Journal of Neuroscience Methods最新文献

{"title":"Development of high-content screening assay for gene silencing in adult sensory neurons","authors":"Phillip Canete ,&nbsp;David Do ,&nbsp;Richard Lie ,&nbsp;Grace Woodruff ,&nbsp;Rodrigo Lopez Gonzalez ,&nbsp;Gunnar Poplawski","doi":"10.1016/j.jneumeth.2025.110500","DOIUrl":"10.1016/j.jneumeth.2025.110500","url":null,"abstract":"<div><h3>Background</h3><div>High-content screening in post-mitotic neurons faces challenges due to low transfection efficiency and expensive viral or electroporation methods. To accelerate discovery specifically in <em><strong>adult</strong></em> sensory neurons, we sought a scalable, low-cost platform that preserves neuronal health.</div></div><div><h3>Method</h3><div>We developed a 384-well lipid-based siRNA screening assay using adult EGFP-expressing Fischer 344 DRG neurons. Key optimizations included a systematic comparison of plate plastics and lipid/siRNA ratios and reagents, yielding maximal knock-down with minimal toxicity.</div></div><div><h3>Results</h3><div>Optimal conditions (0.12 μL reagent, 2.5 pmol siRNA/well) reduced EGFP fluorescence by ≥ 50 % in 45 % of neurons, with mean knockdown efficiencies up to 60 % and minimal impact on neurite length. PTEN-targeting siRNAs increased neurite outgrowth by 40 % (p &lt; 0.001), while death siRNA reduced length by 30 % (p &lt; 0.001), demonstrating sensitivity to both stimulatory and inhibitory gene perturbations.</div></div><div><h3>Comparison</h3><div>Our approach offers substantially lower cost and higher throughput than alternatives. Relative to electroporation protocols for adult DRG neurons, reagent cost is reduced ∼12-fold and hands-on time drops from ∼2 days to ∼3 h, while eliminating specialized equipment. Notably, the assay was optimized for cost-efficiency and scalability; for instance, our 384-well format protocol can screen hundreds of genes in triplicate for under $10,000, making high-content screening feasible in smaller laboratories.</div></div><div><h3>Conclusions</h3><div>This platform enables rapid, cost-effective evaluation of hundreds to thousands of candidate genes in adult sensory neurons, facilitating identification of neurite growth regulators.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110500"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conducting interslice stimulation for concurrent TMS-fMRI.","authors":"J B Jackson, C L Scrivener, M M Correia, M Mada, A Woolgar","doi":"10.1016/j.jneumeth.2025.110513","DOIUrl":"https://doi.org/10.1016/j.jneumeth.2025.110513","url":null,"abstract":"<p><strong>Background: </strong>Transcranial magnetic stimulation (TMS) concurrent with functional magnetic resonance imaging (fMRI) can provide insights into the causal relationships between brain activity and behaviour. However, TMS pulses can cause artifacts in fMRI data, but these can be avoided if they are presented in short gaps between MRI slice acquisitions (interslice TMS-fMRI).</p><p><strong>New method: </strong>We collected TMS-fMRI data to provide 1) guidance on the gap required and 2) a higher-level framework and code for researchers to test their own protocols. We quantified signal dropout and temporal signal-to-noise ratio in fMRI data (spherical phantom) for TMS pulses presented from up to 100ms before and after slice excitation. We delivered up to 3 pulses per volume with interslice gaps of 37.5ms/100ms (slice time 62.5ms), two 7-channel TMS-dedicated surface coils, and a multiband sequence (factor=2), on a Siemens 3T Prisma^fit scanner. We repeated a subset of parameters with a human participant.</p><p><strong>Results: </strong>We observed minimal data contamination when pulses were applied at least -20ms/+50ms from slice excitation, and confirmed this approach can be used with 10Hz TMS.</p><p><strong>Comparison with existing methods: </strong>Compared to other strategies that avoid TMS pulse-related artifacts, interslice allows for greater flexibility in terms of timing of the TMS pulse, MRI read out and any stimulus presentation.</p><p><strong>Conclusion: </strong>A stimulation frequency faster than 10Hz would require a shorter gap or shorter slice acquisition times. Further, stimulator intensity, slice orientation, and the number of TMS pulses affected data quality and are important considerations for researchers when setting up their own protocol.