Journal of plant physiology最新文献

{"title":"Sucrose synthase: An enzyme with multiple roles in plant physiology","authors":"Jinling Li,&nbsp;Yingying Hu,&nbsp;Jiajia Hu,&nbsp;Qingmin Xie,&nbsp;Xuehao Chen,&nbsp;Xiaohua Qi","doi":"10.1016/j.jplph.2024.154352","DOIUrl":"10.1016/j.jplph.2024.154352","url":null,"abstract":"<div><div>Sucrose synthase (SuS) is a key enzyme in the regulation of sucrose metabolism in plants and participates in the reversible reaction of sucrose conversion to uridine diphosphate-glucose and fructose. It plays an important role in promoting taproot development, starch synthesis, cellulose synthesis, improving plant nitrogen fixation capacity, sugar metabolism, and fruit and seed development. Recent studies have shown that SuS responds to abiotic stresses such as drought stress, cold stress and waterlogging stress, especially in waterlogging stress. This paper provides a comprehensive review on the basic properties, physiological functions, and signal transduction pathways of SuS, aiming to establish a theoretical foundation for its further research.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154352"},"PeriodicalIF":4.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of StERECTA enhances drought tolerance in Arabidopsis thaliana","authors":"Xuan Liu ,&nbsp;Wenjing Yang ,&nbsp;Li Zhang ,&nbsp;Fengjie Nie ,&nbsp;Lei Gong ,&nbsp;Hongxia Zhang","doi":"10.1016/j.jplph.2024.154353","DOIUrl":"10.1016/j.jplph.2024.154353","url":null,"abstract":"<div><div>Drought is a major abiotic stresses that severely hinder plant growth and agricultural productivity. The receptor-like kinase gene, <em>ERECTA</em>, has been proved to play important role in promoting the response to abiotic stress in crops. However, the specific molecular mechanisms underlying the drought resistance mediated by <em>ERECTA</em> in potato (<em>Solanum tuberosum</em> L.) are not well understood. In this study, sequence analysis confirmed that the <em>StERECTA</em> gene contains eight leucine-rich repeat (LRR) domains and an S_TKc domain, and these domains were highly conserved in <em>Solanaceae</em> family. Under drought stress, <em>Arabidopsis thaliana</em> strains overexpressing <em>StERECTA</em> showed increased biomass, proline (PRO) content, and antioxidant enzyme activities compared to the wild-type strains while the mutant <em>ERECTA</em> strain (<em>er105</em>) exhibited opposite phenotype. Additionally, <em>StERECTA</em> overexpression upregulated the expression of drought response marker genes (<em>LEA3</em>, <em>DREB2A</em> and <em>P5CS1</em>), improved levels of ABA and auxin, reduced stomatal density and relative expression level of stomatal development related genes <em>(SPCH</em>, <em>FAMA</em> and <em>MUTE</em>). Furthermore, Co-immunoprecipitation (Co-IP) assays demonstrated that <em>StERECTA</em> physically interacted with the YODA protein. In conclusion, our study provides new insights into the role and regulatory mechanism of <em>StERECTA</em> in response to drought stress. These findings may serve as a basis for genetic improvement of potato to enhance their tolerance to abiotic stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154353"},"PeriodicalIF":4.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPOTLIGHT: TaSPL6-D, a transcriptional repressor of TaHKT1;5-D in bread wheat (Triticum aestivum L.) and a novel target for improving salt tolerance in crops","authors":"Devrim Coskun","doi":"10.1016/j.jplph.2024.154351","DOIUrl":"10.1016/j.jplph.2024.154351","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154351"},"PeriodicalIF":4.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724001822/pdfft?md5=3af4ce46e2d715af6c71466a1144658d&pid=1-s2.0-S0176161724001822-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation of tomato xyloglucan transglucosylase/hydrolase5 increases fruit firmness and contributes to prolonged shelf life","authors":"Shuai Yuan ,&nbsp;Xin Gou ,&nbsp;Jing Hu,&nbsp;Chaowen Xiao,&nbsp;Juan Du","doi":"10.1016/j.jplph.2024.154350","DOIUrl":"10.1016/j.jplph.2024.154350","url":null,"abstract":"<div><p>Fruit ripening in tomato is a highly coordinated developmental process accompanied with fruit softening, which is closely associated with cell wall degradation and remodeling. Xyloglucan endotransglucosylase/hydrolases (XTHs) are known to play an essential role in cell wall xyloglucan metabolism. Tomato XTH5 exhibits xyloglucan endotransglucosylase (XET) activity <em>in vitro</em>, but the understanding of its biological role in fruit ripening remains unclear. In this