Journal of plant physiology最新文献

{"title":"Laser microdissection and fluorescence in situ hybridization reveal the tissue-specific gene expression in the ovules of P. tabulaeformis Carr","authors":"Xinyu Zhang ,&nbsp;Binli Chen ,&nbsp;Xiaoxin Song ,&nbsp;Yingqi Wang ,&nbsp;Caixia Zheng ,&nbsp;Zaixin Gong","doi":"10.1016/j.jplph.2025.154500","DOIUrl":"10.1016/j.jplph.2025.154500","url":null,"abstract":"<div><div>Ovules are important carriers for seed plant reproduction, and ovules of gymnosperms are composed mainly of female gametophyte (FG) and adjacent diploid tissue (ADT). To investigate tissue-specific genes in the ovules of <em>Pinus tabulaeformis</em> Carr., we used laser microdissection (LMD) to separate FGs and ADTs, and performed linear amplification to construct cDNA libraries, obtaining a total of 156 expressed sequence tags (EST). Furthermore, some differentially expressed genes between FG and ADT of <em>P. tabulaeformis</em> ovule were screened by the analysis of EST. In addition, the expression levels of key genes in fertile line (FL) and sterile line (SL) ovules during development were verified by RT-qPCR, and we found that both <em>PtRPL7a</em> and <em>PtDHN4</em> were more highly expressed in FL in each period (at least 1.7 times that of SL). Finally, fluorescence in situ hybridization (FISH) was used to reveal the temporal and spatial expression patterns of <em>PtRPL7a</em> and <em>PtDHN4</em> in the ovules of <em>P. tabuliformis</em> during ovule development between FL and SL. Our results indicate that the expression levels and the locations of <em>PtRPL7a</em> and <em>PtDHN4</em> show significant differences in different tissues during ovule development between FL and SL. This study further elucidates the molecular mechanism of the ovule abortion of <em>P. tabulaeformis</em> and provides a theoretical basis for the germplasm optimization of gymnosperms.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"309 ","pages":"Article 154500"},"PeriodicalIF":4.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AmCERK1 and AmLYK3 interaction mediates CIP-induced defense responses in A. macrocephala","authors":"Lei Chen ,&nbsp;Xuyan Fu ,&nbsp;Kun Wu ,&nbsp;Xiangbing Chang ,&nbsp;Wei Tian","doi":"10.1016/j.jplph.2025.154497","DOIUrl":"10.1016/j.jplph.2025.154497","url":null,"abstract":"<div><div>Southern blight caused by <em>Sclerotium rolfsii</em> (<em>S. rolfsii</em>) represents a significant threat to the medicinal plant <em>Atractylodes macrocephala</em> Koidz. (<em>A. macrocephala</em>), with effective control measures remaining limited. <em>Chrysanthemum indicum</em> polysaccharides (CIP) have been identified as an elicitor capable of inducing defense responses in <em>A. macrocephala</em> against <em>S. rolfsii</em> infection. However, the molecular mechanisms underlying CIP recognition remain poorly understood. In this study, comparative transcriptome analysis revealed two potential <em>LysM</em>-receptor kinases, Am<em>CERK1</em> and Am<em>LYK3</em>, as candidate receptors for CIP recognition. These genes, which are orthologous to <em>Arabidopsis CERK1</em> and <em>Medicago truncatula LYK3</em>, exhibited significant up-regulation upon CIP treatment. Bimolecular fluorescence complementation (BiFC) assays demonstrated that Am<em>CERK1</em> and Am<em>LYK3</em> interact in a CIP-dependent manner. Transient overexpression experiments further confirmed that CIP treatment markedly enhanced the expression of these receptor genes. Virus-induced gene silencing (VIGS) assays indicated that CIP treatment could partially compensate for the suppression of Am<em>CERK1</em> and Am<em>LYK3</em>, highlighting their critical role in CIP-induced defense responses. Collectively, these findings suggest that Am<em>CERK1</em> and Am<em>LYK3</em> form a pattern recognition receptor (PRR) complex essential for CIP perception, potentially facilitating pattern-triggered immunity (PTI) in <em>A. macrocephala</em>. These findings reveal a novel receptor recognition complex comprising Am<em>CERK1</em> and Am<em>LYK3</em>, offering crucial insights into the mechanisms of innate immune recognition in plants.