Journal of Ovarian Research最新文献

{"title":"Salvia miltiorrhiza extract ameliorated ovarian damage induced by cyclophosphamide in mice by multidimensional mechanisms.","authors":"Lin Zhou, Hua-Lin Bai, Ling-Juan Wang, Juan Xiao, Yi Liu, Ji-Hui Ai, Ke-Zhen Li, Ding Ma, Yingyan Han","doi":"10.1186/s13048-025-01727-x","DOIUrl":"10.1186/s13048-025-01727-x","url":null,"abstract":"<p><strong>Introduction: </strong>Cyclophosphamide (CTX) can result in particularly severe ovarian damage by the broad range of damaging mechanisms, yet now single compounds struggle to provide comprehensive protection against CTX-induced ovarian damage. Salviae Miltiorrhizae Radix et Rhizoma (SM), as the traditional Chinese medicines (TCMs) with multi-component nature, offers hope for prevention and treatment against multi-mechanistic drugs causing ovarian damage. In this study, we aimed to investigate the protective effect of SM on ovarian hypofunction induced by CTX and explore its underlying mechanisms in mice.</p><p><strong>Methods: </strong>The Salvia miltiorrhiza Bunge with a final concentration of 1.33 g/mL was administrated daily by gavage in mice with tumor xenografts and/or CTX intraperitoneal intervention. The subcutaneous xenograft tumor volume of each mouse was recorded and calculated every 3 days by measuring its width and length. After drug administration, estrous cycle was monitored and follicle counting was performed by H&E staining, as well as ELISA assays for serum hormone and mating experiment for fertility assessment. The apoptosis inhibition, antioxidant capacity, vascular protective and anti-fibrotic effects of SM was respectively evaluated by TUNEL staining, ROS, DPPH scavenging and antioxidant enzymes assays, vascular morphological observation, masson staining, as well as immunofluorescence staining and western blotting for the related-protein expression.</p><p><strong>Results: </strong>SM didn't affect the anti-cancer efficacy of CTX and tumor growth rate in vivo. After SM administration for 41 days, the dropped body weight and decreased ovarian index caused by CTX gained obviously, the disturbed estrus cycle was markedly restored, a significant increase emerged in the levels of AMH and E₂ and a significant decrease appeared synchronously in FSH levels, a significant increase in primordial follicles and a corresponding decrease in atretic follicles was showed with the same increasing trend in the primary follicles, secondary follicles and antral follicles. Our results also showed that CTX could lead to pregnancy rate decreased, especially the average stillbirth rate raised obviously, both of which could be improved by SM, but without effect on the average litter size and body weight of alive pups. In-depth research showed that SM could effectively attenuate CTX-induced granulosa cells apoptosis to a certain extent by blocking the activation of the apoptotic proteins, also alleviate oxidative stress damage caused by CTX through its anti-oxidative stress ability, even reverse CTX-induced ovarian vascular occlusion and alleviate ovarian tissue fibrosis.</p><p><strong>Conclusion: </strong>Our findings verified that SM could significantly reduce primordial follicle (PMF) loss and follicular atresia, and ameliorate CTX-induced ovarian dysfunction and poor fertility, but didn't affect the anti-tumor effect of CTX an","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"149"},"PeriodicalIF":3.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular, cellular, biochemical, and rehabilitative insights into exercise interventions for gynecological cancer prevention and survivorship: a narrative review.","authors":"Yubing Wang, Melika Malek","doi":"10.1186/s13048-025-01725-z","DOIUrl":"10.1186/s13048-025-01725-z","url":null,"abstract":"<p><p>This review paper investigated existing literature on the relationship between physical activity, exercise, and gynecological cancers, focusing on ovarian, endometrial, and cervical cancers. Epidemiological studies consistently demonstrate an inverse association between physical activity levels and the risk of developing gynecological cancers, as well as improved survival outcomes among cancer survivors engaging in regular exercise. Mechanistic insights suggest that physical activity and exercise may modulate key pathways involved in carcinogenesis, tumor progression, and treatment response, offering promising avenues for preventive and adjunctive therapeutic interventions. However, despite a growing body of research in exercise oncology, there is a lack of disease-specific synthesis addressing how exercise can be tailored to the distinct pathophysiological, treatment-related, and psychosocial challenges of gynecological cancers. Current guidelines often generalize exercise recommendations across all cancer types, overlooking the unique symptom burdens, such as pelvic floor dysfunction, sarcopenia, and menopausal symptoms, specific to gynecologic malignancies. This review addresses this gap by examining the evidence base for exercise in ovarian, endometrial, and cervical cancers, with an emphasis on mechanistic, clinical, and rehabilitative dimensions. By integrating epidemiological findings with preclinical and intervention data, this work aims to establish a gynecologic-specific framework for exercise prescription. This review underscores the importance of multidisciplinary research efforts to optimize lifestyle interventions, inform evidence-based guidelines, and improve outcomes for individuals affected by gynecological cancers.