Journal of Mammary Gland Biology and Neoplasia最新文献

Developmental Regulation of circRNAs in Normal and Diseased Mammary Gland: A Focus on circRNA-miRNA Networks. 正常和病变乳腺中circrna的发育调控：circRNA-miRNA网络的研究

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-05-02 DOI: 10.1007/s10911-025-09580-w

Sarah Marei, Nour Maatouk, Mounir AbouHaidar, Rabih Talhouk

{"title":"Developmental Regulation of circRNAs in Normal and Diseased Mammary Gland: A Focus on circRNA-miRNA Networks.","authors":"Sarah Marei, Nour Maatouk, Mounir AbouHaidar, Rabih Talhouk","doi":"10.1007/s10911-025-09580-w","DOIUrl":"https://doi.org/10.1007/s10911-025-09580-w","url":null,"abstract":"Circular RNAs (circRNAs) have emerged as critical regulators in various biological processes including diseases. In the mammary gland (MG), which undergoes most of its development postnatally, circRNAs play pivotal roles in both physiological and pathological contexts. This review highlights the involvement of circRNAs during key developmental stages of the MG, with particular emphasis on lactation, where circRNA-miRNA networks significantly influence milk secretion and composition. CircRNAs exhibit stage-, breed- and species-specific expression patterns during lactation, which underscores their complexity. This intricate regulation also plays a significant role in pathological conditions of the MG, where dysregulated circRNA expression contributes to disease progression such as mastitis, early breast cancer (BC) stages, and epithelial-to-mesenchymal transition in BC (EMT). In mastitis, altered circRNA expression disrupts immune responses and compromises epithelial integrity. During early BC progression, circRNAs drive cell proliferation, while in EMT, they facilitate metastatic processes. By focusing on the circRNA-miRNA interactions underlying these processes, this review highlights their potential use as biomarkers for MG development, disease progression, and as therapeutic targets.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"8"},"PeriodicalIF":3.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development. STAT5B SH2结构域的疾病相关突变调节细胞因子驱动的增强子功能和乳腺发育

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-03-31 DOI: 10.1007/s10911-025-09582-8

Hye Kyung Lee, Jakub Jankowski, Chengyu Liu, Lothar Hennighausen

{"title":"Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development.","authors":"Hye Kyung Lee, Jakub Jankowski, Chengyu Liu, Lothar Hennighausen","doi":"10.1007/s10911-025-09582-8","DOIUrl":"10.1007/s10911-025-09582-8","url":null,"abstract":"Mammary gland development during pregnancy is controlled by lactogenic hormones via the JAK2-STAT5 pathway. Gene deletion studies in mice have revealed the crucial roles of both STAT5A and STAT5B in establishing the genetic programs necessary for the development of mammary epithelium and successful lactation. Several hundred single nucleotide polymorphisms (SNPs) have been identified in human STAT5B, although their pathophysiological significance remains largely unknown. The SH2 domain is vital for STAT5B activation, and this study focuses on the impact of two specific missense mutations identified in T cell leukemias, the substitution of tyrosine 665 with either phenylalanine (Y665F) or histidine (Y665H). By introducing these human mutations into the mouse genome, we uncovered distinct and opposite functions. Mice harboring the STAT5BY665H mutation failed to develop functional mammary tissue, resulting in lactation failure, while STAT5BY665F mice exhibited accelerated mammary development during pregnancy. Transcriptomic and epigenomic analyses identified STAT5BY665H as Loss-Of-Function (LOF) mutation, impairing enhancer establishment and alveolar differentiation, whereas STAT5BY665F acted as a Gain-Of-Function (GOF) mutation, elevating enhancer formation. Persistent hormonal stimulation through two pregnancies led to the establishment of enhancer structures, gene expression and successful lactation in STAT5BY665H mice. Lastly, we demonstrate that Olah, a gene known to drive life-threatening viral disease in humans, is regulated by STAT5B through a candidate four-partite super-enhancer. In conclusion, our findings underscore the role of human STAT5B variants in modulating mammary gland homeostasis and their critical impact on lactation.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"7"},"PeriodicalIF":3.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11958444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of ESR1, HER1, and HER2 Polymorphisms with Breast Cancer Risk in the KP Population, A Case-Control Study. 在KP人群中，ESR1、HER1和HER2多态性与乳腺癌风险的关联：一项病例对照研究

