive phenolics. This study aimed to establish a hairy root (HR) culture of <em>P. peruviana</em> as a potential source for the synthesis of natural compounds. HRs were induced in <em>P. peruviana</em> using different <em>Agrobacterium rhizogenes</em> strains (R1601, C58C1, A4, and K599). Notably, K599 did not induce HR formation, whereas R1601, C58C1, and A4 yielded transformation frequencies of 57.78, 65.14, and 72.31%, respectively. Secondary metabolite production and antioxidant capacity were further examined in HRs induced using C58C1, R1601, and A4. It was found that <em>A. rhizogenes</em> R1601 induced the greatest increase (44% compared to that observed in the non-transformed culture). The methanolic extract of HRs induced by <em>A. rhizogenes</em> R1601 exhibited strong antioxidant capacity, with IC₅₀ values of 1.41 mg DE/mL and 2.33 mg DE/mL for 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), respectively. The HR culture showed higher production of phenolic compounds and higher antioxidant capacity than the non-transformed cultures. Ultra-performance liquid chromatography time-of-flight tandem mass spectrometry was used to identify eight alkaloids, phenolics, and glycoside compounds. <em>A. rhizogenes</em> R1601 is emerging as a possible strain for the mass production of HR and bioactive phenolic compounds in <em>P. peruviana</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154431"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and validation of reference genes for the normalization of RT-qPCR gene expression data in Rheum tanguticum (Polygonaceae) under various abiotic stresses","authors":"Mingjun Yu ,&nbsp;Jiarui Jin ,&nbsp;Xiaoli Li ,&nbsp;Yuping Liu ,&nbsp;Xu Su ,&nbsp;Marcos A. Caraballo-Ortiz ,&nbsp;Penghui Zhang ,&nbsp;Qian Yang ,&nbsp;Rongju Qu ,&nbsp;Zhaxi Cairang ,&nbsp;Xuanlin Gao ,&nbsp;Yinghui Zheng","doi":"10.1016/j.jplph.2025.154435","DOIUrl":"10.1016/j.jplph.2025.154435","url":null,"abstract":"<div><div><em>Rheum tanguticum</em>, an endemic species from the Qinghai-Xizang Plateau, is a significant perennial and medicinal plant recognized for its robust resistance to abiotic stresses, including drought, cold, and salinity. To advance the understanding of stress-response mechanisms in <em>R. tanguticum</em>, this study aimed to establish a reliable set of housekeeping genes as references for normalizing RT-qPCR gene expression analyses. Ten candidate genes were selected based on prior studies of related species and transcriptomic data for <em>R. tanguticum</em>. Their expression stability was evaluated across three tissue types (leaf, stem, and root) under four abiotic stress treatments using a comprehensive suite of bioinformatics tools. Our findings revealed that <em>RtEF1α</em> was the most stable reference gene in salt- and drought-stressed roots and drought-stressed stems, while <em>RtTUB</em> was most stable in salt-stressed stems. <em>RtSAND</em> was identified as the most stable reference gene in salt-, drought-, heavy metal-, and hormone-stressed leaves. For heavy metal- and hormone-stressed roots and heavy metal-stressed stems, <em>RtUBC</em> demonstrated the highest stability, whereas <em>RteIF6A</em> was most stable in hormone-stressed stems. Furthermore, the utility of these reference genes was validated by assessing the expression pattern of the drought-related gene <em>RtNAC</em>. This study is the first report on selecting and validating reference genes of <em>R. tanguticum</em> under various stress conditions, which will benefit future investigations of the genomic mechanisms involved on stress resistance in this species.