Journal of Oral Pathology & Medicine最新文献

{"title":"Effects of Clarified Açaí Supplementation and Photobiomodulation on the Parotid Glands of Rats Submitted to Chemotherapy.","authors":"Wallacy Watson Pereira Melo, Walessa Alana Bragança Aragão, Maria Karolina Martins Ferreira, Vinicius Ruan Neves Dos Santos, Leonardo Oliveira Bittencourt, Paulo Fernando Santos Mendes, Deborah Ribeiro Frazão, José Mário Matos-Sousa, Hadassa Helez Neves Ferreira, José Messias Perdigão, Hannah Gil de Farias Morais, Herve Rogez, Roseana de Almeida Freitas, Manoela Domingues Martins, Rafael Rodrigues Lima, Renata Duarte de Souza-Rodrigues","doi":"10.1111/jop.70072","DOIUrl":"https://doi.org/10.1111/jop.70072","url":null,"abstract":"<p><strong>Background: </strong>The antineoplastic drugs used in anticancer treatment regimens can induce changes in normal tissues, including salivary glands. This study evaluated the effects of clarified açaí and photobiomodulation (PBM) on the oxidative biochemistry and microstructure of the parotid glands of rats with oral mucositis.</p><p><strong>Methods: </strong>Male rats (n = 54) were divided into five groups: Negative control (no mucositis); Positive control (mucositis without treatment); PBM; Clarified açaí; and PBM + clarified açaí. Oral mucositis was induced by intraperitoneal injection of 5-fluorouracil on days 0 (60 mg/kg) and 2 (40 mg/kg). On days 3 and 4, bilateral scarification of the buccal mucosa was performed. On days 0 (negative controls), 8 and 10 (other groups), parotid glands were collected. To evaluate oxidative biochemistry, the following analyses were performed: Antioxidant capacity test against peroxyl radicals (ACAP), Lipid Peroxidation Assay (LPO), and Nitric oxide metabolite (NOx). Additionally, histopathological, histomorphometric, and histochemical analyses were performed. Statistical analysis was performed using two-way ANOVA and Tukey's post-test (p < 0.05).</p><p><strong>Results: </strong>Clarified açaí, alone or associated with photobiomodulation, increased antioxidant levels compared to the positive control on days 8 and 10. Regarding lipid peroxidation and nitric oxide metabolite, the positive control showed higher levels than the other groups. The morphological assessment showed that the clarified açaí and photobiomodulation groups maintained similar structures of the parenchyma, stroma, and acini to the negative control.</p><p><strong>Conclusions: </strong>The study results demonstrated that clarified açaí and photobiomodulation conferred biochemical and structural protection to the parotid glands against chemotherapy-induced damage.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145355065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Intervention of Subchondral Bone Resorption Mitigates Cartilage Degeneration in TMJOA.","authors":"Meng-Nan Cao, Shi-Yang Feng, Chen-Chen Gao, Yang Xiao, Yi-Xin Li, Jie Lei, Kai-Yuan Fu","doi":"10.1111/jop.70073","DOIUrl":"https://doi.org/10.1111/jop.70073","url":null,"abstract":"<p><strong>Background: </strong>Excessive subchondral bone resorption is a typical manifestation in the early stage of osteoarthritis. This study is to verify whether and when intervening in subchondral bone could alleviate cartilage degeneration of temporomandibular joint osteoarthritis.</p><p><strong>Methods: </strong>Disc displacement without reduction was used to induce temporomandibular joint osteoarthritis. Alendronate was administered subcutaneously twice a week at a dosage of 60 μg/kg body weight for 1 week by two intervention methods: early administration (1 day after disc displacement without reduction surgery) and late administration (2 weeks after disc displacement without reduction surgery). Micro-CT was used to assess subchondral bone mass and microstructure. Hematoxylin-eosin staining, toluidine blue staining, tartrate-resistant acid phosphatase staining, immunofluorescence staining, and Western blot were applied to evaluate histopathological changes of the condylar cartilage and subchondral bone.