Journal of physiology and biochemistry最新文献_第9页

Itaconate inhibits corticosterone-induced necroptosis and neuroinflammation via up-regulating menin in HT22 cells. 伊塔康酸通过上调 HT22 细胞中的 menin 抑制皮质酮诱导的坏死和神经炎症。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-01 DOI: 10.1007/s13105-024-01012-3

Jin-Yu Liang, Shan Gao, Jia-Mei Jiang, Pin Zhang, Wei Zou, Xiao-Qing Tang, Yi-Yun Tang

{"title":"Itaconate inhibits corticosterone-induced necroptosis and neuroinflammation via up-regulating menin in HT22 cells.","authors":"Jin-Yu Liang, Shan Gao, Jia-Mei Jiang, Pin Zhang, Wei Zou, Xiao-Qing Tang, Yi-Yun Tang","doi":"10.1007/s13105-024-01012-3","DOIUrl":"10.1007/s13105-024-01012-3","url":null,"abstract":"Corticosterone (CORT) damages hippocampal neurons as well as induces neuroinflammation. The tricarboxylic acid cycle metabolite itaconate has an anti-inflammatory role. Necroptosis is a form of programmed cell death, also known as inflammatory cell death. Menin is a multifunctional scaffold protein, which deficiency aggravates neuroinflammation. In this study, we explored whether itaconate inhibits CORT-induced neuroinflammation as well as necroptosis and further investigated the mediatory role of Menin in this protective effect of itaconate by using an exposure of CORT to HT22 cells (a hippocampal neuronal cell line). The viability of HT22 cells was examined by the cell counting kit 8 (CCK-8). The morphology of HT22 cells was observed by transmission electron microscope (TEM). The expressions of necroptosis-related proteins (p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL) were evaluated by western blotting. The contents of inflammatory factors were detected by an enzyme-linked immunosorbent assay (ELISA) kit. Our results showed that CORT increases the contents of pro-inflammatory factors (IL-1β, TNF-α) as well as decreases the contents of anti-inflammatory factors (IL-4, IL-10) in HT22 cells. We also found that CORT increases the expressions of necroptosis-related proteins (p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL) and decreases the cell viability in HT22 cells, indicating that CORT induces necroptosis in HT22 cells. Itaconate improves CORT-induced neuroinflammation and necroptosis. Furthermore, itaconate upregulates the expression of Menin in CORT-exposed HT22 cells. Importantly, silencing Menin abolishes the antagonistic effect of itaconate on CORT-induced necroptosis and neuroinflammation. In brief, these results indicated that itaconate protects HT22 cells against CORT-induced neuroinflammation and necroptosis via upregulating Menin.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"393-405"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An experimental model of western diet in female Wistar rats leads to cardiac hypoxia related to a stimulated contractility. 雌性 Wistar 大鼠的西式饮食实验模型会导致心脏缺氧，这与刺激收缩力有关。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-01-04 DOI: 10.1007/s13105-023-01003-w

Jean-Paul Rigaudière, Chrystèle Jouve, Frédéric Capel, Véronique Patrac, Bruno Miguel, Anne Tournadre, Luc Demaison

