Journal of Photochemistry and Photobiology A-chemistry最新文献

{"title":"Highly selective and sensitive N-amidothiourea-based fluorescence chemosensor for detecting Zn2+ ions and cell Imaging: Potential applications for plasma membrane detection","authors":"Crystal Liu ,&nbsp;Katlin Ricks ,&nbsp;Muneeb Akhtar ,&nbsp;Sergio Mendez ,&nbsp;Ian Lian ,&nbsp;Zhi-Fo Guo","doi":"10.1016/j.jphotochem.2024.116148","DOIUrl":"10.1016/j.jphotochem.2024.116148","url":null,"abstract":"<div><div>A “turn-on” fluorescent sensor <strong>L</strong> was developed for the selective detection of Zn²⁺ ions in biological settings. This sensor exhibited a specific response to Zn²⁺ ions, with a detection limit of 39 nM. Analyses through titration and Job’s plot confirmed that the formed complex has a 2:1 stoichiometry (<strong>L</strong>: Zn²⁺). The sensor displayed strong selectivity for Zn²⁺ over other metal ions, demonstrating a robust binding affinity while effectively reducing interference from heavy metals. The identification process was validated by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (¹H NMR) titrations, mass spectrometry (MS), and Density Functional Theory (DFT) calculations. Maximum fluorescence intensity was achieved upon interaction with Zn²⁺ ions within a physiological pH range of 7.0–7.5. Moreover, successful imaging experiments in HEK293 cells highlighted the sensor’s potential as a powerful tool for monitoring interactions at the plasma membrane.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116148"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solar ultraviolet radiation exposure of trail runners in an ultraendurance competition at high altitude","authors":"J.V. Gutiérrez-Manzanedo ,&nbsp;C. Vaz-Pardal ,&nbsp;A. Rodríguez-Martínez ,&nbsp;J. Aguilera ,&nbsp;P. Gutiérrez-Mulas ,&nbsp;J.L. González-Montesinos ,&nbsp;A. Subert ,&nbsp;F. Rivas-Ruiz ,&nbsp;M. de Troya-Martín","doi":"10.1016/j.jphotochem.2024.116139","DOIUrl":"10.1016/j.jphotochem.2024.116139","url":null,"abstract":"<div><h3>Background</h3><div>Outdoor sports are associated with overexposure to solar ultraviolet radiation and sunburn.</div></div><div><h3>Aims</h3><div>To quantify solar ultraviolet radiation (UVR) exposure received by trail runners during an ultraendurance competition at high altitude and to assess their sun exposure habits, sun protection behaviors, and attitudes and knowledge regarding skin cancer.</div></div><div><h3>Methods</h3><div>Trail runners taking part in the 2023 Ultra Sierra Nevada race (Spain, April 14–16, 2023) completed an online validated questionnaire on sun-related habits, behaviors, attitudes, and knowledge. Environmental conditions such as temperature, relative humidity, and UVR as well as participants’ personal erythemal dose were measured during the competition.</div></div><div><h3>Results</h3><div>The average ambient cumulative erythemal dose ranged from 59.9 to 19.3 standard erythemal doses (SED) and the mean effective radiation received by the athletes studied (n = 17) ranged from 24.2 to 7.6 SED in the Extreme (154 km) to Half-Marathon (25.1 km) races. The questionnaire was completed by 194 athletes (mean age 41.3 ± 8.9 years; 76.8 % men). A total of 22.4 % of athletes surveyed reported skin phototype I-II. Two-thirds (66.5 %) had sunburn after doing outdoor sports in the previous year. In regard to photoprotection practices while exercising outdoors, 62.6 % reported wearing a hat/cap, 59.2 % avoided the midday sun, 38.7 % used sunscreen with sun protection factor ≥ 15, and 49.7 % stated they did not reapply sunscreen.</div></div><div><h3>Conclusions</h3><div>The trail runners studied received a high solar exposure dose during the competition. Future interventions are needed to improve individual and organizational photoprotection measures in trail-running events to reduce the risk of skin cancer.