Journal of Oral Pathology & Medicine最新文献

{"title":"Multifocal Perineural Invasion Predicts Poor Disease-Free Survival in Early-Stage Tongue Squamous Cell Carcinoma Exhibiting WPOI-4","authors":"Xiaoli Jia,&nbsp;Ting Gu,&nbsp;Jiang Li","doi":"10.1111/jop.70133","DOIUrl":"10.1111/jop.70133","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Perineural invasion (PNI) is a pathological hallmark associated with tumor spread. Evidence suggested that PNI location (extratumoural vs. intratumoural) held prognostic significance in early-stage oral squamous cell carcinoma (OSCC), whereas the prognostic impact of PNI extent (unifocal versus multifocal) remained to be elucidated. The primary aim of this study was to evaluate the prognostic relevance of PNI extent in early-stage tongue squamous cell carcinoma (TSCC) characterized by worst pattern of invasion 4 (WPOI-4).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 130 patients with pT1/T2 pN0/Nx TSCC were included in this retrospective study, of whom 104 did not receive postoperative radiotherapy (PORT), while the remaining 26 underwent PORT. Based on the Yamauchi–Kuratomi (YK) classification, WPOI-4 cases were further stratified into 4C/4D and non-4C/4D subtypes. Depth of invasion (DOI) was dichotomized using a 4 mm threshold.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Multivariate analysis revealed that both multifocal and unifocal PNI were significantly associated with disease-free survival (DFS), whereas no meaningful association was observed with overall survival (OS). Compared to unifocal PNI (odds ratio [OR] = 5.504; 95% confidence interval (CI) = 1.994–15.188; <i>p</i> &lt; 0.001), multifocal PNI conferred an approximately fivefold increased risk of disease progression (OR = 27.009; 95% CI = 8.695–83.900; <i>p</i> &lt; 0.001). In addition, multifocal PNI showed a notable correlation with DOI &gt; 4 mm and 4C/4D histology. Elective neck dissection (END) combined with postoperative radiotherapy (PORT) significantly reduced disease progression in PNI-positive patients, particularly those with multifocal PNI (OR = 0.01; 95% CI = 0.002–0.051; <i>p</i> &lt; 0.001). Among patients who did not receive PORT, END showed limited benefit in multivariate analysis. However, after excluding cases with isolated local recurrence, END was associated with improved DFS in patients with cervical lymph node metastasis (CLNM) (<i>p</i> = 0.017).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings support incorporating the extent of PNI into routine pathology reporting and suggest that END and PORT may be considered, particularly in cases of multifocal PNI.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"695-704"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"p16 and MGMT Methylation in Chronic Traumatic Ulcers: A Plausible Link Between Chronic Mechanical Irritation and Oral Carcinogenesis?","authors":"Jeronimo Pablo Lazos,&nbsp;Marcela Hernandez Rios,&nbsp;Jessica Astorga,&nbsp;Rene Luis Panico,&nbsp;Eduardo David Piemonte,&nbsp;Ana María Zárate","doi":"10.1111/jop.70128","DOIUrl":"10.1111/jop.70128","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chronic mechanical irritation (CMI) has been proposed as a risk factor for oral cancer. Epigenetic alterations, particularly methylation, are early events in carcinogenesis, and it has been proposed that they can be prompted by CMI. Thus, the aim of this study was to describe p16 and MGMT methylation in chronic traumatic ulcer (CTU).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A case–control split-mouth study was performed. Two samples per individual (<i>N</i> = 27) were taken using exfoliative cytology using a split-mouth design, one from the CTU and the other from a contralateral site of clinically normal mucosa. DNA was extracted and bisulfite-treated, and specific sites at the promoter region of p16 and MGMT genes were amplified by qPCR using validated primers. Then, the PCR product was sequenced. The statistical analysis was performed by McNemar and the Chi-square test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Patients had a mean age of 59.1 years. CTU showed higher methylation than control sites for both p16 (85% vs. 20%, <i>p</i> &lt; 0.0001) and MGMT (80% vs. 24%, <i>p</i> &lt; 0.0005).