Journal of Oral Pathology & Medicine最新文献

{"title":"miR-134-3p Deficiency-Induced Methionine Metabolism Promotes Fibrosis via SLC25A33 in OSF","authors":"Mian-Feng Yao,&nbsp;Meng-Ying Shao,&nbsp;Qiu-Lan Li,&nbsp;Tian-Ru Yang,&nbsp;Zi-Ran Zheng,&nbsp;Xin-Le Zhang,&nbsp;Jia-Jun Huang,&nbsp;Yuan-Xin Sun,&nbsp;Jing He,&nbsp;Jiang Li,&nbsp;Chang-Yun Fang","doi":"10.1111/jop.70003","DOIUrl":"10.1111/jop.70003","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Fibrosis is a disease characterized by excessive collagen deposition by fibroblasts, leading to tissue and organ dysfunction. Fibroblasts are the primary effector cells, and their functional phenotype is regulated by various factors, with metabolic reprogramming being a crucial one. Previous studies have shown that microRNAs induce hypoxia, abnormal energy metabolism, and promote fibrosis. Our research aimed to identify the miRNA associated with oral submucous fibrosis (OSF) pathogenesis and to preliminarily explore its role in the phenotypic transformation of fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>RNA-seq was used to analyze the differential expression of miRNA in OSF and normal tissues, followed by validation in clinical samples (<i>n</i> = 14), saliva (<i>n</i> = 65), and animal models (<i>n</i> = 16). The effect of hsa-miR-134-3p on collagen synthesis was detected by qPCR. Bioinformatic, immunoblot, and Elisa methods were used to analyze the correlation between hsa-miR-134-3p and methionine metabolism, as well as the mTOR pathway. Finally, the luciferase reporter gene assay was used to identify the target gene of hsa-miR-134-3p.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Hsa-miR-134-3p is underexpressed in fibrotic tissues. Arecoline can promote collagen synthesis by reducing hsa-miR-134-3p. Bioinformatic analysis indicated that hsa-miR-134-3p is associated with methionine metabolism and mTOR signaling, and immunoblot and ELISA methods revealed that hsa-miR-134-3p regulates methionine metabolism via the mTOR pathway. Ultimately, hsa-miR-134-3p was identified to directly target SLC25A33 in fibroblasts using the luciferase reporter gene assay.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In summary, the findings suggested that miR-134-3p is underexpressed in fibroblasts, which directly binds to SLC25A33, leading to degradation of SLC25A33 mRNAs. SLC25A33 induces methionine metabolism reprogramming to promote collagen synthesis through the mTOR pathway in OSF.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"584-596"},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of Oral Spindle Cell and Pleomorphic Lipomas From Their Mimics via Retinoblastoma Status Through Immunohistochemistry and Fluorescence In Situ Hybridization","authors":"Hiromasa Hasegawa,&nbsp;Yoshihiko Sugita,&nbsp;Takanaga Ochiai,&nbsp;Rita Rani Roy,&nbsp;Katsumitsu Shimada,&nbsp;Motohiko Nagayama,&nbsp;Hatsuhiko Maeda","doi":"10.1111/jop.70007","DOIUrl":"10.1111/jop.70007","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The incidence of spindle cell/pleomorphic lipomas ranges from 2.2% to 47.2%. Its diagnosis is impeded by its diverse histological features, including adipocytes and myxo-collagenous stroma. Accordingly, we aimed to reexamine oral adipocytic tumors, focusing on <i>RB1</i>-deficient tumors, to reveal the diversity of oral spindle cell lipomas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We selected 30 cases of spindle cell/pleomorphic lipomas and their mimics from 111 oral adipocytic tumors. Subsequently, we performed analysis using immunohistochemistry for CD34 and RB protein (pRB) and <i>RB1</i> fluorescence in situ hybridization (FISH) in limited cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among the included cases, 16 were CD34-positive with pRB and/or the <i>RB1</i> loss. Among them, 15 were spindle cell lipomas (SCLs) with 27% of them being confirmed through FISH in cases where pRB status was mosaic. These included five low-fat SPLs (including one low-fat pleomorphic lipoma), four conventional SCLs, three fibrous SCLs, two myxoid, and one fat-rich SCL. The remaining case was an atypical spindle cell lipomatous tumor. Only prominent spindle cell and myxoid changes showed significant differences between SCL and fibrous lipoma. Two cases, initially identified as spindle cell lipomas, were reclassified as fibrolipoma and lipoma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Oral spindle cell/pleomorphic lipomas frequently exhibit prominent collagenous stroma, which results in low-fat and fibrous variants that require careful differentiation from fibrous lipomas or non-adipocytic tumors. Given the diverse histological findings, meticulous diagnosis of rare spindle cell lipoma variants and other lipomas in daily surgical pathology is essential. Specifically, it should apply CD34 and retinoblastoma protein immunohistochemistry, as well as <i>RB1</i> FISH when necessary.