Journal of medicinal food最新文献

{"title":"Comparing the Bioavailability of Two Seawater-Derived Magnesium Preparations.","authors":"Brian K McFarlin, Anyla L Paschall, Molly E Kelly, Stephanie M Womack","doi":"10.1177/1096620X251380191","DOIUrl":"10.1177/1096620X251380191","url":null,"abstract":"<p><p>Magnesium deficiency is a common problem worldwide; however, existing magnesium dietary supplement sources require large doses to overcome low bioavailability. Previously research has established that seawater contains magnesium in addition to 72 other trace and ultratrace minerals, resulting in better bioavailability than traditional magnesium sources. The purpose of the present study was to compare the bioavailability of magnesium obtained from two different seawater processing methods (hydroxide vs. citrate). In a double-blind manner, healthy, young men and women (<i>N</i> = 20) completed three trials using a crossover design: placebo (maltodextrin), seawater magnesium citrate (Aquamin®-Mg; min 10% elemental magnesium), and seawater-derived magnesium hydroxide (Aquamin®-MgTg; min 33% elemental magnesium). Total magnesium doses were standardized on the Recommended Daily Allowance (RDA) for elemental magnesium. An incremental, 18-h urine magnesium excretion test was used to assess relative magnesium bioavailability. The urine uptake was verified by short-term serum magnesium measurements (1- and 2-h postingestion). Serum and urine magnesium concentration were analyzed in triplicate using a colorimetric assay. We found that both seawater-derived magnesium preparations significantly increased magnesium absorption compared with placebo (>97% change) but did not differ from each other when standardized on magnesium dose. The magnesium hydroxide form may be particularly useful since its greater magnesium content allows for ingestion of smaller total quantities compared to soluble magnesium citrate.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular Evaluations of a Mixed Extract of <i>Salvia Miltiorrhiza Bunge</i> and <i>Paeonia Lactiflora</i> Pall. (USCP-GVH-014): A 12 Weeks Clinical Perspective.","authors":"Hyungyung Chai, Juah Son, Sukjin Song, Mi-Ran Cha, Byulnim Oh, Seyl Kim, Hyeon Gyu Lee, Soon Ae Kim","doi":"10.1177/1096620X251377431","DOIUrl":"https://doi.org/10.1177/1096620X251377431","url":null,"abstract":"<p><p><i>Salvia miltiorrhiza Bunge</i> and <i>Paeonia lactiflora</i> Pall. are traditionally used to manage cardiovascular health. However, clinical evidence evaluating standardized extracts for specific cardiovascular benefits is still evolving. This study aimed to evaluate its efficacy and safety of a mixed extract of <i>S. miltiorrhiza Bunge</i> and <i>P. lactiflora</i> Pall. (USCP-GVH-014) for improving cardiovascular function in adults with early-stage vascular health decline. This 12-week, single-center, single-arm, prospective exploratory clinical trial enrolled 30 adults with at least two risk factors. Participants consumed USCP-GVH-014 (1200 mg/day), and outcomes included systolic blood pressure (SBP), carotid intima-media thickness (CIMT), lipid metabolism markers, and inflammatory markers, which were assessed at baseline, 6 weeks, and 12 weeks. USCP-GVH-014 significantly reduced SBP over time (<i>P</i> = .013), particularly at 12 weeks (<i>P</i> = .007). Total cholesterol significantly decreased at 6 weeks (<i>P</i> = .035), though the effect was not sustained at 12 weeks. Low-density lipoprotein cholesterol demonstrated a significant overall reduction (<i>P</i> = .031), but post-hoc comparisons did not confirm the significance between specific time points. CIMT significantly decreased after 12 weeks (<i>P</i> < .001). Additionally, improvements were observed in mean arterial pressure (<i>P</i> = .008), pulse pressure (<i>P</i> = .04), heart rate (<i>P</i> = .013), and right pulse wave velocity (<i>P</i> = .043). No serious adverse events related to the product were reported. USCP-GVH-014 may enhance vascular health by lowering SBP, reducing CIMT, and modulating lipid metabolism, highlighting its potential as a functional ingredient for cardiovascular health support.