Journal of medicinal food最新文献

{"title":"Ketogenic Diet for Epilepsy: The Olive Oil Effect to Optimization. A Narrative Review.","authors":"Sofia Zouganeli, Mary Yannakoulia, Achilleas Attilakos, Smaragdi Fessatou, Evdokia K Mitsou, Adamantini Kyriacou, Argirios Dinopoulos","doi":"10.1089/jmf.2025.0009","DOIUrl":"https://doi.org/10.1089/jmf.2025.0009","url":null,"abstract":"<p><p>The ketogenic diet (KD) has long been used in the treatment of drug-resistant epilepsy, demonstrating significant beneficial health effects. Various modifications of the KD have been introduced as alternatives to the classical, more restrictive form, aiming to improve patient's adherence while maintaining therapeutic efficacy. Among these adaptations, the Mediterranean ketogenic diet (MedKD) has been primarily implemented in populations in the Mediterranean region. The MedKD integrates elements of the traditional Mediterranean diet, with olive oil as the primary fat source, leveraging its unique properties. This narrative review examines the possible connections between olive oil and the mechanisms of KD, proposing the extra virgin olive oil-rich MedKD as a healthier option with the potential for reduced adverse effects.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Curative Effects of Vitamins D3&K2 on the Expressions of p53, Bax, Bcl-2, and Oxidative Stress Induced by Methotrexate in ZFL Epithelial Cells.","authors":"Güllü Kaymak","doi":"10.1089/jmf.2024.0261","DOIUrl":"10.1089/jmf.2024.0261","url":null,"abstract":"<p><p>Methotrexate (MTX), a folate antimetabolite, is a cytotoxic drug known to cause cytotoxicity associated with free oxygen radicals. This study investigated the effect of vitamins D3 and K2 on MTX-induced liver cell injury using the zebrafish liver cell line (CRL-2643). Observed effects, levels of antioxidant enzymes, lipid peroxidation marker, and total antioxidant/oxidant status were evaluated by spectrophotometric methods. The mRNA expressions of p53, Bax, and Bcl-2 were measured using RT-PCR. In addition, acridine orange/ethidium bromide staining was performed to analyze the apoptosis status of the cells. The IC₅₀ value of MTX at 48 h was calculated as 442 <i>µ</i>g/mL with an MTT assay. The doses of D3&K2 are determined based on the recommended dose to be taken daily. As a result, this study suggests that MTX treatment induced oxidative damage on the liver cell, as assessed by increased reactive oxygen levels, lipid peroxidation, and decreased glutathione levels. In addition, while MTX increased the expression of the p53 and proapoptotic marker Bax, it decreased the expression of the anti-apoptotic factor Bcl-2. In conclusion, D3&K2 treatment protects against MTX-induced liver cell toxicity. It is thought that they can be used as a potential agent in clinical applications with MTX in treatment.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"657-667"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Correction to:</i> Indian Gooseberry and Barley Sprout Complex Prevent Oxidative Stress and Photoaging of the Skin in Ultraviolet B-Irradiated SHK-I Mice by Lee et al. Journal of Medicinal Food 2024;27(6):488-501; doi: 10.1089/jmf.2023.K.0229.","authors":"","doi":"10.1089/jmf.2023.K.0229.correx","DOIUrl":"10.1089/jmf.2023.K.0229.correx","url":null,"abstract":"","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"725-726"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Observation Battery Test for Single Intravenous Caffeine Exposure in Male ICR Mice.","authors":"Sung-Hyun Cho, Yeon-Woo Kim, Miae Doo, Jung-Heun Ha","doi":"10.1089/jmf.2024.k.0289","DOIUrl":"10.1089/jmf.2024.k.0289","url":null,"abstract":"<p><p>This study investigated the behavioral responses of male Institute for Cancer Research (ICR) mice to intravenous caffeine exposure via a functional observation battery (FOB) test. Thirty-two experimental mice were randomly assigned to four groups (<i>n</i> = 8 per group) and received intravenous caffeine at a dose of 0, 5, 10, or 20 mg/kg. Functional behaviors were observed at 0, 0.25, 1.5, 6, and 24 h after intravenous caffeine administration. Among the hand-held observations, the ease of removal from the cage and the ease of handling were significantly altered in all caffeine-exposed mice in both a dose-dependent and a time-dependent manner. In terms of physiological responses, both stimulus responses and locomotor activities were significantly affected by intravenous caffeine exposure. Specifically, the tail pinch response was significantly impaired in half of the mice in the 10 mg/kg and 20 mg/kg groups. Moreover, the rearing count decreased in the 10 mg/kg group at 1.5 to 6 h and in the 20 mg/kg group at 1.5 h after intravenous caffeine exposure. Furthermore, locomotor activity was markedly increased 0.25 h after intravenous caffeine administration in the 20 mg/kg group. These findings clearly indicate that intravenous caffeine exposure significantly impacts functional behaviors, as assessed by an FOB test, which is consistent with widely accepted safety pharmacology testing guidelines.