Journal of Molecular Cell Biology最新文献_第2页

Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts. syndecan-4在损伤皮肤中抑制EphA2可调节成纤维细胞的聚集。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-28 DOI: 10.1093/jmcb/mjae054

Rebecca Brooks, Xianhui Wei, Mang Leng Lei, Francisca Cisterna Cid, James A Roper, Rosalind C Williamson, Mark D Bass

{"title":"Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts.","authors":"Rebecca Brooks, Xianhui Wei, Mang Leng Lei, Francisca Cisterna Cid, James A Roper, Rosalind C Williamson, Mark D Bass","doi":"10.1093/jmcb/mjae054","DOIUrl":"10.1093/jmcb/mjae054","url":null,"abstract":"Upon injury, fibroblasts in the surrounding tissue become activated, migrating into the wound in a controlled manner. Once they arrive, they contract the wound and remodel the stroma. While certain cell surface receptors promote fibroblast migration, others cause repulsion between fibroblasts upon contact, seemingly opposing their clustering within the wound bed. Eph receptor-ephrin interactions on colliding cells trigger this repulsion, but how fibroblasts transition to clustering behaviour during healing remains unclear. Syndecan-4 modulates transmembrane receptors involved in wound healing, including receptors for the extracellular matrix and growth factors. As a result, Sdc4-/- mice experience delayed healing due to impaired fibroblast recruitment. In this study, we report that syndecan-4 also regulates fibroblast repulsion during wound healing. We discover that syndecan-4 inhibits the expression and signalling of EphA2 by activating PKCα. Changes in syndecan-4 expression, such as those observed during wound healing, alter fibroblast behaviour from repulsion to adhesion upon cell collision by modulating EphA2 levels. Moreover, we find that EphA2 expression is suppressed in wound bed fibroblasts in a syndecan-4-dependent manner, explaining how fibroblast clustering is achieved during wound healing.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12205308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A long noncoding RNA with enhancer-like function in pig zygotic genome activation. 猪受精卵基因组激活中一种具有增强子样功能的长链非编码RNA。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-28 DOI: 10.1093/jmcb/mjae061

Renyue Wei, Yanbin Yue, Yinhuan Wu, Chenyuan Zhang, Jun-Xue Jin, Zhonghua Liu, Jiaqiang Wang

{"title":"A long noncoding RNA with enhancer-like function in pig zygotic genome activation.","authors":"Renyue Wei, Yanbin Yue, Yinhuan Wu, Chenyuan Zhang, Jun-Xue Jin, Zhonghua Liu, Jiaqiang Wang","doi":"10.1093/jmcb/mjae061","DOIUrl":"10.1093/jmcb/mjae061","url":null,"abstract":"The zygotic genome activation (ZGA) is crucial for the development of pre-implantation embryos. Long noncoding RNAs (lncRNAs) play significant roles in many biological processes, but the study on their role in the early embryonic development of pigs is limited. In this study, we identify lncFKBPL as an enhancer-type lncRNA essential for pig embryo development. lncFKBPL is expressed from the 4-cell stage to the morula stage in pig embryos, and interference with lncFKBPL leads to a developmental arrest at the 8-cell stage. Mechanistic investigations uncover that lncFKBPL is able to bind to MED8, thereby mediating enhancer activity and regulating FKBPL expression. Additionally, FKBPL interacts with the molecular chaperone protein HSP90AA1, stabilizing CDK9 and boosting its protein-level expression. Elevated CDK9 levels enhance Pol II phosphorylation, facilitating ZGA. Our findings illuminate the role of lncFKBPL as an enhancer lncRNA in pig ZGA regulation and early embryo development, providing a foundation for further exploration in this area.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivalent interactions of Septin 6 promote the establishment of epithelial cell polarity. Septin 6的多价相互作用促进上皮细胞极性的建立。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-28 DOI: 10.1093/jmcb/mjaf003

Weihong Fu, Xueying Wang, Mussarat Rafiq, Hengyi Shao, Cunyu Wang, Dongmei Wang, Changlu Tao, Chuanhai Fu, Barbara Zieger, Xing Liu, Xuebiao Yao, Liangyu Zhang

{"title":"Multivalent interactions of Septin 6 promote the establishment of epithelial cell polarity.","authors":"Weihong Fu, Xueying Wang, Mussarat Rafiq, Hengyi Shao, Cunyu Wang, Dongmei Wang, Changlu Tao, Chuanhai Fu, Barbara Zieger, Xing Liu, Xuebiao Yao, Liangyu Zhang","doi":"10.1093/jmcb/mjaf003","DOIUrl":"10.1093/jmcb/mjaf003","url":null,"abstract":"Septins, components of the fourth cytoskeleton, play an indispensable role in establishing and maintaining epithelial cell polarity. However, the molecular mechanisms underlying the dynamic assembly of higher-order septin structures and the establishment of epithelial cell polarity remain elusive. Here, we show that septins form a previously unrecognized dynamic structure with liquid-like properties in polarized Madin-Darby canine kidney cells. We identified Septin 6 (SEPT6) as the key human septin that undergoes liquid-liquid phase separation (LLPS) both in vitro and in vivo through weak, multivalent interactions mediated by its C-terminal tail. SEPT6 mutants defective in LLPS in vitro also fail to support adherens junction integrity and cell polarity establishment in 2D and 3D cell cultures. Our findings indicate that weak, multivalent interactions are essential for the assembly of higher-order septin structures in cells. We propose that these interactions, in conjunction with conventional interactions between folded domains, generate partially ordered septin assemblies that support the apical-basal axis and lumen formation in metazoans.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A look back at the Virology Departmental Days of the Institut Pasteur (Le Touquet, May 13-15, 2024). 巴斯德研究所病毒学系日回顾（2024 年 5 月 13-15 日，勒图凯）。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-12 DOI: 10.1093/jmcb/mjae052

