Journal of invertebrate pathology最新文献

{"title":"Effects of insect odor cues and infective juvenile age on the host-seeking behavior of Steinernema siamkayai","authors":"Puping Ta-oun ,&nbsp;Toyoshi Yoshiga","doi":"10.1016/j.jip.2025.108280","DOIUrl":"10.1016/j.jip.2025.108280","url":null,"abstract":"<div><div>Entomopathogenic nematodes (EPNs) are soil-dwelling, insect-parasitic nematodes used in biological control. Infective juveniles (IJs), a third-stage free-living stage, are responsible for foraging and infecting suitable insect hosts, exhibiting cruising, ambushing, or intermediate foraging strategies. <em>Steinernema siamkayai</em>, an ambush forager, is a valuable biological control agent in Thailand, yet little is known about its behavioral response to host cues and insect odors. This study examines the olfactory responses of <em>S. siamkayai</em> IJs to insect odorants, including CO₂, compared to an ambusher, <em>S. carpocapsae</em>, and an intermediate forager, <em>S. monticolum</em>. Using a chemotaxis assay on agar plates, we found <em>S. siamkayai</em> and <em>S. monticolum</em> were attracted to insect odors, but attraction diminished when CO₂ was removed with soda lime, whereas <em>S. carpocapsae</em> showed minimal attraction. <em>S. siamkayai</em> and <em>S. monticolum</em> exhibited a stronger response to CO₂ than <em>S. carpocapsae</em>. Additionally, in a sand column assay assessing vertical movement, all three EPNs responded to insect odors from below, with odor response declining as IJ age increased, indicating age-related changes in host-seeking behavior. These results suggest that although <em>S. siamkayai</em> and <em>S. carpocapsae</em> are ambushers within the same phylogenetic clade, their host detection mechanisms differ.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108280"},"PeriodicalIF":3.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untangling a web of spider fungi: Gibellula agroflorestalis (Hypocreales, Ascomycota), a new species of spider parasite from Brazil","authors":"Julie Erica da Rocha Alves,&nbsp;Ana Carla da Silva Santos,&nbsp;Sheila Karine Belo Pedroso,&nbsp;Roger Fagner Ribeiro Melo,&nbsp;Patricia Vieira Tiago","doi":"10.1016/j.jip.2025.108278","DOIUrl":"10.1016/j.jip.2025.108278","url":null,"abstract":"<div><div><em>Gibellula</em> (Cordycipitaceae) includes widely distributed spider pathogenic fungi. This study describes a novel species, <em>Gibellula agroflorestalis</em>, recorded in agroforestry systems (AFS) and Atlantic Forest remnants in Pernambuco State, northeastern Brazil. The morphology of synnemata and conidiophores was studied, and phylogenetic analyses used SSU, LSU and <em>TEF1</em> data in order to confirm the distinctiveness of the new species. The new species presents particular morphological characteristics, such as lacrimoid conidia and white synnemata. Comparison with other species in the genus revealed significant differences both in conidial and synnematal morphology. Based on Maximum Likelihood and Bayesian Inference phylogenies, <em>G. agroflorestalis</em> is a sister species to <em>G. pulchra</em>. The variations observed in the samples may be attributed to the microenvironmental conditions of AFS, which can influence phenotypic expression. This research emphasizes the importance of AFS in biodiversity conservation and in the discovery of new species.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108278"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143229486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanopore reads spanning the whole genome of arthropod-infecting large dsDNA viruses of the class Naldaviricetes enable assembly-free sequence analysis","authors":"Jörg T. Wennmann","doi":"10.1016/j.jip.2025.108277","DOIUrl":"10.1016/j.jip.2025.108277","url":null,"abstract":"<div><div>The nuclear arthropod-specific large DNA viruses comprise different phylogenetically related virus families (<em>Baculoviridae</em>, <em>Nudiviridae</em>, <em>Hytrosaviridae</em> and <em>Nimaviridae</em>) forming the class <em>Naldaviricetes</em>. One common characteristic of their large double-stranded circularly and covalently closed DNA genomes is the size of up to hundreds of kilobase pairs, encoding up to hundreds of open reading frames. Until recently, analyzing such large