Journal of Pesticide Science最新文献

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Comparative evaluation of trimethylated α-, β-, and γ-cyclodextrins as optimal dispersants for ready biodegradability testing of poorly water-soluble substances.
IF 1.5 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-11-20 DOI: 10.1584/jpestics.D24-015
Yoshinari Takano, Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Makiko Mukumoto, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
{"title":"Comparative evaluation of trimethylated α-, β-, and γ-cyclodextrins as optimal dispersants for ready biodegradability testing of poorly water-soluble substances.","authors":"Yoshinari Takano, Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Makiko Mukumoto, Keisei Sowa, Yuki Kitazumi, Osamu Shirai","doi":"10.1584/jpestics.D24-015","DOIUrl":"10.1584/jpestics.D24-015","url":null,"abstract":"<p><p>We investigated whether various modified cyclodextrins (CDs) and emulsifiers could be applied as dispersing agents in ready biodegradability tests of poorly water-soluble substances. Trimethylated α-, β-, and γ-CDs and partially methylated β-CD were not biodegraded in the test period but accelerated the biodegradation of octabenzone and anthraquinone. The process by which trimethylated α-, β-, and γ-CDs enhance the biodegradation of test substances has been partially uncovered. These CDs create inclusion complexes with the substances, which then coalesce into larger aggregates. These aggregates disperse throughout the testing medium and attach to clusters of activated sludge, known as flocs. This close contact with the sludge speeds up the breakdown of the hydrophobic substances being tested.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"210-223"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coprinolide, a novel antifungal tricyclic polyketide with a rare furanone-fused chromene skeleton isolated from the mushroom Coprinus comatus.
IF 1.5 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-11-20 DOI: 10.1584/jpestics.D24-040
Enrico M Cabutaje, Kota Seki, Motoichiro Kodama, Tsutomu Arie, Kotomi Ueno, Thomas Edison E Dela Cruz, Atsushi Ishihara
{"title":"Coprinolide, a novel antifungal tricyclic polyketide with a rare furanone-fused chromene skeleton isolated from the mushroom <i>Coprinus comatus</i>.","authors":"Enrico M Cabutaje, Kota Seki, Motoichiro Kodama, Tsutomu Arie, Kotomi Ueno, Thomas Edison E Dela Cruz, Atsushi Ishihara","doi":"10.1584/jpestics.D24-040","DOIUrl":"10.1584/jpestics.D24-040","url":null,"abstract":"<p><p>A search for antifungal compounds from the mushroom <i>Coprinus comatus</i> using a bioassay-guided chromatographic fractionation approach led to the discovery of a novel polyketide harboring a rare 3,3a,9,9a-tetrahydro-1<i>H</i>-furo[3,4-<i>b</i>]chromen-1-one skeleton. The novel compound was named coprinolide. The inhibitory activity and fungicidal potential of coprinolide were evaluated against five economically important plant-pathogenic fungi. Coprinolide showed inhibitory effects on conidial germination and germ tube elongation of all tested fungi. The strongest effect was observed for <i>Colletotrichum orbiculare</i> with half-maximal inhibitory concentrations of 7.1 ppm and 8.2 ppm for conidial germination and germ tube elongation, respectively. Furthermore, coprinolide exhibited fungicidal activity against the tested fungi by inhibiting conidial germination to conidial death as confirmed by fluorescence microscopy using fluorescein diacetate and propidium iodide. These findings showed the potential of the mushroom as a source of a novel bioactive compound with promising agricultural application as an antifungal agent against different plant-pathogenic fungi.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"243-254"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A suitable solvent for adsorption of poorly water-soluble substances onto silica gel in a ready biodegradability test.
