Journal of medicinal food最新文献

{"title":"Biological Effects of Bovine Lactoferrin on Keratinocyte Proliferation and Migration (Bovine Lactoferrin and Wound Healing Response).","authors":"Morgana Lüdtke Azevedo, Laís Medeiros Cardoso, Caroline Anselmi, Rafael Guerra Lund","doi":"10.1177/1096620X261460635","DOIUrl":"10.1177/1096620X261460635","url":null,"abstract":"<p><p>Lactoferrin (Lf), an iron-binding glycoprotein present in various exocrine secretions, exhibits a wide range of biological activities, including cell proliferation, immune modulation, and antimicrobial effects. This study investigates the bioactivities of commercially available Lf derived from bovine milk. Commercial Lf, with a purity exceeding 95%, was analyzed using chemical and cell-based assays. Fourier-transform infrared spectroscopy confirmed the protein's identity through characteristic peaks. Cell viability assays demonstrated noncytotoxic effects, while cell morphology analysis and proliferation assays revealed that Lf stimulated human keratinocyte (HaCaT) proliferation in a concentration-dependent manner. Wound healing assays showed that Lf promotes wound closure, with optimal effects observed at lower concentrations. However, antimicrobial assays revealed that Lf alone did not exhibit activity against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. These findings highlight the importance of purity and concentration in determining the biological activity of Lf. Further studies are warranted to investigate the molecular mechanisms underlying these effects and the potential applications of bovine Lf in therapeutic contexts.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"655-664"},"PeriodicalIF":1.8,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot Study of Gallic Acid Cream as a Potential Treatment for Plaque Psoriasis.","authors":"Robert R Greenway, Ann A Coulter, Dachuan Zhang, Frank L Greenway","doi":"10.1177/1096620X261458593","DOIUrl":"10.1177/1096620X261458593","url":null,"abstract":"<p><p>Gallic acid, a catechin polyphenol in tea, completely inhibited angiogenesis in assays of human tissue at 10^-3 M. Psoriasis is a skin disease caused by excessive secretion of angiogenenic factors by keratinocytes and stromal skin cells. In a double-blind pilot study, six subjects with bilateral plaque psoriasis were treated with 10^-2 M gallic acid in a cream base or with a cream base placebo over 8 weeks, four times a day, and treatments were randomly assigned to either the left or the right side. The gallic acid cream was well tolerated, but it did not reduce the psoriasis more than the placebo. One subject wanted to extend treatment for an additional 8 weeks. At the end of 16 weeks, the subject had complete resolution of the psoriatic plaque treated with gallic acid cream. In contrast, the plaque treated with placebo cream was not reduced and stayed equivalent to week six. Gallic acid is inexpensive and does not cause side effects. A longer trial evaluating 10^-2 M gallic acid cream for the treatment of psoriasis seems indicated.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"637-642"},"PeriodicalIF":1.8,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardized <i>Artemisia argyi</i> Extract Alleviates Scopolamine-Induced Memory Impairment in Mice.","authors":"Seung Hyun Byun, Keontae Park, Somin Moon, Tae Ho Lee, Sang Ho Lee, Hai Hua Jiang, Eun Hye Han, Ju Eun Han, Seo Yun Jung, Dong Hyun Kim, Jong Hoon Ryu","doi":"10.1177/1096620X261462220","DOIUrl":"10.1177/1096620X261462220","url":null,"abstract":"<p><p>Cognitive dysfunction has become a major health issue in the global aging society. For treating cognitive dysfunction or dementia, several small molecules or innovative drugs, including a monoclonal antibody against the amyloid β protein, have been prescribed. However, their adverse effects require the exploration of alternative treatments. <i>Artemisia argyi</i> (Asteraceae) has been used as traditional medicine for gastrointestinal disorders and inflammation. Recent reports suggest it has potential neuroprotective effects; however, evidence regarding its cognitive benefits and underlying mechanisms remains limited. We examined the memory-enhancing effects of an ethanolic extract of <i>A</i>. <i>argyi</i> (EAA) (10, 