cirvegfa通过与miR-21-3p结合，上调TMX4表达，抑制猪卵巢颗粒细胞凋亡。

IF 4.2 3区医学 Q1 REPRODUCTIVE BIOLOGY

Journal of Ovarian Research Pub Date : 2025-07-17 DOI:10.1186/s13048-025-01738-8

Xinxin Qin, Jinbi Zhang, Chao Yin, Fan Li, Wenjie Li, Xiaolong Cheng, Xing Du, Qifa Li, Zengxiang Pan

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引用次数: 0

摘要

背景：卵泡闭锁是多产母猪卵巢功能衰竭的主要决定因素。非编码rna （ncRNAs）在卵巢颗粒细胞（GCs）凋亡调控机制中发挥重要作用。方法：采用RNase R和放线菌素D对环状vegfa的环状结构进行验证。采用si-RNA转染法研究环vegfa在GCs细胞凋亡中的作用。此外，通过双荧光素酶报告基因测定和与它们的抑制剂或siRNA共转染，证实了cirvegfa和TMX4与miR-21-3p的竞争性结合。结果：在这项研究中，我们提出了一种新的环状RNA (circRNA)，环状vegfa，与健康卵泡（HFs）相比，它在闭锁卵泡（AFs）中的表达显著降低。结论：本研究表明，circVEGFA通过与miR-21-3p竞争结合，增加GCs中TMX4的表达，抑制细胞凋亡。本研究有助于理解卵泡闭锁后circRNA的调控。

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circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression.

Background: Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs).

Methods: The circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA.

Results: In this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs).

Conclusions: The study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia.

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来源期刊

Journal of Ovarian Research REPRODUCTIVE BIOLOGY-

CiteScore

6.20

自引率

2.50%

发文量

125

审稿时长

>12 weeks

期刊介绍： Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ. Topical areas include, but are not restricted to: Ovary development, hormone secretion and regulation Follicle growth and ovulation Infertility and Polycystic ovarian syndrome Regulation of pituitary and other biological functions by ovarian hormones Ovarian cancer, its prevention, diagnosis and treatment Drug development and screening Role of stem cells in ovary development and function.