Menghan Li, Longfei Jin, Bin Liu, Yifang Qian, Haofu Hu, Xin Wang, Yang Zhang, Wenping Xu
{"title":"Advantages of ethylene glycol diacetate as a potential solvent for herbicide formulations.","authors":"Menghan Li, Longfei Jin, Bin Liu, Yifang Qian, Haofu Hu, Xin Wang, Yang Zhang, Wenping Xu","doi":"10.1584/jpestics.D24-051","DOIUrl":"10.1584/jpestics.D24-051","url":null,"abstract":"<p><p>Emulsifiable concentrates and oil suspensions are typical pesticide formulations. Many pesticides on the market use aromatic hydrocarbons as solvents. However, studies have revealed their potential risks to humans and the environment. Ethylene glycol diacetate (EGDA) is a low-toxicity and eco-friendly solvent with low utilization in pesticides. This study explores EGDA's potential to replace xylene. Results indicate that EGDA formulations enhance droplet adhesion to leaves, boosting pesticide efficiency. They exhibit lower surface tension and contact angles, with a 24%-40% increase in leaf retention. Bioassays show that 15% cyhalofop-butyl EC and 10% nicosulfuron OF with EGDA offer weed control that is superior to xylene-based formulations by 9.1%-30.5% in greenhouses and 4.8%-6.7% in fields. Xylene preparations are 2-3 times more cytotoxic to human bronchial cells than EGDA-based ones. Thus, EGDA is a promising pesticide solvent, outperforming traditional aromatic solvents in environmental friendliness and reducing adverse effects.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 2","pages":"31-39"},"PeriodicalIF":1.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological efficacy of polyoxin D in crop protection.","authors":"Kyuya Suda, Shin-Ichiro Kochi","doi":"10.1584/jpestics.D24-055","DOIUrl":"10.1584/jpestics.D24-055","url":null,"abstract":"<p><p>Polyoxin D is purified from the culture broth of actinomycetes and used as an agricultural fungicide. This compound shows antifungal activity against various plant pathogenic fungi, especially <i>Rhizoctonia solani</i>, by inhibiting germination, hyphal growth, and sporulation of the fungi. Its mode of action is thought to be <i>via</i> the competitive inhibition of chitin synthase, which causes incomplete cell wall formation and swelling in germ tubes, hyphae and septa. This action is unique and results in the suppression of diseases in various crops through fungistatic rather than fungicidal activity. Although the polyoxin complex shows not only fungicidal but also insecticidal activity, polyoxin D has less activity against insects, no negative impacts on the environment and is suitable for organic agriculture. Polyoxin D has the potential to control soil-borne and post-harvest diseases and to inhibit mycotoxin production <i>via</i> a new mode of action.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 2","pages":"19-30"},"PeriodicalIF":1.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessing α-galactosidase OmAGAL2 inhibitors and their impact on germination and sugar profiles in <i>Orobanche minor</i>.","authors":"Masahiro Onitsuka, Takatoshi Wakabayashi, Takumi Ogawa, Yukihiro Sugimoto, Daisaku Ohta, Atsushi Okazawa","doi":"10.1584/jpestics.D25-005","DOIUrl":"10.1584/jpestics.D25-005","url":null,"abstract":"<p><p>Root parasitic weeds from the <i>Orobanche</i> genus significantly damage crop production in African and European countries. Previous studies identified the metabolism of planteose, a storage trisaccharide in root parasitic weeds, as a potential control target. In <i>Orobanche minor</i>, α-galactosidase OmAGAL2 hydrolyzes planteose into sucrose upon perceiving germination stimulant strigolactones. Subsequently, invertases break down sucrose into glucose and fructose, essential for germination. This study screened chemical libraries to identify inhibitors against OmAGAL2-mCherry, secreted from transgenic tobacco BY-2 cells. Two inhibitors, 82-G8 and 85-B10, which significantly reduced the OmAGAL2 activity to less than 70% of the control, were evaluated for their impact on <i>O. minor</i> germination and sugar profiles. Results showed that OmAGAL2 inhibitors suppressed <i>O. minor</i> radicle elongation by inhibiting planteose metabolism, with effects more pronounced when applied at the start of conditioning rather than during germination stimulation. Further structural optimization could yield a novel class of chemicals for controlling <i>Orobanche</i> spp.