Journal of Ovarian Research最新文献

{"title":"Identification of exosome-related SERPINB1 as a novel predictor for tumor immune microenvironment and clinical outcomes in ovarian cancer.","authors":"Rui Gu, Liping Jiang, Shuqin Dai, Yajie Yue, Shangjin Li, Shudan Zheng, Liwei Wu, Shaojie Zhao","doi":"10.1186/s13048-025-01589-3","DOIUrl":"https://doi.org/10.1186/s13048-025-01589-3","url":null,"abstract":"<p><strong>Background: </strong>With a high global incidence of over three million new cases in 2020 and a high mortality of over two million fatalities, ovarian cancer is one of the most common malignant tumors in gynecology. Exosomes can control the immunological condition of the tumor microenvironment (TME) by participating in intercellular interactions. Therefore, we aimed to construct an exosome-related prognostic model to predict the clinical outcomes of ovarian cancer patients.</p><p><strong>Methods: </strong>In this research, expression patterns of exosome-related genes were examined in multiple single-cell RNA-sequencing and bulk RNA-sequencing datasets. In addition, a novel exosome-related prognostic model was established by the least absolute shrinkage and selection operator (LASSO) regression method. Then, the correlations between risk score and immunological characteristics of the TME were explored. Moreover, SERPINB1, a gene in the prognostic signature, was further analyzed to reveal its value as a novel biomarker.</p><p><strong>Results: </strong>In the current study, combined with single-cell and bulk omics datasets, we constructed an exosome-related prognostic model of four genes (LGALS3BP, SAT1, SERPINB1, and SH3BGRL3). Moreover, the risk score was associated with worse overall survival (OS) in ovarian cancer patients. Further analysis found that patients with high-risk score tended to shape a desert TME with hardly infiltration of immune cells. Then, SERPINB1, positively correlated with the favorable OS and negatively with the risk score, was chosen as the representative biomarker of the model. Moreover, SERPINB1 was positively correlated with the infiltration of immune subpopulations in both public and in-house cohort. In addition, the high-resolution analysis found that SERPINB1⁺ tumor cells communicated with microenvironment cells frequently, further explaining the potential reason for shaping an inflamed TME.</p><p><strong>Conclusion: </strong>To sum up, we established a novel exosome-related prognostic model (LGALS3BP, SAT1, SERPINB1, and SH3BGRL3) to predict the prognosis of patients with ovarian cancer and identify the immunological characteristics of the TME. In addition, SERPINB1 was identified as a promising biomarker for prognostic prediction in ovarian cancer.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"65"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Shikonin reduces M2 macrophage population in ovarian cancer by repressing exosome production and the exosomal galectin 3-mediated β-catenin activation.","authors":"Min Wang, Yangyan Sun, Rui Gu, Yan Tang, Guorong Han, Shaojie Zhao","doi":"10.1186/s13048-025-01652-z","DOIUrl":"10.1186/s13048-025-01652-z","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"63"},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the quality of ovarian tissue cryopreservation by conventional slow cryopreservation and vitrification-a systematic review and meta-analysis.","authors":"Qingduo Kong, Cheng Pei, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko","doi":"10.1186/s13048-024-01561-7","DOIUrl":"10.1186/s13048-024-01561-7","url":null,"abstract":"<p><strong>Background: </strong>Ovarian tissue cryopreservation is increasingly applied in patients undergoing gonadotoxic radiotherapy or chemotherapy treatment or other patients who need to preserve their fertility. However, there is currently limited evidence to know which type of ovarian tissue cryopreservation is better. The advantages and disadvantages of conventional slow cryopreservation and vitrification are still controversial. The purpose of this meta-analysis was to analyze the ovarian tissue quality of ovarian tissue cryopreservation by conventional slow cryopreservation and vitrification.