Journal of Pesticide Science最新文献

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Investigation of matrix effects by crops and their compensation methods in a multiresidue analysis using LC-MS/MS. 多残留分析中作物基质效应及其补偿方法的研究。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.J25-05
Arisa Banno
{"title":"Investigation of matrix effects by crops and their compensation methods in a multiresidue analysis using LC-MS/MS.","authors":"Arisa Banno","doi":"10.1584/jpestics.J25-05","DOIUrl":"10.1584/jpestics.J25-05","url":null,"abstract":"<p><p>This study evaluated the matrix effect (ME) in a multiresidue analysis with the modified official Japanese method for agricultural products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In ME comparisons between the vegetable and fruit samples, it was found that more numerous analytes exhibited ion suppression in the vegetable samples than in the fruit samples, and substantial ion enhancement was not observed in most of the analyte-sample combinations. The ME could significantly vary, even within the same commodity, and it was suggested that sampling has greater influence than measurement when there is a wide ME variability. Dilution, the internal standard calibration method and the matrix-matched calibration method are practical countermeasures against MEs, but certain limitations in their applications should be considered. Moreover, a novel cleanup procedure suitable for hydrophilic neonicotinoid pesticides that minimized the usage of expensive internal standard solutions was suggested.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"155-167"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two new asteltoxins produced by solid-state fermentation of Pochonia suchlasporia TAMA 87 and their effects on cell division in sea urchin embryos. 两种新的星毒素由Pochonia suchlasporia TAMA 87固态发酵产生及其对海胆胚胎细胞分裂的影响。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.D25-049
Thi Khanh Ngoc Nguyen, Haruki Kato, Hiroshi Kanzaki, Toru Okuda, Teruhiko Nitoda
{"title":"Two new asteltoxins produced by solid-state fermentation of <i>Pochonia suchlasporia</i> TAMA 87 and their effects on cell division in sea urchin embryos.","authors":"Thi Khanh Ngoc Nguyen, Haruki Kato, Hiroshi Kanzaki, Toru Okuda, Teruhiko Nitoda","doi":"10.1584/jpestics.D25-049","DOIUrl":"10.1584/jpestics.D25-049","url":null,"abstract":"<p><p>New asteltoxins U (<b>1</b>) and V (<b>2</b>) were obtained from the solid-state fermentation of <i>Pochonia suchlasporia</i> TAMA 87. The spectroscopic characterization of <b>1</b> and <b>2</b> revealed that their chemical structures are similar to that of asteltoxin H, except for the modification of the α-pyrone moiety. Specifically, the methyl group on the γ-position of the α-pyrone moiety in asteltoxin H is replaced with a hydroxymethyl group in <b>1</b> and <b>2</b>. In addition, <b>1</b> and <b>2</b> are a pair of isomers that differ in the geometry of the double bond between C-11 and C-12 in the conjugated triene moiety and that contain an all-<i>trans</i> (9<i>E</i>, 11<i>E</i>, 13<i>E</i>) moiety and a 9<i>E</i>, 11<i>Z</i>, 13<i>E</i> conjugated triene moiety, respectively. Compound <b>1</b> showed inhibitory activity toward the first cleavage of sea urchin embryos with a minimum inhibition concentration value of 3.1 µg/mL, whereas compound <b>2</b> did not show inhibitory activity up to a concentration of 25 µg/mL.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"126-131"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insecticide susceptibility of Japanese mason bees (Osmia spp.) versus that of the western honey bee (Apis mellifera). 日本石蜂(Osmia spp.)与西方蜜蜂(Apis mellifera)对杀虫剂的敏感性。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.D25-036
Yugo Seko, Makihiko Ikegami, Yoichi Ishiguri, Tomoyuki Yokoi, Koichi Goka, Yoshiko Sakamoto
{"title":"Insecticide susceptibility of Japanese mason bees (<i>Osmia</i> spp.) <i>versus</i> that of the western honey bee (<i>Apis mellifera</i>).","authors":"Yugo Seko, Makihiko Ikegami, Yoichi Ishiguri, Tomoyuki Yokoi, Koichi Goka, Yoshiko Sakamoto","doi":"10.1584/jpestics.D25-036","DOIUrl":"10.1584/jpestics.D25-036","url":null,"abstract":"<p><p>Acute toxicity of pesticides to wild bee species is inferred by using the LD<sub>50</sub> values of the western honey bee, <i>Apis mellifera</i>, as a standard baseline. However, substantial discrepancies are often reported between the LD<sub>50</sub> values for wild bees and those for <i>A. mellifera</i>. Here, we conducted tests to compare acute toxicity between Japanese mason bees (<i>Osmia</i> spp.) and the western honey bee. For nearly all tested insecticides, the mortality rates of mason bees were equivalent to, or slightly lower than, those of the western honey bee. However, the LD<sub>50</sub> of acetamiprid in male mason bees was approximately five-fold lower than that in workers of the western honey bee. These results suggest that, although the current pesticide risk assessment is generally conservative for Japanese mason bees, continued attention is warranted to ensure that their sensitivity-particularly to acetamiprid-remains within the presumed safety factor.