Journal of Ovarian Research最新文献

{"title":"The BDNF/TrkB/CREB signaling improves chronic stress-induced decreased ovarian reserve by inhibiting pyroptosis of granulosa cells and disorders of sex hormone secretion.","authors":"Yuanyuan Chen, Jun Tang, Yi Wen, Zhaohua Liu, Yi Zhang, Man Luo","doi":"10.1186/s13048-026-02111-z","DOIUrl":"10.1186/s13048-026-02111-z","url":null,"abstract":"<p><strong>Background: </strong>Decreased ovarian reserve (DOR) seriously affects female reproductive health. However, the mechanism of DOR remains unknown. This study aimed to explore the mechanism of BDNF/TrkB/CREB signaling in granulosa cell pyroptosis and sex hormone secretion in DOR.</p><p><strong>Methods: </strong>Recombinant BDNF (rBDNF) and TrkB antagonist ANA-12 were used to investigate the effects of the BDNF-TrkB signaling pathway on chronic unpredictable stress (CUS)-induced DOR in vivo. The mechanism of the BDNF-TrkB signaling pathway in NE-induced pyroptosis and sex hormone secretion was further investigated in vitro by the combined application of the CREB antagonist KG-501.</p><p><strong>Results: </strong>The CUS model reduced BDNF expression in brain tissue, peripheral blood, and ovarian tissues and inhibited p-TrkB/TrkB, p-PI3K/PI3K, and p-AKT/AKT expression. rBDNF enhanced the ovarian index and improved histological structure in CUS mice, restored the disordered estrus cycle, reduced the serum corticosterone level, and increased the pregnancy rate and embryo number. rBDNF inhibited serum FSH, LH, and NE levels and apoptosis in CUS mice while increasing AMH, E₂, P, and T levels, accompanied by downregulated NLRP3, ASC, GSDMD-N, Cleaved Caspase-1, IL-18, and IL-1β levels. However, the ANA-12 effect on the above factors was opposite to that of rBDNF. Mechanistically, the BDNF-TrkB signaling pathway inhibited NE-induced pyroptosis of granulosa cells and sex hormone secretion disorders by promoting CREB phosphorylation.</p><p><strong>Conclusion: </strong>Our findings indicate that the BDNF-TrkB signaling pathway inhibits pyroptosis of granulosa cells and sex hormone secretion disorders by promoting CREB phosphorylation, thereby improving DOR. These results provide a new theoretical basis in DOR for targeting the BDNF-TrkB-CREB axis.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review and meta-analysis of fatty acid composition in polycystic ovary syndrome: perspectives from blood and follicular fluid.","authors":"Reza Zarezadeh, Amir Fattahi, Kobra Hamdi, Mohammad Nouri, Masoud Darabi","doi":"10.1186/s13048-026-02151-5","DOIUrl":"10.1186/s13048-026-02151-5","url":null,"abstract":"<p><strong>Background & aims: </strong>A comprehensive assessment of fatty acids (FAs) in polycystic ovary syndrome (PCOS) has yet to be carried out. The current study aimed to synthesize existing evidence on the FA composition of blood and follicular fluid (FF) in women with PCOS compared to non-PCOS controls.</p><p><strong>Methods: </strong>Four electronic databases were systematically searched until February 2025 using standard keywords. Risk of bias and evidence certainty were assessed using the ROBINS-I framework and the GRADE system, respectively. Random effects models were employed to calculate pooled standardized mean differences (SMDs) with respective 95% confidence intervals (CIs). Publication bias was inspected using funnel plots, Begg's test, and Egger's test.</p><p><strong>Results: </strong>Twelve studies qualified for inclusion in the meta-analysis. Overall effect sizes for several prominent FAs, including palmitoleic acid (SMD [95% CI] = 0.389 [0.121, 0.657], p = 0.004, I² = 25.2%), oleic acid (SMD [95% CI] = 0.811 [0.107, 1.515], p = 0.024, I² = 90.0%), linoleic acid (SMD [95% CI] = 0.542 [0.349, 0.736], p < 0.001, I² = 0%), and arachidonic acid (SMD [95% CI] = 0.252 [0.053, 0.451], p = 0.013, I² = 0%), indicated an increase in the PCOS group. The direction and significance of the results partly persisted following subgroup analyses based on sample type and lipid fraction, as well as sensitivity analyses.</p><p><strong>Conclusion: </strong>The findings of this study suggest that FA profile may be altered in women with PCOS, primarily characterized by potential elevations in levels of key mono- and n-6 polyunsaturated FAs.