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"565-573"},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Transfection With Anti-Fibrotic microRNAs for Treating Oral Submucous Fibrosis","authors":"Chih-Yuan Fang,&nbsp;Yin-Ju Chen,&nbsp;Kuan-Chou Lin,&nbsp;Yueh-Hsin Chen,&nbsp;Kai-Chiang Yang","doi":"10.1111/jop.70009","DOIUrl":"10.1111/jop.70009","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral submucous fibrosis is characterized by excessive collagen deposition and is highly associated with a patient's betel nut chewing habit. Arecoline initiates the transforming growth factor-beta (TGF-β)/Smads signaling pathway and activates downstream fibrotic genes. Dysregulation of microRNA (miR) expression is involved in the OSF progression, and miR modulation is a promising treatment. As one miR can target multiple mRNAs, and one mRNA has multiple binding sites to different miRs, we thus propose that simultaneous co-transfection of anti-fibrotic miRs may have a better therapeutic effect than single miR transfection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Human oral fibroblasts were first subjected to arecoline stimulation and then transfected with 16 miRs individually. Based on the ability to downregulate <i>TGFB1</i> and actin alpha 2, smooth muscle (<i>ACTA2</i>) mRNA levels, the miR-29a-3p mimic, miR-196a-3p mimic, and miR-509-5p mimic were selected for co-transfection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In addition to downregulation of collagen type I alpha 1 chain (<i>COL1A1</i>), <i>COL3A1</i>, <i>COL5A1</i>, matrix metalloproteinase-1 (<i>MMP1</i>), <i>MMP7</i>, tissue inhibitor of metalloproteinase-1 (<i>TIMP1</i>), and <i>TIMP2</i> mRNA expressions, co-transfection with the three miRs led to a more significant downregulation of <i>COL1A1</i> and <i>MMP1</i> expressions. A Western blot analysis revealed that co-transfection of the miRs efficiently suppressed the TGF-β/Smads pathway and extracellular matrix component productions. Furthermore, co-transfection with miRs more effectively inhibited wound closure and collagen gel contraction compared to single miR transfection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Co-transfection of anti-fibrotic miRs can be a promising treatment for oral submucous fibrosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"574-583"},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoral Basal Cell Carcinoma: Controversies and Insights From a Systematic Review","authors":"Antonia Taiane Lopes de Moraes,&nbsp;Lauren Frenzel Schuch,&nbsp;Pablo Agustin Vargas,&nbsp;Vivian Petersen Wagner","doi":"10.1111/jop.70005","DOIUrl":"10.1111/jop.70005","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The existence of intraoral basal cell carcinoma (IOBCC) remains controversial, despite reported cases in the literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A systematic search of four electronic databases and grey literature was conducted. Strict inclusion criteria were established to define a true case of IOBCC, including Ber-EP4 positivity to distinguish it from peripheral ameloblastoma and the absence of a history of skin lesions—either nearby, which could indicate direct infiltration, or at distant sites, which could suggest metastasis rather than a true primary IOBCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 9 cases met the inclusion criteria. IOBCC lesions were located on the palate, buccal mucosa and gingiva, with no cases involving the tongue or floor of the mouth—common sites for squamous cell carcinoma. Histologically, islands of basaloid cells with peripheral palisading and stromal retraction were the predominant findings. All cases were Ber-EP4 positive. Six patients remained disease-free during follow-up, one experienced multiple recurrences, and two had no follow-up data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>IOBCC is extremely rare, with few documented cases. While previous studies suggested the gingiva as a common site, this review found no clear