Journal of nutritional science and vitaminology最新文献

{"title":"A Compound Nutritional Supplement Relieving the Core Symptoms of Autism Rats.","authors":"Xinrong Pan, Yaoyao Xiu, Shiting Huang, Jinjie Lai, Xiaoxue Wang, Xinhui Cao, Liqun Jiang","doi":"10.3177/jnsv.71.34","DOIUrl":"10.3177/jnsv.71.34","url":null,"abstract":"<p><p>Autism, with a global morbidity of approximately 0.6% annually, significantly burdens on families and society. A predominant characteristic among autistic children is intestinal dysfunction, generating nutrient absorption issues. This often causes vitamin and mineral deficiencies, which significantly impacts neurological development. To address this, we have developed a comprehensive nutritional supplement containing compound vitamins, minerals, and gut bacteria. The aim of this study is to investigate the behavioral effects of this supplement on autism rats. The supplement formulated in this study encompasses compound vitamins, minerals, and intestinal bacteria. The autism offspring model (VPA model) was established through intraperitoneal injection of sodium valproate into pregnant rats. Behavioral assessments, gastrointestinal microbiota analysis, and brain development evaluations were conducted to assess the effects of the supplement on VPA offspring rats. A nutritional supplement enriched with vitamins (VB6, VB12, VC, VD, folic acid), minerals (calcium, magnesium), and bifidobacteria was prepared. Open field experiments demonstrated a 3-mo supplementation intervention effectively alleviated anxiety symptoms and enhanced curiosity levels in VPA offspring. The bead embedding experiment revealed the supplementation significantly improved stereotypical behaviors in VPA offspring. The nesting experiment showed a 3-mo supplementation intervention effectively enhanced cognitive abilities in VPA offspring rats. The three-box social experiment demonstrated the supplementation improved social novelty and tendency in VPA offspring. Analysis of gastrointestinal microbiota revealed the supplementation modulated the gut microbiome, promoting better nutrient absorption and overall health. Our findings suggest the comprehensive nutritional supplement, containing compound vitamins, minerals, and gut bacteria, effectively alleviates core symptoms of autism in rats. This study provides valuable insights into the potency of nutritional interventions in improving the quality of life for autistic individuals. Future research is warranted to further explore the mechanisms underlying these observed benefits and to assess the long-term effects of this supplement in larger clinical trials.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 1","pages":"34-45"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Excessive Sucrose Intake on Aggressive Behavior and Peripheral Stress-Related Hormone and Catecholamines in BALB/c Mice during Adolescent Development.","authors":"Tohru Miyata, Eichi Nojima, Yuji Minai","doi":"10.3177/jnsv.71.16","DOIUrl":"10.3177/jnsv.71.16","url":null,"abstract":"<p><p>As binge eating and unbalanced diets increase the prevalence of obesity and metabolic disorders, it has been noted that the increase in psychiatric disorders is also a consequence of diet. The present study examined aggressive behavior and peripheral stress-related hormone and catecholamine levels in BALB/c mice fed a high-sucrose diet during adolescent development. BALB/c mice are rarely used in research assessing the effect of diet, but were used for ethnic and personal differences and as a new experimental model. BALB/c mice were fed a diet in which all carbohydrate components were replaced with sucrose for 4 wk and were subjected to the resident-intruder and social dominance tube test. Plasma insulin, corticosterone, and catecholamine levels were also compared to mice fed a control diet. The high-sucrose diet did not alter body weight, glucose tolerance, and plasma insulin levels in BALB/c mice, indicating that the diet was resilient to obesity. Mice fed a high-sucrose diet exhibited increased aggressive behaviors in the resident-intruder test and had a significantly higher win rate in the tube test. Increases in adrenal weight and plasma corticosterone as well as noradrenaline and adrenaline levels were exhibited in mice fed a high-sucrose diet. In particular, this is the first evidence of increased social dominance and hyperplasia of the adrenal glands by a sucrose diet. Sucrose diet intake increased aggression in mice and caused elevated peripheral hormones involving the HPA axis and the sympathetic nervous system, indicating that this may be a central nervous system-mediated effect of excess sucrose.