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"148-154"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of isofetamid on SDHI-resistant isolates of Botrytis cinerea. 异fetamid对葡萄孢sdhi耐药菌株的影响。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.D25-053
Yuzuka Abe, Yoshitake Desaki, Nanami Kuwahara, Yohei Fukumori, Shintaro Tsukuda, Kosuke Tsunematsu, Sayaka Komori, Munekazu Ogawa, Satoshi Araki, Shigeru Mitani, Kazumi Suzuki
{"title":"Effect of isofetamid on SDHI-resistant isolates of <i>Botrytis cinerea</i>.","authors":"Yuzuka Abe, Yoshitake Desaki, Nanami Kuwahara, Yohei Fukumori, Shintaro Tsukuda, Kosuke Tsunematsu, Sayaka Komori, Munekazu Ogawa, Satoshi Araki, Shigeru Mitani, Kazumi Suzuki","doi":"10.1584/jpestics.D25-053","DOIUrl":"10.1584/jpestics.D25-053","url":null,"abstract":"<p><p>Tests of sensitivity to isofetamid conducted with <i>Botrytis cinerea</i> isolated from Hokkaido (2011-2016) and other districts in Japan (2017-2018) revealed the absence of isolates with reduced sensitivity. Isolates of <i>B. cinerea</i> with mutation H272Y/R in sdhB, collected in Germany and Japan, resulted in high and moderate resistance to boscalid and many other succinate dehydrogenase inhibitors (SDHIs), respectively. However, these isolates were as sensitive as wild-type isolate to isofetamid. In contrast, isolate BC-50 [P225F] and BC-49 [H272L] caused moderate to high resistance to isofetamid. Considerable differences in mycelial growth, conidiation, conidial germination, and pathogenicity were not observed between these resistant isolates and sensitive isolates. A competitive assay between resistant isolate BC-50 [P225F] and BC-49 [H272L] and B05.10 [wild type], however, revealed the disappearance of resistant isolates after several generations of mixed incubation in <i>in vitro</i> and <i>in vivo</i> conditions.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"141-147"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accumulation of phytoalexins and alteration in plant hormone profiles in rice leaves following sap sucking by the brown planthopper and green rice leafhopper. 褐飞虱和绿飞虱吸液后水稻叶片中植物抗毒素的积累和植物激素谱的变化。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.D25-047
Keisuke Kariya, Haruka Mori, Takanori Yoshikawa, Masayoshi Teraishi, Kotomi Ueno, Atsushi Ishihara, Shin-Ichi Tebayashi
{"title":"Accumulation of phytoalexins and alteration in plant hormone profiles in rice leaves following sap sucking by the brown planthopper and green rice leafhopper.","authors":"Keisuke Kariya, Haruka Mori, Takanori Yoshikawa, Masayoshi Teraishi, Kotomi Ueno, Atsushi Ishihara, Shin-Ichi Tebayashi","doi":"10.1584/jpestics.D25-047","DOIUrl":"10.1584/jpestics.D25-047","url":null,"abstract":"<p><p>This study investigated the accumulation of cultivar-specific phytoalexins in rice-abietoryzins and oryzalactone-as well as known phytoalexins-momilactone A, phytocassane A, and oryzalexins A and S-in response to sap sucking by the brown planthopper (BPH) and the green rice leafhopper (GRL). The analysis revealed that BPH feeding induced abietoryzin accumulation in a cultivar known to accumulate these phytoalexins following fungal infection. Additionally, oryzalactone and oryzalexin S accumulated after insect attack in specific cultivars, unlike oryzalexin A. Plant hormone profiling revealed distinct associations: momilactone A accumulation was strongly correlated with salicylic acid (SA); phytocassane A with jasmonic acid, jasmonic acid-isoleucine, isopentenyladenine, and abscisic acid (ABA); and oryzalactone/oryzalexin S with both SA and ABA. These findings suggest that distinct signaling pathways regulate the induction of specific phytoalexins. The analysis of phenolic metabolites showed only minor changes in response to insect feeding. The findings highlight the broad inducibility of phytoalexins in rice and reveal the complex hormonal regulation underlying their biosynthesis during herbivore attack.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"117-125"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Degradation of the anilide fungicide inpyrfluxam in illuminated water-sediment systems. 苯胺类杀菌剂吡fluxam在光照水-沉积物系统中的降解。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-11-20 DOI: 10.1584/jpestics.D25-038
