Journal of medicinal food最新文献

{"title":"A Mixture of Nicotinamide Mononucleotide, Decursin, and l-Cysteine Lowered Senescence-Associated Markers In Vitro and Was Effective Against 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis In Vivo: An Application of Network Pharmacology.","authors":"Hyo Ri Shin, La Yoon Choi, Seong Hwan Bae, Tae Gyun Kim, Hyung Taek Cho, Tae Jin Cho, Nam Su Oh, Youn Young Shim, Martin J T Reaney, Jeong Hoon Pan, Jeong-Oh Shin, Eui-Cheol Shin, Seong-Gyu Ko, Jinbong Park, Yohan Han, Kyung-Chul Choi, Young Jun Kim, Wan Heo, Kangwook Lee, Jae Kyeom Kim","doi":"10.1089/jmf.2024.k.0282","DOIUrl":"10.1089/jmf.2024.k.0282","url":null,"abstract":"<p><p>Skin aging, accelerated by oxidative stress from environmental factors, results in the breakdown of collagen and elastin, causing visible signs like wrinkles and inflammation. Natural antioxidants such as nicotinamide mononucleotide (NMN), decursin, and l-cysteine have shown potential in combating oxidative damage and inflammation. This study explored the effects of a formulated mixture of these compounds on skin aging and atopic dermatitis (AD) through a combination of <i>in vitro</i>, <i>in vivo</i>, and <i>in silico</i> methods. Using human keratinocyte cells, we assessed cytotoxicity via a cell viability assay, confirming NMN and l-cysteine were nontoxic up to 100 µM, while decursin exhibited toxicity above 10 µM. The intracellular oxidative stress measurement demonstrated that mixtures A and B, composed of the same concentrations of decursin and l-cysteine but differing in NMN levels (low in mixture A and moderate in mixture B), significantly reduced oxidative stress levels induced by 2,2'-azobis(2-amidinopropane) dihydrochloride, whereas mixture C, which contained the highest NMN concentration, was ineffective. Mixture B further reduced senescence-associated heterochromatin foci formation under oxidative stress, while mixture C caused cell structure disruptions. In the dinitrochlorobenzene-induced AD model in BALB/c mice, both, mixture-L and mixture-H treatments reduced epidermal thickness, scratching behavior, and transepidermal water loss, with mixture-L also lowering dermal thickness and mast cell infiltration. Gene expression analysis confirmed that mixture B decreased proinflammatory cytokines like <i>TNF-α</i> and <i>IL-6</i>, while network pharmacology predicted key antioxidant pathways, validated through restored <i>NOS2</i> gene expression. Overall, the findings highlight the potential of these compound mixtures to mitigate oxidative stress and inflammation, offering a promising approach for skin aging and dermatitis management, though further validation is needed to optimize efficacy and safety.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"490-500"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioimpedance Phase Angle as a Diagnostic Tool of Nutritional Status in Children with Crohn's Disease on Exclusive Enteral Nutrition Therapy: One-Year Follow-Up Study.","authors":"Agata Wasilewska, Przemysław Tomasik, Aleksander Gałaś, Anna Stochel-Gaudyn, Dorota Drożdż, Krzysztof Fyderek, Małgorzata Sładek","doi":"10.1089/jmf.2023.0242","DOIUrl":"10.1089/jmf.2023.0242","url":null,"abstract":"<p><p>Exclusive enteral nutrition (EEN) is highly effective in achieving remission and improving nutritional status in pediatric Crohn's disease (CD), commonly associated with altered body mass composition (BC). Bioimpedance analysis (BIA) with phase angle (PA) assessment is a noninvasive, reliable tool in screening for BC alterations. In the present study we aimed to assess the PA's usefulness in the detection of malnutrition in newly diagnosed pediatric CD, ongoing EEN treatment, and during 1-year follow-up. Patients and study design: Fourty-three patients with CD, qualified for EEN, were enrolled in the study. Additionally, 22 healthy children, being in the same age category, served as controls. Fat-free mass (FFM), fat-free mass index (FFMI), and BIA-derived PA were assessed at diagnosis, after EEN completion, and at week 52. The same parameters were obtained in the control group once. The majority of patients presented with normal body mass index at diagnosis. PA values were lower than 5 degrees in 