Journal of Ovarian Research最新文献

{"title":"Identifying ovarian cortex cell subpopulations using multicolor flow cytometry.","authors":"Sophie Frontczak, Tristan Zver, Jean-Baptiste Pretalli, Oxana Blagosklonov, Clotilde Amiot, Christophe Roux, Frederic Grenouillet, Florence Scheffler","doi":"10.1186/s13048-025-01775-3","DOIUrl":"10.1186/s13048-025-01775-3","url":null,"abstract":"<p><strong>Context: </strong>Ovarian tissue autotransplantation is currently the only proven technique for reusing ovarian tissue after fertility preservation by ovarian tissue cryopreservation. However, one of its limitations relates to the quality of the grafts, both in terms of the number of surviving follicles and the quality of the stromal environment, which is essential for follicular development and graft revascularization. The aim of this study was to validate a technique for characterizing and functionally qualifying ovarian tissue in order to identify cell sub-populations of interest.</p><p><strong>Materials: </strong>Ovarian cortex strips were collected during ovarian drilling in women suffering from polycystic ovary syndrome. After fresh or frozen ovarian tissue dissociation, the resulting ovarian cells were analyzed by multicolor flow cytometry (MFC) to determine cell yield and viability after dissociation, and to identify for specific with specific antibodies.</p><p><strong>Results: </strong>Yield was significantly higher after dissociation of fresh ovarian tissue (1,59 × 10⁶ viable nucleated cells per 100 mg of ovarian cortex) compared with frozen/thawed ovarian tissue ((1,08 × 10⁶ viable nucleated cells per 100 mg of ovarian cortex) (p = 0,0195). Conversely, viability was significantly higher after dissociation of frozen/thawed ovarian tissue (84,7%) compared with fresh ovarian tissue (84,4%) (p = 0,0367). Using a panel of antibodies enabled the identification of different sub-populations that could correspond to endothelial cells or progenitors, cells with a mesenchymal profile and pericytes.</p><p><strong>Conclusion: </strong>Although further panel development is required, MFC effectively characterizes cell populations within ovarian tissue. Non-follicular cells could be evaluated as a potential prognostic factor for the recovery of ovarian function after autotransplantation but also participate in ovarian reconstruction programs.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"215"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from mesenchymal stem cells repair ovarian function by suppressing NLRP3-mediated pyroptosis in cyclophosphamide-induced premature ovarian failure.","authors":"Xiangrong Cui, Huihui Li, Xia Huang, Tingting Xue, Shu Wang, Xinyu Zhu, Xuan Jing","doi":"10.1186/s13048-025-01785-1","DOIUrl":"10.1186/s13048-025-01785-1","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) is a debilitating condition impairing fertility and health in women. Mesenchymal stem cell-derived exosomes (MSC-EVs) have emerged as a promising therapeutic option for POF due to their regenerative capabilities. This study explores the effectiveness of human umbilical cord mesenchymal stem cell-derived exosomes (HuMSCs-Exos) in counteracting NLRP3-mediated pyroptosis and restoring ovarian function in a cyclophosphamide (CTX)-induced POF model.</p><p><strong>Methods: </strong>HuMSCs-Exos were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blot for exosomal markers. A CTX-induced POF mouse model was treated with HuMSCs-Exos to assess their impact on ovarian morphology, function, and fertility. Additionally, in vitro studies on granulosa cells (GCs) evaluated the effects of HuMSCs-Exos on cell viability, apoptosis, oxidative stress, and NLRP3 inflammasome pathway components.</p><p><strong>Results: </strong>In the CTX-induced POF model, HuMSCs-Exos treatment significantly improved ovarian structure, increased follicle counts, restored estrous cycles, and enhanced fertility outcomes. Hormonal balance was also achieved, with a notable reduction in NLRP3 inflammasome activation and oxidative stress markers. In vitro, HuMSCs-Exos promoted GCs viability and reduced apoptosis and oxidative damage, further inhibiting the NLRP3 inflammasome pathway.</p><p><strong>Conclusion: </strong>HuMSCs-Exos effectively mitigate CTX-induced POF through the suppression of NLRP3-mediated pyroptosis, enhancing ovarian function and fertility. This study underscores the potential of MSC-EV-based therapies for treating POF and possibly other inflammatory and degenerative reproductive disorders.