Journal of plant physiology最新文献_第5页

Towards genetic architecture and genomic prediction of crop traits from time-series data: Challenges and breakthroughs 基于时间序列数据的作物性状遗传结构与基因组预测：挑战与突破

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-11 DOI: 10.1016/j.jplph.2025.154566

David Hobby , Alain J. Mbebi , Zoran Nikoloski

{"title":"Towards genetic architecture and genomic prediction of crop traits from time-series data: Challenges and breakthroughs","authors":"David Hobby , Alain J. Mbebi , Zoran Nikoloski","doi":"10.1016/j.jplph.2025.154566","DOIUrl":"10.1016/j.jplph.2025.154566","url":null,"abstract":"<div><div>Advances in remote and proximal sensing have facilitated temporal high-throughput phenotyping of crop populations grown in field conditions. The resulting time-series phenotypic data capture single or multiple growth- and yield-related traits at different temporal resolution. Whilst classical quantitative genetics approaches can readily be used with these temporal data by considering the measurement of a character at a given time point as a separate trait, this strategy fully neglects inter-trait integration over time. Here, we provide a classification of computational approaches that can be used to effectively analyze temporal phenotyping data from crop populations, focusing on genomic prediction, identification of quantitative trait loci, and genome-wide association studies. We point out the existing challenges due to the consideration of time-resolved data, and stress the extent to which these challenges are addressed by the available computational solutions. Finally, we highlight recent breakthroughs that make use of time-resolved data for multiple traits and are poised to revolutionize breeding efforts of climate-resilient crops.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154566"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revising the role of ABA as regulator of flowering and seed development 修正ABA在开花和种子发育调控中的作用

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-10 DOI: 10.1016/j.jplph.2025.154565

Anna Collin, Agata Daszkowska-Golec

引用次数: 0

Salinity-induced changes in the PSII/LHCII phosphorylation and organization of the photosynthetic protein complexes in the halophyte Mesembryanthemum crystallinum L 盐生植物中PSII/LHCII磷酸化和光合蛋白复合物组织的变化

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-10 DOI: 10.1016/j.jplph.2025.154567

Maria Pilarska , Wioleta Wasilewska-Dębowska , Ewa Niewiadomska

{"title":"Salinity-induced changes in the PSII/LHCII phosphorylation and organization of the photosynthetic protein complexes in the halophyte Mesembryanthemum crystallinum L","authors":"Maria Pilarska , Wioleta Wasilewska-Dębowska , Ewa Niewiadomska","doi":"10.1016/j.jplph.2025.154567","DOIUrl":"10.1016/j.jplph.2025.154567","url":null,"abstract":"<div><div>Halophytes have been widely used to investigate plant salt tolerance, but the mechanisms regulating photosynthesis under salinity are still poorly understood. Here, the effect of 10-day NaCl irrigation on the phosphorylation status of photosystem II (PSII), a light-harvesting complex of PSII (LHCII) and the organization of protein complexes in thylakoids of the halophyte <em>Mesembryanthemum crystallinum</em> L. (common ice plant) was investigated. In salt-acclimated plants, increased phosphorylation of LHCB1, LHCB2 and D1 proteins was observed under dark and light conditions. This was accompanied by reduced capability to perform state transitions in response to different light quality, as inferred from changes in the steady-state chlorophyll <em>a</em> fluorescence. Low-temperature chlorophyll fluorescence emission spectra revealed decreased PSII fluorescence in the dark and light in salt-acclimated plants, with a significantly smaller decrease of PSI fluorescence in the dark than in well-watered controls. These data indicate, that salinity reduces nocturnal LHCII disconnection from PSI, resulting in a permanent State II. The decrease in the functional PSII antenna in salt-acclimated plants was confirmed by the parameters of the fast kinetics of chlorophyll <em>a</em> fluorescence. Furthermore, blue native polyacrylamide gel electrophoresis revealed salinity-induced partial disassembly of PSII supercomplexes. Our results show that changes in the PSII/LHCII phosphorylation levels and the organization of thylakoid protein complexes play a role in acclimation to salinity in this halophytic species.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154567"},"PeriodicalIF":4.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylobacterium oryzae as a growth biostimulant of Arabidopsis thaliana and Solanum lycopersicum 拟南芥和番茄的生长促生长剂米化甲基杆菌