30, or 100 mg/kg, once per each behavioral test) in a mouse model of scopolamine-induced cognitive dysfunction and explored its mode of action on memory-related signaling pathways. Memory-related performance was assessed using the Y-maze, novel object recognition, Morris water maze, and passive avoidance tests. Next, Western blotting was used to evaluate changes in signaling molecules, such as CaMKII, ERK, CREB, and brain-derived neurotrophic factor (BDNF) in the hippocampus. EAA administration significantly improved cognitive performance across all tests. It restored the phosphorylation levels of CaMKII, ERK, and CREB, respectively, as well as the expression level of BDNF, after 12 h of treatment. Moreover, it enhanced long-term potentiation without altering basal synaptic transmission within the hippocampus. In summary, EAA facilitated cognitive enhancement during a cholinergic impaired state, attributable to its influence on synaptic plasticity and activation of the CaMKII-ERK-CREB-BDNF signaling cascade.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"665-674"},"PeriodicalIF":1.8,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method Validation for Phenylalanine Analysis in Unconventional Food Plants.","authors":"Elaine Carvalho Minighin, Maria de Fátima Gomides, Mariem Rodrigues Ribeiro da Cunha, Lorena Thais Souza Dias, Karen Costa, Ana Luíza Santos Vieira, Marinalva Woods Pedrosa, Renata Adriana Labanca, Raquel Linhares Bello de Araújo","doi":"10.1177/1096620X261458997","DOIUrl":"10.1177/1096620X261458997","url":null,"abstract":"<p><p>Dietary restriction of high-protein foods is the primary treatment strategy for phenylketonuria, a disease considered an inborn error of metabolism associated with phenylalanine. The diet of these patients is monotonous, consisting mainly of certain types of fruits and vegetables, and is often supplemented with the use of phenylalanine-free formulas. Considering that Brazil is one of the countries with the most incredible biodiversity in the world, Non-Conventional Food Plants (NCPPs) become food options that can be regularly introduced into the diet of phenylketonuric patients. In the present study, a method for quantifying phenylalanine in NCPPs was validated using high-performance liquid chromatography with an ultraviolet detector. The phenylalanine content was determined in 15 NCPPs samples from the Brazilian Cerrado. The validated method demonstrated selectivity for the amino acid phenylalanine, with linearity in the range of 1-10 nmol/mL, indicating no matrix effect, and provided proven recovery, repeatability, and precision. The detection and quantification limits were 0.0011 mg/100 g and 0.0035 mg/100 g, respectively. The phenylalanine content ranged from 20.28 to 235.58 mg/100 g. It was observed that leafy vegetables had a higher phenylalanine content than the taro stem (20.28 mg/100 g), the chayote fruit (32.67 mg/100 g), the sepal of the sorrel plant (38.21 mg/100 g), and the nasturtium flower (116.24 mg/100 g). These data suggest that these vegetables can be incorporated into the diet of phenylketonuric patients, thereby contributing to dietary diversity and making it less monotonous and more palatable.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"643-654"},"PeriodicalIF":1.8,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 12-Week Randomized, Double-Blind, Placebo-Controlled Trial of <i>Salacia reticulata</i> Extract for Fat-Mass Reduction in Adults with Overweight/Obesity.","authors":"Seong-Hwan Park, Jung-Eun Lee, Sun-Gyu Bae, Jinhak Kim, Kwang-Soo Baek, Yuri Gwon, Sang-Jun Youn, Yong Choi, Yu-Jin Choi, Seon-Mi Shin","doi":"10.1177/1096620X261453229","DOIUrl":"10.1177/1096620X261453229","url":null,"abstract":"<p><p>Salacia reticulata has long been used in traditional medicine for metabolic disorders, but clinical evidence for obesity-related outcomes remains limited. We conducted a 12-week randomized, double-blind, placebo-controlled trial to evaluate the preliminary efficacy and safety of a standardized <i>Salacia reticulata</i> extract (SLE) on body-fat outcomes. Adults with overweight and/or obesity were randomized to receive SLE capsules or a matching placebo twice daily for 12 weeks. The coprimary endpoints