</p>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110513"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to \"Lightweight attention mechanisms for EEG emotion recognition for brain computer interface\".","authors":"Naresh Kumar Gunda, Mohammed I Khalaf, Shaleen Bhatnagar, Aadam Quraishi, Leeladhar Gudala, Ashok Kumar Pamidi Venkata, Faisal Yousef Alghayadh, Shtwai Alsubai, Vaibhav Bhatnagar","doi":"10.1016/j.jneumeth.2025.110502","DOIUrl":"10.1016/j.jneumeth.2025.110502","url":null,"abstract":"","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110502"},"PeriodicalIF":2.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling lamina specific pyramidal neurons using postmortem human formalin fixed paraffin embedded frontal cortex tissue in combination with digital spatial profiling","authors":"Aleksandra Stanisavljevic ,&nbsp;Kyrillos W. Ibrahim ,&nbsp;Philip H. Stavrides ,&nbsp;Christopher Bare ,&nbsp;Melissa J. Alldred ,&nbsp;Adriana Heguy ,&nbsp;Ralph A. Nixon ,&nbsp;Stephen D. Ginsberg","doi":"10.1016/j.jneumeth.2025.110512","DOIUrl":"10.1016/j.jneumeth.2025.110512","url":null,"abstract":"<div><h3>Background</h3><div>Digital spatial profiling (DSP) is an innovative approach to perform RNA sequencing (RNA-seq), including in neuronal populations. DSP enables expression profiling linking RNA-seq data to spatially characterized samples utilizing tissue bound probes. We employ the GeoMx DSP system for spatial characterization of transcriptomic data from lamina specific pyramidal neurons and cortical ribbons containing admixed cell types using human postmortem brain tissue.</div><div><strong>New method</strong></div><div>We established a protocol using human postmortem formalin fixed paraffin embedded (FFPE) frontal cortex tissue from nondemented human control brains. Layer III (L3) and Layer V (L5) pyramidal neurons from Brodmann area 9 were identified with the neuronal marker Ca^2 + /calmodulin-dependent protein kinase II and selected for probe collection.</div></div><div><h3>Results</h3><div>This approach significantly reduced the amount of FFPE tissue needed for robust single population RNA-seq. We demonstrate ∼20 identified L3 or L5 pyramidal neurons or one lamina-specific cortical ribbon from a single 5 µm thick section is sufficient to generate robust RNA-seq reads. Bioinformatic analysis of neurons and ribbons showed notable similarities and differences reflective of the single neuron and multiple admixed cell types within the former and latter, respectively.</div><div><strong>Comparison with existing methods</strong></div><div>Protocols exist for DSP of postmortem human FFPE brain tissue. However, this new approach enables profiling small groups of ∼14–21 pyramidal neurons using the GeoMx DSP platform.</div></div><div><h3>Conclusions</h3><div>This optimized DSP assay provides high resolution RNA-seq data demonstrating utility and versatility of the GeoMx platform for individually characterized neurons and isolated cortical ribbons within postmortem FFPE human brain tissue for downstream analyses.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110512"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Under-oil neuronal cell culture: Enhanced system stability, yield, and modulated oxygen environment","authors":"Nai-Wen Liang ,&nbsp;Ligi Milesh ,&nbsp;Karina N. Buttram ,&nbsp;Jessica L. Park ,&nbsp;Jing Zhang ,&nbsp;Luke A. Summey ,&nbsp;Christian Franck ,&nbsp;David J. Beebe ,&nbsp;Hau D. Le ,&nbsp;Chao Li","doi":"10.1016/j.jneumeth.2025.110511","DOIUrl":"10.1016/j.jneumeth.2025.110511","url":null,"abstract":"<div><h3>Background</h3><div>Culturing neuronal cells <em>in vitro</em>, especially at smaller scales with reduced media volumes, has been challenging due to the limited proliferation of mature neurons and the inherent high sensitivity of neuronal cells to environmental fluctuations.</div></div><div><h3>New method</h3><div>In this study, we report a neuronal cell culture method that leverages oil overlay and an autonomously regulated oxygen microenvironment (AROM), in which primary rat cortical cells and human neural progenitor cells (NPCs) were cultured in standard well plates with an oil overlay on top of the media layer. The oil overlay prevents evaporation and achieves <em>in vivo</em>-like oxygen concentrations without the use of glove boxes or hypoxic chambers.