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A refined Golgi-Cox method for the staining of embryonic neurons in the mouse brain
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-19 DOI: 10.1016/j.jneumeth.2025.110432
Benjamin Mitchell , Cooper Atterton , Darryl Whitehead , Stefan Thor , Michael Piper
{"title":"A refined Golgi-Cox method for the staining of embryonic neurons in the mouse brain","authors":"Benjamin Mitchell ,&nbsp;Cooper Atterton ,&nbsp;Darryl Whitehead ,&nbsp;Stefan Thor ,&nbsp;Michael Piper","doi":"10.1016/j.jneumeth.2025.110432","DOIUrl":"10.1016/j.jneumeth.2025.110432","url":null,"abstract":"<div><div>The Golgi-Cox stain remains a valuable technique used to investigate the morphology of individual neurons. Despite this, Golgi-Cox staining protocols are predominantly designed to impregnate adult neurons. Protocols optimised for the staining of immature embryonic mouse neurons have been previously developed but have limitations, including being time-consuming and being reliant on the use of expensive commercial kits. Here, we present a simple and inexpensive method for Golgi-Cox staining of embryonic neurons in the mouse brain. We identified that a 60 minute, 4 % paraformaldehyde (PFA) brain fixation step, followed by a wash with distilled water prior to immersion in Golgi-Cox solution was critical to the success of the stain. By altering the duration of the wash step, the visualisation of different populations across the neuraxis of neurons could be emphasised. Shorter washes enabled cortical neurons to be readily distinguished, whereas extending the wash steps was needed to enable subcortical neurons to be delineated.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110432"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnetic resonance spectroscopy and the menstrual cycle: A multi-centre assessment of menstrual cycle effects on GABA & GSH
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-19 DOI: 10.1016/j.jneumeth.2025.110430
Yulu Song , James J. Prisciandaro , Dace Apšvalka , Mae Bernard , Adam Berrington , Miguel Castelo-Branco , Mark K. Britton , Marta M. Correia , Koen Cuypers , Aleksandra Domagalik , Ulrike Dydak , Niall W. Duncan , Gerard E. Dwyer , Tao Gong , Ian Greenhouse , Katarzyna Hat , Melina Hehl , Shiori Honda , Chris Horton , Steve C.N. Hui , Katherine Dyke
{"title":"Magnetic resonance spectroscopy and the menstrual cycle: A multi-centre assessment of menstrual cycle effects on GABA & GSH","authors":"Yulu Song ,&nbsp;James J. Prisciandaro ,&nbsp;Dace Apšvalka ,&nbsp;Mae Bernard ,&nbsp;Adam Berrington ,&nbsp;Miguel Castelo-Branco ,&nbsp;Mark K. Britton ,&nbsp;Marta M. Correia ,&nbsp;Koen Cuypers ,&nbsp;Aleksandra Domagalik ,&nbsp;Ulrike Dydak ,&nbsp;Niall W. Duncan ,&nbsp;Gerard E. Dwyer ,&nbsp;Tao Gong ,&nbsp;Ian Greenhouse ,&nbsp;Katarzyna Hat ,&nbsp;Melina Hehl ,&nbsp;Shiori Honda ,&nbsp;Chris Horton ,&nbsp;Steve C.N. Hui ,&nbsp;Katherine Dyke","doi":"10.1016/j.jneumeth.2025.110430","DOIUrl":"10.1016/j.jneumeth.2025.110430","url":null,"abstract":"<div><h3>Background</h3><div>Gamma-aminobutyric acid (GABA) and glutathione (GSH) play a significant role in the functioning of a healthy brain and can both be quantified using magnetic resonance spectroscopy (MRS). Several small-scale studies have suggested MRS measured GABA may fluctuate with the menstrual cycle, but the effects on GSH are unknown. Utilising recent developments in MRS acquisition, this multi-lab study explores this issue across 4 distinctive brain regions.</div></div><div><h3>New methods</h3><div>Data were analysed from 12 independent sites from which a total of 30 women were scanned during three phases of their menstrual cycle corresponding to early follicular, ovulation and mid luteal phases. HERMES and HERCULES sequences were used to measure GABA and GSH in voxels located in the left motor cortex, left posterior insular, medial parietal and medial frontal. Linear mixed models were used to assess the variability contributed by site, participant and menstrual cycle phase.</div></div><div><h3>Results</h3><div>Similar variance was attributed to site and menstrual cycle phase for both GABA and GSH data. No systematic changes in GABA or GSH were revealed for any voxel as a consequence of menstrual cycle phase.</div></div><div><h3>Comparison with existing methods</h3><div>Despite our larger sample size and inclusion of more brain regions we fail to replicate previous findings of GABA change as a consequence of menstrual cycle phase. We also show for the first time that MRS measures of GSH so not significantly alter with cycle.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that the menstrual cycle has minimal impact on MRS measures of GABA and GSH. The presence of a menstrual cycle should not be used as justification for exclusion of women in MRS studies.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110430"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "An MRI protocol for anatomical and functional evaluation of the California sea lion brain" [J. Neurosci. Methods 353 (2021) 109097].