IF 1.5 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-11-20 DOI: 10.1584/jpestics.D24-016
Yoshinari Takano, Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Makiko Mukumoto, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
{"title":"A suitable solvent for adsorption of poorly water-soluble substances onto silica gel in a ready biodegradability test.","authors":"Yoshinari Takano, Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Makiko Mukumoto, Keisei Sowa, Yuki Kitazumi, Osamu Shirai","doi":"10.1584/jpestics.D24-016","DOIUrl":"10.1584/jpestics.D24-016","url":null,"abstract":"<p><p>When a test substance is poorly water-soluble, it can be adsorbed onto silica gel to facilitate dispersibility in a ready biodegradability test. To uniformly adsorb the test substance onto silica gel, the substance is dissolved in a solvent and then mixed with the silica gel. It is desirable for the solvent to completely evaporate afterward. In this study, we identified <i>n</i>-hexane as a suitable solvent for this purpose. Furthermore, through fluorescence observation, we revealed that the test substance adsorbed onto the silica gel adhered to activated sludge flocs. This is thought to improve contact between the test substance and microorganisms, thereby accelerating biodegradation.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"271-276"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design, synthesis, and biological evaluation of insect hormone agonists.
IF 1.5 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-11-20 DOI: 10.1584/jpestics.J24-02
Taiyo Yokoi
{"title":"Design, synthesis, and biological evaluation of insect hormone agonists.","authors":"Taiyo Yokoi","doi":"10.1584/jpestics.J24-02","DOIUrl":"10.1584/jpestics.J24-02","url":null,"abstract":"<p><p>Agonists of insect hormones, namely molting hormone (MH) and juvenile hormone (JH), disrupt the normal growth of insects and can be employed as insecticides that are harmless to vertebrates. In this study, a series of experiments and computational analyses were conducted to rationally design novel insect hormone agonists. Syntheses and quantitative structure-activity relationship (QSAR) analyses of two MH agonist chemotypes, imidazothiadiazoles and tetrahydroquinolines, revealed that the structural factors important for the ligand-receptor interactions are significantly different between these chemotypes. On the other hand, a virtual screening cascade combining ligand- and structure-based methods identified a piperazine derivative as a novel JH agonist. The results obtained in this study will be useful for the future development of novel insect growth regulators.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"303-310"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves 吡蚜酮对小麦叶片上禾谷蓝霉菌感染过程和细胞学特征的影响
IF 2.4 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-08-27 DOI: 10.1584/jpestics.d24-005
Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani
{"title":"Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves","authors":"Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani","doi":"10.1584/jpestics.d24-005","DOIUrl":"https://doi.org/10.1584/jpestics.d24-005","url":null,"abstract":"</p><p>Pyriofenone demonstrates outstanding efficacy in controlling powdery mildew. We investigated the impact of pyriofenone on the infection processes and cytological features of <i>Blumeria graminis</i> f. sp. <i>tritici</i> on wheat leaves. The preventive application of pyriofenone before inoculation did not inhibit conidial germination but effectively suppressed both appressorial and haustorial formation. Notably, haustorial formation was effectively inhibited, resulting in the complete suppression of successive lesion development and sporulation. Curative application of pyriofenone after inoculation also inhibited lesion expansion and sporulation. Furthermore, it had considerable impact on the morphogenesis of powdery mildew fungus. We observed multi-formed secondary appressoria, shrunken or bifurcated hyphae, abnormal conidiophores, and clubbed conidia-like structures. Subsequently, we employed a histochemical approach to analyze the localization of essential components for the polar growth of fungal hyphae. Pyriofenone induced mislocalization of the actin cytoskeleton, β-glucan and cytoplasmic vesicles, although it did not affect tubulin orientation.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-005/figure/advpub_D24-005.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"8 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates. 合成灼烧烯 A 衍生物并对其进行生物学评价,从而发现新型候选杀真菌剂。
IF 1.5 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-08-20 DOI: 10.1584/jpestics.D24-014
Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Masako Honsho, Sota Negami, Sota Honma, Katsuyuki Sakai, Toshiyuki Tokiwa, Hiroki Kojima, Kenichi Nonaka, Shin-Ichi Fuji, Takumi Chinen, Takeo Usui, Yukihiro Asami
{"title":"Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates.","authors":"Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Masako Honsho, Sota Negami, Sota Honma, Katsuyuki Sakai, Toshiyuki Tokiwa, Hiroki Kojima, Kenichi Nonaka, Shin-Ichi Fuji, Takumi Chinen, Takeo Usui, Yukihiro Asami","doi":"10.1584/jpestics.D24-014","DOIUrl":"https://doi.org/10.1584/jpestics.D24-014","url":null,"abstract":"<p><p>An antifungal polyene-decalin polyketide natural product, burnettiene A (<b>1</b>) has been re-discovered from the culture broth of <i>Lecanicillium primulinum</i> (current name: <i>Flavocillium primulinum</i>) FKI-6715 strain utilizing our original multidrug-sensitive yeast system. This polyene-decalin polyketide natural product was originally isolated from <i>Aspergillus burnettii</i>. The antifungal activity of <b>1</b> against <i>Candida albicans</i> has been reported. However, only one fungal species for the antifungal activity of <b>1</b> has been revealed, and details of the antifungal activity against other pathogenic fungus remain unknown. After extensive screening for antifungal activity, we found that <b>1</b> exhibits broad antifungal activity against pathogenic plant fungi, including <i>Colletotrichum gloeosporioides</i>, <i>Botrytis cinerea</i>, <i>Pyricularia oryzae</i>, <i>Leptosphaeria maculans</i>, and <i>Rhizoctonia solani</i>. Furthermore, we synthesized 12 derivatives from <b>1</b> and evaluated their antifungal activity to reveal the detailed structure-activity relationship. The methyl ester derivative showed antifungal activity against <i>Saccharomyces cerevisiae</i> 12geneΔ0HSR-iERG6 100-fold more potent than that of <b>1</b>. Our research indicates that <b>1</b> would be a promising natural product as a new fungicidal candidate and the methyl ester derivative especially has great potential.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 3","pages":"159-167"},"PeriodicalIF":1.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana 拟南芥通过绞股蓝内酯介导的免疫启动增强对灰霉病的抗病性
IF 2.4 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-08-09 DOI: 10.1584/jpestics.d24-019
Moeka Fujita, Tomoya Tanaka, Miyuki Kusajima, Kengo Inoshima, Futo Narita, Hidemitsu Nakamura, Tadao Asami, Akiko Maruyama-Nakashita, Hideo Nakashita
{"title":"Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana","authors":"Moeka Fujita, Tomoya Tanaka, Miyuki Kusajima, Kengo Inoshima, Futo Narita, Hidemitsu Nakamura, Tadao Asami, Akiko Maruyama-Nakashita, Hideo Nakashita","doi":"10.1584/jpestics.d24-019","DOIUrl":"https://doi.org/10.1584/jpestics.d24-019","url":null,"abstract":"</p><p>Strigolactones (SLs) are a class of plant hormones that play several roles in plants, such as suppressing shoot branching and promoting arbuscular mycorrhizal symbiosis. The positive regulation of plant disease resistance by SLs has recently been demonstrated by analyses using SL-related mutants. In Arabidopsis, SL-mediated signaling has been reported to modulate salicylic acid-mediated disease resistance, in which the priming of plant immunity plays an important role. In this study, we analyzed the effect of the synthetic SL analogue <i>rac</i>-GR24 on resistance against necrotrophic pathogen <i>Botrytis cinerea</i>. In <i>rac</i>-GR24-treated plants, disease resistance against <i>B. cinerea</i> was enhanced in an ethylene- and camalexin-dependent manners. Expression of the ethylene-related genes and the camalexin biosynthetic gene and camalexin accumulation after pathogen infection were enhanced by immune priming in <i>rac</i>-GR24-treated plants. These suggest that SL-mediated immune priming is effective for many types of resistance mechanisms in plant self-defense systems.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-019/figure/advpub_D24-019.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"107 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry 使用日本官方分析方法 QuEChERS 和改良 QuEChERS 结合同位素稀释质谱法,可靠定量糙米样品中的有机磷农药,以进行能力验证
IF 2.4 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-07-24 DOI: 10.1584/jpestics.d24-021
Takamitsu Otake, Keisuke Nakamura, Naoyuki Hirabayashi, Takaho Watanabe
