Journal of physiology and biochemistry最新文献_第8页

Obituary Prof. Ana María Barber. 讣告 Ana María Barber 教授。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-09 DOI: 10.1007/s13105-024-01025-y

M Pilar Lostao

引用次数: 0

Novel insight on IRE1 in the regulation of chondrocyte dedifferentiation through ER stress independent pathway. 关于 IRE1 通过独立于 ER 应激的途径调控软骨细胞再分化的新见解。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-02-10 DOI: 10.1007/s13105-024-01008-z

Young Seok Eom, Fahad Hassan Shah, Song Ja Kim

{"title":"Novel insight on IRE1 in the regulation of chondrocyte dedifferentiation through ER stress independent pathway.","authors":"Young Seok Eom, Fahad Hassan Shah, Song Ja Kim","doi":"10.1007/s13105-024-01008-z","DOIUrl":"10.1007/s13105-024-01008-z","url":null,"abstract":"Inositol-requiring enzyme-1 (IRE1) is the master regulator of the unfolded protein response pathway, associated with the endoplasmic reticulum (ER) in sensing and regulating cell stress. The activity of IRE1 is highly explored and well-characterized in cancer and other cells. However, the IRE1 molecular mechanism in chondrocytes is poorly understood. The present study explored the effect of IRE1 on chondrocytes regarding its chondrogenic gene expression and its correlation with different cellular pathways and cell behavior. Chondrocytes transfected with the cDNA of IRE1 reduced the expression of type II collagen, disrupting chondrocyte differentiation as confirmed by western blotting and immunofluorescence. Upon siRNA treatment, the influence of IRE1 on chondrocyte differentiation is restored by reviving the normal expression of type II collagen. Different molecular pathways were explored to investigate the role of IRE1 in causing chondrocyte dedifferentiation. However, we found no significant correlation, as IRE1 induces dedifferentiation through independent pathways. In response to various endoplasmic reticulum (ER) agonists (2-deoxy-D-glucose), and ER stress antagonists (tauroursodeoxycholic acid and salubrinal), IRE1 overexpression did not affect GRP78/94, as implicated in the pathogenesis of ER stress. Moreover, when IRE1 overexpression was correlated with the inflammation pathway, nuclear factor-kappa B (NFκB), IRE1 substantially increased the expression of p50 while decreasing the expression of nuclear factor kappa light polypeptide alpha (IκBα). These results suggest that IRE1 induces dedifferentiation in chondrocytes by modulating inflammatory pathways that cause dedifferentiation by disrupting type II collagen expression.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"337-347"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual dimorphism in thermogenic regulators and metrnl expression in adipose tissue of offspring mice exposed to maternal and postnatal overnutrition. 受母体和出生后营养过剩影响的后代小鼠脂肪组织中致热调节因子和 metrnl 表达的性别双态性。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-16 DOI: 10.1007/s13105-024-01013-2

Zhao Yang, Jianan Jiang, Yutian Tan, Guiying Yang, Miao Chen, Jiaqi Huang, Jing Liu, Xiaojing Wei, Siyao Wang, Xiao Luo, Zhen Han

{"title":"Sexual dimorphism in thermogenic regulators and metrnl expression in adipose tissue of offspring mice exposed to maternal and postnatal overnutrition.","authors":"Zhao Yang, Jianan Jiang, Yutian Tan, Guiying Yang, Miao Chen, Jiaqi Huang, Jing Liu, Xiaojing Wei, Siyao Wang, Xiao Luo, Zhen Han","doi":"10.1007/s13105-024-01013-2","DOIUrl":"10.1007/s13105-024-01013-2","url":null,"abstract":"Current study investigated the impact of maternal and postnatal overnutrition on phenotype of adipose, in relation to offspring thermogenesis and sex. Female C57BL/6 J mice were fed with CHOW or high fat diet (HFD) for 2 weeks before mating, throughout gestation and lactation. At weaning, pups were fed to 9 weeks old with CHOW or HFD, which resulted in four groups for each gender--male or female: CHOW-CHOW (CC), CHOW-HFD (CH), HFD-CHOW (HC), HFD-HFD (HH). Maternal and post-weaning HFD enhanced thermogenic factors such as Acox1, Dio2 and Cox8b in iBAT of male and female offspring, but increased SIRT1, PGC-1α and UCP1 only in female. However, Acox1, Dio2 and Cox8b mRNA expression and SIRT1, PGC-1α and UCP1 protein expression were only enhanced upon maternal and post-weaning HFD in sWAT and pWAT of female offspring. Increased metrnl expression in adipose were observed in sex- and depot-specific manner, while enhanced circulating metrnl level was only observed in male offspring undergoing maternal HFD. Palmitic acid changed metrnl expression during preadipocytes differentiation and siRNA-mediated knockdown of metrnl inhibited preadipocyte differentiation. Female offspring were more prone to resist adverse outcomes induced by maternal and post-weaning overnutrition, which probably related to metrnl expression and thermogenesis.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"407-420"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-126 (MiR-126): key roles in related diseases. MicroRNA-126 (MiR-126)：在相关疾病中的关键作用。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-22 DOI: 10.1007/s13105-024-01017-y

