Journal of Mammary Gland Biology and Neoplasia最新文献_第2页

The Lymphatic System in Mammary Gland Biology and Breast Cancer. 乳腺生物学和乳腺癌中的淋巴系统。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-03-17 DOI: 10.1007/s10911-024-09558-0

Traci R Lyons, Esta Sterneck

引用次数: 0

Spatial Engineering of Mammary Epithelial Cell Cultures with 3D Bioprinting Reveals Growth Control by Branch Point Proximity. 三维生物打印乳腺上皮细胞培养的空间工程揭示了分支点邻近对生长的控制。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-02-28 DOI: 10.1007/s10911-024-09557-1

Leena M Koskinen, Lari Nieminen, Antti Arjonen, Camilo Guzmán, Markus Peurla, Emilia Peuhu

{"title":"Spatial Engineering of Mammary Epithelial Cell Cultures with 3D Bioprinting Reveals Growth Control by Branch Point Proximity.","authors":"Leena M Koskinen, Lari Nieminen, Antti Arjonen, Camilo Guzmán, Markus Peurla, Emilia Peuhu","doi":"10.1007/s10911-024-09557-1","DOIUrl":"10.1007/s10911-024-09557-1","url":null,"abstract":"The three-dimensional (3D) structure of the ductal epithelium and the surrounding extracellular matrix (ECM) are integral aspects of the breast tissue, and they have important roles during mammary gland development, function and malignancy. However, the architecture of the branched mammary epithelial network is poorly recapitulated in the current in vitro models. 3D bioprinting is an emerging approach to improve tissue-mimicry in cell culture. Here, we developed and optimized a protocol for 3D bioprinting of normal and cancerous mammary epithelial cells into a branched Y-shape to study the role of cell positioning in the regulation of cell proliferation and invasion. Non-cancerous cells formed continuous 3D cell networks with several organotypic features, whereas the ductal carcinoma in situ (DCIS) -like cancer cells exhibited aberrant basal polarization and defective formation of the basement membrane (BM). Quantitative analysis over time demonstrated that both normal and cancerous cells proliferate more at the branch tips compared to the trunk region of the 3D-bioprinted cultures, and particularly at the tip further away from the branch point. The location-specific rate of proliferation was independent of TGFβ signaling but invasion of the DCIS-like breast cancer cells was reduced upon the inhibition of TGFβ. Thus, our data demonstrate that the 3D-bioprinted cells can sense their position in the branched network of cells and proliferate at the tips, thus recapitulating this feature of mammary epithelial branching morphogenesis. In all, our results demonstrate the capacity of the developed 3D bioprinting method for quantitative analysis of the relationships between tissue structure and cell behavior in breast morphogenesis and cancer.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protocols for Co-Culture Phenotypic Assays with Breast Cancer Cells and THP-1-Derived Macrophages. 乳腺癌细胞与 THP-1 衍生巨噬细胞共培养表型试验规程

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-02-10 DOI: 10.1007/s10911-024-09556-2

Alicja Mazan, Anna A Marusiak

{"title":"Protocols for Co-Culture Phenotypic Assays with Breast Cancer Cells and THP-1-Derived Macrophages.","authors":"Alicja Mazan, Anna A Marusiak","doi":"10.1007/s10911-024-09556-2","DOIUrl":"10.1007/s10911-024-09556-2","url":null,"abstract":"Tumor mass comprises not only cancer cells but also heterogeneous populations of immune and stromal cells, along with the components of the extracellular matrix, collectively called the tumor microenvironment (TME). This diverse population of cells can communicate with each other, which can positively or negatively affect tumor growth and progression to malignancy. The most common type of immune cells in the TME are macrophages. Macrophages continuously differentiate into a broad landscape of tumor-associated macrophages (TAMs) in response to numerous signals from the TME, which makes studies on TAMs quite challenging. Therefore, implementing reliable protocols is a milestone for drawing consistent conclusions about the interactions between cancer cells and TAMs. Here, we provide the details for the polarization of a human leukemia monocytic cell line, THP-1, into M0, M1 and M2 macrophages. We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture with macrophages to measure the influence of macrophages on the oncogenic features of cancer cells. In summary, our co-culture-based protocols can be a valuable resource for investigating the interactions between macrophages and cancer cells.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast Ultrasound Scanning is a Rapid, Sensitive, Precise and Cost-Effective Method to Monitor Tumor Grafts in Mice. 快速超声扫描是一种快速、灵敏、精确且经济高效的小鼠肿瘤移植物监测方法。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-01-30 DOI: 10.1007/s10911-024-09555-3

Sébastien Molière, Arthur Martinet, Amélie Jaulin, Massimo Lodi, Thien-Nga Chamaraux-Tran, Fabien Alpy, Guillaume Bierry, Catherine Tomasetto

