Journal of Oral Pathology & Medicine最新文献

{"title":"Tumor Cells-Derived FGF-2 Promotes Lymphangiogenesis as a Prognostic Marker in OSCC","authors":"Jia Kang,&nbsp;Aoming Cheng,&nbsp;Guanzheng Chen,&nbsp;Lin Zhu,&nbsp;Zhengxue Han,&nbsp;Qiaoshi Xu","doi":"10.1111/jop.70019","DOIUrl":"10.1111/jop.70019","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The “cold” tumor microenvironment of oral squamous cell carcinoma (OSCC), where tumor-associated lymphatic vessels play a critical role in the transport of immune cells, is associated with a poor prognosis. However, the effect of tumor-induced lymphangiogenesis on CD8+ T cell infiltration and its role in the formation of the tumor immune microenvironment remain unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We analyzed the prognostic significance of several lymphangiogenesis factors in OSCC with The Cancer Genome Atlas dataset, and confirmed the impact of fibroblast growth factor-2 (FGF-2) on prognosis in tissue specimens. Subsequently, we investigated the effects of FGF-2 on the proliferation, migration, and tube formation capacities of lymphatic endothelial cells, and CD8+ T cell infiltration through in vivo and in vitro experiments. Survival analysis was performed by Kaplan–Meier analysis and log-rank tests. The hazard ratio was calculated by Cox proportional hazards model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Patients with high FGF-2 levels and increased numbers of peritumoral lymphatic vessels were associated with a worse prognosis. Furthermore, we demonstrated that tumor cell-derived FGF-2 promoted lymphangiogenesis by regulating the FGFR1/PTEN/AKT axis and increased the secretion of CXCL9 to recruit and egress CD8+ T cells via neo-lymphatic vessels. PD-166866, an inhibitor of FGFR1, suppressed lymphangiogenesis and the secretion of CXCL9 to increase CD8+ T cell infiltration and inhibit tumor progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our data suggest that FGF-2 is a significant prognostic factor that induces lymphangiogenesis and affects intratumoral CD8+ T cells, contributing to the formation of a “cold” tumor microenvironment in OSCC. FGF-2/FGFR1 could serve as an effective target for improving the prognosis of OSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"694-705"},"PeriodicalIF":2.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daughter Cells Budding From PGCCs and Their Clinicopathological Significances in Oral Squamous Cell Carcinoma","authors":"Shanshan Zhuo,&nbsp;Chao Jing,&nbsp;Xiaonan Liu,&nbsp;Jiuling Yue,&nbsp;Wenchao Zhang,&nbsp;Shiwu Zhang","doi":"10.1111/jop.70014","DOIUrl":"10.1111/jop.70014","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Polyploid giant cancer cells (PGCCs) have emerged as a focal point in tumorigenesis and progression research. Present across various tumor tissues, PGCCs are considered a potential target for anti-tumor strategies. Nonetheless, their presence and role in oral squamous cell carcinoma (OSCC) remain undocumented. This study investigated the presence and count of PGCCs in OSCC tissues, evaluated their association with clinicopathological factors, and preliminarily examined the mechanisms underlying their formation alongside their influence on OSCC proliferation, drug resistance, and migratory capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The correlation between PGCC counts and clinical outcomes in OSCC was evaluated. CoCl₂ treatment was used to induce PGCC formation in OSCCUM1 cells, and alterations in HIF-1α, SOX-2, E-cadherin, and N-cadherin expression, along with changes in cellular proliferation, drug resistance, and migratory capacity, were assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 76 OSCC patients, PGCC counts were linked to clinical stage, histological grade, and chemotherapy response. Patients with &gt; 3 PGCCs/HP exhibited significantly poorer overall and disease-free survival compared to those with ≤ 3 PGCCs/HP. Following the induction of polyploid giant cancer cells with budding cells, miRNA analysis revealed upregulated SOX-2 expression, while Western blot demonstrated increased protein levels of HIF-1α, SOX-2, and N-cadherin, accompanied by reduced E-cadherin expression. Induced PGCCs with budding cells also exhibited enhanced proliferation, resistance to chemotherapy, and migratory capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Hypoxic conditions may promote OSCC proliferation, chemoresistance, and migration through the formation of PGCCs with budding cells characterized by epithelial-mesenchymal transition and stemness phenotypes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"687-693"},"PeriodicalIF":2.