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-18 DOI: 10.1016/j.jneumeth.2025.110423
Peter F Cook, Vanessa A Hoard, Sudipto Dolui, Blaise deB Frederick, Richard Redfern, Sophie E Dennison, Barbie Halaska, Josh Bloom, Kris T Kruse-Elliott, Emily R Whitmer, Emily J Trumbull, Gregory S Berns, John A Detre, Mark D'Esposito, Frances M D Gulland, Colleen Reichmuth, Shawn P Johnson, Cara L Field, Ben A Inglis
{"title":"Corrigendum to \"An MRI protocol for anatomical and functional evaluation of the California sea lion brain\" [J. Neurosci. Methods 353 (2021) 109097].","authors":"Peter F Cook, Vanessa A Hoard, Sudipto Dolui, Blaise deB Frederick, Richard Redfern, Sophie E Dennison, Barbie Halaska, Josh Bloom, Kris T Kruse-Elliott, Emily R Whitmer, Emily J Trumbull, Gregory S Berns, John A Detre, Mark D'Esposito, Frances M D Gulland, Colleen Reichmuth, Shawn P Johnson, Cara L Field, Ben A Inglis","doi":"10.1016/j.jneumeth.2025.110423","DOIUrl":"https://doi.org/10.1016/j.jneumeth.2025.110423","url":null,"abstract":"","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110423"},"PeriodicalIF":2.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential mechanism and efficacy evaluation of transcranial focused ultrasound therapy for Alzheimer's disease
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-17 DOI: 10.1016/j.jneumeth.2025.110428
Wanqi Qin, Jiayi He, Yi Zhou
{"title":"Potential mechanism and efficacy evaluation of transcranial focused ultrasound therapy for Alzheimer's disease","authors":"Wanqi Qin,&nbsp;Jiayi He,&nbsp;Yi Zhou","doi":"10.1016/j.jneumeth.2025.110428","DOIUrl":"10.1016/j.jneumeth.2025.110428","url":null,"abstract":"<div><h3>Background</h3><div>Transcranial focused ultrasound (TFU) is emerging as a promising non-invasive therapy capable of blood-brain barrier (BBB) opening. TFU potentially allows the transfer of therapeutic agents to targeted brain areas for patients affected with Alzheimer's disease (AD).</div></div><div><h3>New method</h3><div>The efficacy and mechanism of TFU in modulating BBB permeability in key brain regions, including the hippocampus and frontal lobe, are investigated in this research. A total of 20 participants aged 60–85 years were involved with AD. The treatment protocol involved three TFU sessions, spaced three weeks apart. The research encompasses pre-assessment and post-assessment of treatment with follow-up ranging from 5 to 12 months.</div></div><div><h3>Results</h3><div>Statistical analysis involved paired t-tests to compare pre- and post-treatment cognitive scores, and ANOVA to predict significant differences in amyloid reduction across different brain regions, with the high decrease observed in the hippocampus. Multivariate Analysis (MANOVA) to explore the combined effect of cognitive and amyloid data. Linear Regression Analysis to predict cognitive improvement from amyloid plaque reduction. Longitudinal analysis for time-to-event analysis assessing the durability of effects over time.</div></div><div><h3>Comparison with existing methods</h3><div>Florbetaben Positron Emission Tomography (PET) scans demonstrated a reduction in β-amyloid plaque burden, with a 15 % average decrease in the treated brain regions. No adverse effects on disease progression were observed up to 1 year after treatment.</div></div><div><h3>Conclusion</h3><div>This analysis presents the largest cohort of AD patients treated with TFU, with the longest follow-up to date. The treatment demonstrated safety and feasibility, with reversible BBB opening in multiple brain regions.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110428"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in conduction velocities of nerve fibers excited by infrared and electrical stimulation
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-16 DOI: 10.1016/j.jneumeth.2025.110427
