{"title":"代谢组学分析切瑞瑟林对乌斯提拉吉诺虫(Ustilaginoidea virens)的影响","authors":"Qinghui Wei, Xihai Zhai, Weifeng Song, Zhiyong Li, Yaqing Pan, Baoying Li, Zhanli Jiao, Zhenghao Shi, Jiangtao Yu","doi":"10.1584/jpestics.D23-065","DOIUrl":null,"url":null,"abstract":"<p><p>Rice false smut (RFS) caused by <i>Ustilaginoide</i>a virens is widely distributed in major rice-producing regions. Previous studies have shown that treating RFS with chelerythrine can decrease the germination of fungus spores by 86.7% and induce fungal cell apoptosis. In the present study, the effects of chelerythrine on the metabolism of <i>U. virens</i> explored using metabolomics and analyses of differentially accumulated metabolites and altered metabolic pathways. The top 15 metabolites in random forest analysis were significantly different between groups. In positive ion mode, purine, phenylalanine metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, pyrimidine metabolism, and nitrogen metabolism were dominant. Alanine, aspartate, glutamate metabolism, and phenylalanine metabolism were enriched in negative ion mode. Differentially expressed genes and altered metabolic pathways of <i>U. virens</i> were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine and provide a theoretical basis for targeted drug delivery.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"49 2","pages":"104-113"},"PeriodicalIF":1.5000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176050/pdf/","citationCount":"0","resultStr":"{\"title\":\"Metabolomics analysis of the effects of chelerythrine on <i>Ustilaginoidea virens</i>.\",\"authors\":\"Qinghui Wei, Xihai Zhai, Weifeng Song, Zhiyong Li, Yaqing Pan, Baoying Li, Zhanli Jiao, Zhenghao Shi, Jiangtao Yu\",\"doi\":\"10.1584/jpestics.D23-065\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rice false smut (RFS) caused by <i>Ustilaginoide</i>a virens is widely distributed in major rice-producing regions. Previous studies have shown that treating RFS with chelerythrine can decrease the germination of fungus spores by 86.7% and induce fungal cell apoptosis. In the present study, the effects of chelerythrine on the metabolism of <i>U. virens</i> explored using metabolomics and analyses of differentially accumulated metabolites and altered metabolic pathways. The top 15 metabolites in random forest analysis were significantly different between groups. In positive ion mode, purine, phenylalanine metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, pyrimidine metabolism, and nitrogen metabolism were dominant. Alanine, aspartate, glutamate metabolism, and phenylalanine metabolism were enriched in negative ion mode. Differentially expressed genes and altered metabolic pathways of <i>U. virens</i> were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine and provide a theoretical basis for targeted drug delivery.</p>\",\"PeriodicalId\":16712,\"journal\":{\"name\":\"Journal of Pesticide Science\",\"volume\":\"49 2\",\"pages\":\"104-113\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176050/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pesticide Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1584/jpestics.D23-065\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.D23-065","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
由 Ustilaginoidea virens 引起的水稻烟粉虱(RFS)广泛分布于水稻主产区。以往的研究表明,用白屈菜红碱处理水稻假烟病可使真菌孢子的萌发率降低 86.7%,并诱导真菌细胞凋亡。本研究采用代谢组学方法,分析了不同积累的代谢物和改变的代谢途径,探讨了白屈菜对紫云英新陈代谢的影响。在随机森林分析中,前 15 种代谢物在组间存在显著差异。在正离子模式下,嘌呤、苯丙氨酸代谢、苯丙氨酸、酪氨酸、色氨酸生物合成、嘧啶代谢和氮代谢占主导地位。丙氨酸、天门冬氨酸、谷氨酸代谢和苯丙氨酸代谢以负离子模式富集。不同表达的基因和改变的代谢途径对 U. virens 产生了影响。这些研究结果支持了未来利用白屈菜红碱预防和治疗RFS的研究,并为靶向给药提供了理论依据。
Metabolomics analysis of the effects of chelerythrine on Ustilaginoidea virens.
Rice false smut (RFS) caused by Ustilaginoidea virens is widely distributed in major rice-producing regions. Previous studies have shown that treating RFS with chelerythrine can decrease the germination of fungus spores by 86.7% and induce fungal cell apoptosis. In the present study, the effects of chelerythrine on the metabolism of U. virens explored using metabolomics and analyses of differentially accumulated metabolites and altered metabolic pathways. The top 15 metabolites in random forest analysis were significantly different between groups. In positive ion mode, purine, phenylalanine metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, pyrimidine metabolism, and nitrogen metabolism were dominant. Alanine, aspartate, glutamate metabolism, and phenylalanine metabolism were enriched in negative ion mode. Differentially expressed genes and altered metabolic pathways of U. virens were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine and provide a theoretical basis for targeted drug delivery.
期刊介绍:
The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.