Journal of invertebrate pathology最新文献

{"title":"Microsporidia-cypovirus interactions during simultaneous infection of the tree defoliator Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)","authors":"Arina S. Rumiantseva ,&nbsp;Aleksander A. Ageev ,&nbsp;Anastasia N. Ignatieva ,&nbsp;Maria E. Yakimova ,&nbsp;Daria D. Kharlamova ,&nbsp;Vyacheslav V. Martemyanov ,&nbsp;Yuri S. Tokarev","doi":"10.1016/j.jip.2024.108199","DOIUrl":"10.1016/j.jip.2024.108199","url":null,"abstract":"<div><p>The Siberian moth, <em>Dendrolimus sibiricus</em> is a dangerous forest defoliator, the number one pest of boreal forests in Asia. Search for effective and ecologically friendly control measures drives attention to microbial pathogens. Viruses and microsporidia are obligate intracellular parasites widespread in insect populations causing either chronic or acute infections. Interactions of these pathogens vary from antagonistic to synergistic. The goal of the work was to test a recently discovered cytoplasmatic polyhedrosis virus (cypovirus) strain DsCPV-1 isolated from <em>D.sibiricus</em>, combined with a microsporidium, against <em>D. sibiricus,</em> by feeding the inoculum (viral polyhedral and microsporidian spores)<em>.</em> Three different microsporidian parasites of lepidopterans were tested against <em>D. sibiricus</em> as monoinfection: <em>Nosema bombycis</em> from silkworm<em>, N. pyrausta</em> from corn borer<em>,</em> and <em>Tubulinosema loxostegi</em> from beet webworm. <em>Nosema bombycis</em> was the most virulent, with a median lethal time of 7 days in the first and second instars treated with 100,000 and 1 million spores/larva, respectively. <em>Nosema bombycis</em> (dose 100,000 spores/larva) was chosen to test it as mixed infection in combination with an extremely low dose of DsCPV-1 (1 polyhedron/larva) against two races of <em>D. sibiricus</em> second instar larvae (the fir-feeding race and the larch-feeding race). The mixed infection demonstrated the most prominent negative effect on larval lethal time and weight for the both tested races. Mixed infections showed a synergistic effect for the fir-feeding larvae but additive effect only for the larch feeding larvae. Both pathogens co-developed successfully in the larvae with equal ratio of producing inoculum. The combination of these entomopathogens is therefore promising for forest protection against the Siberian moth and could be the way to significantly decrease the amount of pathogens applied in field.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108199"},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomopathogenic nematode detection and counting model developed based on A-star algorithm","authors":"Hilal Erdoğan","doi":"10.1016/j.jip.2024.108196","DOIUrl":"10.1016/j.jip.2024.108196","url":null,"abstract":"<div><p>Entomopathogenic nematodes are soil-dwelling living organisms widely employed in the biological control of agricultural insect pests, serving as a significant alternative to pesticides. In laboratory procedures, the counting process remains the most common, labor-intensive, time-consuming, and approximate aspect of studies related to entomopathogenic nematodes. In this context, a novel method has been proposed for the detection and quantification of <em>Steinernema feltiae</em> isolate using computer vision on microscope images. The proposed method involves two primary algorithmic steps: framing and isolation. Compared to YOLO-V5m, YOLO-V7m, and YOLO-V8m, the A-<em>star</em>-based developed network demonstrates significantly improved detection accuracy compared to other networks. The novel method is particularly effective in facilitating the detection of overlapping nematodes. The developed algorithm excludes processes that increase space and time complexity, such as the weight document, which contains the learned parameters of the deep learning model, model integration, and prediction time, resulting in more efficient operation. The results indicate the feasibility of the proposed method for detecting and counting entomopathogenic nematodes.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108196"},"PeriodicalIF":3.