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154497"},"PeriodicalIF":4.0,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthophyll cycle and photosynthetic electron transport enhanced by galactolipid modification alleviate drought-induced leaf senescence","authors":"Jili Xu ,&nbsp;Guanqiang Zuo ,&nbsp;Shuaikang Liu ,&nbsp;Hao Shi ,&nbsp;Lina Yin ,&nbsp;Shiwen Wang ,&nbsp;Xiping Deng","doi":"10.1016/j.jplph.2025.154499","DOIUrl":"10.1016/j.jplph.2025.154499","url":null,"abstract":"<div><div>Previous studies have demonstrated galactolipid modification was involved in drought-induced leaf senescence. Under drought stress, overactivation of the photosynthetic electron transfer chain leads to excessive light energy absorption, resulting in photooxidative damage to crops. The xanthophyll cycle, a key photoprotective mechanism, mitigates light-induced damage by dissipating excess energy as heat. However, the role of the xanthophyll cycle pigments and photosynthetic electron transport in the process of galactolipid modification alleviates drought-induced leaf senescence has not yet been clarified clearly. In this study, a comparative experiment was conducted to investigate changes in the xanthophyll cycle and photosynthetic electron transport during drought and re-watering in two maize varieties: a drought-tolerant variety (Liangyu66) and a senescent variety (Liangyu99). Drought stress induced more severely wilted and leaf senescence in Liangyu99, with lower shoot biomass, photosynthetic rate, chlorophyll <em>a</em>/b, monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) content, corresponding gene expression level and DGDG/MGDG ratio compared to Liangyu66. Furthermore, PSII electron transport rate (ETRⅡ), the PSI electron transport rate (ETRⅠ), and cyclic electron flow (CEF) in Liangyu66 were 14 %, 47 %, and 83 % higher, respectively, than in Liangyu99 under drought stress. Notably, the de-epoxidation state of the xanthophyll cycle (A + Z)/(A + Z + V) was significantly higher in Liangyu66 than in Liangyu99. Non-photochemical quenching (NPQ) increased in both varieties under drought stress, Liangyu66 displayed a higher NPQ than Liangyu99. These findings suggest that galactolipid modification alleviates drought-induced leaf senescence by enhancing the xanthophyll cycle and optimizing photosynthetic electron transport.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154499"},"PeriodicalIF":4.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illuminating plant metabolism with genetically encoded biosensors","authors":"Stephan Wagner,&nbsp;Andreas J. Meyer","doi":"10.1016/j.jplph.2025.154498","DOIUrl":"10.1016/j.jplph.2025.154498","url":null,"abstract":"<div><div>The metabolic flexibility of plants enables them to cope particularly well with changing environmental conditions. This flexibility is achieved by cellular processes that require tight coordination in space and time and constant balancing to maximise plant fitness. If we want to identify crops with higher yields and improved resistance to abiotic and biotic stresses, then we need to unravel these metabolic processes experimentally, and genetically encoded biosensors (GEBs) seem ideal for this. They allow non-invasive monitoring of metabolic processes in living cells over time and with high spatial and temporal resolution. The list of sensors and sensor variants that have been developed or established in plants continues to grow, providing insights into more and more parameters of plant metabolism. This, together with technological advances, also facilitates paraplexing and multiplexing experiments, where several processes are monitored simultaneously by GEBs. Despite these advantages, GEBs need to be used carefully and users must fully understand their characteristics in the chosen experimental plant system in order to draw meaningful conclusions from the spectroscopic changes of a sensor. Here, we aim to provide a list of fluorescent GEBs that can be selected for <em>in planta</em> use and highlight recent biological insights gained from them, focusing on advances where multiple GEBs have been used. We also discuss criteria for selecting an appropriate sensor and aspects of the field that remain challenging, in the hope of helping plant scientists to generate and interpret plant metabolism data using GEBs in a meaningful way.