Journal of physiology and biochemistry最新文献_第8页

Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1. 弹性蛋白衍生肽诱导的序列酸裂解会损害 3T3-L1 脂肪细胞中胰岛素与其受体之间的相互作用。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-02-23 DOI: 10.1007/s13105-024-01010-5

Alexandre Guillot, Kevin Toussaint, Lucrece Ebersold, Hassan ElBtaouri, Emilie Thiebault, Tarik Issad, Franck Peiretti, Pascal Maurice, Hervé Sartelet, Amar Bennasroune, Laurent Martiny, Manuel Dauchez, Laurent Duca, Vincent Durlach, Béatrice Romier, Stéphanie Baud, Sébastien Blaise

{"title":"Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1.","authors":"Alexandre Guillot, Kevin Toussaint, Lucrece Ebersold, Hassan ElBtaouri, Emilie Thiebault, Tarik Issad, Franck Peiretti, Pascal Maurice, Hervé Sartelet, Amar Bennasroune, Laurent Martiny, Manuel Dauchez, Laurent Duca, Vincent Durlach, Béatrice Romier, Stéphanie Baud, Sébastien Blaise","doi":"10.1007/s13105-024-01010-5","DOIUrl":"10.1007/s13105-024-01010-5","url":null,"abstract":"The insulin receptor (IR) plays an important role in insulin signal transduction, the defect of which is believed to be the root cause of type 2 diabetes. In 3T3-L1 adipocytes as in other cell types, the mature IR is a heterotetrameric cell surface glycoprotein composed of two α subunits and two β subunits. Our objective in our study, is to understand how the desialylation of N-glycan chains, induced by elastin-derived peptides, plays a major role in the function of the IR. Using the 3T3-L1 adipocyte line, we show that removal of the sialic acid from N-glycan chains (N893 and N908), induced by the elastin receptor complex (ERC) and elastin derived-peptides (EDPs), leads to a decrease in the autophosphorylation activity of the insulin receptor. We demonstrate by molecular dynamics approaches that the absence of sialic acids on one of these two sites is sufficient to generate local and general modifications of the structure of the IR. Biochemical approaches highlight a decrease in the interaction between insulin and its receptor when ERC sialidase activity is induced by EDPs. Therefore, desialylation by EDPs is synonymous with a decrease of IR sensitivity in adipocytes and could thus be a potential source of insulin resistance associated with diabetic conditions.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"363-379"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139931606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of microRNA-363 during tumor progression and invasion microRNA-363 在肿瘤进展和侵袭过程中的作用

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 DOI: 10.1007/s13105-024-01022-1

Arya Nasimi Shad, Iman Akhlaghipour, Hawraa Ibrahim Alshakarchi, Ehsan Saburi, Meysam Moghbeli

{"title":"Role of microRNA-363 during tumor progression and invasion","authors":"Arya Nasimi Shad, Iman Akhlaghipour, Hawraa Ibrahim Alshakarchi, Ehsan Saburi, Meysam Moghbeli","doi":"10.1007/s13105-024-01022-1","DOIUrl":"https://doi.org/10.1007/s13105-024-01022-1","url":null,"abstract":"Recent progresses in diagnostic and therapeutic methods have significantly improved prognosis in cancer patients. However, cancer is still considered as one of the main causes of human deaths in the world. Late diagnosis in advanced tumor stages can reduce the effectiveness of treatment methods and increase mortality rate of cancer patients. Therefore, investigating the molecular mechanisms of tumor progression can help to introduce the early diagnostic markers in these patients. MicroRNA (miRNAs) has an important role in regulation of pathophysiological cellular processes. Due to their high stability in body fluids, they are always used as the non-invasive markers in cancer patients. Since, miR-363 deregulation has been reported in a wide range of cancers, we discussed the role of miR-363 during tumor progression and metastasis. It has been reported that miR-363 has mainly a tumor suppressor function through the regulation of transcription factors, apoptosis, cell cycle, and structural proteins. MiR-363 also affected the tumor progression via regulation of various signaling pathways such as WNT, MAPK, TGF-β, NOTCH, and PI3K/AKT. Therefore, miR-363 can be introduced as a probable therapeutic target as well as a non-invasive diagnostic marker in cancer patients.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"3 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in extracellular vesicle homeostasis as it relates to cardiovascular diseases 细胞外囊泡稳态与心血管疾病的关系研究进展