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inverse Regulation of TLR4 and PD-L1 Shapes the Inflammatory Tumor Microenvironment in Oral Squamous Cell Carcinomas","authors":"Camila Alves Ferri,&nbsp;Giuseppe Pannone,&nbsp;Maria Carmela Pedicillo,&nbsp;Giorgio Mori,&nbsp;Ilenia Sara De Stefano,&nbsp;Francesco Angelillis,&nbsp;Raffaele Barile,&nbsp;Roberta Seccia,&nbsp;Silvana Papagerakis,&nbsp;Angela Santoro,&nbsp;Gian Franco Zannoni,&nbsp;Gabriella Aquino,&nbsp;Margherita Cerrone,&nbsp;Monica Cantile,&nbsp;Vito Rodolico,&nbsp;Giuseppina Campisi,&nbsp;Renato Franco,&nbsp;Francesco Miele,&nbsp;Rosanna Zamparese,&nbsp;Francesco Longo,&nbsp;Lorenzo Lo Muzio,&nbsp;Fernanda Visioli","doi":"10.1111/jop.70012","DOIUrl":"10.1111/jop.70012","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The interactions between malignant cells and immune cells within the tumor microenvironment (TME) significantly influence cancer development and progression. This study aimed to analyze and correlate the expression of TLR4 and PD-L1 with the immune response, clinical characteristics, and prognosis of oral squamous cell carcinomas (OSCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Our retrospective multicentric study consisted of the assessment of 166 OSCC specimens for TLR4, PD-L1, CD8, and Ki-67 expression in a TMA-based immunohistochemistry analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our findings indicated an inverse correlation between the expression of PD-L1 and TLR4 (r = −0.348, <i>p</i> = 0.014, and r = −0.269, <i>p</i> = 0.049, superficial tumor site and in overall analysis, respectively). On the other hand, PD-L1 expression in the deep and superficial invasive front positively correlated with CD8+ T tumor infiltrating lymphocytes (TIL) in a statistically significant manner. A logistic regression analysis was performed to assess the impact of each variable on the clinical outcome with at least 5-year follow-up after the initial OSCC diagnosis. The multivariate model revealed that advanced T stage (T3-T4), presence of lymph node metastasis (N+), as well as performing chemotherapy were statistically significantly associated with OSCC mortality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings taken together suggest that there is a differential regulation of the immune response coordinated by activation of PD-L1 or TLR4 affecting T cell response.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"676-686"},"PeriodicalIF":2.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of N-Acetylcysteine and l-Carnitine on Wound Healing of Palatal Mucosa in a Rat Model","authors":"Gizem Güvenç,&nbsp;Aslı Erdoğan-Öner,&nbsp;Gülten Kavak,&nbsp;Selen Akyol Bahçeci,&nbsp;Onur Kutlu","doi":"10.1111/jop.70018","DOIUrl":"10.1111/jop.70018","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Surgical procedures in oral and maxillofacial surgery inevitably cause wound formation, making the patient vulnerable to infections as well as discomfort. As antioxidant agents exert great potential to accelerate wound healing, we investigated the effects of <i>N</i>-acetylcysteine (NAC) and <span>l</span>-carnitine (LC) on palatal wound healing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Sixty-four Wistar rats were randomly divided into four groups. 5 mm diameter wounds were created in the hard palates of the rats. 150 mg/kg/day NAC, 100 mg/kg/day LC, or both were injected intraperitoneally to the treatment groups until sacrification on Day 5 or 10. Hematoxylin and eosin, Masson's Trichrome staining, and immunohistochemistry (α-SMA, FGF-2) were performed on wound tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>On Days 5 and 10, reepithelialization was incomplete and inflammation was observed in all groups. Collagen density tended to increase in the LC and NAC + LC groups on Day 5, and significantly differed between the LC and NAC groups on day 10. A slight elevation in α-SMA expression was observed in the NAC group on Day 5, while NAC + LC treatment significantly reduced α-SMA levels compared to NAC alone. FGF-2 expression showed an increasing trend in the NAC + LC group on Day 5 and in the NAC and LC groups on Day 10. A significant increase in FGF-2 was observed in the NAC + LC group versus the NAC group on Day 5, and within the NAC group between Days 5 and 10.