study, we revealed that <em>SlXTH5</em> is highly expressed in mature fruits. Knockout mutant plants of <em>SlXTH5</em> were generated by CRISPR/Cas9 gene editing strategy in tomato cultivar Micro-Tom. The mutant fruits showed accelerated transition from unripe to ripe process and earlier ethylene accumulation compared to wild type fruits. Although the mutation of <em>SlXTH5</em> did not affect the size, weight and number of fruits, it indeed increased fruit firmness and extended shelf life, which is probably attributed to the increased cell layer and cell wall thickness of pericarp tissue. Pathogen infection experiment showed the enhanced resistance of mutant fruits to <em>Botrytis cinerea</em>. These results revealed the role of SlXTH5 in fruit ripening process, and provide new insight into how cell wall metabolism and remodeling regulate fruit softening and shelf life.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154350"},"PeriodicalIF":4.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low nitrogen priming enhances Rubisco activation and allocation of nitrogen to the photosynthetic apparatus as an adaptation to nitrogen-deficit stress in wheat seedling","authors":"Huimin Chai,&nbsp;Lijun Gao,&nbsp;Chengfeng Zhao,&nbsp;Xiaoxue Liu,&nbsp;Dong Jiang,&nbsp;Tingbo Dai,&nbsp;Zhongwei Tian","doi":"10.1016/j.jplph.2024.154337","DOIUrl":"10.1016/j.jplph.2024.154337","url":null,"abstract":"<div><p>Reducing nitrogen (N) application is crucial in addressing the low N utilization efficiency (NUE) and the risks of environmental pollution in wheat production. Improving low N (LN) tolerance in wheat can help balance the conflict between wheat growth and reduced N fertilization. Hydroponic experiments were conducted using Yangmai158 (LN-tolerant) and Zaoyangmai (LN-sensitive) cultivars to study whether LN priming (LNP) in the 3-leaf stage can improve the photosynthetic capacity of wheat seedlings under N-deficit stress at the 5-leaf stage. LNP increased the net photosynthetic rate (<em>P</em>_n), stomatal conductance (<em>G</em>_s), electron transfer rate (ETR), carboxylation efficiency (CE), maximum carboxylation rate (<em>V</em>_cmax), and the content and activity of Rubisco and Rubisco activase (RCA) in both cultivars, with Yangmai158 showing a greater increase than Zaoyangmai. After 14 days of N-deficit stress, the decreases in <em>P</em>_n, <em>G</em>_s, ETR, CE, <em>V</em>_cmax, and the content and activity of Rubisco and RCA of the two cultivars treated with LNP were significantly lower compared with those of the treatments without LNP. LNP improved the allocation proportion of leaf N to photosynthetic machinery, with the greatest increase in the carboxylation machinery. These results indicate that LNP can allocate more N to the photosynthetic apparatus, improving Rubisco content and activity to enhance the photosynthetic capacity and NUE of leaves under N-deficit stress.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154337"},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The crosstalk interaction of ethylene, gibberellins, and arbuscular mycorrhiza improves growth in salinized tomato plants by modulating the hormonal balance","authors":"Mouna Khalloufi ,&nbsp;Cristina Martínez-Andújar ,&nbsp;Najoua Karray-Bouraouib ,&nbsp;Francisco Pérez-Alfocea ,&nbsp;Alfonso Albacete","doi":"10.1016/j.jplph.2024.154336","DOIUrl":"10.1016/j.jplph.2024.154336","url":null,"abstract":"<div><p>Ethylene (ET) and gibberellins (GAs) play key roles in controlling the biotic and abiotic interactions between plants and environment. To gain insights about the role of ET and GAs interactions in the mycorrization and response to salinity of tomato (<em>Solanum lycopersicum</em> L.) plants, the ET-insensitive (<em>Never-ripe</em>, <em>Nr</em>), and the ET-overproducer (<em>Epinastic, Epi</em>) mutants and their wild type cv. Micro-Tom (MT), were inoculated or not with the arbuscular mycorrhizal fungi (AMF) <em>Rhizophagus irregularis</em> and exposed to control (0 mM NaCl) and salinity (100 mM NaCl) conditions, with and without gibberellic acid (10⁻⁶ M GA₃) application during four weeks. Exogenous GA₃ enhanced plant growth irrespective of the genotype, AMF, and salinity conditions, while an additional effect on growth by AMF was only found in the ET-overproducer (<em>Epi</em>) mutant under control and salinity conditions. <em>Epi</em> almost doubled the AMF colonization level under both conditions but was the most affected by salinity and GA₃ application compared to MT and <em>Nr</em>. In contrast, <em>Nr</em> registered the lowest AMF colonization level, but GA₃ produced a positive effect, particularly under salinity, with the highest leaf growth recovery. Foliar GA₃ application increased the endogenous concentration of GA₁, GA₃, and total GAs, more intensively in AMF-<em>Epi</em> plants, where induced levels of the ET-precursor ACC were also found. Interestingly, GA₄ which is associated with AMF colonization, registered the strongest genotype x GA x AMF × salinity interactions. The different growth responses in relation to those interactions are discussed.