Journal of Oral Pathology & Medicine最新文献

{"title":"The FUS/COL11A1/ZEB1 Axis Contributes to the Development of Oral Squamous Cell Carcinoma.","authors":"Tianyuan Zhou, Shuang Liu, Lixin Shi, Lei Zhang","doi":"10.1111/jop.13610","DOIUrl":"https://doi.org/10.1111/jop.13610","url":null,"abstract":"<p><strong>Objective: </strong>Oral squamous cell carcinoma (OSCC) is a common type of head and neck cancer with high metastasis rate and poor prognosis. This study aimed to explore the role of collagen type XI alpha 1 (COL11A1) and its detailed mechanisms in OSCC progression.</p><p><strong>Methods: </strong>Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis was used to measure the level of COL11A1, fused in sarcoma/translocated in liposarcoma (FUS) and zinc finger E-box binding homeobox 1 (ZEB1). The protein expression of COL11A1, E-cadherin, N-cadherin, FUS, and ZEB1 were detected using Western blot. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and 5-Ethynyl-2'-deoxyuridine (EdU) staining assays were conducted to measure cell proliferation. Cell migration and invasion were evaluated via wound-healing assay and transwell migration and invasion assays, respectively. Moreover, epithelial mesenchymal transition (EMT) was determined via detecting the expression of EMT-related proteins, including E-cadherin and N-cadherin. RNA immunoprecipitation assay was conducted to evaluate the relationship between ZEB1 and COL11A1. In vivo animal experiments were carried out to explore the role of COL11A1 in OSCC mice, and immunohistochemistry assay was performed to detect the level of ZEB1 and EMT-related proteins in tumor tissues from mice.</p><p><strong>Results: </strong>COL11A1 was augmented in OSCC tissues and cells. COL11A1 knockdown significantly inhibited cell proliferation, migration, invasion, and EMT in OSCC cells. FUS was increased in OSCC tissues. FUS stabilized the mRNA level of COL11A1 and positively regulated the protein expression of COL11A1. ZEB1 was highly expressed in OSCC tissues, and COL11A1directly targeted ZEB1. The inhibition effects of COL11A1 knockdown on cell proliferation, invasion, migration, and EMT were reversed by ZEB1 overexpression in OSCC cells. Additionally, COL11A1 depletion markedly repressed tumor growth through decreasing ZEB1 expression in vivo.</p><p><strong>Conclusion: </strong>FUS stabilized COL11A1 to promote the malignant progression of OSCC via regulating ZEB1 expression.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Ki-67 in the Invasive Zone of Oral Squamous Cell Carcinoma.","authors":"Ke Li, Shuai Wang, Zihui Li, Qiuya Yu, Lingyun Liu, Fuyan Li, Lei Zhang, Guowen Sun, Yanhong Ni","doi":"10.1111/jop.13608","DOIUrl":"https://doi.org/10.1111/jop.13608","url":null,"abstract":"<p><strong>Background: </strong>The molecular profile of cells within the tumor invasive zone, where tumor cells interact with surrounding non-tumor cells, plays a crucial role in defining tumor malignant characteristics, such as the pattern of invasion (POI). Therefore, evaluating the diagnostic value of the proliferation index molecule, Ki-67, in both tumor cells and adjacent non-tumor cells at the invasive zone with different POIs, holds significant clinical importance for oral squamous cell carcinoma (OSCC).</p><p><strong>Methods: </strong>This retrospective study included 133 primary OSCC samples, and the spatial pattern of Ki-67 in the tumor invasive zone was analyzed by immunohistochemistry (IHC). The prognostic value of tumor cells and stroma proliferative capacity in different POIs were assessed.</p><p><strong>Results: </strong>Ki-67 was widely expressed in tumor cells and stroma cells within the invasive zone, and cells in high-invasiveness POIs exhibit higher proliferation. Elevated Ki-67 expression in tumor cells was associated with poor overall survival (OS) and disease-free survival (DFS) in patients, which is independent of POIs. In our study, we identified the expression level of Ki-67 in tumor cells across high-invasiveness POIs as an independent risk factor for OS and DFS in OSCC patiens. Additionally, Ki-67 expression in surrounding non-tumor cells did not significantly correlate with patient survival.