Journal of medicinal food最新文献

{"title":"<i>Craibiodendron Stellatum</i> (Pierre) WW Sm. Extract Suppresses Lipopolysaccharide-Induced Acute Lung Injury by Regulating Macrophage-Mediated Inflammation <i>In Vivo</i> and <i>In Vitro</i>.","authors":"Da Hye Gu, Min Jeong Kim, Ardina Nur Fauziah, Sung Keun Jung","doi":"10.1177/1096620X251360148","DOIUrl":"https://doi.org/10.1177/1096620X251360148","url":null,"abstract":"<p><p>Acute lung injury (ALI), primarily triggered by bacterial infection, occurs via intricate immune defense mechanisms within the lungs. This study aimed to investigate the potential of <i>Craibiodendron stellatum</i> (Pierre) WW Sm. extract (CSE) as an anti-inflammatory agent in a lipopolysaccharide (LPS)-induced C57BL/6 mouse ALI model and RAW 264.7 macrophages. Results showed that oral CSE administration significantly suppressed LPS-induced ALI symptoms, including hemorrhage in alveolar spaces, thickened alveolar walls, and inflammatory cell infiltration; it also inhibited the production of proinflammatory cytokines in the lungs. Additionally, oral CSE administration attenuated the LPS-induced iNOS expression in mouse lungs. CSE also inhibited the LPS-induced nuclear factor-kappa B signaling in RAW 264.7 cells. DCF-DA assay confirmed that CSE could reduce the LPS-induced reactive oxygen species production. Our findings suggested that CSE may be useful as a functional respiratory health material by regulating the inflammatory signaling pathway in alveolar macrophages in ALI induced by bacterial infections.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Açai Seed Extract (<i>Euterpe oleracea</i> Mart.) Combined with Exercise Training on Cardiovascular Alterations, Oxidative Stress, and Loss of Physical Performance in Spontaneously Hypertensive Rats.","authors":"Beatriz Cardoso de Oliveira, Ricardo de Andrade Soares, Matheus Pontes de Menezes, Mariana Alencar Cavalheira, Dafne Lopes Beserra da Silva, Graziele Freitas de Bem, Dayane Teixeira Ognibene, Cristiane Aguiar da Costa, Roberto Soares de Moura, Angela Castro Resende","doi":"10.1177/1096620X251361429","DOIUrl":"https://doi.org/10.1177/1096620X251361429","url":null,"abstract":"<p><p>Hypertension increases the risk of mortality from cardiovascular complications. The objective of this study was to evaluate whether <i>Euterpe oleracea</i> Mart. (açaí) seed extract (ASE), a polyphenol-rich Amazonian plant, and moderate exercise training (TR), in combination or not, exert beneficial effects on cardiovascular structural and functional changes, oxidative stress, and loss of physical performance (PP) in spontaneously hypertensive rats (SHR). Five groups were assigned: Control (CT), SHR, SHR + ASE (200 mg/kg/day by gavage), SHR + TR, and SHR + TR + ASE. The TR was performed on a treadmill for 8 weeks (5×/week) for 30 min. Blood pressure, cholesterol, and triglyceride levels were measured. Vascular reactivity was evaluated in the mesenteric arterial bed (MAB) and aortic ring. Aorta samples were obtained for biochemical, immunohistochemical, and morphological assessments. Running distance and exercise time increased in SHR + TR compared with the first maximal stress test. This performance was lost in the third test but restored with ASE. Hypertension, aorta hypertrophy, reduced acetylcholine-induced vasodilation, phosphorylated endothelial nitric oxide synthase (p-eNOS) expression, oxidative damage (3,4-methylenedioxyamphetamine), and superoxide dismutase activity were improved by ASE but not TR. ASE and TR alone improved endothelial dysfunction in MAB and fibrosis in the aorta. The lipid profile and glutathione peroxidase activity improvement were observed only in SHR + TR + ASE, and additional p-eNOS expression and anti-hypertrophy effect were observed. In conclusion, ASE was superior to TR as an antihypertensive strategy because it improved vascular endothelial dysfunction, hypertrophy, and oxidative stress in SHR. The association of both strategies further improves vascular hypertrophy, antioxidant defense, the loss of PP, and lipid profile, which may benefit hypertension-related cardiovascular risks.