</p><p><strong>Methods: </strong>According to the keywords, Pubmed, Embase, and Cochrane Library were searched for studies to January 2024. Studies comparing the follicular viability of conventional slow cryopreservation versus vitrification were assessed for eligibility. The meta-analysis was performed using Stata software (Version 12.0) and Review Manager (Version 5.2).</p><p><strong>Results: </strong>A total of 18 studies were included in this meta-analysis. The pooled results of the primary outcomes indicated that there was no difference between the two approaches for follicular viability (RR = 0.96, 95% CI: 0.84-1.09, P = 0.520, I² = 95.8%, Random-effect), the proportion of intact primordial follicles (RR = 1.01, 95% CI: 0.94-1.09, P = 0.778, I² = 70.6%, Random-effect). The pooled results of the secondary outcomes indicated that there was no difference between the two approaches for the proportion of DNA fragmented follicles (RR = 1.20, 95% CI: 0.94-1.54, P = 0.151, I² = 0.0%, Fixed-effect), and the proportion of stromal cells (RR = 0.58, 95% CI: 0.20-1.65, P = 0.303, I² = 99.7%, Random-effect).</p><p><strong>Conclusions: </strong>Conventional slow cryopreservation and vitrification appear to provide comparable outcomes. The heterogeneity of the literature prevents us from comparing these two techniques. Further high-quality studies are needed to enhance this statement. This meta-analysis provides limited data which may help clinicians when counselling patients.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"62"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zishen Yutai pill ameliorates ovarian reserve by mediating PI3K-Akt pathway and apoptosis level in ovary.","authors":"Jianzhao Chen, Nannan Wang, Zilun Peng, Xiufei Pang, Na Ning, Qingyun Du, Qiuping Guo, Qiuling Huang","doi":"10.1186/s13048-025-01643-0","DOIUrl":"10.1186/s13048-025-01643-0","url":null,"abstract":"<p><p>Zishen Yutai Pill (ZYP) is a widely used Chinese patent medicine in the clinical treatment of diminished ovarian reserve (DOR) induced infertility in China. However, the pharmacological actions and underlying mechanisms of ZYP in treating DOR remain poorly understood. This study aimed to evaluate the therapeutic effects of ZYP on DOR and elucidate its potential mechanisms. Two models including tripterygium glycosides (TGs)-induced DOR rat model and pZP3-induced DOR mouse model were selected to assess the effectiveness of ZYP in treating DOR. The intervention lasted for 4 weeks in both models. ZYP administration significantly increased the primordial follicles and the serum levels of AMH both in DOR rat and mouse model. Further, ZYP regulated the apoptosis pathway and apoptosis-related molecules including PI3K-Akt signaling pathway, Bcl6 and Abtb2 in ovary of DOR rat. The ovarian apoptosis level was significantly downregulated in a dose-depended manner in ZYP groups. Thus, we demonstrate that ZYP improved the ovarian reserve in DOR models. The mechanisms of ZYP on DOR may be mediated through decreasing the apoptosis level by the regulation of PI3K-Akt signaling pathway and apoptosis molecules (Bcl6, Abtb2) in ovary.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"61"},"PeriodicalIF":3.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Nrf2 as a target in ovary and ovarian dysfunctions focusing on its antioxidant properties.","authors":"Taha Ghantabpour, Nasim Goudarzi, Houman Parsaei","doi":"10.1186/s13048-025-01639-w","DOIUrl":"10.1186/s13048-025-01639-w","url":null,"abstract":"<p><p>Female infertility is a common issue caused by various factors, such as hormonal imbalances, age-related decline in oocyte quality, and lifestyle choices. Ovarian dysfunction is a prevalent cause, impacting fertility by damaging cells and impairing functions. Oxidative stress (OS) is a condition resulting from an imbalance between natural antioxidants and the generation of oxidants. This phenomenon acts as a double-edged sword, playing a crucial role as a signaling mechanism in both physiological and pathological processes related to the female reproductive system. OS is linked to ovarian dysfunction, leading to cell damage and reduced fertility. Nrf2 is a key regulator in oxidative homeostasis, helping to defend against OS and improve ovarian function in women of reproductive age. Therefore, this review aims to highlight the role of Nrf2 in the female reproductive system, focusing on its antioxidant properties.