</p><p><strong>Conclusion: </strong>The remarkable proliferation characteristic of tumor cells in high-invasiveness POIs of OSCC tumors plays a crucial role in the prognosis of patients.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiologic Specific Growth Rate of Ameloblastomas: A Clinicopathological Correlation.","authors":"Chané Smit, Liam Robinson, Marlene B van Heerden, Pieter W Meyer, Felipe P Fonseca, Willie F P van Heerden, André Uys","doi":"10.1111/jop.13611","DOIUrl":"https://doi.org/10.1111/jop.13611","url":null,"abstract":"<p><strong>Background: </strong>The study aimed to assess the radiologic-specific growth rate of ameloblastomas, evaluating potential associations with demographics, radiologic features, histopathologic variants and proliferation indices. The results of this study will hopefully establish if any clinical or histopathologic features can elude fast-growing ameloblastomas.</p><p><strong>Methods: </strong>Patients presenting with multiple radiographs before surgical intervention due to various healthcare constraints or patient factors were included in the study. The measurements from each radiograph included the lesion's length, height, width and amount of expansion in these dimensions. Furthermore, the circumference of the lesion was measured in sagittal, coronal and axial planes. The radiologic-specific growth rate was assessed by calculating the difference in measurements from the initial to follow-up radiographs divided by the duration between the visits to calculate the growth rate per year.</p><p><strong>Results: </strong>The specific growth rate was analysed between age groups, histopathologic variants and Ki-67 values, with no statistically significant correlations found in all dimensions measured. A statistically significant faster growth (p = 0.04) was seen in females when measuring the mesial-distal length. When comparing radiologic features, ameloblastomas with loss of border demarcation, severe cortical destruction and tooth displacement demonstrated statistically significant faster growth.</p><p><strong>Conclusion: </strong>This study found significant correlations with the growth rate of ameloblastomas, specifically in coronal dimensions, supporting the notion of buccal-lingual growth/expansion for which ameloblastomas are known.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse Reactions to Cosmetic Fillers in the Oral and Maxillofacial Region: Clinico-Pathological, Histochemical, and Immunohistochemical Characterization.","authors":"Ana Cristina Tetzner, Laura Regina Mendes Viana, Lucas Guimarães Abreu, Elismauro Francisco Mendonça, Diego Antônio Costa Arantes, Ana Carolina Uchoa Vasconcelos, Ana Paula Neutzling Gomes, Cassiano Francisco Weege Nonaka, Pollianna Muniz Alves, Roberto Onner Cruz Tapia, Ricardo Alves Mesquita, Sílvia Ferreira de Sousa, Tarcília Aparecida Silva, Patrícia Carlos Caldeira","doi":"10.1111/jop.13604","DOIUrl":"https://doi.org/10.1111/jop.13604","url":null,"abstract":"<p><strong>Background: </strong>Cosmetic injections are increasing, as their complications, which can be misdiagnosed as neoplastic lesions. This study aimed to detail clinical, pathological, histochemical, and immunohistochemical features of adverse reactions to cosmetic fillers in the oral and maxillofacial region.</p><p><strong>Methods: </strong>Samples were retrieved from five pathology laboratories. Hematoxylin-eosin (H&E), Alcian Blue, Sirius Red, and Toluidine blue stains were performed, as well as immunohistochemistry for CD68, CD3, and CD20. H&E was evaluated under polarization. Descriptive statistics were performed.</p><p><strong>Results: </strong>Twenty-three cases were included. Polymethyl-methacrylate was the most common material. Most reactions affected women, lips and were asymptomatic, with a variable time of evolution, presenting as nodules. Materials had different shape and size on H&E. Giant cells were commonly found, except in silicone and hyaluronic acid. Foreign-body granuloma was frequent in polymethyl-methacrylate. Calcium hydroxyapatite and poly-L-lactic acid were refractile under polarized light. Hyaluronic acid and polyacrylamide hydrogel were metachromatic by Toluidine blue. Alcian blue was positive in all cases of hyaluronic acid. Mast cells were detected in all materials, except hyaluronic acid and polyacrylamide hydrogel. Eosinophils were rarer than mast cells. Numerous CD68-positive cells were seen in all cases. All cases had CD3-positive cells, with variable amounts. CD20 was scant or negative in most cases.