Journal of Neuroscience Methods最新文献

An adaptive protocol to assess physiological responses as a function of task demand in speech-in-noise testing. 语音噪声测试中任务需求对生理反应的影响。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-31 DOI: 10.1016/j.jneumeth.2024.110348

Edoardo Maria Polo, Davide Simeone, Maximiliano Mollura, Alessia Paglialonga, Riccardo Barbieri

{"title":"An adaptive protocol to assess physiological responses as a function of task demand in speech-in-noise testing.","authors":"Edoardo Maria Polo, Davide Simeone, Maximiliano Mollura, Alessia Paglialonga, Riccardo Barbieri","doi":"10.1016/j.jneumeth.2024.110348","DOIUrl":"10.1016/j.jneumeth.2024.110348","url":null,"abstract":"Background: Acoustic challenges impose demands on cognitive resources, known as listening effort (LE), which can substantially influence speech perception and communication. Standardized assessment protocols for monitoring LE are lacking, hindering the development of adaptive hearing assistive technology.New method: We employed an adaptive protocol, including a speech-in-noise test and personalized definition of task demand, to assess LE and its physiological correlates. Features extracted from electroencephalogram, galvanic skin response, electrocardiogram, respiration, pupil dilation, and blood volume pulse responses were analyzed as a function of task demand in 21 healthy participants with normal hearing.Results: Heightened sympathetic response was observed with higher task demand, evidenced by increased heart rate, blood pressure, and breath amplitude. Blood volume amplitude and breath amplitude exhibited higher sensitivity to changes in task demand.Comparison with existing methods: Notably, galvanic skin response showed higher amplitude during low task demand phases, indicating increased attention and engagement, aligning with findings from electroencephalogram signals and Lacey's attention theory.Conclusions: The analysis of a range of physiological signals, spanning cardiovascular, central, and autonomic domains, demonstrated effectiveness in comprehensively examining LE. Future research should explore additional levels and manipulations of task demand, as well as the influence of individual motivation and hearing sensitivity, to further validate these outcomes and enhance the development of adaptive hearing assistive technology.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110348"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detecting fast-ripples on both micro- and macro-electrodes in epilepsy: A wavelet-based CNN detector. 检测癫痫微电极和大电极上的快速损伤：基于小波的 CNN 检测器。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-14 DOI: 10.1016/j.jneumeth.2024.110350

Ludovic Gardy, Jonathan Curot, Luc Valton, Louis Berthier, Emmanuel J Barbeau, Christophe Hurter

{"title":"Detecting fast-ripples on both micro- and macro-electrodes in epilepsy: A wavelet-based CNN detector.","authors":"Ludovic Gardy, Jonathan Curot, Luc Valton, Louis Berthier, Emmanuel J Barbeau, Christophe Hurter","doi":"10.1016/j.jneumeth.2024.110350","DOIUrl":"10.1016/j.jneumeth.2024.110350","url":null,"abstract":"Background: Fast-ripples (FR) are short (∼10 ms) high-frequency oscillations (HFO) between 200 and 600 Hz that are helpful in epilepsy to identify the epileptogenic zone. Our aim is to propose a new method to detect FR that had to be efficient for intracerebral EEG (iEEG) recorded from both usual clinical macro-contacts (millimeter scale) and microwires (micrometer scale).New method: Step 1 of the detection method is based on a convolutional neural network (CNN) trained using a large database of > 11,000 FR recorded from the iEEG of 38 patients with epilepsy from both macro-contacts and microwires. The FR and non-FR events were fed to the CNN as normalized time-frequency maps. Step 2 is based on feature-based control techniques in order to reject false positives. In step 3, the human is reinstated in the decision-making process for final validation using a graphical user interface.Results: WALFRID achieved high performance on the realistically simulated data with sensitivity up to 99.95 % and precision up to 96.51 %. The detector was able to adapt to both macro and micro-EEG recordings. The real data was used without any pre-processing step such as artefact rejection. The precision of the automatic detection was of 57.5. Step 3 helped eliminating remaining false positives in a few minutes per subject.Comparison with existing methods: WALFRID performed as well or better than 6 other existing methods.Conclusion: Since WALFRID was created to mimic the work-up of the neurologist, clinicians can easily use, understand, interpret and, if necessary, correct the output.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110350"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrode configurations for sensitive and specific detection of compound muscle action potentials to the tibialis anterior muscle after peroneal nerve injury in rats. 大鼠腓神经损伤后胫骨前肌复合肌动作电位敏感特异检测的电极配置。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-11-30 DOI: 10.1016/j.jneumeth.2024.110335