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmon Nasal Cartilage-Derived Proteoglycans Alleviate Cartilage Degeneration in Osteoarthritis by Modulating Inflammation and Apoptosis.","authors":"Jinhee Kim, Jeongjin Park, Seong-Hoo Park, Yuri Gwon, Jinhak Kim, Hideharu Nakano, Tomohiro Okazaki, Minhee Lee","doi":"10.1177/1096620X251372437","DOIUrl":"https://doi.org/10.1177/1096620X251372437","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a chronic degenerative joint disease characterized by progressive cartilage damage, inflammatory responses, and apoptosis of chondrocytes. In this study, we investigated the therapeutic potential properties of proteoglycans (PG) extracted from salmon nasal cartilage in both <i>in vitro</i> (HTB-94 human chondrocytic cells) and <i>in vivo</i> (monosodium iodoacetate-induced OA rat model) approaches. Rats were treated with PG, and key parameters related to cartilage integrity, inflammation, and apoptosis were evaluated. Our results showed that PG treatment significantly improved cartilage structure and decreased inflammation, as evidenced by decreased levels of PGE₂ and nitric oxide, as well as reduced expression of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1<i>β</i>, and interleukin-6. PG also downregulated matrix metalloproteinases while increasing tissue inhibitors of metalloproteinases, preserving cartilage integrity. Additionally, apoptotic signaling pathways including JNK/c-Fos/c-Jun and FADD/capase-8/caspase-3 were attenuated, and the Bax/Bcl-2 ratio was favorably modulated by PG. These findings suggest that PG can protect articular cartilage by mitigating inflammation, preserving cartilage degradation, and preventing chondrocyte apoptosis. This study supports the potential therapeutic role of PG as a promising treatment option for OA, providing both anti-inflammatory and chondroprotective effects.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus subtilis</i> pb2441 Ameliorates Hepatic Steatosis by Decoupling Liver and Fat Tissue Lipid Accumulation in a High-Fat Diet-Fed Mouse Model.","authors":"Jung-Yun Lee, Sung-Su Park, Il Kyu Cho, Kyoung-Sik Moon, Yangrae Cho","doi":"10.1177/1096620X251372435","DOIUrl":"10.1177/1096620X251372435","url":null,"abstract":"<p><p>Nonalcoholic fatty liver disease (NAFLD) is a global health issue, often associated with gut dysbiosis. In recent years, probiotics have gained attention as potential therapeutic agents for NAFLD. This study explored the effects of a single strain, <i>Bacillus subtilis</i> with high surfactin secretion, on C57BL/6 mice fed a high-fat diet (HFD), a model for NAFLD, for 13 weeks. We conducted efficacy assays over 13 weeks on liver fat accumulation and gut microbiome modulation. <i>Bacillus</i> supplementation reduced body weight gain and fat accumulation in the liver, but not in adipose tissues. This indicates a decoupling of hepatic and adipose lipid accumulation-meaning that lipid reduction occurred selectively in the liver, independent of changes in peripheral fat storage. Hepatic steatosis and liver enzyme levels were significantly improved. The supplementation largely maintained or amplified the bacterial abundance shifts caused by the HFD. Only seven-including <i>Lactobacillus</i>, <i>Akkermansia</i>, and <i>Romboutsia</i>-out of 53 bacterial genera which were significantly changed by HFD were restored to normal levels by the supplementation. These three genera are commonly regarded as beneficial for human health due to their roles in gut barrier integrity, immune modulation, and metabolic regulation. In contrast, despite these limited changes in bacterial composition, bacterial enzyme analysis suggested significant metabolic modulation by <i>Bacillus</i> supplementation. A single strain of <i>Bacillus subtilis</i>, instead of a mixture of multiple bacterial strains, can prevent hepatic steatosis without affecting fat tissue weight, underscoring its potential as a targeted therapeutic option through microbiome modulation of a few beneficial bacteria. [Figure: see text].