The Cell Cycle Regulatory Proteins p16, CDK4, and PTEN Are Unsuitable as Biomarkers in Oral Melanocytic Lesions

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-01-27 DOI:10.1111/jop.13607

Aline Queiroz, Thalita Santana, Adriana Fraga Costa Paris, Marília Trierveiler

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引用次数: 0

Abstract

Background

Melanocytic neoplasms are rare in the oral cavity and represent a diagnostic challenge due to the overlap between benign and malignant lesions. However, their pathogenesis is not fully elucidated. The aim of this study was to evaluate the expression of the cell cycle-related proteins p16, CDK4, and PTEN in oral melanocytic nevi and melanomas.

Methods

A total of 42 cases of melanocytic nevi and five cases of melanoma underwent immunohistochemical analysis. Cases were scored as 0, 1 (< 5% of positive cells), 2 (6%–50% of positive cells), and 3 (> 50% of positive cells). Statistical significance was set at p ≤ 0.05.

Results

Two cases of melanocytic nevi were totally negative for p16 and 95.2% of cases scored 1. For CDK4, 47.6% of the cases scored 2 and 52.4% scored 3. For PTEN, 97.6% of the cases scored 3 and only one case showed a score of 2. All melanoma cases were classified with a score of 2 for p16, and for CDK4 and PTEN, all cases exhibited a score of 3. PTEN and CDK4 were higher expressed when compared to p16 both in melanocytic nevi and melanomas (p < 0.001), and melanocytic nevi showed low expression of p16 when compared to melanomas (p < 0.0001).

Conclusion

The findings suggest that these cell cycle-related proteins are not useful biomarkers in melanocytic lesions of the oral mucosa and support the apparent biological distinction between oral and cutaneous lesions.

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细胞周期调控蛋白 p16、CDK4 和 PTEN 不适合作为口腔黑色素细胞病变的生物标记物。

背景：黑色素细胞肿瘤在口腔中是罕见的，由于良性和恶性病变之间的重叠，代表了诊断的挑战。然而，其发病机制尚未完全阐明。本研究的目的是评估细胞周期相关蛋白p16、CDK4和PTEN在口腔黑色素细胞痣和黑色素瘤中的表达。方法：对42例黑色素细胞痣和5例黑色素瘤进行免疫组化分析。病例评分为0,1（50%阳性细胞）。p≤0.05为差异有统计学意义。结果：2例黑素细胞痣p16完全阴性，95.2%为1分。CDK4得分为2分的占47.6%，得分为3分的占52.4%。PTEN评分为3分的占97.6%，2分的仅有1例。所有黑色素瘤病例的p16评分为2分，CDK4和PTEN评分为3分。与p16相比，PTEN和CDK4在黑素细胞痣和黑素瘤中的表达更高(p结论：研究结果表明，这些细胞周期相关蛋白在口腔黏膜黑素细胞病变中不是有用的生物标志物，并支持口腔和皮肤病变之间明显的生物学区别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.