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Oral mucosa subjected to continuous exposure to CMI is associated with increased methylation of p16 and MGMT. Proper management of mechanical injury factors could be an important measure for OSCC prevention. In CMI, exfoliative cytology and the split-mouth design could be useful tools to study biomarkers. However, the role of CMI in oral carcinogenesis needs more evidence focusing on the biological phenomenon.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"669-675"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial-Mesenchymal Transition, Immune Response, and Extracellular Matrix Remodelling in Oral Cancer","authors":"Nicole Lonni,&nbsp;Camilla Kammer Pereira,&nbsp;Amanda Maciel Costa,&nbsp;Aline Hsiao Bona,&nbsp;Andressa Fernanda Paza Miguel,&nbsp;Elis Ângela Batistella,&nbsp;Daniella Serafin Couto Vieira,&nbsp;Elena Riet Correa Rivero","doi":"10.1111/jop.70126","DOIUrl":"10.1111/jop.70126","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This retrospective study investigates the interplay between epithelial-mesenchymal transition (EMT) and immune response within the tumor microenvironment (TME) of oral squamous cell carcinoma (OSCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Immunohistochemistry was conducted on OSCC specimens to evaluate the expression of podoplanin (PDP), E-cadherin (CDH1), vimentin (VIM), fibronectin (FN), tenascin-C (TNC), and vascular endothelial growth factor (VEGF). Cancer-associated fibroblasts (CAFs) were identified by alpha-smooth muscle actin (α-SMA), and immune cells were quantified using CD66b for neutrophils and CD8 for T lymphocytes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Higher α-SMA and moderate-to-strong VEGF expression were associated with reduced CDH1 and increased VIM and PDP expression (<i>p</i> &lt; 0.05), indicating an EMT-like phenotype. Cases with elevated PDP—also linked to high α-SMA—showed increased CD66b⁺ cell density (<i>p</i> = 0.072). VEGF expression additionally correlated with greater tumor thickness and depth of invasion (<i>p</i> = 0.059). Strong TNC expression was associated with reduced CD8⁺ T-cell infiltration in the tumor centre and increased CD66b⁺ neutrophils (<i>p</i> &lt; 0.046). Strong FN expression was linked to a higher neutrophil-to-lymphocyte ratio (<i>p</i> = 0.031). Elevated CD66b⁺ cell counts and a higher neutrophil-to-lymphocyte ratio were both significantly associated with poorer overall survival (<i>p</i> &lt; 0.015).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These exploratory findings suggest that CAFs serve not only as a marker of stromal activation but may also contribute to immunomodulation and invasive tumor phenotype.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"660-668"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interpreting Machine Learning in Rare Outcomes","authors":"Carlos M. Ardila,&nbsp;Anny Marcela Vivares-Builes,&nbsp;Eliana Pineda-Vélez","doi":"10.1111/jop.70134","DOIUrl":"10.1111/jop.70134","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"617-618"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147503553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sclerosing Odontogenic Carcinoma: A Review of a Challenging Entity in Malignant Odontogenic Classification With a Focus on Perineural Invasion and Cellular Proliferation","authors":"Merva Soluk-Tekkesin,&nbsp;Keith David Hunter,&nbsp;Ronell Bologna-Molina,&nbsp;Kelly Magliocca,&nbsp;Liam Robinson,&nbsp;Akinyele Olumuyiwa Adisa,&nbsp;Jiang Li,&nbsp;Elizabeth Ann Bilodeau,&nbsp;Haizal Mohd Hussaini,&nbsp;Madhu Shrestha,&nbsp;Ricardo Santiago Gomez,&nbsp;Willie van Heerden,&nbsp;Wanninayake Mudiyanselage Tilakaratne","doi":"10.1111/jop.70125","DOIUrl":"10.1111/jop.70125","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sclerosing odontogenic carcinoma (SOC) is a rare intraosseous neoplasm defined by the World Health Organization as a locally aggressive tumor with “no metastatic potential”.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This review provides a critical evaluation of the biological behavior, clinicopathologic features, and clinical management of SOC cases, with a particular focus on neural invasion and cellular proliferation scores.