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-10 DOI: 10.1016/j.jplph.2025.154564

Zamłyńska Katarzyna , Suśniak Katarzyna , Samborska Dorota , Komaniecka Iwona , Łabuć Emilia , Choma Adam

{"title":"Methylobacterium oryzae as a growth biostimulant of Arabidopsis thaliana and Solanum lycopersicum","authors":"Zamłyńska Katarzyna , Suśniak Katarzyna , Samborska Dorota , Komaniecka Iwona , Łabuć Emilia , Choma Adam","doi":"10.1016/j.jplph.2025.154564","DOIUrl":"10.1016/j.jplph.2025.154564","url":null,"abstract":"<div><div><em>Methylobacterium</em> spp. bacteria occur commonly in the environment. The presence of some methylobacteria in the soil/plant have positive effect to the plants growth and can reduce or prevent the consequence of phytopathogens. We determined the effect of <em>M. oryzae</em> CBMB20 (rice endosymbiont) on different stages of <em>Arabidopsis thaliana</em> and <em>Solanum lycopersicum</em> development. Protective properties against phytopathogenic bacteria of <em>M. oryzae</em> CBMB20 lipopolysaccharide were also determined. High resolution mass spectrometry was used to confirm presence of IAA in tomato extracts. Based on the obtained results we concluded that, <em>M. oryzae</em> CBMB20 had no significant effect on the germination percentage of both plants but increased the number of root hairs in <em>A. thaliana</em> and the length of <em>S. lycopersicum</em> sprouts and led to an increase in the fresh weight of the plants. LPS CBMB20 was able to strengthen a defence reaction in response to the presence of the phytopathogen. <em>S. lycopersicum,</em> treated with CBMB20, produced more IAA than plants that were not treated with the methylobacteria, which translates into an increase in fresh mass. These findings suggest that <em>M. oryzae</em> CBMB20 has potential as a component of biopreparations.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154564"},"PeriodicalIF":4.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of AtBES1D in tomato enhances BR response and accelerates fruit ripening AtBES1D在番茄中过表达可增强BR反应，加速果实成熟

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-09 DOI: 10.1016/j.jplph.2025.154563

Sen He , Xiubo Xia , Junhui Yang , Jinyang Xin , Siqi Chen , Chengguo Jia

{"title":"Overexpression of AtBES1D in tomato enhances BR response and accelerates fruit ripening","authors":"Sen He , Xiubo Xia , Junhui Yang , Jinyang Xin , Siqi Chen , Chengguo Jia","doi":"10.1016/j.jplph.2025.154563","DOIUrl":"10.1016/j.jplph.2025.154563","url":null,"abstract":"<div><div>Brassinosteroids (BRs) are essential plant hormones that regulate growth and development, with BRI1-EMS SUPPRESSOR 1 (BES1) and BRASSINAZOLE-RESISTANT 1 (BZR1) serving as central transcription factors in BR signaling. However, the role of BES1 in regulating tomato fruit ripening remains poorly understood. Here, we generated three independent transgenic tomato lines overexpressing <em>Arabidopsis thaliana BES1D</em> (<em>AtBES1D</em>). Overexpression of <em>AtBES1D</em> enhanced BR responses, as demonstrated by enhanced responsiveness to BRs and reduced sensitivity to the BR biosynthesis inhibitor brassinazole (BRZ). <em>AtBES1D-</em>transgenic tomato plants exhibited pleiotropic phenotypic alterations, including stunted growth, curled leaves, suppressed root elongation, delayed flowering, accelerated fruit ripening, and diminished fruit size, weight, and seed number. In addition, <em>AtBES1D</em> transgenic fruits exhibited upregulated expression of ethylene-related genes (<em>ACS4</em>, <em>NR</em>, <em>ERF1</em>, <em>ERF4</em>, <em>E4</em>, and <em>E8</em>) and ripening regulators (<em>RIN</em>, <em>TAGL1</em>, <em>FUL1</em>, <em>FUL2</em>, and <em>PG</em>). Chromatin immunoprecipitation sequencing (ChIP-seq) identified 1757 AtBES1D target genes, predominantly enriched in plant hormone signaling, transcriptional regulation, and metabolic pathways. Collectively, these findings establish AtBES1D as a multifunctional regulator modulating vegetative development, reproductive transition, and fruit ripening in tomato. AtBES1D likely promotes fruit ripening and improves fruit quality by modulating BR signaling, ethylene pathways, transcription factors, and metabolic processes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154563"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and fine mapping of qTGW11, a QTL conferring high nitrogen use efficiency in Dongxiang wild rice (Oryza rufipogon Griff.) 东乡野生稻氮素高效QTL qTGW11的鉴定与精细定位

