Journal of physiology and biochemistry最新文献

Anti-steatotic effect of Opuntia ficus-indica extracts rich in betalains and phenolics from fruit peel and pulp of different varieties in in vitro models. 不同品种无花果果皮和果肉中富含甜菜素和酚类物质提取物的体外抗脂肪变性作用。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-20 DOI: 10.1007/s13105-025-01097-4

Irene Besné-Eseverri, Jenifer Trepiana, Itziar Eseberri, Andrea Gómez-Maqueo, M Pilar Cano, Joao Tomé-Carneiro, Alberto Dávalos, María P Portillo

{"title":"Anti-steatotic effect of Opuntia ficus-indica extracts rich in betalains and phenolics from fruit peel and pulp of different varieties in in vitro models.","authors":"Irene Besné-Eseverri, Jenifer Trepiana, Itziar Eseberri, Andrea Gómez-Maqueo, M Pilar Cano, Joao Tomé-Carneiro, Alberto Dávalos, María P Portillo","doi":"10.1007/s13105-025-01097-4","DOIUrl":"10.1007/s13105-025-01097-4","url":null,"abstract":"Opuntia ficus-indica exhibits antioxidant, anti-inflammatory and anti-hyperglycemic properties, making it a promising candidate for the prevention and treatment of metabolic dysfunction-associated fatty liver disease. However, its effects on triglyceride accumulation remain largely unexplored. The aim of the present study is to evaluate the anti-steatotic effect of peel and pulp extracts from different varieties of Opuntia ficus-indica fruits (Pelota, Colorada and Sanguinos) in hepatic murine in vitro models, using both AML12 hepatocytes and hepatic organoids. The pulp extracts of Pelota and Colorada varieties, as well as both peel and pulp extracts of Sanguinos, were effective in reducing palmitic acid-induced triglyceride accumulation in AML12 hepatocytes. The doses that caused the greatest triglyceride reduction were 50 µg/mL of the pulp of Pelota and 100 µg/mL for the other extracts. The potential mechanisms underlying these effects seem to be associated, at least in part, with the inhibition of fatty acid uptake and triglyceride assembly. The pulp extract of the Colorada variety was able to prevent triglyceride accumulation also in hepatic organoids, likely due to downregulation of fatty acid transporters. These findings underscore the value of employing diverse in vitro models (e.g., 2D, 3D) to investigate the potential effects of these extracts, and suggest that the pulp extract of the Colorada variety may be effective in preventing steatosis.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Acute pancreatitis experimental models, advantages and disadvantages. 更正：急性胰腺炎实验模型，优缺点。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-18 DOI: 10.1007/s13105-025-01102-w

Genaro J Rosales-Muñoz, Verónica Souza-Arroyo, Leticia Bucio-Ortiz, Roxana U Miranda-Labra, Luis E Gomez-Quiroz, María Concepción Gutiérrez-Ruiz

引用次数: 0

Expression landscape of epigenetic genes in human hepatocellular carcinoma. 人肝细胞癌中表观遗传基因的表达图谱。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-12 DOI: 10.1007/s13105-025-01095-6

Borja Castelló-Uribe, Amaya López-Pascual, Jasmin Elurbide, Elena Adán-Villaescusa, Emiliana Valbuena-Goiricelaya, Luz A Martinez-Perez, Iker Uriarte, M Ujúe Latasa, Bruno Sangro, María Arechederra, Carmen Berasain, Matías A Avila, Maite G Fernández-Barrena

{"title":"Expression landscape of epigenetic genes in human hepatocellular carcinoma.","authors":"Borja Castelló-Uribe, Amaya López-Pascual, Jasmin Elurbide, Elena Adán-Villaescusa, Emiliana Valbuena-Goiricelaya, Luz A Martinez-Perez, Iker Uriarte, M Ujúe Latasa, Bruno Sangro, María Arechederra, Carmen Berasain, Matías A Avila, Maite G Fernández-Barrena","doi":"10.1007/s13105-025-01095-6","DOIUrl":"https://doi.org/10.1007/s13105-025-01095-6","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the most common primary liver tumor, often arising in the context of chronic liver disease. Despite recent advances in systemic therapies, including the use of immune checkpoint inhibitors (ICIs), clinical outcomes remain suboptimal, with many patients exhibiting primary or acquired resistance. Accumulating evidence indicates that the dysregulation of epigenetic mechanisms contributes to HCC development, and may also play a crucial role in shaping the tumor immune microenvironment, influencing responses to treatments. In this study, we analyzed the expression profiles of a comprehensive set of epigenetic regulators across publicly available transcriptomic datasets of HCC and non-tumoral liver tissues. Our findings reveal a consistent dysregulation of key epigenetic modifiers, particularly those involved in DNA methylation and histone modification. Furthermore, our analysis underscores the need for a deeper understanding of the epigenetic landscape of HCC, as specific epigenetic patterns are directly associated with disease development, the major mutational, immune, and transcriptional subclasses of HCC, and patient clinical outcomes. Our study provides a foundation for integrating epigenetic biomarkers into patient stratification and therapeutic decision-making. A more comprehensive analysis of epigenetic alterations could pave the way for novel predictive markers and combination strategies that could enhance the efficacy of ICIs in HCC.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of dietary camelina oil intake on skeletal muscle metabolism and sarcopenia in older mice. 膳食中摄入亚麻荠油对老年小鼠骨骼肌代谢和肌肉减少症的影响。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-11 DOI: 10.1007/s13105-025-01089-4

