Journal of Neuroscience Methods最新文献

{"title":"Aseptic, semi-sealed cranial chamber implants for chronic multi-channel neurochemical and electrophysiological neural recording in nonhuman primates","authors":"Jiwon Choi ,&nbsp;Usamma Amjad ,&nbsp;Raymond Murray ,&nbsp;Ritesh Shrivastav ,&nbsp;Tobias Teichert ,&nbsp;Baldwin Goodell ,&nbsp;Matthew Olson ,&nbsp;David J. Schaeffer ,&nbsp;Julia K. Oluoch ,&nbsp;Helen N. Schwerdt","doi":"10.1016/j.jneumeth.2025.110467","DOIUrl":"10.1016/j.jneumeth.2025.110467","url":null,"abstract":"<div><h3>Background</h3><div>Invasive electrophysiological recordings in subcortical structures of nonhuman primates typically involve implanting electrodes into the brain through a skull-mounted chamber. These electrodes may be attached to the chamber temporarily for hours of neural recording, or permanently for long-term studies. Current challenges involve maintaining asepsis and integrating dual-modality monitoring of both electrical and chemical neural activity.</div></div><div><h3>New method</h3><div>We developed an implantable neural interface that provides such dual-modality monitoring in monkeys, while maintaining aseptic conditions for year-long periods. We leveraged osseointegrating materials and hermetic sealing strategies to prevent the transmission of pathogenic species, while preserving the modular functionality of chamber systems, such as sensor depth adjustability. The system also features an aspirating port for culturing chamber fluid to ensure continued asepsis.</div></div><div><h3>Results</h3><div>Our chamber system was shown to provide successful recordings of dopamine and electrical neural activity in two monkeys while maintaining negative bacteria culture results for over a year post-implantation.</div></div><div><h3>Comparison with existing methods</h3><div>Sealed chamber systems prevent contamination and reduce the risk of compromising animal health by minimizing the accumulation of pathogenic organisms. Such sealed chambers also eliminate the need for frequent cleaning. However, neurochemical measurements require specialized electrodes with fragile carbon fiber tips and are not compatible with recently developed, sealed chamber systems.</div></div><div><h3>Conclusion</h3><div>This advanced chamber design builds upon traditional chamber protocols to enable chronic measurements of chemical and electrical neural activity. This approach facilitates novel ways to study the brain in behaving primates while prioritizing the long-term health and welfare of the animals.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110467"},"PeriodicalIF":2.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in endovascular brain computer interface: Systematic review and future implications","authors":"Julien Ognard ,&nbsp;Gerard El Hajj ,&nbsp;Onam Verma ,&nbsp;Sherief Ghozy ,&nbsp;Ramanathan Kadirvel ,&nbsp;David F. Kallmes ,&nbsp;Waleed Brinjikji","doi":"10.1016/j.jneumeth.2025.110471","DOIUrl":"10.1016/j.jneumeth.2025.110471","url":null,"abstract":"<div><h3>Background</h3><div>Brain-computer interfaces (BCIs) translate neural activity into real-world commands. While traditional invasive BCIs necessitate craniotomy, endovascular BCIs offer a minimally invasive alternative using the venous system for electrode placement.</div></div><div><h3>New method</h3><div>This systematic review evaluates the technical feasibility, safety, and clinical outcomes of endovascular BCIs, discussing their future implications. A systematic review was conducted per PRISMA guidelines. The search spanned PubMed, Web of Science, and Scopus databases using keywords related to neural interfaces and endovascular approaches. Studies were included if they reported on endovascular BCIs in preclinical or clinical settings. Dual independent screening and extraction focused on electrode material, recording capabilities, safety parameters, and clinical efficacy.</div></div><div><h3>Results</h3><div>From 1385 initial publications, 26 met the inclusion criteria. Seventeen studies investigated the Stentrode device. Among the 24 preclinical studies, 16 used ovine or rodent models, and 9 addressed engineering or simulation aspects. Two clinical studies reported six ALS patients successfully using an endovascular BCI for digital communication. Preclinical data established the endovascular ovine model, demonstrating stable neural recordings and vascular changes with long-term implantation. Key challenges include thrombosis risk, long-term electrode stability, and anatomical variability.</div></div><div><h3>Comparison with existing methods</h3><div>Endovascular BCI reduced invasiveness, improved safety profiles, with comparable neural recording fidelity to invasive methods, and promising preliminary clinical outcomes in severely paralyzed patients.</div></div><div><h3>Conclusions</h3><div>Early results are promising, but clinical data remain scarce. Further research is needed to optimize signal processing, enhance electrode biocompatibility, and refine endovascular procedures for broader clinical applications.