Journal of Ovarian Research最新文献

{"title":"Socio-demographic profiles, motivations and perspectives of women undergoing publicly funded elective fertility preservation: a nationwide multicenter study in France.","authors":"Jeanne Dufaure, Gaelle Marteil, Solène Vorilhon, Bruno Pereira, Cécily Lucas, Sarah Antunes, Anne-Sophie Gremeau, Florence Brugnon, Laure Chaput","doi":"10.1186/s13048-026-02157-z","DOIUrl":"10.1186/s13048-026-02157-z","url":null,"abstract":"<p><strong>Background: </strong>Elective fertility preservation (EFP) is publicly funded in France, but little data is available about the socio-demographic profiles and motivations of women opting for this procedure, as well as on their perspective and levels of knowledge on the subject. To address this issue, a national multicenter study was conducted within the French CECOS network using an anonymous online questionnaire between November 2023 and June 2024 to women who had started of completed EFP process.</p><p><strong>Results: </strong>A total of 351 responses were recorded. Most women requesting EFP were aged 33 years or older (87.2%), single (62.9%) and highly educated (95.4%). The main reason for choosing EFP treatment was the absence of a partner (50.7%). The main sources of information about EFP among women were their friends or family (37.6%), healthcare professionals (29.3%), and the media (27.1%). Regarding the future use of the cryopreserved oocytes, 68.5% of the women anticipated needing them. The intended family model primarily involved the use of cryopreserved oocytes within heterosexual couples (82.9%). Nevertheless, 42.6% of respondents also considered single motherhood. In the event of non-personal use, 65.8% of women would like to donate their oocytes. However, only 32.6% of women were aware of the French legal framework governing oocyte donation. Multiple correspondence analysis reveals two distinct clusters, which differ primarily in terms of fertility intentions and perspectives on oocyte use. Cluster 1 (48.1%, n = 169) comprises mainly single women who have completed the EFP procedure and wish to become pregnant and consider using their oocytes as single mothers. Conversely, Cluster 2 (44.7%, n = 157) comprises women with no prior experience of EFP, who were more likely to report a pregnancy despite not having immediate desire to become pregnant.</p><p><strong>Conclusions: </strong>This is the first French multicenter study examining EFP in women, three years after its authorization and reimbursement by the national healthcare system. Our study reveals two distinct profiles among women choosing EFP and emphasizes the need to improve counseling strategies to ensure they meet women's needs during this procedure.</p><p><strong>Trial registration: </strong>NCT07138469 (accepted on June 25, 2025 on ClinicalTrials.gov).</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perivitelline space volume and embryonic outcomes in conventional IVF: a single-center retrospective study with mixed-effects model analysis.","authors":"Xiaoqian Wang, Haojie Pan, Zitong Xu, Hai Wang, Xianjue Zheng, Chenhui Zhang, Shuqi Xia","doi":"10.1186/s13048-026-02159-x","DOIUrl":"10.1186/s13048-026-02159-x","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of oocyte perivitelline space volume on embryonic outcomes in conventional in vitro fertilization (IVF) cycles, and to provide an objective morphological quantitative index for evaluating oocyte quality.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 950 metaphase Ⅱ (MⅡ) oocytes from 110 conventional IVF cycles in the Reproductive Medicine Center of Wenzhou People's Hospital from January to December 2022. PVSV was measured by an image analysis system, and the oocytes were divided into four quartile groups (Q1: <25%, Q2: 25%-50%, Q3: 50%-75%, Q4: >75%) due to the non-normal distribution of PVSV data. The fertilization rate, normal fertilization rate, day-3 (D3) high-quality embryo rate, blastocyst formation rate and high-quality blastocyst rate were compared among the four groups. Mixed-effects models were applied to adjust for confounding factors and intra-patient clustering effects, and to analyze the correlation between PVSV and embryonic outcomes.</p><p><strong>Results: </strong>PVSV presented a slight right-skewed non-normal distribution, and 90.91% of the cycles contained oocytes from two or more quartile groups. No significant differences were found in fertilization rate and normal fertilization rate among the four groups (P > 0.05). The D3 high-quality embryo rate increased progressively with the elevation of PVSV, while the blastocyst formation rate and high-quality blastocyst rate peaked in Q2 and Q3 groups and decreased significantly in Q1 and Q4 groups (P < 0.05). Mixed-effects models confirmed a significant inverted U-shaped nonlinear correlation between PVSV and the above embryonic developmental indicators, and PVSV was an independent predictor of the in vitro developmental potential of post-fertilization embryos, with the optimal PVSV values clustering around the 3rd-4th deciles (corresponding to Q2-Q3).