Jean-Paul Rigaudière, Christophe Montaurier, Philippe Denis, Olivier Le Bacquer, Mathieu Rambeau, Chrystèle Jouve, Julia Soullier, Justine Bertrand-Michel, David M Mutch, Frederic Capel

{"title":"Effects of dietary camelina oil intake on skeletal muscle metabolism and sarcopenia in older mice.","authors":"Jean-Paul Rigaudière, Christophe Montaurier, Philippe Denis, Olivier Le Bacquer, Mathieu Rambeau, Chrystèle Jouve, Julia Soullier, Justine Bertrand-Michel, David M Mutch, Frederic Capel","doi":"10.1007/s13105-025-01089-4","DOIUrl":"https://doi.org/10.1007/s13105-025-01089-4","url":null,"abstract":"The age-related decrease in skeletal muscle mass and function, mostly known as sarcopenia, increases the risk of mobility impairments, chronic disease and early mortality. Physical activity and targeted dietary approaches are the most effective intervention to prevent or limit sarcopenia. Omega-3 polyunsaturated fatty acids (PUFA) could alleviate some aspects of age-related diseases. We investigated the effect of a chronic intake of a diet containing a high content of omega-3 PUFA in old mice exposed to a normocaloric or an obesogenic diet. Female C57BL/6J mice received a low-fat or a high-fat diet containing 5 or 6%, respectively, of camelina oil (comprising 27% omega-3 PUFA) for 18 weeks and were compared to animals receiving the similar diets containing high-oleic sunflower oil instead. Circulating parameters, calorimetry and physical performances were evaluated as well as muscle lipid content and molecular adaptations. Consumption of camelina oil increased omega-3 PUFA content in biological membranes, as well as circulating levels of anti-inflammatory oxylipin mediators. High-fat diets induced changes in body composition but these effects were not affected by the intake of camelina oil. However, camelina oil consumption increased motor coordination in the low-fat condition. Some lipidomic adaptations were observed in relation to oil intake. Variations in plasma levels of glycerol, free fatty acids and muscle gene expression suggested improved lipid homeostasis in groups receiving camelina oil. In conclusion, the consumption of an energy-balanced diet with a high content of omega-3 PUFA provided by camelina oil could provide benefits on muscle health. CLINICAL TRIAL REGISTRATION: Not applicable.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-320-3p regulates apelin and TGF-β/SMAD3 signaling in hypobaric hypoxia exposed rats to induce skeletal muscle atrophy. miR-320-3p调节低氧暴露大鼠apelin和TGF-β/SMAD3信号，诱导骨骼肌萎缩。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-11 DOI: 10.1007/s13105-025-01100-y

Samrita Mondal, Sukanya Srivastava, Swati Srivastava, Richa Rathor, Geetha Suryakumar