65% of the study group. FFMI deficiencies were observed in 74% of patients. After EEN completion, an increase in FFM (<i>P</i> < .001) was observed. The highest mean of FFM (<i>P</i> < .001) and PA (<i>P</i> < .001) were observed at week 52. A strong correlation between PA and FFM at CD diagnosis (Spearman's rho = 0.671, <i>r</i> = 0.702, <i>P</i> < .001), after EEN completion (Spearman's rho = 0.781, <i>P</i> < .00) and at week 52 (Spearman's rho = 0.657, <i>P</i> < .001) was present. PA has been observed as positively associated with albumin concentrations both at diagnosis and after EEN. Our study provides some support that PA may be a reliable tool for screening alteration in BC, especially FFM and lean tissue mass (LTM). Moreover, PA has been observed as positively associated with albumin levels at CD diagnosis, which provides some evidence that it may be considered as a risk indicator of malnutrition and protein deficiency in newly diagnosed CD pediatric patients.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"501-507"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Centella Asiatica</i> Fraction-3 Enhances Antioxidant Capacity and Apoptotic Cell Death in HEK293 Kidney Cells.","authors":"Dhaneshree B Naidoo, Alisa Phulukdaree, Anil Armichund Chuturgoon, Vikash Sewram","doi":"10.1089/jmf.2022.0109","DOIUrl":"10.1089/jmf.2022.0109","url":null,"abstract":"<p><p>The traditional medicinal plant <i>Centella asiatica</i> is commonly used in Chinese and Ayurvedic medicine due to its vast range of therapeutic properties. Previously, the ethanolic <i>C. asiatica</i> leaf extract was subjected to silica column fractionation, and the C3 fraction was obtained. We investigated the antioxidant and anti-proliferative effects of C3 in human embryonic kidney (HEK293) cells. In HEK293 cells, C3 cytotoxicity was assessed (viability assay; 24 h; [0.2-3 mg/mL]), and a half maximal inhibitory concentration (IC₅₀) was determined. Malondialdehyde (MDA), lactate dehydrogenase (LDH) (spectrophotometry), mitochondrial depolarization (<i>Δψ</i>_m), intracellular reactive oxygen species (flow cytometry), glutathione (GSH), oxidized glutathione (GSSG) concentrations, caspase activities, ATP levels (luminometry), and fragmentation of DNA (SCGE assay) were evaluated. Protein expressions were assessed by western blotting. Gene expressions were quantified by qPCR. Cell viability in HEK293 cells was decreased in a dose-dependent manner by C3. MDA, <i>Δψ</i>_m, LDH, caspase activities, and DNA fragmentation (<i>P</i> < .0004) were significantly increased by C3. Nuclear factor erythroid 2-related factor 2 (Nrf-2) protein expression, GSH, and GSSG concentrations were increased, whereas antioxidant (<i>Nrf-2</i>, <i>GPx</i>, <i>SOD,</i> and <i>CAT</i>) gene expression was significantly decreased by C3 (<i>P</i> < .001). C3 decreased both Bax and Bcl-2 protein expression (<i>P</i> < .03). Gene expression of <i>c-myc</i> was significantly increased, whereas <i>OGG-1</i> was significantly reduced by C3 (<i>P</i> < .05). C3 reduced antioxidant gene expression, increased antioxidant levels, and elevated anti-proliferative effects in HEK293 cells, suggesting that high concentrations of C3 are potentially toxic to kidney cells, thus rendering cause for concern with its human use.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"455-464"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Strawberry (<i>Fragaria x Ananassa</i> Duch.) Mitigates Renal Fibrosis in a Unilateral Ureteral Obstruction Model by Reducing Inflammation, Oxidative Stress, and Regulating Smad Signaling.","authors":"Chia-Chu Chang, Erl-Shyh Kao, Yi-Chen Lee, Huei-Jane Lee","doi":"10.1089/jmf.2024.k.0257","DOIUrl":"https://doi.org/10.1089/jmf.2024.k.0257","url":null,"abstract":"<p><p>Renal fibrosis is a common outcome in many progressive renal diseases. Unilateral ureteral obstruction (UUO) is known to induce oxidative stress and inflammation in the kidneys, leading to the development of renal fibrosis. Fermented strawberry (<i>Fragaria x ananassa Duch.