乳腺导管内注射腺病毒进行谱系追踪。

IF 3.6 4区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Xueqing Chen, Sen Han, Dongyi Zhao, Zhe Li
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引用次数: 0

摘要

谱系追踪是发育生物学和癌症研究的基本工具,为细胞命运决定、组织稳态和肿瘤发生提供了重要的见解。乳腺是一个高度动态的器官,具有复杂的细胞层次结构,使其成为谱系追踪研究的理想系统。经典的方法,如他莫昔芬诱导的CreER/loxP重组,极大地提高了我们对乳腺上皮细胞(MEC)分化、稳态和转化的理解。然而,这些方法存在局限性,包括他莫昔芬对雌激素信号的潜在影响,乳腺特异性较低,以及需要转基因模型和小鼠育种。基于腺病毒cre (Ad-Cre)的谱系追踪已成为一种强大的替代方法,可实现快速和器官特异性重组。本文综述了Ad-Cre方法在乳腺生物学中的综合评价,比较了其效率、特异性和基于creer方法的技术优势。我们讨论了基于导管内注射的Ad-Cre谱系追踪在MEC命运定位、乳腺癌细胞起源识别和肿瘤进展建模中的应用。此外,我们强调其在克隆水平上诱导遗传标记的能力,有助于精确研究MEC的可塑性和肿瘤细胞的异质性。尽管具有优势,但Ad-Cre谱系追踪也存在挑战,如细胞靶向效率低和对乳腺免疫微环境的潜在影响。未来的进展,包括基于crispr的条形码的整合,可能会进一步增强其在高分辨率命运图谱中的实用性。通过总结近年来的进展和比较分析,本文强调了Ad-Cre谱系追踪作为一种多功能和强大的工具在乳腺生物学和乳腺癌研究中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Intraductal Injection of Adenoviruses to Perform Lineage Tracing in the Mammary Gland.

Intraductal Injection of Adenoviruses to Perform Lineage Tracing in the Mammary Gland.

Lineage tracing is a fundamental tool in developmental biology and cancer research, providing critical insights into cell fate decisions, tissue homeostasis and tumor initiation. The mammary gland is a highly dynamic organ with a complex cellular hierarchy, making it an ideal system for lineage-tracing studies. Classic approaches, such as tamoxifen-inducible CreER/loxP recombination, have significantly advanced our understanding of mammary epithelial cell (MEC) differentiation, homeostasis, and transformation. However, these methods have limitations, including potential effects of tamoxifen on estrogen signaling, low mammary gland specificity, and the requirement for transgenic model creation and mouse breeding. Adenovirus-Cre (Ad-Cre)-based lineage tracing has emerged as a powerful alternative, enabling rapid and organ-specific recombination. This review provides a comprehensive evaluation of the Ad-Cre approach in mammary gland biology, comparing its efficiency, specificity, and technical advantages over the CreER-based method. We discuss applications of Ad-Cre intraductal injection-based lineage tracing in mapping MEC fates, identifying the cellular origins of breast cancer, and modeling tumor progression. Additionally, we highlight its ability to induce genetic marking at a clonal level, facilitating precise investigations into MEC plasticity and tumor cell heterogeneity. Despite its advantages, Ad-Cre lineage tracing also presents challenges, such as low cell-targeting efficiency and potential effect on the mammary gland immune microenvironment. Future advancements, including the integration of CRISPR-based barcoding, may further enhance its utility for high-resolution fate mapping. By summarizing recent advancements and comparative analyses, this review underscores the significance of Ad-Cre lineage tracing as a versatile and powerful tool in mammary gland biology and breast cancer research.

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来源期刊
Journal of Mammary Gland Biology and Neoplasia
Journal of Mammary Gland Biology and Neoplasia 医学-内分泌学与代谢
CiteScore
5.30
自引率
4.00%
发文量
22
期刊介绍: Journal of Mammary Gland Biology and Neoplasia is the leading Journal in the field of mammary gland biology that provides researchers within and outside the field of mammary gland biology with an integrated source of information pertaining to the development, function, and pathology of the mammary gland and its function. Commencing in 2015, the Journal will begin receiving and publishing a combination of reviews and original, peer-reviewed research. The Journal covers all topics related to the field of mammary gland biology, including mammary development, breast cancer biology, lactation, and milk composition and quality. The environmental, endocrine, nutritional, and molecular factors regulating these processes is covered, including from a comparative biology perspective.
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