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic di-GMP incorporates the transcriptional factor FleQ03 in Pseudomonas syringae MB03 to elicit biofilm-dependent resistance in response to Caenorhabditis elegans predation","authors":"Xiaowen Sun ,&nbsp;Fan Xiao ,&nbsp;Yuwei Su ,&nbsp;Zhi Li ,&nbsp;Xun Yu ,&nbsp;Rebecca E. Parales ,&nbsp;Lin Li","doi":"10.1016/j.jip.2024.108189","DOIUrl":"10.1016/j.jip.2024.108189","url":null,"abstract":"<div><p>Bacteria usually form biofilms as a defense mechanism against predation by bacterivorous nematodes. In this context, the second messenger c-di-GMP from the wild-type <em>Pseudomonas syringae</em> MB03 actuates the transcriptional factor FleQ03 to elicit biofilm-dependent nematicidal activity against <em>Caenorhabditis elegans</em> N2. <em>P. syringae</em> MB03 cells exhibited nematicidal activity and c-di-GMP content in <em>P. syringae</em> MB03 cells was increased after feeding to nematodes. Expression of a diguanylate cyclase (DGC) gene in <em>P. syringae</em> MB03 resulted in an increased c-di-GMP content, biofilm yield and nematicidal activity, whereas converse effects were obtained when expressing a phosphodiesterase (PDE) gene. Molecular docking and isothermal titration calorimetry assays verified the affinity activity between c-di-GMP and the FleQ03 protein. The disruption of the <em>fleQ03</em> gene in <em>P. syringae</em> MB03, while increasing c-di-GMP content, significantly diminished both biofilm formation and nematicidal activity. Interestingly, <em>P. syringae</em> MB03 formed a full-body biofilm around the worms against predation, probably extending from the tail to the head, whereas it was not observed in the <em>fleQ03</em> gene disrupted cells. Thus, we hypothesized that c-di-GMP incorporated FleQ03 to reinforce bacterial biofilm and biofilm-dependent pathogenicity in response to <em>C. elegans</em> predation, providing insights into a possible means of resisting bacterivorous nematodes by bacteria in natural ecosystems.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108189"},"PeriodicalIF":3.6,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deleterious effects of Wolbachia on life history and physiological traits of common pill woodlice","authors":"Charlotte Depeux ,&nbsp;Angèle Branger ,&nbsp;Hélène Paulhac,&nbsp;Romain Pigeault,&nbsp;Sophie Beltran-Bech","doi":"10.1016/j.jip.2024.108187","DOIUrl":"10.1016/j.jip.2024.108187","url":null,"abstract":"<div><p>Most of eukaryotic organisms live in close interaction with micro-organisms called symbionts. Symbiotic interactions underpin the evolution of biological complexity, the health of organisms and, ultimately, the proper functioning of ecosystems. While some symbionts confer adaptive benefits on their host (mutualistic symbionts) and others clearly induce costs (parasitic symbionts), a number of micro-organisms are difficult to classify because they have been described as conferring both benefits and costs on their host. This is particularly true of the most widespread animal endosymbiont, <em>Wolbachia pipientis</em>. In this study, we investigated the influence of <em>Wolbachia</em> infection on a broad spectrum of ecological and physiological parameters of one of its native hosts, <em>Armadillidium vulgare</em>. The aim was to gain as complete a picture as possible of the influence of this endosymbiont on its host. Our results showed that the presence of <em>Wolbachia</em> resulted in a decrease in individual reproductive success and survival. Host immune cells density decreased and β-galactosidase activity (ageing biomarker) increased with the presence of <em>Wolbachia</em>, suggesting a negative impact of this endosymbiont on woodlice health. While previous studies have shown that <em>Wolbachia</em> can have a positive impact on the immunocompetence of <em>A. vulgare</em>, here we shed more light on the costs of infection. Our results illustrate the complex dynamics that exist between <em>Wolbachia</em> and its arthropod host and therefore offer valuable insights into the intricate interplay of symbiotic relationships in ecological systems.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108187"},"PeriodicalIF":3.