JuliAnne Allgood, Sam James, Lillian Laird, Albert Allotey, Jared Bushman

{"title":"Electrode configurations for sensitive and specific detection of compound muscle action potentials to the tibialis anterior muscle after peroneal nerve injury in rats.","authors":"JuliAnne Allgood, Sam James, Lillian Laird, Albert Allotey, Jared Bushman","doi":"10.1016/j.jneumeth.2024.110335","DOIUrl":"10.1016/j.jneumeth.2024.110335","url":null,"abstract":"Background: Quantifying peripheral nerve regeneration via electrophysiology is a commonly used technique, but it can be complicated by spurious electrical activity. This study sought to compare electrode configurations for measuring compound muscle action potential (CMAP) of the tibialis anterior (TA) muscle in a rat model for specific and sensitive detection of regeneration of peroneal nerve to the TA.New method: 10 Sprague-Dawley rats underwent a peroneal nerve transection with direct microsuture repair. CMAPs were conducted with different placements and types of electrodes. Compound action potentials (CAPs) and gait analysis were regularly collected up to 70 days (d) post operation (PO). Nerve sections were harvested at 49 d (n = 4) and 70 d (n = 6) PO and stained with toluidine blue to assess nerve morphometry.Results: Of the tested configurations for CMAPs, a concentric recording/reference electrode in combination with stimulation from the sciatic notch showed the least background and highest sensitivity, while some configurations showed significant noise and did not detect changes in CMAPs within the 70 d recording period following injury. CAPs, gait analysis, morphometry, and muscle mass support the extent of regeneration indicated by CMAPs collected with concentric electrodes.Conclusion: Collateral innervation patterns can complicate CMAP recordings as signals from adjacent muscles can be detected and misinterpreted as regeneration. The outcome of this study shows how differences in configurations and electrodes have significant effects on CMAP for the TA. The results identify methods using concentric electrodes that provide high specificity and sensitivity capable of detecting evidence of regeneration early after injury.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110335"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractal analysis to assess the differentiation state of oligodendroglia in culture. 分形分析评价培养少突胶质细胞分化状态。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-07 DOI: 10.1016/j.jneumeth.2024.110336

J Pascual-Guerra, M Torres-Rico, C L Paíno, J A Rodríguez-Navarro, A G García

{"title":"Fractal analysis to assess the differentiation state of oligodendroglia in culture.","authors":"J Pascual-Guerra, M Torres-Rico, C L Paíno, J A Rodríguez-Navarro, A G García","doi":"10.1016/j.jneumeth.2024.110336","DOIUrl":"10.1016/j.jneumeth.2024.110336","url":null,"abstract":"Background: Oligodendroglial development is accompanied by increased cell complexity. A simple and cost-effective evaluation of the pro-myelinating activity of different drugs and/or treatments would be of great interest. In cultured oligodendroglia, an evaluation of the pro-myelinating activity of different drugs and/or treatments can be achieved through fractal analysis, which allows measuring cell complexity.New method: Fractal dimension was assessed in two O4+ cell types (neural stem cell-derived and lineage-converted adipose tissue mesenchymal cells) under proliferating or differentiating conditions.Comparison with existing methods: This analysis, which was originally developed to analyze microglia, assigns a quantitative value (fractal dimension) to cellular profiles, obtaining higher coefficients as cells increase in size and arborizations instead of mRNA or protein quantification of mature oligodendroglial markers, such as MBP, MAG, O1 or PLP1/DM20.Results: This article describes a methodology to perform fractal analysis in immunofluorescent images of O4-positive (O4+) oligodendroglia using the FracLac plugin of ImageJ software. Pro-myelinating drug Benztropine-treated O4+ cells exhibit higher fractal dimension than control group.Conclusions: The results demonstrated the effectiveness and sensitivity of the fractal dimension coefficient provided by FracLac software to assess the effects of treatments on oligodendroglial differentiation.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110336"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An EEG-based emotion recognition method by fusing multi-frequency-spatial features under multi-frequency bands. 基于脑电图的多频段多频空间特征融合情感识别方法。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2025-01-06 DOI: 10.1016/j.jneumeth.2025.110360