</i>) possesses antioxidant properties; however, its effect on renal fibrosis remains unclear. This study aimed to evaluate the impact of fermented strawberry dry powder (FSP) on renal fibrosis by assessing proinflammatory cytokines, oxidative stress markers, and underlying mechanisms. Male Sprague-Dawley rats were subjected to UUO surgery to induce tubulointerstitial fibrosis and obstructive nephropathy. Ten days postsurgery, the rats were randomly divided into four groups (<i>n</i> = 6), including a sham-operated control group. FSP was administered orally at doses of 0.05 or 0.5 g kg^-1 body weight daily for 21 days. FSP treatment significantly improved renal function, reduced tubular dilation, and decreased interstitial volume in UUO rats. FSP decreased levels of tumor necrosis factor-α and interleukin-6, while enhancing the activities of antioxidant enzymes such as superoxide dismutase and catalase. Treatment with 0.05 and 0.5 g kg^-1 FSP resulted in a reduction of collagen deposition in the kidneys by 49% and 69%, respectively, compared with UUO group. FSP increased E-cadherin expression and decreased α-smooth muscle actin level in the kidneys of UUO rats. Furthermore, FSP reduced transforming growth factor-β and Smad2/3 levels while upregulating Smad7 expression. These findings suggest that FSP mitigates renal tubulointerstitial fibrosis, likely through modulation of Smad signaling and attenuation of oxidative stress and inflammation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":"28 5","pages":"423-434"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Properties of Murici (<i>Byrsonima crassifolia</i>) and Bacuri (<i>Platonia insignis</i>) and Their Antioxidant and Anti-Inflammatory Effects in Rats.","authors":"Raquel Martins Martinez, Audrey Cristina Cintra, Carolina de Oliveira Ramos Petra de Almeida, Thuane Passos Barbosa Lima, Isabelle Moraes de Souza, Matheus Augusto Patrício de Almeida, Stephan Pinheiro Frankenfeld, Fabio Luiz Cândido Cahuê, Gabriela de Queiroz Pereira Lopes, Verônica Salerno Pinto, Adriana Ribeiro Silva, Cassiano Felippe Gonçalves-de-Albuquerque, Manuela Dolisnky, Anderson Junger Teodoro","doi":"10.1089/jmf.2023.0029","DOIUrl":"https://doi.org/10.1089/jmf.2023.0029","url":null,"abstract":"<p><p>Fruit consumption favors energy balance, ensures essential nutrients, and provides bioactive compounds with antioxidant and anti-inflammatory activities capable of preventing and treating noncommunicable diseases. Tremendous Brazilian biodiversity favors countless fruit and vegetable crops, including exotic fruits. This study aimed to describe the nutritional characteristics of murici and bacuri and their effects on oxidative stress and inflammation. The murici lyophilized extract (MLE) and bacuri lyophilized extract (BLE) were evaluated for their antioxidant activity and total phenolic compounds content. Healthy female Wistar rats were divided into control and supplemented groups (50, 100, and 250 mg/kg body weight) for each fruit, with a 14-day supplementation. Parameters of oxidative damage and antioxidant system in plasma and inflammatory biomarkers in the kidney and liver tissues were evaluated. Murici has carotenoids as the main bioactive compound, whereas bacuri is a source of polyphenols, with relevant antioxidant activity for human health. Treatment with MLE in healthy rats resulted in increased plasma antioxidant capacity measured by DPPH assay, reduced plasma levels of lipid peroxidation (TBARS) and carbonylated proteins, and lower TNF-α production in the kidney and liver. BLE supplementation reduced plasma lipid peroxidation levels and led to lower activity of IL-1 β, IL-6, and TNF-α in supplemented animals' kidneys. Both fruits showed positive antioxidant and anti-inflammatory effects against oxidative stress and inflammation in an <i>in vivo</i> model. The results point to new antioxidant and anti-inflammatory resources to be studied.