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"719-724"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Schisandra Chinensis</i> Polysaccharide Ameliorates Renal Dysfunction by Inhibiting Inflammatory Reactions and Oxidative Stress in Diabetic Rats.","authors":"Chunmei Wang, Ting Li, Chengcheng Lin, Xudong Qiu, Wenyue Zhuang, He Li, Xingming Feng, Xingxu Du, Jinghui Sun","doi":"10.1089/jmf.2024.k.0285","DOIUrl":"10.1089/jmf.2024.k.0285","url":null,"abstract":"<p><p><i>Schisandra chinensis</i> (<i>S. chinensis</i>) polysaccharide (SCP) is an active ingredient from <i>S. chinensis</i> used mainly for the treatment of diabetes, owing to its antioxidant, hypoglycemic, and lipidemic-modulating activities. A rat type II diabetes mellitus model was established by giving rats a high-fat diet and streptozotocin (STZ) to investigate the protective effect of SCP against renal injury in diabetic rats. It was found in this study that fasting blood glucose, serum lipids, serum creatinine, and blood urea nitrogen levels were decreased, the insulin sensitivity was increased, and pathological injuries of the kidney were alleviated in SCP-treated groups, indicating that SCP should have a protective effect against renal injury in diabetic rats. SCP treatment reduced serum C-reactive protein and inhibited the expression of nuclear factors-κB and related inflammatory factors in the renal tissue of diabetic rats. SCP treatment also regulated the expression of Nuclear factor (erythroid-derived 2) like-2, heme oxygenase-1, and kelch-like ECH-associated protein-1, reduced serum malondialdehyde content, and increased superoxide dismutase activity. Furthermore, SCP down-regulated the expression of fibronectin, α-SMA, transforming growth factor β1, and p-Smad3, up-regulated Smad7 expression, and mitigated the collagen fiber deposition in the renal interstitium in diabetic rats. It can be concluded that the mechanism of SCP in alleviating renal injury may be related to inhibiting inflammation, increasing antioxidant stress capacity, and improving renal fibrosis in diabetic rats.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"682-693"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<b>Effects of Heat-Treated Green Tea Extract on Memory Function and Default Mode Network Connectivity in Individuals with Subjective Memory Impairment: A Randomized, Double-Blinded, Placebo-Controlled Trial</b>.","authors":"Yoonji Joo, Hyeonji Lee, Hyeonseok Jeong, Chaewon Suh, Haejin Hong, Yejin Kim, Seoyoung Yu, Chaerin Lee, Youngeun Shim, Sujung Yoon","doi":"10.1089/jmf.2024.k.0260","DOIUrl":"10.1089/jmf.2024.k.0260","url":null,"abstract":"<p><p>Given the increasing interest in the potential cognitive benefits of green tea, this study evaluated the efficacy of processed green tea products as nootropic agents. We conducted a 12-week, randomized, double-blinded, placebo-controlled trial to assess the effects of heat-treated green tea extract (HT-GTE) supplementation on memory performance in individuals with subjective memory impairment (SMI). A total of 80 participants with SMI (mean age 49.6 ± 5.6 years) were randomly assigned to either the HT-GTE group (<i>n</i> = 40) or the placebo group (<i>n</i> = 40). Memory performance was assessed using standardized composite scores at baseline and week 12. Additionally, we examined changes in functional connectivity (FC) within the default mode network (DMN), a key resting-state network associated with memory function, at both time points. Compared to the placebo group, the HT-GTE group demonstrated significant improvements in memory function after 12 weeks, as evidenced by a greater increase in composite scores (<i>z</i> = 2.535, <i>P</i> for interaction = .011). Furthermore, the HT-GTE group exhibited increased FC within the right precuneus region of the DMN (<i>z</i> = 2.554, <i>P</i> for interaction = .011). These findings underscore the potential of HT-GTE supplementation to enhance memory performance and FC within the DMN in middle-aged individuals with SMI, offering valuable insights into the cognitive benefits of green tea.