{"title":"Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana","authors":"Moeka Fujita, Tomoya Tanaka, Miyuki Kusajima, Kengo Inoshima, Futo Narita, Hidemitsu Nakamura, Tadao Asami, Akiko Maruyama-Nakashita, Hideo Nakashita","doi":"10.1584/jpestics.d24-019","DOIUrl":"https://doi.org/10.1584/jpestics.d24-019","url":null,"abstract":"</p><p>Strigolactones (SLs) are a class of plant hormones that play several roles in plants, such as suppressing shoot branching and promoting arbuscular mycorrhizal symbiosis. The positive regulation of plant disease resistance by SLs has recently been demonstrated by analyses using SL-related mutants. In Arabidopsis, SL-mediated signaling has been reported to modulate salicylic acid-mediated disease resistance, in which the priming of plant immunity plays an important role. In this study, we analyzed the effect of the synthetic SL analogue <i>rac</i>-GR24 on resistance against necrotrophic pathogen <i>Botrytis cinerea</i>. In <i>rac</i>-GR24-treated plants, disease resistance against <i>B. cinerea</i> was enhanced in an ethylene- and camalexin-dependent manners. Expression of the ethylene-related genes and the camalexin biosynthetic gene and camalexin accumulation after pathogen infection were enhanced by immune priming in <i>rac</i>-GR24-treated plants. These suggest that SL-mediated immune priming is effective for many types of resistance mechanisms in plant self-defense systems.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-019/figure/advpub_D24-019.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"107 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry","authors":"Takamitsu Otake, Keisuke Nakamura, Naoyuki Hirabayashi, Takaho Watanabe","doi":"10.1584/jpestics.d24-021","DOIUrl":"https://doi.org/10.1584/jpestics.d24-021","url":null,"abstract":"</p><p>The objective of the present study is to provide reliable concentration values as assigned values for target pesticides in brown rice samples used in proficiency testing (PT) organized by the Hatano Research Institute (HRI). The test samples for PT were prepared by immersing brown rice in the pesticide solution and using a spray dryer by the HRI. Homogeneity and stability assessments were performed for PT samples, and the relative uncertainties due to inhomogeneity and instability were 0.58 %–0.78 % and 0 %–0.96 %, respectively. Quantification for the assigned values of target pesticides by the National Metrology Institute of Japan (NMIJ) was carried out using the multiple analytical methods including Japanese official analytical method, QuEChERS, and modified QuEChERS, which were combined with isotope dilution mass spectrometry, to ensure the reliability of the analytical results. The NMIJ assigned values were 0.065±0.004 mg/kg for chlorpyrifos, 0.217±0.012 mg/kg for diazinon, 0.138±0.008 mg/kg for fenitrothion, and 0.138±0.008 mg/kg for malathion.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-021/figure/advpub_D24-021.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"95 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS","authors":"Hiroto Tamura","doi":"10.1584/jpestics.d24-006","DOIUrl":"https://doi.org/10.1584/jpestics.d24-006","url":null,"abstract":"</p><p>As Sustainable Development Goals (SDGs) and the realities of climate change become widely accepted around the world, the next-generation of integrated pest management will become even more important for establishing a sustainable food production system. To meet the current challenge of food security and climate change, biological control has been developed as one sustainable crop protection technology. However, most registered bacteria are ubiquitous soil-borne bacteria that are closely related to food poisoning and spoilage bacteria. Therefore, this review outlined (1) the mechanism of action of bacterial pesticides, (2) potential concerns about secondary contamination sources associated with past food contamination, and, as a prospective solution, focused on (3) principles and methods of bacterial identification, and (4) the possibility of identifying residual bacteria based on mass spectrometry.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-006/figure/advpub_D24-006.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"10 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141739968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-throughput screening for novel Bacillus thuringiensis insecticidal proteins revealed evidence that the bacterium exchanges Domain III to enhance its insecticidal activity","authors":"Ruth Cong, Jing-Tong Hou, Takashi Yamamoto","doi":"10.1584/jpestics.d24-011","DOIUrl":"https://doi.org/10.1584/jpestics.d24-011","url":null,"abstract":"</p><p>Approximately 3000 <i>Bacillus thuringiensis</i> (Bt) isolates were screened to discover novel three-domain (3D) Cry proteins active against <i>Helicoverpa zea</i> (corn earworm). From 400 active isolates found during the primary screening, Cry1Ac and Cry2A, which are known to be active against <i>H. zea</i>, were removed using multiplex-primer PCR and high-throughput column chromatography. This process reduced the number of active cultures to 48. DNA segments encoding Domain III of these 48 cultures were amplified by PCR and sequenced. Sequencing revealed two novel Cry1B-type Domain IIIs. Further sequencing of the flanking regions of these domains revealed that one was part of Cry1Bj (GenBank: KT952325). However, the other Domain III lacked Domains I and II. Instead, this Domain III was associated with two open reading frames, ORF1 and ORF2. ORF1 was identified as an ATP-binding protein, and ORF2 as an ATPase, suggesting that Bt exchanges Domain III among homologous Cry proteins.