Takeshi Adachi, Terumi Sugano, Kenji Okuda, Yusuke Suzuki, Takuo Fujisawa
{"title":"Degradation of the anilide fungicide inpyrfluxam in illuminated water-sediment systems.","authors":"Takeshi Adachi, Terumi Sugano, Kenji Okuda, Yusuke Suzuki, Takuo Fujisawa","doi":"10.1584/jpestics.D25-038","DOIUrl":"10.1584/jpestics.D25-038","url":null,"abstract":"<p><p>The degradation behavior of inpyrfluxam (<b>1</b>) was investigated in aerobic aquatic water-sediment systems exposed to continuous artificial sunlight (λ>290 nm). Under irradiation in the presence of sediment, <b>1</b> preferentially underwent oxidation at the 1'- and 3'-positions of the indane ring, followed by cleavage of the amide linkage with the half-life of 16-18 days, and finally mineralized to carbon dioxide or exhibited extremely strong adsorption to sediment. Especially, as compared to systems kept in darkness, carboxylation at the 1'-position of the indane ring was remarkably accelerated in illuminated water-sediment systems and the aqueous photodegradation study due to the presence of photosynthetic microorganisms in the sediment soil. No significant degradation products were observed in the water-sediment in darkness and in the sterilized water-sediment under irradiation throughout the study. The fate of <b>1</b> and its degradation products in illuminated water-sediment systems was considered to better reflect realistic conditions, as it accounts for various effects attributed to sunlight, such as the presence of photosynthetic microorganisms.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 4","pages":"132-140"},"PeriodicalIF":1.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis and biological evaluation of trifluoromethyl-containing auxin derivatives. 含三氟甲基生长素衍生物的合成及生物学评价。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-08-20 DOI: 10.1584/jpestics.D25-026
Mitsuaki Yamashita, Fuki Saneoka, Aozora Sano, Towa Arakawa, Akira Iida
{"title":"Synthesis and biological evaluation of trifluoromethyl-containing auxin derivatives.","authors":"Mitsuaki Yamashita, Fuki Saneoka, Aozora Sano, Towa Arakawa, Akira Iida","doi":"10.1584/jpestics.D25-026","DOIUrl":"10.1584/jpestics.D25-026","url":null,"abstract":"<p><p>This study focused on the chemical synthesis of auxin analogs, wherein a trifluoromethyl group was introduced near the carboxyl group in the side chain of natural and synthetic auxins, including IAA, NAA, IBA, 2,4-D, and 4-Cl-IAA. The effects of these synthetic compounds and natural auxins on plant growth regulation and callus growth were evaluated. In experiments with black gram, CF<sub>3</sub>-IAA and 4-Cl-CF<sub>3</sub>-IAA exhibited comparable effects to the parent compound, IAA. Meanwhile, CF<sub>3</sub>-NAA, CF<sub>3</sub>-2,4-D, CF<sub>3</sub>-IBA-1, and CF<sub>3</sub>-IBA-2 displayed effects that differed considerably from those of their respective parent auxins. In experiments with lettuce, CF<sub>3</sub>-IAA, 4-Cl-CF<sub>3</sub>-IAA, CF<sub>3</sub>-NAA, CF<sub>3</sub>-2,4-D, and CF<sub>3</sub>-IBA-1 showed effects comparable to the corresponding parent auxins. However, at low concentrations, these analogs induced hypocotyl and root elongations, a response distinct from that observed with their parent compounds. Furthermore, CF<sub>3</sub>-IBA-2 considerably promoted hypocotyl and root elongations across all concentrations relative to the control. The addition of synthetic compounds to callus cultures revealed that CF<sub>3</sub>-IAA, 4-Cl-CF<sub>3</sub>-IAA, CF<sub>3</sub>-NAA, and CF<sub>3</sub>-2,4-D promoted callus proliferation, whereas CF<sub>3</sub>-IBA-1 and CF<sub>3</sub>-IBA-2 did not enhance callus growth.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 3","pages":"64-73"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a novel acaricide, acynonapyr. 一种新型杀螨剂的研制。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-08-20 DOI: 10.1584/jpestics.J25-04
Jun Takahashi, Masahiro Kawaguchi, Koichi Hirata, Keiji Koizumi