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022201124001307/pdfft?md5=888d77f6e403b853ce1715de122adcd0&pid=1-s2.0-S0022201124001307-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bombyx mori serpin 3 is involved in innate immunity by interacting with serine protease 7 to regulate prophenoloxidase activation","authors":"Hui-Hua Cao ,&nbsp;Yu-Ling Wang ,&nbsp;Shahzad Toufeeq ,&nbsp;Wei-Wei Kong ,&nbsp;Sadaf Ayaz ,&nbsp;Shi-Huo Liu ,&nbsp;Jie Wang ,&nbsp;Jia-Ping Xu","doi":"10.1016/j.jip.2024.108188","DOIUrl":"10.1016/j.jip.2024.108188","url":null,"abstract":"<div><p>A subfamily of conserved proteins called serpins plays crucial roles in various physiological functions, particularly in the activation pathway of the serine protease cascade, an essential component of insect innate immunity. Here, we found <em>Bombyx mori serpin 3</em> (<em>BmSerpin3</em>) was most highly expressed in the fat body, and was up-regulated after exposure to bacteria, fungus and virus. Further, the expression of <em>BmSerpin3</em> in the hemocytes, fat body, midgut of silkworm larvae, and BmN cells was up-regulated upon Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Through Bac-to-Bac expression system, we obtained the active protein of BmSerpin3, and the enzyme activity assay showed that BmSerpin3 significantly inhibited the activity of both subtilisin and trypsin. In addition, BmSerpin3 could inhibit the activation of prophenoloxidase (PPO) in larvae. The knockdown of <em>BmSerpin3</em> showed increased phenoloxidase (PO) activity compared to control after BmNPV infection. Ultimately, we confirmed that BmSerpin3 interacts with <em>B. mori</em> Serine Protease 7 (BmSP7). Hence, we hypothesize that BmSerpin3 is involved in innate immunity by interacting with BmSP7 to regulate the PPO activation cascade. Taken together, these results showed that BmSerpin3 play a role in silkworm innate immunity and lay a foundation for studying its functions.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108188"},"PeriodicalIF":3.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiont-mediated antisense RNA delivery controls Nosema ceranae infections in Apis mellifera","authors":"Sihan Wang ,&nbsp;Haoyang Li ,&nbsp;Ruyi Shi ,&nbsp;Yuqi Fu","doi":"10.1016/j.jip.2024.108185","DOIUrl":"10.1016/j.jip.2024.108185","url":null,"abstract":"<div><p><em>Nosema ceranae</em> is a main parasite for honeybees (<em>Apis mellifera</em>) which causes colony collapse in spring. Effective management of <em>N. ceranae</em> infections in bees is imperative for beekeepers. RNA interference (RNAi) has been proven a promising method to control bee pathogens, including IAPV, <em>Varroa destructor</em>, and <em>Nosema.</em> Most studies in this field focused on oral inoculation of double-stranded RNA (dsRNA). We developed an easier method with long-term RNAi effects by engineering the bee symbiont, <em>Bacillus subtilis</em>, to deliver single-stranded antisense RNA (asRNA) in the bee guts, targeting <em>N. ceranae</em> genes. We interfered with the expression of a spore wall protein (SWP12) and a polar tube protein (PTP3) of <em>N. ceranae</em>, resulting in a 60.5% increase in bee lifespan and a 72.7% decrease in <em>Nosema</em> spore load. Our research introduced a novel approach to bee parasite control: <em>B. subtilis</em>-mediated asRNA delivery. Our strategy simplifies the procedure of RNAi, presenting a more efficient mechanism with both prophylactic and therapeutic effects on <em>N. ceranae</em>-infected bees.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108185"},"PeriodicalIF":3.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive consequences of the interaction Trypanosoma cruzi − Triatoma infestans and its trade-off with survival","authors":"Patricia A. Lobbia ,&nbsp;Claudia Rodríguez ,&nbsp;Carolina Remón ,&nbsp;Mariana Manteca-Acosta","doi":"10.1016/j.jip.2024.108183","DOIUrl":"10.1016/j.jip.2024.108183","url":null,"abstract":"<div><p>Relative little is known about fitness effects and life history trade-off of <em>Trypanosoma cruzi</em> in <em>Triatoma infestans</em>, the main vector of Chagas disease in Argentina. Previous studies revealed some costs related to development, excretion, and toxicology or their possible trade-offs, but none address effects on reproduction. To study the effect of <em>T. cruzi</em> infection on reproductive efficiency and survival of <em>T. infestans</em> we set up four treatments: both genders uninfected, both genders infected, female infected – males uninfected and female uninfected − males infected. The infection was induced during the third, fourth, and fifth nymphal instars. Reproductive efficiency and longevity variables were recorded. Our results showed that the infection by <em>T. cruzi</em> increased reproductive efficiency and reduced survival of <em>T. infestans</em>. Pairs where one or both individuals were infected presented a greater percentage copulation, of egg-laying females, the onset of copulation and oviposition occurred earlier, and age-specific fecundity was notably higher. Regarding fertility, infected females displayed higher rates irrespective of the infective status of the male counterpart. A reduction in longevity was observed in infected males and females. These findings highlighted that the infection significantly alters the trade-off reproductive efficiency-survival of <em>T. infestans</em>, with the impact differing according to the infection status of each gender, suggesting a complex interplay rather than a simple additive effect. This response corresponds to the reproductive compensation hypothesis.