{"title":"Molecular characteristics of early- and late-onset ovarian cancer: insights from multidimensional evidence.","authors":"Yanting Shen, Jie Cheng, Qing Ding, Zhihui Tao","doi":"10.1186/s13048-025-01664-9","DOIUrl":"https://doi.org/10.1186/s13048-025-01664-9","url":null,"abstract":"<p><strong>Background: </strong>Ovarian cancer (OC) is among the most lethal gynecologic malignancies, characterized by poor prognosis. While aging is a well-established risk factor, the underlying mechanisms distinguishing early- and late-onset ovarian cancer remain poorly understood.</p><p><strong>Methods: </strong>This study analyzed the global burden and age-related trends of ovarian cancer using the GBD database. A cut-off age of 55 years was used to differentiate between early and late onset ovarian cancer, and a Mendelian randomization method was also used to investigate the causal relationship between aging and ovarian cancer. Machine learning was applied to identify tumor-specific age-associated genes, followed by bioinformatics analyses and single-cell sequencing to explore the roles of these genes and immune profile alterations in ovarian cancer. Additionally, models were constructed, and drug sensitivity analyses performed to evaluate their potential as diagnostic markers or therapeutic targets.</p><p><strong>Results: </strong>Ovarian cancer incidence and mortality exhibit age-related trends, with telomere length positively associated with increased risk (OR = 1.27, 95% CI: 1.01-1.60, P = 3.90 × 10⁻<sup>2</sup>). Older patients with OC have a worse prognosis. PRKCD and UCP2 were significantly upregulated in ovarian cancer. PRKCD facilitates epithelial-mesenchymal transition (EMT), contributing to ovarian cancer progression, while UCP2 modulates ROS dynamics, influencing chemoresistance. Immune microenvironment analysis revealed differences between high- and low-expression groups, particularly in T cells, macrophages, and other immune cells. Both genes are sensitive to a varity of drugs, including dasatinib, fluvastatin, highlighting their potential as therapeutic targets.</p><p><strong>Conclusion: </strong>Aging is a significant risk factor for ovarian cancer, with PRKCD and UCP2 closely linked to its onset and progression. These genes show promise as novel biomarkers and therapeutic targets for ovarian cancer.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"83"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NCAPH promotes glucose metabolism reprogramming and cell stemness in ovarian cancer cells through the MEK/ERK/PD-L1 pathway.","authors":"Yingying Qi, Aiping Wang, Silin Chen, Wei Chen","doi":"10.1186/s13048-025-01659-6","DOIUrl":"https://doi.org/10.1186/s13048-025-01659-6","url":null,"abstract":"<p><strong>Backgrounds: </strong>Ovarian cancer is a prevalent malignant tumor that affects the female reproductive system with the characteristic of high heterogeneity. Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) has been implicated in a variety of cancers.</p><p><strong>Methods: </strong>The expression of NCAPH before and after transfection were assessed using RT-qPCR and western blot analysis. Cell stemness was evaluated through spheroid formation assay. The extracellular acidification rate (ECAR) of ovarian cancer cells was measured utilizing Seahorse Glycolysis Stress Test Assay while oxygen consumption rate (OCR) was estimated with Seahorse Mito Stress Test Assay. Lactate production and glucose consumption were quantified using corresponding assay kits. Western blot was employed to analyze the expression of stem cell markers, glycolysis- and MEK/ERK/PD-L1 signaling pathway-related proteins. In vivo, tumor size and weight were recorded, and immunohistochemical staining was used to assess MEK/ERK/PD-L1 and KI67 expression in tumor tissues from nude mice.</p><p><strong>Results: </strong>It was observed that NCAPH expression is upregulated in ovarian cancer cells. Silencing NCAPH led to repression of both stemness characteristics and glucose metabolism reprogramming. Furthermore, knockdown of NCAPH inhibited the MEK/ERK/PD-L1 signaling pathway both in vitro and in vivo, resulting in suppressed tumor growth in mouse models.</p><p><strong>Conclusion: </strong>Collectively, silencing NCAPH impedes malignant progression of ovarian cancer through modulation of the MEK/ERK/PD-L1 pathway.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"81"},"PeriodicalIF":3.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laura Danti, Karolina Lundin, Kirsi Sepponen, Dawit A Yohannes, Juha Kere, Timo Tuuri, Juha S Tapanainen