Rubén González, Cassandra Koh, Bérangère Virlon, Sarah Hélène Merkling, Jean-Pierre Vartanian

引用次数: 0

Application of nanomedicines in tumor immunotherapy. 纳米药物在肿瘤免疫治疗中的应用。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-12 DOI: 10.1093/jmcb/mjae055

Zirui Gao, Dandan Wan, Min Luo, Xiawei Wei

{"title":"Application of nanomedicines in tumor immunotherapy.","authors":"Zirui Gao, Dandan Wan, Min Luo, Xiawei Wei","doi":"10.1093/jmcb/mjae055","DOIUrl":"10.1093/jmcb/mjae055","url":null,"abstract":"Tumor immunotherapy has emerged as a formidable strategy, demonstrating substantial achievements in the field of cancer treatment. Despite its remarkable success, intrinsic limitations such as insufficient targeting capabilities, side effects, and resistance to immunotherapy hinder its efficacy. To address these challenges, the utilization of nanomedicines in tumor immunotherapy has been broadly explored, capitalizing on their advantages of targeting delivery capability, loading capacity, modifiability, and biocompatibility. Through rational design approaches, nanomedicines are engineered to meet diverse delivery requirements and synergize with different regimens to maximize therapeutic efficacy while alleviating side effects. This review initially discusses the challenges associated with tumor immunotherapy and underscores the pivotal role played by nanomedicines in overcoming these obstacles. Subsequently, representative types of nanoparticles are systematically introduced based on their structural properties, advantages, potential limitations, and future research directions. Special emphasis is placed on recent advancements in a range of nanomedicines designed for specific tumor immunotherapy strategies. Finally, the clinical applications as well as prospects of nanomedicines are discussed.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule plus-end tracking protein EB1 orchestrates mitotic progression via liquid-liquid phase separation. 微管+末端跟踪蛋白EB1通过液-液相分离调控有丝分裂进程。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-12 DOI: 10.1093/jmcb/mjae059

Mengjie Li, Ming Dai, Xing Hong, Yunze Li, Xiyu Wang, Yini Lin, Tahir Ullah, Yuxin Zhu, Kai Jiang, Zhikai Wang, Zhonghuai Hou, Kai Zhang, Xuebiao Yao, Xiaoyu Song, Xing Liu

引用次数: 0

Early differentiation of committed erythroid cells defined by miR-144/451 expression. 由miR-144/451表达决定的红细胞早期分化。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-12 DOI: 10.1093/jmcb/mjae057

Xiaohong Li, Yong Dong, Xu Pan, Wencui Sun, Yuan Xue, Ya Zhou, Mowen Lai, Yonggang Zhang, Feng Ma

{"title":"Early differentiation of committed erythroid cells defined by miR-144/451 expression.","authors":"Xiaohong Li, Yong Dong, Xu Pan, Wencui Sun, Yuan Xue, Ya Zhou, Mowen Lai, Yonggang Zhang, Feng Ma","doi":"10.1093/jmcb/mjae057","DOIUrl":"10.1093/jmcb/mjae057","url":null,"abstract":"Before committing to an erythroid cell lineage, hematopoietic stem cells differentiate along a myeloid cell pathway to generate megakaryocyte-erythroid biopotential progenitor cells in bone marrow. Recent studies suggest that erythroid progenitors (EryPs) could be generated at the level of common myeloid progenitors (CMPs). However, due to a lack of suitable markers, little is known about the early differentiation of these committed EryP cells during CMP development. Herein, using miR-144/451-eGFP knock-in mice, we found that early differentiation of committed erythroid cells could be defined by miR-144/451 expression within CMPs. Single-cell RNA sequencing showed that miR-144/451+ progenitors show obvious differentiation characteristics of erythroid lineage cells and diverge from megakaryocyte and other myeloid cell lineages. These progenitors exclusively give rise to erythroid cells, both in vitro and in vivo, and the commitment to an erythroid cell lineage is accompanied by loss of CD53 expression. Our findings will facilitate further understanding of the molecular mechanisms governing erythroid development and support the identification of therapeutic targets for diseases related to erythrocyte development.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPI transamidase complex is required for primordial germ cell migration and development in zebrafish. GPI转氨酶复合体是斑马鱼原始生殖细胞迁移和发育所必需的。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-06-12 DOI: 10.1093/jmcb/mjae058