</p><p><strong>Results: </strong>Early alendronate administration not only prevented subchondral bone resorption in early-stage temporomandibular joint osteoarthritis, but also suppressed chondrocyte apoptosis, cartilage extracellular matrix degeneration, as well as excessive subchondral bone formation of condyle in late-stage temporomandibular joint osteoarthritis. However, late alendronate administration had little effect on either subchondral bone or cartilage degenerative changes.</p><p><strong>Conclusions: </strong>Early inhibition of subchondral bone resorption could mitigate abnormal subchondral bone formation and condylar cartilage degeneration in late-stage temporomandibular joint osteoarthritis, which might be a promising strategy for temporomandibular joint osteoarthritis treatment.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145345679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Panax notoginseng Saponins Alleviate OSF by Inhibiting Ferroptosis Through GPX4 Activation.","authors":"Xinyue Zhang, Yujie Sun, Chenxi Zhao, Hong Zou, Liang Hu, Lixiang Wen, Qun Tang","doi":"10.1111/jop.70069","DOIUrl":"https://doi.org/10.1111/jop.70069","url":null,"abstract":"<p><strong>Objectives: </strong>Oral submucous fibrosis (OSF) is a chronic progressive disease characterized by fibrosis. Panax notoginseng saponins (PNS) are effective components of the traditional Chinese medicine Panax notoginseng and play an important role in the treatment of OSF. However, the underlying mechanism of action for this medicine remains unclear. The aim of this study was to explore the effects of ferroptosis in OSF.</p><p><strong>Subjects and methods: </strong>The mice were divided into six groups. Mouth openings were detected. The buccal tissues were harvested for histomorphological analysis, western blot, and RT-qPCR. The cells were harvested for CCK8 detection, western blot, RT-qPCR, TEM, immunofluorescence, fluorescent probe, and colorimetry.</p><p><strong>Results: </strong>PNS improved mouth opening, alleviated tissue cell damage, and inhibited ferroptosis and fibrosis in OSF rat models. Our study found that ferroptosis is closely related to arecoline-induced OSF and that PNS inhibits ferroptosis by upregulating glutathione peroxidase 4 (GPX4) to alleviate OSF.</p><p><strong>Conclusions: </strong>The research established OSF in vivo and in vitro, respectively, and the changes in the expression of fibrosis and ferroptosis-related markers in these models were studied to provide a theoretical and experimental basis for the clinical prevention and treatment of OSF.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemerin, OPG, and WPOI as Markers of Bone Invasion and Prognosis in Oral Squamous Cell Carcinoma.","authors":"Jonas Eichberger, Juliane Schmelzer, Michael Gerken, Christa Buechler, Daniela Schulz, Mathias Fiedler, Stephanie Eckmueller, Josef Maximilian Gottsauner, Richard Bauer, Torsten Eugen Reichert, Florian Weber, Tobias Ettl","doi":"10.1111/jop.70068","DOIUrl":"https://doi.org/10.1111/jop.70068","url":null,"abstract":"<p><strong>Background: </strong>The objective of our study was to examine the role of Chemerin, Chemokine like receptor 1 (CMKLR1), receptor activator of nuclear factor kappa-Β ligand (RANKL), and Osteoprotegerin (OPG) in the development of bone-invasive oral squamous cell carcinoma.</p><p><strong>Methods: </strong>In order to evaluate the presence of these markers at the interface between bone and tumor, immunohistochemical analyses were conducted using tissue microarrays obtained from 164 patients with oral squamous cell carcinoma growing in close contact with jaw bone.</p><p><strong>Results: </strong>The findings indicate that Chemerin and Osteoprotegerin are notably reduced in tumors that have invaded the bone. Only 21 (32.8%) of pT4a tumors (defined as bone invasive) had a high Osteoprotegerin expression, whereas 36 (66.7%) of pT2 and pT3 tumors demonstrated high expression of Osteoprotegerin (p < 0.001). Similarly, we saw a downregulation of Chemerin in 50 (60.2%) bone invasive oral squamous cell carcinoma samples compared to 28 (35.0%) in non-bone invasive tumors (p = 0.002). In addition, our data indicated a connection between worst pattern of invasion score and less favorable overall and disease-specific survival (p = 0.007 and p = 0.024, respectively).