</p><p><strong>Conclusion: </strong>PVSV can serve as an objective and quantitative auxiliary indicator for laboratory-level oocyte quality evaluation in conventional IVF cycles, providing a reference for in vitro oocyte and embryo screening. This study only includes laboratory embryology outcomes and does not provide clinically meaningful outcomes such as implantation, clinical pregnancy, miscarriage, or live birth. Due to the limitations of single-center retrospective design and exclusive focus on in vitro laboratory outcomes, the clinical application value of PVSV, including its correlation with in vivo implantation, clinical pregnancy and live birth outcomes, needs to be further verified by multicenter prospective studies with comprehensive clinical follow-up data.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress.","authors":"Jinxin Zong, Yuxin Luo, Mengting Wu, Nan Wang, Yue Sui, Junjun Yang, Boqi Zhang, Chunjin Li, Xu Zhou","doi":"10.1186/s13048-026-02152-4","DOIUrl":"10.1186/s13048-026-02152-4","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) affects 1-5% of women under 40 years old and is characterized by granulosa cells (GCs) apoptosis and follicular atresia. However, the pathogenesis of POF is complex and lacks effective prevention and treatment strategies. N-carbamylglutamate (NCG), a bioactive substance known for its antioxidant properties; however, whether it can alleviate POF remains unclear.</p><p><strong>Methods: </strong>This study using the cyclophosphamide (Cy)-induced POF mouse model, and demonstrated that NCG, has beneficial effects in alleviating POF symptoms and restoring the intestinal mucosal barrier through gut microbiota-metabolite crosstalk. Integrated 16 S rRNA sequencing, untargeted metabolomics, and fecal bacteria transplantation (FMT) experiments revealed that NCG induced restructuring of gut microbial communities, with enrichment of Muribaculum intestinale and concomitant elevation of its associated Tryptophan-derived metabolite, Tryptophanol.</p><p><strong>Results: </strong>Mechanistically, Tryptophanol, supplementation alleviated POF by reducing mouse GCs apoptosis and oxidative stress via inhibiting Phosphatidylinositol 3-kinase/ Protein Kinase B (PI3K/AKT) and mitogen-activated protein kinase (MAPK) pathways, thereby restoring ovarian function and fertility, and the efficacy of NCG in alleviating POF relies on promoting Tryptophanol-mediated anti-oxidative stress.</p><p><strong>Conclusions: </strong>Our findings, highlight the \"gut microbiota-Tryptophanol-ovary\" axis in POF pathogenesis, and propose a potential therapy for NCG to regulate gut microbiota to restore ovarian redox homeostasis to alleviate POF.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety assessment of autologous ovarian tissue cryopreservation and transplantation in cancer patients: an updated review.","authors":"Luyao Wang, Yifan Chu, Jiaxin Xie, Jiayun Chen, Yetan Liu, Shulin Yang, Ying Zhang, Jing Yue","doi":"10.1186/s13048-026-02155-1","DOIUrl":"10.1186/s13048-026-02155-1","url":null,"abstract":"<p><p>Recently, the global incidence of malignant tumors has been increasing with a notable trend toward younger patients, representing a major public health challenge. Fertility preservation has emerged as a key issue at the intersection of oncology and reproductive medicine. Ovarian tissue cryopreservation and transplantation (OTCT) offers a unique and valuable strategy, particularly for prepubertal girls and patients requiring immediate gonadotoxic therapy. Although OTCT can effectively restore ovarian endocrine function and fertility, its clinical application is largely limited by safety concerns, especially the potential risk of reintroducing viable malignant cells via transplanted ovarian tissue. Therefore, accurate detection of minimal residual disease (MRD) in cryopreserved ovarian tissue and effective tumor cell decontamination strategies are essential to ensure clinical safety. In addition, long-term follow-up data on the health status of offspring born after OTCT remain insufficient. This narrative review systematically summarizes the safety concerns of OTCT, focusing on cancer-specific ovarian tissue contamination risks, current MRD detection and purging techniques, as well as the intergenerational safety of offspring. Based on recent evidence, this review can further provide key conclusions and practical recommendations for the safety assessment of OTCT, with the aim of promoting the safe, standardized, and widespread clinical application of this technique.