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110471"},"PeriodicalIF":2.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spike detection of human sympathetic nerve activity using wavelet transformation and Valsalva maneuver denoising","authors":"Surat Kulapatana ,&nbsp;Stefano Rigo ,&nbsp;Vasile Urechie ,&nbsp;Robert J. Brychta ,&nbsp;Raffaello Furlan ,&nbsp;Italo Biaggioni ,&nbsp;André Diedrich","doi":"10.1016/j.jneumeth.2025.110482","DOIUrl":"10.1016/j.jneumeth.2025.110482","url":null,"abstract":"<div><h3>Background</h3><div>Sympathetic function is directly assessed by microneurography measuring muscle sympathetic nerve activity (MSNA). The recordings are typically corrupted with noise and require denoising. We aim to estimate microneurographic noise individually from physiologically suppressed MSNA during Valsalva phase 4 (VM4).</div></div><div><h3>New method</h3><div>We developed MSNA adaptive processing (MAP). MSNA recordings during Valsalva were transformed by stationary wavelet transformation. Level-specific noise thresholds were computed from 4 SD of detail coefficients from VM4 and were implemented for denoising. The denoised signals were inverse transformed, then the MSNA spikes were detected.</div><div>We compared detection performance of the MAP with the current two-stage kurtosis method in simulated MSNA signals, and recordings from 17 healthy and 19 postural orthostatic tachycardia syndrome (POTS) female subjects performing Valsalva.</div></div><div><h3>Results</h3><div>The MAP had higher correct detections of MSNA spikes than the kurtosis method in simulated signals wit high burst rate (50 burst/min) and low signal-to-noise ratio (SNR =2) (MAP vs kurtosis; 23.81 ± 15.49 % vs 16.98 ± 12.75 %, p &lt; 0.001). The improvement was confirmed by shorter error distance of the precision-recall plot (0.535 ± 0.175 vs 0.542 ± 0.177, p = 0.011).</div><div>The MAP detected higher spike rate during VM phase 2 in healthy (24.11 ± 9.85 vs 19.57 ± 8.60 spike/s, p = 0.049), but non-significant in POTS (24.19 ± 13.70 vs 20.30 ± 11.85 spike/s, p = 0.101).</div></div><div><h3>Comparison with existing methods</h3><div>The detection performance of the MAP is superior to the current two-stage kurtosis method.</div></div><div><h3>Conclusions</h3><div>The proposed MAP method individually estimating noise from VM4 could improve MSNA spike detection, compared with the kurtosis method. The advantages are most prominent in high burst rate and low SNR MSNA recordings.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110482"},"PeriodicalIF":2.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A subject transfer neural network fuses Generator and Euclidean alignment for EEG-based motor imagery classification","authors":"Chengqiang Xie,&nbsp;Li Wang,&nbsp;Jiafeng Yang,&nbsp;Jiaying Guo","doi":"10.1016/j.jneumeth.2025.110483","DOIUrl":"10.1016/j.jneumeth.2025.110483","url":null,"abstract":"<div><h3>Background</h3><div>Brain-computer interface (BCI) facilitates the connection between human brain and computer, enabling individuals to control external devices indirectly through cognitive processes. Although it has great development prospects, the significant difference in EEG signals among individuals hinders users from further utilizing the BCI system.</div></div><div><h3>New method</h3><div>Addressing this difference and improving BCI classification accuracy remain key challenges. In this paper, we propose a transfer learning model based on deep learning to transfer the data distribution from the source domain to the target domain, named a subject transfer neural network combining the Generator with Euclidean alignment (ST-GENN). It consists of three parts: 1) Align the original EEG signals in the Euclidean space; 2) Send the aligned data to the Generator to obtain the transferred features; 3) Utilize the Convolution-attention-temporal (CAT) classifier to classify the transferred features.</div></div><div><h3>Results</h3><div>The model is validated on BCI competition IV 2a, BCI competition IV 2b and SHU datasets to evaluate its classification performance, and the results are 82.85 %, 86.28 % and 67.2 % for the three datasets, respectively.</div></div><div><h3>Comparison with existing methods</h3><div>The results have been shown to be robust to subject variability, with the average accuracy of the proposed method outperforming baseline algorithms by ranging from 2.03 % to 15.43 % on the 2a dataset, from 0.86 % to 10.16 % on the 2b dataset and from 3.3 % to 17.9 % on the SHU dataset.</div></div><div><h3>Conclusions for research articles</h3><div>The advantage of our model lies in its ability to effectively transfer the experience and knowledge of the source domain data to the target domain, thus bridging the gap between them. Our method can improve the practicability of MI-BCI systems.