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-05 DOI: 10.1016/j.jplph.2025.154562

Yumin Shen , Wentao Xiong , Aiping Shu , Lanxiang Hu , Shiyou Luo , Jintao Huang , Huanjin Xiong , Xiaoyan Wu , Yeqing Xiao , Mingliang Chen

{"title":"Identification and fine mapping of qTGW11, a QTL conferring high nitrogen use efficiency in Dongxiang wild rice (Oryza rufipogon Griff.)","authors":"Yumin Shen , Wentao Xiong , Aiping Shu , Lanxiang Hu , Shiyou Luo , Jintao Huang , Huanjin Xiong , Xiaoyan Wu , Yeqing Xiao , Mingliang Chen","doi":"10.1016/j.jplph.2025.154562","DOIUrl":"10.1016/j.jplph.2025.154562","url":null,"abstract":"<div><div>The extensive utilization of synthetic nitrogen fertilizers has substantially increased crop yields while severely disrupting the ecological balance. Consequently, enhancing nitrogen use efficiency in crops has become imperative for sustainable agricultural development. Dongxiang wild rice (DXWR), demonstrating remarkable tolerance to low-nitrogen stress, represents a precious germplasm resource for breeding nitrogen-efficient rice cultivars. In this study, we conducted quantitative trait loci (QTL) mapping for plant height, effective panicle number, grain number per panicle, grain yield per plant, and thousand-grain weight under low-nitrogen and normal-nitrogen conditions using 150 backcross recombinant inbred lines (BILs) derived from a cross between the <em>indica</em> maintainer line GanxiangB and DXWR, with <strong>a genetic linkage map comprising 153 SSR markers</strong>. Of 23 QTLs identified across 11 chromosomes, 9 were consistently detected under both nitrogen conditions. A stable QTL <em>qTGW11</em> was identified under both nitrogen conditions; explaining 8.37–9.57 % of the phenotypic variation. Through map-based cloning, <em>qTGW11</em> was precisely localized to a 117-kb genomic region harboring 16 candidate genes, among which <em>LOC_Os11g40100</em> was identified as the most likely causal gene through quantitative reverse transcription PCR (qRT-PCR) validation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154562"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting biochemical compositions and microbial interactions of English oak and black poplar root mucilage 英国橡树和黑杨根粘液的生化成分和微生物相互作用对比

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-07-01 DOI: 10.1016/j.jplph.2025.154561

Meisam Nazari , Mathilde Knott , Yakov Kuzyakov , Marta Sena-Velez , Sylvain Bourgerie , Sabine Carpin , Frédéric Lamblin

