Journal of Oral Pathology & Medicine最新文献

{"title":"A Topical Combination of Xyloglucan and Pea Protein Effectively Manages Oral Ulcers In Vivo.","authors":"Alessia Filippone, Giovanna Casili, Marika Lanza, Michela Campolo, Irene Paterniti, Laura Cucinotta, Rossella Basilotta, Alessio Ardizzone, Emanuela Esposito","doi":"10.1111/jop.70029","DOIUrl":"https://doi.org/10.1111/jop.70029","url":null,"abstract":"<p><strong>Background: </strong>Aphthous stomatitis (AS) is a common idiopathic condition that affects approximately 5% to 25% of the general population. AS is characterized by recurrent painful aphthous ulcers on non-keratinized oral mucous membranes. Lifestyle modifications and topical treatments are often used to manage symptoms, but no definitive cure exists. This study aims to evaluate the ability of a topical gel containing xyloglucan and pea protein (TXP), two mucoprotective substances, to restore the integrity of oral epithelial cells and manage inflammation associated with AS.</p><p><strong>Methods: </strong>In this study, oral ulcers were induced in rats by applying a phenol solution for 60 s to the mucosa of the left cheek. Following the formation of the lesion (24 h later) topical treatment with TXP was carried out for 2, 4, and 5 days. The healing properties of TXP were assessed by histopathological examination of the oral mucosa through the evaluation of morphological criteria such as hyperemia, edema, and presence of neutrophilic infiltration and by measuring the expression levels of inflammatory markers such as TNF-α and IL-2.</p><p><strong>Results: </strong>Results demonstrated that TXP was able to effectively treat oral ulcers by reducing the degree of hyperemia and edema in cheek tissue already at day 4. Moreover, TXP significantly decreased the levels of IL-2 and TNF-α after 4 days of gel application.</p><p><strong>Conclusions: </strong>This study demonstrates that TXP possesses a significant ulcer repairing ability in vivo, as it effectively restores the integrity of oral mucosal tissue, thus helping to reduce inflammation.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Tissue Staining With 1% Methylene Blue Solution After Enucleation of Odontogenic Keratocyst","authors":"Gustavo Henrique Martins,&nbsp;José Alcides Almeida de Arruda,&nbsp;Bruno Augusto Benevenuto de Andrade,&nbsp;Rayssa Nunes Villafort,&nbsp;Marcio Bruno Figueiredo do Amaral,&nbsp;Felipe Paiva Fonseca,&nbsp;Lucas Guimarães Abreu,&nbsp;Ricardo Alves Mesquita","doi":"10.1111/jop.70031","DOIUrl":"10.1111/jop.70031","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Odontogenic keratocyst (OKC) treatment often involves enucleation followed by peripheral ostectomy to reduce recurrence. However, identifying bone areas containing epithelial remnants remains a surgical challenge. The intraoperative use of 1% methylene blue solution has been proposed to delineate residual epithelial tissue. This study aimed to evaluate the presence of epithelial structures in both OKC surgical specimens and bone tissue stained with 1% methylene blue solution following enucleation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective analysis was conducted on histological records from OKC cases treated between August 2022 and December 2023. The primary variable was the presence of epithelial structures, including daughter cysts, microcysts, and epithelial remnants. Data were analyzed descriptively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twelve patients (mean age: 40.8 years) were included. Most lesions were located in the posterior mandible (50%) and exhibited a unilocular radiographic pattern (91.7%), with a mean lesion size of 25 mm. Epithelial structures were identified in 75% of OKC surgical specimens and in 16.7% of the stained bone tissues. The mean follow-up period was 26.5 months, with no recurrences observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of 1% methylene blue solution may aid in the intraoperative identification of residual epithelial tissue, potentially contributing to more precise and conservative OKC management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"770-776"},"PeriodicalIF":2.3,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Expressed NELFE in Tumor Cells at the Invasive Tumor Front Was a Prognostic Biomarker for Oral Squamous Cell Carcinoma","authors":"Yizhuo Xue,&nbsp;Lingyun Liu,&nbsp;Liyuan Yu,&nbsp;Zihui Li,&nbsp;Xiaofeng Huang,&nbsp;Sheng Chen,&nbsp;Yue Jing,&nbsp;Xiaoxin Zhang,&nbsp;Liang Ding,&nbsp;Yuxian Song,&nbsp;Zhiyong Wang,&nbsp;Yanhong