predilection, and some previously reported cases may have been misdiagnosed as peripheral ameloblastoma. Ber-EP4 positivity in IHC is valuable for distinguishing between these entities. Additionally, by excluding cases with potential infiltration from skin lesions, this review underscores the importance of a thorough dermatological evaluation to rule out cutaneous extension or metastasis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"498-506"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Described Radiographic Characteristics of Bone Lesions Should Reflect Recent Technological Advances in Oral Radiology","authors":"Eduardo Murad Villoria,&nbsp;Thaiza Gonçalves Rocha,&nbsp;Felipe Rodrigues Tosto,&nbsp;Lara Piê Ventura Mendonça,&nbsp;Maria Augusta Visconti","doi":"10.1111/jop.70006","DOIUrl":"10.1111/jop.70006","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To alert the scientific community about the emergence of new characteristics of bone lesions of the maxillomandibular complex visualized in newer cone-beam computed tomography (CBCT) technologies, as well as in current quantitative methods of image analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This Letter to the Editor was based on the authors' professional experience, supported by scientific articles that described the characteristics of bone lesions of the maxillomandibular complex visualized in two-dimensional (2D) and three-dimensional (3D) imaging methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Bone lesion patterns present in the maxillomandibular complex can vary between 2D and 3D exams, as well as between different protocols and generations of CBCT devices. Additionally, the radiomic analysis in CBCT allows a quantitative characterization of bone lesions, presenting potential to be a non-invasive method for differential diagnosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Oral radiology undergoes constant technological modernization, resulting in improvements in image quality and revealing new behavior patterns of the bone lesions visualized in 3D images, especially in CBCT. Authors responsible for upcoming publications in oral pathology and radiology should be willing to research and describe possible new qualitative and quantitative features of bone lesions affecting the maxillomandibular complex.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"485-486"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Information Provided in Remote Case Discussions on Oral Medicine Teleconsultants' Diagnostic Impression","authors":"Liliana Wolf Braun,&nbsp;Eduarda Soares,&nbsp;Alan Roger Santos-Silva,&nbsp;Liliane Janete Grando,&nbsp;Luiz Alcino Monteiro Gueiros,&nbsp;Maria Inês Meurer,&nbsp;Márcio Ajudarte Lopes,&nbsp;Cassius Carvalho Torres-Pereira,&nbsp;Janete Dias Almeida,&nbsp;Ricardo Alves Mesquita,&nbsp;Vinicius Coelho Carrard","doi":"10.1111/jop.70001","DOIUrl":"10.1111/jop.70001","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>To evaluate the impact of case descriptions, alongside clinical photographs, on telediagnosis accuracy in Oral Medicine.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>An analytical cross-sectional observational study used 100 cases from Oral Medicine services at three universities. Three specialists in Oral Diagnosis remotely analyzed these cases. Simulated teleconsultations were presented via Google Forms, requesting: (a) diagnostic hypotheses based solely on clinical photographs; (b) identification of essential information for diagnosis; and (c) revised hypotheses after reviewing case descriptions. The specialists' opinions, with and without case descriptions, were compared to in-person diagnoses and categorized as: negative impact, maintenance of incorrect opinion, maintenance of correct opinion, and refinement of the response.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Diagnostic accuracy based on images alone were 84%, 83%, and 75%. There was no statistically significant difference in accuracy before and after accessing case descriptions. Although these descriptions were not essential for a correct diagnosis, they contributed to refining diagnostic hypotheses in some cases. The most frequently requested information was the “duration of the condition” (84% of cases).