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154435"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Gln alteration influences leaf morphogenesis by mediating gibberellin levels in tobacco","authors":"Qi-Li Mi ,&nbsp;Hong-Tao Lv ,&nbsp;Fei-Fei Huang ,&nbsp;Li Xu ,&nbsp;Zi-Ying Huang ,&nbsp;Wen-Wu Yang ,&nbsp;Mei-Yun Zou ,&nbsp;Hai-Tao Huang ,&nbsp;Wan-Li Zeng ,&nbsp;Jia-Yin Zhao ,&nbsp;Qing-Xian Chen ,&nbsp;Yong-En Si-Tu ,&nbsp;Hai-Ying Xiang ,&nbsp;Yuan-Qi Jiang ,&nbsp;Zhi-Tong Mai ,&nbsp;Shu-Yuan Ding ,&nbsp;Zhan-Yu Liu ,&nbsp;Bo-Keng Hu ,&nbsp;Liu-Hong Li ,&nbsp;Xue-Mei Li ,&nbsp;Qian Gao","doi":"10.1016/j.jplph.2025.154437","DOIUrl":"10.1016/j.jplph.2025.154437","url":null,"abstract":"<div><div>Nitrogen is a critical nutrient for plant growth and development. While numerous studies have investigated the mechanisms by which nitrate and/or ammonium regulate plant growth, little is known about whether and how amino acids regulate plant leaf development. This study demonstrates that tobacco plants with altered expression levels of an amino acid transporter (LYSINE HISTIDINE TRANSPORTER1, <em>NtLHT1</em>, Ntab0818090) exhibit significant differences in leaf morphology. Knock-out mutants exhibit elongated and narrower leaves compared to wild-type plants, whereas overexpression (OE) lines display orbicular leaves. Additionally, mutant plants exhibit decreased nitrogen use efficiency (NUE) under half MS medium and delayed development under nitrogen-depleted conditions. Moreover, overexpression lines demonstrate better performance. Although the mutant does not show significant lower level of nitrate or total amino acid content in the developing leaves, its amino acid profile, particularly glutamine (Gln), is significantly altered. Supplementation with Gln in the growth medium, rather than glutamate, can restore the morphological differences observed in mutant leaves, suggesting a pivotal role of Gln in regulating leaf shape. To further elucidate the mechanisms underlying leaf shape regulation, we analyzed endogenous hormone levels and applied exogenous hormones to these lines. Our findings suggest that amino acids transported from source organs, particularly glutamine (Gln), play a key role in controlling leaf development and morphology through the modulation of multiple phytohormones, such as gibberellic acid (GA).</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154437"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effects of warming on carbon budget, photosynthetic yield and biochemical composition of cold-temperate and Arctic isolates of Laminaria digitata (Phaeophyceae)","authors":"Angelika Graiff ,&nbsp;Kiara Franke ,&nbsp;Ulf Karsten ,&nbsp;Daniel Liesner ,&nbsp;Francisco J.L. Gordillo ,&nbsp;Concepción Iñiguez","doi":"10.1016/j.jplph.2025.154436","DOIUrl":"10.1016/j.jplph.2025.154436","url":null,"abstract":"<div><div>Cold-temperate and Arctic hard bottom coastal ecosystems are dominated by kelp forests, which have a high biomass production and provide important ecosystem services, but are subject to change due to ocean warming. However, the photophysiological response to increasing temperature of ecologically relevant species, such as <em>Laminaria digitata</em>, might depend on the local thermal environment where the population has developed. Therefore, the effects of temperature on growth rate, biochemical composition, maximum quantum yield, photosynthetic quotient and carbon budget of young cultured sporophytes of <em>Laminaria digitata</em> from the Arctic at Spitsbergen (SPT; cultured at 4, 10 and 16 °C) and from the cold-temperate North Sea island of Helgoland (HLG; cultured at 10, 16 and 22 °C) were comparatively analyzed. Temperature significantly affected growth rates of <em>L. digitata</em> from SPT and HLG, with the highest rates occurring at 10 °C, but growth did not differ between both isolates neither at 10 °C nor at 16 °C. Nevertheless, maximum quantum yield and carbon fixation rate were highest at 4 °C for the Arctic and at 16 °C for the cold-temperate <em>L. digitata</em>. Significantly higher rates of oxygen production and carbon fixation were observed in the cold-temperate relative to the Artic <em>L</em>. <em>digitata</em> at 10 and 16 °C, respectively. Neither temperature nor biogeographic region of origin affected the photosynthetic quotient, and release rates of dissolved or particulate organic carbon. Total carbon and mannitol content were significantly higher in the Arctic compared to the cold-temperate <em>L. digitata</em> at 10 °C, revealing an increased accumulation of storage compounds in the high latitude <em>L. digitata</em>. We conclude that <em>L. digitata</em> from SPT and HLG differ in their sensitivity to increasing temperatures and that the Arctic population from Spitsbergen is likely to benefit from ocean warming, while the temperate population from Helgoland will be negatively affected by further increases in ambient temperature.