Qiuyu Chen, Xiaoqian Mao, Yuebin Song, Kefa Wang

{"title":"An EEG-based emotion recognition method by fusing multi-frequency-spatial features under multi-frequency bands.","authors":"Qiuyu Chen, Xiaoqian Mao, Yuebin Song, Kefa Wang","doi":"10.1016/j.jneumeth.2025.110360","DOIUrl":"10.1016/j.jneumeth.2025.110360","url":null,"abstract":"Background: Recognition of emotion changes is of great significance to a person's physical and mental health. At present, EEG-based emotion recognition methods are mainly focused on time or frequency domains, but rarely on spatial information. Therefore, the goal of this study is to improve the performance of emotion recognition by integrating frequency and spatial domain information under multi-frequency bands.New methods: Firstly, EEG signals of four frequency bands are extracted, and then three frequency-spatial features of differential entropy (DE) symmetric difference (SD) and symmetric quotient (SQ) are separately calculated. Secondly, according to the distribution of EEG electrodes, a series of brain maps are constructed by three frequency-spatial features for each frequency band. Thirdly, a Multi-Parallel-Input Convolutional Neural Network (MPICNN) uses the constructed brain maps to train and obtain the emotion recognition model. Finally, the subject-dependent experiments are conducted on DEAP and SEED-IV datasets.Results: The experimental results of DEAP dataset show that the average accuracy of four-class emotion recognition, namely, high-valence high-arousal, high-valence low-arousal, low-valence high-arousal and low-valence low-arousal, reaches 98.71 %. The results of SEED-IV dataset show the average accuracy of four-class emotion recognition, namely, happy, sad, neutral and fear reaches 92.55 %.Comparison with existing methods: This method has a best classification performance compared with the state-of-the-art methods on both four-class emotion recognition datasets.Conclusions: This EEG-based emotion recognition method fused multi-frequency-spatial features under multi-frequency bands, and effectively improved the recognition performance compared with the existing methods.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110360"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing fMRI quality control. 加强功能磁共振成像质量控制。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-11-30 DOI: 10.1016/j.jneumeth.2024.110337

Lennard van den Berg, Nick Ramsey, Mathijs Raemaekers

{"title":"Enhancing fMRI quality control.","authors":"Lennard van den Berg, Nick Ramsey, Mathijs Raemaekers","doi":"10.1016/j.jneumeth.2024.110337","DOIUrl":"10.1016/j.jneumeth.2024.110337","url":null,"abstract":"Background: fMRI in clinical settings faces challenges affecting activity maps. Template matching can screen for abnormal results by providing an objective metric of activity map quality. This research tests how sample size, age, or gender-specific templates, and unilateral templates affect template matching results.New method: We used an fMRI database of 76 healthy subjects performing 7 tasks assessing motor, language, and working memory functions. Templates were created with varying numbers of subjects, genders, and ages. Individual subjects were compared to templates using leave-one-out cross validation. We also compared unilateral and bilateral templates.Results: Increasing sample size improved template matches, with diminishing returns for larger sample sizes. Gender and age-specific templates increased correlations for some tasks, with age having a larger effect than gender. Generally, templates including all subjects provided the highest correlations, indicating that age and gender effects did not outweigh the benefits of larger sample sizes. Unilateral templates of the task-dominant hemisphere increased template correlations.Conclusions: Age and gender affect templates, but the benefits depend on the database size. When the database is large enough, age and gender effects are beneficial. Unilateral templates enhance template matching, but practical benefits depend on the severity of neurological abnormalities in patients.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110337"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying decision making in rats using a contextual visual discrimination task: Detection and prevention of alternative behavioral strategies. 使用上下文视觉辨别任务研究大鼠的决策：发现和预防替代行为策略。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-10 DOI: 10.1016/j.jneumeth.2024.110346

Alon Amir, Drew B Headley, Mohammad M Herzallah, Asriya Karki, Ian T Kim, Denis Paré