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110483"},"PeriodicalIF":2.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of non-invasive brain stimulation combined with functional magnetic resonance imaging in post-stroke motor function rehabilitation.","authors":"Xiaoli Zou, Jiaju Zhu, Song Hu, Zhen Hou, Guodong Ma","doi":"10.1016/j.jneumeth.2025.110470","DOIUrl":"https://doi.org/10.1016/j.jneumeth.2025.110470","url":null,"abstract":"<p><strong>Background: </strong>Repetitive Transcranial Magnetic Stimulation (rTMS) is a promising non-invasive brain stimulation (NIBS) system for post-stroke motor rehabilitation. However, its underlying mechanisms are still not well understood.</p><p><strong>New method: </strong>In this research, 170 post-stroke patients with motor impairments were randomly divided into an rTMS intervention group (n = 85) and a control group (n = 85). Along with routine motor rehabilitation exercises, the rTMS group received 30minutes of 5Hz rTMS over the left Dorsolateral Prefrontal Cortex (DPC) three times a week for 30 days. Sham rTMS treatment can be allocated to the control group. Resting-state functional magnetic resonance imaging (rs-fMRI) was used to evaluate brain activity and functional connectivity (FC) in motor-related areas.</p><p><strong>Results: </strong>Both the intervention and control groups showed significant motor function improvements, but the rTMS group had more substantial gains. In the intervention group, FCA values in motor regions such as the primary motor cortex (PMC) and Precentral Gyrus (PCG) improved. There was also an increase in FC between the DPC and motor areas.</p><p><strong>Comparison with existing methods: </strong>Unlike some previous studies that may have focused only on motor function improvement without in-depth exploration of brain activity and connectivity changes, this study used rs-fMRI to comprehensively analyze the cerebral functional alterations induced by rTMS, providing a more detailed understanding of the underlying mechanisms of rTMS in post-stroke rehabilitation.</p><p><strong>Conclusions: </strong>The findings suggest that rTMS promotes motor recovery in post-stroke patients by modulating brain activity and connectivity within motor networks.</p>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110470"},"PeriodicalIF":2.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serendipitous discovery of intrathecal catheterization via the lumbosacral plexus in rats: A preliminary study","authors":"Atsushi Saiga ,&nbsp;Takeshi Suzuki ,&nbsp;Yadong Shi ,&nbsp;Kenkichi Michimoto ,&nbsp;Kentaro Yamada ,&nbsp;Todd Graham ,&nbsp;Khashayar Farsad","doi":"10.1016/j.jneumeth.2025.110472","DOIUrl":"10.1016/j.jneumeth.2025.110472","url":null,"abstract":"<div><h3>Background</h3><div>Intrathecal catheterization is essential for experimental studies, including drug delivery, stem cell therapy, and gene therapy, but it can sometimes be quite costly. Since traditional techniques, such as acute needle puncture and catheterization with or without laminectomy have shown varying success rates and procedural challenges, there is room for improvement.</div></div><div><h3>New method</h3><div>This study presents an alternative approach to intrathecal catheterization via the lumbosacral plexus, which was serendipitously discovered during an attempt to cannulate the lymphatic system in male Lewis rats using a 0.010-inch microguidewire and a 1.3-F microcatheter with an over-the-wire technique under fluoroscopic guidance.</div></div><div><h3>Results</h3><div>Intrathecal catheterization via the left L3 spinal nerve was achieved in 14 out of 17 rats (82 %). In one case, only the microguidewire was inserted, and in two cases, spinal nerve cannulation was unsuccessful. The median catheterization duration was 20 minutes. Accidental intrathecal placement was confirmed by postmortem micro-CT with iodinated contrast and dissection following isosulfan blue staining. No cerebrospinal fluid leakage was observed fluoroscopically during the procedure.</div></div><div><h3>Comparison with existing methods</h3><div>This method demonstrated a higher cannulation rate and a procedural time comparable to traditional techniques such as non-laminectomized catheterization. Additionally, it eliminates the risk of cerebrospinal fluid leakage, a common complication in conventional catheterization approaches. However, motor function impairment due to nerve injury and the need for specialized fluoroscopy settings remain limitations.</div></div><div><h3>Conclusions</h3><div>This serendipitous discovery suggests that intrathecal catheterization via the lumbosacral plexus may be a feasible alternative approach in select cases.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"419 ","pages":"Article 110472"},"PeriodicalIF":2.