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154436"},"PeriodicalIF":4.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative study of subcellular distribution, chemical forms, and physiological responses for understanding cadmium tolerance in two garden shrubs","authors":"Shiyin Yu,&nbsp;Shan Wang,&nbsp;Min Tang,&nbsp;Shuzhen Pan,&nbsp;Meixian Wang","doi":"10.1016/j.jplph.2025.154419","DOIUrl":"10.1016/j.jplph.2025.154419","url":null,"abstract":"<div><div>Urban ornamental shrubs have significant potential for restoring cadmium (Cd)-contaminated soil. The Cd enrichment characteristics and tolerance mechanisms of <em>Buxus sinica</em> and <em>Ligustrum</em> × <em>vicaryi</em> were investigated through a simulated pot pollution experiment. Specifically, the Cd content and accumulation in different plant tissues, the subcellular distribution and chemical forms of Cd in the roots, and the effects of Cd on the ultrastructure of root cells under various Cd concentrations (0, 25, 50, 100, and 200 mg kg⁻¹) were analyzed. The results showed that: (1) As the Cd treatment levels increased, the total biomass of <em>B. sinica</em> gradually decreased, while <em>L.</em> × <em>vicaryi</em> exhibited a stimulation effect at low Cd concentrations but inhibition at high Cd concentrations. (2) The Cd content in different tissues of both shrubs increased with rising Cd levels. The bioconcentration factor (BCF) and translocation factor (TF) indicated that <em>L.</em> × <em>vicaryi</em> has the potential for Cd phytostabilization. (3) Cd in the roots of both shrubs was primarily present in NaCl-extractable form, and was mostly bound to the cell wall. (4) Excessive Cd caused damage to the cellular structure of <em>B. sinica</em>, while the cells of <em>L.</em> × <em>vicaryi</em> maintained normal morphology. (5) In both shrubs, Cd primarily bound to the cell wall through hydroxyl and amino functional groups, as well as soluble sugars. In summary, converting Cd to less active forms, immobilizing Cd in the cell wall, and providing binding sites through functional groups may be crucial resistance mechanisms for both shrubs in response to Cd stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154419"},"PeriodicalIF":4.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastid- and photoreceptor-dependent signaling is required for the response to photoperiod stress","authors":"I. Bajaj Hengge,&nbsp;Anne Cortleven,&nbsp;Thomas Schmülling","doi":"10.1016/j.jplph.2025.154429","DOIUrl":"10.1016/j.jplph.2025.154429","url":null,"abstract":"<div><div>Prolongation of the light period causes photoperiod stress in plants. The response to photoperiod stress includes the induction of a distinct set of stress marker genes, of reactive oxygen species (ROS), and of stress hormones. In this study, the impact of light intensity and light quality on the photoperiod stress response was investigated. A threshold light intensity of circa 50 μmol m⁻² s⁻¹ is necessary for inducing photoperiod stress, indicating the involvement of chloroplasts. Lower photoperiod stress symptoms in retrograde signaling mutants (<em>gun4</em>, <em>gun5</em>) and mutants with constrained plastid function (<em>glk1 glk2</em>) corroborated the role of chloroplasts. Genetic analysis revealed that the photoreceptors phyB and particularly CRY2 are important to perceive photoperiod stress. Overall, these results showed that both plastid-dependent and photoreceptor-dependent signaling pathways are involved in sensing the light conditions causing photoperiod stress and governing the response to it.