</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Craibiodendron stellatum</i> (Pierre) WW Sm. Extract Suppresses Lipopolysaccharide-Induced Acute Lung Injury by Regulating Macrophage-Mediated Inflammation <i>In Vivo</i> and <i>In Vitro</i>.","authors":"Da Hye Gu, Min Jeong Kim, Ardina Nur Fauziah, Sung Keun Jung","doi":"10.1177/1096620X251360148","DOIUrl":"10.1177/1096620X251360148","url":null,"abstract":"<p><p>Acute lung injury (ALI), primarily triggered by bacterial infection, occurs via intricate immune defense mechanisms within the lungs. This study aimed to investigate the potential of <i>Craibiodendron stellatum</i> (Pierre) WW Sm. extract (CSE) as an anti-inflammatory agent in a lipopolysaccharide (LPS)-induced C57BL/6 mouse ALI model and RAW 264.7 macrophages. Results showed that oral CSE administration significantly suppressed LPS-induced ALI symptoms, including hemorrhage in alveolar spaces, thickened alveolar walls, and inflammatory cell infiltration; it also inhibited the production of proinflammatory cytokines in the lungs. Additionally, oral CSE administration attenuated the LPS-induced iNOS expression in mouse lungs. CSE also inhibited the LPS-induced nuclear factor-kappa B signaling in RAW 264.7 cells. DCF-DA assay confirmed that CSE could reduce the LPS-induced reactive oxygen species production. Our findings suggested that CSE may be useful as a functional respiratory health material by regulating the inflammatory signaling pathway in alveolar macrophages in ALI induced by bacterial infections.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"934-946"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor Effect of Isoliquiritigenin via Mitochondrial Dysfunction and Oxidative Stress in Cholangiocarcinoma Cells.","authors":"Sang Hyeok Bae, Hwayoung Na, Dohee Ahn, Hong Kyu Lee, Kyung-Chul Choi","doi":"10.1089/jmf.2024.k.0280","DOIUrl":"10.1089/jmf.2024.k.0280","url":null,"abstract":"<p><p>Isoliquiritigenin (ISL), a bioactive phytochemical derived from the root of <i>Glycyrrhiza uralensis</i>, is known to exert anticancer effects by modulating cancer cell functions, including proliferation, metastasis, and autophagy. However, the anticancer effects of ISL on cholangiocarcinoma (CCA) are limited. In this study, we investigated the anticancer effects of ISL on CCA cells (SNU-478 and HuCCT-1). In SNU-478 cells, ISL decreased the expression of glucose-regulated protein 78 (GRP78). ISL was also found to significantly reduce cell viability and colony area of both SNU-478 and HuCCT-1 cells. Furthermore, ISL treatment significantly increased the population of apoptotic cells, as observed through Annexin V/propidium iodide (PI) staining. PI staining showed that the ISL treatment induced significant cell cycle arrest compared to the control group, indicating that ISL-induced decreases in cell viability are associated with cell cycle arrest and apoptosis. ISL treatment also resulted in a significant decline in the mitochondrial membrane potential when observed through JC-10 staining. Mitochondrial superoxide staining revealed that ISL significantly upregulated the generation of mitochondrial reactive oxygen species (ROS). Acridine orange staining indicated that the ISL treatment upregulated lysosomal membrane permeability, which could be related to autophagy. These results suggest that ISL affects the proliferation and apoptosis of CCA cells, which might be related to its regulatory role in various cellular functions, including mitochondrial dysfunction, ROS balance, and autophagy. This study provides empirical evidence that ISL could be a potential candidate for CCA therapy.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"909-920"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Microencapsulated and Nonencapsulated Aronia Extract on Paraoxonase 1 Gene Expression and Aortic Histopathology in High-Fat Diet-Fed Rats.","authors":"Serim Tuna Koç, Süleyman Kök, Sertaç Atalay, Onur Ersoy","doi":"10.1089/jmf.2025.0027","DOIUrl":"10.1089/jmf.2025.0027","url":null,"abstract":"<p><p>The aim of this study was to determine the effects of microencapsulated and nonencapsulated aronia (<i>Aronia melanocarpa</i>) extract on paraoxonase 1 (PON1) mRNA expression, HDL cholesterol, and aortic atherosclerosis in rat blood and liver tissues. The study involved 42 male Sprague-Dawley rats aged 10 weeks. The experimental groups were as follows: (1) standard diet control (CON), (2) high-fat diet (HF) control, (3) HF + 400 mg/kg aronia extract (HF400E), (4) HF + 200 mg/kg aronia extract (HF200E), (5) HF + 400 mg/kg microencapsulated aronia (HF400C), and (6) HF + 200 mg/kg microencapsulated aronia (HF200C). The rats were fed a HF between the 10th and 20th week, and at the end of the 20th