{"title":"Correction: CRISPR/Cas9-mediated activation of NR5A1 steers female human embryonic stem cell-derived bipotential gonadal-like cells towards a steroidogenic cell fate.","authors":"Laura Danti, Karolina Lundin, Kirsi Sepponen, Dawit A Yohannes, Juha Kere, Timo Tuuri, Juha S Tapanainen","doi":"10.1186/s13048-025-01655-w","DOIUrl":"https://doi.org/10.1186/s13048-025-01655-w","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"80"},"PeriodicalIF":3.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tangyi Geng, Qiao Zhou, Ying Wang, Hui Ji, Kai Ding, Zichen Zheng, Ye Yang, Junqiang Zhang, Chun Zhao, Xiufeng Ling
{"title":"Comparison of pregnancy outcomes for high morphological scoring mosaic vs. low morphological scoring euploid embryos: a retrospective cohort study.","authors":"Tangyi Geng, Qiao Zhou, Ying Wang, Hui Ji, Kai Ding, Zichen Zheng, Ye Yang, Junqiang Zhang, Chun Zhao, Xiufeng Ling","doi":"10.1186/s13048-025-01665-8","DOIUrl":"https://doi.org/10.1186/s13048-025-01665-8","url":null,"abstract":"<p><strong>Background: </strong>Mosaic embryos have been proven to be capable of resulting in live births and have become an option for embryo transfer under certain circumstances. Recent guidelines suggested that embryo morphological scoring should be taken into consideration when selecting mosaic embryos for transfer. Therefore, we introduce a hypothesis that a high morphological scoring mosaic embryo is a better choice compared to a low morphological scoring euploid embryo.</p><p><strong>Materials and methods: </strong>This retrospective cohort study included 1641 embryo transfer cycles following next-generation sequencing (NGS)-based preimplantation genetic testing for aneuploidy (PGT-A). Participants were categorized into a mosaic group (87 cycles) and an euploid group (1554 cycles) based on the PGT-A results of the transferred embryos. Statistical methods including multivariate logistic regression analysis and propensity score matching (PSM) were employed to compare the pregnancy outcomes between mosaic and euploid embryo transfer cycles.</p><p><strong>Results: </strong>Multivariate logistic regression analysis showed that the transfer of mosaic embryos was a prognosis for the reducing live birth rate (P = 0.043). Furthermore, when comparing the pregnancy outcomes of the high morphological scoring mosaic embryo transfer group with the low morphological scoring euploid embryo transfer group, no significant differences were observed (P > 0.05). Additionally, no significant differences in pregnancy outcomes were found between both the high morphological score low proportion and segmental mosaic group and the low morphological score euploid group (P > 0.05).</p><p><strong>Conclusion: </strong>Our study indicated that morphological scoring has reference value when choosing between euploid and mosaic embryo transfers. Specifically, when the morphological score of euploid embryos is poor, mosaic embryos with high morphological scores could be a viable option after comprehensive prenatal consultation.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"79"},"PeriodicalIF":3.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Curcumin and its formulations for the treatment of polycystic ovary syndrome: current insights and future prospects.","authors":"Pooja Mallya, Shaila A Lewis","doi":"10.1186/s13048-025-01660-z","DOIUrl":"https://doi.org/10.1186/s13048-025-01660-z","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is a common gynaecological complication with alarmingly high incidence of 6-20% in women of reproductive age and leads to multifaceted symptoms such as menstrual irregularities, hyperandrogenism, polycystic ovaries, and insulin resistance. Several therapeutic methods have been recommended for PCOS including lifestyle modification, insulin sensitizer (metformin), ovulation inducers (letrozole, clomiphene citrate), hormonal pills, and surgical intervention (ovarian drilling and oophorectomy); however, these treatment modalities often cause adverse effects. Currently, phytochemicals and plant extracts have been recommended for PCOS. Among these, few phytochemicals and their formulations, curcumin (CUR) (a bioactive polyphenol from Curcuma longa), has emerged as a promising complementary PCOS therapy due to its antioxidant, anti-inflammatory, insulin-sensitizing, and ovulation inducing properties. However, CUR's clinical application is hindered by poor solubility and bioavailability. In this review, we summarize and discuss various formulations of CUR and combination therapies that have demonstrated potential in treating PCOS in animal models.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"78"},"PeriodicalIF":3.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raoul Orvieto, Nira Morag, Elena Rubin, Ravit Nahum