{"title":"Studying decision making in rats using a contextual visual discrimination task: Detection and prevention of alternative behavioral strategies.","authors":"Alon Amir, Drew B Headley, Mohammad M Herzallah, Asriya Karki, Ian T Kim, Denis Paré","doi":"10.1016/j.jneumeth.2024.110346","DOIUrl":"10.1016/j.jneumeth.2024.110346","url":null,"abstract":"Background: The neural bases of decision-making and contextual sensory discriminations have traditionally been studied in primates, highlighting the role of the prefrontal cortex in cognitive control and flexibility. With the advent of molecular tools to manipulate and monitor neuronal activity, these processes have increasingly been studied in rodents. However, rodent tasks typically consist of two-alternative forced choice paradigms that usually feature coarse sensory discriminations and no contextual dependence, limiting prefrontal involvement in task performance.New method: To circumvent these limitations, we developed a novel contextual visual discrimination task that lends itself to rigorous psychophysical analyses. In this task, rats learn to detect left-right differences in one dimension (e.g. luminance or speed) depending on context while ignoring another (e.g. speed or luminance, respectively). Depending on trials, speed and luminance can be greater on the same side (congruent trials) or on opposite sides (incongruent trials).Results: Rats learned the task in four phases: nose-poking and lever-pressing (∼7 days), discriminating left-right differences in one dimension (∼20 days), discriminating left-right differences in a second dimension (∼10 days), and discriminating left-right differences in one of the two dimensions depending on context (∼2.5 months). A 20:80 ratio of congruent to incongruent trials is used to prevent rats from adopting alternative strategies.Comparison with existing methods: This task is comparable to contextual sensory discrimination tasks used in monkeys. Few equivalent tasks exist in rodents.Conclusions: This task will allow investigators to use the full neuroscientific armamentarium to study contextual neural coding in the rat prefrontal cortex.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110346"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A wireless optogenetic setup in freely moving mice for evaluation of cortical spreading depolarization in a chronic disease model. 无线光遗传学装置在自由运动小鼠中评估慢性疾病模型中皮质扩张性去极化。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2025-01-17 DOI: 10.1016/j.jneumeth.2025.110364

Annika Köhne, Simeon O A Helgers, Bettina Kewitz, Rieke M Haupt, Viktoria Oppermann, Franziska Meinert, Renan Sánchez-Porras, Maryam Said, Johannes Woitzik, Patrick Dömer

{"title":"A wireless optogenetic setup in freely moving mice for evaluation of cortical spreading depolarization in a chronic disease model.","authors":"Annika Köhne, Simeon O A Helgers, Bettina Kewitz, Rieke M Haupt, Viktoria Oppermann, Franziska Meinert, Renan Sánchez-Porras, Maryam Said, Johannes Woitzik, Patrick Dömer","doi":"10.1016/j.jneumeth.2025.110364","DOIUrl":"10.1016/j.jneumeth.2025.110364","url":null,"abstract":"Background: Spreading depolarization (SD) is an electrophysiological phenomenon of massive neuronal depolarization that occurs in a multitude of brain injuries. Clinical studies and experimental data have linked the occurrence of SDs with secondary brain damage. However, there is a translational gap because of methodological limitations between clinical and experimental approaches focusing on short-term effects. Moreover, usage of highly invasive SD triggers has put into question to what extent SDs themselves or the induction method had caused emergence of tissue damage.New method: To overcome this gap, we here show the successful realization of an experimental approach for long-term SD induction in a wireless setup of minimal invasive optogenetic stimulation in freely behaving mice.Results: The proposed method allows for reliable SD induction over the course of three weeks. SD characteristics induced with the wireless setup were comparable to SDs elicited by KCl or cable-bound optogenetic systems. Immunohistological analysis of c-Fos expression revealed neuronal depolarization across the stimulated hemisphere, whereas TUNEL staining revealed no stimulation related apoptosis.Comparison with existing methods: Optogenetic SD induction so far relied on cable- or fiber-bound systems which restrict experimental possibilities. The proposed model relies on wireless stimulation that allows SD induction in the home cage. In contrast to existing systems, the wireless setup also allows cage enrichment and group housing, therefore allowing behavioral analyses.Conclusion: This experimental setup has excellent potential to investigate the question of possible long-term SD effects in mouse models of different acute pathologies like traumatic brain injury or migraine.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110364"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using a mixed-reality headset to elicit and track clinically relevant movement in the clinic. 使用混合现实头戴式设备来诱导和跟踪临床相关的运动。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-14 DOI: 10.1016/j.jneumeth.2024.110349