Louis Vande Perre , Javier Chávez Cerda , Soizic Gochard , Maxime Verstraeten , Romain Raffoul , Catherine Leonard , Jean Delbeke , Riëm El Tahry , Simon-Pierre Gorza , Antoine Nonclercq
{"title":"Differences in conduction velocities of nerve fibers excited by infrared and electrical stimulation","authors":"Louis Vande Perre ,&nbsp;Javier Chávez Cerda ,&nbsp;Soizic Gochard ,&nbsp;Maxime Verstraeten ,&nbsp;Romain Raffoul ,&nbsp;Catherine Leonard ,&nbsp;Jean Delbeke ,&nbsp;Riëm El Tahry ,&nbsp;Simon-Pierre Gorza ,&nbsp;Antoine Nonclercq","doi":"10.1016/j.jneumeth.2025.110427","DOIUrl":"10.1016/j.jneumeth.2025.110427","url":null,"abstract":"<div><h3>Background</h3><div>Infrared neural stimulation (INS) uses short optical pulses to activate nerves. While electrical stimulation (ES) activates large-diameter fibers first, light may preferentially activate small-diameter fibers first, which could be valuable for many clinical applications.</div></div><div><h3>New method</h3><div>This study used a compact diode laser of 1470 nm to perform INS. Conduction velocity (CV) measurements were performed to assess differences in fiber type activation between INS and ES in the rat sciatic nerve and the goat vagus nerve. The rat sciatic nerve was chosen as a standard model because of its well-characterized physiology and extensive use in studies of INS mechanisms. The goat vagus nerve was chosen because of its expected high proportion of small-diameter fibers and its larger size, which allows sufficient separation between recording units to optimize CNAP measurements.</div></div><div><h3>Results</h3><div>The results showed that in the rat sciatic nerve, ES-excited fibers had significantly higher CVs (9.81 ± 3.18 m/s) than INS-excited fibers (8.10 ± 2.82 m/s). In the goat vagus nerve, ES produced a mean CV of 6.47 ± 1.25 m/s, but INS did not produce clearly distinguishable compound nerve action potential, highlighting the challenges of applying INS to larger nerves.</div></div><div><h3>Comparison to existing methods</h3><div>To the best of our knowledge, CV is, for the first time, measured to identify the type of nerve fiber excited by INS.</div></div><div><h3>Conclusion</h3><div>These results suggest that INS may preferentially activate smaller diameter fibers, providing insight for potential neuromodulation applications.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110427"},"PeriodicalIF":2.7,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual-pathway EEG model with channel attention for virtual reality motion sickness detection
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-12 DOI: 10.1016/j.jneumeth.2025.110425
Chengcheng Hua , Yuechi Chen , Jianlong Tao , Zhian Dai , Wenqing Yang , Dapeng Chen , Jia Liu , Rongrong Fu
{"title":"Dual-pathway EEG model with channel attention for virtual reality motion sickness detection","authors":"Chengcheng Hua ,&nbsp;Yuechi Chen ,&nbsp;Jianlong Tao ,&nbsp;Zhian Dai ,&nbsp;Wenqing Yang ,&nbsp;Dapeng Chen ,&nbsp;Jia Liu ,&nbsp;Rongrong Fu","doi":"10.1016/j.jneumeth.2025.110425","DOIUrl":"10.1016/j.jneumeth.2025.110425","url":null,"abstract":"<div><h3>Background</h3><div>Motion sickness has been a key factor affecting user experience in Virtual Reality (VR) and limiting the development of the VR industry. Accurate detection of Virtual Reality Motion Sickness (VRMS) is a prerequisite for solving the problem.</div></div><div><h3>New method</h3><div>In this paper, a dual-pathway model with channel attention for detecting VRMS is proposed. The proposed model has two pathways that both consist of CNN blocks and channel attention modules. The first pathway takes the EEG signal as inputs. The second pathway transforms the EEG signal into brain networks of six frequency bands using Phase Locking Value (PLV) or ρ index (RHO) methods and takes the adjacent matrixes as input. The features from the two pathways are connected and fed into the fully connected layer for classification. Finally, a VR flight simulation experiment is performed and the EEG of the resting state before and after the virtual flight task are collected to validate the model.