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gait recognition based on sEMG signal using progressive feature selection method","authors":"Chuanjiang Li ,&nbsp;Xinhao Ding ,&nbsp;Jiajun Tu ,&nbsp;Ang Li ,&nbsp;Yanfei Zhu ,&nbsp;Ya Gu ,&nbsp;Erlei Zhi","doi":"10.1016/j.jneumeth.2025.110469","DOIUrl":"10.1016/j.jneumeth.2025.110469","url":null,"abstract":"<div><h3>Background</h3><div>Gait recognition based on surface electromyography (sEMG) signals has many applications in exoskeleton control. However, due to the irrelevance and redundancy of its features, how to extract features effectively and improve the recognition accuracy is a hotspot of current research.</div></div><div><h3>New method</h3><div>This study proposes a progressive feature selection (PFS) gait recognition method based on sEMG. First, to solve the problem of inaccurate gait description, the stereo modelling projection and 3D dynamic capture are fused to capture the time and frequency domain features derived from the four muscles of the human lower limb according to the gait phase. Then, to address the problem of poor gait classification accuracy, a progressive feature combination optimization is performed based on the fitness evaluation to preserve the key information embedded in the features while eliminating features that contribute less to the model accuracy. Therefore, model accuracy is improved by determining the best combination of features.</div></div><div><h3>Results</h3><div>The progressive feature selection method shows considerable performance in sEMG-based gait recognition, with the average accuracy of 98.54 % and the median accuracy of 98.67 %.</div><div>Comparison with existing methods: In order to verify the effectiveness of the proposed algorithm more comprehensively, the practical experimental dataset and the publicly available SIAT-LLMD dataset are adopted respectively. Compared with the state-of-the-art methods, the gait recognition accuracy of the proposed PFS algorithm can reach 98.91 % and 98.54 %.</div></div><div><h3>Conclusions</h3><div>The proposed PFS gait recognition method can significantly reduce unnecessary features, thus improving the recognition accuracy and safety of lower limb exoskeleton robots.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"419 ","pages":"Article 110469"},"PeriodicalIF":2.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Labeling phrenic afferents with intrapleural AAV-PHP.S","authors":"Amanda C. Morales ,&nbsp;Taylor C. Holmes ,&nbsp;Felix T. Sanchez ,&nbsp;Haozhi Huang ,&nbsp;Jordan J. Williams ,&nbsp;Kristi A. Streeter","doi":"10.1016/j.jneumeth.2025.110466","DOIUrl":"10.1016/j.jneumeth.2025.110466","url":null,"abstract":"<div><h3>Background</h3><div>Understanding the role of musculoskeletal afferents in health and disease relies on the ability to selectively label afferents. Traditional approaches involve using adeno-associated viral (AAV) tools to transduce afferents with intrathecal, intramuscular, or direct dorsal root ganglion (DRG) injections. However, these approaches are surgically invasive, have non-specific labeling, or do not target functional groups of afferents. For example, labeling phrenic afferents arising from the diaphragm muscle is challenging due to the presence of musculoskeletal and cutaneous afferents from the forelimb, neck, and shoulder in the C3-C5 DRGs.</div></div><div><h3>New method</h3><div>Using a new capsid variant of AAV9 with enhanced tropism toward afferents (AAV-PHP.S), we investigated if intrapleural injection of AAV-PHP.S transduces phrenic afferents in the cervical DRGs and spinal cord.</div></div><div><h3>Results</h3><div>In animals receiving AAV-PHP.S, we observed robust tdTomato labeling in the DRGs, dorsal roots, dorsal columns, and spinal projections throughout the spinal gray matter. We did not see the same pattern of afferent labeling when we transected the phrenic nerve prior to intrapleural injection, nor did we find any evidence for motor neuron labeling. Classification of labeled afferents suggests preferential labeling of large diameter proprioceptive neurons. Time-course experiments show tdTomato expression in DRG neurons plateaued by 2 weeks.</div></div><div><h3>Comparison with existing methods</h3><div>To our knowledge this is the first AAV-based method that preferentially targets phrenic afferents without also labeling phrenic motor neurons.</div></div><div><h3>Conclusions</h3><div>This approach labels phrenic afferents and may be used in combination with optogenetic or chemogenetic tools to advance our understanding of the functional role of phrenic afferents.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"419 ","pages":"Article 110466"},"PeriodicalIF":2.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juggler’s ASR: Unpacking the principles of artifact subspace reconstruction for revision toward extreme MoBI","authors":"Hyeonseok Kim ,&nbsp;Chi-Yuan Chang ,&nbsp;Christian Kothe ,&nbsp;John Rehner Iversen ,&nbsp;Makoto Miyakoshi","doi":"10.1016/j.jneumeth.2025.110465","DOIUrl":"10.1016/j.jneumeth.2025.110465","url":null,"abstract":"<div><h3>Background</h3><div>To improve the Artifact Subspace Reconstruction (ASR) algorithm's performance for real-world EEG data by addressing the problem of low-quality or no calibration data identification in the original ASR (ASR_original) algorithm.