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154498"},"PeriodicalIF":4.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome guided treasure hunt - learning from metabolic diversity","authors":"Esra Karakas,&nbsp;Mustafa Bulut,&nbsp;Alisdair Fernie","doi":"10.1016/j.jplph.2025.154494","DOIUrl":"10.1016/j.jplph.2025.154494","url":null,"abstract":"<div><div>Metabolomics is a rapidly evolving field focused on the comprehensive identification and quantification of small molecules in biological systems. As the final layer of the biological hierarchy following of the genome, transcriptome and proteome, it presents a dynamic snapshot of phenotype, influenced by genetic, environmental and physiological factors. Whilst the metabolome sits downstream of genes and proteins, there are multiple higher levels—tissues, organs, the entire organism, and interactions with other organisms, which need to be considered in order to fully comprehend organismal biology. Advances in metabolomics continue to expand its applications in plant biology, biotechnology, and natural product discovery unlocking many of nature's most beneficial colors, tastes, nutrients and medicines. Flavonoids and other specialized metabolites are essential for plant defense against oxidative stress and function as key phytonutrients for human health. Recent advancements in gene-editing and metabolic engineering have significantly improved the nutritional value and flavor of crop plants. Here we highlight how advanced metabolic analysis is driving improvements in crops uncovering genes that influence nutrient and flavor profile and plant derived compounds with medicinal potential.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"309 ","pages":"Article 154494"},"PeriodicalIF":4.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of FvGCN5 enhances the resistance of woodland strawberry against Botrytis cinerea","authors":"Miao Yu,&nbsp;Jiaqi Zhang,&nbsp;Feifei Bai,&nbsp;Yihan Gao,&nbsp;Shan Jiang,&nbsp;Hui Liu,&nbsp;Aisheng Xiong,&nbsp;Zongming Cheng,&nbsp;Jinsong Xiong","doi":"10.1016/j.jplph.2025.154496","DOIUrl":"10.1016/j.jplph.2025.154496","url":null,"abstract":"<div><div>Epigenetic modifications mediated by histone acetylation play essential roles in plant development and stress response. However, the mechanism of regulating biotic stress through histone acetyltransferase GCN5 in strawberry is still unclear. In this study, we isolated <em>FvGCN5</em> from woodland strawberry and found that FvGCN5 may form the conserved SAGA (Spt-Ada-Gcn5 acetyltransferase) complex through interaction with FvADA2a and FvADA2b. In addition, we found that <em>FvGCN5</em> could be significantly induced by the infection of fungal pathogen <em>Botrytis cinerea</em>, and that the transgenic strawberry plants overexpressing <em>FvGCN5</em> exhibited enhanced resistance against <em>B. cinerea</em>. Further RNA-seq-based transcriptome and quantitative PCR analysis indicated that several disease-resistant genes such as <em>FvMYC2</em> and, <em>FvPR1</em> were significantly upregulated in <em>FvGCN5</em> overexpression lines. Taken together, our study indicates that <em>FvGCN5</em> plays important roles in the resistance against <em>B. cinerea</em> in woodland strawberry through activating disease-resistant genes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154496"},"PeriodicalIF":4.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptome and metabolome analyses reveal the molecular mechanism of re-flowering induction in Hydrangea macrophylla","authors":"Haixia Chen ,&nbsp;Huijun Zhang ,&nbsp;Denghui Wang ,&nbsp;Yajing Wang,&nbsp;Hui Jiang,&nbsp;Jiren Chen","doi":"10.1016/j.jplph.2025.154492","DOIUrl":"10.1016/j.jplph.2025.154492","url":null,"abstract":"<div><div>Flowering duration is pivotal for ornamental appeal, with re-flowering being essential for prolonging the decorative period and enhancing the aesthetics of flowers. We conducted transcriptome and metabolome sequencing analyses on the primary and secondary flower buds of <em>H. macrophylla</em> cv 'White Angel', aiming to