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-03-27 DOI: 10.1007/s10911-025-09581-9

Najeeb Ullah Khan, Hamza Khan, Abdullah R Alanzi, Tianhui Chen

{"title":"Association of ESR1, HER1, and HER2 Polymorphisms with Breast Cancer Risk in the KP Population, A Case-Control Study.","authors":"Najeeb Ullah Khan, Hamza Khan, Abdullah R Alanzi, Tianhui Chen","doi":"10.1007/s10911-025-09581-9","DOIUrl":"10.1007/s10911-025-09581-9","url":null,"abstract":"Breast cancer is a complex disease characterized by the uncontrolled growth of breast cells. Genetic variants in ESR1, HER1, and HER2 have been associated with breast cancer risk across different populations, with varying results. This study aimed to validate the association of ESR1 (rs2234693 and rs2046210), HER1 (rs11543848), and HER2 (rs1136201) variants with breast cancer risk in the KP population of Pakistan using a larger dataset. The study cohort included 528 patients with BC and 530 healthy controls. Blood samples were collected, and DNA was extracted using a non-enzymatic method. Genotyping was performed using the T-ARMS-PCR protocol. Our results for ESR1 (rs2234693) indicated a non-significant association between the mutant C allele (P = 0.102), TC (P = 0.1002), and CC genotype (P = 0.398) and breast cancer risk. In contrast, ESR1 and rs2046210 showed a significant association with the mutant A allele (P = 0.001), GA (P = 0.001), and AA genotype (P = 0.001), indicating an increased risk. HER1 and rs11543848 showed an increased risk of breast cancer, with the mutant allele A (P = 0.001), GA (P = 0.001), and AA genotype (P = 0.001). Similarly, alleles G (P = 0.004), AG (P = 0.001), and GG genotype (P = 0.003) of HER2 (rs1136201) were associated with higher breast cancer risk. Furthermore, ESR1 (rs2234693) was significantly associated with PR status, while both HER1 (rs11543848) and HER2 (rs1136201) were considerably associated with HER2 receptor status. In conclusion, this study explored the association of the selected variants of ESR1, HER1, and HER2 with breast cancer risk in the KP population using a larger data set, providing valuable insights into the genetic factors contributing to breast cancer risk and corresponding value added to breast cancer management.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"6"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Buenos Aires Breast Cancer Symposium (BA-BCS 2024) A Second Successful "Trial" for Bringing Together both World Hemispheres To Debate the Future of Translational Breast Cancer Research. 布宜诺斯艾利斯乳腺癌研讨会（BA-BCS 2024）第二次成功的“试验”，将两个半球聚集在一起，讨论转化性乳腺癌研究的未来。

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-03-15 DOI: 10.1007/s10911-025-09577-5

Claudia Lanari, Virginia Novaro, Mario Rossi, Edith C Kordon

引用次数: 0

Methods and Models in Mammary Gland Biology and Breast Cancer Research. 乳腺生物学和乳腺癌研究的方法与模型。

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-03-08 DOI: 10.1007/s10911-025-09579-3

Johanna Englund, Zuzana Sumbalova Koledova

{"title":"Methods and Models in Mammary Gland Biology and Breast Cancer Research.","authors":"Johanna Englund, Zuzana Sumbalova Koledova","doi":"10.1007/s10911-025-09579-3","DOIUrl":"10.1007/s10911-025-09579-3","url":null,"abstract":"The collection on Methods and Models in Mammary Gland Biology and Breast Cancer Research showcases recent advances in tools and models that enhance our understanding of mammary gland development and breast cancer. This collection includes sixteen articles, collectively addressing approaches to investigate key aspects of mammary gland biology and tumorigenesis, including hormonal signaling, tissue architecture, tumor microenvironment, and species-specific mammary development. The issue highlights innovations such as optimized progesterone receptor reporters, improved menopause models, and 3D-printed mammary epithelial structures. It also features advancements in organoid-based studies, in situ labeling of epithelial proliferation in large animals, preclinical models for breast cancer prevention, and high-resolution imaging techniques. Methodologies for studying macrophage-cancer cell interactions and lysosomal function are provided as step-by-step protocols. Additionally, review articles provide insights into diverse mammalian organoid systems, rat mammary tumor models, and strategies for modeling breast cancer metastasis. Together, these contributions advance mammary gland research by refining experimental approaches, expanding model diversity, and fostering translational applications in breast cancer.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"4"},"PeriodicalIF":3.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammalian Species-Specific Resistance to Mammary Cancer. 哺乳动物对乳腺癌的特异性抗性。

