Journal of Pesticide Science最新文献

Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves 吡蚜酮对小麦叶片上禾谷蓝霉菌感染过程和细胞学特征的影响

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-08-27 DOI: 10.1584/jpestics.d24-005

Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani

{"title":"Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves","authors":"Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani","doi":"10.1584/jpestics.d24-005","DOIUrl":"https://doi.org/10.1584/jpestics.d24-005","url":null,"abstract":"Pyriofenone demonstrates outstanding efficacy in controlling powdery mildew. We investigated the impact of pyriofenone on the infection processes and cytological features of Blumeria graminis f. sp. tritici on wheat leaves. The preventive application of pyriofenone before inoculation did not inhibit conidial germination but effectively suppressed both appressorial and haustorial formation. Notably, haustorial formation was effectively inhibited, resulting in the complete suppression of successive lesion development and sporulation. Curative application of pyriofenone after inoculation also inhibited lesion expansion and sporulation. Furthermore, it had considerable impact on the morphogenesis of powdery mildew fungus. We observed multi-formed secondary appressoria, shrunken or bifurcated hyphae, abnormal conidiophores, and clubbed conidia-like structures. Subsequently, we employed a histochemical approach to analyze the localization of essential components for the polar growth of fungal hyphae. Pyriofenone induced mislocalization of the actin cytoskeleton, β-glucan and cytoplasmic vesicles, although it did not affect tubulin orientation.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-005/figure/advpub_D24-005.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"8 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates. 合成灼烧烯 A 衍生物并对其进行生物学评价，从而发现新型候选杀真菌剂。

IF 1.5 4区农林科学

Journal of Pesticide Science Pub Date : 2024-08-20 DOI: 10.1584/jpestics.D24-014

Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Masako Honsho, Sota Negami, Sota Honma, Katsuyuki Sakai, Toshiyuki Tokiwa, Hiroki Kojima, Kenichi Nonaka, Shin-Ichi Fuji, Takumi Chinen, Takeo Usui, Yukihiro Asami

{"title":"Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates.","authors":"Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Masako Honsho, Sota Negami, Sota Honma, Katsuyuki Sakai, Toshiyuki Tokiwa, Hiroki Kojima, Kenichi Nonaka, Shin-Ichi Fuji, Takumi Chinen, Takeo Usui, Yukihiro Asami","doi":"10.1584/jpestics.D24-014","DOIUrl":"https://doi.org/10.1584/jpestics.D24-014","url":null,"abstract":"An antifungal polyene-decalin polyketide natural product, burnettiene A (1) has been re-discovered from the culture broth of Lecanicillium primulinum (current name: Flavocillium primulinum) FKI-6715 strain utilizing our original multidrug-sensitive yeast system. This polyene-decalin polyketide natural product was originally isolated from Aspergillus burnettii. The antifungal activity of 1 against Candida albicans has been reported. However, only one fungal species for the antifungal activity of 1 has been revealed, and details of the antifungal activity against other pathogenic fungus remain unknown. After extensive screening for antifungal activity, we found that 1 exhibits broad antifungal activity against pathogenic plant fungi, including Colletotrichum gloeosporioides, Botrytis cinerea, Pyricularia oryzae, Leptosphaeria maculans, and Rhizoctonia solani. Furthermore, we synthesized 12 derivatives from 1 and evaluated their antifungal activity to reveal the detailed structure-activity relationship. The methyl ester derivative showed antifungal activity against Saccharomyces cerevisiae 12geneΔ0HSR-iERG6 100-fold more potent than that of 1. Our research indicates that 1 would be a promising natural product as a new fungicidal candidate and the methyl ester derivative especially has great potential.","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 3","pages":"159-167"},"PeriodicalIF":1.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana 拟南芥通过绞股蓝内酯介导的免疫启动增强对灰霉病的抗病性

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-08-09 DOI: 10.1584/jpestics.d24-019

Moeka Fujita, Tomoya Tanaka, Miyuki Kusajima, Kengo Inoshima, Futo Narita, Hidemitsu Nakamura, Tadao Asami, Akiko Maruyama-Nakashita, Hideo Nakashita