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154336"},"PeriodicalIF":4.0,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724001676/pdfft?md5=ca61791fb5bd4ceba31e0bcddeabd5b3&pid=1-s2.0-S0176161724001676-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The zinc-finger transcription factor ZFP8 negatively regulates the drought stress response in Arabidopsis thaliana by inhibiting the transcriptional activity of ABF2","authors":"Yu'ang Tian ,&nbsp;Yanling Li ,&nbsp;Kelan Wang,&nbsp;Ran Xia,&nbsp;Yingru Lin,&nbsp;Guohui Pan,&nbsp;Haoyu Shi,&nbsp;Dawei Zhang,&nbsp;Honghui Lin","doi":"10.1016/j.jplph.2024.154338","DOIUrl":"10.1016/j.jplph.2024.154338","url":null,"abstract":"<div><p>Drought stress limits plant growth and development. To cope with drought stress, abscisic acid (ABA) accumulates in plants. Although ABA-dependent drought tolerance pathways have been widely investigated, the feedback mechanisms and the negative regulatory roles within these pathways remain largely unknown. Here we characterize the roles of a C2H2 transcription factor, ZFP8, whose expression is repressed by ABA in the tolerance of drought stress. <em>ZFP8</em>-overexpressing plants were hyposensitive to ABA and exhibited less dehydration tolerance while ABA or drought-induced marker genes were more highly expressed in <em>zfp8</em>, suggesting that ZFP8 functions as a negative regulator in the ABA-mediated drought response. A transcriptome assay showed that ZFP8 positively regulates gene expression for cellular function and negatively regulates hormone and stress response gene expression. Moreover, we found that ZFP8 can interact with ABF2, one of the basic leucine zipper (bZIP) family transcription factor members, to inhibit its transcription activity. In conclusion, our results demonstrate a novel negative regulation pathway of ZFP8, which contributes to plants’ ability to fine-tune their drought responses.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154338"},"PeriodicalIF":4.0,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus deficiency promotes root:shoot ratio and carbon accumulation via modulating sucrose utilization in maize","authors":"Zu-Dong Xiao ,&nbsp;Zhen-Yuan Chen ,&nbsp;Yi-Hsuan Lin ,&nbsp;Xiao-Gui Liang ,&nbsp;Xin Wang ,&nbsp;Shou-Bing Huang ,&nbsp;Sebastian Munz ,&nbsp;Simone Graeff-Hönninger ,&nbsp;Si Shen ,&nbsp;Shun-Li Zhou","doi":"10.1016/j.jplph.2024.154349","DOIUrl":"10.1016/j.jplph.2024.154349","url":null,"abstract":"<div><p>Phosphorus deficiency usually promotes root:shoot ratio and sugar accumulation. However, how the allocation and utilization of carbon assimilates are regulated by phosphorus deficiency remains unclear. To understand how phosphorus deficiency affects the allocation and utilization of carbon assimilates, we systematically investigated the fixation and utilization of carbon, along with its diurnal and spatial patterns, in hydroponically grown maize seedlings under low phosphorus treatment. Under low phosphorus, sucrolytic activity was slightly inhibited by 12.0% in the root but dramatically inhibited by 38.8% in the shoot, corresponding to the promoted hexose/sucrose ratio and biomass in the root. Results point to a stable utilization of sucrose in the root facilitating competition for more assimilates, while increasing root:shoot ratio. Moreover, starch and sucrose accumulated in the leaves under low phosphorus. Spatially, starch and sucrose were oppositely distributed, starch mainly in the leaf tip, and sucrose mainly in the leaf base and sheath. Evidence of sucrose getting stuck in leaf base and sheath suggests that carbon accumulation is not attributed to carbon assimilation or export disturbance, but may be due to poor carbon utilization in the sinks. These findings improve the understanding of how low phosphorus regulates carbon allocation between shoot and root for acclimation to stress, and highlight the importance of improving carbon utilization in sinks to deal with phosphorus deficiency.