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings have shown that LC in the late stage and NAC + LC combination in the early stage may positively affect palatal wound healing.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"667-675"},"PeriodicalIF":2.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Link Between Integrins and Oral Diseases","authors":"Ersha Liu,&nbsp;Liujun Zeng","doi":"10.1111/jop.70017","DOIUrl":"10.1111/jop.70017","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Integrins are a class of membrane protein that exists widely on the cell surface. Their primary function is to mediate connections between the extracellular matrix and cytoskeleton. Integrin plays vital roles in numerous physiological and pathological processes, including the occurrence and development of diseases such as tumors and inflammation. Within the field of stomatology, integrins are also actively studied molecules. Research indicates that specific integrins play essential roles in oral diseases including oral mucositis, periodontitis, and oral cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This review involves a comprehensive search of the Web of Science and PubMed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This review synthesizes current research on integrins in oral squamous cell carcinoma, oral submucous fibrosis, oral pemphigoid, oral mucositis, periodontal disease, and peri-implantitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This review provides a systematic summary of the roles of integrins in oral biology and disease pathogenesis, helping to reduce barriers in integrin research and offering some theoretical basis for the prevention and treatment of oral diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"628-634"},"PeriodicalIF":2.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jaw Solitary Plasmacytoma of Bone and Oral Extramedullary Plasmacytoma: Literature Review","authors":"Lama Alabdulaaly,&nbsp;Herve Sroussi,&nbsp;Alessandro Villa","doi":"10.1111/jop.70011","DOIUrl":"10.1111/jop.70011","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Plasmacytoma is the second most common hematolymphoid neoplasm in the oral cavity. It can present in the bone as solitary bone plasmacytoma (SBP) or in the soft tissues as extramedullary plasmacytoma (EMP). Our goal was to summarize the reported cases of SBP affecting jaw bones and EMP affecting the oral soft tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We searched the literature on PubMed, Ovid, Embase, Web of Science, and Cochrane databases and included jaw (gnathic) SBP and oral EMP publications from January 2000 to May 2024.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We identified 161 cases of jaw SBP and oral EMP. The median age was 58 years, with 54.7% males. Male predilection was seen in oral EMP (87.5%) but not jaw SBP (48.9%). The median lesion duration was shorter for oral EMP (3 months) compared to jaw SBP (6 months). Oral EMP was more likely to be asymptomatic than jaw SBP (47.1% vs. 19.4%, respectively). The most common location for jaw SBP was the mandible (70.8%) and the gingiva and tonsils were the oral sites mostly affected by oral EMP (29.2% each). One-third of cases received radiation therapy. Remission was seen in 73.3% of oral EMP cases and 35.4% of jaw SBP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>It is important for oral health providers to be aware of the clinical presentations of jaw SBP and oral EMP. Jaw SBP and oral EMP differed clinically and prognostically based on the reported cases. This underscores the importance of correct diagnosis and differentiating the two entities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"621-627"},"PeriodicalIF":2.3,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Research Trends on the Links Between the Oral Microbiome and Cancer From 2014 to 2024: A Visualization Analysis","authors":"Umar Pervaiz,&nbsp;Pervaiz Nabeel,&nbsp;Rui Zhao,&nbsp;Zhengbin Zhao,&nbsp;Yibao Zhang,&nbsp;Degui Wang","doi":"10.1111/jop.70000","DOIUrl":"10.1111/jop.70000","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>In recent years, noteworthy connections have been discovered between human wellness and microbiota. This study aims to outline the hotspots and trends in the links between the oral microbiome and cancer over the past 10 years from a bibliometric perspective.