{"title":"Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana","authors":"Moeka Fujita, Tomoya Tanaka, Miyuki Kusajima, Kengo Inoshima, Futo Narita, Hidemitsu Nakamura, Tadao Asami, Akiko Maruyama-Nakashita, Hideo Nakashita","doi":"10.1584/jpestics.d24-019","DOIUrl":"https://doi.org/10.1584/jpestics.d24-019","url":null,"abstract":"Strigolactones (SLs) are a class of plant hormones that play several roles in plants, such as suppressing shoot branching and promoting arbuscular mycorrhizal symbiosis. The positive regulation of plant disease resistance by SLs has recently been demonstrated by analyses using SL-related mutants. In Arabidopsis, SL-mediated signaling has been reported to modulate salicylic acid-mediated disease resistance, in which the priming of plant immunity plays an important role. In this study, we analyzed the effect of the synthetic SL analogue rac-GR24 on resistance against necrotrophic pathogen Botrytis cinerea. In rac-GR24-treated plants, disease resistance against B. cinerea was enhanced in an ethylene- and camalexin-dependent manners. Expression of the ethylene-related genes and the camalexin biosynthetic gene and camalexin accumulation after pathogen infection were enhanced by immune priming in rac-GR24-treated plants. These suggest that SL-mediated immune priming is effective for many types of resistance mechanisms in plant self-defense systems.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-019/figure/advpub_D24-019.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"107 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry 使用日本官方分析方法 QuEChERS 和改良 QuEChERS 结合同位素稀释质谱法，可靠定量糙米样品中的有机磷农药，以进行能力验证

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-07-24 DOI: 10.1584/jpestics.d24-021

Takamitsu Otake, Keisuke Nakamura, Naoyuki Hirabayashi, Takaho Watanabe

{"title":"A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry","authors":"Takamitsu Otake, Keisuke Nakamura, Naoyuki Hirabayashi, Takaho Watanabe","doi":"10.1584/jpestics.d24-021","DOIUrl":"https://doi.org/10.1584/jpestics.d24-021","url":null,"abstract":"The objective of the present study is to provide reliable concentration values as assigned values for target pesticides in brown rice samples used in proficiency testing (PT) organized by the Hatano Research Institute (HRI). The test samples for PT were prepared by immersing brown rice in the pesticide solution and using a spray dryer by the HRI. Homogeneity and stability assessments were performed for PT samples, and the relative uncertainties due to inhomogeneity and instability were 0.58 %–0.78 % and 0 %–0.96 %, respectively. Quantification for the assigned values of target pesticides by the National Metrology Institute of Japan (NMIJ) was carried out using the multiple analytical methods including Japanese official analytical method, QuEChERS, and modified QuEChERS, which were combined with isotope dilution mass spectrometry, to ensure the reliability of the analytical results. The NMIJ assigned values were 0.065±0.004 mg/kg for chlorpyrifos, 0.217±0.012 mg/kg for diazinon, 0.138±0.008 mg/kg for fenitrothion, and 0.138±0.008 mg/kg for malathion.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-021/figure/advpub_D24-021.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"95 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS 细菌杀虫剂：作用机理、食品污染的可能性以及使用 MS 进行残留分析

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-07-19 DOI: 10.1584/jpestics.d24-006

Hiroto Tamura

引用次数: 0

High-throughput screening for novel Bacillus thuringiensis insecticidal proteins revealed evidence that the bacterium exchanges Domain III to enhance its insecticidal activity 高通量筛选新型苏云金芽孢杆菌杀虫蛋白的结果表明，苏云金芽孢杆菌通过交换领域 III 来增强其杀虫活性

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-07-10 DOI: 10.1584/jpestics.d24-011