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-011/figure/advpub_D24-011.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"5 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141584872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nanoencapsulated deltamethrin combined with indoxacarb: An effective synergistic association against aphids","authors":"Marine Galloux, Guillaume Bastiat, Corinne Lefrancois, Véronique Apaire-Marchais, Caroline Deshayes","doi":"10.1584/jpestics.d24-003","DOIUrl":"https://doi.org/10.1584/jpestics.d24-003","url":null,"abstract":"</p><p>Widespread pesticide use for decades has caused environmental damage, biodiversity loss, serious human and animal health problems, and resistance to insecticides. Innovative strategies are needed to reduce treatment doses in pest management and to overcome insecticide resistance. In the present study, combinations of indoxacarb, an oxadiazine insecticide, with sublethal concentrations of deltamethrin encapsulated in lipid nanocapsules, have been tested on the crop pest <i>Acyrthosiphon pisum</i>. <i>In vivo</i> toxicological tests on <i>A. pisum</i> larvae have shown a synergistic effect of nanoencapsulated deltamethrin with a low dose of indoxacarb. Furthermore, the stability of deltamethrin nanoparticles has been demonstrated <i>in vitro</i> under different mimicking environmental conditions. In parallel, the integrity and stability of lipid nanoparticles in the digestive system of aphid larvae over time have been observed by Förster Resonance Energy Transfer (FRET) imaging. Thus, the deltamethrin nanocapsules/indoxacarb synergistic association is promising for the development of future formulations against pest insects to reduce insecticide doses.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-003/figure/advpub_D24-003.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"59 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141575818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advantageous properties of a new fungicide, isofetamid","authors":"Shuko Nishimi, Yuzuka Abe, Nanami Kuwahara, Akihiro Nishimura, Shintaro Tsukuda, Satoshi Araki, Kosuke Tsunematsu, Yohei Fukumori, Munekazu Ogawa, Kazumi Suzuki, Shigeru Mitani","doi":"10.1584/jpestics.d23-067","DOIUrl":"https://doi.org/10.1584/jpestics.d23-067","url":null,"abstract":"</p><p>The fungicidal properties of a new fungicide, isofetamid, were examined to assess its antifungal spectrum, mode of action, and effects on the infection process of <i>Botrytis cinerea</i>. Additionally, we investigated its fungicidal activity against isolates of <i>B. cinerea</i> resistant to existing fungicides. In mycelial growth inhibition tests, isofetamid exhibited excellent fungicidal activity against ascomycetes but showed no activity against basidiomycetes and oomycetes. Respiratory enzyme assay using mitochondria revealed that isofetamid inhibited succinate dehydrogenase activity prepared from <i>B. cinerea</i> and other ascomycetes fungi used in the study. On the other hand, the activity of mitochondria prepared from <i>Pythium</i>, potato and rat were not inhibited. Isofetamid inhibited also many stages of the infection processes in <i>B. cinerea</i>. Furthermore, it exhibited high fungicidal activity against <i>B. cinerea</i> isolates that were resistant to existing fungicides.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D23-067/figure/advpub_D23-067.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"19 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141196171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolomics analysis of the effects of chelerythrine on <i>Ustilaginoidea virens</i>.","authors":"Qinghui Wei, Xihai Zhai, Weifeng Song, Zhiyong Li, Yaqing Pan, Baoying Li, Zhanli Jiao, Zhenghao Shi, Jiangtao Yu","doi":"10.1584/jpestics.D23-065","DOIUrl":"10.1584/jpestics.D23-065","url":null,"abstract":"<p><p>Rice false smut (RFS) caused by <i>Ustilaginoide</i>a virens is widely distributed in major rice-producing regions. Previous studies have shown that treating RFS with chelerythrine can decrease the germination of fungus spores by 86.7% and induce fungal cell apoptosis. In the present study, the effects of chelerythrine on the metabolism of <i>U. virens</i> explored using metabolomics and analyses of differentially accumulated metabolites and altered metabolic pathways. The top 15 metabolites in random forest analysis were significantly different between groups. In positive ion mode, purine, phenylalanine metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, pyrimidine metabolism, and nitrogen metabolism were dominant. Alanine, aspartate, glutamate metabolism, and phenylalanine metabolism were enriched in negative ion mode. Differentially expressed genes and altered metabolic pathways of <i>U. virens</i> were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine and provide a theoretical basis for targeted drug delivery.