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning-based prediction of gene expression from histopathology identifies NR5A1 as a candidate biomarker and druggable target in high-grade serous ovarian carcinoma.","authors":"Prakash Lingasamy, Marta Ostrowska-Leśko, Pantelis Tsakalis, Naisarg Patel, Ilias Chamatidis, Sajitha Lulu Sudhakaran, Joanna Kubik, Marcin Bobiński, Nikos D Lagaros, Andres Salumets, Vijayachitra Modhukur","doi":"10.1186/s13048-026-02166-y","DOIUrl":"10.1186/s13048-026-02166-y","url":null,"abstract":"<p><strong>Background: </strong>High-grade serous ovarian cancer (HGSOC) is the most lethal ovarian cancer subtype, responsible for ~ 70% of ovarian cancer-related deaths and marked by late-stage diagnosis and frequent platinum resistance. Although transcriptomic profiling enables molecular stratification and prediction of therapeutic response; routine clinical use of this approach is limited by cost and logistical constraints. Computational pathology analysis offers a scalable alternative by inferring transcriptional states directly from routine hematoxylin and eosin (H&E) whole-slide images (WSIs).</p><p><strong>Methods: </strong>Paired H&E WSIs and RNA-sequencing data from the TCGA-OV cohort, including 1,371 diagnostic H&E WSIs retrieved for preprocessing and quality control, were used to develop a self-supervised virtual-transcriptomics framework based on Momentum Contrast v2 (MoCo v2) and multi-output Random Forest regression. Model performance was assessed using patient-level five-fold cross-validation. Candidate genes were evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR) in an independent cohort of 10 HGSOC tumors, including 4 platinum responders and 6 non-responders.</p><p><strong>Results: </strong>The model predicted expression of approximately 6,400 protein-coding genes, achieving a genome-wide mean Pearson correlation of r = 0.36, with more than 300 genes showing stronger image-expression coupling (r > 0.44). RT-qPCR analysis of 18 candidate genes revealed substantial inter-patient heterogeneity. NR5A1 exhibited the highest expression variability (coefficient of variation [CV] = 1.486) and significantly higher expression in platinum-responsive tumors than in non-responders (mean 2^⁻ΔCt = 0.263 vs. 0.013; p < 0.05). Exploratory in silico docking and molecular dynamics (MD) analyses suggested structurally stable binding interactions between Steroidogenic Factor-1 (SF-1/NR5A1) and the natural plant compound cubebin.</p><p><strong>Conclusion: </strong>This study demonstrates that histological architecture contains measurable transcriptomic information that can support scalable biomarker prioritization from routine diagnostic histology in HGSOC. NR5A1 represents a hypothesis-generating candidate biomarker and structurally tractable target for future experimental studies. Future validation in larger, multi-center cohorts will be essential to confirm model robustness, biological relevance, and potential clinical utility.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148226173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term outcomes of fertility preservation counseling in women of reproductive age prior to gonadotoxic therapy.","authors":"Inha Lee, Euna Choi, Jin Kyung Baek, Hyemin Park, Heeyon Kim, Bo Hyon Yun, Jae Hoon Lee, SiHyun Cho, Young Sik Choi","doi":"10.1186/s13048-026-02160-4","DOIUrl":"10.1186/s13048-026-02160-4","url":null,"abstract":"<p><strong>Background: </strong>As survival rates for reproductive-aged women with cancer improve, the importance of fertility preservation (FP) prior to gonadotoxic chemotherapy has increased. This study evaluated long-term FP outcomes by comparing return rates and clinical pregnancy rates across FP strategies -oocyte and embryo cryopreservation and GnRH agonist use-and analyzed longitudinal ovarian reserve changes via serum anti-Müllerian hormone (AMH).</p><p><strong>Results: </strong>Among 399 women analyzed, breast cancer was the most common diagnosis (72.4%). Serum AMH declined profoundly at 6 months post-chemotherapy (3.07 to 0.08 ng/mL, p < 0.01), with partial recovery at 12 months (0.30 ng/mL), remaining significantly below baseline throughout 60-month follow-up. Longitudinal AMH data were available only for GnRH agonist-treated patients, precluding direct comparison with untreated patients. The return rate for pregnancy counseling was highest in the embryo cryopreservation group (63.4%). Total CPR among returning patients were significantly higher in the oocyte cryopreservation (70.5%) and embryo cryopreservation (69.2%) groups versus the counseling-only group (28.5%) (p = 0.020 and p = 0.014, respectively). Notably, all cryopreservation patients also received GnRH agonists; comparisons therefore reflect GnRH agonist plus cryopreservation versus GnRH agonist alone versus no active FP. GnRH agonist use alone did not show a significant advantage over counseling only.