</p><p><strong>Conclusions: </strong>The findings suggest that Chemerin and Osteoprotegerin have the potential to serve as indicators for bone invasion in oral squamous cell carcinoma, which could have significant implications for diagnosis and treatment approaches.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Features Associated With Malignant Transformation of Low-Grade Dysplasia.","authors":"Denise M Laronde, Matt Berkowitz, A Ross Kerr, Erinn M Hade, Mutita Siriruchatanon, Miriam P Rosin, Stella K Kang","doi":"10.1111/jop.70070","DOIUrl":"https://doi.org/10.1111/jop.70070","url":null,"abstract":"<p><strong>Background: </strong>Inferring risk for malignant transformation (MT) in patients with lesions diagnosed as mild or moderate oral epithelial dysplasia (low-grade OED) remains challenging. We developed two models assessing the risk of progression to high-grade OED (severe dysplasia or carcinoma in situ) or OSCC in patients with low-grade OED lesions.</p><p><strong>Methods: </strong>We included demographic, risk habit and clinical data from participants with low-grade OED lesions enrolled in the BC Oral Cancer Prevention Program's Oral Cancer Prediction Longitudinal study. Cox proportional hazard models were fit to estimate the effects of anatomic site and toluidine blue findings and adjusted for confounders, as both are associated with MT in the literature but without a North American-specific cohort analysis. Our primary model included both variables of interest. A secondary model included only anatomic site since toluidine blue is not in widespread use.</p><p><strong>Results: </strong>Five hundred and thirty-four participants with 605 lesions met final inclusion criteria, with 339 mild and 266 moderate OED at baseline. In the primary model, lesions at a high-risk anatomic site or with positive toluidine blue staining were associated with a 2.6 and 2.4-fold increased risk of progression, respectively. In the second model that did not incorporate toluidine blue, high-risk anatomic site remained a highly associated risk factor (2.7-fold increased risk of progression).</p><p><strong>Conclusion: </strong>Lesion anatomic site is associated with higher risk of MT for the general practitioner, while a specialist with access to toluidine blue results can assume additional risk associated with positive staining. These models may inform decisions for surveillance and intervention for OED.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pemphigus Vulgaris Scoring Systems: A Scoping Review.","authors":"Sue-Ching Yeoh, Stephen Adelstein, Omar Kujan","doi":"10.1111/jop.70071","DOIUrl":"https://doi.org/10.1111/jop.70071","url":null,"abstract":"<p><strong>Background: </strong>Pemphigus vulgaris is a rare autoimmune blistering condition characterised by mucocutaneous lesions secondary to acantholysis. Assessment of disease activity, severity, and treatment response is crucial for guiding management and research. Multiple clinical scoring systems have been developed for pemphigus vulgaris; however, consensus on their optimal use is lacking. This scoping review aims to identify, evaluate, and summarise the clinical scoring systems used in pemphigus vulgaris, focusing on validity, reliability, and application in clinical practice and research.</p><p><strong>Methods: </strong>A comprehensive literature search was conducted using electronic databases (PubMed (Medline), Embase, Web of Science, Scopus, and Cochrane Library) as well as grey literature to identify studies describing clinical scoring systems for pemphigus vulgaris. Data on scoring system components, validity, clinical applicability, and limitations were extracted and synthesised.</p><p><strong>Results: </strong>The review identified several scoring systems, including the Pemphigus Disease Area Index, Autoimmune Bullous Skin Disorder Intensity Score, and other less commonly used tools. Scoring systems varied in design, with key differences noted in assessment domains, including mucosal versus cutaneous involvement, patient-reported outcomes, and usability.