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116139"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR-induced antimicrobial efficacy of TPA-BOIMPY conjugate through photothermal and photodynamic synergy","authors":"Worakrit Saiyasombat ,&nbsp;Sineenat Sripattanakul ,&nbsp;Sastiya Kampaengsri ,&nbsp;Kantapat Chansaenpak ,&nbsp;Rung-Yi Lai ,&nbsp;Anyanee Kamkaew","doi":"10.1016/j.jphotochem.2024.116136","DOIUrl":"10.1016/j.jphotochem.2024.116136","url":null,"abstract":"<div><div>Microbial infections, particularly those produced by multidrug-resistant bacteria, are a major risk to global wellness. In place of conventional antibiotics, photothermal therapy (PTT) and photodynamic therapy (PDT) use light-activated antimicrobial agents to transform near-infrared (NIR) light into heat and reactive oxygen species (ROS), respectively, which effectively eradicate pathogens. This study explored the potential of a new organic dye, bis-(borondifluoride)-8-imidazodipyrromethene (BOIMPY), as a NIR PTT/PDT agent. To increase its phototherapy characteristics, triphenylamines (TPA) were conjugated to BOIMPY to yield <strong>TPA-BOIMPY</strong>, and Pluronic F127 was utilized to improve the hydrophilicity of <strong>TPA-BOIMPY</strong> by forming <strong>TPA-BOIMPY@F127</strong> nanoparticles with an average particle size of 79 nm. These nanoparticles exhibited a maximum absorption peak at 757 nm, a photothermal conversion efficiency of 34 %, a singlet oxygen quantum yield of 0.02, and excellent photostability. Under 808 nm NIR irradiation, <strong>TPA-BOIMPY@F127</strong> remarkably reduced the viability of both <em>E. coli</em> and <em>S. aureus</em> to 0.4% and 7.3%, respectively. The exceptional photostability and promising PTT/PDT capabilities of <strong>TPA-BOIMPY@F127</strong> highlight its potential as a new class of NIR PTT/PDT agents for combating bacterial infections, contributing to the ongoing development of innovative therapeutic strategies.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116136"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive investigation of donor-dcceptor anthraquinone derivatives as versatile and efficient photosensitisers for dye-sensitised solar cells","authors":"Ricardo C. Pereira,&nbsp;Marta Pineiro,&nbsp;J. Sérgio Seixas de Melo","doi":"10.1016/j.jphotochem.2024.116150","DOIUrl":"10.1016/j.jphotochem.2024.116150","url":null,"abstract":"<div><div>A new metal-free anthraquinone donor–acceptor-π-anchor (D-A-π-A) design was developed using unsymmetrically substituted anthraquinone (ATQ) derivatives functionalized with bromine (Br), diphenylamine (DPA), indoline (Ind), <strong>BrATQBzOH</strong>, <strong>DPAATQBzOH</strong>, and <strong>IndATQBzOH</strong>. The synthesised compounds, functionalized with an anchor group (ethynylbenzoic acid), have been evaluated in practical dye-sensitised solar cell (DSSC) devices to improve electron injection efficiency. Their properties and applicability were analysed and rationalised based on their energy levels and parameters derived from the current–voltage (I-V) curve analysis in real devices. Femtosecond transient absorption measurements of the sensitiser <strong>IndATQBzOH</strong> with TiO₂ revealed distinct excited state dynamics and charge transfer properties, highlighting the influence of the semiconductor interface. In addition, Density Functional Theory (DFT) and Time-Dependent DFT (TDDFT) electronic quantum calculations were performed, which revealed that the new anthraquinone-based dyes exhibit optimal coplanarity for efficient electron transfer, with their LUMO and HOMO energy levels facilitating electron injection into TiO₂. In contrast to the low efficiencies found for previously studied anthraquinone derivatives, with maximum photocurrent efficiencies (PCE) below 0.2%, 9,10-anthraquinone derivatives were used as acceptors attached to suitable donors (DPA or Ind), yielding PCE above 1%.