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-30 DOI: 10.1007/s13105-024-01027-w

Yunan Kang, Wenqian Wu, Yi Yang, Jinxi Luo, Yajie Lu, Luchang Yin, Xiaodong Cui

{"title":"Progress in extracellular vesicle homeostasis as it relates to cardiovascular diseases","authors":"Yunan Kang, Wenqian Wu, Yi Yang, Jinxi Luo, Yajie Lu, Luchang Yin, Xiaodong Cui","doi":"10.1007/s13105-024-01027-w","DOIUrl":"https://doi.org/10.1007/s13105-024-01027-w","url":null,"abstract":"Extracellular vesicles (EVs) are involved in both physiological and pathological processes in many organ systems and are essential in mediating intercellular communication and maintaining organismal homeostasis. It is helpful to propose new strategies for disease treatment by elucidating the mechanisms of EV release and sorting. An increasing number of studies have shown that there is specific homeostasis in EVs, which is helpful for the human body to carry out physiological activities. In contrast, an EV homeostasis im-balance promotes or accelerates disease onset and development. Alternatively, regulating the quality of EVs can maintain homeostasis and even achieve the purpose of treating conditions. An analysis of the role of EV homeostasis in the onset and development of cardiovascular disease is presented in this review. This article also summarizes the methods that regulate EV homeostasis and their application in cardiovascular diseases. In particular, this study focuses on the connection between EV steady states and the cardiovascular system and the potential value of EVs in treating cardiovascular diseases.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"23 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovalbumin promotes innate immune response of Caenorhabditis elegans through DAF-16 and SKN-1 pathways in insulin/IGF-1 signaling 卵清蛋白通过胰岛素/IGF-1 信号转导中的 DAF-16 和 SKN-1 通路促进秀丽隐杆线虫的先天性免疫反应

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-18 DOI: 10.1007/s13105-024-01021-2

Haibing Pei, Zhiyin Lin, Kexin Yao, Yeqing Luo, Ping Tong, Hongbing Chen, Yong Wu, Zhihua Wu, Jinyan Gao

{"title":"Ovalbumin promotes innate immune response of Caenorhabditis elegans through DAF-16 and SKN-1 pathways in insulin/IGF-1 signaling","authors":"Haibing Pei, Zhiyin Lin, Kexin Yao, Yeqing Luo, Ping Tong, Hongbing Chen, Yong Wu, Zhihua Wu, Jinyan Gao","doi":"10.1007/s13105-024-01021-2","DOIUrl":"https://doi.org/10.1007/s13105-024-01021-2","url":null,"abstract":"Ovalbumin (OVA) is a major allergen in eggs and could induce severe allergic reactions in sensitive individuals, where the innate immune system works as a regulator. The mechanism of how innate immunity adjusts to food allergy is relatively well-studied, however, the effects of allergen uptake on the innate immune system remain unclear. Therefore, the Caenorhabditis elegans (C. elegans) model was utilized to assess the effects of OVA on its innate immune system. OVA enhanced the immune response of C. elegans with higher survival rates under Pseudomonas aeruginosa infection. Moreover, sustaining OVA treatment improved the health states that were reflected in the prolonged lifespan, alleviated oxidative stress, accelerated growth, and promoted motility. RNA-sequencing analysis and the slow-killing assays in the mutants of insulin/IGF-1 signaling (IIS)-related genes confirmed that IIS was necessary for OVA to regulate innate immunity. Besides, OVA activated SKN-1 temporarily and facilitated the nuclear localization of DAF-16 for improving immunity and health status in C. elegans. Together, OVA could enhance the innate immune responses via DAF-16 and SKN-1 pathways in the IIS of C. elegans, and this work will provide novel insights into the regulation of innate immunity by OVA in higher organisms.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"12 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fasting and fasting-mimicking conditions in the cancer immunotherapy era 癌症免疫疗法时代的禁食和模拟禁食条件