</p><p><strong>Conclusions: </strong>Chemotherapy is associated with a substantial and lasting reduction in ovarian reserve despite GnRH agonist co-treatment. Oocyte and embryo cryopreservation were associated with significantly higher CPR compared with counseling or GnRH agonist use alone, though causal inference is precluded by the retrospective design. The independent effect of GnRH agonists on ovarian reserve could not be fully assessed due to the absence of a GnRH agonist-naive control group with longitudinal follow-up. Cancer patients represent a distinct FP population with higher return rates than social FP cohorts, underscoring the importance of cancer-specific evidence for counseling. These findings support early FP counseling and suggest cryopreservation offers reproductive benefits beyond GnRH agonist use alone.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13479962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging gut microbiota and polycystic ovary syndrome: the mediating role of metabolites and immune pathways via Mendelian randomization and mediation analysis.","authors":"Yushan Li, Yan Zhang, Wei Liu, Tao Tao","doi":"10.1186/s13048-026-02162-2","DOIUrl":"10.1186/s13048-026-02162-2","url":null,"abstract":"<p><strong>Background: </strong>Polycystic Ovary Syndrome (PCOS) is a common reproductive metabolic disorder impacting women of reproductive age, with its etiology influenced numerous factors. Recent research indicates that the gut microbiota, along with its metabolites and associated immune-inflammatory responses, may contribute to the pathogenesis of PCOS. However, there is finite understanding of the potential intermediate impacts of circulating metabolites, immune cells, and inflammatory proteins on the linkage between intestinal flora and PCOS. This study seeks to explore the interrelationships among the gut microbiome, immune-inflammatory responses, and the metabolome within the context of PCOS.</p><p><strong>Methods: </strong>Aggregated statistics pertaining to individual traits were derived from genome-wide association studies accessible to general public. To elucidate mediation mechanisms, a two-step MR mediation framework was applied. In the first step, univariable MR was used to identify causal effects of gut microbial taxa on circulating metabolites, immune cells, and inflammatory proteins. In the second step, multivariable MR and mediation analyses were employed to estimate indirect effects of these mediators on PCOS. All analyses were performed using the inverse variance weighted (IVW) method as the primary model, complemented by MR-Egger, weighted median, and MR-PRESSO sensitivity tests.</p><p><strong>Results: </strong>MR analysis identified the causal impacts of 11 intestinal flora taxa on PCOS, among which 4 taxas (Class Betaproteobacteria, Genus Eubacterium eligens group, Genus Ruminiclostridium6 and Order Burkholderiales) may exert positive effects on PCOS, 7 taxas (Family Bifidobacteriaceae, Genus Bilophila, Genus Holdemania, Genus Hungatella, Genus Ruminococcaceae UCG004, Genus Veillonella, and Order Bifidobacteriales) may act as protective factors on PCOS. Meanwhile, 124 plasma metabolites, 45 circulating immune cell types, and 5 inflammatory proteins exhibited causal effects on PCOS. Notably, the circulating metabolites predominantly involved in PCOS were correlative of androgenic steroids, leucine, isoleucine and valine metabolism, glutathione metabolism, and secondary bile acid metabolism, and other metabolic pathways. Mediation analysis elucidated 34 metabolites-mediated path-ways linking gut microbiota and PCOS, with 13 immune cells/inflammatory proteins-mediated pathways interconnecting gut flora and PCOS.</p><p><strong>Conclusions: </strong>According to MR analysis, circulating metabolites, immune cells, and inflammatory proteins play a role in the correlation between intestinal flora and PCOS. The findings shed new light on the underlying pathophysiological mechanisms of PCOS.