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 2","pages":"40-46"},"PeriodicalIF":1.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synthesis and herbicidal activity of C5-substituted cinmethylin analogs.","authors":"Narihito Ogawa, Kakeru Hasegawa, Masaru Kawakami, Chihiro Uematsu, Tatsuya Hirano, Yusuke Mitani, Akihito Ootaka","doi":"10.1584/jpestics.D24-056","DOIUrl":"10.1584/jpestics.D24-056","url":null,"abstract":"<p><p>We investigated the synthesis and herbicidal activity of C5-substituted cinmethylin analogs. For the benzyl ether at the C2 position, we found that electron-withdrawing groups, such as halogen groups on the benzene ring have high herbicidal activity. Analogues with ketone, fluorine, and methoxy groups at the C5 position also showed excellent herbicidal activity.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 2","pages":"47-54"},"PeriodicalIF":1.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ovicidal activity of adulticidal insecticides against the invasive wood borer <i>Aromia bungii</i>.","authors":"Yuichi Yamamoto, Shuji Kaneko","doi":"10.1584/jpestics.D24-050","DOIUrl":"10.1584/jpestics.D24-050","url":null,"abstract":"<p><p>The development of preventive methods against the invasive wood-boring beetle <i>Aromia bungii</i>, which infests Rosaceae trees, is required in Japan. We investigated the effect on eggs of several commercial insecticides whose active ingredients are registered to kill <i>A. bungii</i> adults or larvae by tree injection or spray. The ovicidal activity was evaluated using the corrected egg hatch rates based on the control (distilled water) after dipping pieces of cardboard, on which eggs were deposited, in liquid insecticide solutions for 10 sec. The egg-dipping tests revealed highly effective ovicidal activity in the following insecticides: more than 8000 ppm for fenitrothion, 1000 ppm for acetamiprid, 400 ppm for clothianidin, and 500 ppm for dinotefuran (corrected egg-hatch rates of approximately less than 5%). In terms of current registration in Japan, only fenitrothion (SUMIPAIN emulsion) and acetamiprid (MATSUGREEN liquid solution 2) by trunk spraying during the <i>A. bungii</i> reproductive period are adulticidal insecticide candidates with preventive potential through incidental ovicidal activity.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 1","pages":"1-8"},"PeriodicalIF":1.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research and development of an insecticide, afidopyropen.","authors":"Ryo Horikoshi, Kimihiko Goto, Masaaki Mitomi, Toshiaki Sunazuka, Satoshi Ōmura","doi":"10.1584/jpestics.J25-01","DOIUrl":"10.1584/jpestics.J25-01","url":null,"abstract":"","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 1","pages":"14-17"},"PeriodicalIF":1.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synthesis of <i>N</i>-alkylated octopamine derivatives and their interaction with octopamine receptor BmOAR1.","authors":"Kenji Oshima, Ryunosuke Yamamoto, Haruna Yamasaki, Maki Katayama, Keita Noda, Tomohiro Oishi, Hiroto Ohta","doi":"10.1584/jpestics.D24-054","DOIUrl":"10.1584/jpestics.D24-054","url":null,"abstract":"<p><p>A series of <i>N</i>-alkylated octopamine derivatives was synthesized, and the structure-activity relationships of these derivatives with the silkworm <i>Bombyx mori</i> octopamine receptor BmOAR1 were evaluated using a secreted placental alkaline phosphatase reporter assay system. The <i>N</i>-alkyl moiety on the ligand affected the intensity of the agonist activity in the order: CH<sub>3</sub>>(H)>C<sub>2</sub>H<sub>5</sub>. Although linear alkyl chains of C3 or higher did not exhibit any activity, the fixed C3 alkyl group forming a pyrrolidine ring showed significant activity. These results suggest that BmOAR1 has a relatively small space around the amine-binding site, and the alkyl part constituting the cyclic amine could exert the same effect as the small alkyl group.