week, all groups except CON were administered aronia extract (200-400 mg/kg) by oral gavage for 6 weeks. After 6 weeks, biochemical analyses and aortic histology evaluations were performed. The results showed that the HF significantly increased serum total cholesterol, triglycerides, low-density lipoprotein levels, and hepatic malondialdehyde levels. However, administration of HF400E significantly reduced these parameters in hyperlipidemic rats. Serum PON1 enzyme activity was significantly higher in the HF400E group compared with both the CON (<i>P</i> < .001) and HF (<i>P</i> < .0025) groups. Although PON1 mRNA levels in the HF400E, HF200E, and HF200C groups were higher than in the CON and HF groups, the differences were not statistically significant. Evaluation of the aortic tissues showed normal tissue morphology in all experimental groups, with no signs of atherosclerotic plaques, hyaline or mucoid changes, or endothelial anomalies in the thoracic or aortic arch tissues. These findings suggest that aronia, rich in anthocyanins and other polyphenolic compounds, may help prevent and slow down atherosclerosis by enhancing PON1 enzyme activity, which is involved in preventing lipid oxidation, the first step in atherosclerosis.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"885-896"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ursolic Acid Attenuates Inflammatory and Severity of Osteonecrosis in Rats Treated with Zoledronic Acid.","authors":"Lívia Moreira Caetano Coelho, Larissa Mourão Carvalho, José Vitor Mota Lemos, Cláudia do Ó Pessoa, Maria Júlia Barbosa Bezerra, Lia Vila Real Lima, Maria Elisa Quezado Lima Verde, Paulo Goberlânio de Barros Silva, Fabrício Bitu Sousa, Thinali Sousa Dantas","doi":"10.1089/jmf.2023.0167","DOIUrl":"10.1089/jmf.2023.0167","url":null,"abstract":"<p><p>Bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ) alters osteoclast function. Ursolic acid (UA) can inhibit the expression of interleukin-17 (IL-17). Evaluate the influence of UA treatment on the severity of BRONJ in zoledronic acid (ZA)-treated rats' jaws. Fifty male Wistar rats were used, divided into a negative control group (0.1 mL/kg sterile saline), a positive control group (ZA, 0.20 mg/kg), and three test groups treated with ZA and UA 10, 20 or 40 mg/kg by gavage every three days from the beginning of the protocol until euthanasia. After three consecutive weekly administrations of ZA intravenous (i.v.), exodontia of the 1st left lower molar was performed, administration of an additional dose of ZA, and euthanasia after 28 days from exodontia. Hemimandibles were removed for radiographical, histological, and immunohistochemical analysis and gum samples for Western blotting. The femur was removed for the three-point bending test. Analysis of variance (ANOVA)/Bonferroni was used. Radiographically, UA reduced the area suggestive of OM (7.2  ±  0.6 vs. 5.2 ±  0.4, <i>P</i> = .015) and the highest dose of UA reversed the number of nonviable osteocytes (80.3  ±  4.9 vs. 55.4 ±  4.6, <i>P</i> = .007), suggesting bone healing through IL-17 inhibition. UA reduced the number of polymorphonuclear cells (208 ± 17 vs. 30 ± 9, <i>P</i> < .001), mononuclear cells (207 ± 34 vs. 74 ± 20, <i>P</i> < 0.001) and apoptotic osteoclasts (87 ± 4 vs. 61 ± 3, <i>P</i> < .001), observing that these parameters are higher in groups treated only with ZA. The two highest doses of UA reduced the immunoexpression of IL-17 (429 ± 45 vs. 300 ± 42, <i>P</i> = .014) and increased the percentage of circulating lymphocytes (69 ± 2 vs. 82 ± 2, <i>P</i> < .001). AZ increased the expression of RORyT and the highest dose of UA (1.887  ±  0.114 vs. 0.869  ±  0.050, <i>P</i> < .001),) reduced this expression, suggesting that UA may be a specific antagonist of RORyT, which has the capacity to inhibit the expression of this protein. UA promise in reducing the severity of ZA-induced ONJ.