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 2","pages":"104-113"},"PeriodicalIF":2.4,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci","authors":"Yui Tomizawa, Misato Aizawa, Akiya Jouraku, Shoji Sonoda","doi":"10.1584/jpestics.d24-009","DOIUrl":"https://doi.org/10.1584/jpestics.d24-009","url":null,"abstract":"</p><p>Using PCR-Restriction Fragment Length Polymorphism (RFLP) with mitochondrial cytochrome <i>c</i> oxidase subunit I sequences, we examined the reproductive modes of female adults of <i>Thrips tabaci</i> collected at 54 sites across Japan. Results showed the presence of heteroplasmic insects harboring mitochondria associated with arrhenotoky and thelytoky. Using the insects, we also applied PCR-RFLP to examine the genotypes for the amino acid mutation (T929I) site involved in pyrethroid resistance. Findings showed the presence of thelytokous heterozygotes under the circumstance that most arrhenotokous insects are resistant homozygotes, and many thelytokous insects are susceptible homozygotes. These results suggest that, in the field, genetic exchange occurs between insects through of both reproductive modes. A survey of the genotypes for the other amino acid mutations using nucleotide sequencing showed a decline of insects with an M918T and L1014F pair and an increase of insects with M918L. These results suggest the evolutional progression of amino acid mutations associated with pyrethroid resistance in <i>T. tabaci.</i></p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-009/figure/advpub_D24-009.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"53 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitative structure–activity relationship of 2,6-dimethoxy-N-(3-(4-substituted phenyl)isoxazol-5-yl)benzamide for the inhibition of chitin synthesis","authors":"Kotaro Mori, Masahiro Miyashita, Soichirou Mori, Norio Shibata, Yoshiaki Nakagawa","doi":"10.1584/jpestics.d24-004","DOIUrl":"https://doi.org/10.1584/jpestics.d24-004","url":null,"abstract":"</p><p>Previously, we found that 5-(2,6-dimethoxybenzoylamino)-3-phenylisoxazoles (IOXs) inhibit chitin synthesis in the cultured integument of <i>Chilo suppressalis</i>. In this study, IOXs with various substituents at the <i>para</i>-position of the 3-phenyl ring were synthesized, and the concentrations required to inhibit chitin synthesis to 50% (IC<sub>50</sub>) were determined for all compounds. The introduction of halogens—such as F, Cl, and Br—and small alkyls—such as Me, Et, Pr, and <i>n</i>-Bu—at the 3-phenyl ring slightly enhanced the activity. However, the activity decreased drastically with the introduction of NO<sub>2</sub>, CF<sub>3</sub>, and <i>t</i>-Bu. The quantitative analysis of the substituent effect at the 3-phenyl ring on chitin-synthesis inhibition using the Hansch-Fujita method showed that the hydrophobic substituent with the optimum value was favored for the activity, but the bulky substituent in terms of <i>E</i><sub><i>s</i></sub> was detrimental to the activity.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-004/figure/advpub_D24-004.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"33 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodegradation of nitenpyram (neonicotinoid insecticide) by endophytic bacterium, Bacillus thuringiensis strain NIT-2, isolated from neonicotinoid-treated plant samples","authors":"Md. Tareq Bin Salam, Koji Ito, Ryota Kataoka","doi":"10.1584/jpestics.d24-002","DOIUrl":"https://doi.org/10.1584/jpestics.d24-002","url":null,"abstract":"</p><p>Nitenpyram (neonicotinoid insecticide) is commonly used for crop protection from pests. Currently, due to its widespread use, the nitenpyram accumulation in the environment is anticipated to be high. Hence, the removal of nitenpyram residue from the environment is essential. However, the biodegradation of nitenpyram by endophytes is still unreported. Therefore, we aimed to isolate and identify a bacterial strain capable of degrading nitenpyram. We isolated approximately 300 endophytic strains from <i>Brassica rapa</i> var. <i>perviridis</i> that had been exposed to different neonicotinoid insecticides. After 14 days of incubation, a bacterial strain, NIT-2, with nitenpyram degradation capability (approximately 65%) was found. <i>Via</i> 16S rRNA gene sequencing, the strain was identified as <i>Bacillus thuringiensis</i>. In addition, metabolites, 2-[<i>N</i>-(6-chloro-3-pyridylmethyl)-<i>N</i>-ethyl]amino-2-methyliminoacetic acid, <i>N</i>-(6-chloro-3-pyridilmethyl)-<i>N</i>-ethyl-<i>N</i>-methylformamidine (CPMF), and <i>N</i>-(6-chloro-3-pyridilmethyl)-<i>N</i>-ethylformamide (CPF), were identified during the degradation. Moreover, CPMF and CPF were further degraded 71% and 18%, respectively by NIT-2. Thus, <i>B. thuringiensis</i> strain NIT-2 is the first reported endophytic bacterium capable of degrading nitenpyram.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-002/figure/advpub_D24-002.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"25 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}