</p><p><strong>Conclusion: </strong>Current clinical scoring systems for pemphigus vulgaris provide frameworks for disease assessment but exhibit variability in scope, validation, and practical implementation. Further development to incorporate emerging biomarkers and quality of life, as well as encompass all clinical subsites, will enhance their utility in guiding patient care and advancing research. This review highlights the need for consensus on a universal scoring system tailored to the multifaceted nature of pemphigus vulgaris.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological Profile of Intraosseous Adenoid Cystic Carcinoma of the Jaws: A Systematic Review.","authors":"Luccas Lavareze, João Figueira Scarini, Reydson Alcides de Lima-Souza, Talita de Carvalho Kimura, Rogério de Oliveira Gondak, Erika Said Abu Egal, Albina Altemani, Fernanda Viviane Mariano","doi":"10.1111/jop.70063","DOIUrl":"https://doi.org/10.1111/jop.70063","url":null,"abstract":"<p><strong>Objectives: </strong>We aimed to evaluate the clinicopathological features, survival rate, and potential prognostic markers of the jaws' primary intraosseous adenoid cystic carcinoma (PIACC).</p><p><strong>Materials and methods: </strong>MEDLINE/PubMed, Scopus, and Embase searches were performed with the keywords \"adenoid cystic carcinoma,\" and \"jaw,\" or \"maxilla,\" or \"mandible.\" We included articles that evaluated cases diagnosed as PIACC in jaws. Studies with insufficient demographic data, inconclusive histopathologic diagnosis, and appropriate follow-up were excluded. The Joanna Briggs Institute tool was used to assess the risk of bias.</p><p><strong>Results: </strong>Fifty-five PIACC comprising 27 studies met the inclusion criteria. The mean age of the patients was 56.4 ± 19.6 years with no sex predilection. PIACC showed a strong predilection for the mandible (69.1%), mainly in the posterior segment (40%). The patients presented symptoms in 87.3% of cases. Radiographically, PIACC presented as an ill-defined radiolucent lesion (40%). Most cases showed a cribriform pattern (32.7%). PIACC with a solid growth pattern presented a lower disease-free survival (DFS) (p = 0.040). The 2- and 5-year overall survival rates were 57.9% and 53.8%, respectively. Distant metastases were seen in 3.6% of the patients and were related to a lower DFS (p = 0.043).</p><p><strong>Conclusion: </strong>PIACC is a rare neoplasm of the jaws with an incidence in the fifth and sixth decades of life and no sex predilection. The posterior mandible was affected in most cases. Solid growth patterns and distant metastases are prognostic factors for a lower DFS.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated N6-Methyladenosine (m6A)-RNA-Methylation During Oral Carcinogenesis.","authors":"Zhiming Qin, Yanting Chi, Xinpei Wang, Jing Yan, Xinning Zhang, Binbin Li","doi":"10.1111/jop.70066","DOIUrl":"https://doi.org/10.1111/jop.70066","url":null,"abstract":"<p><strong>Background/purpose: </strong>Oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) encompass a series of molecular events in the malignant transformation process, ranging from simple epithelial hyperplasia to mild, moderate, and severe dysplasia. N6-methyladenosine (m6A)-RNA methylation participates in the regulation of the tumorigenesis of various malignant tumors, yet the roles played by m6A-RNA methylation in OED and OSCC remain unclear. Therefore, this study focused on investigating OED and OSCC from an epigenetic perspective, aiming to elucidate the underlying molecular mechanisms of malignant transformation.</p><p><strong>Materials and methods: </strong>Laser microdissection was performed on OED and OSCC samples. Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) and RNA sequencing (RNA-seq) were applied to establish comprehensive profiles of m6A methylation modifications and gene expression patterns and to identify differentially modified/expressed genes in OED and OSCC.