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-08 DOI: 10.1007/s13105-024-01020-3

Ruben Pio, Yaiza Senent, Beatriz Tavira, Daniel Ajona

引用次数: 0

Naturally occurring small molecules with dual effect upon inflammatory signaling pathways and endoplasmic reticulum stress response 对炎症信号通路和内质网应激反应具有双重作用的天然小分子

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-03-19 DOI: 10.1007/s13105-024-01014-1

Daniela Correia da Silva, Patrícia Valentão, David M. Pereira

{"title":"Naturally occurring small molecules with dual effect upon inflammatory signaling pathways and endoplasmic reticulum stress response","authors":"Daniela Correia da Silva, Patrícia Valentão, David M. Pereira","doi":"10.1007/s13105-024-01014-1","DOIUrl":"https://doi.org/10.1007/s13105-024-01014-1","url":null,"abstract":"The endoplasmic reticulum (ER) is determinant to maintain cellular proteostasis. Upon unresolved ER stress, this organelle activates the unfolded protein response (UPR). Sustained UPR activates is known to occur in inflammatory processes, deeming the ER a potential molecular target for the treatment of inflammation. This work characterizes the inflammatory/UPR-related molecular machinery modulated by an in-house library of natural products, aiming to pave the way for the development of new selective drugs that act upon the ER to counter inflammation-related chronic diseases. Starting from a library of 134 compounds of natural occurrence, mostly occurring in medicinal plants, nontoxic molecules were screened for their inhibitory capacity against LPS-induced nuclear factor kappa B (NF-κB) activation in a luciferase-based reporter gene assay. Since several natural products inhibited NF-κB expression in THP-1 macrophages, their effect on reactive oxygen species (ROS) production and inflammasome activation was assessed, as well as their transcriptional outcome regarding ER stress. The bioactivities of several natural products are described herein for the first time. We report the anti-inflammatory potential of guaiazulene and describe 5-deoxykaempferol as a novel inhibitor of inflammasome activation. Furthermore, we describe the dual potential of 5-deoxykaempferol, berberine, guaiazulene, luteolin-4’-O-glucoside, myricetin, quercetagetin and sennoside B to modulate inflammatory signaling ER stress. Our results show that natural products are promising molecules for the discovery and pharmaceutical development of chemical entities able to modulate the inflammatory response, as well as proteostasis and the UPR.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of the vagus nerve and hepatic gene expression in serum adiponectin concentrations in mice. 迷走神经和肝脏基因表达对小鼠血清脂联素浓度的影响。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-02-01 Epub Date: 2023-10-14 DOI: 10.1007/s13105-023-00987-9

Naoto Hashimoto, Ryuji Nagata, Kyu-Ho Han, Manabu Wakagi, Yuko Ishikawa-Takano, Michihiro Fukushima