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Reports on original research and literature reviews on the relationship between the oral microbiome and cancer from 2014 to 2024 were retrieved from the Web of Science Core Collection and PubMed databases. CiteSpace and VOSviewer were utilized to display the research patterns.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A bibliometric analysis was conducted on 4638 relevant publications, of which 3450 research articles and 1188 reviews were examined. China and the United States have contributed greatly to the research on the relationship between the oral microbiota and cancer, most of which were published in <i>Frontiers in Microbiology</i>, <i>Frontiers in Cellular and Infection Microbiology</i>, and <i>International Journal of Molecular Sciences</i>. The Chinese Academy of Sciences (107), Sichuan University (81), and Zhejiang University (79) are the most productive institutions. Zhou Xuedong, Hao Zhang, and Christian C. Abnet are the most recognized authors. Keyword co-occurrence revealed that the terms microbiome, oral microbiota, oral cancer, inflammatory diseases, probiotics, and dysbiosis are research hotspots in the past 10 years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The knowledge map provides a helpful visual representation of the main relevant topics in research conducted in the last 10 years on the relationship between the oral microbiome and cancer. The findings imply that oral squamous cell carcinoma, \u0000 <i>F. nucleatum</i>\u0000 , biomarkers, dysbiosis, and cancer treatment therapies have become popular topics in recent years.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"613-620"},"PeriodicalIF":2.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TROP2 Expression in Salivary Gland Adenoid Cystic Carcinoma (ACC) According to Histologic Subtype: Therapeutic Implications","authors":"Juliana Mota Siqueira,&nbsp;Yoshitsugu Mitani,&nbsp;Mario L. Marques-Piubelli,&nbsp;Camilla Oliveira Hoff,&nbsp;Flavia Bonini,&nbsp;Luana Guimaraes de Sousa,&nbsp;Mutsumi Mitani,&nbsp;Giovanna Lopes Carvalho,&nbsp;Fabio Daumas Nunes,&nbsp;Leandro Luongo Matos,&nbsp;Shiaw-Yih Lin,&nbsp;Michael T. Spiotto,&nbsp;Ehab Y. Hanna,&nbsp;Daniel J. McGrail,&nbsp;Adel K. El-Naggar,&nbsp;Renata Ferrarotto","doi":"10.1111/jop.70008","DOIUrl":"10.1111/jop.70008","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Adenoid cystic carcinoma (ACC) is a common salivary gland carcinoma with high recurrence and distant metastasis rates. Currently, there is no standard systemic treatment available. TROP2 is a transmembrane glycoprotein involved in the oncogenesis of several tumors that can be therapeutically targeted by a TROP2-antibody–drug conjugate (ADC). We aimed to characterize TROP2 expression in ACC and assess TROP2 as a potential therapeutic target.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>TROP2 immunohistochemistry was performed in a tissue microarray including 165 ACC of salivary gland. The tumors were grouped according to the histological pattern as non-solid, solid + non-solid, or solid. TROP2 protein expression in ACC cell lines was assessed and subjected to drug screening with TROP2-ADC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>TROP2 expression was high in 59%, moderate in 30%, weak in 8%, and negative in 3% of cases. TROP2 expression was significantly higher in non-solid compared with solid or solid + non-solid (<i>p</i> &lt; 0.001). Notably, TROP2 expression was heterogenous among the dual cellular component, with TROP2 expression identified predominantly in the ductal and not in the myoepithelial cells. In vitro drug screening demonstrated that TROP2-ADC had selective anti-tumor effect in TROP2 expressing ACC cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>TROP2 expression is prevalent in ACC, particularly in the ductal cell component of the non-solid tumors. The pre-clinical drug screening findings provide a biological rationale for exploring TROP2 as a therapeutic target in TROP2-expressing ACC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>\u0000 clinicaltrials.gov: NCT05884320; NCI-2023-04260</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"658-666"},"PeriodicalIF":2.