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-03-06 DOI: 10.1007/s10911-025-09578-4

Itamar Barash

{"title":"Mammalian Species-Specific Resistance to Mammary Cancer.","authors":"Itamar Barash","doi":"10.1007/s10911-025-09578-4","DOIUrl":"10.1007/s10911-025-09578-4","url":null,"abstract":"Tumorigenesis in mammals is driven by inherited genetic variants, environmental factors and random errors during normal DNA replication that lead to cancer-causing mutations. These factors initiate uncontrolled cellular proliferation and disrupt the regulation of critical checkpoints. A few mammalian species possess unique protective mechanisms that enable them to resist widespread cancer development and achieve longevity. Tissue-specific tumor protection adds another layer of complexity to this diversity. Breast cancer is a leading cause of human mortality, particularly among females. Driven by the need for new strategies in treatment and prevention, this opinion article explores and supports the idea that herbivores are more resistant to mammary cancer than carnivores and omnivores. This diversity has occurred despite the remarkably similar basic mammary biology. Herbivores' meatless diet cannot explain the differences in cancer resistance, which have accompanied species segregation since the Jurassic era. To investigate the causes of this diversity, the characteristics of tumorigenesis in the human breast-and to a lesser extent in other carnivores-have been compared with data from retrospective analyses of bovine mammary tumor development across various locations over the past century. Well-established genomic, cellular, and systemic triggers of breast cancer exhibit different, or less pronounced tissue-specific activity in the bovine mammary gland, accompanied by novel bovine-specific protective mechanisms. Together, these factors contribute to the near absence of breast cancer in bovines and offer a basis for developing future anticancer strategies.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"3"},"PeriodicalIF":3.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recurrent Nipple Duct Obstruction in Two Breastfeeding Patients: A Case Report and Discussion of the Underlying Pathophysiology. 2例母乳喂养患者复发性乳头管阻塞：1例报告及潜在病理生理学的讨论。

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-02-01 DOI: 10.1007/s10911-025-09576-6

Anna Sadovnikova, Susan Greenman, Bridget Young, Casey Rosen-Carole

{"title":"Recurrent Nipple Duct Obstruction in Two Breastfeeding Patients: A Case Report and Discussion of the Underlying Pathophysiology.","authors":"Anna Sadovnikova, Susan Greenman, Bridget Young, Casey Rosen-Carole","doi":"10.1007/s10911-025-09576-6","DOIUrl":"10.1007/s10911-025-09576-6","url":null,"abstract":"Nipple pain is a common reason for premature breastfeeding cessation. There exists anecdotal evidence that one cause of lactational nipple pain is a ductal obstruction, but there is no published literature describing this phenomenon. Herein we present two case reports for two patients who experienced breast and nipple pain concurrent with milk flow reduction. Both patients removed a small stone-like obstructing object from their nipple; this action was painful for one of the patients, resulting in immediate release of milk and relief from breast pain. Both patients experienced recurrence of stone formation in their nipple ducts. We analyzed the mineral composition of the obstructing objects and breast milk using inductively coupled mass spectroscopy. We use literature on teat obstructions in dairy cows and dacryolith and sialolith formation to propose hypotheses as to how the formation of obstructing objects in milk ducts might occur. Future research directions for determining the pathophysiology, clinical presentation, and management of human nipple duct obstructions are discussed.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"2"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defective Mammary Epithelial Outgrowth in Transgenic EKAREV-NLS Mice: Correction via Estrogen Supplementation and Genetic Background Modification. 转基因EKAREV-NLS小鼠乳腺上皮生长缺陷：通过补充雌激素和基因背景修饰进行纠正。

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2025-01-18 DOI: 10.1007/s10911-025-09574-8