{"title":"Involvement of the vagus nerve and hepatic gene expression in serum adiponectin concentrations in mice.","authors":"Naoto Hashimoto, Ryuji Nagata, Kyu-Ho Han, Manabu Wakagi, Yuko Ishikawa-Takano, Michihiro Fukushima","doi":"10.1007/s13105-023-00987-9","DOIUrl":"10.1007/s13105-023-00987-9","url":null,"abstract":"Several humoral factors, such as adiponectin and urate, have been suggested to affect metabolic syndromes. Previously, we reported a reduction in blood adiponectin concentrations after a high-fructose diet partially via the vagus nerve in rats. Although a lithogenic diet (LD), i.e., supplementation of a normal control diet (CT) with 0.6% cholesterol and 0.2% sodium cholate, reduced blood adiponectin concentrations, the involvement of the vagus nerve in this mechanism remains unclear. To estimate the involvement of the vagus nerve in the regulation of blood adiponectin concentrations using an LD, male imprinting control region mice that had been vagotomized (HVx) or only laparotomized (Sham) were administered a CT or an LD for 10 weeks. Serum adiponectin concentrations in the Sham-LD, HVx-CT, and HVx-LD groups were reduced by half compared with the Sham-CT group. The hepatic mRNA levels of fibroblast growth factor 21 (Fgf21), which reportedly stimulates adiponectin secretion from white adipose tissue, were lower in the LD groups compared with the CT groups. HepG2 hepatoma cells showed that various bile acids reduced the mRNA expression of FGF21. Moreover, the LD increased serum urate concentrations and reduced hepatic expressions of the acyl-CoA oxidase 1 (Acox1) mRNA and glucokinase, suggesting insufficient regeneration of ATP from AMP. In conclusion, serum adiponectin concentration may be regulated via the vagus nerve in normal mice, whereas a reduction of hepatic Fgf21 mRNA by bile acids may also lower serum adiponectin levels. Moreover, the LD may promote hepatic AMP accumulation and subsequently increase the serum urate concentration in mice.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"99-112"},"PeriodicalIF":3.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parkin depletion prevents the age-related alterations in the FGF21 system and the decline in white adipose tissue thermogenic function in mice. Parkin耗竭可防止小鼠FGF21系统与年龄相关的改变和白色脂肪组织产热功能的下降。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-02-01 Epub Date: 2023-11-02 DOI: 10.1007/s13105-023-00977-x

Alejandro Delgado-Anglés, Albert Blasco-Roset, Francisco J Godoy-Nieto, Montserrat Cairó, Francesc Villarroya, Marta Giralt, Joan Villarroya

{"title":"Parkin depletion prevents the age-related alterations in the FGF21 system and the decline in white adipose tissue thermogenic function in mice.","authors":"Alejandro Delgado-Anglés, Albert Blasco-Roset, Francisco J Godoy-Nieto, Montserrat Cairó, Francesc Villarroya, Marta Giralt, Joan Villarroya","doi":"10.1007/s13105-023-00977-x","DOIUrl":"10.1007/s13105-023-00977-x","url":null,"abstract":"Parkin is an ubiquitin-E3 ligase that is involved in cellular mitophagy and was recently shown to contribute to controlling adipose tissue thermogenic plasticity. We found that Parkin expression is induced in brown (BAT) and white (WAT) adipose tissues of aged mice. We determined the potential role of Parkin in the aging-associated decline in the thermogenic capacity of adipose tissues by analyzing subcutaneous WAT, interscapular BAT, and systemic metabolic and physiological parameters in young (5 month-old) and aged (16 month-old) mice with targeted invalidation of the Parkin (Park2) gene, and their wild-type littermates. Our data indicate that suppression of Parkin prevented adipose accretion, increased energy expenditure and improved the systemic metabolic derangements, such as insulin resistance, seen in aged mice. This was associated with maintenance of browning and reduction of the age-associated induction of inflammation in subcutaneous WAT. BAT in aged mice was much less affected by Parkin gene invalidation. Such protection was associated with a dramatic prevention of the age-associated induction of fibroblast growth factor-21 (FGF21) levels in aged Parkin-invalidated mice. This was associated with a parallel reduction in FGF21 gene expression in adipose tissues and liver in aged Parkin-invalidated mice. Additionally, Parkin invalidation prevented the protein down-regulation of β-Klotho (a key co-receptor mediating FGF21 responsiveness in tissues) in aged adipose tissues. We conclude that Parkin down-regulation leads to improved systemic metabolism in aged mice, in association with maintenance of adipose tissue browning and FGF21 system functionality.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"41-51"},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10808413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71424480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing suppressor of cytokine signaling 3 induces apoptosis and activates the p-STAT3/NF-κB pathway in hypoxic cultivated H9c2 cells. 细胞因子信号转导沉默抑制剂3在缺氧培养的H9c2细胞中诱导细胞凋亡并激活p-STAT3/NF-κB通路。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-02-01 Epub Date: 2023-10-19 DOI: 10.1007/s13105-023-00989-7