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"708-718"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent Versus Continuous Wild Blueberry Feeding Alters Inflammation and Behavior in Aged Male Rats.","authors":"Barbara Shukitt-Hale, Derek R Fisher, Danielle S Cahoon, Marshall G Miller, Amanda N Carey, Tong Zheng","doi":"10.1089/jmf.2025.0001","DOIUrl":"10.1089/jmf.2025.0001","url":null,"abstract":"<p><p>Previous studies have shown that continuous daily consumption of polyphenolic-rich blueberries (BB) reduced neuroinflammation and improved age-related declines in motor and cognitive function in rodents and humans, but the optimal intake of BBs is currently unknown. To evaluate the beneficial effects of BBs on neuroinflammation and motor and cognitive function relative to the frequency of consumption, the diets of aged F344 rats (18 months; <i>n</i> = 15/group) were supplemented with either a continuous control diet, a continuous 2% wild BB diet, or an intermittent (3 days on, 4 days off) 2% wild BB diet for 2 months prior to behavioral testing. Because neuroinflammation has been identified as a major contributing factor to the behavioral declines seen in aging and neurodegenerative diseases, serum was collected pre-diet and at the end of the study to assess whether diet-induced changes in serum can alter the production of inflammatory stress signals in activated HAPI rat microglial cells <i>in vitro</i>. Serum from rats continuously and intermittently fed BBs had beneficial effects by reducing the expression of inflammatory enzymes cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in microglia, but only continuous feeding attenuated nitrite production. Intermittent BB-fed rats performed better on the rotarod and committed fewer errors in the radial arm water maze on Day 2 compared to control-fed animals. Based on these findings, BBs may not have to be consumed daily to elicit beneficial effects, possibly due to the retention of polyphenols and their metabolites in tissue and circulation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"639-646"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The <i>In Vitro</i> and <i>In Silico</i> Assessment of the Cytotoxic Effect of Daidzein Alone or in Combination with 5-Fluorouracil on Human Colorectal Adenocarcinoma Cell Line Caco-2.","authors":"Altinay Altinkaynak, Orhan Kocak, Esra Tanyel Akcit, Ece Simsek, Kubra Yildirim, Ahmet Yilmaz Coban","doi":"10.1089/jmf.2024.0132","DOIUrl":"10.1089/jmf.2024.0132","url":null,"abstract":"<p><p>We aimed to evaluate the cytotoxic effect of daidzein alone and in combination with 5-fluorouracil (5-FU) on Caco-2 cells and investigate potential interactions between daidzein and 5-FU with apoptosis-related tumor necrosis factor-related apoptosis-inducing ligand receptor-2, tumor necrosis factor -alpha receptor-1 and Interferon gamma receptor-1. In this study, we used trypan blue and MTT assays to evaluate the cytotoxic effect of daidzein and the effect of daidzein on the amount of substance P released from the cells using the ELISA method. We utilized the ligand-protein molecular docking method for our <i>in-silico</i> assessments. According to our cytotoxicity experiment results, daidzein decreased the proliferation of Caco-2 cells. It should be pointed out that our ELISA test results daidzein and 5-FU increase the level of Substance-P (SP), a proinflammatory cytokine released by Caco-2 cells. Based on our <i>in-silico</i> results, daidzein interacted with all three apoptosis-related receptors on the membrane of Caco-2 cells to a better extent than 5-FU. In sum, although daidzein increases the amount of SP, it is a compound that has significant cytotoxic effect on colon cancer cells and promise as a potential therapeutic agent for the treatment of colon cancer.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"647-656"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized Placebo Controlled Clinical Study of Dill (<i>Anethum Graveolens L.