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic value of the Oocyte Maturation Index (OMI) on pregnancy outcomes in POSEIDON group 3 and 4 patients: a retrospective cohort study.","authors":"Yakup Bayram, Hamza Yildiz, Gorkem Ulger, Huseyin Durukan, Devrim Tok, Faik Gurkan Yazici","doi":"10.1186/s13048-026-02141-7","DOIUrl":"10.1186/s13048-026-02141-7","url":null,"abstract":"<p><strong>Background: </strong>This study METHODS: A total of 662 patients treated at the Assisted Reproductive Techniques Unit of Mersin University Faculty of Medicine between 2016 and 2024 (Group 3: n=245; Group 4: n=417) were retrospectively analyzed. Patients were divided into three subgroups based on OMI values: ≤50%, 51-75%, and >75%.</p><p><strong>Results: </strong>In both POSEIDON groups, the numbers of aspirated follicles, retrieved oocytes, MII oocytes, and high-quality day-3 embryos showed significant differences between OMI groups (p<0.001). In POSEIDON Group 3, patients with an OMI of 51-75% achieved the highest rates of biobiochemical pregnancy (43.9%), clinical pregnancy (42.1%), and live birth (35.1%), while these rates were lowest in the group with an OMI of ≤50% (10.9%, 10.9%, and 5.5%, respectively; p=0.001). In POSEIDON Group 4, while OMI significantly influenced chemical (p=0.005) and clinical pregnancy (p=0.014) rates, it did not result in a statistically significant difference in live birth rates (p=0.056).</p><p><strong>Conclusion: </strong>OMI is a clinically significant prognostic parameter, particularly for younger patients in the POSEIDON Group 3. In older patients (Group 4), however, age-related oocyte proglems my controbite the decisive effect of OMI on live birth. Incorporating OMI into routine clinical evaluations may contribute to personalized treatment planning and patient counseling.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148156883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of HDL-cholesterol related inflammatory ratios with reproductive outcomes in women with polycystic ovary syndrome undergoing frozen-thawed embryo transfer.","authors":"Xiaoyu Zhang, Jun'an Yu, Qunyan Zhu, Ze Ren, Siqi Wen, Xuefeng Huang, Zhiyang Zhou, Sisi Zhang","doi":"10.1186/s13048-026-02145-3","DOIUrl":"10.1186/s13048-026-02145-3","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder and a major cause of anovulatory infertility, often requiring assisted reproductive technology (ART). The freeze-all strategy followed by frozen-thawed embryo transfer (FET) can reduce the risk of ovarian hyperstimulation syndrome (OHSS) and improve endometrial receptivity. However, women with PCOS still experience suboptimal reproductive outcomes. Increasing evidence suggests that chronic low-grade inflammation may contribute to these impairments. Conventional inflammatory markers, such as circulating cytokines, are not routinely assessed in clinical practice, underscoring the need for simple and accessible indicators. Recently, several hematologic and lipid-based inflammatory ratios-lymphocyte-to-HDL cholesterol ratio (LHR), neutrophil-to-HDL cholesterol ratio (NHR), platelet-to-HDL cholesterol ratio (PHR) and monocyte-to-HDL cholesterol ratio (MHR)-have been proposed as accessible indicators of systemic inflammation. Nevertheless, their predictive value for ART outcomes in women with PCOS remains unclear.</p><p><strong>Methods: </strong>This retrospective cohort study included 1445 women with PCOS undergoing their first frozen embryo transfer cycle at the reproductive medicine center of a tertiary hospital between 2010 and 2023. The associations of reproductive outcomes with NHR, LHR, PHR and MHR were assessed using logistic regression models with or without adjusting for potential confounders.</p><p><strong>Results: </strong>Higher quartiles of NHR, LHR, and PHR were associated with increased miscarriage rates and decreased live birth rates, whereas no consistent pattern was observed for MHR. No significant differences were found across quartiles in biochemical pregnancy, clinical pregnancy, preterm delivery and low birth weight. Compared with women in the lowest quartile, those in the highest quartile had significantly higher odds of miscarriage for NHR (adjusted odds ratio [OR] 2.51, 95% confidence interval [CI] 1.39-4.63), LHR (2.32, 1.23-4.49) and PHR (2.47, 1.34-4.64). In addition, LHR and PHR were inversely associated with live birth after full adjustment.</p><p><strong>Conclusion: </strong>Elevated NHR, LHR, and PHR were associated with adverse reproductive outcomes in women with PCOS undergoing FET. These findings suggest that HDL-C related inflammatory ratios may have potential value as accessible markers of systemic inflammation in this population. However, given the retrospective design, their clinical utility for risk stratification and individualized management requires further validation in prospective studies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148156897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis and chemotherapy resistance in ovarian cancer: molecular mechanisms and therapeutic opportunities.","authors":"Shuqing Li, Lina Yang, Zhiling Zhu","doi":"10.1186/s13048-026-02150-6","DOIUrl":"10.1186/s13048-026-02150-6","url":null,"abstract":"<p><p>Ovarian cancer remains the most lethal gynecologic malignancy, with acquired platinum resistance accounting for the majority of treatment failures. Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation, has emerged as a metabolic vulnerability that therapy-resistant cancers cannot fully evade. This review examines the relationship between ferroptosis and chemotherapy resistance in high-grade serous ovarian cancer (HGSOC), analyzing the GPX4 antioxidant axis, polyunsaturated fatty acid biosynthesis, and iron homeostasis. We highlight recent discoveries including BRCA1-dependent ubiquitination of GPX4 and its implications for PARP inhibitor synthetic lethality, NR1D2-mediated transcriptional repression of FSP1, and stromal protection conferred by cancer-associated fibroblasts. The unique features of ovarian cancer-iron-abundant ascites, the lipid-rich omental niche, and prevalent BRCA mutations-present distinctive therapeutic opportunities. We introduce the novel concepts of a \"ferroptosis compensatory threshold\" requiring concurrent multi-node inhibition, and the need for personalized therapeutic design based on tumor ferroptosis defense profiling. We also address challenges confronting clinical translation: on-target toxicities, resistance mechanisms, spatial heterogeneity in ferroptosis susceptibility, and the need for validated predictive biomarkers. By synthesizing these cutting-edge findings, we provide a framework that integrates cancer genetics, microenvironment metabolism, and immunology, distinguishing this review from previous summaries and highlighting actionable vulnerabilities for overcoming chemoresistance in ovarian cancer.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13281579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148149016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the association between abnormal body mass index and oocyte quality.","authors":"Dan Lin, Yang-Zi-Yu Tao, Xin-Xin Lin, Er-Juan Lin, Bing-Bing Pan, Ming-Ting Jiang, Zhi-Ren Liu","doi":"10.1186/s13048-026-02153-3","DOIUrl":"10.1186/s13048-026-02153-3","url":null,"abstract":"<p><p>In recent years, the global prevalence of obesity has witnessed a sharp increase. Abnormal body mass index (BMI) exerts a profound influence on female fertility, particularly on oocyte quality. This review systematically summarizes the correlation between abnormal BMI and oocyte quality. It focuses on the mechanisms through which obesity impairs oocytes by disrupting lipid metabolism, energy supply, oxidative stress, DNA damage and repair, as well as epigenetic regulation. Obesity disrupts lipid homeostasis in follicular fluid, thereby inducing lipotoxicity. It damages mitochondria and the endoplasmic reticulum, resulting in insufficient energy production. It triggers excessive oxidative stress, which impairs mitochondrial DNA integrity and meiosis. It aggravates DNA damage while weakening the repair capacity, and disturbs DNA methylation and histone modifications, thus posing a threat to oocyte genomic stability and the developmental health of offspring. Although low BMI is also associated with poor oocyte quality, relevant studies are scarce, limiting the coverage of low BMI in this review. Clinically, abnormal BMI reduces the rates of implantation and live birth and increases the risk of miscarriage. Future research ought to focus on the mechanisms by which obesity and underweight affect oocyte quality, formulate targeted interventions like weight management and antioxidant therapy, and refine personalized assisted reproductive strategies to enhance the reproductive outcomes of women with abnormal BMI.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOMA-IR partially mediates the association between percent appendicular skeletal muscle mass and IVF/ICSI outcomes.","authors":"Danyu Ni, Xinyu Wang, Zichen Zheng, Kaidi Yu, Qijun Xie, Chun Zhao, Xiufeng Ling, Ye Yang","doi":"10.1186/s13048-026-02154-2","DOIUrl":"10.1186/s13048-026-02154-2","url":null,"abstract":"<p><strong>Background: </strong>Skeletal muscle mass contributes to metabolic health, particularly in relation to insulin sensitivity, and both skeletal muscle mass and metabolic health may influence outcomes after assisted reproductive technology (ART). However, whether insulin sensitivity partially explains the association between skeletal muscle mass and ART outcomes remains unclear. This study aimed to investigate the association between the percentage of appendicular skeletal muscle mass (PASM) and outcomes from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) and to explore whether insulin resistance, assessed by HOMA-IR, partially mediates this association.