Journal of Molecular Cell Biology最新文献

Probing centromere-kinetochore core complex CENP-L/M assembly using cenpemlin. 使用 cenpemlin 探测中心粒-着丝点核心复合体 CENP-L/M 的组装。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-09-06 DOI: 10.1093/jmcb/mjae035

Olanrewaju Ayodeji Durojaye, Fengrui Yang, Xinjiao Gao, Felix Aikhionbare, Liangyu Zhang, Xing Liu, Xuebiao Yao

引用次数: 0

Correction to: Mitochondrial aldehyde dehydrogenase rescues against diabetic cardiomyopathy through GSK3β-mediated preservation of mitochondrial integrity and Parkin-mediated mitophagy. 更正为线粒体醛脱氢酶通过 GSK3β 介导的线粒体完整性保护和 Parkin 介导的有丝分裂，拯救糖尿病心肌病。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-09-04 DOI: 10.1093/jmcb/mjae032

引用次数: 0

PHLDA2 is critical for p53-mediated ferroptosis and tumor suppression. PHLDA2 对 p53 介导的铁变态反应和肿瘤抑制至关重要。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-29 DOI: 10.1093/jmcb/mjae033

Xin Yang, Wei Gu

引用次数: 0

Comments on 'Adeno-to-squamous transition drives resistance to KRAS inhibition in LKB1 mutant lung cancer'. 关于 "LKB1突变型肺癌中腺癌向鳞癌转化驱动对KRAS抑制剂的耐药性 "的评论

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-26 DOI: 10.1093/jmcb/mjae013

Xinyuan Tong, Ningxia Zhang, Yun Xue, Hongbin Ji

引用次数: 0

Regulation of m6Am RNA modification and its implications in human diseases. m6Am RNA修饰的调控及其对人类疾病的影响。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-26 DOI: 10.1093/jmcb/mjae012

Hao Jin, Zhouyuanjing Shi, Tianhua Zhou, Shanshan Xie

{"title":"Regulation of m6Am RNA modification and its implications in human diseases.","authors":"Hao Jin, Zhouyuanjing Shi, Tianhua Zhou, Shanshan Xie","doi":"10.1093/jmcb/mjae012","DOIUrl":"10.1093/jmcb/mjae012","url":null,"abstract":"N 6,2'-O-dimethyladenosine (m6Am) is a prevalent modification frequently found at the 5' cap-adjacent adenosine of messenger RNAs (mRNAs) and small nuclear RNAs (snRNAs) and the internal adenosine of snRNAs. This dynamic and reversible modification is under the regulation of methyltransferases phosphorylated CTD interacting factor 1 and methyltransferase-like protein 4, along with the demethylase fat mass and obesity-associated protein. m6Am RNA modification plays a crucial role in the regulation of pre-mRNA splicing, mRNA stability, and translation, thereby influencing gene expression. In recent years, there has been growing interest in exploring the functions of m6Am and its relevance to human diseases. In this review, we provide a comprehensive overview of the current knowledge concerning m6Am, with a focus on m6Am-modifying enzymes, sequencing approaches for its detection, and its impacts on pre-mRNA splicing, mRNA stability, and translation regulation. Furthermore, we highlight the roles of m6Am in the context of obesity, viral infections, and cancers, unravelling its underlying regulatory mechanisms.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11345611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A polarized multicomponent foundation upholds ciliary central microtubules. 纤毛中心微管由极化的多成分基础支撑。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-20 DOI: 10.1093/jmcb/mjae031

Qingxia Chen, Huijie Zhao, Xinwen Pan, Chuyu Fang, Benhua Qiu, Jingting Guo, Xiumin Yan, Xueliang Zhu

{"title":"A polarized multicomponent foundation upholds ciliary central microtubules.","authors":"Qingxia Chen, Huijie Zhao, Xinwen Pan, Chuyu Fang, Benhua Qiu, Jingting Guo, Xiumin Yan, Xueliang Zhu","doi":"10.1093/jmcb/mjae031","DOIUrl":"https://doi.org/10.1093/jmcb/mjae031","url":null,"abstract":"Cilia's back-and-forth beat pattern requires a central pair (CP) of microtubules. However, the mechanism by which the CP is upheld above the transition zone (TZ) remains unclear. Here, we showed that a rod-like substructure marked by Cep131 and ciliary Centrin serves as a polarized CP-supporting foundation. This CP-foundation (CPF) was assembled independently of the CP during ciliogenesis in mouse ependymal cells. It protruded from the distal end of the basal body out of the TZ to enwrap the proximal end of the CP. Through proximity labeling, we identified 26 potential CPF components, among which Ccdc148 specifically localized at the proximal region of Centrin-decorated CPF and was complementary to the Cep131-enriched distal region. Cep131 deficiency abolished the CPF, resulting in CP penetration into the TZ. Consequently, cilia became prone to ultrastructural abnormality and paralysis, and Cep131-deficient mice were susceptible to late-onset hydrocephalus. In addition to Centrin, phylogenetic analysis also indicated conservations of Ccdc131 and Ccdc148 from protists to mammals, suggesting that the CPF is an evolutionarily conserved multicomponent CP-supporting platform in cilia.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142010230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling gastric intestinal metaplasia in 3D organoids using nitrosoguanidine. 利用亚硝基胍在三维有机体中模拟胃肠化生。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-17 DOI: 10.1093/jmcb/mjae030