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 1","pages":"16-24"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of α- and δ-Tocotrienol on Human Airway Smooth Muscle Cells~Tocotrienols as Potential Candidates for the Treatment of Airway Remodeling in Asthma.","authors":"Ryota Okazaki, Aditya Sri Listyoko, Tomoya Harada, Miki Takata, Masato Morita, Hiroki Ishikawa, Yoshihiro Funaki, Genki Inui, Akira Yamasaki","doi":"10.3177/jnsv.71.300","DOIUrl":"https://doi.org/10.3177/jnsv.71.300","url":null,"abstract":"<p><p>Tocotrienols are members of the vitamin E family and exhibit antioxidant properties, immunomodulatory effects, and anti-inflammatory actions. Previously, we demonstrated that γ-tocotrienol inhibits human airway smooth muscle (ASM) cell proliferation, migration, contractile phenotype expression, and extracellular matrix protein synthesis by suppressing RhoA activation. In this study, we investigated whether α- or δ-tocotrienol modulates transforming growth factor-beta 1 (TGF-β1)-induced contractile phenotype expression in human ASM cells and platelet-derived growth factor-BB (PDGF-BB)-induced proliferation and migration of ASM cells. Human ASM cells were pretreated with α- or δ-tocotrienol before stimulation with PDGF-BB to promote proliferation and migration or with TGF-β1 to induce smooth muscle actin expression. PDGF-BB-stimulated ASM cell proliferation and migration were assessed using colorimetric and transwell migration assays. Additionally, we examined the signaling pathways involved in the effects of α- or δ-tocotrienol on PDGF-BB-induced ASM proliferation and migration, as well as TGF-β1-induced smooth muscle actin expression. TGF-β1 increased α-smooth muscle actin expression in human ASM cells. Treatment with α- and δ-tocotrienol slightly reduced α-smooth muscle actin levels, though this reduction was not statistically significant. In contrast, PDGF-BB-induced ASM cell proliferation and migration were significantly inhibited by α- and δ-tocotrienol treatment. The effects of α- and δ-tocotrienol on ASM proliferation and migration involve the RhoA signaling pathway and a reduction in reactive oxygen species (ROS) production. These findings suggest that α- and δ-tocotrienol exert beneficial effects on airway remodeling in asthma by inhibiting the proliferation and migration of human ASM cells.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 4","pages":"300-311"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D Deficiency Is Associated with an Increased Risk of Macroalbuminuria in Chinese Patients with Type 2 Diabetes.","authors":"Xiulin Wang, Wenjin Xiao","doi":"10.3177/jnsv.71.283","DOIUrl":"https://doi.org/10.3177/jnsv.71.283","url":null,"abstract":"<p><p>This research examines the correlation between serum 25-hydroxyvitamin D [25(OH)D] levels and albuminuria. A total of 203 hospitalized patients diagnosed with type 2 diabetes mellitus (T2DM) were selected from February to October 2023 and categorized into groups according to their urine albumin-to-creatinine ratio (UACR). Spearman correlation analysis and multiple regression analysis were used to assess the relationship between 25(OH)D and UACR. Among the 203 T2DM patients included, the prevalence of vitamin D deficiency was 59.1%. The 25(OH)D levels in the macroalbuminuria group 9.37 ng/mL (5.98, 15.60) were significantly lower than those in the normal albuminuria group 18.26 ng/mL (14.40, 23.52) and microalbuminuria group 18.20 ng/mL (11.71, 24.20) with statistical significance (p<0.001). Spearman correlation analysis showed a negative correlation between serum 25(OH)D and UACR (r=-0.173, p=0.014). Stepwise linear regression analysis, after adjusting for confounding factors, revealed a linear negative correlation between 25(OH)D and albuminuria (β=-0.278, p<0.001). In the multivariable logistic regression analysis, no association was identified between vitamin D deficiency and microalbuminuria in patients with T2DM. However, vitamin D deficiency may significantly increase the risk of macroalbuminuria in patients with T2DM,with an odds ratio (OR) of 4.747 (95% CI: 1.157-19.473). Vitamin D deficiency is prevalent among the study population. Serum 25(OH)D levels exhibited a significant negative correlation with UACR, suggesting a relationship between vitamin D deficiency and an elevated risk of macroalbuminuria in individuals with T2DM.