{"title":"Contrasting biochemical compositions and microbial interactions of English oak and black poplar root mucilage","authors":"Meisam Nazari , Mathilde Knott , Yakov Kuzyakov , Marta Sena-Velez , Sylvain Bourgerie , Sabine Carpin , Frédéric Lamblin","doi":"10.1016/j.jplph.2025.154561","DOIUrl":"10.1016/j.jplph.2025.154561","url":null,"abstract":"<div><div>Root mucilage plays a crucial role in plant-soil interactions, yet its composition and functions for trees remain largely unexplored. We investigated the root mucilage of two tree species with contrasting growth strategies: the slow-growing English oak (<em>Quercus robur</em> L.) and the fast-growing black poplar (<em>Populus nigra</em> L.). Our analyses focused on the polysaccharide composition of mucilage and its microbial interactions. English oak mucilage polysaccharides consisted of 54% galactose, 16% mannose, 11% arabinose, 7% xylose, and 12% glucuronic acid, with no detectable glucose or galacturonic acid. In contrast, black poplar mucilage polysaccharides contained 26% galactose, 14% mannose, 14% glucose, 22% arabinose, 7% xylose, 14% glucuronic acid, and 3% galacturonic acid. Both mucilage types were hexose-rich, resembling the hexose-to-pentose ratio common in microbial sources in soil. Black poplar mucilage had a higher uronic acid-to-neutral monosaccharide ratio and greater K<sup>+</sup> and Na<sup>+</sup> concentrations than English oak mucilage. Functionally, black poplar mucilage increased the growth of <em>Pseudomonas fluorescens</em> SBW25, suggesting the provision of readily available carbon sources. Conversely, English oak mucilage suppressed bacterial growth, plausibly due to antimicrobial compounds that may slow microbial decomposition and promote carbon sequestration.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154561"},"PeriodicalIF":4.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive genetic dissection of yield-related traits utilizing quantitative trait loci sequencing approach in mungbean 利用数量性状位点测序方法对绿豆产量相关性状进行综合遗传剖析

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-27 DOI: 10.1016/j.jplph.2025.154552

Fan Yang , Jiaqi Qiao , Bin Li , Xiao Zhang , Dongye Liu , Bojing Wang , Aili Wei , Dongao Huo

{"title":"Comprehensive genetic dissection of yield-related traits utilizing quantitative trait loci sequencing approach in mungbean","authors":"Fan Yang , Jiaqi Qiao , Bin Li , Xiao Zhang , Dongye Liu , Bojing Wang , Aili Wei , Dongao Huo","doi":"10.1016/j.jplph.2025.154552","DOIUrl":"10.1016/j.jplph.2025.154552","url":null,"abstract":"<div><div>Mungbean [<em>Vigna radiata</em> (L.) R. Wilczek] has gained significant popularity in the food industry, due to its distinctive functional properties and exceptional nutritional value. Increasing yield is a central objective in mungbean breeding programs; however, systematic studies identifying quantitative trait loci (QTLs) associated with key yield-related traits remain limited. In this study, the recombinant inbred line (RIL) population (AH20 × SX36) was generated, and phenotypic assessments were conducted in three distinct environments. Three methods genome-wide composite interval mapping (GCIM), multiple QTL mapping (MQM) and inclusive composite interval mapping (ICIM) were employed to detect QTLs linked to HSW (hundred-seed weight), SPP (number of seeds per pod), PL (pod length), PW (pod width), and YP (yield per plant). Consequently, 33, 19, 26, 22, and 20 QTLs were identified for HSW, SPP, PL, PW, and YP, respectively. Notably, 10 QTLs were consistently detected across all environments and by all three mapping methods, indicating their robustness and potential for breeding applications. Candidate genes associated with these stable QTLs were also predicted, offering insights into the genetic regulation of yield traits. These findings provide a valuable genetic framework for functional validation and the cultivation of high-yielding mungbean germplasm.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154552"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MeCNL3-MeARF6 module plays an important role in cassava bacterial blight resistance MeCNL3-MeARF6模块在木薯抗白叶枯病中起重要作用

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-19 DOI: 10.1016/j.jplph.2025.154551

Yingying Li , Rongjiao Zheng , Mengwei Liang , Huiping Zhao , Debing Liu , Guoyin Liu