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"461 ","pages":"Article 116150"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic nanocomposite based on pyrene functionalized reduced graphene oxide in situ decorated with TiO2 nanorods","authors":"Chiara Ingrosso ,&nbsp;Alessia Disha ,&nbsp;Massimo Dell’Edera ,&nbsp;Ilaria De Pasquale ,&nbsp;Giuseppe V. Bianco ,&nbsp;Antonella Milella ,&nbsp;A. Grandolfo ,&nbsp;Ernesto Mesto ,&nbsp;Emanuela Schingaro ,&nbsp;Marinella Striccoli ,&nbsp;Elisabetta Fanizza ,&nbsp;Roberto Comparelli ,&nbsp;M. Lucia Curri","doi":"10.1016/j.jphotochem.2024.116149","DOIUrl":"10.1016/j.jphotochem.2024.116149","url":null,"abstract":"<div><div>Hybrid nanocomposites based on 1-pyrene carboxylic acid (PCA) functionalized High Porous Reduced Graphene Oxide (HPRGO) sheets, decorated with oleic acid (OLEA)-coated TiO₂ nanocrystals (NCs), have been obtained by means of an <em>in situ</em> colloidal route, starting from titanium isopropoxide (TTIP) precursor, in presence of OLEA surfactant and trimethylamino-N-oxide dihydrate (TMAO) base catalyst. The effect of the synthesis parameters, namely the PCA-HPRGO:TTIP w/w and the OLEA:TTIP molar ratio, on the morphological, spectroscopic and structural properties of the nanocomposites, has been explored, to achieve highly crystalline TiO₂ nanostructures, with a reproducible control on morphology and crystalline phase (anatase). The TiO₂ NCs have been found to effectively heteronucleate and grow onto the –COOH groups of the PCA molecules anchoring onto the HPRGO basal plane by aromatic π-π stacking interactions. The OLEA ligand coordinating their surface endows the nanocomposites with dispersibility in organic solvents, with a morphology dictated by the PCA coordinating sites, OLEA, and the mode of the TMAO supply to the reaction mixture. A significantly higher coating density has been found for the TiO₂ in nanorods (NRs) morphology, which organize in a uniform and high packed layout onto the PCA-HPRGO basal plane. The TiO₂ NRs decorated PCA-HPRGO nanocomposites (TiO₂ NRs/PCA-HPRGO) have been tested as photocatalysts for the degradation of methyl red (MR) and nalidixic acid (NA) under UV- and solar-light irradiation. Their photocatalytic activity has been evaluated against TiO₂ reference and commercial nanostructures and discussed in terms of electronic level alignment between the hybrid nanostructure components, considering the role of the PCA anchoring molecule at the interphase.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116149"},"PeriodicalIF":4.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel benzophenone derivatives photoinitiators based on carbazole group for deep curing and fast 3D printing","authors":"Shining Li ,&nbsp;Guanhao Ma ,&nbsp;Jinqing Qu","doi":"10.1016/j.jphotochem.2024.116144","DOIUrl":"10.1016/j.jphotochem.2024.116144","url":null,"abstract":"<div><div>With the development of photopolymerization in thick film curing, the design and synthesis of effective photoinitiators are becoming increasingly critical. A series of photoinitiators (4-(dimethylamino)phenyl)(9-ethyl-9H-carbazol-3-yl)methanone (PECM), (4-(dimethylamino)phenyl)(9-phenyl-9H-carbazol-3-yl)methanone (PPCM), (9-allyl-9H-carbazol-3-yl)(4-(dimethylamino)phenyl)methanone (PACM), and (4-(dimethylamino)phenyl)(9-(hex-5-en-1-yl)-9H-carbazol-3-yl)methanone (PHCM) based on carbazole group were synthesized for thick film curing. These photoinitiators exhibited the maximum absorption wavelength red-shifted to 353 nm compared to BP and had high molar absorption coefficients (over 27,000 L·mol⁻¹·cm⁻¹). Under the irradiation of LED, the three-component system composed of photoinitiator, diphenyliodonium hexafluorophosphate, and triethanolamine could initiate the rapid photopolymerization of polyethylene glycol diacrylate (PEGDA 400). Under low intensity LED@405 nm (5 mW/cm²) irradiation, PHCM achieved a final double bond conversion (DC) of 98 % within 20 s, significantly higher than BP (65 %). Under the irradiation of LED@365 nm (5 mW/cm²), PHCM reached 99 % DC within 8 s, while BP reached 97 % within 20 s. This performance was attributed to the fact that the molar absorption coefficients of PHCM at 365 nm (30010 L·mol⁻¹·cm⁻¹) and 405 nm (746 L·mol⁻¹·cm⁻¹) were much higher than those of BP (71 L·mol⁻¹·cm⁻¹, 46 L·mol⁻¹·cm⁻¹). Additionally, curing depth of PHCM reached 8.4 cm after 100 s irradiation under LED@405 nm. PHCM also demonstrated excellent cell compatibility and migration ratio as low as 1.8 %, which was 1/5 of that of BP (9.5 %). Furthermore, PHCM was suitable for 3D printers with 4 s/layer. In addition, the initiation mechanism of the photoinitiator was studied by photodegradation and ESR tests.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116144"},"PeriodicalIF":4.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced the efficiency of carbon based perovskite solar cells via g-C3N4 as functional additives","authors":"Xiaojie Yang ,&nbsp;Xiandong Zhao ,&nbsp;Li Zhao ,&nbsp;Shimin Wang","doi":"10.1016/j.jphotochem.2024.116145","DOIUrl":"10.1016/j.jphotochem.2024.116145","url":null,"abstract":"<div><div>The quality of perovskite layer plays an important role in the performance of perovskite solar cells. Various defects can arise during the crystal growth process of Perovskite thin film. However, by using additives. It is possible to effectively enhance crystallize and form high-quality perovskite films with improved morphology. g-C₃N₄, a carbon–nitrogen ring with delocalized π electrons and certain conductivity, facilitates the transport of charge carriers. When it is utilized as an additive in perovskite solar cells, g-C₃N₄ not only improves the quality and crystallinity of perovskite films, but also passivates defects. As a result of these improvements, the optimal efficiency for preparing carbon-based perovskite solar cells is 13.74 %. This represents a significant increase in photoelectric conversion efficiency from 10.39 % to 13.74 %, corresponding to a remarkable enhancement of 32.24 %. These findings provide valuable insights into the potential application of two-dimensional materials and carbon nitrogen ring for enhancing the performance of perovskite solar cells.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"461 ","pages":"Article 116145"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bright Blue-Light emitting cobalt doped CuS quantum dots: Photophysical studies and selective sensing application of ferric ion","authors":"Mitul Kalita,&nbsp;Mahabul Haque,&nbsp;Amarjyoti Mondal,&nbsp;Atanu Singha Roy","doi":"10.1016/j.jphotochem.2024.116137","DOIUrl":"10.1016/j.jphotochem.2024.116137","url":null,"abstract":"<div><div>Surface-functionalized quantum dots (QDs) have garnered significant attention in recent years for a variety of applications, including LEDs, photovoltaics, sensing, bioimaging and biomedical domains due to their distinct optical features such as strong photoluminescence behavior, high quantum yields etc. However, formation of nontoxic QDs is very challenging for the researchers because of their lower optical properties as compared to Cd based QDs. The phenomenon of doping in semiconductor QDs is an effective way to achieve high opto-electrical properties in the host QDs. We have presented a low temperature colloidal synthesis of CuS QDs using cobalt (Co²⁺) as a doping agent with an exceptional stability at room temperature for 30 days. The photoluminescence (PL) properties of Co²⁺-doped CuS QDs exhibit a deep-blue emission at 420 nm resulting in excellent optical property with an improvement of photoluminescence quantum yield. Due to remarkable CIE chromaticity coordinates, good CCT values, and high colour purity, the synthesized Co²⁺-doped CuS QDs could be used extensively in LEDs and prove to be useful as blue phosphors. The synthesized Co²⁺-doped CuS QDs also act as a fluorescence probe in the detection of ferric ion (Fe³⁺) with high sensitivity, good selectivity, a low limit of detection (LOD) and limit of quantification (LOQ), of (4.99 ± 0.12) μM and (16.67 ± 0.40) μM, respectively.