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"216"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review.","authors":"Yifan Chu, Jialiang Zhang, Luyao Wang, Jiaxin Xie, Jiayun Chen, Xinyao Hu, Juepu Zhou, Ruolin Mao, Miao Yan, Jing Yue","doi":"10.1186/s13048-025-01793-1","DOIUrl":"10.1186/s13048-025-01793-1","url":null,"abstract":"<p><p>The incidence of early-onset malignancies in reproductive-aged women is rising, necessitating effective fertility preservation strategies. Ovarian tissue cryopreservation (OTC) remains the sole option for prepubertal girls and patients requiring urgent oncologic treatment. However, post-transplant follicular attrition-driven by ischemia-reperfusion injury, oxidative stress, and aberrant primordial follicle activation-remains a major barrier. This comprehensive review elucidates the multiscale mechanisms of cryopreservation-induced follicular damage and evaluates cutting-edge strategies, including antifreeze protein-engineered cryoprotectants, angiogenesis-modulating scaffolds, and mTOR pathway inhibition. By integrating recent advances in biomaterial science and cryobiology, this study provides actionable insights to enhance OTC clinical efficacy and advance reproductive medicine.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"217"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of Withaferin A in cancer-induced muscle and cardiac wasting.","authors":"Mahavir Singh, Rakesh C Kukreja, Darini Nagarajan, Sham S Kakar","doi":"10.1186/s13048-025-01805-0","DOIUrl":"10.1186/s13048-025-01805-0","url":null,"abstract":"<p><p>Cancer-induced cachexia is a multifactorial syndrome characterized by severe skeletal muscle and cardiac atrophy, contributing significantly to patient morbidity and mortality. Among its cardiac manifestations, right ventricular (RV) dysfunction remains an underrecognized yet critical predictor of poor prognosis across diverse malignancies. Despite its clinical significance, no FDA-approved therapies currently exist to address either cancer-associated cachexia or RV dysfunction, highlighting a major unmet medical need. Our recent studies investigated Withaferin A (WFA), a steroidal lactone derived from the Withania somnifera plant, as a potential therapeutic agent to mitigate these conditions. In preclinical studies of ovarian cancer-induced cachexia, WFA not only enhanced grip strength and improved skeletal muscle morphology but also restored LV function, as evidenced by multiple echocardiographic parameters. Mechanistically, WFA attenuated key cachexia-associated pathways, including NF-κB signaling, NLRP3 inflammasome activation, and fibrotic remodeling, while promoting proteostasis and mitochondrial homeostasis. This review integrates existing literature with new insights from our translational studies to underscore WFA's potential as a dual-action therapeutic targeting both muscular and cardiac aspects of cachexia. We further examine the pathophysiological basis of cancer-associated cachexia, the utility of murine models in elucidating cardiac cachexia mechanisms, and the challenges and future opportunities for clinical translation. Collectively, we aim to present a compelling rationale for advancing WFA as a \"First-in-Class\" therapy with the potential to change the treatment paradigm for cachexia in the oncology space.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"218"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of antioxidant and anti-inflammatory mechanisms in the protective effects of vigabatrin against ovarian ischemia-reperfusion injury in a rat model.","authors":"Elif Hizal, Esra Uyar, Eda Bingul, Betul Cicek, Özlem Demir, Renad Mammadov, Cengiz Sarigul, Cebrail Gursul, Halis Suleyman","doi":"10.1186/s13048-025-01794-0","DOIUrl":"10.1186/s13048-025-01794-0","url":null,"abstract":"<p><strong>Background: </strong>Exposure of ovarian tissue to ischaemia and subsequent reperfusion can result in oxidative and inflammatory damage. A decline in adenosine triphosphate (ATP) levels has the potential to initiate pathological processes and compromise antioxidant defence mechanisms. Vigabatrin, an antiepileptic agent, has been shown to increase levels of gamma-aminobutyric acid (GABA). GABA has the potential to increase ATP levels. The present study was conducted to evaluate the preventive effects of vigabatrin concerning ovarian ischaemia-reperfusion (I/R) injury.