Dylan Calame, Evan Lester, Phil Chiu, Lauren Seeberger

{"title":"Using a mixed-reality headset to elicit and track clinically relevant movement in the clinic.","authors":"Dylan Calame, Evan Lester, Phil Chiu, Lauren Seeberger","doi":"10.1016/j.jneumeth.2024.110349","DOIUrl":"10.1016/j.jneumeth.2024.110349","url":null,"abstract":"Background: 21st century neurology will require scalable and quantitative tools that can improve neurologic evaluations over telehealth and expand access to care. Commercially available mixed-reality headsets allow for simultaneous presentation of stimuli via holograms projected into the real world and objective and quantitative measurement of hand movement, eye movement, and phonation.New method: We created 6 tasks designed to mimic standard neurologic assessments and administered them to a single participant via the Microsoft HoloLens 2 mixed-reality headset. The tasks assessed postural hand tremor, finger tapping, pronation and supination of hands, hand and eye tracking of a center-out task, hand and eye tracking of a random motion task, and vocal assessment.Results: We show the utility of the HoloLens for commonly used neurological exams. First, we demonstrate that headset-derived holograms can project hand movements and objects in 3D space, providing a method to accurately and reproducibly present test stimuli to reduce test-test variability. Second, we found that participant hand movements closely matched holographic stimuli using a variety of metrics calculated on recorded movement data. Third, we showed that the HoloLens can record and playback exam tasks for visual inspection, sharing with other medical providers, and future analysis. Fourth, we showed that vocal recordings and analysis could be used to profile vocal characteristics over time. Together, this demonstrates the versatility of mixed reality headsets and possible applications for neurological assessment.Conclusions: Administering components of the neurologic exam via a self-contained and commercially available mixed-reality headset has numerous benefits including detailed kinematic quantification, reproducible stimuli presentation from test to test, and can be self-administered expanding access to neurological care and saving hospital time and money.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110349"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring persistence in animal models: The sinking platform test. 探索动物模型的持久性：沉降平台试验。

IF 2.7 4区医学

Journal of Neuroscience Methods Pub Date : 2025-03-01 Epub Date: 2024-12-15 DOI: 10.1016/j.jneumeth.2024.110352

Caterina Branca, Giulia Braccagni, Dario Finardi, Eleonora Corridori, Sara Salviati, Simona Scheggi, Marco Bortolato

{"title":"Exploring persistence in animal models: The sinking platform test.","authors":"Caterina Branca, Giulia Braccagni, Dario Finardi, Eleonora Corridori, Sara Salviati, Simona Scheggi, Marco Bortolato","doi":"10.1016/j.jneumeth.2024.110352","DOIUrl":"10.1016/j.jneumeth.2024.110352","url":null,"abstract":"Persistence is the capacity to sustain goal-oriented behavior despite recurring obstacles and setbacks. Recent studies have underscored the importance of this attribute as an integral facet of resilience and a protective factor against depression. In animal models, persistence is commonly examined through operant paradigms, wherein it is operationalized as resistance to the extinction of reward-directed actions. However, these methods are labor-intensive and resource-demanding, prompting questions about their efficiency in exploring the biological underpinnings of persistence and evaluating pharmacological interventions. To address these challenges, our team developed the Sinking Platform Test (SPT), a high-throughput animal task designed to assess persistence under stressful conditions. In the SPT, mice are trained to escape from a water-filled tank by climbing onto a platform above the water. Training also encompasses occasional \"failure trials\", where the platform is submerged after being climbed, compelling the mice to locate and ascend a new platform. The final test consists of a 5-minute session exclusively comprising failure trials, and persistence is measured as the number of climbed platforms. Our research revealed that chronic stress diminishes performance in the SPT, an effect reversed by chronic antidepressant treatment or voluntary exercise. These findings highlight the potential of SPT for investigating persistence and exploring its role in resilience and depression. Ongoing efforts within our laboratory focus on refining the SPT to minimize stress while enhancing methodological rigor and reproducibility, notably through automation. Future research endeavors will aim to improve SPT's translational relevance by adapting the paradigm for human application, potentially leveraging virtual-reality technologies.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110352"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0