</p><p><strong>Conclusions: </strong>An evident macrophage reaction is observed in all aesthetic fillers, frequently associated with giant cell formation. Despite similarities, there are specific features of each material and the host response that assist the correct histopathological diagnosis. Immunohistochemistry for CD68 and Toluidine blue stain are useful in doubtful cases.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers.","authors":"Katarina Zeljic, Dunja Pavlovic, Goran Stojkovic, Sandra Dragicevic, Jelena Ljubicic, Nikola Todorovic, Aleksandra Nikolic","doi":"10.1111/jop.13606","DOIUrl":"https://doi.org/10.1111/jop.13606","url":null,"abstract":"<p><strong>Introduction: </strong>A recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes TNS3 and LRRFIP1 in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211.</p><p><strong>Objective: </strong>Our aim was to investigate the biomarker potential of TNS3-203 and LRRFIP1-211 transcripts in oral cancer, the most common type of head and neck cancer.</p><p><strong>Materials and methods: </strong>An in silico approach was used to characterize the promoters and transcripts of interest. Relative expression of TNS3-203 and LRRFIP1-211 transcripts was evaluated by qRT-PCR in a group of 46 oral cancer patients in samples of cancer and adjacent non-cancerous tissue.</p><p><strong>Results: </strong>TNS3-203 was significantly overexpressed in oral cancer compared with matched non-cancerous tissue, so this transcript can potentially be used as a diagnostic biomarker. There were no differences in LRRFIP1-211 level between analyzed tissues. None of the investigated transcripts has prognostic potential in oral cancer.</p><p><strong>Conclusion: </strong>The results obtained indicate the diagnostic potential of TNS3-203, but not LRRFIP1-211 transcript and its role in oral carcinogenesis.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boswellia Extract Promotes Healing and Resolving Inflammation in Oral Ulcers of Rat.","authors":"Wei Zhao, Zhuoqun Jia, Jiao Han, Xiaojun Sun","doi":"10.1111/jop.13609","DOIUrl":"https://doi.org/10.1111/jop.13609","url":null,"abstract":"<p><strong>Background: </strong>Recurrent aphthous ulcers significantly impact patients' quality of life due to their painful and recurrent nature, necessitating more effective treatments. This study explores the therapeutic potential of Boswellia to treat recurrent aphthous ulcers by its anti-inflammatory and healing promotion effect in a rat oral ulcer model.</p><p><strong>Methods: </strong>Network pharmacology techniques were employed to elucidate Boswellia's active components and potential targets. Intersecting targets of Boswellia and oral ulcer-related genes were screened for protein-protein interaction network analysis and functional enrichment. An oral ulcer model in rats was used and rats were treated with Boswellia extract. The healing process was monitored by measuring the ulcer area and body weight changes. Histological analysis was performed, and the role of Boswellia in macrophage polarization was investigated through gene expression analysis and protein array tests. The underlying mechanism involving PPARγ activation was also explored.</p><p><strong>Results: </strong>Network pharmacology analysis revealed Boswellia's interaction with key genes and pathways associated with inflammation and lipid metabolism, such as MAPK3, PPARG, and PTGS2. Boswellia extract significantly accelerated oral ulcer healing and recovered weight loss in rats. Histological examinations revealed reduced tissue swelling and inflammatory cell infiltration in treated groups. Furthermore, Boswellia extract decreased infiltration of M1 macrophage presence while increasing M2 macrophage, indicating an inflammation-resolving effect. In vitro studies showed that Boswellia extract enhanced M2-related gene expression and decreased pro-inflammatory cytokines, which is PPARγ dependent.