viral dsDNA genomes was hampered by short read sequencing techniques, requiring bioinformatic assembly strategies to construct a consensus sequence as a descriptor of a population of haplotype sequences. The ideal aim for decoding large dsDNA genomes is an assembly-free method that allows the individual gnomes of a virus population to be sequenced in individual reads without fragmentation. Such full-length genome sequencing of single genome molecules is made possible by Nanopore sequencing, which is increasingly used to decode representatives of the <em>Naldaviricetes</em>. The present study discusses the impact of Nanopore sequencing on the characterization of viral NALDV populations. Full-length genome reads can be found in published sequence data from Bombyx mori nucleopolyhedrovirus (BmNPV; family <em>Baculoviridae</em>) and Oryctes rhinoceros nudivirus (OrNV; family <em>Nudiviridae</em>) isolates, providing insight into the future of deciphering populations of members of the order <em>Lefavirales</em> and class <em>Naldaviricetes</em>. Thirty years after the first sequencing of a baculovirus in 1994, a new era of sequencing of arthropod-infecting large dsDNA viruses has begun, which will allow assembly-free analysis of entire populations of large dsDNA viruses.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108277"},"PeriodicalIF":3.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidate DNA and RNA viruses of Drosophila suzukii from Canada and Germany, and their interactions with Wolbachia","authors":"Jan P. Dudzic ,&nbsp;Audrey E. McPherson ,&nbsp;Keelie E. Taylor ,&nbsp;Astrid Eben ,&nbsp;Paul K. Abram ,&nbsp;Steve J. Perlman","doi":"10.1016/j.jip.2025.108274","DOIUrl":"10.1016/j.jip.2025.108274","url":null,"abstract":"<div><div>Some species of insects harbour strains of the endosymbiotic bacteria <em>Wolbachia</em> that do not cause obvious reproductive manipulations, and so it is unclear why they persist in host populations. There is some evidence that some of these endosymbionts may provide their hosts with protection against viruses, which would help to explain their persistence, but few studies have explored associations between <em>Wolbachia</em> and naturally occurring, common viruses in natural populations. Here, we asked whether individuals of the invasive vinegar fly <em>Drosophila suzukii</em> infected with the <em>wSuz</em> strain of <em>Wolbachia</em> were less likely to be infected by naturally occurring viruses in its invaded range, in western North America and in Europe. First, using next-generation sequencing, we conducted a virome survey of adult and larval <em>D. suzukii</em> in British Columbia, Canada, finding eight candidate RNA viruses and two candidate DNA viruses; all but one have not been reported previously. Only the previously described Teise virus, an RNA virus, was abundant in our virome survey. We then screened individual flies from British Columbia and Germany for Teise virus and <em>Wolbachia</em>. <em>Wolbachia</em>-infected <em>D. suzukii</em> from the field were not less likely to be infected by Teise virus. Overall, our results do not provide conclusive evidence that <em>wSuz</em> provides strong protection for <em>D. suzukii</em> against viruses that are common in natural populations. However, the other viruses that we discovered in this study deserve further characterization in terms of their pathogenicity to <em>D. suzukii</em> and the frequency and dynamics of infection in wild populations.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108274"},"PeriodicalIF":3.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the bacterial pathogen Pseudomonas protegens Pf-5 on the immune response of larvae of the black soldier fly, Hermetia illucens L.","authors":"Parth N. Shah ,&nbsp;Sevasti Maistrou ,&nbsp;Joop J.A. van Loon,&nbsp;Marcel Dicke","doi":"10.1016/j.jip.2025.108272","DOIUrl":"10.1016/j.jip.2025.108272","url":null,"abstract":"<div><div>The larvae of the black soldier fly (BSFL), <em>Hermetia illucens</em> L. (Diptera: Stratiomyidae), are exposed to a diverse range of microorganisms within their feeding substrate that is mainly composed of decaying organic matter. In the current study, we evaluated the effect of an interaction with a Gram-negative bacterium, <em>Pseudomonas protegens</em> Pf-5, on the immune responses of the larvae of <em>H. illucens.