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148211710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed initiation of GnRH antagonist in PCOS patients undergoing IVF: a retrospective cohort study of premature ovulation and embryological outcomes.","authors":"Nuri Peker, Ayşen Yücetürk, Esra Tığlı, Özge Karaosmanoğlu, Burak Elmas, İlke Özer Aslan, Bülent Tıraş","doi":"10.1186/s13048-026-02161-3","DOIUrl":"10.1186/s13048-026-02161-3","url":null,"abstract":"<p><strong>Background: </strong>We aimed to evaluate whether delayed initiation of gonadotropin hormone-releasing hormone (GnRH) antagonist treatment affects premature ovulation and the number of good-quality blastocysts. We retrospectively reviewed the medical records of polycystic ovary syndrome (PCOS) patients who underwent in vitro fertilization (IVF) treatment at our clinic between 2017 and 2025. Patients were divided into two groups: Group 1: Patients in whom GnRH antagonist injections were initiated when at least three dominant follicles measured ≥17 mm. Group 2: Patients in whom GnRH antagonist was initiated upon identification of a single dominant follicle reaching ≥14 mm. The groups were compared in terms of premature ovulation, estradiol (E2) level at GnRH antagonist initiation, total number of retrieved oocytes, number and proportion of metaphase II (MII) oocytes, fertilization rate, blastocyst number and rate, and good-quality blastocyst grades.</p><p><strong>Results: </strong>A total of 89 patients were included in the study. Of these, 38 patients were assigned to Group 1 and 51 to Group 2. There were no statistically significant differences in age or body-mass index (BMI) between the groups. Serum E2 levels at GnRH antagonist initiation were significantly higher in Group 1. The \"median-(Interquartile Range (IQR))\" total oocyte count was 33 (27-41) in Group 1 and 30 (26-40) in Group 2. The median 2PN count was 19.5 (12.75-27) in Group 1 and 19.0 (15-22) in Group 2. There were no statistically significant differences between the groups in terms of MII oocyte or two pronuclei (2PN) count. Blastocyst outcomes were comparable between the groups; the median number of day 5-6 blastocysts was 7.0 (IQR: 5-11.5) in Group 1 and 8.0 (IQR: 5-11) in Group 2. Furthermore, the median number of Grade A blastocysts was 4.0 (IQR: 3-10) in Group 1 and 5.0 (IQR: 4-8) in Group 2, and there was no statistically significant difference between the groups regarding Grade‑A blastocyst counts. No premature ovulation occurred in either group.</p><p><strong>Conclusion: </strong>In this PCOS cohort, delayed GnRH antagonist initiation was not associated with impaired laboratory embryological outcomes or increased premature ovulation risk. Prospective randomized controlled trials are needed to confirm these preliminary findings.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13471402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circumventing IFN-γ induced resistance in ovarian cancer with a double-hit: NKG2A knockout of PRAME-TCR expressing NK cells.","authors":"Els P van Hees, Olivia W Rensing, Renate S Hagedoorn, Cilia R Pothast, Anne K Wouters, Rosa A van Amerongen, J H Frederik Falkenburg, Rosa de Groot, Mirjam H M Heemskerk","doi":"10.1186/s13048-026-02156-0","DOIUrl":"10.1186/s13048-026-02156-0","url":null,"abstract":"<p><strong>Background: </strong>The success of NK cell therapies against solid tumors remains limited, possibly due to tumor resistance mechanisms associated with the upregulation of inhibitory ligands. Previous studies have demonstrated that expanded NK cells can lyse ovarian cancer cells and produce IFN-γ. However, secretion of IFN-γ within the tumor microenvironment, leads to the upregulation of both classical HLA class I and the non-classical HLA-E on bystander tumor cells, thereby contributing to resistance against NK cell-mediated cytotoxicity. To overcome this IFN-γ induced resistance, we developed NKG2A-knockout NK: TCR cells targeting PRAME (NK: PRAME^{NKG2A KO}), a tumor-associated antigen expressed in ovarian cancer.</p><p><strong>Methods: </strong>Primary NK cells were isolated from PBMCs, stimulated with cytokines, and genetically modified using CRISPR-Cas9 to knockout the KLRC1 gene, which encodes NKG2A. After stimulation, the NK cells were further engineered to express the PRAME-specific TCR. NK: PRAME^{NKG2A KO} were compared with control NK: PRAME^{NKG2A WT} and NK: MOCK^{NKG2A KO} cells for effector function against PRAME-positive ovarian cancer cell lines and primary ovarian cancer cells. To mimic the pro-inflammatory tumor environment, ovarian cancer cell lines were pre-treated with IFN-γ.