</p><p><strong>Methods: </strong>This retrospective study included 990 patients who underwent IVF/ICSI at Nanjing Medical University from April 2022 to April 2023. PASM was measured by bioelectrical impedance analysis (BIA) and analysed both as quartiles and as a continuous variable. Insulin sensitivity was assessed using the homeostatic model assessment of insulin resistance (HOMA‑IR). We employed Spearman's correlation analysis, multivariate regression, restricted cubic spline (RCS) models, and mediation analysis with adjustment for relevant confounders.</p><p><strong>Results: </strong>Unadjusted comparisons showed only non-significant increasing trends across PASM quartiles, whereas adjusted regression analyses indicated more favourable laboratory outcomes in higher PASM quartiles. Each 1-unit increase in PASM was associated with the number of oocytes retrieved (Beta = 0.29), available embryos (Beta = 0.25) and blastocysts (Beta = 0.21), as well as higher available embryo rate (Beta = 1.49) and blastocyst formation rate (Beta = 1.52). PASM was positively linked to cumulative clinical pregnancy rate (CCPR) (OR = 1.30). Higher PASM was inversely associated with HOMA‑IR and insulin resistance (IR) prevalence; elevated HOMA‑IR was independently associated with poorer embryology laboratory indicators (P < 0.05). Mediation analysis suggested that HOMA-IR modestly mediated 5.5%, 5.9%, and 7.3% of the associations between PASM and the number of oocytes retrieved, available embryos and blastocysts, respectively.</p><p><strong>Conclusions: </strong>Higher PASM was associated with more favourable embryology laboratory indicators and higher pregnancy success after IVF/ICSI, with HOMA-IR showing partial and modest mediation. Interventions targeting skeletal muscle mass may represent a potential strategy for optimizing assisted reproduction outcomes, pending confirmation in prospective studies.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13436113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The improvement of umbilical cord mesenchymal stem cells on the ovarian microenvironment: a systematic review and meta-analysis.","authors":"Kailu Zhao, Yanan Wu, Jinwei Yang, Kehu Yang, Xue Fan, Yanhua Zhang","doi":"10.1186/s13048-026-02148-0","DOIUrl":"10.1186/s13048-026-02148-0","url":null,"abstract":"<p><strong>Objective: </strong>To systematically evaluate the effects of umbilical cord mesenchymal stem cells (UCMSCs) on the ovarian microenvironment in mouse models of premature ovarian failure (POF).</p><p><strong>Methods: </strong>A comprehensive search was conducted in Wanfang, VIP, CNKI, PubMed, Web of Science, Embase, and Cochrane Library databases for randomized controlled animal trials on the transplantation of mesenchymal stem cells for treating the ovarian microenvironment. The search period was from the establishment of the databases to September 19, 2025. Two researchers independently screened the literature, extracted data, and evaluated the methodological quality using the SYRCLE risk of bias tool for animal experiments. Quantitative data were analyzed using RevMan 5.4.1 software.</p><p><strong>Results: </strong>A total of 10 randomized controlled animal studies were included. The meta-analysis results showed that the transplantation of UCMSCs could significantly increase the number of primordial follicles [SMD = 3.71, 95% CI [2.36, 5.07], P < 0.001], primary follicles [SMD = 3.54 , 95% CI [2.66, 4.42]], secondary follicles [SMD = 3.00 , 95% CI [1.36, 4.64], P < 0.001], and antral follicles [SMD = 3.35, 95% CI [2.28, 4.43], P < 0.001] in mice with POF , all with statistical significance. Additionally, it could significantly increase the serum estradiol concentration (E2) [SMD = 3.49 , 95% CI [1.77, 5.20], P < 0.001] and anti-Müllerian hormone (AMH) levels [SMD = 2.76, 95% CI [1.21, 4.30], P < 0.001], and significantly decrease the serum follicle-stimulating hormone (FSH) level [SMD = -2.70, 95% CI [-4.29, -1.10], P < 0.001], all with statistical significance.</p><p><strong>Conclusion: </strong>These preclinical findings indicate that transplantation of UCMSCs may improve ovarian follicle-related outcomes and hormone profiles in mouse models of POF. These results support the preclinical therapeutic potential of UCMSCs for POF. However, given the high heterogeneity and methodological limitations of included preclinical studies, these findings cannot be directly translated to clinical practice. warranting further investigation to translate these results to clinical practice.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13417847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148055113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}