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"50 1","pages":"9-13"},"PeriodicalIF":1.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haruka Takeuchi, Masahiro Nomura, Ryo Horikoshi, Shu Aasano, Takaaki Miyake, Takeru Kobayashi, Masatoshi Murai, Ying-Ju Chen, Fergus Earley, John Mina, Andrew Crossthwaite
{"title":"Flometoquin, a novel insecticide, acts on mitochondrial complex III as a Q<sub>i</sub> inhibitor.","authors":"Haruka Takeuchi, Masahiro Nomura, Ryo Horikoshi, Shu Aasano, Takaaki Miyake, Takeru Kobayashi, Masatoshi Murai, Ying-Ju Chen, Fergus Earley, John Mina, Andrew Crossthwaite","doi":"10.1584/jpestics.D24-008","DOIUrl":"10.1584/jpestics.D24-008","url":null,"abstract":"<p><p>Flometoquin (FLO) is a novel quinoline-type insecticide that elicits a quick knock-down effect against target pests; however, its mode of action (MoA) remains unknown. In this study, we investigated its MoA systematically, using varying biochemical techniques. Since FLO-treated insects exhibited symptoms similar to those induced by respiratory inhibitors, we examined the effect of FLO on respiratory enzyme complexes using mitochondria isolated from different insects (housefly, diamondback moth, and western flower thrips). We found that FLO itself is not active; however, its deacylated metabolite, FloMet, specifically inhibits the activity of ubiquinol-cytochrome <i>c</i> oxidoreductase (complex III) in mitochondria at the nM level. Ligand binding assays and monitoring the reduction kinetics of cytochrome hemes <i>b</i> and <i>c</i> <sub>1</sub> clearly revealed that FloMet inhibits complex III by binding to the Q<sub>i</sub> site.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"203-209"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Discovery of anti-phytopathogenic fungal activity of a new type of (<i>S</i>)-coumarin bearing a phenylpropanoid unit at the 3-position.","authors":"Hazna Sartiva, Hisashi Nishiwaki, Koichi Akiyama, Satoshi Yamauchi","doi":"10.1584/jpestics.D24-038","DOIUrl":"10.1584/jpestics.D24-038","url":null,"abstract":"<p><p>The enantiospecific anti-phytopathogenic fungal activity of a new type of coumarin bearing a phenylpropanoid unit at the 3-position was found. (<i>S</i>)-3-[1-Methoxy-3-(4-methoxyphenyl)prop-2-yl]coumarin ((<i>S</i>)-<b>5</b>: EC<sub>50</sub>=16.5 µM) was 30 times more effective than the (<i>R</i>)-form against the <i>Alternaria alternata</i> Japanese pear pathotype. Derivatives bearing different substituents on the 7'-aromatic ring and the coumarin ring were synthesized to discover the more potent compounds. The 3'-CF<sub>3</sub> and 4'-CF<sub>3</sub> derivatives, <b>39</b> and <b>40</b>, respectively, had the lowest EC<sub>50</sub> values (1-2 µM) in this project, suggesting that the size of the electron-withdrawing and hydrophobic substituents at these positions gave an advantage. On the coumarin ring, the presence of the OCH<sub>3</sub> or CH<sub>3</sub> group at the 5-position accelerated the activity, as the (4'-OCH<sub>3</sub>, 5-OCH<sub>3</sub>) derivative <b>41</b> and (4'-OCH<sub>3</sub>, 5-CH<sub>3</sub>) derivative <b>45</b> were, respectively, 4-5 times more potent than the 4'-OCH<sub>3</sub> derivative (<i>S</i>)-<b>5</b>.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 4","pages":"262-270"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}