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Eriobotrya japonica</i> Leaf Extract Ameliorates Benign Prostatic Hyperplasia by Regulating Inflammation.","authors":"Hyung-Gu Son, Jeong Yoon Lee, Heeju Kim, Young-Mi Cho, Yoo-Hyun Lee","doi":"10.1177/1096620X251360539","DOIUrl":"https://doi.org/10.1177/1096620X251360539","url":null,"abstract":"<p><p><i>Eriobotrya japonica</i>, a Rosaceae family plant, is cultivated in East Asia and possesses various biological activities. It has a long history of use in traditional medicine practices. Benign prostatic hyperplasia (BPH) is a common urological issue in aging men. Although its exact etiology remains unidentified, it is believed to be caused by a combination of factors such as aging, inflammation, and hormonal imbalance. This study examined the effects of <i>E. japonica</i> leaf 50% ethanol extracts (EJE) on human prostate epithelial BPH-1 cells and a BPH rat model developed <i>via</i> testosterone propionate (TP) injection. In BPH-1 cells, EJE treatment suppressed the proliferation of the cells in a dose-response relationship. In addition, mRNA expression levels of androgen receptor (AR), steroid 5 alpha-reductase type 2 (SRD5A2), and Bcl-2 were decreased, and Bax expression and the Bax/Bcl-2 ratio were increased by EJE treatment. In the <i>in vivo</i> experiment, castrated Sprague-Dawley (SD) rats were orally administered EJE at the following doses: 50-200 mg/kg of body mass. EJE administration reduced prostate weight, prostate ratio, serum dihydrotestosterone (DHT) levels, and serum testosterone levels. It also decreased intraprostatic DHT levels and 5<i>α</i>-reductase 2 expression. Histological analysis revealed a reduced epithelial thickness and increased lumen area in the EJE treatment group. Furthermore, the expression of AR, Bcl-2, tumor necrosis factor (TNF)-<i>α</i>, interleukin (IL)-8, IL-6, IL-1<i>β,</i> and cyclooxygenase-2 (COX-2) in prostate tissues was lowered by EJE administration. In conclusion, EJE effectively alleviated benign prostatic hyperplasia, both in cells and in animal models, by regulating inflammation, suggesting it is a potential candidate as a functional ingredient for alleviating BPH.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taurine Alleviates the Number of Nuclear Apoptotic Hepatocytes Induced by Cyclosporine A in Rat Liver.","authors":"Mona A H Yehia, Kawther M Foud, Hanan A El Sayed, Hagar M Mohamed, Ahmad M Attia","doi":"10.1177/1096620X251359940","DOIUrl":"https://doi.org/10.1177/1096620X251359940","url":null,"abstract":"<p><p>Cyclosporine A (CsA) is a powerful immunosuppressant drug most widely used in managing organ transplantation and autoimmune diseases. The aim of this work was to investigate the role of taurine in alleviating the apoptotic hepatocytes and oxidative stress caused by CsA in rats' livers. The four experimental groups were evaluated, including (GpI) vehicle control (olive oil), (GpII) Tau (5 mg/kg/day), (GpIII) CsA (50 mg/kg/day), and (GpIV) CsA + Tau. The biochemical assay in liver functions and antioxidant enzymes was assayed, and the histopathology of hepatic tissue and immunohistochemical staining of apoptotic protein (p53) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were determined. Induction of CsA in rats caused severe hepatotoxicity, as evidenced by the elevation of serum aspartate aminotransferase, alanine aminotransferase activities, and alkaline phosphatase concentration and decreased catalase (CAT), glutathione peroxidase, and glutathione reductase activities. The histopathological examination revealed mild to marked disorganization in the liver tissue, characterized by hepatocyte degeneration/necrosis, apoptotic hepatocytes, sinusoidal dilatation, and inflammatory cell infiltration. Whereas Tau treatment improved the liver function enzymes and increased the oxidative stress by elevating the antioxidant enzyme CAT and glutathione reductase. There is recovery of destructive liver tissue preserved hepatic trabecular architecture; dark nuclei with prominent nucleoli, hepatocytes, and mild dilated sinusoids; small area of pyknotic hepatocytes have vacuolated cytoplasm was seen, and the number of apoptotic cells detected by TUNEL and p53 protein was significantly decreased (<i>P</i> = .001). The results may contribute to the hepatoprotective role of Tau and its ability to ameliorate the oxidative stress and alleviate the apoptotic hepatocytes induced by CsA. So, Tau may have had a beneficial role in reducing tissue damage in patients exposed to CsA.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Microencapsulated and Nonencapsulated Aronia Extract on Paraoxonase 1 Gene Expression and Aortic Histopathology in High-Fat Diet-Fed Rats.","authors":"Serim Tuna Koç, Süleyman Kök, Sertaç Atalay, Onur Ersoy","doi":"10.1089/jmf.2025.0027","DOIUrl":"https://doi.org/10.1089/jmf.2025.0027","url":null,"abstract":"<p><p>The aim of this study was to determine the effects of microencapsulated and nonencapsulated aronia (<i>Aronia melanocarpa</i>) extract on paraoxonase 1 (PON1) mRNA expression, HDL cholesterol, and aortic atherosclerosis in rat blood and liver tissues. The study involved 42 male Sprague-Dawley rats aged 10 weeks. The experimental groups were as follows: (1) standard diet control (CON), (2) high-fat diet (HF) control, (3) HF + 400 mg/kg aronia extract (HF400E), (4) HF + 200 mg/kg aronia extract (HF200E), (5) HF + 400 mg/kg microencapsulated aronia (HF400C), and (6) HF + 200 mg/kg microencapsulated aronia (HF200C). The rats were fed a HF between the 10th