Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci

IF 1.5 4区农林科学 Q2 ENTOMOLOGY

Journal of Pesticide Science Pub Date : 2024-05-18 DOI:10.1584/jpestics.d24-009

Yui Tomizawa, Misato Aizawa, Akiya Jouraku, Shoji Sonoda

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Abstract

Using PCR-Restriction Fragment Length Polymorphism (RFLP) with mitochondrial cytochrome c oxidase subunit I sequences, we examined the reproductive modes of female adults of Thrips tabaci collected at 54 sites across Japan. Results showed the presence of heteroplasmic insects harboring mitochondria associated with arrhenotoky and thelytoky. Using the insects, we also applied PCR-RFLP to examine the genotypes for the amino acid mutation (T929I) site involved in pyrethroid resistance. Findings showed the presence of thelytokous heterozygotes under the circumstance that most arrhenotokous insects are resistant homozygotes, and many thelytokous insects are susceptible homozygotes. These results suggest that, in the field, genetic exchange occurs between insects through of both reproductive modes. A survey of the genotypes for the other amino acid mutations using nucleotide sequencing showed a decline of insects with an M918T and L1014F pair and an increase of insects with M918L. These results suggest the evolutional progression of amino acid mutations associated with pyrethroid resistance in T. tabaci.

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田间调查田蓟马的繁殖模式和与除虫菊酯抗性有关的钠通道突变

利用线粒体细胞色素 c 氧化酶亚单位 I 序列的 PCR-限制性片段长度多态性（RFLP），我们研究了在日本 54 个地点采集的蓟马雌成虫的繁殖模式。结果表明，存在线粒体与 arrhenotoky 和 thelytoky 相关的异质昆虫。利用这些昆虫，我们还采用 PCR-RFLP 技术检测了涉及拟除虫菊酯抗性的氨基酸突变（T929I）位点的基因型。结果表明，在大多数 arhenotokous 昆虫是抗性同源杂合子，而许多 thelytokous 昆虫是易感同源杂合子的情况下，存在 thelytokous 杂合子。这些结果表明，在田间，昆虫之间会通过这两种繁殖模式进行基因交换。通过核苷酸测序对其他氨基酸突变基因型的调查显示，带有 M918T 和 L1014F 基因对的昆虫数量减少，而带有 M918L 基因对的昆虫数量增加。这些结果表明，与 T. tabaci 的拟除虫菊酯抗性相关的氨基酸突变在不断进化。

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来源期刊

Journal of Pesticide Science 农林科学-昆虫学

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

18-36 weeks

期刊介绍： The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.