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 4","pages":"283-289"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adult Chicken Bone-Derived Components Reverse the Impaired Calcium Homeostasis and Bone Mass in Mice Lacking 1,25(OH)₂D₃-VDR Signaling.","authors":"Tamao Nishiura, Hitoki Yamanaka, Risako Mori, Shigeaki Kato, Masato Nakane, Satoshi Kotoura, Ritsuko Masuyama","doi":"10.3177/jnsv.71.81","DOIUrl":"10.3177/jnsv.71.81","url":null,"abstract":"<p><p>Female adult chickens, known as laying hens, possess a distinctive bone structure in the intracortical region, which is responsible for storing calcium. Given the cyclical nature of calcium storage and demand during the egg-laying cycle, the medullary bone of laying hens plays a crucial role in maintaining calcium homeostasis. In this study, we examined the potential of orally administered components derived from adult chicken bones to improve calcium homeostasis and bone mass in mice. Samples from adult chickens and young chickens without egg-laying experience were prepared by pressing meat parts, including bones, and administered to wild type (WT) and vitamin D receptor knockout (VDRKO) mice respectively. The phenotypes observed in VDRKO mice, such as severe reductions in serum calcium concentration and bone mass, were normalized in mice fed the adult chicken bone-containing diet to the same extent as in WT mice. These effects were not observed in mice fed a young chicken bone-containing diet. The adult chicken bone-containing diet increased apparent calcium absorption in VDRKO mice compared to other dietary groups. To determine the effects on bone metabolism, osteoclasts activity was evaluated by histological measurements and the quantification of serum osteoclast marker, and it was restored by the adult chicken bone-containing diet. In addition, the treatment of adult chicken bone-derived components increased osteoclasts differentiation in vitro from cultured bone marrow macrophage. These results show that adult chicken bone-derived components improve calcium and bone homeostasis in mice lacking vitamin D action through combined effects that target calcium metabolism and bone turnover.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 1","pages":"81-92"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Blue Rose Extract Powder Intake on Skin Characteristics in Middle-Aged and Older Japanese Women-Randomized, Double-Blind, and Placebo-Controlled Trial.","authors":"Daisuke Aoyagi, Tomoe Yamada-Kato, Isao Okunishi","doi":"10.3177/jnsv.71.163","DOIUrl":"https://doi.org/10.3177/jnsv.71.163","url":null,"abstract":"<p><p>In this study, we aimed at evaluating the effect of improving skin conditions on petal-derived blue rose extract (BRE) powder intake in middle-aged and older women in Japan. We conducted a randomized, double-blind, and placebo-controlled parallel study in 48 healthy Japanese women aged 40-50 y who were aware of dry skin. We divided the participants equally into two groups (i.e., 24-24 in the test and control groups, respectively). The participants consumed 100 mg either the placebo or BRE powder daily for 4 wk. We performed skin measurements before-and-after 4 wk of continuous intake. Upon 4 wk after continuous intake, the BRE group displayed improved skin quality compared with the control group. The primary outcome was stratum corneum water content, which significantly improved in the BRE group. The secondary outcomes, melanin index, stains, wrinkles, and rough texture showed improvements between the groups as well. Visual perception, roughness of texture, and wrinkles were improved between the groups. Finally, transparency yielded better scores within the groups. This study presents the results of the first functional test targeting BRE, unraveling various effect of improving skin condition and highlighting the potential of taking BRE in skin care.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 2","pages":"163-172"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified Irwin Test with Single Intravenous Administration of Caffeine in Mice.","authors":"Sung-Hyun Cho, Su-Jeong Kim, Caglar Doguer, Miae Doo, Jung-Heun Ha","doi":"10.3177/jnsv.71.267","DOIUrl":"https://doi.org/10.3177/jnsv.71.267","url":null,"abstract":"<p><p>This study investigated the behavioral effects of intravenous caffeine administration in mice using a modified Irwin test. Thirty-two male ICR mice were divided into four groups and received intravenous doses of caffeine: 0 (control), 5, 10, or 20 mg/kg. Behavioral observations were conducted at 0, 0.25, 1.5, 6, and 24 h post administration. Notable traction, locomotion, and respiratory rate changes were observed at 10 and 20 mg/kg doses. Specifically, traction was altered at 0.25 and 1.5 h in the 10 mg/kg group, with more frequent alterations in the 20 mg/kg group. Increased locomotion (grade 6, characterized by frequent grooming and rapid movement) was observed in all the mice at 10 and 20 mg/kg doses between 0.25 and 1.5 h. Respiratory rate changes were observed only in the 20 mg/kg group during the same period. Our findings indicate that intravenous caffeine exposure significantly affects traction and locomotion, providing valuable insights for safe pharmacological testing of the central nervous system.