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A comprehensive literature was performed using the Pubmed and Scopus databases to identify all published English-language reports of SOC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis of 22 reported cases revealed that SOC predominantly affects the mandible in middle-aged adults, typically presenting as a radiolucent lesion associated with cortical resorption. Histopathologically, perineural invasion was identified in 77.3% of cases and revealed a wide range of Ki-67 expression (1%–%30).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This challenging entity exhibits a spectrum of malignant potential, and the current classification standard of “no metastatic potential” requires revision. In cases exhibiting diverse biological behaviors, the Ki-67 proliferation score can be used an ancillary marker for risk stratification; higher rates (e.g., &gt; 10%–15%) may be associated with aggressive local invasion and metastatic events. Further molecular studies on aggressive “metastatic” cases are imperative to allow for identification of definitive markers that can establish SOC as a distinct entity and provide a rational basis for risk assessment and treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"619-628"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D Modulates Doxorubicin-Induced ACE2 Expression and Pro-Inflammatory Cytokines in the Rat Tongue","authors":"Mustafa Sami Demirsoy,&nbsp;Aras Erdil,&nbsp;Sefa Çolak,&nbsp;Ali Tuğrul Akin,&nbsp;Necla Değer,&nbsp;Derya Karabulut,&nbsp;Fadime Demir,&nbsp;Hatice Aygün","doi":"10.1111/jop.70127","DOIUrl":"10.1111/jop.70127","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>Doxorubicin (DOX) is a widely used chemotherapy drug, but its severe toxic effects, including inflammation and taste disturbances, limit its clinical use. This study examined the protective effects of vitamin D on DOX-induced changes in tongue tissue and systemic inflammation in rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Twenty-eight adult male Wistar Albino rats (10–12 weeks old) were divided into four groups: Control, DOX, Vitamin D 5000 + DOX, and Vitamin D 60 000 + DOX. Vitamin D3 was administered intraperitoneally either daily (5000 IU/kg) or 3 days a week (60 000 IU/kg) for 21 days. DOX (18 mg/kg, intraperitoneally) was administered on days 19–21. Tongue tissues were analyzed for angiotensin-converting enzyme 2 (ACE2) expression via immunohistochemistry, and serum cytokine levels (TNF-α, IL-1β, and IL-6) were measured using enzyme-linked immunosorbent assay (ELISA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>DOX treatment significantly increased ACE2 expression in tongue tissue compared with controls (<i>p</i> &lt; 0.001), accompanied by elevated serum inflammatory cytokine levels and reduced body weight. Vitamin D supplementation significantly attenuated DOX-induced ACE2 upregulation and inflammatory cytokine elevations (<i>p</i> &lt; 0.05). However, no clear dose-dependent difference was observed between the two vitamin D regimens, as ACE2 expression did not differ significantly between the 5000 IU/kg and 60 000 IU/kg groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>DOX administration is associated with increased ACE2 expression in tongue tissue and systemic inflammation in a rat model. Vitamin D supplementation mitigates these DOX-induced alterations; however, within the tested dose range, no additional benefit of the higher vitamin D dose was demonstrated. These findings suggest a potential modulatory role of vitamin D on chemotherapy-associated inflammatory and molecular changes, while highlighting the need for further mechanistic and functional studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"650-659"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological Biomarkers in Oral Leukoplakia According to Oral Intraepithelial Neoplasia (OIN) Classification: A Comparative Analysis of Salivary and Plasma Cytokines","authors":"Hakan Yülek,&nbsp;Gaye Keser,&nbsp;Başak Aru,&nbsp;Filiz Namdar Pekiner,&nbsp;Gülderen Yanıkkaya Demirel","doi":"10.1111/jop.70131","DOIUrl":"10.1111/jop.70131","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Oral leukoplakia (OL) is the most common oral potentially malignant disorder, defined as a white patch or plaque not attributable to other causes. While OL carries an inherent risk of malignant transformation, the underlying immunological changes remain poorly characterized. This study aimed to profile a broad panel of cytokines in saliva and plasma of OL patients in comparison to healthy controls, in order to elucidate local and systemic immunoinflammatory alterations associated with OL.