Ruth Cong, Jing-Tong Hou, Takashi Yamamoto

{"title":"High-throughput screening for novel Bacillus thuringiensis insecticidal proteins revealed evidence that the bacterium exchanges Domain III to enhance its insecticidal activity","authors":"Ruth Cong, Jing-Tong Hou, Takashi Yamamoto","doi":"10.1584/jpestics.d24-011","DOIUrl":"https://doi.org/10.1584/jpestics.d24-011","url":null,"abstract":"Approximately 3000 Bacillus thuringiensis (Bt) isolates were screened to discover novel three-domain (3D) Cry proteins active against Helicoverpa zea (corn earworm). From 400 active isolates found during the primary screening, Cry1Ac and Cry2A, which are known to be active against H. zea, were removed using multiplex-primer PCR and high-throughput column chromatography. This process reduced the number of active cultures to 48. DNA segments encoding Domain III of these 48 cultures were amplified by PCR and sequenced. Sequencing revealed two novel Cry1B-type Domain IIIs. Further sequencing of the flanking regions of these domains revealed that one was part of Cry1Bj (GenBank: KT952325). However, the other Domain III lacked Domains I and II. Instead, this Domain III was associated with two open reading frames, ORF1 and ORF2. ORF1 was identified as an ATP-binding protein, and ORF2 as an ATPase, suggesting that Bt exchanges Domain III among homologous Cry proteins.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-011/figure/advpub_D24-011.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"5 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141584872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoencapsulated deltamethrin combined with indoxacarb: An effective synergistic association against aphids 纳米胶囊化溴氰菊酯与茚虫威的结合：防治蚜虫的有效协同作用

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-07-09 DOI: 10.1584/jpestics.d24-003

Marine Galloux, Guillaume Bastiat, Corinne Lefrancois, Véronique Apaire-Marchais, Caroline Deshayes

{"title":"Nanoencapsulated deltamethrin combined with indoxacarb: An effective synergistic association against aphids","authors":"Marine Galloux, Guillaume Bastiat, Corinne Lefrancois, Véronique Apaire-Marchais, Caroline Deshayes","doi":"10.1584/jpestics.d24-003","DOIUrl":"https://doi.org/10.1584/jpestics.d24-003","url":null,"abstract":"Widespread pesticide use for decades has caused environmental damage, biodiversity loss, serious human and animal health problems, and resistance to insecticides. Innovative strategies are needed to reduce treatment doses in pest management and to overcome insecticide resistance. In the present study, combinations of indoxacarb, an oxadiazine insecticide, with sublethal concentrations of deltamethrin encapsulated in lipid nanocapsules, have been tested on the crop pest Acyrthosiphon pisum. In vivo toxicological tests on A. pisum larvae have shown a synergistic effect of nanoencapsulated deltamethrin with a low dose of indoxacarb. Furthermore, the stability of deltamethrin nanoparticles has been demonstrated in vitro under different mimicking environmental conditions. In parallel, the integrity and stability of lipid nanoparticles in the digestive system of aphid larvae over time have been observed by Förster Resonance Energy Transfer (FRET) imaging. Thus, the deltamethrin nanocapsules/indoxacarb synergistic association is promising for the development of future formulations against pest insects to reduce insecticide doses.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-003/figure/advpub_D24-003.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"59 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141575818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advantageous properties of a new fungicide, isofetamid 新型杀菌剂异氟菌酰胺的优势特性

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-05-30 DOI: 10.1584/jpestics.d23-067

Shuko Nishimi, Yuzuka Abe, Nanami Kuwahara, Akihiro Nishimura, Shintaro Tsukuda, Satoshi Araki, Kosuke Tsunematsu, Yohei Fukumori, Munekazu Ogawa, Kazumi Suzuki, Shigeru Mitani