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":"28 5","pages":"478-489"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of the Effects of Flavonoids on NAD(P)H Quinone Oxidoreductase 1 Expression and Activity.","authors":"Erik B Froyen, Gianluis Pimentel Barrantes","doi":"10.1089/jmf.2023.0132","DOIUrl":"10.1089/jmf.2023.0132","url":null,"abstract":"<p><p>Cancer is a significant cause of death worldwide. It has been suggested that the consumption of flavonoids decreases the risk for cancer by increasing phase II enzymes, such as Nicotinamide Adenine Dinucleotide Phosphate Hydrogen (NAD(P)H) quinone oxidoreductase 1 (NQO1), glutathione S-transferases, and Uridine 5'-diphospho- (UDP)-glucuronosyltransferases that assist in removing carcinogens from the human body. Flavonoids are bioactive compounds found in a variety of dietary sources, including fruits, vegetables, legumes, nuts, and teas. As such, it is important to investigate which flavonoids are involved in the metabolism of carcinogens to help reduce the risk of cancer. Therefore, the objective of this narrative review was to investigate the effects of commonly consumed flavonoids on NQO1 mRNA expression, protein, and activity in human cell and murine models. PubMed was used to search for peer-reviewed journal articles, which demonstrated that selected flavonoids (<i>e.g.</i>, quercetin, apigenin, luteolin, genistein, and daidzein) increase NQO1, and therefore, increase the excretion of carcinogens. However, more research is needed regarding the mechanisms by which flavonoids induce NQO1. Furthermore, it is suggested that future efforts focus on providing precise flavonoid recommendations to decrease the risk factors for chronic diseases.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"407-422"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laxative Effect of <i>Crataegus azarolus</i> Leaves Decoction Extract Against Loperamide-Induced Constipation and Oxidative Stress in Rats.","authors":"Houcem Sammari, Anouar Abidi, Saber Jedidi, Nourhène Dhawefi, Hichem Sebai","doi":"10.1089/jmf.2023.0255","DOIUrl":"10.1089/jmf.2023.0255","url":null,"abstract":"<p><p>Constipation represents a common gastrointestinal disorder that has various adverse effects on the gastrointestinal tract. As a result, various civilizations have developed phytomedicines in order to treat and relieve its symptoms. In the current study, we evaluated the effect of <i>Crataegus azarolus</i> L. leaves decoction extract (CALDE) against loperamide (LOP)-induced constipation in rats. For 3 days, treated rats were administered LOP (3 mg/kg, <i>b.w., p.o</i>.) and CALDE (50, 100, and 200 mg/kg, <i>b.w., p.o.</i>) or yohimbine (2 mg/kg, <i>b.w., p.o</i>.). The gastric emptying test or intestinal transit time was calculated. The oxidative status was studied and evaluated using biochemical colorimetric methods. Results showed that CALDE administration improves gastric emptying and accelerates gastrointestinal transit. Pretreatment with LOP altered the defecation parameters and generated an oxidative status in healthy rats. In contrast, CALDE coadministration protected against the deregulation of intestinal motor function and frequency of defecation and significantly re-established oxidative marker levels. CALDE treatment demonstrated significant protection against experimental oxidative stress and constipation induced by LOP. Therefore, it can be considered a pharmacological drug to treat these gastrointestinal troubles.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"465-477"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent Versus Continuous Wild Blueberry Feeding Alters Inflammation and Behavior in Aged Male Rats.","authors":"Barbara Shukitt-Hale, Derek R Fisher, Danielle S Cahoon, Marshall G Miller, Amanda N Carey, Tong Zheng","doi":"10.1089/jmf.2025.0001","DOIUrl":"https://doi.org/10.1089/jmf.2025.0001","url":null,"abstract":"<p><p>Previous studies have shown that continuous daily consumption of polyphenolic-rich blueberries (BB) reduced neuroinflammation and improved age-related declines in motor and cognitive function in rodents and humans, but the optimal intake of BBs is currently unknown. To evaluate the beneficial effects of BBs on neuroinflammation and motor and cognitive function relative to the frequency of consumption, the diets of aged F344 rats (18 months; <i>n</i> = 15/group) were supplemented with either a continuous control diet, a continuous 2% wild BB diet, or an intermittent (3 days on, 4 days off) 2% wild BB diet for 2 months prior to behavioral testing. Because neuroinflammation has been identified as a major contributing factor to the behavioral declines seen in aging and neurodegenerative diseases, serum