{"title":"Defining the LH surge in natural cycle frozen-thawed embryo transfer: the role of LH, estradiol, and progesterone.","authors":"Raoul Orvieto, Nira Morag, Elena Rubin, Ravit Nahum","doi":"10.1186/s13048-025-01658-7","DOIUrl":"https://doi.org/10.1186/s13048-025-01658-7","url":null,"abstract":"<p><strong>Objective: </strong>Several replacement protocols for frozen-thawed ET (FET) exist, with no advantage of one protocol over the others. In the present retrospective and observational study we aim to evaluate the hormonal changes round the LH surge, for better determination of the LH surge and improving the NC FET outcome. We reviewed the computerized files of all consecutive women admitted to our IVF Institute, between January 1, 2023 and June 30, 2024, who underwent NC FET cycles in our IVF Institute. The elimination of bias in this selection, for the purposes of this study, was achieved by including only patients who had two consecutive hormonal blood tests and transvaginal ultrasound evaluations prior to ovulation, on two days (D- 2) before and one day before ovulation (D- 1). Data on patient demographics and infertility-treatment-related variables were collected from the files. We studied and compared several variable between patients who conceived and those who did not, including the % changes in LH (D- 1 minus D- 2/D- 2), in estradiol (D- 2 minus D- 1/D- 2) and % change in progesterone (D- 1 minus D- 2/D- 2) levels.</p><p><strong>Results: </strong>Six hundreds and sixty-eight NC FET cycles were performed during the study periods. Pregnancy was achieved in 348 patients (pregnancy rate, 52% per cycle). Figure that is not-significantly higher than our previous reported outcome, when the LH surge was defined only by the rise in LH level (46% per cycle). Patients who conceived were significantly younger, with no in-between group differences in LH, E2 and progesterone levels. Moreover, while no differences were observed in the % changes in E2, nor LH levels, the % change in progesterone levels was significantly higher in those who conceived (1.9 + 1.5 vs 1.6 + 1.4, p < 0.013), as compared to those who did not.</p><p><strong>Conclusions: </strong>Patients undergoing NC FET should be monitored by LH, estradiol and progesterone levels. We suggest that the LH surge should be determined by an increase in LH, concomitant to a drop in estradiol and a threefold increase in progesterone levels between D- 2 to D- 1. Further large prospective studies are needed to elucidate the aforementioned recommendation prior to its routine implementation.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"77"},"PeriodicalIF":3.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Granulosa cell-specific FOXJ2 overexpression induces premature ovarian insufficiency by triggering apoptosis via mitochondrial calcium overload.","authors":"Yunxia Zhang, Qiqian Wu, Furong Bai, Yanqin Hu, Bufang Xu, Yujie Tang, Jingwen Wu","doi":"10.1186/s13048-025-01651-0","DOIUrl":"https://doi.org/10.1186/s13048-025-01651-0","url":null,"abstract":"<p><strong>Background: </strong>Follicle development is a complicated biological process that produces mature oocytes, and requires nutrients, growth factors, and steroids produced by ovarian granulosa cells (GCs). High fork head box J2 (FOXJ2) expression might negatively regulate ovarian function; however, the mechanism is unclear. This study aimed to investigate the effect and mechanism of FOXJ2 overexpression in GCs on regulating follicle development and fertility.</p><p><strong>Methods: </strong>A GC-specific conditional Foxj2 knock-in mouse model (Amh-cre; Foxj2<sup>tg/tg</sup> mouse) was generated. Reproductive phenotypes were compared between Amh-cre; Foxj2<sup>tg/tg</sup> and control mice using fertility evaluation, oocyte collection, estrus cycle analysis, hormone evaluation, and ovarian follicle assessment. Then, RNA sequencing and bioinformatic analyses were used to detect the altered transcriptome of GCs collected from the Amh-cre; Foxj2<sup>tg/tg</sup> and wild-type mice. Western blotting, transmission electron microscopy, immunofluorescence staining, and flow cytometry were used to explore apoptosis and mitochondrial calcium homeostasis. Furthermore, Chromatin immunoprecipitation-PCR and dual-luciferase reporter assays were used to detect the target gene of FOXJ2. Moreover, short hairpin RNA interference was performed on primary GCs and human ovarian granulosa-like tumor (KGN) cells to explore the relationship between FOXJ2 and its target gene in apoptosis and mitochondrial calcium overload.</p><p><strong>Results: </strong>FOXJ2 overexpression in GCs led to reduced fertility, hormonal abnormalities, and follicle atresia, starting at the initiation of sexual maturity, resulting in a premature ovarian insufficiency (POI)-like phenotype. Increased apoptosis and mitochondrial calcium overload were detected in the GCs of Amh-cre; Foxj2<sup>tg/tg</sup> mice. Mcu (encoding a mitochondrial calcium uniporter) was observed to be upregulated in the GCs of the Amh-cre; Foxj2<sup>tg/tg</sup> mice and was a direct target of FOXJ2. Moreover, Mcu knockdown restored mitochondrial calcium homeostasis and reduced the apoptosis in the GCs of the Amh-cre; Foxj2<sup>tg/tg</sup> mice and in KGN cells transfected with FOXJ2-overexpression lentivirus.