Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-01-29 DOI:10.1111/jop.13606

Katarina Zeljic, Dunja Pavlovic, Goran Stojkovic, Sandra Dragicevic, Jelena Ljubicic, Nikola Todorovic, Aleksandra Nikolic

{"title":"Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers","authors":"Katarina Zeljic, Dunja Pavlovic, Goran Stojkovic, Sandra Dragicevic, Jelena Ljubicic, Nikola Todorovic, Aleksandra Nikolic","doi":"10.1111/jop.13606","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>A recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes <i>TNS3</i> and <i>LRRFIP1</i> in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>Our aim was to investigate the biomarker potential of TNS3-203 and LRRFIP1-211 transcripts in oral cancer, the most common type of head and neck cancer.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>An in silico approach was used to characterize the promoters and transcripts of interest. Relative expression of TNS3-203 and LRRFIP1-211 transcripts was evaluated by qRT-PCR in a group of 46 oral cancer patients in samples of cancer and adjacent non-cancerous tissue.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>TNS3-203 was significantly overexpressed in oral cancer compared with matched non-cancerous tissue, so this transcript can potentially be used as a diagnostic biomarker. There were no differences in LRRFIP1-211 level between analyzed tissues. None of the investigated transcripts has prognostic potential in oral cancer.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The results obtained indicate the diagnostic potential of TNS3-203, but not LRRFIP1-211 transcript and its role in oral carcinogenesis.</p>\n </section>\n </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"151-160"},"PeriodicalIF":2.3000,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Pathology & Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jop.13606","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

A recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes TNS3 and LRRFIP1 in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211.

Objective

Our aim was to investigate the biomarker potential of TNS3-203 and LRRFIP1-211 transcripts in oral cancer, the most common type of head and neck cancer.

Materials and Methods

An in silico approach was used to characterize the promoters and transcripts of interest. Relative expression of TNS3-203 and LRRFIP1-211 transcripts was evaluated by qRT-PCR in a group of 46 oral cancer patients in samples of cancer and adjacent non-cancerous tissue.

Results

TNS3-203 was significantly overexpressed in oral cancer compared with matched non-cancerous tissue, so this transcript can potentially be used as a diagnostic biomarker. There were no differences in LRRFIP1-211 level between analyzed tissues. None of the investigated transcripts has prognostic potential in oral cancer.

Conclusion

The results obtained indicate the diagnostic potential of TNS3-203, but not LRRFIP1-211 transcript and its role in oral carcinogenesis.

查看原文本刊更多论文

TNS3-203和LRRFIP1-211转录物作为口腔癌生物标志物的分析

最近的一项泛癌转录组分析表明，与非癌组织相比，头颈部鳞状细胞癌中TNS3和LRRFIP1基因的替代启动子的活性存在差异。在头颈部鳞状细胞癌中上调的启动子调控转录本TNS3-203和LRRFIP1-211的表达。目的：我们的目的是研究TNS3-203和LRRFIP1-211转录本在口腔癌（最常见的头颈癌）中的生物标志物潜力。材料和方法：采用计算机方法对感兴趣的启动子和转录本进行表征。采用qRT-PCR技术对46例口腔癌患者癌组织及癌旁非癌组织样本中TNS3-203和LRRFIP1-211转录本的相对表达量进行评估。结果：与匹配的非癌组织相比，TNS3-203在口腔癌中显著过表达，因此该转录物可能被用作诊断性生物标志物。LRRFIP1-211水平在分析组织间无差异。所研究的转录本均不具有口腔癌的预后潜力。结论：本研究结果提示TNS3-203具有诊断潜力，而LRRFIP1-211转录本在口腔癌变中的作用尚不明确。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.