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116137"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of glycerol oxidation on bismuth vanadate photoanodes: Influence of tantalum doping","authors":"Hitoshi Kusama,&nbsp;Kazuhiro Sayama","doi":"10.1016/j.jphotochem.2024.116143","DOIUrl":"10.1016/j.jphotochem.2024.116143","url":null,"abstract":"<div><div>This study investigated the mechanisms underlying the photoelectrochemical oxidation of glycerol to dihydroxyacetone (DHA) and glyceraldehyde (GLAD) on BiVO₄ and Bi–O–Ta species using density functional theory calculations. The results revealed that for both products and metal-oxides, the first proton-coupled electron-transfer (PCET) step, which forms a carbon-centered radical, served as the rate-limiting step. The formation of DHA from a secondary-carbon-centered radical encountered lower energy barriers compared to the formation of GLAD from a primary-carbon-centered radical, indicating the favorability of the DHA production pathway. The differences between the energy inputs required for DHA and GLAD production were greater on the Bi–O–Ta species compared to the BiVO₄. Furthermore, the production of formic acid (FA) from glycerol via GLAD, involving three H₂O molecules and eight PCET steps, encountered greater energy barriers on Bi–O–Ta species than on BiVO₄. These computational results confirm that the Bi–O–Ta species enhances DHA generation from glycerol while suppressing the production of unfavorable byproducts such as GLAD and FA. Correspondingly, the Ta-doped BiVO₄ photoanode achieves a greater Faradaic efficiency than the BiVO₄ photoanode and approximately 100% selectivity for DHA in acidic media owing to the Bi–O–Ta species formed on the surface.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116143"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant capacity of edaravone, quercetin, and myricetin involving probabilistic fluctuations using eosin-Y and eosin-B as fluorescent probes in the ORAC assay","authors":"Miwa Takatsuka ,&nbsp;Satoru Goto ,&nbsp;Kota Moritake ,&nbsp;Yosuke Shimada ,&nbsp;Tomohiro Tsuchida","doi":"10.1016/j.jphotochem.2024.116142","DOIUrl":"10.1016/j.jphotochem.2024.116142","url":null,"abstract":"<div><div>The oxygen radical absorbance capacity (ORAC) assay measures the antioxidant activity of the antioxidants through competitive consumption of peroxyl radicals relative to a fluorescent probe. This study evaluated fluorescein (FLH), eosin Y, and eosin B in ORAC assays using quantum computations. In ORAC-FLH assays, trolox (TRO) and ascorbic acid (ASC) showed fluorescence decay kinetics with a lag time, blocking initial reactions in the quenching cascade. Eosins were more reactive towards peroxyl radicals, making TRO assessment infeasible in ORAC-eosin assays. Quercetin and myricetin exhibited sigmoidal curve drifts proportional to squared and 3/2-ordered incubation times, indicating probabilistic fluorescence decay. Edaravone (EDA) weakly inhibited initial reactions, with accelerated quenching over time. In ORAC-eosin assays, flavonoids and EDA showed indistinguishable behavior due to high eosin reactivity. ORAC-FLH did not significantly assess BHT, though a dose-dependent change in half-life was noted. This study suggests broad applications for ORAC-eosin assays and potential for future comparative research.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116142"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}