{"title":"An experimental model of western diet in female Wistar rats leads to cardiac hypoxia related to a stimulated contractility.","authors":"Jean-Paul Rigaudière, Chrystèle Jouve, Frédéric Capel, Véronique Patrac, Bruno Miguel, Anne Tournadre, Luc Demaison","doi":"10.1007/s13105-023-01003-w","DOIUrl":"10.1007/s13105-023-01003-w","url":null,"abstract":"Previous studies in Western diet (WD)-fed male rats have highlighted a link between the stimulation of cardiac contractility, mitochondrial adaptations and a pro-inflammatory fatty acid profile of phospholipids in the heart. Our objectives were to determine (1) if WD-fed female Wistar rats and obese humans display a similar pro-inflammatory profile in their cardiac phospholipids and (2) if this lipid profile is associated with deleterious effects on the heart of the female rodents. Female Wistar rats were fed WD for 5 weeks or a laboratory chow as a control. Ionic homeostasis, redox status, inflammation markers, and fatty acid composition of phospholipids were analysed in the heart. WD increased the abdominal fat mass without modifying the body weight of female rats. As previously found in males, a WD induced a shift in membrane fatty acid composition toward a pro-inflammatory profile in the female rats, but not in obese humans. It was associated with an increased COX2 expression suggesting an increased pro-inflammatory eicosanoid production. Signs of increased intracellular calcium strongly supported a stimulation of cardiac contractility without any induction of apoptosis. The heart of WD-fed rats exhibited a hypoxic state as a higher HIF1-α expression was reported. The expressions of antioxidant enzymes were increased, but the redox reserves against reactive oxygen species were lowered. In conclusion, as previously observed in males, we suppose that cardiac abnormalities are magnified with severe obesity in female rats, leading to hypoxia and intense oxidative stress which could ultimately induce cell death and heart failure.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"287-302"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139087317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactate coordinated with exercise promoted the browning of inguinal white adipose tissue. 乳酸盐与运动的协调促进了腹股沟白色脂肪组织的棕色化。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-01-04 DOI: 10.1007/s13105-023-01004-9

Xuefei Chen, Yanjun Li, Jingbo Zhang, Wenhua Huang, Jie Su, Jing Zhang

{"title":"Lactate coordinated with exercise promoted the browning of inguinal white adipose tissue.","authors":"Xuefei Chen, Yanjun Li, Jingbo Zhang, Wenhua Huang, Jie Su, Jing Zhang","doi":"10.1007/s13105-023-01004-9","DOIUrl":"10.1007/s13105-023-01004-9","url":null,"abstract":"Lactate, an important exercise metabolite, induces white adipose tissue browning by upregulated uncoupling protein 1 (UCP1) expression. However, the function of lactate during browning of inguinal white adipose tissue (iWAT) caused by exercise is unclear. Here, we considered lactate as an exercise supplement and investigated the effects of chronic pre-exercise lactate administration on energy metabolism and adipose tissue browning. C57B/L6 male mice (5 weeks of age) were divided into six groups. We evaluated the changes in blood lactate levels in each group of mice after the intervention. Energy expenditure was measured after the intervention immediately by indirect calorimetry. The marker protein levels and gene expressions were determined by western-blot and quantitative real-time PCR. HIIT significantly decreased adipose tissue weight while increased energy expenditure and the expression of UCP1 in iWAT; however, these regulations were inhibited in the DCA+HIIT group. Compared with the MICT and LAC groups, long-term lactate injection before MICT led to lower WAT weight to body weight ratios and higher energy expenditure in mice. Furthermore, the marker genes of browning in iWAT, such as Ucp1 and Pparγ, were significantly increased in the LAC+MICT group than in the other groups, and the expression of monocarboxylate transporter-1 (Mct1) mRNA was also significantly increased. Lactate was involved in exercise-mediated browning of iWAT, and its mechanism might be the increased of lactate transport through MCT1 or PPARγ upregulation induced by exercise. These findings suggest exogenous lactate may be a new exercise supplement to regulate metabolism.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"303-315"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139087319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NCS-1 protein regulates TRPA1 channel through the PI3K pathway in breast cancer and neuronal cells. NCS-1 蛋白通过 PI3K 途径调节乳腺癌和神经细胞中的 TRPA1 通道。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-04-02 DOI: 10.1007/s13105-024-01016-z