</p><p><strong>Results: </strong>First we observed that NK: PRAME cells without a KLRC1 knockout were not effective to lyse IFN-γ treated ovarian cancer cells, irrespective of upregulated HLA class I expression. To overcome HLA-E mediated inhibition, the CRISPR-Cas9 induced KLRC1 knockout was successfully achieved without negatively impacting NK: TCR cell engineering, expansion and further alterations in phenotype. As a result, the NK: PRAME^{NKG2A KO} cells exhibited increased cytotoxicity against these IFN-γ treated tumor cells.</p><p><strong>Conclusions: </strong>This dual-targeting strategy offers a unique advantage by enabling the targeting of both HLA-positive and HLA-negative tumor cells, promoting a pro-inflammatory environment and enhancing the efficacy of TCR-based immunotherapy for ovarian cancer and other solid tumors.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13464338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socio-demographic profiles, motivations and perspectives of women undergoing publicly funded elective fertility preservation: a nationwide multicenter study in France.","authors":"Jeanne Dufaure, Gaelle Marteil, Solène Vorilhon, Bruno Pereira, Cécily Lucas, Sarah Antunes, Anne-Sophie Gremeau, Florence Brugnon, Laure Chaput","doi":"10.1186/s13048-026-02157-z","DOIUrl":"10.1186/s13048-026-02157-z","url":null,"abstract":"<p><strong>Background: </strong>Elective fertility preservation (EFP) is publicly funded in France, but little data is available about the socio-demographic profiles and motivations of women opting for this procedure, as well as on their perspective and levels of knowledge on the subject. To address this issue, a national multicenter study was conducted within the French CECOS network using an anonymous online questionnaire between November 2023 and June 2024 to women who had started of completed EFP process.</p><p><strong>Results: </strong>A total of 351 responses were recorded. Most women requesting EFP were aged 33 years or older (87.2%), single (62.9%) and highly educated (95.4%). The main reason for choosing EFP treatment was the absence of a partner (50.7%). The main sources of information about EFP among women were their friends or family (37.6%), healthcare professionals (29.3%), and the media (27.1%). Regarding the future use of the cryopreserved oocytes, 68.5% of the women anticipated needing them. The intended family model primarily involved the use of cryopreserved oocytes within heterosexual couples (82.9%). Nevertheless, 42.6% of respondents also considered single motherhood. In the event of non-personal use, 65.8% of women would like to donate their oocytes. However, only 32.6% of women were aware of the French legal framework governing oocyte donation. Multiple correspondence analysis reveals two distinct clusters, which differ primarily in terms of fertility intentions and perspectives on oocyte use. Cluster 1 (48.1%, n = 169) comprises mainly single women who have completed the EFP procedure and wish to become pregnant and consider using their oocytes as single mothers. Conversely, Cluster 2 (44.7%, n = 157) comprises women with no prior experience of EFP, who were more likely to report a pregnancy despite not having immediate desire to become pregnant.</p><p><strong>Conclusions: </strong>This is the first French multicenter study examining EFP in women, three years after its authorization and reimbursement by the national healthcare system. Our study reveals two distinct profiles among women choosing EFP and emphasizes the need to improve counseling strategies to ensure they meet women's needs during this procedure.</p><p><strong>Trial registration: </strong>NCT07138469 (accepted on June 25, 2025 on ClinicalTrials.gov).</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perivitelline space volume and embryonic outcomes in conventional IVF: a single-center retrospective study with mixed-effects model analysis.","authors":"Xiaoqian Wang, Haojie Pan, Zitong Xu, Hai Wang, Xianjue Zheng, Chenhui Zhang, Shuqi Xia","doi":"10.1186/s13048-026-02159-x","DOIUrl":"10.1186/s13048-026-02159-x","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of oocyte perivitelline space volume on embryonic outcomes in conventional in vitro fertilization (IVF) cycles, and to provide an objective morphological quantitative index for evaluating oocyte quality.