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Thirty patients with clinically and histopathologically confirmed OL and 30 age- and gender-matched healthy controls were enrolled. Detailed clinical examinations were performed, including lesion characterization and psychosocial assessments. Unstimulated whole saliva and peripheral blood samples were collected, and concentrations of 13 cytokines/chemokines (IL-1β, IL-6, IL-8/CXCL8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33, TNF-α, IFN-γ, IFN-α2, and MCP-1/CCL2) were measured using a multiplex bead-based immunoassay. Group comparisons were performed using appropriate statistical tests (&lt;i&gt;χ&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt;/Fisher's exact test for categorical variables and &lt;i&gt;t&lt;/i&gt;-test or Mann–Whitney &lt;i&gt;U&lt;/i&gt; test for continuous variables), with statistical significance set at &lt;i&gt;p&lt;/i&gt; &lt; 0.05.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;OL patients (mean age 52.4 ± 10.7 years) and controls (59.9 ± 12.8 years) showed no significant differences in gender, socioeconomic status, or psychological scores. Salivary levels of IL-1β, TNF-α, MCP-1, IL-8, and IL-18 were significantly lower in OL patients compared to controls (&lt;i&gt;p&lt;/i&gt; &lt; 0.01 for all), whereas other salivary cytokines (IFN-α2, IFN-γ, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-33) showed no significant differences. In contrast, plasma levels of IFN-α2, IL-6, IL-12p70, IL-17A, IL-18, and IL-33 were significantly higher in OL patients than in controls (&lt;i&gt;p&lt;/i&gt; &lt; 0.05), indicating systemic inflammation, while plasma IL-1β, IFN-γ, TNF-α, MCP-1, IL-8, IL-10, and IL-23 did not differ. No significant differences in salivary cortisol, liver enzymes, or hepatitis viral markers were observed between groups. Across histopathological subgroups, cytokine concentrations did not significantly differ between lesions graded as hyperkeratosis, OIN1, OIN2, or OIN3, although a trend toward higher plasma IL-6, IL-17A, and IL-18 levels was observed in cases with higher OIN grades.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;These findings shed light on the inflammatory profile of OL. The decrease in salivary IL-1β, IL-8, TNF-α,","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"682-694"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucosal Melanoma of Head and Neck: A Rare and Aggressive Disease—A Register-Based Cohort Study","authors":"Sara C. Degerholm,&nbsp;Tuula A. Salo,&nbsp;Matti O. Mauramo","doi":"10.1111/jop.70129","DOIUrl":"10.1111/jop.70129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Mucosal melanomas of the head and neck are rare and often diagnosed in the advanced stage. This study examines the incidence, survival and histology of mucosal melanoma compared to its cutaneous counterpart.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This register-based cohort study reviews head and neck melanomas at Helsinki University Hospital, Finland, from 2002 to 2023.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 56 patients with mucosal and 714 with cutaneous melanoma were reviewed. The incidence of head and neck mucosal melanoma in the region was 1.55/million/year. The most common sites for mucosal melanomas were the nose and paranasal sinuses. Mucosal melanoma was more common in women, whereas cutaneous melanoma was more common in men (<i>p</i> &gt; 0.05). A significant difference emerged in mortality between mucosal and cutaneous melanoma, as 5-year survival was only 12.0% in the mucosal melanoma group compared with 60.3% in the cutaneous melanoma group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Mucosal melanoma is a highly aggressive malignancy with poor survival; thus, prompt diagnosis is essential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"676-681"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Expression and Potential Role of MicroRNAs in Oral Lichen Planus","authors":"Mousa Ali Heba,&nbsp;Resteu Anastasia,&nbsp;Werner Andreas,&nbsp;Carrozzo Marco","doi":"10.1111/jop.70122","DOIUrl":"10.1111/jop.70122","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral lichen planus (OLP) is a