Julio C Sánchez, Alexander Alemán, Juan F Henao, Juan C Olaya, Barbara E Ehrlich

{"title":"NCS-1 protein regulates TRPA1 channel through the PI3K pathway in breast cancer and neuronal cells.","authors":"Julio C Sánchez, Alexander Alemán, Juan F Henao, Juan C Olaya, Barbara E Ehrlich","doi":"10.1007/s13105-024-01016-z","DOIUrl":"10.1007/s13105-024-01016-z","url":null,"abstract":"The physical and functional interaction between transient receptor potential channel ankyrin 1 (TRPA1) and neuronal calcium sensor 1 (NCS-1) was assessed. NCS-1 is a calcium (Ca2+) sensor found in many tissues, primarily neurons, and TRPA1 is a Ca2+ channel involved not only in thermal and pain sensation but also in conditions such as cancer and chemotherapy-induced peripheral neuropathy, in which NCS-1 is also a regulatory component.We explored the interactions between these two proteins by employing western blot, qRT-PCR, co-immunoprecipitation, Ca2+ transient monitoring with Fura-2 spectrophotometry, and electrophysiology assays in breast cancer cells (MDA-MB-231) with different levels of NCS-1 expression and neuroblastoma cells (SH-SY5Y).Our findings showed that the expression of TRPA1 was directly correlated with NCS-1 levels at both the protein and mRNA levels. Additionally, we found a physical and functional association between these two proteins. Physically, the NCS-1 and TRPA1 co-immunoprecipitate. Functionally, NCS-1 enhanced TRPA1-dependent Ca2+ influx, current density, open probability, and conductance, where the functional effects depended on PI3K. Conclusion: NCS-1 appears to act not only as a Ca2+ sensor but also modulates TRPA1 protein expression and channel function in a direct fashion through the PI3K pathway. These results contribute to understanding how Ca2+ homeostasis is regulated and provides a mechanism underlying conditions where Ca2+ dynamics are compromised, including breast cancer. With a cellular pathway identified, targeted treatments can be developed for breast cancer and neuropathy, among other related diseases.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"451-463"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated clinical and prognostic analyses of mTOR/Hippo pathway core genes in hepatocellular carcinoma. 肝细胞癌中 mTOR/Hippo 通路核心基因的临床和预后综合分析。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-03-12 DOI: 10.1007/s13105-024-01015-0

Tianhang Feng, Ping Chen, Tao Wang, Chunyou Lai, Yutong Yao

{"title":"Integrated clinical and prognostic analyses of mTOR/Hippo pathway core genes in hepatocellular carcinoma.","authors":"Tianhang Feng, Ping Chen, Tao Wang, Chunyou Lai, Yutong Yao","doi":"10.1007/s13105-024-01015-0","DOIUrl":"10.1007/s13105-024-01015-0","url":null,"abstract":"Hepatocellular carcinoma (HCC) is one of the most aggressive and dismal cancers globally. Emerging evidence has established that mTOR and Hippo pathways are oncogenic drivers of HCC. However, the prognostic value of these pathways in HCC remains unclear. In this study, we aimed to develop a gene signature utilizing the mTOR/Hippo genes for HCC prognostication. A multiple stage strategy was employed to screen, and a 12-gene signature based on mTOR/Hippo pathways was constructed to predict the prognosis of HCC patients. The risk scores calculated by the signature were inversely correlated with patient prognosis. Validation of the signature in independent cohort confirmed its predictive power. Further analysis revealed molecular differences between high and low-risk groups at genomic, transcriptomic, and protein-interactive levels. Moreover, immune infiltration analysis revealed an immunosuppressive state in the high-risk group. Finally, the gene signature could predict the sensitivity to current chemotherapeutic drugs. This study demonstrated that combinatorial mTOR/Hippo gene signature was a robust and independent prognostic tool for survival prediction of HCC. Our findings not only provide novel insights for the molecular understandings of mTOR/Hippo pathways in HCC, but also have important clinical implications for guiding therapeutic strategies.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"439-449"},"PeriodicalIF":3.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of microRNA-363 during tumor progression and invasion microRNA-363 在肿瘤进展和侵袭过程中的作用

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 DOI: 10.1007/s13105-024-01022-1

Arya Nasimi Shad, Iman Akhlaghipour, Hawraa Ibrahim Alshakarchi, Ehsan Saburi, Meysam Moghbeli