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"147"},"PeriodicalIF":3.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of aqueous Stevia rebaudiana extract and clomiphene citrate on the expression of GDF9, PTEN, BMP15 genes, and antioxidant levels in a letrozole-induced polycystic ovary syndrome model in Wistar rats.","authors":"Alireza Saedi, Mehdi Mahmoodi, Reza Hosseiniara, Mahboubeh Vatanparast, Amir Hossein Fatehi Marj, Javad Paran Nejad, Moein Moradi, Sadegh Zarei","doi":"10.1186/s13048-025-01719-x","DOIUrl":"10.1186/s13048-025-01719-x","url":null,"abstract":"<p><p>This study investigates the individual and combined effects of aqueous Stevia rebaudiana extract (SAE) and clomiphene citrate (Clom) on the expression of key ovarian regulatory genes -growth differentiation factor 9 (GDF9), phosphatase and tensin homolog (PTEN), and bone morphogenetic protein 15 (BMP15)- as well as on antioxidant enzyme activities in a letrozole-induced polycystic ovary syndrome (PCOS) model in adult female Wistar rats. The estrous cycles of 50 rats were monitored and randomly divided into five groups of 10 rats each. The control group received carboxymethyl cellulose, while the remaining groups were administered letrozole (1 mg/kg) for 21 days to induce PCOS. From day 22 onward, the treatment groups received SAE (400 mg/kg), Clom (100 mg/kg), or their combination for 28 days. Gene expression levels of GDF9, BMP15, and PTEN were analyzed using real-time PCR, and antioxidant levels were assessed using diagnostic kits. Data were analyzed using SPSS software (version 21) with one-way ANOVA followed by Tukey's post hoc test. Treatments with SAE and Clom restored normal estrous cycles. Significant differences in gene expression were observed: PTEN levels were higher in the control group compared to SAE and combined groups, while GDF9 and BMP15 were elevated in the PCOS group compared to controls. SAE and Clom treatments also significantly increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in ovarian tissues. The combined SAE and Clom therapy demonstrate potential as an effective strategy to manage PCOS by regulating ovarian gene expression and oxidative stress.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"144"},"PeriodicalIF":3.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human endogenous retrovirus-K envelope protein is aberrantly expressed in serous ovarian cancer and promotes chemosensitivity via NF-κB/P-glycoprotein pathway inhibition.","authors":"Que Thanh Thanh Nguyen, Soo-Gui Shin, Tra Tran Thu Nguyen, Kyoung Yul Lee, Michael McClelland, Eun-Ju Lee","doi":"10.1186/s13048-025-01722-2","DOIUrl":"10.1186/s13048-025-01722-2","url":null,"abstract":"<p><strong>Background: </strong>Approximately 100,000 human endogenous retroviruses (HERV) are integrated into the human genome. Most HERVs are not expressed; however, transcription within the family HERV-K, a class II beta-retrovirus, occurs in specific cancers. Herein, we investigated HERV-K env protein expression and its clinical and molecular significance in serous ovarian cancer.</p><p><strong>Methods: </strong>Protein expression was assessed via immunohistochemistry and QuPath digital analysis in 24 normal, 10 benign, 13 borderline and 72 cancerous serous ovarian tissues. Clinicopathological parameters were obtained from medical records. Transcriptomes were evaluated using strand-specific reverse transcription-PCR and sequencing. Antiproliferative activities were explored using MTT and colony formation assays. A stable transfection expression system and siRNA gene silencing were used. Resistant cell lines were established using the paclitaxel concentration gradient method and chemoresponsiveness was evaluated by measuring the IC₅₀.