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"75"},"PeriodicalIF":3.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11984056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Single-cell RNA sequencing reveals the intra-tumoral heterogeneity and immune microenvironment of small cell carcinoma of the ovary, hypercalcemic type.","authors":"Yi Gao, Kewei Zheng, Haowen Tan, Mingyi Kang, Bingjian Lu, Ling Chen, Jing Xu, Chong Lu, Ranran Chai, Congjian Xu, Yu Kang","doi":"10.1186/s13048-025-01649-8","DOIUrl":"https://doi.org/10.1186/s13048-025-01649-8","url":null,"abstract":"<p><strong>Purpose: </strong>Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and lethal cancer lacking effective treatment. Its genomic mutations and tumor microenvironment need further exploration.</p><p><strong>Methods: </strong>We performed whole-exome sequencing or gene panel test to explore the SMARCA4 mutation spectrum in SCCOHT (15 samples). Single-cell RNA sequencing was conducted on one primary lesion with matched normal ovarian tissue and one recurrent lesion to investigate the intra-tumoral heterogeneity and immune microenvironment. Multiplex immunofluorescence staining validated T cell infiltration and PD-1 expression.</p><p><strong>Results: </strong>13/15 (86.7%) patients harbored SMARCA4 mutations. The loss of heterozygosity (LOH) occurred in 10/15 (66.7%) patients. Cancer cells and immune cells were observed in SCCOHT tumors. Cancer cells were further divided into seven subtypes and one from recurrent lesion exhibited the highest stemness accompanied by high expression of genes related to cell mitosis (AURKB, CHEK2, CCNB1, WEE1), DNA repair (BRCA1, RAD51) and epigenetic (EZH2, DNMT1). Immune cells mainly included macrophages and T cells. Lipid-associated tumor-associated macrophages (TAMs) was mainly in primary lesion while inflammatory cytokine-enriched TAMs in recurrent lesion. CD4<sup>+</sup>/ CD8<sup>+</sup> T cell infiltration was observed in SCCOHT tumor and a certain proportion of T cells expressed PD-1.</p><p><strong>Conclusions: </strong>SCCOHT exhibits universal SMARCA4 LOH and significant intra-tumoral heterogeneity, suggesting potential therapeutic targets, including CHEK2, CCNB1, and WEE1. Exhausted T cells and distinct TAM subsets infiltrate tumors. Targeting macrophage polarization or cytokine signaling may also be promising. These findings provide insights for developing novel therapies to improve outcomes in SCCOHT.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"76"},"PeriodicalIF":3.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphometric analysis of neoplastic cell clusters in high-grade serous ovarian cancer ascites identifies a promising prognostic factor: a retrospective study.","authors":"Benoît Thibault, Romina D'Angelo, Samy Rigal, Mélanie White-Koning, Guillaume Bataillon, Julie Guillermet-Guibert, Céline Basset","doi":"10.1186/s13048-025-01653-y","DOIUrl":"10.1186/s13048-025-01653-y","url":null,"abstract":"<p><p>High-grade serous carcinoma of the ovary is the most frequent intraperitoneal malignancy in women. It is associated with a poor prognostic outcome owing to the late appearance of clinical signs leading to a delayed diagnosis, and with resistance to platinum-based chemotherapy. One of the clinical signs is the development of ascites. The detection of neoplastic cells in ascites fluid is important as it indicates tumor progression and is associated with shorter survival. Microscopic cytospin analysis of this fluid reveals the cytological and architectural features of the neoplastic cells, allowing the pathologist to identify rapidly the malignancy and the histologic type. In association with immunocytochemistry, this process ensures a definite diagnosis and provides a specific etiology. Our objective was to provide proof-of-principle that the automatized analysis of general cytomorphological criteria, such as carcinomatous cell clustering, in malignant ascites fluid is of prognostic value in high-grade serous carcinoma. We performed a retrospective analysis of the ascites fluid of 24 advanced-stage high-grade serous ovarian cancer patients naïve of treatment. We found that the low number of neoplastic cell clusters in fluid was significantly associated with shorter overall and progression-free survival after adjusting for WHO performance status, Sugarbaker score, age and BMI. These results were independent of the peritoneal implantation of neoplastic cells. We believe this is a promising strategy to improve high-grade serous carcinoma diagnostics using a more informative but simple analysis of ascites tumor cell morphology.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"74"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}