{"title":"miR-320-3p regulates apelin and TGF-β/SMAD3 signaling in hypobaric hypoxia exposed rats to induce skeletal muscle atrophy.","authors":"Samrita Mondal, Sukanya Srivastava, Swati Srivastava, Richa Rathor, Geetha Suryakumar","doi":"10.1007/s13105-025-01100-y","DOIUrl":"https://doi.org/10.1007/s13105-025-01100-y","url":null,"abstract":"Emerging research on microRNA has decoded its crucial role in gene regulation, development and diseases. Skeletal muscle atrophy is reported in several chronic diseases as well as prolonged stay at high altitude. miR-320-3p is reported to be upregulated in various chronic diseases including cancer, heart diseases, diabetes, and chronic kidney diseases. The present study evaluates the role of miR-320-3p expression in regulating apelin and its downstream signaling under hypobaric hypoxia (HH) at high altitude. The expression of miR-320-3p was found to be upregulated during 7days HH (7DHH) exposure at 25,000 ft as compared to control group. The targets for miR-320-3p were retrieved from miRWalk 3.0, TargetScan 8.0, miRTarBase 10.0 databases in Rattus norvegicus. Using in silico approach, 26 myokines were screened out of total 14,435 targets of rno-miR-320-3p and levels of few myokines were experimentally validated. The expression of apelin, decorin, osteocrin, meteorin-like myokines were found to be significantly decreased while myostatin was significantly increased during HH exposure as compared to control rats. Enhanced expression of Tgfb and p-Smad3 under 7DHH indicated activation of protein degradation pathways. Expression of Pgc1a and Nrf2, the critical regulators of mitochondrial biogenesis, were significantly decreased under HH. Thus, increased expression of miR-320-3p regulate apelin and modulate downstream signaling via attenuation of mitochondrial biogenesis and myogenesis. Hence, miR-320-3p and myokines play pivotal role to regulate skeletal muscle atrophy. Further research on potential targets of miR-320-3p regulating the muscle mass may lead to the development of novel therapeutics in personalized medicine to combat skeletal muscle diseases.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex impact on daily activity and physiological, metabolic and hormonal responses to different photoperiod regimens in diet-induced obese fischer 344 rats. 性别对饮食诱导肥胖fischer 344大鼠日常活动及不同光周期方案下生理、代谢和激素反应的影响

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-10 DOI: 10.1007/s13105-025-01075-w

Saioa Gómez-Roncal, Aina Gironès-Garreta, Manuel Suárez, Begoña Muguerza, Gerard Aragonès, Miquel Mulero, Anna Arola-Arnal

引用次数: 0

Molecular biomarkers for the prognosis of breast cancer: role of amino acid metabolism genes. 乳腺癌预后的分子生物标志物：氨基酸代谢基因的作用。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-10 DOI: 10.1007/s13105-025-01088-5

Yudong Zhou, Shibo Yu, Lizhe Zhu, Yalong Wang, Chenglong Duan, Danni Li, Jinsui Du, Jiaqi Zhang, Jianing Zhang, Ruichao Ma, Jianjun He, Yu Ren, Bin Wang

{"title":"Molecular biomarkers for the prognosis of breast cancer: role of amino acid metabolism genes.","authors":"Yudong Zhou, Shibo Yu, Lizhe Zhu, Yalong Wang, Chenglong Duan, Danni Li, Jinsui Du, Jiaqi Zhang, Jianing Zhang, Ruichao Ma, Jianjun He, Yu Ren, Bin Wang","doi":"10.1007/s13105-025-01088-5","DOIUrl":"https://doi.org/10.1007/s13105-025-01088-5","url":null,"abstract":"The development of precise molecular biomarkers for breast cancer prognosis holds immense potential to improve treatment outcomes. This study aimed to investigate the role of amino acid metabolism genes as predictive markers for breast cancer prognosis and their association with the immune-tumour microenvironment. By employing advanced machine learning algorithms and bioinformatics analysis techniques, the impact of amino acid metabolism-related genes (AAMRGs) on the immune status and overall survival of patients with breast cancer was examined. An AAMRG-based risk model was established to assess the prognostic significance. Validated risk models (AIMP2, IYD, and QARS1) accurately predicted patient outcomes [1 y: 0.87 (0.96-0.78); 3 y: 0.82 (0.87-0.76); 5 y: 0.80 (0.86-0.75)]. Furthermore, this study revealed evidence suggesting that QARS1 may influence breast cancer cell proliferation through methionine metabolism. This analysis provides valuable insights into the mechanisms of breast cancer, emphasizing the significance of AAMRGs as prognostic biomarkers and potential therapeutic targets for optimizing personalized treatment strategies.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between MST1-Hippo and Wnt/β-Catenin, Notch, and PI3K/Akt pathways in cardiac physiology and pathology. MST1-Hippo与Wnt/β-Catenin、Notch和PI3K/Akt通路在心脏生理和病理中的串扰

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-09 DOI: 10.1007/s13105-025-01099-2

Jin Wang, Kai Jing, Vadim Mitrokhin, Stanislav Schileyko, Anastasija Rodina, Alexandra Zolotareva, Valentin Zolotarev, Natalia Bocharnikova, Dmitry Kaminer, Emilija Antova, Radoslav Stojchevski, Nikola Hadzi-Petrushev, Dimiter Avtanski, Andre Kamkin, Mitko Mladenov