{"title":"Role of microRNA-363 during tumor progression and invasion","authors":"Arya Nasimi Shad, Iman Akhlaghipour, Hawraa Ibrahim Alshakarchi, Ehsan Saburi, Meysam Moghbeli","doi":"10.1007/s13105-024-01022-1","DOIUrl":"https://doi.org/10.1007/s13105-024-01022-1","url":null,"abstract":"Recent progresses in diagnostic and therapeutic methods have significantly improved prognosis in cancer patients. However, cancer is still considered as one of the main causes of human deaths in the world. Late diagnosis in advanced tumor stages can reduce the effectiveness of treatment methods and increase mortality rate of cancer patients. Therefore, investigating the molecular mechanisms of tumor progression can help to introduce the early diagnostic markers in these patients. MicroRNA (miRNAs) has an important role in regulation of pathophysiological cellular processes. Due to their high stability in body fluids, they are always used as the non-invasive markers in cancer patients. Since, miR-363 deregulation has been reported in a wide range of cancers, we discussed the role of miR-363 during tumor progression and metastasis. It has been reported that miR-363 has mainly a tumor suppressor function through the regulation of transcription factors, apoptosis, cell cycle, and structural proteins. MiR-363 also affected the tumor progression via regulation of various signaling pathways such as WNT, MAPK, TGF-β, NOTCH, and PI3K/AKT. Therefore, miR-363 can be introduced as a probable therapeutic target as well as a non-invasive diagnostic marker in cancer patients.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"3 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1. 弹性蛋白衍生肽诱导的序列酸裂解会损害 3T3-L1 脂肪细胞中胰岛素与其受体之间的相互作用。

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-05-01 Epub Date: 2024-02-23 DOI: 10.1007/s13105-024-01010-5

Alexandre Guillot, Kevin Toussaint, Lucrece Ebersold, Hassan ElBtaouri, Emilie Thiebault, Tarik Issad, Franck Peiretti, Pascal Maurice, Hervé Sartelet, Amar Bennasroune, Laurent Martiny, Manuel Dauchez, Laurent Duca, Vincent Durlach, Béatrice Romier, Stéphanie Baud, Sébastien Blaise

{"title":"Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1.","authors":"Alexandre Guillot, Kevin Toussaint, Lucrece Ebersold, Hassan ElBtaouri, Emilie Thiebault, Tarik Issad, Franck Peiretti, Pascal Maurice, Hervé Sartelet, Amar Bennasroune, Laurent Martiny, Manuel Dauchez, Laurent Duca, Vincent Durlach, Béatrice Romier, Stéphanie Baud, Sébastien Blaise","doi":"10.1007/s13105-024-01010-5","DOIUrl":"10.1007/s13105-024-01010-5","url":null,"abstract":"The insulin receptor (IR) plays an important role in insulin signal transduction, the defect of which is believed to be the root cause of type 2 diabetes. In 3T3-L1 adipocytes as in other cell types, the mature IR is a heterotetrameric cell surface glycoprotein composed of two α subunits and two β subunits. Our objective in our study, is to understand how the desialylation of N-glycan chains, induced by elastin-derived peptides, plays a major role in the function of the IR. Using the 3T3-L1 adipocyte line, we show that removal of the sialic acid from N-glycan chains (N893 and N908), induced by the elastin receptor complex (ERC) and elastin derived-peptides (EDPs), leads to a decrease in the autophosphorylation activity of the insulin receptor. We demonstrate by molecular dynamics approaches that the absence of sialic acids on one of these two sites is sufficient to generate local and general modifications of the structure of the IR. Biochemical approaches highlight a decrease in the interaction between insulin and its receptor when ERC sialidase activity is induced by EDPs. Therefore, desialylation by EDPs is synonymous with a decrease of IR sensitivity in adipocytes and could thus be a potential source of insulin resistance associated with diabetic conditions.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":"363-379"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139931606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in extracellular vesicle homeostasis as it relates to cardiovascular diseases 细胞外囊泡稳态与心血管疾病的关系研究进展

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-30 DOI: 10.1007/s13105-024-01027-w

