Panax notoginseng Saponins Alleviate OSF by Inhibiting Ferroptosis Through GPX4 Activation.

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-10-09 DOI:10.1111/jop.70069

Xinyue Zhang, Yujie Sun, Chenxi Zhao, Hong Zou, Liang Hu, Lixiang Wen, Qun Tang

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引用次数: 0

Abstract

Objectives: Oral submucous fibrosis (OSF) is a chronic progressive disease characterized by fibrosis. Panax notoginseng saponins (PNS) are effective components of the traditional Chinese medicine Panax notoginseng and play an important role in the treatment of OSF. However, the underlying mechanism of action for this medicine remains unclear. The aim of this study was to explore the effects of ferroptosis in OSF.

Subjects and methods: The mice were divided into six groups. Mouth openings were detected. The buccal tissues were harvested for histomorphological analysis, western blot, and RT-qPCR. The cells were harvested for CCK8 detection, western blot, RT-qPCR, TEM, immunofluorescence, fluorescent probe, and colorimetry.

Results: PNS improved mouth opening, alleviated tissue cell damage, and inhibited ferroptosis and fibrosis in OSF rat models. Our study found that ferroptosis is closely related to arecoline-induced OSF and that PNS inhibits ferroptosis by upregulating glutathione peroxidase 4 (GPX4) to alleviate OSF.

Conclusions: The research established OSF in vivo and in vitro, respectively, and the changes in the expression of fibrosis and ferroptosis-related markers in these models were studied to provide a theoretical and experimental basis for the clinical prevention and treatment of OSF.

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三七总皂苷通过GPX4激活抑制铁下垂减轻OSF。

目的：口腔黏膜下纤维化（OSF）是一种以纤维化为特征的慢性进行性疾病。三七皂苷（PNS）是中药三七的有效成分，在治疗OSF中起重要作用。然而，这种药物的潜在作用机制尚不清楚。本研究的目的是探讨铁下垂对OSF的影响。实验对象和方法：将小鼠分为6组。检测到开口。采集口腔组织进行组织形态学分析、western blot和RT-qPCR。收集细胞进行CCK8检测、western blot、RT-qPCR、TEM、免疫荧光、荧光探针和比色法检测。结果：PNS改善OSF大鼠模型的开口，减轻组织细胞损伤，抑制铁下垂和纤维化。我们的研究发现，铁下垂与茴香碱诱导的OSF密切相关，PNS通过上调谷胱甘肽过氧化物酶4 （glutathione peroxidase 4, GPX4）来抑制铁下垂，减轻OSF。结论：本研究分别在体内和体外建立了OSF，研究了这些模型中纤维化和凋亡相关标志物的表达变化，为临床防治OSF提供了理论和实验依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.