Ni","doi":"10.1111/jop.70025","DOIUrl":"10.1111/jop.70025","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Negative elongation factor E (NELFE) is a crucial subunit of the NELF complex, which plays pivotal roles in transcriptional regulation and cell fate. Despite the role of dysregulated NELFE in promoting the onset and progression of some kinds of tumors, its expression profile, prognostic value, and function in oral squamous cell carcinoma (OSCC) still remain unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Immunohistochemistry (IHC) was utilized to analyze the NELFE expression profile in OSCC, and the prognostic values of NELFE were also evaluated. qRT-PCR and Western blot were used to compare the expression of NELFE in OSCC cell lines. NELFE expression was knocked down by small interfering RNA fragments, and functional experiments were conducted to investigate the impact of NELFE on the proliferation and migration of OSCC cell lines. Gene set enrichment analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data to explore potential mechanisms of NELFE in OSCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NELFE was widely distributed throughout tumor cells (TCs), fibroblast-like cells (FLCs), and tumor infiltrating lymphocytes (TILs) in OSCC tissue. However, its expression was significantly upregulated in TCs at the invasive tumor front compared to the tumor center components (<i>p</i> = 0.027). Higher expression of NELFE in TCs at the invasive tumor front (ITF) was significantly associated with higher lymph node metastasis (<i>p</i> = 0.024), worse invasion pattern (<i>p</i> &lt; 0.0001), and more advanced tumor node metastasis classification (<i>p</i> = 0.002). Additionally, higher expression of NELFE in TCs at the invasive tumor front was also linked to higher recurrence (<i>p</i> = 0.021) and shorter disease-free (HR = 2.045, <i>p</i> = 0.006) as well as relapse-free survival (HR = 2.369, <i>p</i> = 0.011). The NELFE mRNA and protein were generally expressed in OSCC cells. Functionally, silencing NELFE significantly inhibited the proliferation and migration capabilities of OSCC tumor cells in vitro. Finally, GSEA revealed a significant difference in the E2F pathway between NELFE high and low expression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>NELFE expression at the invasive front was associated with poor prognosis and could be a potential prognostic biomarker for OSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"757-769"},"PeriodicalIF":2.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concordance of Oral and Anal Human Papillomavirus in Men Who Have Sex With Men Living With Human Immunodeficiency Virus","authors":"Po-Chi Huang,&nbsp;Szu-I Lin,&nbsp;Na-Lee Sun,&nbsp;Shu-Yuan Li,&nbsp;Cheng-Pin Chen,&nbsp;Yi-Chun Lin,&nbsp;Chien-Yu Cheng,&nbsp;Fang-Yeh Chu,&nbsp;Shu-Hsing Cheng","doi":"10.1111/jop.70023","DOIUrl":"10.1111/jop.70023","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>To detect human papillomavirus (HPV) at oral and anal sites and assess genotype concordance among men who have sex with men (MSM) living with human immunodeficiency virus (HIV) in Taiwan.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>HIV-positive men aged 20 years or older attending outpatient clinics at Taoyuan General Hospital, Taiwan, were enrolled. Oral gargle and anal swab samples were collected for HPV genotyping using linear array testing. Multivariate logistic regression identified factors associated with oral-HPV and oral-anal genotype concordance. Significance was set at <i>p</i> &lt; 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 196 eligible participants, the mean age was 32.2 years and over 95% were MSM. The mean CD4+ T cell count was 588 cells/μL (95% confidence interval [CI]: 551–626), and 73% (<i>n</i> = 143) had suppressed viral loads. Anal-HPV was detected in 184 participants (93.8%, 95% CI: 90.5%–97.2%), with 82.7% (<i>n</i> = 162) having oncogenic genotypes. Oral HPV was found in 20 participants (10.2%, 95% CI: 5.9%–14.4%), with 30% (<i>n</i> = 6) having oncogenic genotypes. Among those with oral HPV, 55% (<i>n</i> = 11) showed genotype concordance with anal sites. Only 30% reported condom use during oral sex. Concordance was significantly associated with the number of anal-HPV genotypes (adjusted odds ratio: 1.58, 95% CI: 1.08–2.32, <i>p</i> = 0.015), adjusting for drug use, internet-based partners, recent sexually transmitted infections, and anal oncogenic HPV count.