</div></div><div><h3>Results</h3><div>The average accuracy, precision, recall, and F1 score of the proposed model are 99.12 %, 99.12 %, 99.11 %, and 99.12 %, respectively.</div></div><div><h3>Comparison with existing methods</h3><div>Eight models are introduced as the reference methods and four of them are fused as the hybrid models in this study. The results show that the proposed model is better than those state-of-art models.</div></div><div><h3>Conclusions</h3><div>The proposed model outperforms the state-of-the-art models and provides objective and direct guidance for overcoming VRMS and optimizing VR experience.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110425"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-12 DOI: 10.1016/j.jneumeth.2025.110426
G. Lorenzo Odierna , Sarah Stednitz , April Pruitt , Joshua Arnold , Ellen J. Hoffman , Ethan K. Scott
{"title":"Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders","authors":"G. Lorenzo Odierna ,&nbsp;Sarah Stednitz ,&nbsp;April Pruitt ,&nbsp;Joshua Arnold ,&nbsp;Ellen J. Hoffman ,&nbsp;Ethan K. Scott","doi":"10.1016/j.jneumeth.2025.110426","DOIUrl":"10.1016/j.jneumeth.2025.110426","url":null,"abstract":"<div><h3>Background</h3><div>Zebrafish are a popular model system to study the genetic and neural basis of perception and behavior. Cultured primary neurons provide a complementary tool for such studies, but existing protocols for culturing embryonic zebrafish neurons are limited by short cell survival and low neuronal purity. In this study, we set out to establish a protocol to produce long lived brain cell cultures from zebrafish that could be used to study the mechanistic contributions of genes to neuronal networks.</div></div><div><h3>New method</h3><div>This protocol improves the viability of embryonic zebrafish primary brain cell cultures. We successfully optimized several parameters to generate long lived mixed cell type or pure neuronal cultures derived from embryonic zebrafish.</div></div><div><h3>Results</h3><div>Our optimized protocol produces cultures that form stable networks of neurons expressing the structural hallmarks of mature synaptic connections. As proof of principle, we apply our protocol to explore the cellular consequences of <em>scn1lab</em> loss of function. We find that loss of <em>scn1lab</em> results in increased prevalence of non-neuronal cells consistent with transcriptional signatures from embryonic tissue, providing support for the utility of our protocol.</div></div><div><h3>Comparison with existing method(s)</h3><div>Most existing embryonic zebrafish primary neuron culture protocols describe growing mixed cell types for short durations, with a reported maximum of 9 days <em>in vitro</em>. Here, we describe a protocol that produces cultures viable for over 100 days.</div></div><div><h3>Conclusions</h3><div>The protocol reported in this study raises embryonic zebrafish primary brain cell culture to similar standards observed by well-established methods using cell lines or mammalian tissue.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110426"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishing the electrophysiological feasibility of the rabbit median nerve as an experimental model for carpal tunnel syndrome
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-09 DOI: 10.1016/j.jneumeth.2025.110411
Semra Çınar , Erkut Turan , Ayşe Nur Akkoç