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"860-868"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Lactobacillus</i> spp. Supplementation for Improving Muscle Health: A Systematic Review and Meta-Analysis.","authors":"Jeong Yeon Im, Eun Jin Jung, Jun Gu Lee, Bok Kyung Han, Ji Youn Hong, Young Jun Kim","doi":"10.1177/1096620X251362009","DOIUrl":"10.1177/1096620X251362009","url":null,"abstract":"<p><p>This systematic review and meta-analysis aimed to assess the quantitative effects of probiotic supplementation on improving muscle health, including muscle mass, lean mass, and hand grip strength, compared with a placebo. Databases were searched from PubMed, Embase, and Cochrane Library through January 2024. Researchers independently reviewed the studies using the quality assessment tool. Sensitivity analysis was conducted to clarify the statistical heterogeneity of the included studies. Funnel plots and Egger's test were used to assess the potential for publication bias in the meta-analysis. The overall estimates showed that muscle mass (standardized mean difference [SMD] = 0.29; 95% confidence interval [CI] = 0.03, 0.55; <i>P</i> = .03) and hand grip strength [SMD = 0.57; 95% CI = 0.09, 1.04; <i>P</i> = .02] were significantly increased. However, lean mass was not significantly changed (SMD = -0.05; 95% CI = -0.20, 0.10; <i>P</i> = .51). The subgroup studies demonstrated a significant size effect on muscle mass over 10 weeks of probiotic supplementation (SMD = 0.50; 95% CI = 0.02, 0.98; <i>I</i>² = 78%; <i>P</i> < .01). Moreover, the ethnicity subgroup comparison between Asian and non-Asian participants evaluating the effects of probiotic supplementation on muscle mass showed that Asian participants (SMD = 0.36; 95% CI = 0.03, 0.69) exhibited statistically significant heterogeneity (<i>I</i>² = 65%; <i>P</i> < .01) compared with non-Asian participants. Nevertheless, further studies are needed to elucidate the mechanism and evaluate the scientific evidence and clinical verification of probiotic supplementation. In conclusion, long-term probiotic supplementation with <i>Lactobacillus</i> spp. improved muscle function, increasing muscle mass and hand grip strength, especially among Asian participants, showing greater muscle strength gains from probiotic supplementation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"842-859"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Abelmoschus manihot</i> Flower Extract Retards Platelet-Derived Growth Factor-BB-Stimulated Proliferation and Migration in Vascular Smooth Muscle Cells by Inhibiting the MAPK/NF-κB Pathway and Matrix Metalloproteinase Expressions.","authors":"Chin-Feng Hsuan, Yi-Ting Kuo, Tzu-Hsien Chang, Ya-Ling Chen, Hsin-Ya Houng, Natasha Chang, Sabrina Chang, Chi-Chang Chang, Jer-Yiing Houng","doi":"10.1089/jmf.2024.k.0263","DOIUrl":"10.1089/jmf.2024.k.0263","url":null,"abstract":"<p><p>Vascular smooth muscle cells (VSMCs) are vital to the structure of blood vessel walls. Under abnormal vascular conditions, VSMCs undergo a phenotypic transformation, leading to enhanced cell proliferation, migration, and matrix synthesis. This contributes to the development of vascular diseases such as atherosclerosis, arteriosclerosis, and restenosis. During this process, platelet-derived growth factor (PDGF)-BB is a key inducer of the VSMC phenotypic transformation. <i>Abelmoschus manihot</i> (L.) Medic flower (AMf) is known for its rich nutritional value and traditional medicinal uses. Its extract has been clinically used to treat kidney diseases, but its impact on VSMCs has not been documented. This study explored the inhibitory effects of AMf ethanol extract (AME), hot water extract (AMW), and supercritical CO₂ extract (AMS), and their five indicator components (rutin, quercetin, isoquercitrin, myricetin, and hyperoside) on PDGF-BB-stimulated proliferation and migration using a rat aortic smooth muscle cell (RASMC) model. Both AME and AMS showed a significant dose-dependent inhibition of PDGF-BB-induced RASMC proliferation and migration, with AME being more effective than AMS. In contrast, AMW had no effect. The five indicator compounds also showed excellent inhibitory effects. AME treatment effectively reduced the phosphorylation of JNK, ERK, p38, and NF-κB, and downregulated the expressions of the migration-promoting factors <i>MMP-2</i> and <i>MMP-9</i> in PDGF-BB-stimulated RASMCs. These findings suggest that AME protects VSMCs by regulating the phosphorylation of the MAPK/NF-κB pathway and suppressing MMP expression. Consequently, AME may help prevent or slow the progression of vascular diseases.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"921-933"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}