{"title":"侵袭性肿瘤前部肿瘤细胞中高表达的NELFE是口腔鳞状细胞癌的预后生物标志物。","authors":"Yizhuo Xue, Lingyun Liu, Liyuan Yu, Zihui Li, Xiaofeng Huang, Sheng Chen, Yue Jing, Xiaoxin Zhang, Liang Ding, Yuxian Song, Zhiyong Wang, Yanhong Ni","doi":"10.1111/jop.70025","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Negative elongation factor E (NELFE) is a crucial subunit of the NELF complex, which plays pivotal roles in transcriptional regulation and cell fate. Despite the role of dysregulated NELFE in promoting the onset and progression of some kinds of tumors, its expression profile, prognostic value, and function in oral squamous cell carcinoma (OSCC) still remain unknown.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Immunohistochemistry (IHC) was utilized to analyze the NELFE expression profile in OSCC, and the prognostic values of NELFE were also evaluated. qRT-PCR and Western blot were used to compare the expression of NELFE in OSCC cell lines. NELFE expression was knocked down by small interfering RNA fragments, and functional experiments were conducted to investigate the impact of NELFE on the proliferation and migration of OSCC cell lines. Gene set enrichment analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data to explore potential mechanisms of NELFE in OSCC.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>NELFE was widely distributed throughout tumor cells (TCs), fibroblast-like cells (FLCs), and tumor infiltrating lymphocytes (TILs) in OSCC tissue. However, its expression was significantly upregulated in TCs at the invasive tumor front compared to the tumor center components (<i>p</i> = 0.027). Higher expression of NELFE in TCs at the invasive tumor front (ITF) was significantly associated with higher lymph node metastasis (<i>p</i> = 0.024), worse invasion pattern (<i>p</i> < 0.0001), and more advanced tumor node metastasis classification (<i>p</i> = 0.002). Additionally, higher expression of NELFE in TCs at the invasive tumor front was also linked to higher recurrence (<i>p</i> = 0.021) and shorter disease-free (HR = 2.045, <i>p</i> = 0.006) as well as relapse-free survival (HR = 2.369, <i>p</i> = 0.011). The NELFE mRNA and protein were generally expressed in OSCC cells. Functionally, silencing NELFE significantly inhibited the proliferation and migration capabilities of OSCC tumor cells in vitro. Finally, GSEA revealed a significant difference in the E2F pathway between NELFE high and low expression.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>NELFE expression at the invasive front was associated with poor prognosis and could be a potential prognostic biomarker for OSCC.</p>\n </section>\n </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 8","pages":"757-769"},"PeriodicalIF":2.3000,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly Expressed NELFE in Tumor Cells at the Invasive Tumor Front Was a Prognostic Biomarker for Oral Squamous Cell Carcinoma\",\"authors\":\"Yizhuo Xue, Lingyun Liu, Liyuan Yu, Zihui Li, Xiaofeng Huang, Sheng Chen, Yue Jing, Xiaoxin Zhang, Liang Ding, Yuxian Song, Zhiyong Wang, Yanhong Ni\",\"doi\":\"10.1111/jop.70025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Negative elongation factor E (NELFE) is a crucial subunit of the NELF complex, which plays pivotal roles in transcriptional regulation and cell fate. Despite the role of dysregulated NELFE in promoting the onset and progression of some kinds of tumors, its expression profile, prognostic value, and function in oral squamous cell carcinoma (OSCC) still remain unknown.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Immunohistochemistry (IHC) was utilized to analyze the NELFE expression profile in OSCC, and the prognostic values of NELFE were also evaluated. qRT-PCR and Western blot were used to compare the expression of NELFE in OSCC cell lines. NELFE expression was knocked down by small interfering RNA fragments, and functional experiments were conducted to investigate the impact of NELFE on the proliferation and migration of OSCC cell lines. Gene set enrichment analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data to explore potential mechanisms of NELFE in OSCC.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>NELFE was widely distributed throughout tumor cells (TCs), fibroblast-like cells (FLCs), and tumor infiltrating lymphocytes (TILs) in OSCC tissue. However, its expression was significantly upregulated in TCs at the invasive tumor front compared to the tumor center components (<i>p</i> = 0.027). Higher expression of NELFE in TCs at the invasive tumor front (ITF) was significantly associated with higher lymph node metastasis (<i>p</i> = 0.024), worse invasion pattern (<i>p</i> < 0.0001), and more advanced tumor node metastasis classification (<i>p</i> = 0.002). Additionally, higher expression of NELFE in TCs at the invasive tumor front was also linked to higher recurrence (<i>p</i> = 0.021) and shorter disease-free (HR = 2.045, <i>p</i> = 0.006) as well as relapse-free survival (HR = 2.369, <i>p</i> = 0.011). The NELFE mRNA and protein were generally expressed in OSCC cells. Functionally, silencing NELFE significantly inhibited the proliferation and migration capabilities of OSCC tumor cells in vitro. Finally, GSEA revealed a significant difference in the E2F pathway between NELFE high and low expression.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>NELFE expression at the invasive front was associated with poor prognosis and could be a potential prognostic biomarker for OSCC.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16588,\"journal\":{\"name\":\"Journal of Oral Pathology & Medicine\",\"volume\":\"54 8\",\"pages\":\"757-769\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Pathology & Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jop.70025\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Pathology & Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jop.70025","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Highly Expressed NELFE in Tumor Cells at the Invasive Tumor Front Was a Prognostic Biomarker for Oral Squamous Cell Carcinoma
Background
Negative elongation factor E (NELFE) is a crucial subunit of the NELF complex, which plays pivotal roles in transcriptional regulation and cell fate. Despite the role of dysregulated NELFE in promoting the onset and progression of some kinds of tumors, its expression profile, prognostic value, and function in oral squamous cell carcinoma (OSCC) still remain unknown.
Methods
Immunohistochemistry (IHC) was utilized to analyze the NELFE expression profile in OSCC, and the prognostic values of NELFE were also evaluated. qRT-PCR and Western blot were used to compare the expression of NELFE in OSCC cell lines. NELFE expression was knocked down by small interfering RNA fragments, and functional experiments were conducted to investigate the impact of NELFE on the proliferation and migration of OSCC cell lines. Gene set enrichment analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data to explore potential mechanisms of NELFE in OSCC.
Results
NELFE was widely distributed throughout tumor cells (TCs), fibroblast-like cells (FLCs), and tumor infiltrating lymphocytes (TILs) in OSCC tissue. However, its expression was significantly upregulated in TCs at the invasive tumor front compared to the tumor center components (p = 0.027). Higher expression of NELFE in TCs at the invasive tumor front (ITF) was significantly associated with higher lymph node metastasis (p = 0.024), worse invasion pattern (p < 0.0001), and more advanced tumor node metastasis classification (p = 0.002). Additionally, higher expression of NELFE in TCs at the invasive tumor front was also linked to higher recurrence (p = 0.021) and shorter disease-free (HR = 2.045, p = 0.006) as well as relapse-free survival (HR = 2.369, p = 0.011). The NELFE mRNA and protein were generally expressed in OSCC cells. Functionally, silencing NELFE significantly inhibited the proliferation and migration capabilities of OSCC tumor cells in vitro. Finally, GSEA revealed a significant difference in the E2F pathway between NELFE high and low expression.
Conclusions
NELFE expression at the invasive front was associated with poor prognosis and could be a potential prognostic biomarker for OSCC.
期刊介绍：
The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