{"title":"Establishing the electrophysiological feasibility of the rabbit median nerve as an experimental model for carpal tunnel syndrome","authors":"Semra Çınar ,&nbsp;Erkut Turan ,&nbsp;Ayşe Nur Akkoç","doi":"10.1016/j.jneumeth.2025.110411","DOIUrl":"10.1016/j.jneumeth.2025.110411","url":null,"abstract":"<div><h3>Backround</h3><div>Rabbits are appropriate models for experimental carpal tunnel syndrome (CTS) studies. This study aimed to explore whether the distribution and innervation area of the nerves supplying the thenar muscles of rabbits are similar to those in humans using anatomical, electrophysiological, and histopathological methods.</div></div><div><h3>New method</h3><div>20 New Zealand rabbits were used to establish reference conduction values for the median and ulnar nerves. Median nerve denervation was performed on the left forelimb of six rabbits, and changes were assessed 33 days post-surgery. Normative data from healthy rabbits were compared with those from denervated rabbits, and comparisons were made between the right and left forelimbs of the denervated rabbits. Thenar and interosseous (2nd, 3rd, and 4th) muscles were used for histopathology. Dissections focused on the thenar muscles and branches of the median and ulnar nerves.</div></div><div><h3>Results</h3><div>Thenar muscles were 2.5–3 mm in width and 7–8 mm in length), innervated by both the median and ulnar nerves. Normative latency and amplitude values of 1.85 ± 0.30 ms and 7.71 ± 3.50 mV for the median nerve and 1.85 ± 0.19 ms and 6.14 ± 2.50 mV for the ulnar nerve, respectively. Denervation reduced thenar muscle CMAP amplitude (2.51 ± 2.16 mV, p = 0.047) on ulnar nerve stimulation, indicating dual innervation. The median sensory nerve conduction latency (2.55 ± 0.20 ms) was successfully performed for the first time. Histopathological analysis revealed localized atrophy and degenerative changes in the denervated thenar muscles.</div></div><div><h3>Comparison with existing methods</h3><div>The new method including novel normative data will significantly enhance the evaluation of CTS in experimental rabbit models, paving the way for more accurate and reliable future research.</div></div><div><h3>Conclusion</h3><div>The innervation patterns of rabbit thenar muscles were similar to those in humans. This data can aid CTS understanding in rabbit models.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110411"},"PeriodicalIF":2.7,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing therapeutic efficacy of fingolimod via intranasal delivery in an ethidium bromide-induced model of multiple sclerosis
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-08 DOI: 10.1016/j.jneumeth.2025.110415
Anshul Sharma , Nitin Sharma , Kushagra Khanna , Akshita Arora , Sidharth Mehan , Anjana Sharma
{"title":"Enhancing therapeutic efficacy of fingolimod via intranasal delivery in an ethidium bromide-induced model of multiple sclerosis","authors":"Anshul Sharma ,&nbsp;Nitin Sharma ,&nbsp;Kushagra Khanna ,&nbsp;Akshita Arora ,&nbsp;Sidharth Mehan ,&nbsp;Anjana Sharma","doi":"10.1016/j.jneumeth.2025.110415","DOIUrl":"10.1016/j.jneumeth.2025.110415","url":null,"abstract":"<div><h3>Background</h3><div>Multiple sclerosis (MS) is a chronic inflammatory autoimmune neurological characterized by muscle weakness, numbness, tingling, vision problems, and difficulty in coordination and balance caused by the damage of myelin content around the nerve fibres. The recent literature is evident that along with their lymphocyte attack prevention mechanism, fingolimod (FNG) can serve as neuroprotective also by ensuring their improved brain availability. Therefore, in this project brain availability of FNG was enhanced by delivering the FNG in the form of nanoparticles.</div></div><div><h3>New methods</h3><div>Fingolimod-loaded solid lipid nanoparticles (FNG-SLNs) were prepared using the solvent evaporation method and formulation factors (lipid concentration; X1, speed; X2, surfactant concentration; X3) and response were established by factorial design. FNG-SLNs were characterized for particle size, entrapment efficiency and i<em>n-vitro</em> drug release. Optimized formulations were characterized for <em>in-vivo</em> efficacy study in ethidium bromide-induced MS rat model.