</i>) in Thyroiditis and Nodular Goiter Patients.","authors":"Sabahattin Destek, İlker Demirbolat, Ayşegül Yabacı, Murat Kalemoglu, Murat Kartal, Emine Yeliz Ersoy, Vahit Onur Gul, Zulal Kayıran, Mustafa Erhan Aysan","doi":"10.1089/jmf.2024.0263","DOIUrl":"10.1089/jmf.2024.0263","url":null,"abstract":"<p><p>Thyroid nodules are common and affect half of the general population by the age of 60 years. The cause is believed to be due to hypothyroidism, mutational changes, and autoimmunity leading to thyroid carcinoma. Dill, a traditional herbal remedy, has been used to treat thyroid dysfunctions such as hyperthyroidism and hypothyroidism. This study evaluates the effects of dill (<i>Anethum graveolens L.</i>) on patients with thyroiditis and nodular goiter. A study was conducted on patients with benign thyroid nodules. They were divided into two groups; placebo (=35) and dill group (<i>n</i> = 33). Dried and ground dill was put into hydroxypropyl methylcellulose capsules and patients were given three capsules per day for 90 days. Various tests were conducted at the beginning and end of the study, including thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), anti-thyroid peroxidase (anti-TPO), anti-thyroglobulin (anti-Tg), and C-reactive protein (CRP) analysis, and thyroid nodule dimensions were measured by ultrasound. Statistical analysis was performed. After 90 days of treatment with dill extract, patients showed significant reductions in TSH, FT4, anti-TPO, and CRP levels, as well as a decrease in thyroid nodule sizes as demonstrated by ultrasonography. The dill-treated group had significantly decreased TSH (<i>P</i> = .020), FT4 (<i>P</i> = .001), anti-TPO (<i>P</i> = .004), CRP (<i>P</i> = .003) levels, and nodule sizes (<i>P</i> = .001) at the beginning and end of the study. Compared to the control group, the dill group had significantly decreased TSH (<i>P</i> = .009), fT4 (<i>P</i> < .001), anti-TPO (<i>P</i> = .001), CRP (<i>P</i> < .001) levels and nodule sizes (<i>P</i> < .001), which reached normal values. <i>A. graveolens</i> suppressed inflammation of the thyroid gland, reduced nodule size, and normalized TSH levels. Dill supplementation should be recommended for the treatment of thyroid nodules in selected patients.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"668-672"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico and In Vivo Biological Evaluation of Extracts from Tunisian Desert Truffles (<i>Tirmania nivea</i> and <i>Terfezia boudieri</i>) on Scopolamine-Induced Memory Impairment in Mice.","authors":"Mohamed Ali Smach, Rihab Hammami, Amira Zarrouk, Jawhar Hafsa, Bassem Charfeddine, Khalifa Limem, Ayda Khadhri","doi":"10.1089/jmf.2023.0178","DOIUrl":"10.1089/jmf.2023.0178","url":null,"abstract":"<p><p>The present work focuses on studying the effectiveness of truffle extract administration to enhance memory functions in a scopolamine-induced amnesia mice model. Major phytochemical constituents, gallic acid and myricetin, underwent <i>in silico</i> studies using Autodock Vina and Discovery Studio visualizer software. Male mice were pretreated orally with either truffle extracts (400 mg/kg) or donepezil (3 mg/kg) for 21 days. Dementia was induced in mice by scopolamine (1 mg/kg) after 19 days of pretreatment and until day 28. The mice were divided into five experimental groups: (1) control; (2) model; (3) donepezil (1 mg/kg); (4) <i>Tirmania nivea</i> (400 mg/kg); and (5) <i>Terfezia boudieri</i> (400 mg/kg). Mice were subsequently subjected to a battery of behavioral tests, including an open field locomotor activity assay, and the Morris water maze (MWM) test. Biochemical, histopathological analyses and molecular docking were performed. Chronic Scopolamine administration decreased learning and memory enhancement during the MWM test. A significant increase in time to find the hidden platform and escape was observed. Scopolamine increases acetylcholinesterase (AChE) activity and oxidative stress. Conversely, treatment with the <i>T. nivea</i> and <i>T. boudieri</i> extracts (400 mg/kg orally administered) increased the memory of mice in the MWM task and suppressed scopolamine-induced oxidative damage. The memory enhancement effect was observed by the best docking scores of gallic acid and myricetin against AChE. This study reveals truffle's ameliorative effect on scopolamine-induced dementia in mice, possibly mediated by modulation of cholinergic activity and antioxidant activity. The docking of two major compounds, gallic acid and myricetin, predicted their potential as memory enhancers.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"673-681"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}