</p><p><strong>Methods: </strong>The experiment comprised the allocation of thirty female Wistar albino rats into five groups: a sham operation group (SOG), a vigabatrin group (VIG), an ovarian I/R group (OIR), a vigabatrin + sham operation group (VISO), and a vigabatrin + ovarian I/R group (VOIR). Surgical procedures were performed under anesthesia. The VIG and VOIR were given vigabatrin (50 mg/kg, orally). Following a period of anticipation spanning an hour, the ovaries of the SOG, OIR, and VOIR rats were accessed via an abdominal incision. The incision site was sutured without the performance of an I/R procedure on the SOG ovaries. In the OIR and VOIR, the right ovaries were subjected to three hours of ischemia, followed by six days of reperfusion. Drug treatment was administered once a day using the same method for six days. Then, the rats were euthanised with 120 mg/kg ketamine (intraperitoneally). The ovarian tissues were removed. These samples were examined for oxidants, antioxidants, and proinflammatory cytokines.</p><p><strong>Results: </strong>The I/R procedure caused an increase in malondialdehyde, tumour necrosis factor alpha, interleukin 1β, and interleukin 6 levels, as well as a diminish in total glutathione, superoxide dismutase, and catalase in the ovaries, compared to the SOG (p < 0.001). Moreover, in the present study, I/R was found to result in changes in follicle numbers, as well as congestion, dilatation of the vessels, edema, and an increase in inflammatory cells (p < 0.05). In comparison with OIR, VOIR demonstrated a decline in oxidants and proinflammatory cytokines, an augmentation in antioxidants, and a reduction in histopathological damage(p < 0.05).</p><p><strong>Conclusion: </strong>It is proposed that vigabatrin may represent a novel strategy for the prevention of ovarian injury associated with I/R.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"214"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NRF2 promotes the migration of ovarian cancer cell lines by targeting TAGLN mediated epithelial-mesenchymal transition.","authors":"Huan Wang, Panpan Zhang, Qi Cheng, Lingjie Bao","doi":"10.1186/s13048-025-01804-1","DOIUrl":"10.1186/s13048-025-01804-1","url":null,"abstract":"<p><strong>Objective: </strong>Migration is one of the essential steps of cancer cell metastasis. Here we try to demonstrate the molecular crosstalk between NRF2-TAGLN pathway and ovarian cancer migration.</p><p><strong>Methods: </strong>Western blot and Real-time PCR were used to determine the expression of NRF2 and TAGLN. Pharmacological treatment and gene intervention were employed to modulate gene expression. Wound-healing and Transwell assay were used to examine the migration ability of ovarian cancer cell lines. Dual luciferase activity and chromatin immunoprecipitation assay (CHIP) were applied to verify TAGLN as a NRF2 target gene.</p><p><strong>Results: </strong>NRF2 overexpression promoted the migration of ovarian cancer cells. Our previous microarray data indicated 18 putative NRF2 target genes. Among these genes, TAGLN, binding with actin protein, has been reported to affect cytoskeletal dynamics and cell migration. A detailed analysis identified one functional antioxidant response element (ARE) in the promoter region of TAGLN, indicating TAGLN as one NRF2 target gene. Next, we explored the role of TAGLN on ovarian cancer cell migration. It showed that TAGLN overexpression promoted ovarian cancer cell migration. Conversely, knockdown of TAGLN inhibited ovarian cancer cell migration. Furthermore, transient knockdown of TAGLN using specific siRNA notably decreased cell motility in the NRF2 overexpressed ovarian cancer cells, significantly reversing the effect of NRF2 on ovarian cancer cell migration. Besides, NRF2 overexpression activated epithelial-mesenchymal transition (EMT) pathway. While, co-transfection TAGLN siRNA in NRF2 overexpressed cancer cells leaded to the upregulation of E-cadherin and the downregulation of N-cadherin, inactivating EMT pathway.</p><p><strong>Conclusions: </strong>Our study shows that NRF2 and the novel target gene TAGLN, plays a critical role in ovarian cancer cell migration via modulating cell motility and EMT. Targeting NRF2-TAGLN axis may be a new strategy to overcome metastasis and improve the ovarian cancer prognosis.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"213"},"PeriodicalIF":4.