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Oral HPV was common, and over half of the infections matched genotypes found anally. These results support targeted HPV vaccination promotion among MSM living with HIV in Taiwan.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"746-756"},"PeriodicalIF":2.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein–Barr Virus Serology Associated With Persistent Oral Human Papillomavirus Infections in Men","authors":"Sanni Rinne,&nbsp;Birgitta Michels,&nbsp;Julia Butt,&nbsp;Kari Syrjänen,&nbsp;Seija Grenman,&nbsp;Tim Waterboer,&nbsp;Stina Syrjänen,&nbsp;Karolina Louvanto","doi":"10.1111/jop.70015","DOIUrl":"10.1111/jop.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Most people acquire Epstein–Barr virus (EBV) and certain human papillomaviruses (HPVs) during their lifetime. HPV-related oropharyngeal carcinomas have increased in recent decades, particularly among men. The role of coinfection with viruses like EBV on HPV outcomes is unclear. We investigated potential associations between EBV serology and longitudinal outcomes of oral HPV infections in men.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study included 119 men from the Finnish Family HPV Study who were followed up for 3 years. Blood and oral cavity samples were collected at baseline, 12-, 24-, and 36-month follow-up visits. HPV was genotyped with the Multimetrix assay, and the serum IgG antibodies of EBV proteins Zebra, EA-D, EBNA, and VCAp18 were measured with fluorescent bead-based multiplex serology. Univariate regression analysis was used to measure the strength of the association between different variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Most participants (99.2%; <i>n</i> = 118) were EBV-seropositive with stable antibody titers throughout the follow-up. Self-reported history of atopy was positively associated with elevated EBNA-1 levels, with OR 7.43 (95% CI: 1.39–39.76). EBV seropositivity with high titers and elevated EA-D levels alone increased the risk of type-specific oral HPV persistence for Types 16, 18, 33, and 51, with OR 4.20 (95% CI: 1.09–16.19) and OR 6.23 (95% CI: 1.19–32.75), respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Most of the participants were EBV-seropositive as expected. Elevated EA-D antibody levels and being EBV-seropositive with high titers significantly increased the risk of type-specific oral HPV persistence among these men.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"733-741"},"PeriodicalIF":2.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of PDGFRA Gene Pathogenic Variants in Nasal Polyps Associated With Chronic Rhinosinusitis","authors":"Tamara da Silva Vieira,&nbsp;Letícia Martins Guimarães,&nbsp;Marina Gonçalves Diniz,&nbsp;Wilma Terezinha Anselmo-Lima,&nbsp;Edwin Tamashiro,&nbsp;Luiz Armando De Marco,&nbsp;Fabiana Cardoso Pereira Valera,&nbsp;Carolina Cavalieri Gomes","doi":"10.1111/jop.70027","DOIUrl":"10.1111/jop.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chronic rhinosinusitis (CRS) with nasal polyps is an inflammatory condition of the nasal mucosa accompanied by significant tissue remodeling. It is characterized by a type 2 inflammatory response and epithelial barrier dysfunction. Platelet-derived growth factor receptor alpha (<i>PDGFRA</i>) is a proto-oncogene that encodes a receptor tyrosine kinase for which platelet-derived growth factor-A (<i>PDGFA</i>) is a specific ligand. Immunoexpression of <i>PDGFRA</i> and upregulation of <i>PDGFRA</i> and <i>PDGFA</i> mRNAs have been demonstrated in CRS with nasal polyps, suggesting they participate in the pathophysiology of such lesions. <i>PDGFRA</i> pathogenic variants have been reported in inflammatory fibroid polyps, as well as in gastrointestinal stromal tumors. Although the pathogenesis of CRS with nasal polyps has been extensively studied, it remains unclear whether nasal polyps harbor genetic mutations. Therefore, herein we investigated the presence of <i>PDGFRA</i> pathogenic variants in nasal polyps occurring in the context of CRS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Fourteen CRS with nasal polyp samples were Sanger sequenced, targeting <i>PDGFRA</i> exons 12 and 18.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All samples exhibited wild-type sequences for the <i>PDGFRA</i> sequenced regions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings suggest that, unlike inflammatory fibroid polyps occurring in the gastrointestinal tract, the pathogenesis of nasal polyps occurring in CRS does not involve <i>PDGFRA</i> mutations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"742-745"},"PeriodicalIF":2.