农杆菌介导的羊草遗传转化及CRISPR/ cas9基因编辑的建立

IF 4 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2025-05-05 DOI:10.1016/j.jplph.2025.154513

Cheng Li , Xiaomei Peng , Zhengshe Zhang , Yaling Liu , García-Caparrós Pedro , Chunxiang Fu , Yongping Yang , Quanmin Dong , Yuanwen Duan , Xudong Sun

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引用次数: 0

摘要

羊草（Elymus nutans）是一种具有StStHHYY基因组的异源六倍体（2n = 6x = 42）物种，是青藏高原高寒草原的原生多年生牧草，是高原上产量最高的牧草，被广泛用于人工草地和生态恢复。然而，由于缺乏稳定的转化体系，制约了花生性状改良的进一步努力。在本研究中，我们建立了可靠的农杆菌介导的花生遗传转化系统，并利用CRISPR/Cas9系统成功生成了entcp4编辑的植株。在E. nutans中，编辑效率达到19.23%。敲除EnTCP4显著延迟开花时间，增强抗亏水胁迫能力。本研究在花生的遗传转化和基因编辑方面取得了重大突破，为深入了解花生的基因功能和分子育种奠定了技术基础。

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Establishment of Agrobacterium-mediated genetic transformation and CRISPR/Cas9-guided gene editing in Elymus nutans

Elymus nutans, an allohexaploid (2n = 6x = 42) species with a StStHHYY genome, is a native perennial in the alpine grasslands of the Qinghai-Xizang Plateau, and has been widely used for artificial pasture and ecological restoration as a forage grass with highest yield on the plateau. Nevertheless, the lack of a stable transformation system has impeded further efforts to trait improvement of E. nutans. In the present study, we established a reliable Agrobacterium-mediated genetic transformation system for E. nutans, and successfully generated EnTCP4-edited plants using the CRISPR/Cas9 system. The editing efficiency achieved 19.23 % in E. nutans. Knocking out EnTCP4 significantly delayed flowering and enhanced water-deficit stress resistance. This research represents a significant breakthrough in the genetic transformation and gene editing of E. nutans, laying a technological foundation to gain insight into gene functions and molecular breeding in E. nutans.

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.