The Cell Cycle Regulatory Proteins p16, CDK4, and PTEN Are Unsuitable as Biomarkers in Oral Melanocytic Lesions

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-01-27 DOI:10.1111/jop.13607

Aline Queiroz, Thalita Santana, Adriana Fraga Costa Paris, Marília Trierveiler

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引用次数: 0

Abstract

Background

Melanocytic neoplasms are rare in the oral cavity and represent a diagnostic challenge due to the overlap between benign and malignant lesions. However, their pathogenesis is not fully elucidated. The aim of this study was to evaluate the expression of the cell cycle-related proteins p16, CDK4, and PTEN in oral melanocytic nevi and melanomas.

Methods

A total of 42 cases of melanocytic nevi and five cases of melanoma underwent immunohistochemical analysis. Cases were scored as 0, 1 (< 5% of positive cells), 2 (6%–50% of positive cells), and 3 (> 50% of positive cells). Statistical significance was set at p ≤ 0.05.

Results

Two cases of melanocytic nevi were totally negative for p16 and 95.2% of cases scored 1. For CDK4, 47.6% of the cases scored 2 and 52.4% scored 3. For PTEN, 97.6% of the cases scored 3 and only one case showed a score of 2. All melanoma cases were classified with a score of 2 for p16, and for CDK4 and PTEN, all cases exhibited a score of 3. PTEN and CDK4 were higher expressed when compared to p16 both in melanocytic nevi and melanomas (p < 0.001), and melanocytic nevi showed low expression of p16 when compared to melanomas (p < 0.0001).

Conclusion

The findings suggest that these cell cycle-related proteins are not useful biomarkers in melanocytic lesions of the oral mucosa and support the apparent biological distinction between oral and cutaneous lesions.

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细胞周期调控蛋白 p16、CDK4 和 PTEN 不适合作为口腔黑色素细胞病变的生物标记物。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.