引用次数: 0

PLAC8 as a potential therapeutic target for myocardial infarction: unraveling the molecular mechanisms. PLAC8作为心肌梗死的潜在治疗靶点：揭示其分子机制。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-06-05 DOI: 10.1007/s13105-025-01098-3

Yifan Tong, Xin Huang, Wei Qian, Lijuan Liu

{"title":"PLAC8 as a potential therapeutic target for myocardial infarction: unraveling the molecular mechanisms.","authors":"Yifan Tong, Xin Huang, Wei Qian, Lijuan Liu","doi":"10.1007/s13105-025-01098-3","DOIUrl":"https://doi.org/10.1007/s13105-025-01098-3","url":null,"abstract":"The incidence of myocardial infarction (MI) has been increasing in recent years, and the cause of acute myocardial infarction is apoptosis due to insufficient coronary myocardial blood supply. PLAC8 is a critical gene in the disease process of MI through GEO database research and analysis of differentially expressed genes (DEGs). In this study, in mice with myocardial infarction caused by surgical ligation of the left anterior descending coronary artery (LAD) and hypoxia-induced H9C2 cells as a model, the myocardium of the model group was found to show severe cardiomyocyte disorders, apoptosis of inflammatory cell infiltration, and ischemic state by HE, TTC, and Tunel staining. The expression of PLAC8 was reduced in the disease model by PCR and Western blot, and the expression of cle-Casp3 and Bax was also found to be high. However, overexpression of PLAC8 in the disease model reversed these processes. MEK/ERK and P65 are the core signaling pathways in the MI model. In this study, we found that the therapeutic effect of PLAC8 was related to the inhibition of the MEK/ERK signaling pathway by overexpression of PLAC8 and antagonism of the MEK/ERK signaling pathway. In conclusion, the inhibition of the MEK/ERK signaling pathway by PLAC8 under hypoxic conditions reduces apoptosis in H9c2 cells, which may provide new ideas for the determination and treatment of MI.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The importance of vitamin D levels in patients with inflammatory bowel disease. 维生素D水平对炎症性肠病患者的重要性。

IF 3.7 3区生物学

Journal of physiology and biochemistry Pub Date : 2025-05-26 DOI: 10.1007/s13105-025-01096-5

Evgenia Koureta, Pantelis Karatzas, Panagiotis Kanellopoulos, Angeliki Papapanagiotou, Vasileios Lekakis, Giorgos Bamias, Andreas Koutsoumpas, George Karamanolis, Jiannis Vlachogiannakos, Athanasios G Papavassiliou, George V Papatheodoridis

{"title":"The importance of vitamin D levels in patients with inflammatory bowel disease.","authors":"Evgenia Koureta, Pantelis Karatzas, Panagiotis Kanellopoulos, Angeliki Papapanagiotou, Vasileios Lekakis, Giorgos Bamias, Andreas Koutsoumpas, George Karamanolis, Jiannis Vlachogiannakos, Athanasios G Papavassiliou, George V Papatheodoridis","doi":"10.1007/s13105-025-01096-5","DOIUrl":"https://doi.org/10.1007/s13105-025-01096-5","url":null,"abstract":"The possible role of vitamin D (VD) in the pathogenesis of inflammatory bowel disease (IBD) and the associations between VD levels and IBD activity remain unclarified. We aimed to assess VD levels in IBD patients and their associations with IBD activity. We evaluated VD levels in Greek patients aged 18-75 years old with Crohn's disease (CD) or ulcerative colitis (UC). Patients were ineligible under the following conditions: history of enterectomy/right colectomy, receiving VD or agent(s) interfering with VD metabolism during the last three months and any comorbidities that influence VD levels. Epidemiologic characteristics, clinical course, laboratory investigations, endoscopic and histologic findings were recorded. In total, 122 patients with CD and 71 with UC were included. Most of them had low levels of VD (90% of CD and 91.5% of UC patients). Patients with clinically active CD or UC had lower levels of VD compared to those in remission (p = 0.009 and p = 0.033, respectively).CD patients with low levels of VD had higher CRP and stool calprotectin compared to those with normal levels of VD (P = 0.032 and P = 0.002, respectively). In UC, patients with pancolitis had lower VD levels compared to patients with proctitis (P = 0.036). In conclusion, the majority of Greek IBD patients have low levels of VD. Clinical activity is related to lower levels of VD. Low compared to normal levels of VD in CD patients are associated with higher CRP and calprotectin levels, so VD levels might serve as an activity marker.","PeriodicalId":16779,"journal":{"name":"Journal of physiology and biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0