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ulinastatin protects against cisplatin-induced ovarian damage via Nrf2/Keap1 pathway.","authors":"Liping Zhao, Yishi Wu, Xiwen Zhang, Yaru Li, Yiquan Li, Zeming Fu","doi":"10.1186/s13048-025-01760-w","DOIUrl":"10.1186/s13048-025-01760-w","url":null,"abstract":"<p><p>The administration of chemotherapeutic agents such as cisplatin frequently results in ovarian damage and premature ovarian failure (POF), severely compromising fertility and quality of life in young women. This study aimed to investigate the protective effects of ulinastatin, a compound with anti-inflammatory and antioxidant properties, against cisplatin-induced ovarian injury and to elucidate the underlying mechanisms through the Nrf2/Keap1 pathway. In vivo and in vitro models were established using cisplatin-treated rats and ovarian granulosa cells. Experimental results demonstrated that ulinastatin significantly alleviated cisplatin-induced ovarian structural damage, reduced follicular atresia, and inhibited ovarian fibrosis and inflammation. Furthermore, ulinastatin restored serum hormone levels (FSH, E2, AMH), mitigated oxidative stress by reducing ROS and MDA levels while increasing SOD and GSH levels, and suppressed apoptosis by downregulating cleaved-caspase-3 and BAX while upregulating Bcl-2. Most importantly, ulinastatin activated the Nrf2/Keap1 pathway. Keap1 silencing enhanced the protective effects of ulinastatin, whereas Keap1 overexpression attenuated these effects, confirming that the Nrf2/Keap1 pathway is the key mediator of ulinastatin's protective role in the ovary. These findings underscore the dual function of ulinastatin in repairing cisplatin-induced ovarian damage and provide a promising therapeutic strategy for preserving fertility in female cancer patients undergoing chemotherapy.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"207"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of propionate metabolism-related genes to predict prognosis and immunotherapy response in ovarian cancer.","authors":"JingJing Ni, JianPing Qiu, Yan Ma","doi":"10.1186/s13048-025-01796-y","DOIUrl":"10.1186/s13048-025-01796-y","url":null,"abstract":"<p><strong>Background: </strong>Related studies have shown that propionate metabolism-related genes (PMRGs) were associated with the progress of cancers. However, the roles of PMRGs in ovarian cancer (OC) were unclear.</p><p><strong>Methods: </strong>In this study, OC-related transcriptome data and clinical information were extracted from The Cancer Genome Atlas (TCGA),Genotype-Tissue Expression (GTEx) and Gene Expression Omnibus (GEO) databases. Firstly, the differentially expressed genes (DEGs) between OC and healthy control (HC) samples were screened by differential expression analysis. Then, the differentially expressed PMRGs (DE-PMRGs) were obtained by intersecting the DEGs with PMRGs. Next, the enrichment analyses of DEGs and DE-PMRGs were conducted to investigate the functions. Moreover, the biomarkers of OC were screened and the risk score was calculated. Then, the nomogram predicting the survival of OC was constructed. Furthermore, the tumor microenvironment analyses and drug sensitivity analysis were proceeded. In addition, the transcription factor (TF)-mRNA regulatory network was constructed to reveal the potential molecular-level regulation of biomarkers. Additionally, the expression levels of biomarkers in IOSE-80, OVCA429, hey and OVCAR-8 were detected through the Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Immunohistochemistry (IHC) was performed to validate the protein expression of key biomarkers (CETP, ALDH5A1, and PTH) in ovarian cancer tissue microarrays.</p><p><strong>Results: </strong>Totals of 280 DE-PMRGs were obtained by intersecting the 9,466 DEGs and 531 PMRGs, and these genes were associated with steroid and fatty acid metabolic process. Five biomarkers (ALDH5A1, CETP, GRIA1, PTH, and TPMT) were identified, and the nomogram was constructed with risk score, age and TMB. Among them, GRIA1 was a negative factor, while age and risk score were negatively associated with patients' survival. Noticeable, the tumor purity was low and the level of immune escape was high in OC groups. Besides, AKT.inhibitor.VIII,A.443654,LFM.A13,BMS.509744 and BMS.536924 were positively associated with the risk score. Furthermore, the TF-mRNA regulatory network of OC was constructed, among them, EGR1 was the key TF which could regulate ALDH5A1 and TPMT simultaneously. The qRT-PCR proved the up-regulated expression levels of ALDH5A1, CETP, PTH and TPMT in OVCA429, hey and OVCAR-8. IHC results confirmed significantly higher protein expression of CETP, ALDH5A1, and PTH in ovarian cancer tissues compared to normal controls (p < 0.05), further validating their roles as potential prognostic biomarkers.</p><p><strong>Conclusion: </strong>This study identified 5 biomarkers associated with the prognosis of OC, which might be helpful in understanding the roles of PMRGs in the development of OC in depth. The IHC validation provided additional evidence at the protein level, reinforcing the clinical relevance of these findings.