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrimethamine Triggers the Apoptotic Pathway in Mucoepidermoid Carcinoma in Cell-Based Models","authors":"Hyun-Ji Kim,&nbsp;Dong-Guk Park,&nbsp;Su-Jung Choi,&nbsp;Jae-Jin Cho,&nbsp;Seong-Doo Hong,&nbsp;Sung-Dae Cho","doi":"10.1111/jop.70024","DOIUrl":"10.1111/jop.70024","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Mucoepidermoid carcinoma (MEC) is the most prevalent salivary gland malignancy, with a poor prognosis in high-grade tumors at diagnosis. This highlights the need for effective antitumor agents for treating MEC. Therefore, we aimed to investigate the antineoplastic efficacy of pyrimethamine (PYR), a Food and Drug Administration-approved antiparasitic medicine, to repurpose it as an alternative therapeutic option for treating human MEC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The trypan blue exclusion assay, cell counting kit-8 assay, and live/dead assay were performed to assess the antiproliferative efficacy of PYR. PYR-induced apoptosis was confirmed with 4′,6-diamidino-2-phenylindole staining, cell cycle analysis, and annexin V/propidium iodide staining. A western blot assay was conducted to measure changes in cleaved caspase 8 and myeloid cell leukemia-1 (Mcl-1) expression after PYR treatment. Mcl-1 overexpression was used to further confirm the apoptosis-inducing activity of PYR. The hanging drop method was employed to assess the efficacy of PYR in a three-dimensional culture system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PYR-induced apoptotic cell death in the YD-15 high-grade MEC cell line by promoting apoptosis, as evidenced by MCl-1 proteasomal degradation and increased cleaved caspase 8 expression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our results indicate that PYR can effectively target human MEC by inducing both intrinsic and extrinsic apoptotic pathways.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"723-732"},"PeriodicalIF":2.3,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Expression of H3K9ac and H3K27ac in Premalignant and Malignant Tongue Lesions of Wild-Type and Nos2-Knockout Mice Treated With 4NQO","authors":"Anaíra Ribeiro Guedes Fonseca Costa,&nbsp;Débora de Oliveira de Santos,&nbsp;Mariana Daiani Costa Silva,&nbsp;Ianca Daniele Oliveira de Jesus,&nbsp;Lúbia Cristina Fonseca,&nbsp;Sérgio Vitorino Cardoso,&nbsp;Paulo Rogério de Faria,&nbsp;Adriano Mota Loyola","doi":"10.1111/jop.70021","DOIUrl":"10.1111/jop.70021","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Nitric oxide is an important regulator of the epigenetic landscape of cellular homeostatic and pathological states, in which post-translational modifications of the epigenome-modulating enzymes are the most well described mechanism. Considering that alterations of the histone acetylation pattern were associated with oral cancer development and progression, the purpose of this study was to analyze the immunohistochemical expression of H3K9ac and H3K27ac at different stages of oral carcinogenesis induced by 4-nitroquinoline-<i>N</i>-oxide (4NQO) in Nos2^+/+ (wild-type) and Nos2^−/− (knockout) mice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>C57BL/6J and B6.129P2-Nos2^tm1Lau/J mice were treated with 4NQO in the drinking water at 50 μg/mL for 16 weeks and observed for 8 weeks. Tongues were submitted to histopathological analysis and immunohistochemistry for H3K9ac and H3K27ac expression. The antigen–antibody reaction was analyzed with quickscore (QS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Both histone acetylation marks were expressed in the normal epithelium. QS values were higher in moderate dysplasia of Nos2^−/− mice (<i>p</i> = 0.025) when compared to Nos2^+/+, and mild dysplasia had lower values for H3K9ac when compared to moderate and severe dysplasia in the Nos2^−/− group (<i>p</i> = 0.015). H3K27ac significantly increased from normal mucosa to mild dysplasia in Nos2^+/+ mice (<i>p</i> = 0.007). Additionally, Nos2^+/+ mice had a higher number of H3K27ac-positive mild dysplasias when compared to Nos2^−/− (<i>p =</i> 0.023).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The pattern of histone acetylation changes in murine oral carcinogenesis, mainly when the epithelial lining of the tongue becomes dysplastic, and such epigenetic modifications might be iNOS-mediated.