</em> Five-day-old BSF larvae were injected with one of five doses of bacterial inoculum to assess survival. We observed dose-dependent mortality in BSF larvae to <em>P. protegens</em> infection, with mortality increasing with an increasing pathogen dose. Injection of more than 50 bacterial cells per larva resulted in 100 % larval mortality, while injection of one bacterial cell per larva caused only 20 % mortality. Phenoloxidase activity, an element of the immune response, correlated with the pathogen dose, increasing early for larvae injected with a high pathogen dose (i.e., 5000 bacterial cells per larva) and later for larvae injected with a low bacterial dose (i.e., one cell per larva). The expression of four genes encoding for antimicrobial peptides (AMPs), namely <em>cecropin, defensin-A, defensin-like peptide 4</em>, and <em>attacin-A</em>, displayed a treatment- and dose-specific expression pattern. Injection with either PBS (control) or different bacterial doses initially induced the upregulation of AMP genes; however, expression reduced over time in the control larvae. At high pathogen dose, all tested genes except <em>hsp70</em> were consistently induced. The expression of all genes, except <em>hsp70</em>, was induced by low pathogen dose at 2 h, then reduced gradually and increased significantly at 15 h. These results collectively indicate that BSF larvae temporally modulate their immune responses, such as phenoloxidase activation and AMP gene expression, to combat a pathogen within their hemolymph.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108272"},"PeriodicalIF":3.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and allele frequencies of genetic variants associated with Varroa drone brood resistance (DBR) in African Apis mellifera subspecies","authors":"Haftom Gebremedhn ,&nbsp;Regis Lefebre ,&nbsp;Moses Chemurot ,&nbsp;Felicien Amakpe ,&nbsp;Bezabeh Amssalu ,&nbsp;Lina De Smet ,&nbsp;Dirk C. de Graaf","doi":"10.1016/j.jip.2025.108276","DOIUrl":"10.1016/j.jip.2025.108276","url":null,"abstract":"<div><div>The ectoparasite <em>Varroa destructor</em> is a major contributor to the global decline of honeybee colonies (<em>Apis mellifera</em>), especially in the Northern Hemisphere. However, <em>Varroa</em>-resistant honeybee populations have been reported in various regions around the globe, including Europe and Africa. This resistance is primarily attributed to the trait known as Suppressed Mite Reproduction (SMR), which significantly reduces the reproductive success of <em>Varroa</em> mites within these colonies. Although this trait is still poorly understood, several efforts have been made to unravel the genetic basis of SMR. For example, a study in Belgium determined eight genetic variants in the honeybee genome that are associated with the infertility of mites in drone brood (Drone Brood Resistance or DBR). As these eight variants were found and validated in subpopulations of European subspecies only, there is limited knowledge about the occurrence of these markers in African honeybees. Hence, this study was designed to determine the allele frequencies of these eight genetic variants in African honeybee populations. More specifically, we used qPCR assays with dual-labeled probes to analyze bee samples collected from Benin, Ethiopia, and Uganda. Our results showed the presence of seven of the eight variants in African <em>Apis mellifera</em> subspecies, which may contribute to their innate resistance against the <em>Varroa</em> mite. Moreover, we found significant differences in allele frequencies among the three sampled African bee populations, suggesting the presence of genetic diversity within these populations, potentially altering their resistance to <em>Varroa</em>. This study revealed similar allele frequencies between African honeybees and bee samples from the European <em>iberiensis</em>-subspecies (A lineage), while Ethiopian bees showed distinct distributions, indicative of a unique lineage. Overall, the occurrence of most DBR-associated genetic variants in African honeybees opens research opportunities to elucidate the predictive properties and potential of these genetic variants in the African continent by examining genotype-phenotype associations.