and 20th week, and at the end of the 20th week, all groups except CON were administered aronia extract (200-400 mg/kg) by oral gavage for 6 weeks. After 6 weeks, biochemical analyses and aortic histology evaluations were performed. The results showed that the HF significantly increased serum total cholesterol, triglycerides, low-density lipoprotein levels, and hepatic malondialdehyde levels. However, administration of HF400E significantly reduced these parameters in hyperlipidemic rats. Serum PON1 enzyme activity was significantly higher in the HF400E group compared with both the CON (<i>P</i> < .001) and HF (<i>P</i> < .0025) groups. Although PON1 mRNA levels in the HF400E, HF200E, and HF200C groups were higher than in the CON and HF groups, the differences were not statistically significant. Evaluation of the aortic tissues showed normal tissue morphology in all experimental groups, with no signs of atherosclerotic plaques, hyaline or mucoid changes, or endothelial anomalies in the thoracic or aortic arch tissues. These findings suggest that aronia, rich in anthocyanins and other polyphenolic compounds, may help prevent and slow down atherosclerosis by enhancing PON1 enzyme activity, which is involved in preventing lipid oxidation, the first step in atherosclerosis.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Crataegus songarica</i> Fruit Ameliorates Oxidative Stress, Hypertension, and Endothelial Dysfunction in Glucose-Induced Hypertensive Rats.","authors":"Waqas Younis, Alamgeer Alamgeer, Muhammad Nasir Hayat Malik, Tariq G Alsahli, Wajiha Manzoor, Muhammad Majid, Malik Zahid Imran, Muryam Abdul Razzaq, Alana Anne Kaneda Garcia, Emerson Luiz Botelho Lourenço, Aline Aparecida Macedo Marques, Arquimedes Gasparotto Junior","doi":"10.1089/jmf.2023.0115","DOIUrl":"https://doi.org/10.1089/jmf.2023.0115","url":null,"abstract":"<p><p><i>Crataegus songarica</i> is a native species of Pakistan. Its fruits are locally consumed for the treatment of various cardiovascular disorders, including hypertension, heart failure, and vascular insufficiency. Despite its traditional use, data regarding its effectiveness remain unclear. Therefore, the aim of the study was to evaluate the cardioprotective effects of <i>C. songarica</i> fruit extract on glucose-induced hypertensive rats. First, the aqueous-methanol extract (AMECS) was obtained and subjected to phytochemical characterization. The antioxidant and cytotoxic properties were investigated <i>in vitro</i>. Acute toxicity as well as screening for hypotensive effects was also evaluated. The cardioprotective effects were assessed in glucose-induced hypertensive rats after 21 days of treatment with AMECS (500 mg/kg single-daily oral dose). At the conclusion of the treatments, we investigated hemodynamic and biochemical parameters as well as endothelium-dependent vascular reactivity and tissue redox state. AMECS exhibited a significant antioxidant effect <i>in vitro</i>. In addition, no evidence of acute toxicity was observed. In glucose-induced hypertensive rats, AMECS prevented the increase in systolic blood pressure, oxidative stress, endothelial dysfunction, and metabolic alterations induced by glucose. The data obtained led us to conclude that the fruit extract of <i>C. songarica</i> presents a significant cardioprotective effect in glucose-induced hypertensive rats.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic Effects and Underlying Mechanisms of Food-Derived Bioactive Peptides.","authors":"Hatice Ozcaliskan Ilkay, Gulhan Samur","doi":"10.1089/jmf.2024.0018","DOIUrl":"https://doi.org/10.1089/jmf.2024.0018","url":null,"abstract":"<p><p>Type 2 diabetes mellitus (T2DM), characterized by progressive insulin secretion defect based on insulin resistance, is one of the leading public health problems with high morbidity and mortality rates. Uncontrolled hyperglycemia, which becomes a more severe indication with obesity in T2DM patients, increases the risk of cardiovascular disease, kidney damage, and retinal disorder. Effective diabetes treatment is possible with a comprehensive approach that includes controlling blood glucose levels, improving pancreatic β cell functions, and supporting insulin sensitivity through body weight management, nutritional therapy, and drug therapy. At this point, food-derived bioactive peptides and protein hydrolysates, which draw attention with their structural similarity to regulatory peptides in human metabolism, have great potential in treating T2DM and regulating glucose metabolism. Various glucoregulatory properties of bioactive peptides come to the fore through antidiabetic mechanisms such as the digestion of carbohydrates, the release of intestinal hormones, insulin function and secretion, glucose uptake, and adipose tissue modification. This review aims to evaluate the roles of food-derived bioactive peptides and protein hydrolysates in controlling glycemia and insulin sensitivity and their antidiabetic mechanisms of action and to examine the difficulties and opportunities related to the acquisition and research processes.