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 3","pages":"267-271"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Screen Time and Lifestyle Habits among Upper Elementary School Children.","authors":"Asami Baba, Rikako Yoshitake, Yumiko Inose, Naomi Omi","doi":"10.3177/jnsv.71.248","DOIUrl":"https://doi.org/10.3177/jnsv.71.248","url":null,"abstract":"<p><p>This cross-sectional study examined the link between screen time and lifestyle habits in upper elementary school children. It included 400 elementary schoolchildren studying in the fifth and sixth grades in elementary (187 boys, 213 girls, age: 10-12 y) in Japan. Gender, obesity index, physical fitness score, screen time, and lifestyle habits related to breakfast, midnight snacks, exercise, and sleep were the study items. Using logistic regression analysis, we examined lifestyle habits factors and screen time as the dependent and independent variables, respectively. We found that >3 h of screen time was significantly associated with increased odds of eating breakfast sometimes or never (odds ratio [95% confidence interval] 2.37 [1.05, 5.38]) and eating midnight snacks (2.72 [1.41, 5.23]). Additionally, the same factor significantly associated with <6 h of sleep (10.45 [2.78, 39.30]) and bedtime later than 10 pm (2.81 [1.43, 5.53]) and 11 pm (3.97 [1.95, 8.07]). These results hold true even after accounting for demographics, obesity index, and physical fitness score. Conversely, 2-3 h of screen time was significantly associated with sleep onset conditions (2.05 [1.17, 3.58]), whereas >3 h of screen time was not. Our results showed that increased screen time was associated with skipping breakfast, midnight snacks, sleep onset conditions, and less bedtime.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 3","pages":"248-255"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum 25-Hydroxyvitamin D Levels and the Risk of Post-Traumatic Wound Infection.","authors":"Zhanyi Zhang, Xizhe Yang, Mengyuan Li, Bo Li","doi":"10.3177/jnsv.71.110","DOIUrl":"https://doi.org/10.3177/jnsv.71.110","url":null,"abstract":"<p><p>Post-traumatic wound infection is commonly observed in trauma patients and has a poor prognosis. Vitamin D is a significant modulator of the immune system and may play a role in preventing wound infection. However, the inherent limitations and inconsistent results of previous observational studies restricted them from inferring causal effects. We employed Mendelian randomization design to estimate the causal effects of serum 25-hydroxyvitamin D levels on the risk of post-traumatic wound infection. Data in the analysis were extracted from large-scale genome-wide association studies with 417,580 individuals for serum 25-hydroxyvitamin D measurements and 218,546 individuals for post-traumatic wound infection. Multivariable Mendelian randomization analyses were performed to adjust for confounding variables. We also conducted extensive sensitivity analyses to verify the main results. The results revealed a causal relationship between higher serum 25-hydroxyvitamin D levels and a lower post-traumatic wound infection risk (OR=0.473, p=0.014). The causality remained after adjustment of potential confounding factors according to Multivariable Mendelian randomization analyses (OR=0.361, p<0.001). Sensitivity tests suggested the reliability of our findings. This study indicated a causal association between genetically predicted high serum 25-hydroxyvitamin D levels and a decreased risk of post-traumatic wound infection.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 2","pages":"110-117"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thanks to Reviewers.","authors":"","doi":"10.3177/jnsv.71.184","DOIUrl":"https://doi.org/10.3177/jnsv.71.184","url":null,"abstract":"","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 2","pages":"184"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}