</p><p><strong>Conclusion: </strong>Boswellia extract promotes oral ulcer healing and resolves inflammation, primarily through the modulation of macrophage polarization via PPARγ activation.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PETRICCS Guideline Protocol: A Call to Improve Reporting Standards in Cell Culture Research.","authors":"Mylene Martins Monteiro, Juliana Amorim Dos Santos, Victor Paiva Barbosa, Graziela De Luca Canto, Cristiane H Squarize, Ricardo D Coletta, Eliete Neves Silva Guerra","doi":"10.1111/jop.13605","DOIUrl":"https://doi.org/10.1111/jop.13605","url":null,"abstract":"<p><strong>Background: </strong>Cell culture studies play an important role in addressing fundamental scientific questions. However, inadequate reporting of these studies results in a lack of transparency and reproducibility. Recognizing the need for improvement, several ongoing efforts, such as CRIS guidelines and the ICLAC checklist, are focused on enhancing best practices for in vitro studies. Nonetheless, a comprehensive guideline specifically addressing the reporting of cell culture methods remains lacking. In this manner, a consensus-based approach is being undertaken to develop the Preferred and Transparent Reporting Items for Cell Culture Studies (PETRICCS) guideline. This project aims to present the protocol and the details for its development.</p><p><strong>Methods: </strong>The process comprises five phases: (i) Initial Steps: a Steering Committee identifies the need for a guideline and drafts the PETRICCS protocol; (ii) Pre-meeting: an International Group of Cell Culture Experts (IGCE) reviews the draft guideline through a Delphi consensus exercise; (iii) Consensus Meeting: the steering committee presents the guideline's development, addresses concerns, and reaches consensus on the final items; (iv) Post-meeting: explanatory documents are prepared to assist authors in reporting their findings; and (v) Post-publication: PETRICCS, along with supporting documents, is published and made freely accessible.</p><p><strong>Results and conclusions: </strong>PETRICCS will assist researchers in reporting and reviewing cell culture findings, enhancing transparency and reproducibility while filling a gap in this crucial scientific field. The guideline will incorporate the experiences of experts, creating a more equitable environment for authors, peer-reviewers, and editors during the publication process.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of 4NQO- and Ethanol-Induced Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma in Male and Female Mice: Association With Peripheral Blood Inflammatory Markers.","authors":"Anaíra Ribeiro Guedes Fonseca Costa, Débora de Oliveira Santos, Isabella Moura Pereira, Rafael Borges Rosa, Emilene Ferreira de Castro, Ianca Daniele Oliveira de Jesus, Mariana Daiani Costa Silva, Sérgio Vitorino Cardoso, Adriano Mota Loyola, Paulo Rogério de Faria","doi":"10.1111/jop.13602","DOIUrl":"https://doi.org/10.1111/jop.13602","url":null,"abstract":"<p><strong>Background: </strong>Considering that peripheral blood biomarkers are prognostic predictors for several human tumors, this study aimed to comparatively analyze the association of hematological alterations with the incidence of epithelial dysplasia (ED) and oral squamous cell carcinoma (OSCC) in male and female mice treated with 4-nitroquinoline-N-oxide (4NQO) and ethanol (EtOH).</p><p><strong>Methods: </strong>120 C57Bl/6J mice (60 males and 60 females) were allocated to four groups (n = 15). They were treated firstly either with 5 mg/mL propylene glycol (PPG) or 100 μg/mL 4NQO in the drinking water for 10 weeks, followed by sterilized water (H₂O) or 8% EtOH (v/v) for 15 weeks, as follows: PPG/H₂O, PPG/EtOH, 4NQO/H₂O, and 4NQO/EtOH (CEUA-UFU, #020/21). After killing, tongues were collected for histopathological analysis of ED and OSCC. Blood samples were processed for complete blood count and calculation of neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII).</p><p><strong>Results: </strong>The incidence of OSCC in females from the 4NQO/EtOH group (60%) was lower when compared to males (93%). Neutrophils, NLR, and SII increased from control animals (PPG/H₂O and PPG/EtOH) to ED and OSCC-bearing male and female mice (4NQO/H₂O and 4NQO/EtOH), while lymphocytes decreased. Females from the 4NQO/H₂O group with ED also had higher neutrophils, NRL, and SII values than females with normal tongues.