{"title":"The MeCNL3-MeARF6 module plays an important role in cassava bacterial blight resistance","authors":"Yingying Li , Rongjiao Zheng , Mengwei Liang , Huiping Zhao , Debing Liu , Guoyin Liu","doi":"10.1016/j.jplph.2025.154551","DOIUrl":"10.1016/j.jplph.2025.154551","url":null,"abstract":"<div><div>Cassava bacterial blight (CBB) poses a substantial threat to the progression and sustainability of the cassava industry. While the NLR gene family is known to play a crucial role in plant disease resistance by encoding intracellular immune receptors, the specific molecular mechanisms underlying NLR-mediated resistance in cassava remain poorly understood and require comprehensive characterization. Our research identified MeCNL3, a CC-NBS-LRR resistance gene, demonstrating significant upregulation in response to <em>Xanthomonas axonopodis</em> pv. <em>manihotis</em> (<em>Xam</em>) infection. Functional characterization revealed that <em>MeCNL3</em> overexpression confers enhanced resistance to <em>Xam</em> in cassava. Meanwhile, we demonstrated that MeCNL3 physically interacts with the transcription factor MeARF6, forming a regulatory module that controls <em>Xam</em> resistance. Notably, experimental evidence confirms that MeARF6 regulates <em>MeRbohH</em> transcription, orchestrating reactive oxygen species (ROS)-mediated defense responses against CBB. Furthermore, the type III effector protein XopR hijacks MeCNL3 to suppress the MeCNL3-MeARF6 signaling module, thereby weakening CBB resistance. Taken together, this work delineates the molecular mechanism of MeCNL3-driven CBB immunity and advances the understanding of NLR regulatory networks in cassava defense responses.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154551"},"PeriodicalIF":4.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WEE1 homolog positively regulates salt stress tolerance in chrysanthemum WEE1同源物正调控菊花耐盐性

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-17 DOI: 10.1016/j.jplph.2025.154550

Yahui Wang , Fengze Sun , Zhilong Bao, Fangfang Ma

{"title":"WEE1 homolog positively regulates salt stress tolerance in chrysanthemum","authors":"Yahui Wang , Fengze Sun , Zhilong Bao, Fangfang Ma","doi":"10.1016/j.jplph.2025.154550","DOIUrl":"10.1016/j.jplph.2025.154550","url":null,"abstract":"<div><div>Salinity is a major abiotic stress that limits chrysanthemum yields worldwide. Salinity represses <em>Chrysanthemum lavandulifolium</em> plant growth and consequently reduces chrysanthemum commercial production. Salinity triggers DNA damage in root cells, leading to cell death and subsequent growth repression. <em>WEE1</em> plays an important role in regulating DNA repair, although its function in salt tolerance has not been studied in <em>C. lavandulifolium</em>. In this study, we identify <em>WEE1</em> homologous genes in <em>Chrysanthemum</em> species, and their expressions are induced in roots after salt stress treatment. We further investigate the function of <em>C. lavandulifolium</em> homolog <em>ClWEE1</em> in salt stress responses and find that <em>ClWEE1</em> plays a crucial role in cell cycle regulation and DNA damage repair under salt stress. Overexpressing <em>ClWEE1</em> in <em>C. lavandulifolium</em> or <em>Arabidopsis</em> significantly enhances their salt stress tolerance. Both flow cytometric analysis and comet assay reveal less DNA damage in <em>ClWEE1</em>-overexpression plants than in wild type. RT-qPCR analysis indicates that the stress-responsive genes <em>ClNHX</em>, <em>ClHKT</em>, <em>ClCBL</em>, and <em>ClDREB2A</em> may have higher expression in <em>ClWEE1</em>-overexpression plants than in wild type. Taken together, our study illustrates the positive role of <em>Chrysanthemum WEE1</em> in enhancing salt tolerance, providing insights for breeding salt-tolerant chrysanthemum varieties.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154550"},"PeriodicalIF":4.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0