{"title":"Fast Ultrasound Scanning is a Rapid, Sensitive, Precise and Cost-Effective Method to Monitor Tumor Grafts in Mice.","authors":"Sébastien Molière, Arthur Martinet, Amélie Jaulin, Massimo Lodi, Thien-Nga Chamaraux-Tran, Fabien Alpy, Guillaume Bierry, Catherine Tomasetto","doi":"10.1007/s10911-024-09555-3","DOIUrl":"10.1007/s10911-024-09555-3","url":null,"abstract":"In preclinical studies, accurate monitoring of tumor dynamics is crucial for understanding cancer biology and evaluating therapeutic interventions. Traditional methods like caliper measurements and bioluminescence imaging (BLI) have limitations, prompting the need for improved imaging techniques. This study introduces a fast-scan high-frequency ultrasound (HFUS) protocol for the longitudinal assessment of syngeneic breast tumor grafts in mice, comparing its performance with caliper, BLI measurements and with histological analysis. The E0771 mammary gland tumor cell line, engineered to express luciferase, was orthotopically grafted into immunocompetent C57BL/6 mice. Tumor growth was monitored longitudinally at multiple timepoints using caliper measurement, HFUS, and BLI, with the latter two modalities assessed against histopathological standards post-euthanasia. The HFUS protocol was designed for rapid, anesthesia-free scanning, focusing on volume estimation, echogenicity, and necrosis visualization. All mice developed tumors, only 20.6% were palpable at day 4. HFUS detected tumors as small as 2.2 mm in average diameter from day 4 post-implantation, with an average scanning duration of 47 s per mouse. It provided a more accurate volume assessment than caliper, with a lower average bias relative to reference tumor volume. HFUS also revealed tumor necrosis, correlating strongly with BLI in terms of tumor volume and cellularity. Notable discrepancies between HFUS and BLI growth rates were attributed to immune cell infiltration. The fast HFUS protocol enables precise and efficient tumor assessment in preclinical studies, offering significant advantages over traditional methods in terms of speed, accuracy, and animal welfare, aligning with the 3R principle in animal research.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10827948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones. 小鼠和人类乳腺器官组织对雌性激素反应的单细胞转录图谱

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-01-30 DOI: 10.1007/s10911-023-09553-x

Jenelys Ruiz Ortiz, Steven M Lewis, Michael Ciccone, Deeptiman Chatterjee, Samantha Henry, Adam Siepel, Camila O Dos Santos

引用次数: 0

Toward Characterizing Lymphatic Vasculature in the Mammary Gland During Normal Development and Tumor-Associated Remodeling 了解正常发育和肿瘤相关重塑过程中乳腺淋巴管的特征

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2024-01-13 DOI: 10.1007/s10911-023-09554-w

Petra Dahms, T. Lyons

引用次数: 0

Single-cell Analysis Reveals Inter- and Intratumour Heterogeneity in Metastatic Breast Cancer 单细胞分析揭示转移性乳腺癌的瘤间和瘤内异质性

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2023-12-08 DOI: 10.1007/s10911-023-09551-z

Baptiste Hamelin, Milan M. S. Obradović, Atul Sethi, Michal Kloc, Simone Münst, Christian Beisel, Katja Eschbach, Hubertus Kohler, Savas Soysal, Marcus Vetter, Walter P. Weber, Michael B. Stadler, Mohamed Bentires-Alj

{"title":"Single-cell Analysis Reveals Inter- and Intratumour Heterogeneity in Metastatic Breast Cancer","authors":"Baptiste Hamelin, Milan M. S. Obradović, Atul Sethi, Michal Kloc, Simone Münst, Christian Beisel, Katja Eschbach, Hubertus Kohler, Savas Soysal, Marcus Vetter, Walter P. Weber, Michael B. Stadler, Mohamed Bentires-Alj","doi":"10.1007/s10911-023-09551-z","DOIUrl":"https://doi.org/10.1007/s10911-023-09551-z","url":null,"abstract":"Metastasis is the leading cause of cancer-related deaths of breast cancer patients. Some cancer cells in a tumour go through successive steps, referred to as the metastatic cascade, and give rise to metastases at a distant site. We know that the plasticity and heterogeneity of cancer cells play critical roles in metastasis but the precise underlying molecular mechanisms remain elusive. Here we aimed to identify molecular mechanisms of metastasis during colonization, one of the most important yet poorly understood steps of the cascade. We performed single-cell RNA-Seq (scRNA-Seq) on tumours and matched lung macrometastases of patient-derived xenografts of breast cancer. After correcting for confounding factors such as the cell cycle and the percentage of detected genes (PDG), we identified cells in three states in both tumours and metastases. Gene-set enrichment analysis revealed biological processes specific to proliferation and invasion in two states. Our findings suggest that these states are a balance between epithelial-to-mesenchymal (EMT) and mesenchymal-to-epithelial transitions (MET) traits that results in so-called partial EMT phenotypes. Analysis of the top differentially expressed genes (DEGs) between these cell states revealed a common set of partial EMT transcription factors (TFs) controlling gene expression, including ZNF750, OVOL2, TP63, TFAP2C and HEY2. Our data suggest that the TFs related to EMT delineate different cell states in tumours and metastases. The results highlight the marked interpatient heterogeneity of breast cancer but identify common features of single cells from five models of metastatic breast cancer.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties. TGFß1刺激淋巴内皮细胞产生IL7和IL15，它们是具有间质特性的乳腺癌细胞的趋化因子。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2023-12-06 DOI: 10.1007/s10911-023-09552-y