were changes from baseline to week 12 in total body fat mass (g) and body fat percentage (%) measured by dual-energy X-ray absorptiometry. The primary efficacy analysis was conducted in the full analysis set (mITT) using analysis of covariance with baseline values and sex as covariates. Per-protocol set analyses were performed as supportive sensitivity analyses. Among randomized participants, the mITT included 66 participants in the SLE group and 67 in the placebo group, and baseline characteristics were comparable. After 12 weeks, total body fat mass decreased in the SLE group, whereas little change was observed in the placebo group. The between-group difference favored SLE (ANCOVA-adjusted LS mean difference, -482.30 g; 95% CI, -907.05 to -57.54 g). The corresponding between-group comparison was statistically significant (Wilcoxon rank-sum test, <i>P</i> = .0158). Body fat percentage also decreased in the SLE group, but the between-group difference was not statistically significant (ANCOVA-adjusted LS mean difference, -0.30%; 95% CI, -0.73 to 0.12%; Wilcoxon rank-sum test, <i>P</i> = .1453). Several secondary body-composition outcomes, including trunk, android and gynoid fat measures, showed directionally favorable changes in the SLE group. In this 12-week trial, SLE was associated with a modest reduction in total body fat mass, whereas the second coprimary endpoint, body fat percentage, did not differ significantly between groups. These mixed coprimary findings provide only preliminary clinical evidence and should be interpreted with caution, particularly given the study's retrospective trial registration.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"627-636"},"PeriodicalIF":1.8,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactobacillus delbrueckii</i> subsp. <i>lactis</i> CKDB001 Attenuates Hepatic Steatosis and Metabolic Dysfunction in High-Fat Diet-Induced Obese Mice.","authors":"Jisu Lee, Minseo Cho, Jaeryang Chu, Yeon-Woo Kim, Jiwoo Lee, Yoo Jin Kwon, Chang Hun Shin, Hyunchae Joung, Jung-Heun Ha","doi":"10.1177/1096620X261500242","DOIUrl":"https://doi.org/10.1177/1096620X261500242","url":null,"abstract":"<p><p>Metabolic dysfunction associated with obesity frequently coincides with hepatic lipid accumulation; however, effective preventive strategies are scarce. This study evaluated the dose-dependent effects of <i>Lactobacillus delbrueckii</i> subsp. <i>lactis</i> CKDB001 in mice with high-fat diet (HFD)-induced obesity. Male C57BL/6J mice were assigned to a normal diet, HFD, or HFD with CKDB001 at one of three different doses (LL1, 5.0 × 10⁸; LL2, 1.0 × 10⁹; LL3, 5.0 × 10⁹ CFU/day). Following 4 weeks of HFD feeding, CKDB001 was administered daily for 8 weeks while maintaining the HFD. Among the doses, LL2 demonstrated the most consistent outcomes, including reductions in body weight gain and liver and adipose tissue weights, without affecting food or energy intake. LL2 also decreased serum total cholesterol, alanine aminotransferase levels, hepatic cholesterol content, and histological scores for steatosis, fibrosis, and nonalcoholic fatty liver disease. Although changes in hepatic triglyceride and lipid metabolism-related gene expression were minimal, LL2 and LL3 enhanced colonic <i>Zo-1</i> and <i>occludin</i> mRNA expression compared to the HFD group, suggesting a potential gut-associated transcriptional response to CKDB001 supplementation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"1096620X261500242"},"PeriodicalIF":1.8,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Walnuts Modulate Cognition, Inflammation, and BDNF Expression in Rats.","authors":"Amanda N Carey, Shibu M Poulose, Derek R Fisher, Donna F Bielinski, Barbara Shukitt-Hale","doi":"10.1177/1096620X261495718","DOIUrl":"https://doi.org/10.1177/1096620X261495718","url":null,"abstract":"<p><p>Aging is associated with increased neuroinflammation and a decrease in neuroplasticity, contributing to cognitive decline and increased risk for neurodegenerative disease. Nutritional interventions, such as walnut supplementation, may counteract these effects due to walnuts' high content of polyunsaturated fatty acids, polyphenols, and other bioactive compounds. Prior studies suggest walnuts improve cognitive function