Weiying Zhang, Yaqi Li, Jing Chen, Likun Yao, Bingjie Zhang, Lin Zhang, Boqi Liu, Weimin Shen, Anming Meng, Xiaotong Wu

{"title":"GPI transamidase complex is required for primordial germ cell migration and development in zebrafish.","authors":"Weiying Zhang, Yaqi Li, Jing Chen, Likun Yao, Bingjie Zhang, Lin Zhang, Boqi Liu, Weimin Shen, Anming Meng, Xiaotong Wu","doi":"10.1093/jmcb/mjae058","DOIUrl":"10.1093/jmcb/mjae058","url":null,"abstract":"Proteins without transmembrane domains could be anchored to the cell surface for regulating various biological processes when covalently linked to glycosylphosphatidylinositol (GPI) molecules by the GPI transamidase (GPIT) complex. However, it remains poorly understood whether and how the GPIT complex affects primordial germ cell (PGC) development. In this study, we report the important roles of the GPIT complex in PGC migration and development in zebrafish embryos. Mutation of pigu or pigk, both encoding essential GPIT complex subunits, resulted in defective PGC migration with ectopically located PGCs and reduction of PGC counts. Notably, a detailed analysis of filopodia in PGCs revealed the attenuated polarity of filopodia distribution along the migration direction in mutant embryos. PGC transplantation and PGC-specific rescue experiments demonstrated that both PGC and somatic cell-expressed Pigu are required for PGC migration. Furthermore, expression levels of PGC-specific genes decreased in pigu mutant PGCs with the derepression of somatic cell genes. Hence, we propose that the GPIT complex plays a critical role during PGC migration and development.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased serum β-hydroxybutyrate/acetoacetate ratio and aggravated histological liver inflammation in females with metabolic dysfunction-associated steatotic liver disease and polycystic ovary syndrome. 代谢功能障碍相关性脂肪性肝病和多囊卵巢综合征女性血清β-羟丁酸/乙酰乙酸比值升高，肝脏组织学炎症加重。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-05-22 DOI: 10.1093/jmcb/mjae048

Xiaopeng Zhu, Guligeina Aikebaier, Xilei Ban, Qingxia Huang, Hongmei Yan, Xinxia Chang, Xinyu Yang, Xiaoyang Sun, Huiru Tang, Hua Bian, Xin Gao, Mingfeng Xia

引用次数: 0

Low-dose quinine targets KCNH6 to potentiate glucose-induced insulin secretion. 低剂量奎宁靶向KCNH6，增强葡萄糖诱导的胰岛素分泌。

IF 5.3 2区生物学

Journal of Molecular Cell Biology Pub Date : 2025-05-22 DOI: 10.1093/jmcb/mjae051

Feng-Ran Xiong, Juan-Juan Zhu, Xiao-Rong Zhu, Jing Lu, Jin-Kui Yang

{"title":"Low-dose quinine targets KCNH6 to potentiate glucose-induced insulin secretion.","authors":"Feng-Ran Xiong, Juan-Juan Zhu, Xiao-Rong Zhu, Jing Lu, Jin-Kui Yang","doi":"10.1093/jmcb/mjae051","DOIUrl":"10.1093/jmcb/mjae051","url":null,"abstract":"Insulin secretion is mainly regulated by two electrophysiological events, depolarization initiated by the closure of adenosine triphosphate (ATP)-sensitive K+ (KATP) channels and repolarization mediated by K+ efflux. Quinine, a natural component commonly used for the treatment of malaria, has been reported to directly stimulate insulin release and lead to hypoglycemia in patients during treatment through inhibiting KATP channels. In this study, we verified the insulinotropic effect of quinine on the isolated mouse pancreatic islets. We also revealed that low-dose quinine (<20 μM) did not directly provoke Ca2+ spikes or insulin secretion under low-glucose conditions but potentiated Ca2+ influx and insulin secretion induced by high glucose, which cannot be explained by KATP inhibition. KCNH6 (hERG2) is a voltage-dependent K+ (Kv) channel that plays a critical role in the repolarization of pancreatic β cells. Patch clamp experiments showed that quinine inhibited hERG channels at low micromolar concentrations. However, whether quinine can target KCNH6 to potentiate glucose-induced insulin secretion remains unclear. Here, we showed that in vivo administration of low-dose quinine (25 mg/kg) improved glucose tolerance and increased glucose-induced insulin release in wild-type control mice but not in Kcnh6-β-cell-specific knockout (βKO) mice. Consistently, in vitro treatment of primary islet β cells with low-dose quinine (10 μM) prolonged action potential duration and augmented glucose-induced Ca2+ influx in the wild-type control group but not in the Kcnh6-βKO group. Our results demonstrate that KCNH6 plays an important role in low-dose quinine-potentiated insulin secretion and provide new insights into KCNH6-targeted drug development.","PeriodicalId":16433,"journal":{"name":"Journal of Molecular Cell Biology","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0