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154429"},"PeriodicalIF":4.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning the promoter of the sucrose transporter gene PsSUT2 and screening its upstream transcription factors in tree peony","authors":"Mengjuan Chai,&nbsp;Jingjing Han,&nbsp;Qianru Yan,&nbsp;Renxuan Xue,&nbsp;Jiuxing Lu,&nbsp;Yonghua Li,&nbsp;Yan Li","doi":"10.1016/j.jplph.2024.154410","DOIUrl":"10.1016/j.jplph.2024.154410","url":null,"abstract":"<div><div>Sucrose is an essential energy substance for tree peony (Paeonia Suffruticosa) floral organ development. However, little is known about the sucrose regulatory network in tree peony. In this study, the promoter sequence of the tree peony sucrose transporter gene <em>PsSUT2</em> was cloned. Through cis-acting elements analysis and weighted gene co-expression network analysis (WGCNA), 6 transcription factors potentially regulating <em>PsSUT2</em> were screened. Expression analysis revealed that the 6 transcription factors had similar expression trends with the <em>PsSUT2</em> in all parts of peony at the full bloom stage. Furthermore, a yeast one-hybrid assay revealed that PsMYB20 and PsMADS9 bind to the <em>PsSUT2</em> promoter. Dual-luciferase reporter assay demonstrated that PsMYB20 and PsMADS9 could activate <em>PsSUT2</em> expression. Taken together, our findings suggest that PsMYB20 and PsMADS9 positively regulate <em>PsSUT2</em>, laying the foundation for the construction of a gene network for sucrose regulation in tree peony.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154410"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of energy level on reactive oxygen species metabolism during shade-drying of Thompson seedless grapes and its relation to browning","authors":"Yujing Wang ,&nbsp;Yuchen Zhang ,&nbsp;Wei Huang ,&nbsp;Zhen zhen Xu ,&nbsp;Miaomiao Zhang ,&nbsp;Xuetong Zhang ,&nbsp;Cheng Wang ,&nbsp;Fengjuan Liu","doi":"10.1016/j.jplph.2024.154403","DOIUrl":"10.1016/j.jplph.2024.154403","url":null,"abstract":"<div><div>The browning of Thompson seedless grapes during shade-drying significantly hampers the sustainable and healthy development of the industry. This study investigates the browning phenomenon and reactive oxygen species (ROS) dynamics when Thompson seedless grapes, treated with adenosine triphosphate (ATP), 2,4-dinitrophenol (DNP), and water (QS), are dried in the shade. The effects of these treatments on ROS metabolism were analyzed through physiological, biochemical, and proteomic analyses. The findings showed that ATP treatment markedly delayed the increase in browning and reactive oxygen content, maintained high activity levels of ROS scavenging enzymes (superoxide dismutase and peroxidase), reduced malondialdehyde production—a membrane lipid peroxidation product—and preserved cell membrane integrity compared to QS and DNP treatments. Proteomic analysis identified three biological pathways involved in ROS metabolism in Thompson seedless grapes: glutathione metabolism, ascorbic acid, and glyoxalate metabolism, and peroxisomal pathways. Exogenous ATP treatment upregulated the expression of 17 proteins (SOD, APX, GPX, GST, GR), with significant increases in GST2 (D7SKQ2), POD1 (F6H095), SOD3 (D7TI74), and SOD4 (F6HTX9) by 1.707, 1.589, 1.644, and 2.213-fold, respectively. Therefore, ATP treatment maintains ROS scavenging proteins’ expression, reduces the accumulation of ROS, maintains a balance in ROS metabolism, maintains the cell membrane stability and suppresses the oxidation of lipids, thus delaying the browning of Thompson seedless grapes. These findings are significant for regulating browning in the shade-drying process of Thompson seedless grapes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154403"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}