Nikolina Giotopoulou, Wenyang Shi, Malgorzata Maria Parniewska, Wenwen Sun, Jonas Fuxe

{"title":"TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties.","authors":"Nikolina Giotopoulou, Wenyang Shi, Malgorzata Maria Parniewska, Wenwen Sun, Jonas Fuxe","doi":"10.1007/s10911-023-09552-y","DOIUrl":"10.1007/s10911-023-09552-y","url":null,"abstract":"The lymphatic system is a major gateway for tumor cell dissemination but the mechanisms of how tumor cells gain access to lymphatic vessels are not completely understood. Breast cancer cells undergoing epithelial-mesenchymal transition (EMT) gain invasive and migratory properties. Overexpression of the cytokine transforming growth factor β1 (TGFβ1), a potent inducer of EMT, is frequently detected in the tumor microenvironment and correlates with invasion and lymph metastasis. Recently, we reported that TGFβ1 stimulated breast cancer cells with mesenchymal properties to migrate in a targeted fashion towards the lymphatic system via CCR7/CCL21-mediated chemotaxis, similar to dendritic cells during inflammation. Here, we aimed to identify additional chemotactic factors and corresponding receptors that could be involved in guiding breast cancer cells through the lymphatic system. Through a combination of RNA sequencing analysis, database screening and invasion assays we identified IL7/IL7R and IL15/IL15R as pairs of chemokines and receptors with potential roles in promoting chemotactic migration of breast cancer cells with mesenchymal properties towards the lymphatics. The results demonstrate the capacity of TGFβ1 to orchestrate crosstalk between tumor cells and lymphatic endothelial cells and warrant further studies to explore the roles of IL7 and IL15 in promoting lymph metastasis of breast cancer.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10700205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A molecular toolbox to study progesterone receptor signaling. 研究孕激素受体信号的分子工具箱。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2023-11-29 DOI: 10.1007/s10911-023-09550-0

Marleen T Aarts, Muriel Wagner, Tanne van der Wal, Antonius L van Boxtel, Renée van Amerongen

{"title":"A molecular toolbox to study progesterone receptor signaling.","authors":"Marleen T Aarts, Muriel Wagner, Tanne van der Wal, Antonius L van Boxtel, Renée van Amerongen","doi":"10.1007/s10911-023-09550-0","DOIUrl":"10.1007/s10911-023-09550-0","url":null,"abstract":"Progesterone receptor (PR) signaling is required for mammary gland development and homeostasis. A major bottleneck in studying PR signaling is the lack of sensitive assays to measure and visualize PR pathway activity both quantitatively and spatially. Here, we develop new tools to study PR signaling in human breast epithelial cells. First, we generate optimized Progesterone Responsive Element (PRE)-luciferase constructs and demonstrate that these new reporters are a powerful tool to quantify PR signaling activity across a wide range of progesterone concentrations in two luminal breast cancer cell lines, MCF7 and T47D. We also describe a fluorescent lentiviral PRE-GFP reporter as a novel tool to visualize PR signaling at the single-cell level. Our reporter constructs are sensitive to physiological levels of progesterone. Second, we show that low background signaling, and high levels of PR expression are a prerequisite for robustly measuring PR signaling. Increasing PR expression by transient transfection, stable overexpression in MCF7 or clonal selection in T47D, drastically improves both the dynamic range of luciferase reporter assays, and the induction of endogenous PR target genes as measured by qRT-PCR. We find that the PR signaling response differs per cell line, target gene and hormone concentration used. Taken together, our tools allow a more rationally designed approach for measuring PR signaling in breast epithelial cells.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fourteenth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer. 第十四届ENBDC年度研讨会：乳腺生物学和癌症方法。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2023-10-06 DOI: 10.1007/s10911-023-09549-7

Silke Blair Chalmers, Tanne van der Wal, Silvia Fre, Jos Jonkers

引用次数: 0