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108276"},"PeriodicalIF":3.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the pathogenicity of Metarhizium to Euschistus heros (Hemiptera: Pentatomidae) eggs","authors":"Aline Nunes da Silva ,&nbsp;Janaína Brandão Seibert ,&nbsp;Jhonattan Rodríguez Guerrero ,&nbsp;Camila Costa Moreira ,&nbsp;Italo Delalibera Júnior","doi":"10.1016/j.jip.2025.108275","DOIUrl":"10.1016/j.jip.2025.108275","url":null,"abstract":"<div><div>The ovicidal effect of entomopathogenic fungi and the mechanisms involved are still debated. The hypothesis that the metabolic activity of germinating conidia can cause insect embryos to become unviable without physical penetration has been proposed. Here, we demonstrated that <em>Metarhizium anisopliae</em> and <em>Metarhizium pingshaense</em> (in a different manner from <em>Beauveria bassiana),</em> reduced the percentage of nymphs hatching to less than 3%. They caused degradation of the egg wall due to the growth and penetration. The visualization of lipids being directed from the eggs towards the interior of the hyphae suggests that the lipid reserve was used as a source of nutrients for the development of these fungi.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108275"},"PeriodicalIF":3.6,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological insight into disseminated neoplasia in Mytilus chilensis from southern Chile","authors":"Karin B. Lohrmann ,&nbsp;Eduardo Bustos ,&nbsp;Rosanna Rojas ,&nbsp;Hugo Robotham ,&nbsp;Ana L. Valdivia ,&nbsp;Florencia Navarrete ,&nbsp;Juan F. Quiroga ,&nbsp;Karin Veloso-Mardones ,&nbsp;Ivonne Lee-Montero","doi":"10.1016/j.jip.2025.108273","DOIUrl":"10.1016/j.jip.2025.108273","url":null,"abstract":"<div><div>This study investigates the prevalence and characteristics of disseminated neoplasia (DN) in <em>Mytilus chilensis</em> in the Los Lagos Region of southern Chile. DN is a malignant proliferative disease that has been described in several species of the genus <em>Mytilus.</em> More than 4.000 adult mussels, both cultured and natural, collected between 2016 and 2022 in different geographical areas (Calbuco-Hueihue, Castro, Quellón) and seasons (winter-spring, summer-autumn) were histologically analysed. The prevalence was assessed and three levels of severity of DN were defined, based on number of neoplastic cells and organs invaded. Morphology and morphometry of neoplastic cells and hemocytes were evaluated. Cell and nucleus diameters and the nucleus/cytoplasm ratio of granulocytes and hyalinocytes were analysed in healthy and neoplastic individuals. The size of neoplastic cells was significantly larger in stage 3 of neoplasia than in stage 1. The overall mean prevalence of DN in <em>M. chilensis</em> was 3.4%, significant differences were found between sites, being higher in Calbuco-Hueihue (5.59%) than in Castro (3.78%) and Quellón (1.40%). The prevalence was significantly higher in the winter-spring season (7.79%) than in the summer-autumn season (3.95%) in Calbuco-Hueihue. The sex, gametogenic stage, and Adipogranular (ADG) cells status were recorded for each mussel, and related to the severity stages of DN. For stages 1 and 2 of DN, females and males could be recognized, but in stage 3 almost no evidence of sex remained. The same trend was identified for the gonadal status, the advanced stages of DN were associated with castration of the mussels. The ADG cells status was highest at stage 1 of DN, declining in number and staining till stage 3. The sex ratio of healthy mussels was 53.5% female, 43.7% male, 1.6% hermaphrodite, and 1.3% indeterminate, for mussels with DN it was 34.1% female, 16% male, 2.2% hermaphrodite and 47.8% indeterminate. Occurrence of DN was higher in females, but the severity stages affected equally female and male mussels. Although the overall prevalence is low, there are some potential risks that warrant continued monitoring and further study of disseminated neoplasia in <em>M. chilensis</em>. This study provides important data on the epidemiology of DN in <em>M. chilensis</em>, which will inform future research and management strategies in aquaculture.