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 950 metaphase Ⅱ (MⅡ) oocytes from 110 conventional IVF cycles in the Reproductive Medicine Center of Wenzhou People's Hospital from January to December 2022. PVSV was measured by an image analysis system, and the oocytes were divided into four quartile groups (Q1: <25%, Q2: 25%-50%, Q3: 50%-75%, Q4: >75%) due to the non-normal distribution of PVSV data. The fertilization rate, normal fertilization rate, day-3 (D3) high-quality embryo rate, blastocyst formation rate and high-quality blastocyst rate were compared among the four groups. Mixed-effects models were applied to adjust for confounding factors and intra-patient clustering effects, and to analyze the correlation between PVSV and embryonic outcomes.</p><p><strong>Results: </strong>PVSV presented a slight right-skewed non-normal distribution, and 90.91% of the cycles contained oocytes from two or more quartile groups. No significant differences were found in fertilization rate and normal fertilization rate among the four groups (P > 0.05). The D3 high-quality embryo rate increased progressively with the elevation of PVSV, while the blastocyst formation rate and high-quality blastocyst rate peaked in Q2 and Q3 groups and decreased significantly in Q1 and Q4 groups (P < 0.05). Mixed-effects models confirmed a significant inverted U-shaped nonlinear correlation between PVSV and the above embryonic developmental indicators, and PVSV was an independent predictor of the in vitro developmental potential of post-fertilization embryos, with the optimal PVSV values clustering around the 3rd-4th deciles (corresponding to Q2-Q3).</p><p><strong>Conclusion: </strong>PVSV can serve as an objective and quantitative auxiliary indicator for laboratory-level oocyte quality evaluation in conventional IVF cycles, providing a reference for in vitro oocyte and embryo screening. This study only includes laboratory embryology outcomes and does not provide clinically meaningful outcomes such as implantation, clinical pregnancy, miscarriage, or live birth. Due to the limitations of single-center retrospective design and exclusive focus on in vitro laboratory outcomes, the clinical application value of PVSV, including its correlation with in vivo implantation, clinical pregnancy and live birth outcomes, needs to be further verified by multicenter prospective studies with comprehensive clinical follow-up data.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress.","authors":"Jinxin Zong, Yuxin Luo, Mengting Wu, Nan Wang, Yue Sui, Junjun Yang, Boqi Zhang, Chunjin Li, Xu Zhou","doi":"10.1186/s13048-026-02152-4","DOIUrl":"10.1186/s13048-026-02152-4","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) affects 1-5% of women under 40 years old and is characterized by granulosa cells (GCs) apoptosis and follicular atresia. However, the pathogenesis of POF is complex and lacks effective prevention and treatment strategies. N-carbamylglutamate (NCG), a bioactive substance known for its antioxidant properties; however, whether it can alleviate POF remains unclear.</p><p><strong>Methods: </strong>This study using the cyclophosphamide (Cy)-induced POF mouse model, and demonstrated that NCG, has beneficial effects in alleviating POF symptoms and restoring the intestinal mucosal barrier through gut microbiota-metabolite crosstalk. Integrated 16 S rRNA sequencing, untargeted metabolomics, and fecal bacteria transplantation (FMT) experiments revealed that NCG induced restructuring of gut microbial communities, with enrichment of Muribaculum intestinale and concomitant elevation of its associated Tryptophan-derived metabolite, Tryptophanol.</p><p><strong>Results: </strong>Mechanistically, Tryptophanol, supplementation alleviated POF by reducing mouse GCs apoptosis and oxidative stress via inhibiting Phosphatidylinositol 3-kinase/ Protein Kinase B (PI3K/AKT) and mitogen-activated protein kinase (MAPK) pathways, thereby restoring ovarian function and fertility, and the efficacy of NCG in alleviating POF relies on promoting Tryptophanol-mediated anti-oxidative stress.</p><p><strong>Conclusions: </strong>Our findings, highlight the \"gut microbiota-Tryptophanol-ovary\" axis in POF pathogenesis, and propose a potential therapy for NCG to regulate gut microbiota to restore ovarian redox homeostasis to alleviate POF.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}