</p><p><strong>Results: </strong>HERV-K env was absent in normal ovarian epithelia and benign tumors but was detected in 37.5% (27 of 72) of serous carcinomas and 61.5% (8 of 13) of borderline tumors. Unexpectedly, HERV-K env was observed in lymphocytes only in a subset of HERV-K env-positive tumors: 18 of 27 invasive and 1 of 8 borderline tumors. HERV-K env-positivity was significantly associated with chemosensitivity, although the prognosis was unaffected. HERV-K type I (K101, K102, and K103) and II (K108 and K115) were transcribed in cultured ovarian cancer cells and tissues but not in their paclitaxel-resistant derivatives. Forced expression of HERV-K env in paclitaxel-resistant cells suppressed cellular proliferation and resensitized cells to paclitaxel by inhibiting NF-κB/P-glycoprotein. siRNA-mediated HERV-K env knockdown restored paclitaxel-resistance by recovering NF-κB/P-glycoprotein.</p><p><strong>Conclusion: </strong>HERV-K env was expressed in serous ovarian carcinoma and significantly associated with chemosensitivity. HERV-K env attenuated NF-κB/P-glycoprotein, a mechanism of chemoresistance. Hence, it could have therapeutic potential in chemoresistant ovarian cancers.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"146"},"PeriodicalIF":3.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New perspectives on polycystic ovary syndrome: hypothalamic-sympathetic-adipose tissue interaction.","authors":"Shinan Zhang, Ningxiao Jiang, Guo Liu, Baolin Zhang, Huishu Xu, Yingjiang Xu, Gaofeng Qin, Yanlin Wang, Lei Zhang, Boping Yang, Lei Han","doi":"10.1186/s13048-025-01724-0","DOIUrl":"10.1186/s13048-025-01724-0","url":null,"abstract":"<p><p>Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine and metabolic disorder among women of reproductive age. Although the traditional mechanistic framework has focused on dysregulation of the hypothalamic-pituitary-ovarian axis (HPOA), emerging evidence suggests that PCOS-associated metabolic disturbances predominantly originate from dysregulation of the hypothalamic-sympathetic nerve-adipose tissue axis (HSF axis). This article systematically reviews the mechanisms by which hypothalamic nuclei (including the arcuate nucleus and paraventricular nucleus) regulate adipose tissue metabolism through sympathetic neural pathways. Furthermore, it explores how HSF axis dysfunction exacerbates both metabolic and reproductive abnormalities in PCOS through pathophysiological pathways such as chronic low-grade inflammation and energy sensing dysregulation. This synthesis offers novel insights into PCOS pathogenesis by bridging the gap between neuroendocrine regulation and systemic metabolic dysfunction.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"145"},"PeriodicalIF":3.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of cumulative live birth rates in patients with recurrent pregnancy loss undergoing preimplantation genetic testing for aneuploidy versus conventional in vitro fertilisation/intracytoplasmic sperm injection: a retrospective study.","authors":"Pei Xin Li, Jing Yi Han, Wei Zheng, Ran Shen, Nan Meng, Bing Nan Ren, Yi Xuan Yan, Yue Yang, Man Yu Hu, Yi Chun Guan","doi":"10.1186/s13048-025-01721-3","DOIUrl":"10.1186/s13048-025-01721-3","url":null,"abstract":"<p><strong>Background: </strong>Recurrent pregnancy loss (RPL) affects 1-2% of women worldwide and poses diagnostic and therapeutic challenges due to its multifactorial causes. Preimplantation genetic testing for aneuploidy (PGT-A) aims to improve outcomes by selecting euploid embryos, but its benefits in RPL patients remain uncertain. This study compared the effectiveness of PGT-A versus conventional in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI) in improving cumulative live birth rates (CLBRs) and explored the effects of maternal age and miscarriage frequency on treatment efficacy.</p><p><strong>Methods: </strong>This study included RPL patients who underwent their first oocyte retrieval and at least one single-blastocyst transfer between June 2016 and June 2022. Patients were divided into an IVF/ICSI group (n = 156) and a PGT-A group (n = 198). Primary outcomes included the CLBR, live birth rate, miscarriage rate, time to live birth, and perinatal outcomes.</p><p><strong>Results: </strong>After three single-blastocyst transfer cycles, no significant difference was observed in the conservative CLBR between the PGT-A and IVF/ICSI groups (Cycle 1: adjusted odds ratio [aOR] = 0.78, 95% confidence interval [CI]: 0.49-1.23; Cycle 2: aOR = 0.81, 95% CI: 0.51-1.29; Cycle 3: aOR = 0.96, 95% CI: 0.60-1.53; all P > 0.05). Similarly, the optimal CLBR after three transfer cycles showed no significant difference between the two groups (P > 0.05). However, the time to live birth was significantly longer in the PGT-A group than in the IVF/ICSI group (adjusted hazard ratio = 0.56, 95% CI: 0.42-0.75, P < 0.05). Other outcomes were comparable between the two groups.</p><p><strong>Conclusion: </strong>PGT-A did not significantly improve the CLBR or shorten the time to live birth in RPL patients. Further research is needed to elucidate its role and identify potential subgroups within the RPL population that may benefit from PGT-A.