</p><p><strong>Results: </strong>We presented the overall modification/expression profiles of m6A-RNA-methylation in OED and OSCC. Four hypermethylated genes and 11 hypomethylated genes were found in both OED and OSCC, together with the expression of 107 upregulated and 37 downregulated genes. The most common motifs GRAGRA (R = A/G) of the OED and OSCC methylation sites were mainly located in coding and stop codon regions. In the stable group, C4B, DNAH9, and NCALD all exhibited hypermethylated and upregulated, and the overall survival rate of patients with high expression of these genes was higher than that of patients with low-level expression.</p><p><strong>Conclusion: </strong>Our study revealed that the level of m6A-RNA methylation in the epithelial tissues of OED and OSCC was higher than that in oral normal epithelium, suggesting that the methylation modification might be involved in the occurrence of OED and its progression to OSCC. Furthermore, hypermethylation and upregulated expression of C4B, DNAH9, and NCALD were associated with a favorable prognosis in these diseases.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why Oral Health Deserves a Seat at the Global Health Table.","authors":"Chukwuemeka L Anyikwa, Peter A Brennan, Chukwuebuka E Ogwo","doi":"10.1111/jop.70065","DOIUrl":"https://doi.org/10.1111/jop.70065","url":null,"abstract":"<p><p>Oral diseases affect billions worldwide yet remain sidelined in both national and global health policies. This oversight has perpetuated disparities in care access, particularly in low- and middle-income countries. The separation of oral health from broader healthcare systems is illogical and dangerous, given oral health's deep connections to systemic health. This commentary calls for the integration of oral health into primary care and its recognition as a fundamental health right. Essential dental services must be incorporated into universal health coverage (UHC), financial barriers dismantled, and policy frameworks updated. A paradigm shift is essential to position oral health at the heart of global health policy and noncommunicable disease (NCD) prevention strategies.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Pulp Mesenchymal Stem Cell-Secretome Gel Reverse Areca Nut Induced Oral Submucous Fibrosis in Mice: A Pilot Study.","authors":"Nishant Mante, Supriya Kheur, Avinash Sanap, Avinash Kharat, Pranjali Potdar, Poonam Suryawanshi, Ravindra Badhe, Vaishali Undale, Nitika Monga, Ramesh Bhonde","doi":"10.1111/jop.70064","DOIUrl":"https://doi.org/10.1111/jop.70064","url":null,"abstract":"<p><strong>Objectives: </strong>Oral submucous fibrosis is a pre-malignant disorder caused by habitual areca nut consumption. This pilot study investigated the therapeutic potential of dental pulp mesenchymal stem cell-secretome chitosan gel in a mice model of areca nut extract-induced oral submucous fibrosis.</p><p><strong>Materials and methods: </strong>Angiogenic potential of dental pulp mesenchymal stem cell-secretome was validated using a chick yolk sac membrane assay. Oral submucous fibrosis was induced in male Swiss albino mice (n = 40) via intraoral areca nut extract administration for 3 months. Post-induction, animals were divided into disease control, DPMSCs-S, and DPMSCs-S gel groups. Treatments were administered intraorally twice weekly for 1 month. Therapeutic efficacy was assessed through measurements of mouth opening, histopathology, oxidative stress markers (LDH, MDA, SOD), and fibrotic gene expression (COL1, COL3, α-SMA).</p><p><strong>Results: </strong>Dental pulp mesenchymal stem cells' secretome contains pro-angiogenic growth factors. Dental pulp mesenchymal stem cells' secretome gel significantly improved mouth opening, restored epithelial architecture, and reduced collagen deposition. Histological staining and gene expression analyses confirmed the reversal of fibrosis and downregulation of COL1, COL3, and α-SMA. Additionally, the gel reduced LDH and MDA levels and enhanced SOD activity, indicating antioxidant effects. The gel showed superior efficacy over the secretome alone.</p><p><strong>Conclusion: </strong>DPMSCs-S gel demonstrates significant anti-fibrotic, antioxidant, and regenerative potential in reversing ANE-induced OSMF in mice. These findings warrant further investigation into larger, long-term preclinical studies.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}