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Experienced Oral Diagnosis specialists demonstrate satisfactory diagnostic accuracy when analyzing cases of oral lesions from a distance using photos, even when no clinical information is provided about the cases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"556-564"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Role of Artificial Intelligence in Oral Pathology: Diagnostic and Prognostic Implications","authors":"Ibrahim S. Alsanie,&nbsp;Ahmed Qannam,&nbsp;Ibrahim O. Bello,&nbsp;Syed Ali Khurram","doi":"10.1111/jop.70002","DOIUrl":"10.1111/jop.70002","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This narrative review aims to briefly discuss the concepts of artificial intelligence (AI), explore its role in oral pathology and oncology, and shed light on AI's potential in addressing diagnostic and prognostic challenges in the field. Additionally, future perspectives of AI are postulated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A literature search using PubMed, EMBASE, Web of Science, and Scopus for studies published before February 2025 related to AI usage in the assessment of oral diseases such as oral potentially malignant disorders and transformation to oral cancer, oral squamous cell carcinoma, salivary gland tumors, and odontogenic tumors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Numerous investigative efforts have been directed at AI applications for diagnostic, risk assessment, and prognostication of oral diseases, although these are still relatively in their infancy, and more still needs to be done to refine these tools to make them overcome all the challenges that are being encountered.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>While AI does not seek to replace oral pathologists but supplement their efforts, it has an immense potential to revolutionize oral pathology practice in the years to come. AI applications would greatly improve diagnostic accuracy, enhance epidemiological surveillance of diseases, drive the profession toward more personalized patient care and ultimately improve patient outcomes beyond the current level.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"487-497"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herpes Simplex Treated With Antimicrobial Photodynamic Therapy: Randomized Controlled Trial","authors":"Andreia La Selva,&nbsp;Daniella Teixeira Bezerra,&nbsp;Rodrigo Melim Zerbinati,&nbsp;Marcela Leticia Leal Gonçalves,&nbsp;Ricardo Scarparo Navarro,&nbsp;Marina Stella Bello-Silva,&nbsp;Ellen Sayuri Ando-Suguimoto,&nbsp;Ana Cecília Corrêa Aranha,&nbsp;Paulo Henrique Braz-Silva,&nbsp;Karen Muller Ramalho,&nbsp;Lara Jansiski Motta,&nbsp;Kristianne Porta Santos Fernandes,&nbsp;Sandra Kalil Bussadori,&nbsp;Raquel Agnelli Mesquita-Ferrari,&nbsp;Céline Frochot,&nbsp;Anna Carolina Ratto Tempestini Horliana","doi":"10.1111/jop.13647","DOIUrl":"10.1111/jop.13647","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To compare the efficacy of antimicrobial photodynamic therapy (aPDT) with topical acyclovir in terms of healing time for herpes simplex lesions, recurrence rates, HSV-1 quantification in secretion and saliva, pain levels, temperature changes, and outcomes measured by the Oral Health Impact Profile Questionnaire (OHIP-14).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Subjects and Methods</h3>\u0000 \u0000 <p>Patients with vesicle or ulcer phase lesions were randomly divided into two groups: the control group (topical acyclovir four times daily for 7 days + aPDT simulation, <i>n</i> = 12) and the experimental group (placebo acyclovir ointment + aPDT applied once, <i>n</i> = 12). The primary outcome was lesion resolution time, with secondary assessments for recurrence, pain, local temperature, virus quantification via quantitative polymerase chain reaction (qPCR), and impact on quality of life.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After 1 year, the remission and recurrence rates were similar between groups (<i>p</i> = 0.718 and <i>p</i> = 0.317, respectively), as were pain levels and temperature (<i>p</i> = 0.039 and 0.217). There was an increase in HSV-1 viral load in saliva, measured by qPCR, on Day 3 in the ACV group (<i>p</i> = 0.043). The OHIP-14 scores were similar between groups after 1 year.