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108273"},"PeriodicalIF":3.6,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intramolluscan stages of digeneans parasitizing the pest apple snail Pomacea canaliculata from Argentina: Molecular identification and histopathology","authors":"Lorena E. Martinez ,&nbsp;Carmen M.A. Gilardoni ,&nbsp;Cintia D. Medina ,&nbsp;Silvina Quintana ,&nbsp;Pablo R. Martín ,&nbsp;Florencia Cremonte ,&nbsp;Jorge A. Etchegoin","doi":"10.1016/j.jip.2025.108271","DOIUrl":"10.1016/j.jip.2025.108271","url":null,"abstract":"<div><div><em>Pomacea canaliculata</em> is a highly successful invasive snail that shapes freshwater communities in both native and invaded habitats. We studied its digenean parasites from three freshwater bodies in its native distribution area in Buenos Aires Province, Argentina. An integrated approach was used to determine and describe the larval stages of digenean, including morphological, molecular, and histopathology analyses. We provide the first record of <em>P. canaliculata</em> as the first intermediate host of <em>Stomylotrema vicarium</em> (Stomylotrematidae), two species of the family Phaneropsolidae, and one species of the family Cyclocoelidae. This is also the first record of a species of the family Cyclocoelidae parasitizing snails of the genus <em>Pomacea</em>, with the apple snail acting as both the first and second intermediate host. The digestive gland was identified as the target organ of infection for all species. <em>Stomylotrema vicarium</em> and Phaneropsolidae gen. et sp. 2 alter the structure of the gonads, causing indirect parasitic castration, and, through mechanical compression, destroying also the digestive gland. Adequate knowledge of the identity and dynamics of the parasites affecting <em>P. canaliculata</em> in its native range and the damage they cause is key to explaining the success of this invasive species. The lack of parasite records in invaded areas supports the “enemy release” hypothesis, which could explain the apple snail’s success in these environments. Parasitic castration reduces the reproductive potential of hosts, limiting the expansion and competition of invasive species, making it crucial to understand these impacts for their conservation and control.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108271"},"PeriodicalIF":3.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic entomopathogenic fungi enhance plant immune responses against tomato leafminer","authors":"Sepideh Ghaffari ,&nbsp;Javad Karimi ,&nbsp;Monireh Cheniany ,&nbsp;Alireza Seifi ,&nbsp;Joel Loverodge ,&nbsp;Tariq M Butt","doi":"10.1016/j.jip.2025.108270","DOIUrl":"10.1016/j.jip.2025.108270","url":null,"abstract":"<div><div>Plants employ various defense mechanisms to protect themselves from invaders such as microorganisms and herbivores. By recognizing these threats, plants can trigger a cascade of responses throughout their tissues, effectively priming their defenses and enhancing their resistance to future attacks. In this study, we examined the indirect effects of the entomopathogenic fungi <em>Beauveria bassiana</em> strain GHA and <em>Metarhizium anisopliae</em> strain F01 on tomato growth, expression of selected plant genes, production of secondary metabolites, and preference and performance of the tomato leafminer (<em>Tuta absoluta</em>). Both <em>B. bassiana</em> and <em>M. anisopliae</em> colonized tomato endophytically. Plants treated with <em>B. bassiana</em> had greater biomass than the untreated control and <em>M. anisopliae</em> treated plants. Oviposition was lower on plants treated with <em>B. bassiana</em> and <em>M. anisopliae</em> than on untreated controls in both choice and no-choice studies, and both endophytic EPF also affected the development of leafminer larvae. Gene expression analysis of tomato leaves inoculated with endophytic EPF provided evidence of triggering plant immune response genes, and of priming genes for herbivore attack, making plants more resistant to herbivory. These findings provide important insights into the mechanisms by which <em>B. bassiana</em> and <em>M. anisopliae</em> promote tomato plant growth and rapidly respond to <em>T. absoluta</em> infestation by priming the immune system. This knowledge could improve the development of entomopathogenic fungi for use in plant-protection strategies.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108270"},"PeriodicalIF":3.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}