was collected pre-diet and at the end of the study to assess whether diet-induced changes in serum can alter the production of inflammatory stress signals in activated HAPI rat microglial cells <i>in vitro</i>. Serum from rats continuously and intermittently fed BBs had beneficial effects by reducing the expression of inflammatory enzymes cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in microglia, but only continuous feeding attenuated nitrite production. Intermittent BB-fed rats performed better on the rotarod and committed fewer errors in the radial arm water maze on Day 2 compared to control-fed animals. Based on these findings, BBs may not have to be consumed daily to elicit beneficial effects, possibly due to the retention of polyphenols and their metabolites in tissue and circulation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The <i>In Vitro</i> and <i>In Silico</i> Assessment of the Cytotoxic Effect of Daidzein Alone or in Combination with 5-Fluorouracil on Human Colorectal Adenocarcinoma Cell Line Caco-2.","authors":"Altinay Altinkaynak, Orhan Kocak, Esra Tanyel Akcit, Ece Simsek, Kubra Yildirim, Ahmet Yilmaz Coban","doi":"10.1089/jmf.2024.0132","DOIUrl":"https://doi.org/10.1089/jmf.2024.0132","url":null,"abstract":"<p><p>We aimed to evaluate the cytotoxic effect of daidzein alone and in combination with 5-fluorouracil (5-FU) on Caco-2 cells and investigate potential interactions between daidzein and 5-FU with apoptosis-related tumor necrosis factor-related apoptosis-inducing ligand receptor-2, tumor necrosis factor -alpha receptor-1 and Interferon gamma receptor-1. In this study, we used trypan blue and MTT assays to evaluate the cytotoxic effect of daidzein and the effect of daidzein on the amount of substance P released from the cells using the ELISA method. We utilized the ligand-protein molecular docking method for our <i>in-silico</i> assessments. According to our cytotoxicity experiment results, daidzein decreased the proliferation of Caco-2 cells. It should be pointed out that our ELISA test results daidzein and 5-FU increase the level of Substance-P (SP), a proinflammatory cytokine released by Caco-2 cells. Based on our <i>in-silico</i> results, daidzein interacted with all three apoptosis-related receptors on the membrane of Caco-2 cells to a better extent than 5-FU. In sum, although daidzein increases the amount of SP, it is a compound that has significant cytotoxic effect on colon cancer cells and promise as a potential therapeutic agent for the treatment of colon cancer.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Curative Effects of Vitamins D3&K2 on the Expressions of p53, Bax, Bcl-2, and Oxidative Stress Induced by Methotrexate in ZFL Epithelial Cells.","authors":"Güllü Kaymak","doi":"10.1089/jmf.2024.0261","DOIUrl":"https://doi.org/10.1089/jmf.2024.0261","url":null,"abstract":"<p><p>Methotrexate (MTX), a folate antimetabolite, is a cytotoxic drug known to cause cytotoxicity associated with free oxygen radicals. This study investigated the effect of vitamins D3 and K2 on MTX-induced liver cell injury using the zebrafish liver cell line (CRL-2643). Observed effects, levels of antioxidant enzymes, lipid peroxidation marker, and total antioxidant/oxidant status were evaluated by spectrophotometric methods. The mRNA expressions of p53, Bax, and Bcl-2 were measured using RT-PCR. In addition, acridine orange/ethidium bromide staining was performed to analyze the apoptosis status of the cells. The IC₅₀ value of MTX at 48 h was calculated as 442 <i>µ</i>g/mL with an MTT assay. The doses of D3&K2 are determined based on the recommended dose to be taken daily. As a result, this study suggests that MTX treatment induced oxidative damage on the liver cell, as assessed by increased reactive oxygen levels, lipid peroxidation, and decreased glutathione levels. In addition, while MTX increased the expression of the p53 and proapoptotic marker Bax, it decreased the expression of the anti-apoptotic factor Bcl-2. In conclusion, D3&K2 treatment protects against MTX-induced liver cell toxicity. It is thought that they can be used as a potential agent in clinical applications with MTX in treatment.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}