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study found no differences between aPDT and topical acyclovir regarding lesion resolution time, recurrence rates, pain levels, local temperature changes, and OHIP-14 scores. Both treatments showed similar efficacy in managing herpes simplex lesions over 1 year. While there was an increase in HSV-1 viral load in saliva in the acyclovir group on Day 3, this did not translate into differences in clinical outcomes between the groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>NCT 04037475</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"547-555"},"PeriodicalIF":2.3,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Assessment of Oral Leukoplakia by Individual Dysplasia Features","authors":"Andresa Borges Soares,&nbsp;Saman Warnakulasuriya,&nbsp;Letícia da Silveira Terra Junqueira,&nbsp;André Luis Santana de Freitas,&nbsp;Amanda Schneider,&nbsp;Alan Roger Santos Silva,&nbsp;Marcelo Sperandio","doi":"10.1111/jop.13633","DOIUrl":"10.1111/jop.13633","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Malignant transformation (MT) risk assessment in oral leukoplakia (OL) relies on tissue sample and oral epithelial dysplasia (OED) grading. The aim of this study was to evaluate the role of each OED feature in predicting MT in OL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Subjects and Methods</h3>\u0000 \u0000 <p>Ninety-nine OL patients were selected (81 non-transforming and 18 with MT). All OED features were individually scored for each case. Follow-up data were obtained from both local and regional cancer registries. Spearman correlation, logistic regression, and Kaplan–Meier were tested with MT as outcome. Thresholds for number of features indicating risk and predictive values (PV) were calculated. A random forest (RF) model was built to assess the relevance of each feature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Loss of epithelial cohesion, increased nucleoli, and inflammation were the associated with MT (<i>p</i> ≤ 0.016). Combining these three features yielded high specificity (95%) and PV (50% positive and 85% negative). Using a six-feature threshold to establish risk reached 54%, 26% and 90%, and 72%, respectively. Specifying and counting OED features proved crucial to establishing risk.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Loss of epithelial cohesion, increased size and number of nucleoli, and inflammation are key risk features and sum of OED features is the most useful predictor of MT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"537-546"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological Significance and Prognostic Value of the Caveolae Constitutive Proteins Dynamin-2 and Caveolin-1 in Oral Squamous Cell Carcinoma","authors":"Yoshiaki Kishikawa,&nbsp;Koroku Kato,&nbsp;Kyosuke Hakoda,&nbsp;Hayato Funaki,&nbsp;Hisano Kobayashi,&nbsp;Yutaka Kobayashi,&nbsp;Hiroki Miyazawa,&nbsp;Natsuyo Noguchi,&nbsp;Shuichi Kawashiri","doi":"10.1111/jop.13646","DOIUrl":"10.1111/jop.13646","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>We investigated the clinicopathological significance of the expression of two caveolae component proteins, dynamin-2 (DNM2) and caveolin-1 (CAV1), in primary tumors of patients with oral squamous cell carcinoma (OSCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Immunohistochemical staining for DNM2 and CAV1 was performed on resected primary tumor specimens from 80 OSCC patients, and the individual expressions and combined expression status of these proteins were analyzed in relation to clinicopathological factors and prognosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We observed that the DNM2 expression was significantly correlated with the OSCC T-classification and the stage, while CAV1 expression was significantly correlated with the mode of invasion and recurrence. Moreover, the combined DNM2/CAV1 expression status was significantly correlated with the T-classification, stage, cell differentiation, and recurrence. In terms of overall survival, the CAV1-positive patients had a significantly poorer prognosis compared to the CAV1-negative patients, and the patients who expressed neither DNM2 nor CAV1 had a significantly better prognosis than those expressing either or both proteins.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These results suggest that in OSCC, the expression of DNM2 is involved in tumor growth and the expression of CAV1 is involved in tumor invasion, and DNM2 and/or CAV1 expression affects the progression and prognosis of OSCC. The expressions of DNM2 and CAV1 may therefore be useful markers for OSCC progression and prognosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 7","pages":"528-536"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.13646","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}