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"141"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful treatment of Heyan Kuntai capsule combined with hormonal therapy in an adolescent with diminished ovarian reserve: a case report.","authors":"Xue Ao, Ling Xiong, Jialu Zhu, Shiqiao Tan","doi":"10.1186/s13048-025-01716-0","DOIUrl":"10.1186/s13048-025-01716-0","url":null,"abstract":"<p><strong>Background: </strong>Diminished ovarian reserve (DOR), a common female disease, results in reduced fertility and menstrual disorders. Patients without timely treatment often suffer from infertility, ovarian insufficiency, premature ovarian failure and early onset or worsened perimenopausal symptoms caused by estrogen fluctuation or deficiency. However, there is a lack of effective methods to improve ovarian reserve function.</p><p><strong>Case presentation: </strong>Here, we report a case of a 13-year-old female with 2-year menstrual disorder. The anti-mullerian hormone (AMH) levels were test twice, with interval of 2 months, yielding results of 0.41 ng/mL and 0.50 ng/mL, respectively. Colour Doppler ultrasound examination showed that there were 4-6 antral follicles bilaterally. The patient was diagnosed with DOR. She was treated with Heyan Kuntai capsule (HYKT) combined with hormonal therapy (HT). Post treament, her AMH level, follicle stimulating hormone level and AFC normalized. Discontinuation of HYKT and continuation of HT alone for 5 months proved ineffective. Consequently, HYKT in combination with HT was continued, leading to normal ovarian reserve function restoration. No adverse reactions were observed during the 4-year follow-up.</p><p><strong>Conclusions: </strong>This case highlights the importance of including DOR as a diagnostic consideration in adolescents with menstrual disorders during clinical evaluation. AND combining HYKT with HT might contribute to potential improvements in ovarian function in DOR patients.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"143"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of hyperuricemia with higher miscarriage rates and lower live birth rates in women undergoing IVF/ICSI.","authors":"Ling Zhou, Jiahuan Luo, Shuhua Zhao, Longda Wang, Meng Rao, Huawei Wang, Li Tang","doi":"10.1186/s13048-025-01720-4","DOIUrl":"10.1186/s13048-025-01720-4","url":null,"abstract":"<p><strong>Background: </strong>Hyperuricemia (HUA) has become popular globally, being an important risk factor for various metabolic diseases. Elevated serum uric acid (UA) levels cause adverse reproductive outcomes in women with polycystic ovary syndrome undergoing assisted reproductive technology (ART). However, its impact on reproductive outcomes in the general population is unknown.</p><p><strong>Method: </strong>This retrospective study was conducted on a general population of infertility patients at a single center (March 2016-April 2023). Overall, 2189 first transfer cycles were screened for inclusion. HUA was defined as serum UA ≥ 360 µmol/L. Variables identified by LASSO regression analysis were entered into logistic regression models to calculate odds ratios. Generalized additive models were employed to examine the nonlinear relationship between serum UA as a continuous variable with outcomes. The primary outcome was live birth rate (LBR).</p><p><strong>Results: </strong>Baseline characteristics revealed that HUA patients presented with significantly elevated metabolic parameters, including higher BMI, fasting glucose, lipid profiles, and greater prevalence of polycystic ovarian syndrome. Patients with HUA demonstrated significantly lower LBR and fertilization rates, along with higher miscarriage rates, while no significant differences were observed in oocyte retrieval numbers, embryo utilization rates, high-quality cleavage embryo formation, blastocyst formation rates, or clinical pregnancy rates (CPR). After adjusting for confounding variables, HUA remained a significant factor affecting LBR and miscarriage rate. Notably, the detrimental effects of HUA exhibited modality-specific patterns, with frozen-thawed embryo transfer (FET) cycles demonstrating greater vulnerability to HUA than fresh transfers. Among younger women, HUA independently predicted reduced LBR and increased miscarriage risk, with no significant association observed in those ≥ 35 years. And this effect remained significant in normal-weight (< 24 kg/m²) women but not in overweight individuals.</p><p><strong>Conclusion: </strong>Elevated UA levels are linked to lower LBR and higher miscarriage risk in ART, especially in younger, non-obese women where HUA is an independent risk factor. Though not affecting embryo quality or implantation, HUA may impair pregnancy maintenance. Even in older or overweight patients, UA monitoring remains important. Routine assessment and tailored management-particularly greater attention to pregnancy loss and cautious use of programmed FET in poorly controlled cases-may help improve ART outcomes.