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"60"},"PeriodicalIF":3.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of body mass index on pregnancy outcomes in young women with low-prognosis POSEIDON criteria after in vitro fertilization/intracytoplasmic sperm injection.","authors":"Yiting Ren, Chen Yang, Tian Tian, Rong Li, Lina Wang, Xiumei Zhen","doi":"10.1186/s13048-025-01611-8","DOIUrl":"10.1186/s13048-025-01611-8","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investeigate the pregnancy outcomes of young women with low prognosis according to the POSEIDON criteria after IVF/ICSI cycles and to explore the effect of body mass index (BMI) on pregnancy outcomes.</p><p><strong>Methods: </strong>This was a retrospective cohort study conducted in women who underwent their first IVF/ICSI cycle treatment between January 2018 and December 2020, Among them, these patients who met criteria for POSEIDON group1and 3 were further categorized into four groups according to the China body mass index(BMI) classification, we analyzed the effect of BMI on pregnancy outcomes.</p><p><strong>Results: </strong>A total of 29,354 patients were conducted first IVF/ICSI cycle between January 2018 and December 2020 in our reproductive center, 5981 women who met the criteria for POSEIDON 1 and POSEIDON 3 were further categorized into four groups according to the China body mass index(BMI) classification. There were not significant differences in the implantation rate and clinical pregnancy rate, regardless of fresh embryo transfer or frozen embryo transfer among the four groups (P > 0.05). The miscarriage rate of fresh embryo transfer was significantly higher in obese patients (P < 0.05), while the live birth rate of fresh embryo transfer and the cumulative live birth rate are significantly lower in obese patients(P < 0.05). BMI was a significant and independent predictor of the miscarriage rate of fresh embryo transfer (adjusted OR 1.111; 95% CI 1.042-1.184; p = 0.001) and the cumulative live-birth rate (adjusted OR 0.937; 95% CI 0.900-0.975; p = 0.001).</p><p><strong>Conclusions: </strong>Our study indicated that obesity negatively impacts the IVF/ICSI outcomes of young women with low prognosis, including higher miscarriage rate and lower live birth-rate and cumulative live-birth rate. In our study, we found that BMI was the best independent predictor of the miscarriage rate of fresh embryo transfer and cumulative live-birth rate of low-prognosis patients under 35 years old. Thus the best way to reduce these complications for young patients with a poor prognosis was to keep their BMI between 18.5 kg/m² and 24 kg/m².</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"59"},"PeriodicalIF":3.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MEOX2 mediates cisplatin resistance in ovarian cancer via E2F target and DNA repair pathways.","authors":"Fei Wang, Hao Zhang, Xiru Liu, Yuanyuan Dou, Hui Yang","doi":"10.1186/s13048-025-01641-2","DOIUrl":"10.1186/s13048-025-01641-2","url":null,"abstract":"<p><p>Ovarian cancer (OV) is a leading cause of cancer-related mortality among women worldwide. Despite the success of platinum-based chemotherapy in treating OV, the emergence of cisplatin resistance has significantly compromised its therapeutic efficacy. Therefore, understanding the mechanisms underlying cisplatin resistance and its molecular regulation is crucial for improving patient outcomes. This study, MEOX2 was identified as a key gene significantly associated with prognosis and cisplatin resistance in OV through bioinformatics analysis. Its expression level and biological functions were validated using online databases, tissue microarrays, and cellular experiments. The results demonstrated that high MEOX2 expression was closely associated with poor survival outcomes in OV patients, while its expression was significantly reduced in cisplatin-resistant cells. Further gene silencing