MaGNet: A Network-Based Method for Quantitative Analysis of the Mammary Ductal Tree in Developing Female Mice.

IF 3.6 4区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Steven M Lewis, Lucia Tellez-Perez, Samantha Henry, Xingyu Zheng, Saket Navlakha, Camila O Dos Santos
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引用次数: 0

Abstract

The mammary gland is a uniquely dynamic organ with a branching architecture that develops entirely after birth in response to hormonal cues. A common approach in mammary gland biology is the evaluation of branching morphogenesis to characterize the role of developmental, physiological and molecular perturbations on branching tissue invasion, growth, and maintenance. Yet, the field still lacks a fully open-sourced, quantitative framework to analyze whole-mount mammary tissue images, as a commonly utilized methodology. Here, we present MaGNet (Mammary Gland Network analysis tool), a method that leverages network theory to characterize key features of ductal branching during mammary gland development. Applying this pipeline to mammary gland images captured at three pubertal timepoints, we achieved reproducible quantification of ductal tree expansion across development. In addition, this network analysis pipeline captures ductal expansion induced by pregnancy hormones. By providing open-source tools to the research community, this method may increase reproducibility and broad applicability across diverse organ systems, model organisms, and developmental stages.

磁铁:一种基于网络的方法定量分析发育中的雌性小鼠乳腺导管树。
乳腺是一个独特的动态器官,具有分支结构,在出生后完全发育,以响应激素的提示。乳腺生物学中常用的方法是评估分支形态发生,以表征发育、生理和分子扰动对分支组织侵袭、生长和维持的作用。然而,该领域仍然缺乏一个完全开源的定量框架来分析整个乳腺组织图像,作为一种常用的方法。在这里,我们提出了MaGNet(乳腺网络分析工具),这是一种利用网络理论来表征乳腺发育过程中导管分支的关键特征的方法。将该管道应用于三个青春期时间点捕获的乳腺图像,我们实现了整个发育过程中导管树扩张的可重复量化。此外,该网络分析管道捕获了妊娠激素引起的导管扩张。通过向研究界提供开源工具,该方法可以提高可重复性和广泛的适用性,适用于不同的器官系统、模式生物和发育阶段。
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来源期刊
Journal of Mammary Gland Biology and Neoplasia
Journal of Mammary Gland Biology and Neoplasia 医学-内分泌学与代谢
CiteScore
5.30
自引率
4.00%
发文量
22
期刊介绍: Journal of Mammary Gland Biology and Neoplasia is the leading Journal in the field of mammary gland biology that provides researchers within and outside the field of mammary gland biology with an integrated source of information pertaining to the development, function, and pathology of the mammary gland and its function. Commencing in 2015, the Journal will begin receiving and publishing a combination of reviews and original, peer-reviewed research. The Journal covers all topics related to the field of mammary gland biology, including mammary development, breast cancer biology, lactation, and milk composition and quality. The environmental, endocrine, nutritional, and molecular factors regulating these processes is covered, including from a comparative biology perspective.
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