Matea Brezak, Zuzana Sumbalova Koledova

{"title":"Defective Mammary Epithelial Outgrowth in Transgenic EKAREV-NLS Mice: Correction via Estrogen Supplementation and Genetic Background Modification.","authors":"Matea Brezak, Zuzana Sumbalova Koledova","doi":"10.1007/s10911-025-09574-8","DOIUrl":"10.1007/s10911-025-09574-8","url":null,"abstract":"Fluorescent biosensors offer a powerful tool for tracking and quantifying protein activity in living systems with high temporospatial resolution. However, the expression of genetically encoded fluorescent proteins can interfere with endogenous signaling pathways, potentially leading to developmental and physiological abnormalities. The EKAREV-NLS mouse model, which carries a FRET-based biosensor for monitoring extracellular signal-regulated kinase (ERK) activity, has been widely utilized both in vivo and in vitro across various cell types and organs. In this study, we report a significant defect in mammary epithelial development in EKAREV-NLS C57BL/6J female mice. Our findings reveal that these mice exhibit severely impaired mammary epithelial outgrowth, linked to systemic defects including disrupted estrous cycling, impaired ovarian follicle maturation, anovulation, and reduced reproductive fitness. Notably, estrogen supplementation was sufficient to enhance mammary epithelial growth in the EKAREV-NLS C57BL/6J females. Furthermore, outcrossing to the ICR genetic background fully restored normal mammary epithelial outgrowth, indicating that the observed phenotype is dependent on genetic background. We also confirmed the functional performance of the biosensor in hormone-supplemented and outcrossed tissues through time-lapse imaging of primary mammary epithelial cells. Our results underscore the critical need for thorough characterization of biosensor-carrying models before their application in specific research contexts. Additionally, this work highlights the influence of hormonal and genetic factors on mammary gland development and emphasizes the importance of careful consideration when selecting biosensor strains for mammary studies.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"30 1","pages":"1"},"PeriodicalIF":3.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription Impairment of TMEM208 by ZBTB14 Suppresses Breast cancer Radiotherapy Resistance. ZBTB14介导的TMEM208转录损伤抑制乳腺癌放疗抵抗

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-12-18 DOI: 10.1007/s10911-024-09573-1

Yan Li, Lili Liang

{"title":"Transcription Impairment of TMEM208 by ZBTB14 Suppresses Breast cancer Radiotherapy Resistance.","authors":"Yan Li, Lili Liang","doi":"10.1007/s10911-024-09573-1","DOIUrl":"10.1007/s10911-024-09573-1","url":null,"abstract":"Zinc finger and BTB domain-containing protein (ZBTB) proteins have been implicated in different cellular processes, including DNA damage responses and cell cycle progression. However, the mechanism by which ZBTB14 modulates radiotherapy (RT) radioresistance (RT-R) remains to be elucidated. We aimed to elucidate the regulation mechanism of ZBTB14 in breast cancer (BC) RT-R. Using integrated bioinformatics prediction, ZBTB14 was identified as a hub transcription factor related to RT-R in BC. ZBTB14 was significantly under-expressed in non-responders and RT-R/BC cells, whereas its target transmembrane protein 208 (TMEM208) was significantly overexpressed in non-responders and RT-R/BC cells. Chromatin immunoprecipitation-qPCR and luciferase reporter assays revealed that ZBTB14 downregulated TMEM208 expression through transcriptional repression. Overexpression of ZBTB14 significantly inhibited the malignant biological behavior of BC cells and tumor growth in vivo, and further upregulation of TMEM208 reversed the biological activity and radiotherapy resistance of RT-R/BC cells inhibited by overexpression of ZBTB14.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"29 1","pages":"20"},"PeriodicalIF":3.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

15th Annual ENBDC Meeting: How do Cellular Potency, Microenvironment and Natural Rhythms Influence Mammary Gland Biology and Breast Cancer? 第 15 届 ENBDC 年会：细胞潜能、微环境和自然节律如何影响乳腺生物学和乳腺癌？

IF 3 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-11-25 DOI: 10.1007/s10911-024-09572-2

Maria Rafaeva, Beata Kaczyńska, Jaime Redondo-Pedraza, Mohamed Bentires-Alj, Walid T Khaled, Silvia Fre

引用次数: 0