reveal the molecular regulatory mechanism of secondary flowering. Results showed that the key MADS-box transcription factor family genes closely related to flowering regulation such as <em>AGL42</em>, <em>AGL24</em>, and <em>SVP</em> demonstrated a substantial increase in expression levels within the secondary flower buds. The up-regulation of these genes may promote the transition from vegetative growth to reproductive growth by regulating the expression of downstream target genes, thus triggering secondary flowering. In addition, genes related to starch and sucrose metabolism (such as TPS and TPP) were significantly overexpressed in secondary flower buds, promoting the accumulation of energy metabolites such as Trehalose-6p, Trehalose and D-Glucose, which may create conditions for secondary flowering by providing necessary energy support. At the same time, terpenoid biosynthesis-related genes (such as <em>KO</em>, <em>KAO</em>, <em>GA2ox</em> and <em>GA3ox)</em> were highly expressed in secondary flower buds, significantly increasing the contents of GA4 and GA7, while decreasing the level of GA3. These dynamic changes of gibberellins (GAs) may regulate the expression of flowering related genes. Further promote the occurrence of secondary flowering. In summary, this study revealed the synergistic effect of genes and metabolites in the regulation of secondary flowering of <em>Hydrangea macrophylla</em> 'White Angel', and the MADS-box transcription factor directly promoted the transformation of reproductive growth through up-regulated expression. The accumulation of starch, sucrose and its derivatives and gibberellin metabolites may trigger the secondary flowering process of plants through energy supply and hormone signal regulation. These findings provide a new perspective for in-depth analysis of the flowering regulation mechanism of <em>Hydrangea macrophylla</em>, and lay a theoretical foundation for further cultivation of horticultural varieties with excellent ornamental characteristics.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154492"},"PeriodicalIF":4.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halophilic Bacillus improve barley growth on calcareous soil via enhanced photosynthetic performance and metabolomic re-programing","authors":"Tarek Slatni ,&nbsp;Walid Zorrig ,&nbsp;Amal Razzegui ,&nbsp;José Antonio Hernández ,&nbsp;Gregorio Barba-Espín ,&nbsp;Karim Ben Hamed ,&nbsp;Pedro Díaz-Vivancos","doi":"10.1016/j.jplph.2025.154495","DOIUrl":"10.1016/j.jplph.2025.154495","url":null,"abstract":"<div><div>Plant Growth Promoting Rhizobacteria are a sustainable biological alternative to agrochemicals to improve plant growth and stress tolerance. In this work we used two <em>Bacillus</em> strains native to the saline rhizosphere of halophytic plants in order to improve the growth of barley on a calcareous soil (CS). This soil negatively affected plant development; however, the inoculation of barley with the halophytic <em>Bacillus</em> strains enhanced barley growth and photosynthesis performance. In fact, a significant increase of the maximum photochemical yield of PSII and PSI was observed following inoculation, leading to improved protection of these photosystems against photoinhibition. Moreover, a pairwise metabolomic pathway analysis in barley leaves and roots was performed. Compared to barley grown on non-calcareous soil (NCS), CS led to a downregulation of sugar-related metabolic pathways, which can be correlated with lower photosynthesis performance. Furthermore, the abundance of metabolites related to amino acids in leaves and phenylpropanoids and lipids in roots was also reduced by CS. This negative effect was reverted by the inoculation of the bacteria strains. In conclusion, halophilic <em>Bacillus</em> native to the saline rhizosphere of halophyte plants induced metabolic changes leading to an enhanced photosynthesis activity, and hence, alleviating the deleterious effect of CS on barley development.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"309 ","pages":"Article 154495"},"PeriodicalIF":4.