</div></div><div><h3>Result</h3><div>Obtained data revealed that the particle size and entrapment efficiency of FNG-SLNs optimized formulation was 125.4 nm and 79.86 % w/w respectively. <em>In-vitro</em> drug release study showed an initial burst release of the FNG up to 32.52 % in 30 min followed by sustained drug release up to 78.22 % in 24 h. Furthermore, <em>in-vivo</em> data of FNG-SLNs on ethidium bromide-induced MS rat model revealed better treatment response by showing several evidence such as signs of remyelination, restoration of neuron shape, and the recovered oligodendrocytes.</div></div><div><h3>Comparison with existing methods</h3><div>To the best of our knowledge this article demonstrates improved efficacy of FNG using SLNs.</div></div><div><h3>Conclusion</h3><div>This study demonstrates the successful development of FNG-SLNs to enhance the therapeutic efficacy of FNG for the treatment of multiple sclerosis.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110415"},"PeriodicalIF":2.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-view graph fusion of self-weighted EEG feature representations for speech imagery decoding
IF 2.7 4区 医学
Journal of Neuroscience Methods Pub Date : 2025-03-07 DOI: 10.1016/j.jneumeth.2025.110413
Zhenye Zhao , Yibing Li , Yong Peng , Kenneth Camilleri , Wanzeng Kong
{"title":"Multi-view graph fusion of self-weighted EEG feature representations for speech imagery decoding","authors":"Zhenye Zhao ,&nbsp;Yibing Li ,&nbsp;Yong Peng ,&nbsp;Kenneth Camilleri ,&nbsp;Wanzeng Kong","doi":"10.1016/j.jneumeth.2025.110413","DOIUrl":"10.1016/j.jneumeth.2025.110413","url":null,"abstract":"<div><h3>Background:</h3><div>Electroencephalogram (EEG)-based speech imagery is an emerging brain–computer interface paradigm, which enables the speech disabled to naturally and intuitively communicate with external devices or other people. Currently, speech imagery research decoding performance is limited. One of the reasons is that there is still no consensus on which domain features are more discriminative.</div></div><div><h3>New method:</h3><div>To adaptively capture the complementary information from different domain features, we treat each domain as a view and propose a multi-view graph fusion of self-weighted EEG feature representations (MVGSF) model by learning a consensus graph from multi-view EEG features, based on which the imagery intentions can be effectively decoded. Considering that different EEG features in each view have different discriminative abilities, the view-dependent feature importance exploration strategy is incorporated in MVGSF.</div></div><div><h3>Results:</h3><div>(1) MVGSF exhibits outstanding performance on two public speech imagery datasets (2) The learned consensus graph from multi-view features effectively characterizes the relationships of EEG samples in a progressive manner. (3) Some task-related insights are explored including the feature importance-based identification of critical EEG channels and frequency bands in speech imagery decoding.</div></div><div><h3>Comparison with existing methods:</h3><div>We compared MVGSF with single-view counterparts, other multi-view models, and state-of-the-art models. MVGSF achieved the highest accuracy, with average accuracies of 78.93% on the 2020IBCIC3 dataset and 53.85% on the KaraOne dataset.</div></div><div><h3>Conclusions:</h3><div>MVGSF effectively integrates features from multiple domains to enhance decoding capabilities. Furthermore, through the learned feature importance, MVGSF has made certain contributions to identify the EEG spatial-frequency patterns in speech imagery decoding.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"418 ","pages":"Article 110413"},"PeriodicalIF":2.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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