</div></div><div><h3>Results</h3><div>This oil overlay method achieved &gt; 95 % yield of viable replicates after up to 30 days. Human NPCs cultured under the oil overlay for 15 days exhibited sustained viability without requiring media change. Additionally, oil overlays create a modulated oxygen microenvironment (i.e., AROM) that mimics <em>in vivo</em> conditions, capable of maintaining and restoring optimal oxygen concentrations after disturbances.</div></div><div><h3>Comparison with existing method</h3><div>In contrast, existing method (no-oil controls) resulted in &lt; 20 % yield, low viability for human NPCs (11 % versus 89 % with oil overlay), and oxygen concentrations that returns to ambient levels (21 % oxygen).</div></div><div><h3>Conclusion</h3><div>Overall, these results support the oil overlay method as a robust small-scale neuronal cell culture system, offering improved stability and higher yield. The results also underscore the critical role of the oxygen microenvironment in supporting neuronal cell viability, maintenance, and growth.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110511"},"PeriodicalIF":2.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of transcranial electrical stimulation on intracranial and cerebral perfusion pressures in patients with severe craniocerebral injury: A novel methodological approach","authors":"Ailiang Ge,&nbsp;Jinan Bai,&nbsp;Yan Wang,&nbsp;Xin Li","doi":"10.1016/j.jneumeth.2025.110498","DOIUrl":"10.1016/j.jneumeth.2025.110498","url":null,"abstract":"<div><h3>Background</h3><div>Transcranial electrical stimulation (tES) protects brain cells and blood flow, making it an intriguing treatment option for skull and brain injury victims. Understanding how transcranial electroencephalography (tES) affects cerebral perfusion pressure (CPP) and intracranial pressure (ICP) might improve patient outcomes. Most techniques for evaluating ICP and CPP include intrusive procedures that might cause infections and hiccups. Additionally, current methods cannot optimize patient results by changing stimulation settings in real-time.</div></div><div><h3>New Method</h3><div>The Optimizing tES Parameters for Neuroprotection (O-tES-PN) approach addresses these issues. The system has many monitoring tools and an adaptive tES control mechanism. The O-tES-PN continually adjusts its dynamic stimulation settings based on intracranial and cerebral blood pressure. This keeps cerebral blood flow constant and reduces damage.</div></div><div><h3>Results</h3><div>Our findings show that stable ICP and CPP enhance CBF modulation with the O-tES-PN design. The device may modify settings in real-time to give more concentrated and effective treatments with fewer adverse effects.</div></div><div><h3>Comparison with Existing Methods</h3><div>Compared to invasive therapies, O-tES-PN is safer. It also improves therapy parameter control. Current technology allows continuous monitoring and stimulation level changes, which are limitations of previous systems.</div></div><div><h3>Conclusions</h3><div>The O-tES-PN treatment is less risky than other invasive treatments. Controlling the parameters of the treatment is also improved. The limitations of earlier systems are overcome by the current technology, which enables continuous monitoring and adjustments in stimulation level.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110498"},"PeriodicalIF":2.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using DeepLabCut-Live to probe state dependent neural circuits of behavior with closed-loop optogenetic stimulation","authors":"Melissa Gonzalez ,&nbsp;Mark A. Gradwell ,&nbsp;Joshua K. Thackray ,&nbsp;Kanaksha K. Temkar ,&nbsp;Komal R. Patel ,&nbsp;Victoria E. Abraira","doi":"10.1016/j.jneumeth.2025.110495","DOIUrl":"10.1016/j.jneumeth.2025.110495","url":null,"abstract":"<div><h3>Background</h3><div>Closed-loop behavior paradigms allow for real-time investigation of state-dependent neural circuits underlying behavior. However, studying context-dependent locomotor perturbations is challenging due to limitations in molecular tools and techniques for real-time manipulation of spinal circuits.