Li Liao, Yan Tang, Yanping Zhou, Xianglin Meng, Bo Li, Xiaochun Zhang

引用次数: 0

Exercise-induced changes in plasma S-Klotho levels are associated with the obtained enhancements of heart rate variability in sedentary middle-aged adults: the FIT-AGEING study. 运动引起的血浆 S-Klotho 水平变化与久坐不动的中年人心率变异性的增强有关：FIT-AGEING 研究。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-01-04 DOI: 10.1007/s13105-023-01005-8

Ginés Navarro-Lomas, Abel Plaza-Florido, Alejandro De-la-O, Manuel J Castillo, Francisco J Amaro-Gahete

{"title":"Exercise-induced changes in plasma S-Klotho levels are associated with the obtained enhancements of heart rate variability in sedentary middle-aged adults: the FIT-AGEING study.","authors":"Ginés Navarro-Lomas, Abel Plaza-Florido, Alejandro De-la-O, Manuel J Castillo, Francisco J Amaro-Gahete","doi":"10.1007/s13105-023-01005-8","DOIUrl":"10.1007/s13105-023-01005-8","url":null,"abstract":"The shed form of the Klotho protein (S-Klotho) is considered a biomarker of longevity, but it is still unknown whether the levels are related to heart rate (HR) and heart rate variability (HRV); both of them greatly influenced by the ageing process, physical fitness, exercise, and health status. This study aimed (i) to investigate the association between S-Klotho plasma levels with HR and HRV parameters and (ii) to examine the association of exercise-induced changes in S-Klotho and those obtained in HR and HRV parameters after a 12-week exercise intervention in sedentary middle-aged adults. Sixty-six sedentary middle-aged adults participated in this study (50% women; 45-65 years old). Participants were randomized into 4 groups: (a) a control group (no exercise), (b) a physical activity recommendation from the World Health Organization group, (c) a high-intensity interval training group, and (d) a high-intensity interval training group adding whole-body electromyostimulation. S-Klotho plasma levels, HR, and HRV parameters (SDNN, RMSSD, high frequency, stress score, and sympathetic/parasympathetic ratio) were measured. At baseline, S-Klotho plasma levels were not related to HR and HRV parameters. After the intervention, exercise-induced changes in S-Klotho plasma levels were positively associated with changes in SDNN (β=0.261; R2=0.102; p=0.014) and negatively related to changes in stress score and sympathetic/parasympathetic ratio (all β=-0.257; R2 ranges between 0.092 and 0.131; all p<0.020). Our study suggests that higher S-Klotho plasma levels are related to increased vagal influence and reduced sympathetic tone in the autonomic nervous system in sedentary middle-aged adults after different training programs. ClinicalTrials.gov identifier: CT03334357.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"317-328"},"PeriodicalIF":3.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12132908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139087318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle. PGC-1α 激活可促进骨骼肌中依赖运动的细胞反应。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-01-23 DOI: 10.1007/s13105-024-01006-1

Soroosh Mozaffaritabar, Erika Koltai, Lei Zhou, Zoltan Bori, Attila Kolonics, Sylwester Kujach, Yaodong Gu, Atsuko Koike, Anita Boros, Zsolt Radák