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154349"},"PeriodicalIF":4.0,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The germin-like protein OsGLP8-7 is involved in lignin synthesis for acclimation to copper toxicity in rice","authors":"Tengwei Xiao ,&nbsp;Xiangchao ShangGuan ,&nbsp;Yu Wang ,&nbsp;Zhonghe Tian ,&nbsp;Kejian Peng ,&nbsp;Zhenguo Shen ,&nbsp;Zhubing Hu ,&nbsp;Yan Xia","doi":"10.1016/j.jplph.2024.154335","DOIUrl":"10.1016/j.jplph.2024.154335","url":null,"abstract":"<div><p>Although copper (Cu) is an essential microelement for plant growth and development, excess Cu results in a dramatic reduction in crop yield and quality. In the present study, we report that rice germin-like protein 8-7 (OsGLP8-7) plays a crucial role against Cu toxicity. The results showed that the transcriptional expression of the <em>OsGLP8-7</em> gene was remarkably upregulated in the root and leaf by Cu treatment. The depletion of <em>OsGLP8-7</em> significantly decreased the elongation of the primary root and plant height of rice under excess Cu. This hypersensitivity of <em>osglp8-7</em> mutants towards excess Cu may be attributed to the weaker Cu retention in the cell wall compared with wild-type rice (Dongjin, DJ). Consistently, Cu-induced phenylpropanoid biosynthesis was compromised in <em>osglp8-7</em> mutants based on RNA-Seq and qRT-PCR analysis. Furthermore, <em>osglp8-7</em> mutants displayed a reduction of lignin deposition in the cell wall, and subsequently altered cell morphology. <em>Osglp8-7</em> mutant lines also had higher Cu-induced O₂^•⁻ and H₂O₂ levels than those of DJ under Cu stress. The results suggest that OsGLP8-7 participates in lignin synthesis for the acclimation to excess Cu. These findings provide a better understanding of a novel mechanism of germin-like proteins in the alleviation of heavy metal toxicity in rice.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154335"},"PeriodicalIF":4.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small molecule inhibitors of human LRRK2 enhance in vitro embryogenesis and microcallus formation for plant regeneration of crop and model species","authors":"Elena Carneros ,&nbsp;Eduardo Berenguer ,&nbsp;Yolanda Pérez-Pérez ,&nbsp;Saurabh Pandey ,&nbsp;Ralf Welsch ,&nbsp;Klaus Palme ,&nbsp;Carmen Gil ,&nbsp;Ana Martínez ,&nbsp;Pilar S. Testillano","doi":"10.1016/j.jplph.2024.154334","DOIUrl":"10.1016/j.jplph.2024.154334","url":null,"abstract":"<div><p><em>In vitro</em> plant embryogenesis and microcallus formation are systems which are required for plant regeneration, a process during which cell reprogramming and proliferation are critical. These systems offer many advantages in breeding programmes, such as doubled-haploid production, clonal propagation of selected genotypes, and recovery of successfully gene-edited or transformed plants. However, the low proportion of reprogrammed cells in many plant species makes these processes highly inefficient. Here we report a new strategy to improve <em>in vitro</em> plant cell reprogramming using small molecule inhibitors of mammalian leucine rich repeat kinase 2 (LRRK2), which are used in pharmaceutical applications for cell reprogramming, but never used in plants before. LRRK2 inhibitors increased <em>in vitro</em> embryo production in three different systems and species, microspore embryogenesis of oilseed rape and barley, and somatic embryogenesis in cork oak. These inhibitors also promoted plant cell reprogramming and proliferation in Arabidopsis protoplast cultures. The benzothiazole derivative JZ1.24, a representative compound of the tested molecules, modified the expression of the brassinosteroid (BR)-related genes <em>BIN2, CPD</em>, and <em>BAS1</em>, correlating with an activation of BR signaling. Additionally, the LRRK2 inhibitor JZ1.24 induced the expression of the embryogenesis marker gene <em>SERK1-like</em>. The results suggest that the use of small molecules from the pharmaceutical field could be extended to promote <em>in vitro</em> reprogramming of plant cells towards embryogenesis or microcallus formation in a wider range of plant species and <em>in vitro</em> systems. This technological innovation would help to develop new strategies to improve the efficiency of <em>in vitro</em> plant regeneration, a major bottleneck in plant breeding.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154334"},"PeriodicalIF":4.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724001652/pdfft?md5=3b8407529c011b378462bb491483053f&pid=1-s2.0-S0176161724001652-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}