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory network of lncRNAs and mRNAs explains why salinity promotes photosynthesis and plant growth in the halophyte Suaeda salsa","authors":"Chenyang Li ,&nbsp;Runtai Zhao ,&nbsp;Bing Cui ,&nbsp;Ranran Liu ,&nbsp;Chaoran Shi ,&nbsp;Jie Song","doi":"10.1016/j.jplph.2025.154493","DOIUrl":"10.1016/j.jplph.2025.154493","url":null,"abstract":"<div><div><em>Suaeda salsa</em> L. exhibits strong salt tolerance, with 200 mM NaCl being the optimum salt concentration for its growth. However, the specific molecular regulatory network remains unclear. This study used high-throughput sequencing technology to identify the expression abundance of lncRNAs after 24 h of 200 mM NaCl treatment (S24). A total of 16533 novel_lncRNAs were obtained, mainly divided into 10764 lincRNAs (65.11 %), 4936 antisense (29.85 %), and 833 sense overlap (5.04 %). Comparing lncRNAs at S24 and CK revealed 231 up-regulated and 257 down-regulated lncRNAs detected. The differential target genes corresponding to lncRNAs were mainly enriched in carbon metabolism, glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms and glyoxylate and dicarboxylate metabolism. Comparing the mRNAs at S24 and CK, the up-and down-regulated genes were 998 and 776, respectively, which corresponded to those for lncRNAs. Further investigation revealed that a particular lncRNA, TCONS_00024624 (lnc24), interacts with three genes that collectively regulate ribulose bisphosphate carboxylase (Rubisco). The expression of target genes of lncRNAs and activity of Rubisco and GAPDH in the leaves of <em>S. salsa</em> were upregulated and increased at S24 compared with CK. In conclusion, the results suggest that lncRNAs play important roles in enhancing the photosynthetic capacity of <em>S. salsa</em> and promoting its growth at 200 mM NaCl. This provides new references for studying salt tolerance mechanisms in <em>S. salsa</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154493"},"PeriodicalIF":4.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is the redox state of the PQ pool involved in regulating the ET biosynthesis pathway of CAM facultative semi-halophytes?","authors":"Miron Gieniec ,&nbsp;Zbigniew Miszalski ,&nbsp;Piotr Rozpądek ,&nbsp;Roman J. Jędrzejczyk ,&nbsp;Isabel Nogues ,&nbsp;Emanuele Pallozzi ,&nbsp;Walter Stefanoni ,&nbsp;Michał Nosek","doi":"10.1016/j.jplph.2025.154491","DOIUrl":"10.1016/j.jplph.2025.154491","url":null,"abstract":"<div><div>The mechanisms and factors regulating ethylene (ET) biosynthesis and its action remain largely unexplored, particularly in CAM-facultative and (semi)halophytic plants. The use of <em>Mesembryanthemum crystallinum</em> L. (ice plant) provides a unique opportunity to examine plastoquinone (PQ) - ET interactions in semi-halophytes and CAM-facultative plants simultaneously. Here, we present the results of an analysis of the common ice plant's response to prolonged (14-day) salinity stress and DCMU, which maintains the PQ pool in a more oxidised state, thereby mimicking darkness conditions. Differentially expressed gene (DEG) analysis showed that the expression of genes involved in ET regulation was not significantly altered after DCMU application. However, in C₃ plants not affected by salinity stress, the expression of genes related to both photosystems, photosynthesis, and the photosynthetic electron transport chain was significantly affected by DCMU. We propose that sustained salinity stress and the occurrence of CAM photosynthesis render physiological processes insensitive to disruptions caused by a modified PQ pool redox state. The UPLC-MS analysis of the ET biosynthesis pathway central intermediate – 1-aminocyclopropane-1-carboxylic acid (ACC) – confirmed the molecular analysis results, as ACC content was similarly affected in salinity untreated and treated plants. Moreover, the analysis of key antioxidative system components, namely catalase and superoxide dismutases, suggests that PQ pool redox state does not directly regulate them. Instead, an alternative regulation mechanism involving reactive oxygen species (ROS) accumulation and a ROS-induced signalling cascade has been proposed.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154491"},"PeriodicalIF":4.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}