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"142"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIB3 regulates Cx43 expression in human granulosa cells via the Wnt/β-catenin signaling pathway: an in vitro study.","authors":"Zhoujun Zhu, Jiaoyun Dong, Wei Liu, Lan Xia, Zhihong Niu","doi":"10.1186/s13048-025-01694-3","DOIUrl":"10.1186/s13048-025-01694-3","url":null,"abstract":"<p><strong>Background: </strong>Increased expression of tribbles pseudokinase 3 (TRIB3), which is attributed to increased levels of free fatty acids in the follicular fluid of women with obesity, is correlated with reduced oocyte developmental competence. Connexin 43 (Cx43) is a crucial element of gap junctions in human ovarian granulosa cells (GCs) and facilitates oocyte development. This study aimed to investigate whether high TRIB3 expression and decreased oocyte development potential is regulated by aberrant Cx43 expression in human GCs under high fatty acid culture conditions.</p><p><strong>Methods: </strong>GCs from women undergoing in vitro fertilization were grouped by body mass index (BMI). The human granulosa-like tumor cell line (KGN) was exposed to palmitic acid (PA), and its effect on cell apoptosis, viability and proliferation was assessed. The effects of TRIB3 overexpression and knockdown on KGN cells were analyzed. Gene and protein expression, along with the activity of the Wnt/β-catenin signaling pathway, were analyzed using quantitative reverse transcription-PCR, western blotting, immunofluorescence staining, and co-immunoprecipitation. Gap junction function was assessed using a scrape loading/dye transfer assay.</p><p><strong>Results: </strong>Fertilization rates declined in women with overweight or obesity. In the GCs of these women, TRIB3 and Cx43 were upregulated. The protein expression of TRIB3 and Cx43 increased in KGN cells exposed to PA. TRIB3 overexpression led to increased Cx43 expression, whereas TRIB3 knockdown resulted in reduced Cx43 expression. Further mechanistic investigations revealed that TRIB3 upregulated Cx43 by reducing the degradation of β-catenin in the Wnt signaling pathway.</p><p><strong>Conclusions: </strong>TRIB3 overexpression can enhance Cx43 expression in human GCs by inhibiting the Wnt/β-catenin pathway, thereby reducing oocyte maturation rate. These findings could be used to improve the quality of female oocytes and enhance infertility therapy in the clinic.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"139"},"PeriodicalIF":3.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a single-center predictive model for conventional in vitro fertilization outcomes excluding total fertilization failure: implications for protocol selection.","authors":"Hai Wang, Haojie Pan, Zitong Xu, Xianjue Zheng, Shuqi Xia, Jiayong Zheng","doi":"10.1186/s13048-025-01728-w","DOIUrl":"10.1186/s13048-025-01728-w","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a multidimensional clinical indicator-based prediction model for identifying high-risk patients with fertilization failure conventional in vitro fertilization (c-IVF) cycles, thereby optimizing therapeutic decision-making.</p><p><strong>Methods: </strong>This retrospective single-center study analyzed 691 cycles (594 c-IVF, 97 rescue ICSI) from January 2019 to August 2024. Key parameters included female age, BMI, male semen parameters (sperm concentration, total progressive motile sperm count [TPMC], DNA fragmentation index [DFI]), and infertility duration. Three machine learning models (logistic regression, random forest, XGBoost) were developed and validated using a nested cross-validation framework with SMOTE oversampling.</p><p><strong>Results: </strong>The logistic regression model demonstrated superior predictive performance (mean AUC = 0.734 ± 0.049), significantly outperforming random forest (0.714 ± 0.034) and XGBoost (0.697 ± 0.038). Significant predictors included protective factors-male age (OR = 0.642, 95%CI:0.598-0.689) and TPMC (OR = 0.428, 95%CI:0.392-0.466), and risk factors-female BMI (OR = 1.268, 95%CI:1.191-1.351) and DFI (OR = 1.362, 95%CI:1.274-1.455). The nomogram showed moderate-to-high discriminative power (C-index = 0.722, 95%CI:0.667-0.773) upon internal validation. Decision curve analysis confirmed clinical utility at threshold probabilities between 0.05 and 0.60.</p><p><strong>Conclusions: </strong>The logistic regression-based prediction model exhibits robust performance in assessing c-IVF fertilization failure risk. While optimized for our center's specific clinical context, external multicenter validation is required to confirm broader clinical applicability.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"138"},"PeriodicalIF":3.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12220611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}