and modulate neuroplasticity-, inflammatory-, and oxidative stress-associated pathways in both animal models and humans. We investigated the effects of 8 weeks of dietary walnut supplementation (0%, 6%, or 9% w/w) on behavioral and molecular markers of brain aging in young and aged Fischer 344 rats. Cognitive performance was assessed with the radial arm water maze (RAWM). To examine potential anti-inflammatory mechanisms, we treated lipopolysaccharide (LPS)-activated BV-2 microglial cells with serum collected from the rats pre- and post-diet and quantified nitric oxide (NO) release and inducible nitric oxide synthase (iNOS) expression. Additionally, gene expression of brain-derived neurotrophic factor (BDNF) was evaluated in the hippocampus and frontal cortex tissues. Our results demonstrated that walnut supplementation improved performance by decreasing the number of errors made in the RAWM. Serum from walnut-fed rats significantly reduced NO release and iNOS expression in LPS-stimulated microglia. Hippocampal BDNF mRNA expression also differed according to age and diet, with higher expression in aged than young rats receiving the 9% walnut diet. These findings suggest that dietary walnuts may support healthy brain aging through effects on cognitive performance and circulating factors that modulate inflammatory responses and BDNF expression.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"1096620X261495718"},"PeriodicalIF":1.8,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melphalan's Effect on Testicular Prepubertal Rats: Induction of Autophagy and Apoptosis, Modulated by the Potential of Chrysin.","authors":"Amir-Hossein Ebadi, Maryam Moghimian, Seyed-Hossein Abtahi-Evari, Zahra Saadatian, Seyed-Hasan Hosseini, Malihe Soltani","doi":"10.1177/1096620X261434448","DOIUrl":"10.1177/1096620X261434448","url":null,"abstract":"<p><p>Melphalan, a widely used alkylating chemotherapeutic agent, is known to cause gonadotoxicity, particularly affecting spermatogenesis and steroidogenesis. This study investigated the protective effects of chrysin, a flavonoid with antioxidant and antiapoptotic properties, on melphalan-induced testicular damage in immature male rats. Forty-eight prepubertal Wistar rats were divided into six groups receiving melphalan (0.5 mg/kg/day, intraperitoneally), chrysin (50 or 75 mg/kg/day, orally), or combinations thereof. Treatment spanned 30 days, covering the prepubertal to pubertal transition. Melphalan exposure significantly reduced testosterone levels, disrupted spermatogenesis, and increased oxidative stress, as indicated by elevated malondialdehyde and decreased glutathione peroxidase levels. Gene expression analysis revealed marked upregulation of autophagy (LC3β, Beclin-1, Atg5, Atg7, Atg12) and apoptosis (BAX, caspase-3) markers, alongside downregulation of the antiapoptotic gene BCL2. Histological analysis showed severe damage to seminiferous tubules and reduced Johnsen scores. Chrysin coadministration, particularly at 75 mg/kg, significantly ameliorated these effects by restoring antioxidant capacity, reducing expression of cell death-related genes, and improving testicular structure and hormone levels. These findings demonstrate that chrysin mitigates melphalan-induced gonadotoxicity through modulation of oxidative stress, apoptosis, and autophagy pathways. Chrysin's dose-dependent protective effects highlight its potential as a natural therapeutic agent to preserve reproductive function in individuals undergoing chemotherapy during prepuberty.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"587-593"},"PeriodicalIF":1.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Lactiplantibacillus plantarum</i> KABP051 Probiotic on Body Composition, Microbiome and Mood in Healthy Overweight Adults.","authors":"Shawn Talbott, Bret Stephens, Julie Talbott, Marc Oddou, Aoki Fumiki","doi":"10.1177/1096620X261448041","DOIUrl":"10.1177/1096620X261448041","url":null,"abstract":"<p><p>Obesity and mental health disorders are among the greatest public health challenges of the 21st century. Interestingly, an altered microbiome profile has been associated with both conditions. The aim of this randomized, double-blind, placebo-controlled clinical trial was to evaluate the effects