</div></div><div><h3>New method</h3><div>We developed a novel closed-loop optogenetic stimulation paradigm that leverages DeepLabCut-Live pose estimation to manipulate primary sensory afferent activity at specific phases of the locomotor cycle in mice. A compact DeepLabCut model was trained to track hindlimb kinematics in real-time and integrated into the Bonsai visual programming framework. This system enabled LED triggered photo-stimulation of sensory neurons expressing channelrhodopsin based on user-defined pose-based criteria, such as stance or swing phase.</div></div><div><h3>Results</h3><div>Optogenetic activation of nociceptive TRPV1⁺ sensory neurons during treadmill locomotion reliably evoked paw withdrawal responses. Stimulation during the stance phase generated a brief withdrawal and impacted the duration of the following swing phase. Stimulation during the swing phase increased the height of paw withdrawal during swing and reduced the duration of the following stance phase.</div></div><div><h3>Comparison with existing methods</h3><div>This method allows for high spatiotemporal precision in manipulating spinal circuits based on locomotor phase. Unlike previous approaches, this closed-loop system accounts for state-dependent nature of sensorimotor responses, enabling controlled, real-time modulation of locomotion.</div></div><div><h3>Conclusions</h3><div>Integrating DeepLabCut-Live with optogenetics provides a powerful tool for dissecting the context-dependent role of sensory feedback and spinal interneurons in locomotion. This technique opens new avenues for uncovering the neural substrates of state-dependent behaviors and has broad applicability for studies of real-time closed-loop manipulation based on pose estimation.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"422 ","pages":"Article 110495"},"PeriodicalIF":2.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of somatosensory evoked potentials through fine intrafascicular stimulation via flexible carbon nanotube yarn electrodes under different isoflurane anaesthetics","authors":"Yapeng Zhang ,&nbsp;Shang Deng ,&nbsp;Muqiang Jian ,&nbsp;Xiaohua Zhang ,&nbsp;Yao Chen ,&nbsp;Jiguang Wang ,&nbsp;Xiaohong Sui","doi":"10.1016/j.jneumeth.2025.110496","DOIUrl":"10.1016/j.jneumeth.2025.110496","url":null,"abstract":"<div><h3>Background</h3><div>While most current studies have focused on the effects of transcutaneous and extrafascicular stimulation on SEPs, intrafascicular stimulation offers a more advantageous alternative. By providing closer access to target afferent fibers, it allows for lower stimulation currents and greater nerve fiber selectivity, making it a promising approach for achieving fine tactile feedback.</div></div><div><h3>New method</h3><div>This study employed intrafascicular stimulation with carbon nanotube yarn (CNTy) electrodes to explore its impact on tactile feedback under different levels of isoflurane anaesthesia in rats. Bipolar and monopolar stimulation were applied to stimulate tibial nerves and the SEPs were recorded to analyse quantitatively. <em>Results:</em> The stimulation current threshold increased with increasing anaesthetic concentration for both bipolar and monopolar stimulation for each rat. As the anaesthesia concentration increased, the amplitude progressively decreased (P &lt; 0.001, 1.0 % vs 2.0 %, monopolar; P &lt; 0.01, 1.0 % vs 2.0 %, bipolar), the latency was prolonged (P &lt; 0.01, 1.0 % vs 2.0 %, monopolar; P &lt; 0.05, 1.0 % vs 2.0 %, bipolar), and the spectral power of early and late components significantly decreased (P &lt; 0.01, 1.0 % vs 2.0 %, monopolar, P_early; P &lt; 0.05, 1.0 % vs 2.0 %, monopolar, P_late). <em>Comparison with existing methods:</em> For the first time, this study utilized CNTy electrodes via intrafascicular stimulation to investigate the characteristics of SEPs under varying isoflurane concentrations. <em>Conclusions:</em> This study provides a solid foundation for quantifying tactile feedback of intrafascicular stimulation during rat experiments, and reveals among the three anesthetic concentrations tested, 1.0 % exerted the minimal impact on all measured SEP characteristics in rats.