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Oral Bioavailability of Lutein and Zeaxanthin via a Self-Emulsifying Delivery System: A Randomized, Double-Blind Cross-Over Study.","authors":"Byung-Sun Choe, Ambrish C, Priya Mk, Jinhak Kim, Kwang-Soo Baek, Yoo-Kyoung Park","doi":"10.1089/jmf.2025.k.0060","DOIUrl":"https://doi.org/10.1089/jmf.2025.k.0060","url":null,"abstract":"<p><p>This study was conducted to evaluate and verify the improved bioavailability, as determined by the plasma concentrations of lutein and zeaxanthin, of the test supplement, XanMax® 2002 plus LuZeAbility™, as compared to the reference supplement, XanMax® 2002. For this purpose, this study was designed as a randomized, double-blind, two-group, two-period cross-over clinical trial research. A total of 24 male subjects participated in the clinical trial. They were randomized 1:1 into group 1 or 2 to consume two types of supplements in two separate periods. This study aimed to propose and demonstrate that the bioavailability and the plasma concentrations of lutein and zeaxanthin in the test supplement were significantly higher (110-132.8%) than in the reference supplement in all consecutive periods, such as 12 to 72 h after intake and at the time of maximum concentration. These results are expected to strengthen macular pigment optical density levels, ultimately providing a safe and effective intervention for comprehensively promoting eye health. Therefore, the findings of this study have significant pharmacokinetic implications and offer valid theoretical and practical insights for both academic research and the industrial development in the supplement market.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ketogenic Diet for Epilepsy: The Olive Oil Effect to Optimization. A Narrative Review.","authors":"Sofia Zouganeli, Mary Yannakoulia, Achilleas Attilakos, Smaragdi Fessatou, Evdokia K Mitsou, Adamantini Kyriacou, Argirios Dinopoulos","doi":"10.1089/jmf.2025.0009","DOIUrl":"https://doi.org/10.1089/jmf.2025.0009","url":null,"abstract":"<p><p>The ketogenic diet (KD) has long been used in the treatment of drug-resistant epilepsy, demonstrating significant beneficial health effects. Various modifications of the KD have been introduced as alternatives to the classical, more restrictive form, aiming to improve patient's adherence while maintaining therapeutic efficacy. Among these adaptations, the Mediterranean ketogenic diet (MedKD) has been primarily implemented in populations in the Mediterranean region. The MedKD integrates elements of the traditional Mediterranean diet, with olive oil as the primary fat source, leveraging its unique properties. This narrative review examines the possible connections between olive oil and the mechanisms of KD, proposing the extra virgin olive oil-rich MedKD as a healthier option with the potential for reduced adverse effects.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Curative Effects of Vitamins D3&K2 on the Expressions of p53, Bax, Bcl-2, and Oxidative Stress Induced by Methotrexate in ZFL Epithelial Cells.","authors":"Güllü Kaymak","doi":"10.1089/jmf.2024.0261","DOIUrl":"10.1089/jmf.2024.0261","url":null,"abstract":"<p><p>Methotrexate (MTX), a folate antimetabolite, is a cytotoxic drug known to cause cytotoxicity associated with free oxygen radicals. This study investigated the effect of vitamins D3 and K2 on MTX-induced liver cell injury using the zebrafish liver cell line (CRL-2643). Observed effects, levels of antioxidant enzymes, lipid peroxidation marker, and total antioxidant/oxidant status were evaluated by spectrophotometric methods. The mRNA expressions of p53, Bax, and Bcl-2 were measured using RT-PCR. In addition, acridine orange/ethidium bromide staining was performed to analyze the apoptosis status of the cells. The IC₅₀ value of MTX at 48 h was calculated as 442 <i>µ</i>g/mL with an MTT assay. The doses of D3&K2 are determined based on the recommended dose to be taken daily. As a result, this study suggests that MTX treatment induced oxidative damage on the liver cell, as assessed by increased reactive oxygen levels, lipid peroxidation, and decreased glutathione levels. In addition, while MTX increased the expression of the p53 and proapoptotic marker Bax, it decreased the expression of the anti-apoptotic factor Bcl-2. In conclusion, D3&K2 treatment protects against MTX-induced liver cell toxicity. It is thought that they can be used as a potential agent in clinical applications with MTX in treatment.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"657-667"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}