of dietary supplementation with a specific probiotic strain (<i>Lactiplantibacillus plantarum</i> KABP051) on body composition and gut microbiome balance, together with measures of mood state, in a population of healthy overweight subjects. Sixty healthy, moderately stressed, nondepressed and overweight or obese volunteers were supplemented for 12 weeks with probiotic (<i>L. plantarum</i> KABP051; 1 billion colony forming units/day) or placebo (microcrystalline cellulose). The KABP051 group experienced significantly greater improvements compared with placebo on body composition measurements, including a reduction in body weight and waist circumference, which decreased in 1.97 ± 0.77 (mean ± SE) kg and 2.15 ± 0.81 (mean ± SE) cm versus placebo at the end of the intervention (both <i>P</i> < .05, mixed model for repeated measures [MMRM] and <i>post-hoc</i> analysis). Microbiome composition improved in KABP051 group, with significant increase in the relative abundance of <i>Lactiplantibacillus</i> spp. versus placebo. Body fat percentage, profile of mood states fatigue, and confusion sub-scores showed a global trend toward improvement compared with placebo, with the change at 12 weeks being significant in the three measurements in <i>post-hoc</i> analysis (<i>P</i> = .015, <i>P</i> = .014, and <i>P</i> = .016, respectively). No serious adverse events were registered during the intervention period. These results suggest that a specific strain of probiotic bacteria (<i>L. plantarum</i> KABP051) may have both metabolic and psychobiotic effects and may be beneficial for enhancing weight loss and body composition, improving energy (less fatigue) and mood levels while embarking on a healthy lifestyle regimen. ClinicalTrials.gov identifier: NCT06808061.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"603-610"},"PeriodicalIF":1.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Antioxidant and Anti-Inflammatory Response in the Eyes of CKD Animals Supplemented with Larch Arabinogalactan.","authors":"Pedro Henrique Alves Reis, Isabela de Paula Destro, Ingrid Bertollini Lamy, Giuliana Petri, Estella Freitas Silvestri, Isabella Dudjak Rosa Trufelli, Beatriz da Costa Aguiar Alves, Vagner Loduca Lima, Glaucia Luciano da Veiga, Fernando Luiz Affonso Fonseca","doi":"10.1177/1096620X261447993","DOIUrl":"10.1177/1096620X261447993","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) is a systemic condition associated with inflammation and oxidative stress, affecting organs beyond the kidneys. Although rarely emphasized, the eyes may also be affected but underlying molecular mechanisms remain largely unexplored. The gut-kidney and gut-eye axes are emerging as therapeutic targets with prebiotics like ResistAid®-a Larch Arabinogalactan (LAG) supplement with antioxidant and immunomodulatory effects-showing promise through gut microbiota modulation. This study assessed ResistAid®'s effects on ocular gene expression in a CKD rat model. Twenty four Wistar rats were assigned to Sham (S), Sham + Treatment (ST), Nephrectomized (N), Nephrectomized + Treatment (NT) (<i>n</i> = 6 each). CKD was induced by 5/6 nephrectomy. The treatment was administered via gavage for 30 days at a dose of 5.35 mg/day, adapted from human recommendations. At day 30, blood and tissues were collected. Expression of antioxidant enzymes (<i>Cat, Sod1, Gpx1, Gpx4</i>) and other genes (<i>Nfe2l2, Nfκb1, Timp3, Hif-1α, Kim1, Klotho</i>) was analyzed by qPCR. Biochemical and well-being assessments were also conducted. Nephrectomy, regardless of treatment, increased <i>Sod1</i> and <i>Hif1ɑ</i> expression in eye and blood; Specific to NT animals, ocular <i>Gpx1</i>, <i>Gpx4</i> and <i>Nfe2l2</i> expressions were markedly elevated when compared with N animals and blood <i>Kim1</i> and ocular <i>Nfκb1</i> expressions were not elevated, differing from N animals. No significant changes were observed between the S and ST groups. CKD induces systemic oxidative and inflammatory responses. ResistAid® partially mitigated these effects in blood and eye, suggesting systemic and local benefits, possibly via gut microbiota modulation.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"594-602"},"PeriodicalIF":1.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}