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110496"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability and suitability of rat pup organotypic spinal cord slice cultures in a transection injury model","authors":"Ciara Shortiss ,&nbsp;Linda Howard ,&nbsp;Siobhan S. McMahon","doi":"10.1016/j.jneumeth.2025.110499","DOIUrl":"10.1016/j.jneumeth.2025.110499","url":null,"abstract":"<div><h3>Background</h3><div><em>Ex vivo</em> organotypic spinal cord slice culture (OSC) models are advantageous for spinal cord injury (SCI) research. They retain <em>in vivo</em> cellular interactions but involve fewer ethical concerns and are easier to manipulate than <em>in vivo</em> models. Due to improved viability OSCs derived from early postnatal animals are preferred. A transection injury OSC model derived from postnatal day (P) 4 rats has been validated, however as rodent spinal cord development changes significantly in early postnatal weeks, older postnatal day animals may be more appropriate for OSC models.</div></div><div><h3>New method</h3><div>This study compared the viability of OSCs derived from P11 rats with the established P4 transection model.</div></div><div><h3>Results</h3><div>No significant differences in cell viability were found between P4 and P11 control slices, or between control and injured slices within each age group. P11 OSCs also demonstrated strong viable cell ingrowth into a transection injury gap post-injury, comparable to P4 OSCs, and increased their volume fraction of immunostaining for the glial scar markers GFAP and CSPGs along transection gap edges. Sex was not found to affect any of these parameters.</div></div><div><h3>Comparison with existing methods</h3><div>Our findings demonstrate that P11 OSCs maintain viability and cellular ingrowth post transection injury comparable to that of the well-established P4 derived OSCs. P11 OSCs were also found to exhibit a glial scarring response post transection injury.</div></div><div><h3>Conclusions</h3><div>OSCs from P11 animals maintain viability comparable to P4 OSCs while also exhibiting a scarring response. This study concludes that P11 OSCs are a valid alternate to P4 OSC transection SCI model.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110499"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain MRIs classification based on 2D SWD-MF-DFA","authors":"Jing Wang ,&nbsp;Xinpei Wu ,&nbsp;Haozhe Wang ,&nbsp;Jian Wang","doi":"10.1016/j.jneumeth.2025.110468","DOIUrl":"10.1016/j.jneumeth.2025.110468","url":null,"abstract":"<div><h3>Background:</h3><div>To improve imaging classification accuracy, we modify the traditional 2D multifractal trend fluctuation analysis (MF-DFA) method to better preserve local feature values. Inspired by MF-DFA, we develop a novel method for extracting eigenvalues, enhancing the precision of imaging analysis.</div></div><div><h3>New method:</h3><div>In this paper, we propose an enhanced algorithm building upon the traditional 2D MF-DFA. Our approach introduces a 2D sliding window (SWD) technique for feature value extraction. Initially, the local generalized Hurst index of the imaging is derived using the SWD algorithm, based on MF-DFA principles. Subsequently, the generalized Hurst index is recalculated for the digital matrix formed by these local Hurst indexes. These vectors are then input into a support vector machine (SVM) for classification. This methodology seeks to refine the traditional 2D MF-DFA by more effectively preserving local feature values in imaging.</div></div><div><h3>Results:</h3><div>The classification accuracy of the SWD eigenvalue extraction method based on 2D MF-DFA reaches 91.54%.</div></div><div><h3>Comparison with existing methods:</h3><div>We employ brain magnetic resonance imaging (MRI) data sets to evaluate the efficacy of both the conventional 2D MF-DFA method and our proposed feature value extraction technique. Both methods are applied alongside a SVM for classification. The findings reveal that the conventional 2D MF-DFA method yields a classification accuracy of 59.40%, while our SWD feature value extraction method attains a classification accuracy of 91.54%.</div></div><div><h3>Conclusion:</h3><div>This substantial performance enhancement underscores the superiority of the SWD approach over the conventional method.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"421 ","pages":"Article 110468"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}