</p><p><strong>Conclusion: </strong>The low incidence of 4NQO- and ethanol-induced OSCC in females indicates that the sex bias in OSCC may not be associated with extrinsic risk factors alone. Neutrophil and lymphocyte counts, NRL, and SII were significantly altered during multistep carcinogenesis and thus could be explored as biomarkers for ED and OSCC development.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL5 Promotes Tumor Progression in Oral Squamous Cell Carcinoma by Activating the Myc Pathway.","authors":"Rong Huang, Xingyu Feng, Tao Zhou, Jiajing Lu, Ying Wang, Shuangyue Zhang, Wei Zhang","doi":"10.1111/jop.13601","DOIUrl":"https://doi.org/10.1111/jop.13601","url":null,"abstract":"<p><strong>Background: </strong>It has been observed that methyltransferases-like 5 (METTL5) is a key mediator underlying tumorigenesis in humans. This study aimed to investigate the biological function, expression pattern, and clinical significance of METTL5 in oral squamous cell carcinoma (OSCC).</p><p><strong>Methods: </strong>Bioinformatics interrogations and immunohistochemical staining were utilized for determining the expression and localization of METTL5 in OSCC, and revealing the relationship between the expression of METTL5 and prognosis. Cell phenotype experiments and xenograft models were used to detect the impact of METTL5 silencing on OSCC. Gene Set Enrichment Analysis, Spearman correlation, and c-Myc overexpression plasmid was utilized for exploring the regulatory effects of METTL5 on the Myc pathway.</p><p><strong>Results: </strong>METTL5 was overexpressed in OSCC and correlated with reduced survival. Cell proliferation, migration, and invasion were significantly inhibited by METTL5 knockdown in vitro, and tumor growth was impaired in vivo. Moreover, METTL5 was capable of activating the Myc pathway. The influences of knockdown of METTL5 on Cal27 cell biological behaviors were reversed by overexpression of c-Myc.</p><p><strong>Conclusions: </strong>Our data suggested that METTL5 may act as a putative oncogene and prognostic biomarker in OSCC. The Myc pathway appears to be involved in cell proliferation, migration, invasion, and apoptosis in OSCC mediated by METTL5.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Cell Cycle Regulatory Proteins p16, CDK4, and PTEN Are Unsuitable as Biomarkers in Oral Melanocytic Lesions.","authors":"Aline Queiroz, Thalita Santana, Adriana Fraga Costa Paris, Marília Trierveiler","doi":"10.1111/jop.13607","DOIUrl":"https://doi.org/10.1111/jop.13607","url":null,"abstract":"<p><strong>Background: </strong>Melanocytic neoplasms are rare in the oral cavity and represent a diagnostic challenge due to the overlap between benign and malignant lesions. However, their pathogenesis is not fully elucidated. The aim of this study was to evaluate the expression of the cell cycle-related proteins p16, CDK4, and PTEN in oral melanocytic nevi and melanomas.</p><p><strong>Methods: </strong>A total of 42 cases of melanocytic nevi and five cases of melanoma underwent immunohistochemical analysis. Cases were scored as 0, 1 (< 5% of positive cells), 2 (6%-50% of positive cells), and 3 (> 50% of positive cells). Statistical significance was set at p ≤ 0.05.</p><p><strong>Results: </strong>Two cases of melanocytic nevi were totally negative for p16 and 95.2% of cases scored 1. For CDK4, 47.6% of the cases scored 2 and 52.4% scored 3. For PTEN, 97.6% of the cases scored 3 and only one case showed a score of 2. All melanoma cases were classified with a score of 2 for p16, and for CDK4 and PTEN, all cases exhibited a score of 3. PTEN and CDK4 were higher expressed when compared to p16 both in melanocytic nevi and melanomas (p < 0.001), and melanocytic nevi showed low expression of p16 when compared to melanomas (p < 0.0001).</p><p><strong>Conclusion: </strong>The findings suggest that these cell cycle-related proteins are not useful biomarkers in melanocytic lesions of the oral mucosa and support the apparent biological distinction between oral and cutaneous lesions.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}