{"title":"PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle.","authors":"Soroosh Mozaffaritabar, Erika Koltai, Lei Zhou, Zoltan Bori, Attila Kolonics, Sylwester Kujach, Yaodong Gu, Atsuko Koike, Anita Boros, Zsolt Radák","doi":"10.1007/s13105-024-01006-1","DOIUrl":"10.1007/s13105-024-01006-1","url":null,"abstract":"The role of Peroxisome proliferator-activated receptor-gamma coactivator alpha (PGC-1α) in fat metabolism is not well known. In this study, we compared the mechanisms of muscle-specific PGC-1α overexpression and exercise-related adaptation-dependent fat metabolism. PGC-1α trained (PGC-1α Ex) and wild-trained (wt-ex) mice were trained for 10 weeks, five times a week at 30 min per day with 60 percent of their maximal running capacity. The PGC-1α overexpressed animals exhibited higher levels of Fibronectin type III domain-containing protein 5 (FNDC5), 5' adenosine monophosphate-activated protein kinase alpha (AMPK-α), the mammalian target of rapamycin (mTOR), Sirtuin 1 (SIRT1), Lon protease homolog 1 (LONP1), citrate synthase (CS), succinate dehydrogenase complex flavoprotein subunit A (SDHA), Mitofusin-1 (Mfn1), endothelial nitric oxide synthase (eNOS), Hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), G protein-coupled receptor 41 (GPR41), and Phosphatidylcholine Cytidylyltransferase 2 (PCYT2), and lower levels of Sirtuin 3 (SIRT3) compared to wild-type animals. Exercise training increased the protein content levels of SIRT1, HSL, and ATGL in both the wt-ex and PGC-1α trained groups. PGC-1α has a complex role in cellular signaling, including the upregulation of lipid metabolism-associated proteins. Our data reveals that although exercise training mimics the effects of PGC-1α overexpression, it incorporates some PGC-1α-independent adaptive mechanisms in fat uptake and cell signaling.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"329-335"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139521270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sorcin promotes proliferation of hepatocellular carcinoma by regulating VEGFA/B via PI3K pathway. Sorcin通过PI3K途径调节VEGFA/B，促进肝细胞癌的增殖。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-27 DOI: 10.1007/s13105-024-01011-4

Huan Zhang, Shanshan Hu, Jaceline Gislaine Pires Sanches, Yizi Li, Yuanyi Wei, Chunwen Pu, Jun Zhang

引用次数: 0

The role of PKM2 in cancer progression and its structural and biological basis. PKM2 在癌症进展中的作用及其结构和生物学基础。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-02-08 DOI: 10.1007/s13105-024-01007-0

Bingxin Wu, Zuhui Liang, Huan Lan, Xiaojun Teng, Caiyan Wang

{"title":"The role of PKM2 in cancer progression and its structural and biological basis.","authors":"Bingxin Wu, Zuhui Liang, Huan Lan, Xiaojun Teng, Caiyan Wang","doi":"10.1007/s13105-024-01007-0","DOIUrl":"10.1007/s13105-024-01007-0","url":null,"abstract":"Pyruvate kinase M2 (PKM2), a subtype of pyruvate kinase (PK), has been shown to play an important role in the development of cancer. It regulates the last step of glycolytic pathway. PKM2 has both pyruvate kinase and protein kinase activity, and the conversion of these two functions of PKM2 depends on the mutual change of dimer and tetramer. The dimerization of PKM2 can promote the proliferation and growth of tumor cells, so inhibiting the dimerization of PKM2 is essential to curing cancer. The aggregation of PKM2 is regulated by both endogenous and exogenous cofactors as well as post-translational modification (PTM). Although there are many studies on the different aggregation of PKM2 in the process of tumor development, there are few summaries in recent years. In this review, we first introduce the role of PKM2 in various biological processes of tumor growth. Then, we summarize the aggregation regulation mechanism of PKM2 by various endogenous cofactors such as Fructose-1, 6-diphosphate (FBP), various amino acids, and post-translational modification (PTMs). Finally, the related inhibitors and agonists of PKM2 are summarized to provide reference for regulating PKM2 aggregation in the treatment of cancer in the future.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"261-275"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139702768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chronic cold stress on gut microbial diversity, intestinal inflammation and pyroptosis in mice. 慢性冷应激对小鼠肠道微生物多样性、肠道炎症和热变态反应的影响