chronic T-cell-mediated immune disease of unknown aetiology. MicroRNA (miRNAs) are short non-coding RNAs capable of regulating mRNA and may have roles in T-cell-related diseases. The aim of this study was to investigate the profile of miRNAs in OLP patients and its interaction with potential target genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Total RNA was extracted from fresh frozen biopsies from 24 patients with OLP and 8 control patients. The NanoString Counter Analysis System was used to analyse total RNA samples for differential miRNA expression. NanoString expression was confirmed by RT-qPCR analysis. Genes potentially targeted by upregulated and downregulated miRNAs were identified, and RT-qPCR was employed to investigate the expression of target genes in OLP and controls. Probability values &lt; 0.05 were considered statistically significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NanoString analysis showed that eight miRNAs, miR-155, miR-146a, miR-3195, miR-342, miR-4516, miR-21, miR-29a and miR-193 were upregulated in OLP tissues. Contrarily, the other eight miRNAs, miR-221, miR-200b, miR-149, miR-205, miR-27b, miR-95, miR-127b, miR-95, miR-206 were downregulated in OLP tissues. NanoString findings have been confirmed by RT-qPCR results for four upregulated miRNAs, miR-155, miR-146a, miR-29a and miR-342, and one downregulated miRNA, namely miR-205. The expression of two target genes, namely MYC for miR-29a and interleukin-24 (IL-24) for miR-205, was found to negatively correlate with the respective miRNA. This suggests that MYC and IL-24 could be regulated by the above miRNAs in OLP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The work presented in this study suggests that miRNAs could be involved in both the immunopathogenesis and malignant transformation of OLP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"642-649"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146113477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Beneficial Effects of Topical l-Glutamine on Oral Mucositis in 5-Fluorouracil-Treated Mice","authors":"Juliana Francisca Grossi Heleno,&nbsp;Igor de Campos Fontes,&nbsp;Leticia Cristine Cardoso dos Santos,&nbsp;Victor Prokop Gonçalves Campos,&nbsp;Iury Berckmann Freitas Gomes,&nbsp;Derek William Gilroy,&nbsp;Silvia Passos Andrade,&nbsp;Paula Peixoto Campos","doi":"10.1111/jop.70135","DOIUrl":"10.1111/jop.70135","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral mucositis is a severe health and economic problem that occurs in about 40%–90% of patients undergoing cancer treatments. Systemic or topical application of <span>l</span>-glutamine has been shown to improve mucosal healing and ameliorate oral mucositis (OM) symptoms according to clinical score systems. To further establish the beneficial effects of this amino acid, our study examined the actions of topical application of <span>l</span>-glutamine on inflammation and mucosal healing markers in tongue lesions in mice treated with 5-Fluorouracil.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The animals received 3 doses of the chemotherapeutic agent (non-consecutive days) at concentrations of 60, 40, and 40 mg/kg, respectively. Tongue lesions were made by acetic acid (bolus injection) on the ventral face. Ten mg/50 μL of <span>l</span>-glutamine were applied at the site of the lesion in the treated group (<i>n</i> = 15) twice a day for 5 days; the control group (<i>n</i> = 15) received filtered water. On day 6, the animals were euthanized, the tongues removed, and processed for inflammatory and fibrogenic markers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The treatment decreased myeloperoxidase (MPO) and <i>N</i>-acetyl-<i>β</i>-glucosaminidase (NAG) activities, tumor necrosis factor alpha levels (TNF-α), and mast cell numbers at the site of the lesions. The amino acid was able to decrease the number of Ki-67 positive cells in the treated group. There was increased mucosal remodeling through collagen deposition in the treated group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Topical application of <span>l</span>-glutamine acted on multiple axes of OM, modulating inflammation and tissue remodeling in immunosuppressed mice.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 6","pages":"705-717"},"PeriodicalIF":2.3,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}