Yuan Li, Jiena Chen, Tao Li, Jie Lin, Haocheng Zheng, Nadia Johnson, Xuebiao Yao, Xia Ding

{"title":"Modeling gastric intestinal metaplasia in 3D organoids using nitrosoguanidine.","authors":"Yuan Li, Jiena Chen, Tao Li, Jie Lin, Haocheng Zheng, Nadia Johnson, Xuebiao Yao, Xia Ding","doi":"10.1093/jmcb/mjae030","DOIUrl":"https://doi.org/10.1093/jmcb/mjae030","url":null,"abstract":"Gastric intestinal metaplasia (GIM) represents a precancerous stage characterized by morphological and pathophysiological changes in the gastric mucosa, where gastric epithelial cells transform into a phenotype resembling that of intestinal cells. Previous studies have demonstrated that the intragastric administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induces both gastric carcinoma and intestinal metaplasia in mice. Here, we show that MNNG induces GIM in three-dimensional (3D) mouse organoids. Our histological analyses reveal that MNNG-induced gastric organoids undergo classical morphological alterations, exhibiting a distinct up-regulation of CDX2 and MUC2, along with a down-regulation of ATP4B and MUC6. Importantly, metaplastic cells observed in MNNG-treated organoids originate from MIST1+ cells, indicating their gastric chief cell lineage. Functional analyses show that activation of the RAS signaling pathway drives MNNG-induced metaplasia in 3D organoids, mirroring the characteristics observed in human GIM. Consequently, modeling intestinal metaplasia using 3D organoids offers valuable insights into the molecular mechanisms and spatiotemporal dynamics of the gastric epithelial lineage during the development of intestinal metaplasia within the gastric mucosa. We conclude that the MNNG-induced metaplasia model utilizing 3D organoids provides a robust platform for developing preventive and therapeutic strategies to mitigate the risk of gastric cancer before precancerous lesions occur.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterile activation of RNA-sensing pathways in autoimmunity. 无菌激活自身免疫中的 RNA 传感途径

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-08-14 DOI: 10.1093/jmcb/mjae029

Jiaxin Li, Junyan Zhu, Hui Yang, Fajian Hou

引用次数: 0

Dual role of PpV in Drosophila crystal cell proliferation and survival. PpV 在果蝇晶体细胞增殖和存活中的双重作用

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-07-31 DOI: 10.1093/jmcb/mjae028

Wang Luo, Fang Zhang, Fangzhen Zhao, Yang Fang, Long Zhao, Ying Su

{"title":"Dual role of PpV in Drosophila crystal cell proliferation and survival.","authors":"Wang Luo, Fang Zhang, Fangzhen Zhao, Yang Fang, Long Zhao, Ying Su","doi":"10.1093/jmcb/mjae028","DOIUrl":"https://doi.org/10.1093/jmcb/mjae028","url":null,"abstract":"Drosophila melanogaster crystal cells are a specialized type of blood cells for innate immune process upon injury. Under normal conditions, crystal cells rarely proliferate and constitute a small proportion of fly blood cells. Notch signaling has been known to guide the cell fate determination of crystal cells and maintain their survival. Here, we reported that protein phosphatase V (PpV), the unique catalytic subunit of protein phosphatase 6 in Drosophila, is a novel regulator of crystal cell proliferation and integrity. We found that PpV proteins highly accumulated in crystal cells in the larval hematopoietic organ termed the lymph gland. Silencing PpV using RNA interference led to increased crystal cell proliferation in a Notch-independent manner and induced crystal cell rupture dependent on Notch signaling. Moreover, additive PpV prevented the rupture of crystal cells in lymph glands upon a needle injury, suggesting the involvement of PpV in wound healing. Altogether, our results indicated that PpV plays a dual role in lymph glands, preventing crystal cell proliferation to limit the cell number, as well as inhibiting crystal cell rupture to maintain their survival.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral factors and their counteraction by HIV-1: many uncovered and more to be discovered. 抗病毒因子及其对 HIV-1 的反作用：发现了许多，还有更多有待发现。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2024-07-29 DOI: 10.1093/jmcb/mjae005

Dorota Kmiec, Frank Kirchhoff

引用次数: 0