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-25 DOI: 10.1007/s13105-024-01019-w

Hongming Lv, Shijie Xia, Yuxi He, Chunyu Qiao, Jiahe Liu, Jingru Guo, Shize Li

{"title":"Effect of chronic cold stress on gut microbial diversity, intestinal inflammation and pyroptosis in mice.","authors":"Hongming Lv, Shijie Xia, Yuxi He, Chunyu Qiao, Jiahe Liu, Jingru Guo, Shize Li","doi":"10.1007/s13105-024-01019-w","DOIUrl":"10.1007/s13105-024-01019-w","url":null,"abstract":"Hypothermia is an essential environmental factor in gastrointestinal diseases, but the main molecular mechanisms of pathogenesis remain unclear. The current study sought to better understand how chronic cold stress affects gut damage and its underlying mechanisms. In this work, to establish chronic cold stress (CS)-induced intestinal injury model, mice were subjected to continuous cold exposure (4 °C) for 3 h per day for 3 weeks. Our results indicated that CS led to gut injury via inducing changes of heat shock proteins 70 (HSP70) and apoptosis-related (caspases-3, Bax and Bcl-2) proteins; enhancing expression of intestinal tight-related (ZO-1 and occludin) proteins; promoting releases of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), high mobility group box 1 (HMGB1), interleukin1β (IL-1β), IL-18 and IL-6 inflammatory mediators in the ileum; and altering gut microbial diversity. Furthermore, persistent cold exposure resulted in the cleavage of pyroptosis-related Gasdermin D (GSDMD) protein by regulating the NLRP3/ASC/caspase-1 and caspase-11 pathway, and activation of toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)-mediated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, which are strongly associated with changes in gut microbiota diversity. Taken together, these investigations provide new insights into the increased risk of intestinal disorders at extremely low temperatures and establish a theoretical foundation for the advancement of novel pharmaceutical interventions targeting cold-related ailments.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"465-477"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactate ameliorates palmitate-induced impairment of differentiative capacity in C2C12 cells through the activation of voltage-gated calcium channels. 乳酸盐通过激活电压门控钙通道改善棕榈酸酯诱导的 C2C12 细胞分化能力损伤。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1007/s13105-024-01009-y

Juan Wan, Chunfang Cheng, Xiaonuo Li, Yuanjie Zhu, Hu Su, Yanchun Gong, Kaizhi Ding, Xiaofei Gao, Caixia Dang, Guoyin Li, Wei Jiang, Li-Hua Yao

{"title":"Lactate ameliorates palmitate-induced impairment of differentiative capacity in C2C12 cells through the activation of voltage-gated calcium channels.","authors":"Juan Wan, Chunfang Cheng, Xiaonuo Li, Yuanjie Zhu, Hu Su, Yanchun Gong, Kaizhi Ding, Xiaofei Gao, Caixia Dang, Guoyin Li, Wei Jiang, Li-Hua Yao","doi":"10.1007/s13105-024-01009-y","DOIUrl":"10.1007/s13105-024-01009-y","url":null,"abstract":"Palmitic acid (PA), a saturated fatty acid enriched in high-fat diet, has been implicated in the development of skeletal muscle regeneration dysfunction. This study aimed to examine the effects and mechanisms of lactate (Lac) treatment on PA-induced impairment of C2C12 cell differentiation capacity. Furthermore, the involvement of voltage-gated calcium channels in this context was examined. In this study, Lac could improve the PA-induced impairment of differentiative capacity in C2C12 cells by affecting Myf5, MyoD and MyoG. In addition, Lac increases the inward flow of Ca2+, and promotes the depolarization of the cell membrane potential, thereby activating voltage-gated calcium channels during C2C12 cell differentiation. The enchancement of Lac on myoblast differentiative capacity was abolished after the addition of efonidipine (voltage-gated calcium channel inhibitors). Therefore, voltage-gated calcium channels play an important role in improving PA-induced skeletal muscle regeneration disorders by exercising blood Lac. Our study showed that Lac could rescue the PA-induced impairment of differentiative capacity in C2C12 cells by affecting Myf5, MyoD and MyoG through the activation of voltage-gated calcium channels.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"349-362"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0