{"title":"A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry","authors":"Takamitsu Otake, Keisuke Nakamura, Naoyuki Hirabayashi, Takaho Watanabe","doi":"10.1584/jpestics.d24-021","DOIUrl":"https://doi.org/10.1584/jpestics.d24-021","url":null,"abstract":"</p><p>The objective of the present study is to provide reliable concentration values as assigned values for target pesticides in brown rice samples used in proficiency testing (PT) organized by the Hatano Research Institute (HRI). The test samples for PT were prepared by immersing brown rice in the pesticide solution and using a spray dryer by the HRI. Homogeneity and stability assessments were performed for PT samples, and the relative uncertainties due to inhomogeneity and instability were 0.58 %–0.78 % and 0 %–0.96 %, respectively. Quantification for the assigned values of target pesticides by the National Metrology Institute of Japan (NMIJ) was carried out using the multiple analytical methods including Japanese official analytical method, QuEChERS, and modified QuEChERS, which were combined with isotope dilution mass spectrometry, to ensure the reliability of the analytical results. The NMIJ assigned values were 0.065±0.004 mg/kg for chlorpyrifos, 0.217±0.012 mg/kg for diazinon, 0.138±0.008 mg/kg for fenitrothion, and 0.138±0.008 mg/kg for malathion.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-021/figure/advpub_D24-021.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"95 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS 细菌杀虫剂:作用机理、食品污染的可能性以及使用 MS 进行残留分析
IF 2.4 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-07-19 DOI: 10.1584/jpestics.d24-006
Hiroto Tamura
{"title":"Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS","authors":"Hiroto Tamura","doi":"10.1584/jpestics.d24-006","DOIUrl":"https://doi.org/10.1584/jpestics.d24-006","url":null,"abstract":"</p><p>As Sustainable Development Goals (SDGs) and the realities of climate change become widely accepted around the world, the next-generation of integrated pest management will become even more important for establishing a sustainable food production system. To meet the current challenge of food security and climate change, biological control has been developed as one sustainable crop protection technology. However, most registered bacteria are ubiquitous soil-borne bacteria that are closely related to food poisoning and spoilage bacteria. Therefore, this review outlined (1) the mechanism of action of bacterial pesticides, (2) potential concerns about secondary contamination sources associated with past food contamination, and, as a prospective solution, focused on (3) principles and methods of bacterial identification, and (4) the possibility of identifying residual bacteria based on mass spectrometry.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-006/figure/advpub_D24-006.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"10 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141739968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-throughput screening for novel Bacillus thuringiensis insecticidal proteins revealed evidence that the bacterium exchanges Domain III to enhance its insecticidal activity 高通量筛选新型苏云金芽孢杆菌杀虫蛋白的结果表明,苏云金芽孢杆菌通过交换领域 III 来增强其杀虫活性
IF 2.4 4区 农林科学
Journal of Pesticide Science Pub Date : 2024-07-10 DOI: 10.1584/jpestics.d24-011
Ruth Cong, Jing-Tong Hou, Takashi Yamamoto
{"title":"High-throughput screening for novel Bacillus thuringiensis insecticidal proteins revealed evidence that the bacterium exchanges Domain III to enhance its insecticidal activity","authors":"Ruth Cong, Jing-Tong Hou, Takashi Yamamoto","doi":"10.1584/jpestics.d24-011","DOIUrl":"https://doi.org/10.1584/jpestics.d24-011","url":null,"abstract":"</p><p>Approximately 3000 <i>Bacillus thuringiensis</i> (Bt) isolates were screened to discover novel three-domain (3D) Cry proteins active against <i>Helicoverpa zea</i> (corn earworm). From 400 active isolates found during the primary screening, Cry1Ac and Cry2A, which are known to be active against <i>H. zea</i>, were removed using multiplex-primer PCR and high-throughput column chromatography. This process reduced the number of active cultures to 48. DNA segments encoding Domain III of these 48 cultures were amplified by PCR and sequenced. Sequencing revealed two novel Cry1B-type Domain IIIs. Further sequencing of the flanking regions of these domains revealed that one was part of Cry1Bj (GenBank: KT952325). However, the other Domain III lacked Domains I and II. Instead, this Domain III was associated with two open reading frames, ORF1 and ORF2. ORF1 was identified as an ATP-binding protein, and ORF2 as an ATPase, suggesting that Bt exchanges Domain III among homologous Cry proteins.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-011/figure/advpub_D24-011.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"5 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141584872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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