{"title":"Advantageous properties of a new fungicide, isofetamid","authors":"Shuko Nishimi, Yuzuka Abe, Nanami Kuwahara, Akihiro Nishimura, Shintaro Tsukuda, Satoshi Araki, Kosuke Tsunematsu, Yohei Fukumori, Munekazu Ogawa, Kazumi Suzuki, Shigeru Mitani","doi":"10.1584/jpestics.d23-067","DOIUrl":"https://doi.org/10.1584/jpestics.d23-067","url":null,"abstract":"The fungicidal properties of a new fungicide, isofetamid, were examined to assess its antifungal spectrum, mode of action, and effects on the infection process of Botrytis cinerea. Additionally, we investigated its fungicidal activity against isolates of B. cinerea resistant to existing fungicides. In mycelial growth inhibition tests, isofetamid exhibited excellent fungicidal activity against ascomycetes but showed no activity against basidiomycetes and oomycetes. Respiratory enzyme assay using mitochondria revealed that isofetamid inhibited succinate dehydrogenase activity prepared from B. cinerea and other ascomycetes fungi used in the study. On the other hand, the activity of mitochondria prepared from Pythium, potato and rat were not inhibited. Isofetamid inhibited also many stages of the infection processes in B. cinerea. Furthermore, it exhibited high fungicidal activity against B. cinerea isolates that were resistant to existing fungicides.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D23-067/figure/advpub_D23-067.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"19 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141196171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics analysis of the effects of chelerythrine on Ustilaginoidea virens. 代谢组学分析切瑞瑟林对乌斯提拉吉诺虫（Ustilaginoidea virens）的影响

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-05-20 DOI: 10.1584/jpestics.D23-065

Qinghui Wei, Xihai Zhai, Weifeng Song, Zhiyong Li, Yaqing Pan, Baoying Li, Zhanli Jiao, Zhenghao Shi, Jiangtao Yu

{"title":"Metabolomics analysis of the effects of chelerythrine on Ustilaginoidea virens.","authors":"Qinghui Wei, Xihai Zhai, Weifeng Song, Zhiyong Li, Yaqing Pan, Baoying Li, Zhanli Jiao, Zhenghao Shi, Jiangtao Yu","doi":"10.1584/jpestics.D23-065","DOIUrl":"10.1584/jpestics.D23-065","url":null,"abstract":"Rice false smut (RFS) caused by Ustilaginoidea virens is widely distributed in major rice-producing regions. Previous studies have shown that treating RFS with chelerythrine can decrease the germination of fungus spores by 86.7% and induce fungal cell apoptosis. In the present study, the effects of chelerythrine on the metabolism of U. virens explored using metabolomics and analyses of differentially accumulated metabolites and altered metabolic pathways. The top 15 metabolites in random forest analysis were significantly different between groups. In positive ion mode, purine, phenylalanine metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, pyrimidine metabolism, and nitrogen metabolism were dominant. Alanine, aspartate, glutamate metabolism, and phenylalanine metabolism were enriched in negative ion mode. Differentially expressed genes and altered metabolic pathways of U. virens were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine and provide a theoretical basis for targeted drug delivery.","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 2","pages":"104-113"},"PeriodicalIF":2.4,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci 田间调查田蓟马的繁殖模式和与除虫菊酯抗性有关的钠通道突变

IF 2.4 4区农林科学

Journal of Pesticide Science Pub Date : 2024-05-18 DOI: 10.1584/jpestics.d24-009

Yui Tomizawa, Misato Aizawa, Akiya Jouraku, Shoji Sonoda

{"title":"Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci","authors":"Yui Tomizawa, Misato Aizawa, Akiya Jouraku, Shoji Sonoda","doi":"10.1584/jpestics.d24-009","DOIUrl":"https://doi.org/10.1584/jpestics.d24-009","url":null,"abstract":"Using PCR-Restriction Fragment Length Polymorphism (RFLP) with mitochondrial cytochrome c oxidase subunit I sequences, we examined the reproductive modes of female adults of Thrips tabaci collected at 54 sites across Japan. Results showed the presence of heteroplasmic insects harboring mitochondria associated with arrhenotoky and thelytoky. Using the insects, we also applied PCR-RFLP to examine the genotypes for the amino acid mutation (T929I) site involved in pyrethroid resistance. Findings showed the presence of thelytokous heterozygotes under the circumstance that most arrhenotokous insects are resistant homozygotes, and many thelytokous insects are susceptible homozygotes. These results suggest that, in the field, genetic exchange occurs between insects through of both reproductive modes. A survey of the genotypes for the other amino acid mutations using nucleotide sequencing showed a decline of insects with an M918T and L1014F pair and an increase of insects with M918L. These results suggest the evolutional progression of amino acid mutations associated with pyrethroid resistance in T. tabaci.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-009/figure/advpub_D24-009.png\"/>\u0000Fullsize Image","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"53 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0