Yunan Kang, Wenqian Wu, Yi Yang, Jinxi Luo, Yajie Lu, Luchang Yin, Xiaodong Cui

{"title":"Progress in extracellular vesicle homeostasis as it relates to cardiovascular diseases","authors":"Yunan Kang, Wenqian Wu, Yi Yang, Jinxi Luo, Yajie Lu, Luchang Yin, Xiaodong Cui","doi":"10.1007/s13105-024-01027-w","DOIUrl":"https://doi.org/10.1007/s13105-024-01027-w","url":null,"abstract":"Extracellular vesicles (EVs) are involved in both physiological and pathological processes in many organ systems and are essential in mediating intercellular communication and maintaining organismal homeostasis. It is helpful to propose new strategies for disease treatment by elucidating the mechanisms of EV release and sorting. An increasing number of studies have shown that there is specific homeostasis in EVs, which is helpful for the human body to carry out physiological activities. In contrast, an EV homeostasis im-balance promotes or accelerates disease onset and development. Alternatively, regulating the quality of EVs can maintain homeostasis and even achieve the purpose of treating conditions. An analysis of the role of EV homeostasis in the onset and development of cardiovascular disease is presented in this review. This article also summarizes the methods that regulate EV homeostasis and their application in cardiovascular diseases. In particular, this study focuses on the connection between EV steady states and the cardiovascular system and the potential value of EVs in treating cardiovascular diseases.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"23 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovalbumin promotes innate immune response of Caenorhabditis elegans through DAF-16 and SKN-1 pathways in insulin/IGF-1 signaling 卵清蛋白通过胰岛素/IGF-1 信号转导中的 DAF-16 和 SKN-1 通路促进秀丽隐杆线虫的先天性免疫反应

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-18 DOI: 10.1007/s13105-024-01021-2

Haibing Pei, Zhiyin Lin, Kexin Yao, Yeqing Luo, Ping Tong, Hongbing Chen, Yong Wu, Zhihua Wu, Jinyan Gao

{"title":"Ovalbumin promotes innate immune response of Caenorhabditis elegans through DAF-16 and SKN-1 pathways in insulin/IGF-1 signaling","authors":"Haibing Pei, Zhiyin Lin, Kexin Yao, Yeqing Luo, Ping Tong, Hongbing Chen, Yong Wu, Zhihua Wu, Jinyan Gao","doi":"10.1007/s13105-024-01021-2","DOIUrl":"https://doi.org/10.1007/s13105-024-01021-2","url":null,"abstract":"Ovalbumin (OVA) is a major allergen in eggs and could induce severe allergic reactions in sensitive individuals, where the innate immune system works as a regulator. The mechanism of how innate immunity adjusts to food allergy is relatively well-studied, however, the effects of allergen uptake on the innate immune system remain unclear. Therefore, the Caenorhabditis elegans (C. elegans) model was utilized to assess the effects of OVA on its innate immune system. OVA enhanced the immune response of C. elegans with higher survival rates under Pseudomonas aeruginosa infection. Moreover, sustaining OVA treatment improved the health states that were reflected in the prolonged lifespan, alleviated oxidative stress, accelerated growth, and promoted motility. RNA-sequencing analysis and the slow-killing assays in the mutants of insulin/IGF-1 signaling (IIS)-related genes confirmed that IIS was necessary for OVA to regulate innate immunity. Besides, OVA activated SKN-1 temporarily and facilitated the nuclear localization of DAF-16 for improving immunity and health status in C. elegans. Together, OVA could enhance the innate immune responses via DAF-16 and SKN-1 pathways in the IIS of C. elegans, and this work will provide novel insights into the regulation of innate immunity by OVA in higher organisms.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":"12 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fasting and fasting-mimicking conditions in the cancer immunotherapy era 癌症免疫疗法时代的禁食和模拟禁食条件

IF 3.4 3区生物学

Journal of physiology and biochemistry Pub Date : 2024-04-08 DOI: 10.1007/s13105-024-01020-3

Ruben Pio, Yaiza Senent, Beatriz Tavira, Daniel Ajona

引用次数: 0