experiments revealed that silencing MEOX2 markedly enhanced cisplatin resistance in resistant cells and significantly reduced cisplatin-induced early apoptosis, although it had no notable effect on cell proliferation. Moreover, the study showed that MEOX2 was not associated with immune cell infiltration in OV but was positively correlated with angiogenesis-related genes. In cisplatin-resistant cells, gene set enrichment analysis of MEOX2 co-expressed genes highlighted the activation of the E2F target and DNA repair pathway. Additionally, MEOX2 exhibited a significant negative correlation with the MCM protein family. In summary, MEOX2 is highly expressed in OV and is associated with poor patient prognosis. It may confer cisplatin resistance to OV cells by activating the E2F target and DNA repair pathway to mitigate cisplatin-induced early apoptosis. Despite certain limitations, these findings provide novel insights into the potential role of MEOX2 as a prognostic biomarker and therapeutic target in OV.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"58"},"PeriodicalIF":3.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Negligible serum anti-Müllerian hormone levels and successfully spontaneous pregnancy three times: a case report.","authors":"Yiman Fu, Baoyi Huang, Lin Ma","doi":"10.1186/s13048-025-01640-3","DOIUrl":"10.1186/s13048-025-01640-3","url":null,"abstract":"<p><strong>Background: </strong>Anti-Müllerian hormone (AMH) is a useful marker of ovarian reserve in reproductive-aged women. However, the predictive value of AMH for the occurrence of a spontaneous ongoing pregnancy has limits. We reported a patient with extreme low AMH achieved spontaneous conception three times.</p><p><strong>Case presentation: </strong>A 35-year-old woman, gravida 1, para 0, with a history of one miscarriage, presented with a one-year history of secondary infertility seeking reproductive assistance. Laboratory evaluation showed negligible (0.072 ng/mL or 0.514 pmol/L) AMH levels and ultrasound revealed reduced bilateral antral follicle count (AFC). She was diagnosed with diminished ovarian reserve (DOR) and counseled about her limited fertility prognosis. Despite recommendations for assisted reproductive techniques (ART), the patient pursued spontaneous conception. Two natural cycles and two ovulation induction cycles were conducted, utilizing recombinant follicle-stimulating hormone (FSH) with ovulation triggered by 8,000 IU of human chorionic gonadotropin (HCG). Remarkably, the patient successfully conceived three pregnancies and delivered four healthy children. In April 2022, she gave birth to a healthy boy weighing 3.17 kg via spontaneous vaginal delivery. In August 2023, she delivered another healthy boy weighing 3.80 kg via spontaneous vaginal delivery. Subsequently, in November 2024, she delivered healthy twins-one boy and one girl-via spontaneous vaginal delivery.</p><p><strong>Conclusions: </strong>This case underscores the clinical significance of specialized reproductive medicine intervention in achieving successful pregnancy outcomes in patients with rapidly declining and persistently low AMH levels. It highlights that even in cases of severely diminished ovarian reserve, natural conception is possible with expert guidance. Clinicians should exercise prudence when providing prognostic guidance regarding fertility among patients presenting with markedly diminished or undetectable AMH concentrations. The application of AMH measurement for fertility assessment in the general population still need to be clarified in well-designed prospective studies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"57"},"PeriodicalIF":3.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11916997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics reveals homogeneity and heterogeneity between physiological and pathological ovarian aging.","authors":"Lihua Zeng, Yunyi Liang, Lizhi Huang, Zu'ang Li, Manish Kumar, Xiasheng Zheng, Jing Li, Songping Luo, Ling Zhu","doi":"10.1186/s13048-025-01625-2","DOIUrl":"10.1186/s13048-025-01625-2","url":null,"abstract":"<p><strong>Background: </strong>Ovarian aging is the main cause of reduced reproductive life span, yet its metabolic profiles remain poorly understood. This study aimed to reveal the metabolic homogeneity and heterogeneity between physiological and pathological ovarian aging.