Qiang Gu, Ying-Bin Xiao, Yong Wang

{"title":"Silencing suppressor of cytokine signaling 3 induces apoptosis and activates the p-STAT3/NF-κB pathway in hypoxic cultivated H9c2 cells.","authors":"Qiang Gu, Ying-Bin Xiao, Yong Wang","doi":"10.1007/s13105-023-00989-7","DOIUrl":"10.1007/s13105-023-00989-7","url":null,"abstract":"Suppressor of cytokine signaling 3 (SOCS3) plays a significant role in the process of myocardial adaptation to chronic hypoxia. SOCS3 finely regulates cell signaling cross-talk that occurs between NF-κB and STAT3 during the compensatory protective response. However, the role and mechanism of SOCS3 in hypoxic cardiomyocytes are not fully understood. In the study, we investigated the effect of SOCS3 on the p65 and STAT3 signaling pathways and further examined the potential molecular mechanism involved in regulating apoptosis. Our data showed that SOCS3 silencing could upregulate Ac-p65, p-p65, and p-STAT3 expression in nuclear extracts of H9c2 cells that received hypoxic treatment for 24, 48, and 72 h. SOCS3 silencing also remarkably increased the DNA-binding activity of the p65 motif in hypoxic cultivated H9c2 cells. We also found that SOCS3 knockdown increased cleaved-caspase-3, Bax, and PUMA expression and decreased cleaved PARP and Bcl-2 in expression in hypoxic H9c2 cells. Silencing of SOCS3 caused an increase in LDH leakage from injured cardiomyocytes and reduced cell viability under conditions of hypoxic stress. Furthermore, SOCS3 silencing enhanced the apoptosis of H9c2 cells at 72 h of hypoxia. These findings suggest that knockdown of SOCS3 leads to excessive activation of the NF-κB pathway, which, in turn, might promote apoptosis under conditions of chronic hypoxia.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"127-136"},"PeriodicalIF":3.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49678734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caveolin-1 deficiency alleviates palmitate-induced intracellular lipid accumulation and inflammation in pancreatic β cells. 小窝蛋白-1缺乏可减轻棕榈酸盐诱导的胰腺β细胞内脂质积累和炎症。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-02-01 Epub Date: 2023-11-30 DOI: 10.1007/s13105-023-00995-9

Wen Zeng, Nan Cai, Jia Liu, Kunying Liu, Shuo Lin, Longyi Zeng

{"title":"Caveolin-1 deficiency alleviates palmitate-induced intracellular lipid accumulation and inflammation in pancreatic β cells.","authors":"Wen Zeng, Nan Cai, Jia Liu, Kunying Liu, Shuo Lin, Longyi Zeng","doi":"10.1007/s13105-023-00995-9","DOIUrl":"10.1007/s13105-023-00995-9","url":null,"abstract":"Lipotoxicity-induced pancreatic β cell damage is a strong predictor of type 2 diabetes mellitus (T2DM). Our previous work showed that Caveolin-1 (Cav-1) depletion decreased β-cell apoptosis and improved β-cell viability. Further microarray analysis indicated significant changes in the expression of genes related to fatty acid metabolism and inflammation. The objective of this study was to explore the role of Cav-1 in intracellular lipid accumulation and inflammation in β cells under lipotoxic conditions. Here, we established a β-cell-specific Cav-1 knockout (β-Cav-1 KO) mouse model and a CAV-1 depleted β cell line (NIT-1). We found that Cav-1 silencing significantly reduced palmitate (PA)-induced intracellular triglyceride (TG) accumulation and decreased proinflammatory factor expression in both the mouse and cell models. Further mechanistic investigation revealed that amelioration of lipid metabolism was achieved through the downregulation of lipogenic markers (SREBP-1c, FAS and ACC) and upregulation of a fatty acid oxidation marker (CPT-1). Meanwhile, decrease of inflammatory cytokines (IL-6, TNF-α, and IL-1β) secretion was found with the involvement of the IKKβ/NF-κB signaling pathways. Our findings suggest that Cav-1 is of considerable importance in regulating lipotoxicity-induced β-cell intracellular lipid accumulation and inflammation.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"175-188"},"PeriodicalIF":3.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0