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"206 ","pages":"Article 108183"},"PeriodicalIF":3.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “High variation of virulence in Aphanomyces astaci strains lacks association with pathogenic traits and mtDNA haplogroups” [J. Invertebr. Pathol. 206 (2024) 108153]","authors":"Caterina Francesconi ,&nbsp;Ljudevit Luka Boštjančić ,&nbsp;Lena Bonassin ,&nbsp;Leonie Schardt ,&nbsp;Christelle Rutz ,&nbsp;Jenny Makkonen ,&nbsp;Klaus Schwenk ,&nbsp;Odile Lecompte ,&nbsp;Kathrin Theissinger","doi":"10.1016/j.jip.2024.108174","DOIUrl":"10.1016/j.jip.2024.108174","url":null,"abstract":"","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"206 ","pages":"Article 108174"},"PeriodicalIF":3.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022201124001174/pdfft?md5=9a7b6d75d11afdc46f40ceab4ba095ce&pid=1-s2.0-S0022201124001174-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term monitoring of Paenibacillus larvae, causative agent of American Foulbrood, in Uruguay","authors":"Sofía Palacios ,&nbsp;Guillermo Añon ,&nbsp;Daniela Arredondo ,&nbsp;Matilde Alarcón ,&nbsp;Pablo Zunino ,&nbsp;Juan Campá ,&nbsp;Jorge Harriet ,&nbsp;Karina Antúnez","doi":"10.1016/j.jip.2024.108186","DOIUrl":"10.1016/j.jip.2024.108186","url":null,"abstract":"<div><p><em>Paenibacillus larvae</em> is the causative agent of American Foulbrood (AFB), the most severe bacterial disease affecting honey bee <em>(Apis mellifera</em>) larvae. It was first reported in Uruguay in 1999. Here, we summarize the monitoring strategy carried out from 2001 to date, based on nationwide surveys sampling honey from colonies (2001/2002, 2011, 2021) or from honey storage tanks (2014–2019). We also discuss the actions carried out for the prevention of AFB outbreaks. Uruguay’s experience in managing AFB for nearly 25 years without antibiotic use, might provide some helpful ideas for other countries working on AFB control programs.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108186"},"PeriodicalIF":3.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening for White Spot Syndrome Virus (WSSV) in pink shrimp (Penaeus paulensis) from Rocha Lagoon, Uruguay","authors":"Daniela Carnales ,&nbsp;Leonardo Galli ,&nbsp;Elena Fabiano ,&nbsp;Graciela Fabiano ,&nbsp;Jamie Bojko ,&nbsp;Marianoel Pereira-Gómez","doi":"10.1016/j.jip.2024.108184","DOIUrl":"10.1016/j.jip.2024.108184","url":null,"abstract":"<div><p><em>Penaeus paulensis</em> (pink shrimp) is an important resource for small-scale fisheries in the brackish coastal lagoons of Uruguay. No viral diseases have been detected in shrimp populations in the Uruguayan territory. The presence of viral pathogens, such as White Spot Syndrome Virus (WSSV) and Infectious Hypodermal Haematopoietic Necrosis Virus (IHHNV) in wild shrimp populations has been previously reported in Brazil and Argentina.</p><p>We investigated the presence of WSSV in wild populations of penaeid shrimp from Rocha Lagoon, Uruguay. We sampled 70 specimens of juvenile <em>P. paulensis</em> and assessed the presence of these viral pathogens using nested PCR and histology. Gill tissue from the 70 samples was divided into 14 pools of 5 individuals for DNA extraction and PCR analysis. We also retested each pooled sample individually. The nested PCR procedure described in the WOAH aquatic animal manual was used. A subset of 20 individual specimens were also processed using standard histological techniques.</p><p>The results showed that WSSV was not detected in the pooled or individually tested samples. We found no evidence of the presence of the viral genome or gill lesions in the samples analysed. This indicates that the fishery is still likely to be free of WSSV infection. The procedures and information generated can be used as a baseline study for future implementation of surveillance programmes in the country.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"206 ","pages":"Article 108184"},"PeriodicalIF":3.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}