</p><p><strong>Methods: </strong>Seventy serum samples from physiological ovarian aging participants, pathological ovarian aging participants (including diminished ovarian reserve (DOR), subclinical premature ovarian insufficiency (scPOI) and premature ovarian insufficiency (POI)), as well as healthy participants were collected and analyzed by untargeted metabolomics.</p><p><strong>Results: </strong>Five homogeneous differential metabolites (neopterin, menaquinone, sphingomyelin (SM) (d14:1/24:2), SM (d14:0/21:1) and SM (d17:0/25:1)) were found in both physiological and pathological ovarian aging. While five distinct metabolites, including phosphoglyceride (PC) (17:0/18:2), PC (18:2e/17:2), SM (d22:1/14:1), SM (d14:1/20:1) and 4-hydroxyretinoic acid were specific to pathological ovarian aging. Functional annotation of differential metabolites suggested that folate biosynthesis, ubiquinone and other terpenoid-quinone biosynthesis pathways, were mainly involved in the ovarian aging process. Meanwhile, dopaminergic synapses pathway was strongly associated with scPOI, vitamin digestion and absorption and retinol metabolism were associated with POI. Furthermore, testosterone sulfate, SM (d14:0/28:1), PC (18:0e/4:0) and 4-hydroxyretinoic acid, were identified as potential biomarkers for diagnosing physiological ovarian aging, DOR, scPOI, and POI, respectively. Additionally, SM (d14:1/24:2) strongly correlated with both physiological and pathological ovarian aging. 4-hydroxyretinoic acid was strongly correlated with pathological ovarian aging.</p><p><strong>Conclusions: </strong>Metabolic homogeneity of physiological and pathological ovarian aging was related to disorders of lipid, folate, ubiquinone metabolism, while metabolic heterogeneity between them was related to disorders of lipid, vitamin and retinol metabolism.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"56"},"PeriodicalIF":3.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal relationships of gut microbiota and blood metabolites with ovarian cancer and endometrial cancer: a Mendelian randomization study.","authors":"Jinyan Chen, Xuejun Chen, Jiong Ma","doi":"10.1186/s13048-025-01630-5","DOIUrl":"10.1186/s13048-025-01630-5","url":null,"abstract":"<p><strong>Objectives: </strong>The study aimed to investigate the causal relationships of gut microbiota (GM), ovarian cancer (OC), endometrial cancer (EC), and potential metabolite mediators using Mendelian randomization (MR) analysis.</p><p><strong>Methods: </strong>Bidirectional two-sample MR analysis and reverse MR analysis of GM on OC/EC were employed to determine the causal effects of GM on OC/EC and the mediating role of blood metabolites in the relationship between GM and OC/EC, with results validated through sensitivity analysis.</p><p><strong>Results: </strong>We identified 6 pathogenic bacterial taxa associated with OC, including Euryarchaeota, Escherichia-Shigella, FamilyXIIIAD3011group, Prevotella9, and two unknown genera. Christensenellaceae R.7group, Tyzzerella3, and Victivallaceae were found to be protective against OC. The increase in EC risk was positively associated with Erysipelotrichia, Erysipelotrichaceae, Erysipelotrichales, and FamilyXI. Dorea, RuminococcaceaeUCG014, and Turicibacter exhibited a negative correlation with the EC risk. A total of 26 and 19 blood metabolites related to GM were identified, showing significant correlations with OC and EC, respectively. Cytosine was found to be an intermediate metabolite greatly associated with EC and FamilyXI. In reverse MR analysis, the FamilyXIIIAD3011group exhibited a significant bidirectional causal relationship with OC.</p><p><strong>Conclusion: </strong>Our study revealed causal relationships of GM and intermediate metabolites with OC/EC, providing new avenues for understanding OC/EC and developing effective treatment strategies.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"54"},"PeriodicalIF":3.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}