</div></div><div><h3>New method</h3><div>We proposed a new method for defining high-quality calibration data using point-by-point amplitude evaluation to eliminate collateral rejection of clean data, which is identified as the major cause of the problem with ASR_original. We compared non-parametric and parametric approaches, namely Density-Based Spatial Clustering of Applications with Noise (DBSCAN) and the Generalized Extreme Value (GEV) distribution (ASR_DBSCAN and ASR_GEV, respectively).</div></div><div><h3>Results (Comparison with existing methods)</h3><div>We demonstrated the effectiveness of these approaches on simulated and real EEG data. Simulation results showed that ASR_DBSCAN and ASR_GEV removed simulated artifacts completely where ASR_original failed, both in time- and frequency-domain evaluations. In empirical data from 205-channel EEG recordings during a three-ball juggling task (n = 13), ASR_DBSCAN found 42 % and ASR_GEV found 24 % of data usable for calibration on average, compared to only 9 % by ASR_original. Subsequent Independent Component Analysis (ICA) showed that data preprocessed with ASR_DBSCAN and ASR_GEV produced brain ICs that accounted for more variance of the original data (30 % and 29 %) compared to ASR_original (26 %).</div></div><div><h3>Conclusions</h3><div>The proposed ASR_DBSCAN and ASR_GEV methods handle motion-related artifacts better than the original ASR algorithm, enabling researchers to better extract brain activity during real-world motor tasks. These methods provide a practical advantage in processing EEG data from experiments involving high-intensity motor activities, advancing biomedical research capabilities.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"420 ","pages":"Article 110465"},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcranial magnetic stimulation-based neuroplasticity in the treatment of amblyopia","authors":"Yilong Lin ,&nbsp;Kaifang Cai","doi":"10.1016/j.jneumeth.2025.110464","DOIUrl":"10.1016/j.jneumeth.2025.110464","url":null,"abstract":"<div><h3>Background</h3><div>In recent years, repetitive transcranial magnetic stimulation (rTMS) has shown positive clinical effects in improving neuroplasticity by modulating cortical neural activity, particularly the functional connectivity of visual-related brain regions. This research was aimed to investigate the effects of rTMS on visual function in adult amblyopia and to assess changes in brain neuronal activity before and following remedy using resting-state functional magnetic resonance imaging (rs-fMRI).</div></div><div><h3>New Method</h3><div>A total of 148 patients with anisometropic amblyopia were enrolled and randomly divided into: intraocular lens (ICL) group and ICL+rTMS group, with 74 cases in each group. All patients received detailed perioperative care. Best-corrected visual acuity (BCVA) and random dot stereopsis were assessed using the Vision Perception Test System before treatment and 3 months following remedy, and brain functional status was evaluated using rs-fMRI.</div></div><div><h3>Results</h3><div>As against pre-treatment levels, both ICL group and ICL+rTMS group suggested visible improvements in BCVA and random dot stereopsis 3 months following remedy, with ICL+rTMS group exhibiting better outcomes than ICL group (P &lt; 0.05). The rs-fMRI revealed distinct patterns of neural plasticity: ICL group exhibited an increase in the ALFF of the ipsilateral frontal lobe, while ICL+rTMS group showed a decrease in the ALFF of the same-side cerebellum (P &lt; 0.05). Compared to ICL alone, the combination of rTMS and ICL significantly reduced the ReHo in the ipsilateral frontal lobe and superior frontal gyrus, decreased the fALFF in the contralateral temporal lobe, and increased the fALFF in the contralateral occipital lobe (P &lt; 0.05).</div></div><div><h3>Comparison with Existing Methods</h3><div>The application of rTMS to directly regulate neural plasticity provides a non-invasive and precise treatment method. Compared with traditional therapies, rTMS can more effectively promote the reorganization of visual cortex function in amblyopia patients, improve treatment efficacy, and have fewer side effects, thus having high clinical application potential.</div></div><div><h3>Conclusion</h3><div>rTMS can effectively improve visual function in adult amblyopia patients by modulating neuronal activity and enhancing visual cortical neuroplasticity to correct the interocular excitation differences and exert therapeutic effects.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"419 ","pages":"Article 110464"},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}