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"209"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superovulation alters telomere length and telomerase component levels in mouse oocytes.","authors":"Betul Tire, Gunel Talibova, Yesim Bilmez, Saffet Ozturk","doi":"10.1186/s13048-025-01735-x","DOIUrl":"10.1186/s13048-025-01735-x","url":null,"abstract":"<p><p>Assisted reproductive technologies (ARTs) are widely used to treat infertility and include the fundamental step, controlled ovarian stimulation (also known as superovulation). Superovulation involves the administration of gonadotropins to produce a sufficient number of oocytes, either through single or repeated applications. However, superovulation can cause certain adverse effects such as increased oxidative stress, decreased oocyte quality, and mitochondrial dysfunction. As oxidative stress and mitochondrial dysfunction are closely associated with alterations in telomere length, we investigated the effects of single and repeated superovulation on expression of the telomerase components and telomere length in mouse oocytes at germinal vesicle (GV) or metaphase II (MII) stage. Additionally, we measured serum levels of estradiol, progesterone, and oxidative markers. Our findings revealed that superovulation significantly affected telomere length, TERT protein level, telomerase RNA component (Terc), and telomerase reverse transcriptase (Tert) mRNA levels in these oocytes possibly due to altered estradiol and progesterone profiles (P < 0.05). These results suggest that altered telomerase expression and telomere length may contribute to emerging adverse effects of superovulation during oocyte maturation and early embryo development.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"210"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of efficacy, safety, and economy of recombinant and urinary follicle-stimulating hormone in women with a predicted normal response undergoing assisted reproductive technology.","authors":"Leizhen Xia, Li Cai, Lu Fan, Lifeng Tian, Houyang Chen, Leixiang Xia, Yan Zhao","doi":"10.1186/s13048-025-01800-5","DOIUrl":"10.1186/s13048-025-01800-5","url":null,"abstract":"<p><strong>Background: </strong>The administration of exogenous gonadotropins (Gn) to elicit multi-follicular development for controlled ovarian stimulation (COS) represents a pivotal component of assisted reproductive technology (ART). Presently, the exogenous Gn preparations most frequently utilized are recombinant follicle-stimulating hormone (rFSH) and urinary follicle-stimulating hormone (uFSH). Given the contentious results from previous studies, it is imperative to conduct a comprehensive assessment of their efficacy, safety, and economy.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted from January 2017 to December 2021, encompassing 3,966 women with a predicted normal response who underwent ART treatment. These patients were stratified into two groups based on the type of exogenous Gn administered: the rFSH group (N = 1,785) and the uFSH group (N = 2,181). Propensity score matching (PSM) and Cox proportional hazards model were utilized to control potential confounders. The primary evaluation indicators for efficacy, safety, and economy were the cumulative delivery rate (CDR) per initiated cycle, the incidence of moderate-to-severe ovarian hyperstimulation syndrome (OHSS), and the cost of controlled ovarian stimulation (COS), respectively. After matching all baseline characteristics, each group retained 1,133 cycles, with the baseline characteristics between the two groups being comparable.</p><p><strong>Results: </strong>Our results showed that the uFSH group exhibited a higher Gn starting dose, total Gn dose, and a longer stimulation duration compared to the rFSH group after PSM. Conversely, the rFSH group had a higher number of ≧ 14 mm follicles on trigger day, oocytes retrieved, and transferable embryos. There were no significant differences in the live birth rate for both fresh embryo transfer (50.3% vs. 51.7%) and frozen embryo transfer (44.2% vs. 42.5%) between rFSH and uFSH group. CDR were also comparable (56.1% vs. 55.0%), with an adjusted hazard ratio of 0.98 (95% confidence interval: 0.88-1.09). The incidence of moderate-to-severe OHSS was also similar in both groups (1.0% vs. 0.6%). However, the rFSH group incurred significantly higher costs of COS (RMB, 8947.6 ± 1888.0 vs. 5958.0 ± 1057.4; P < 0.001).</p><p><strong>Conclusions: </strong>In women with a predicted normal response undergoing ART treatment, rFSH and uFSH result in similar CDR and incidence of moderate-to-severe OHSS. However, the rFSH leads to a higher cost of COS.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"208"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}