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"715-722"},"PeriodicalIF":2.3,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Unique Plasma Protein Signature Characterizes Squamous Cell Carcinoma of the Oral Tongue in Young Adults","authors":"Xiaolian Gu,&nbsp;Philip J. Coates,&nbsp;Lixiao Wang,&nbsp;Sivakumar Vadivel Gnanasundram,&nbsp;Nicola Sgaramella,&nbsp;Nima Attaran,&nbsp;Baris Erdogan,&nbsp;Mustafa Magan,&nbsp;Karin Nylander","doi":"10.1111/jop.70020","DOIUrl":"10.1111/jop.70020","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The incidence of squamous cell carcinoma of the oral tongue (SCCOT) among young adults is increasing in several regions of the world. Age-dependent differences in the biology of SCCOT have been suspected.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We used the Olink Explore 3072 high-throughput platform to comprehensively quantify plasma proteins in 24 young (≤ 40 years of age) and 50 old (&gt; 50 years of age) individuals. Eight young and 20 old individuals were diagnosed with SCCOT, four young and nine old individuals with SCC at other oral subsites (SCCOO), and the remaining 12 young and 21 old individuals were healthy controls. Dimension reduction analysis, differential expression analysis, and functional enrichment analysis were performed to characterize young patient-specific biological signatures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Plasma levels of 2923 proteins were obtained. Principal component analysis indicated age-related expression patterns. Comparing young patients to young controls/old patients/old controls, differential abundance analysis showed that increases in protein levels of Peroxiredoxin 2 (PRDX2) and C-C motif chemokine ligand 26 (CCL26) and a decrease in Kallikrein related peptidase 4 (KLK4) were young patient-specific. Reactome pathway enrichment analysis identified “Cellular response to chemical stress,” “Detoxification of reactive oxygen species” and “Cellular responses to stimuli” as the top altered pathways in young patients with SCCOT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Abnormal cellular stress and aberrant immune regulation could thus be linked to cancer development in young patients. The unique plasma proteomic signature observed in young patients with SCCOT suggests that they constitute a specific group with distinct underlying pathophysiological processes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"706-714"},"PeriodicalIF":2.3,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological Study of Oncocytomas of Head and Neck Region: A Systematic Review","authors":"João Paulo Gonçalves de Paiva,&nbsp;Laura Borges Kirschnick,&nbsp;Daniela Giraldo Roldán,&nbsp;Manoela Domingues Martins,&nbsp;Alan Roger Santos-Silva,&nbsp;Ciro Dantas Soares,&nbsp;Jacks Jorge","doi":"10.1111/jop.70022","DOIUrl":"10.1111/jop.70022","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Salivary gland oncocytomas are infrequent benign salivary gland tumors with few reported cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>This study aimed to systematically review case reports and case series studies on oncocytomas in the head and neck region.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials &amp; Methods</h3>\u0000 \u0000 <p>Electronic searches were performed in PubMed, Scopus, Web of Science, Embase, and LILACS databases. The risk of bias was assessed using the Joanna Briggs Institute—University of Adelaide tool for case reports and case series.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 99 studies (145 cases) were included. Oncocytomas predominantly affected women, typically presenting as solitary, asymptomatic parotid masses in patients over 51 years of age. Some cases reported multiple or bilateral tumors, occasionally associated with other salivary gland lesions. Histologically, the tumors were primarily composed of eosinophilic oncocytes with minimal pleomorphism, arranged in diverse architectural patterns. Immunohistochemical analysis revealed positivity for PTAH, antimitochondrial antigen, CK5/6, CK8/18, CK10/13, CK19, EMA, along with a low Ki67 index, while being negative for S100 and actin. Surgical excision was the primary treatment, with rare instances of recurrence.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Oncocytoma is a rare, benign neoplasm that most commonly arises in the parotid gland, with a predilection for female patients. Complete surgical excision constitutes the standard treatment and is associated with an excellent prognosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"635-646"},"PeriodicalIF":2.3,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}