Functional study of laminin subunit α5 in high-grade serous ovarian cancer.

IF 4.2 3区医学 Q1 REPRODUCTIVE BIOLOGY

Journal of Ovarian Research Pub Date : 2025-07-19 DOI:10.1186/s13048-025-01752-w

Wang Tianli, Shaojing Li, Rong Zhang

{"title":"Functional study of laminin subunit α5 in high-grade serous ovarian cancer.","authors":"Wang Tianli, Shaojing Li, Rong Zhang","doi":"10.1186/s13048-025-01752-w","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the expression of LAMA 5 (laminin subunit α5) in high-grade serous ovarian cancer and its relationship with the prognosis of ovarian cancer patients.Methods: The expression of LAMA5 in high-grade serous ovarian cancer was explored through the ovarian cancer sample information in multiple databases and real-time fluorescent quantitative PCR (Real Time Quantitative PCR, RT-qPCR). Effect of LAMA5 on the proliferation and migration of ovarian cancer cells. The possible mechanism of LAMA 5 affecting the malignant process of ovarian cancer was searched by GSEA analysis.Results: Based on bioinformatics analysis and RT-qPCR verification in multiple databases, it was found that LAMA5 was highly expressed in serous ovarian cancer, which was related to clinical stage and indicated poor prognosis. High expression of LAMA 5 can promote the proliferation and migration of ovarian cancer cells. Based on the GSEA analysis, it is suggested that the process of LAMA5 promoting the occurrence and development of ovarian cancer may be related to the mitotic pathway of the spindle (Mitotic Spindle) and the Hedgehog (Hh) signaling pathway.Conclusion: LAMA 5 may be used as a clinical marker for evaluating the prognosis of serous ovarian cancer and a new therapeutic target.","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"157"},"PeriodicalIF":4.2000,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12275312/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Ovarian Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13048-025-01752-w","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To investigate the expression of LAMA 5 (laminin subunit α5) in high-grade serous ovarian cancer and its relationship with the prognosis of ovarian cancer patients.

Methods: The expression of LAMA5 in high-grade serous ovarian cancer was explored through the ovarian cancer sample information in multiple databases and real-time fluorescent quantitative PCR (Real Time Quantitative PCR, RT-qPCR). Effect of LAMA5 on the proliferation and migration of ovarian cancer cells. The possible mechanism of LAMA 5 affecting the malignant process of ovarian cancer was searched by GSEA analysis.

Results: Based on bioinformatics analysis and RT-qPCR verification in multiple databases, it was found that LAMA5 was highly expressed in serous ovarian cancer, which was related to clinical stage and indicated poor prognosis. High expression of LAMA 5 can promote the proliferation and migration of ovarian cancer cells. Based on the GSEA analysis, it is suggested that the process of LAMA5 promoting the occurrence and development of ovarian cancer may be related to the mitotic pathway of the spindle (Mitotic Spindle) and the Hedgehog (Hh) signaling pathway.

Conclusion: LAMA 5 may be used as a clinical marker for evaluating the prognosis of serous ovarian cancer and a new therapeutic target.

Abstract Image

查看原文本刊更多论文

层粘连蛋白亚基α5在高级别浆液性卵巢癌中的功能研究。

目的：探讨lama5（层粘连蛋白亚基α5）在高级浆液性卵巢癌中的表达及其与卵巢癌患者预后的关系。方法：通过多个数据库的卵巢癌样本信息和实时荧光定量PCR (Real Time quantitative PCR, RT-qPCR)，探讨LAMA5在高级别浆液性卵巢癌中的表达情况。LAMA5对卵巢癌细胞增殖和迁移的影响。通过GSEA分析，探讨LAMA 5影响卵巢癌恶性过程的可能机制。结果：基于生物信息学分析和多个数据库的RT-qPCR验证，发现LAMA5在浆液性卵巢癌中高表达，与临床分期相关，预示预后不良。高表达的lama5可促进卵巢癌细胞的增殖和迁移。基于GSEA分析，提示LAMA5促进卵巢癌发生发展的过程可能与纺锤体（mitotic spindle）有丝分裂通路和Hedgehog （Hh）信号通路有关。结论：lama5可作为评价浆液性卵巢癌预后的临床指标和新的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Ovarian Research REPRODUCTIVE BIOLOGY-

CiteScore

6.20

自引率

2.50%

发文量

125

审稿时长

>12 weeks

期刊介绍： Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ. Topical areas include, but are not restricted to: Ovary development, hormone secretion and regulation Follicle growth and ovulation Infertility and Polycystic ovarian syndrome Regulation of pituitary and other biological functions by ovarian hormones Ovarian cancer, its prevention, diagnosis and treatment Drug development and screening Role of stem cells in ovary development and function.