{"title":"Development of a novel acaricide, acynonapyr.","authors":"Jun Takahashi, Masahiro Kawaguchi, Koichi Hirata, Keiji Koizumi","doi":"10.1584/jpestics.J25-04","DOIUrl":"10.1584/jpestics.J25-04","url":null,"abstract":"<p><p>Acynonapyr is a novel acaricide developed by Nippon Soda Co., Ltd. It contains a unique azabicyclic ring and oxyamine structure and represents the first agricultural chemical that targets calcium-activated potassium channels, classified as Group 33 in the IRAC Mode of Action Classification. Acynonapyr exhibits high selectivity against spider mites across all developmental stages and has minimal impact on beneficial insects and natural enemies, rendering it suitable for Integrated Pest Management systems. The compound acts by inhibiting potassium ion flow through K<sub>Ca</sub>2 channels in spider mites, leading to neurological symptoms such as convulsions and impaired mobility and ultimately resulting in mortality. Electrophysiological studies have demonstrated that acynonapyr effectively blocks <i>Tetranychus urticae</i> calcium-activated potassium channels. Importantly, acynonapyr shows little activity against mammalian calcium-activated potassium channels, contributing to its favorable safety profile. The compound shows efficacy against acaricide-resistant spider mite populations, providing a useful tool for pesticide resistance management.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 3","pages":"102-104"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioorganic chemistry of natural products that control plant pathogens. 控制植物病原体的天然产物的生物有机化学。
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-08-20 DOI: 10.1584/jpestics.J25-03
Arata Yajima
{"title":"Bioorganic chemistry of natural products that control plant pathogens.","authors":"Arata Yajima","doi":"10.1584/jpestics.J25-03","DOIUrl":"10.1584/jpestics.J25-03","url":null,"abstract":"<p><p>Developing new agrochemicals is essential for sustainable agriculture and global food security. Our group focused on natural products that control plant pathogens, conducting synthetic research across three key areas of interest: antimicrobial compounds, phytoalexins, and microbial signaling molecules. We established new methods for producing chiral allylic alcohols as useful synthetic intermediates for natural product synthesis <i>via</i> the enantioselective synthesis of antimicrobial agents such as peniciaculins. In the phytoalexin research, the synthesis of biosynthetic intermediates enabled the elucidation of enzyme functions in terms of their biosynthesis and the confirmation of absolute configurations, deepening our understanding of plant defense systems. Furthermore, the total synthesis and biosynthetic studies of <i>Phytophthora</i> mating hormones revealed a unique chemical relay system regulating sexual reproduction. These findings emphasize the importance of synthetic chemistry in advancing natural product research and offer new strategies for crop protection. Our interdisciplinary approach paves the way for future innovations in combating agricultural pests and diseases.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 3","pages":"87-95"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of succinate dehydrogenase inhibitor-resistance mutations, T78I, N85K/S, and H151R, in the SdhC gene in the tomato leaf mold pathogen, Passalora fulva. 番茄叶霉病病原菌SdhC基因T78I、N85K/S和H151R抗性突变的鉴定
IF 1.8 4区 农林科学
Journal of Pesticide Science Pub Date : 2025-08-20 DOI: 10.1584/jpestics.D25-007
Kenshi Hirai, Ryouko Satake, Hideki Watanabe, Kaori Nakajima, Taku Kawakami, Fumiyasu Fukumori, Makoto Fujimura, Akihiko Ichiishi
{"title":"Identification of succinate dehydrogenase inhibitor-resistance mutations, T78I, N85K/S, and H151R, in the <i>SdhC</i> gene in the tomato leaf mold pathogen, <i>Passalora fulva</i>.","authors":"Kenshi Hirai, Ryouko Satake, Hideki Watanabe, Kaori Nakajima, Taku Kawakami, Fumiyasu Fukumori, Makoto Fujimura, Akihiko Ichiishi","doi":"10.1584/jpestics.D25-007","DOIUrl":"10.1584/jpestics.D25-007","url":null,"abstract":"<p><p>Tomato leaf mold caused by <i>Passalora fulva</i> is a significant disease in tomato production. We isolated several types of boscalid-resistant isolates in the Gifu and Mie Prefectures of Japan. Sequencing analysis of succinate dehydrogenase (Sdh) subunits B, C, and D genes strongly indicated that four amino acid substitutions-T78I, N85K, N85S, and H151R in SdhC-conferred boscalid resistance. We conducted SNP assays to detect each mutation using qPCR techniques and revealed that all 35 resistant isolates had one of these mutations in the <i>SdhC.</i> Among the four resistance types, N85K isolates exhibited the highest, N85S isolates showed the lowest, and T78I and H151R isolates displayed moderate resistance to boscalid. These mutations also conferred cross-resistance to other succinate dehydrogenase inhibitor (SDHI) fungicides, including penthiopyrad, pyraziflumid, fluopyram, and isofetamid. A predicted SdhC protein structure, created by I-TASSER, suggests that the amino acid at position 151 is located close to those of positions 78 and 85, likely forming the SDHI-binding pocket of the protein.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 3","pages":"55-63"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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