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of Cytological Methods in Early Detection of Oral Squamous Cell Carcinoma and Potentially Malignant Disorders: A Systematic Review and Meta-Analysis","authors":"Hoda Tayebi-Hillali,&nbsp;Alejandro I. Lorenzo-Pouso,&nbsp;Xabier Marichalar-Mendía,&nbsp;Pilar Gándara-Vila,&nbsp;Dolores Reboiras-López,&nbsp;Andrés Blanco-Carrión,&nbsp;Martina Coppini,&nbsp;Vito Carlo Alberto Caponio,&nbsp;Mario Pérez-Sayáns","doi":"10.1111/jop.70010","DOIUrl":"10.1111/jop.70010","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) carries significant global mortality rates. Brush cytology presents a potential adjunctive tool for early detection and monitoring of OSCC and oral potentially malignant disorders (OPMDs). This study aims to systematically evaluate the diagnostic accuracy of cytology for detecting OSCC and OPMDs compared to histopathology as the reference standard. We conducted a systematic review and meta-analysis following PRISMA-DTA guidelines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>We searched PubMed, Embase, Scopus, Cochrane Library, and Web of Science from inception to January 2023 (updated in March 2025). Eligible studies included cohort studies evaluating cytology versus histopathological diagnosis. Two reviewers independently screened studies, extracted data, and assessed risk of bias using QUADAS-2. We used the Hierarchical Summary Receiver Operating Characteristic model for meta-analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 2603 identified studies, 53 met inclusion criteria, comprising 13,249 patients. Cytology demonstrated a pooled sensitivity of 0.914 (95% CI: 0.878–0.941) and specificity of 0.960 (95% CI: 0.937–0.975). The diagnostic odds ratio was 137.502 (95% CI: 79.733–237.127), with a positive likelihood ratio of 11.970 (95% CI: 9.005–15.912) and negative likelihood ratio of 0.096 (95% CI: 0.059–0.158). Subgroup analysis showed improved performance when exfoliative cytology was combined with DNA analysis or when using a metal spatula. Both conventional and liquid-based cytology were effective, with the latter showing modest advantages. Heterogeneity was substantial across studies (I² = 86.26%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Cytology demonstrates good diagnostic accuracy for detecting OSCC and OPMDs and may serve as a valuable adjunctive screening tool. However, it does not replace histopathological examination as the diagnostic gold standard. Further research should focus on standardizing collection techniques and interpretation criteria. Registration: PROSPERO CRD42023438610.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"507-527"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on Regulating OSCC Cell Function by Blocking Integrin αvβ3 With Cilengitide","authors":"Liujun Zeng,&nbsp;Huanquan Nie,&nbsp;Haofeng Xiong,&nbsp;Zhimin Yang,&nbsp;Xin Hu,&nbsp;Tong Su","doi":"10.1111/jop.70004","DOIUrl":"10.1111/jop.70004","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is the most important histologic type of oral tumor. Integrin- and integrin-dependent processes are involved in almost every step of cancer progression. Integrin αvβ3 was initially considered an integrin associated with tumor angiogenesis. With the gradual deepening of research, it was found that αvβ3 also plays a role in tumor cells. This study aimed to explore the function of tumor cell integrin αvβ3 in the progression of OSCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Immunohistochemical methods in OSCC tissues explored the expression of integrin αvβ3. CCK-8, EdU, Cell cycle assay, Scratch test, Transwell, Cell adhesion assay, Colony formation assay, Apoptosis assay, and in vivo experiment were performed to investigate the effects of integrin αvβ3 on the biological behaviors of OSCC cells such as proliferation, adhesion, migration, and invasion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In this study, we found that integrin αvβ3 expression was upregulated in OSCC. The inhibition of integrin αvβ3 expression by cilengitide resulted in the inhibition of proliferation, adhesion, migration, and invasion of OSCC cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Integrin αvβ3 regulates cell proliferation by affecting the cell cycle. Furthermore, inhibition of integrin αvβ3 expression significantly suppresses migration and invasion of OSCC cells in vitro and in vivo.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"597-606"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}