Journal of invertebrate pathology最新文献

{"title":"Sequence independent immune effects of white spot syndrome virus (WSSV) dsRNA complexed with phytoglycogen nanoparticles in freshwater crayfish","authors":"Kayla A. Samms ,&nbsp;Emma C. Monod ,&nbsp;Aizah Ijaz ,&nbsp;Sarah Au ,&nbsp;Kristof Jenik ,&nbsp;Tania Rodríguez-Ramos ,&nbsp;Brian Dixon ,&nbsp;Stephanie J. DeWitte-Orr","doi":"10.1016/j.jip.2024.108239","DOIUrl":"10.1016/j.jip.2024.108239","url":null,"abstract":"<div><div>White spot syndrome virus (WSSV), a double stranded (ds)DNA virus, is a pathogen that causes massive mortalities in crustaceans worldwide. The present study focuses on using dsRNA to induce sequence-independent immune responses to control virus replication. DsRNA is a well characterized innate immune stimulant in vertebrates and effectively induces an antiviral state. In crustaceans, it has been shown that dsRNA containing WSSV sequences (WSSV-dsRNA) can trigger an immune response independent of RNA interference (RNAi) to mitigate disease. We hypothesized that the potency and efficacy of dsRNA-induced immunity would be enhanced using a biodegradable, cationic phytoglycogen nanoparticle, Nanodendrix (nanoparticle; NP), to deliver the dsRNA. Two <em>in vivo</em> studies were conducted to test the efficacy of long dsRNA as an innate immune stimulant with or without the NP in crayfish. Long dsRNA, 360–500 bp in length, was synthesized based on two WSSV sequences, viral particle 28 (VP28) and viral particle 19 (VP19) respectively. Crayfish were injected in the ventral sinus with WSSV-dsRNA (VP28 or VP19 sequence) either in complex with the NP or alone. High molecular weight (HMW) poly inosinic: polycytidylic acid (poly IC), a synthetic viral dsRNA mimic, was used as a positive control. In the negative control groups, crayfish were injected with either phosphate buffered saline or NP alone. These studies found WSSV-dsRNA could enhance hemocyte numbers, nitric oxide levels and phenoloxidase activity. This enhancement was more effective than when using HMW poly IC. Finally, the nanoparticle did not increase dsRNA’s immune activation capability, but it did reduce dsRNA’s toxicity. Further studies may help determine the efficacy of these treatments as immune stimulants for preventing pathogenic outbreaks in the invertebrate aquaculture industry.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108239"},"PeriodicalIF":3.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-specific patterns of virulence and gene expression profiles of the broad-host-range entomopathogenic fungus Metarhizium anisopliae","authors":"Natasha Sant Anna Iwanicki ,&nbsp;Isabella Alice Gotti ,&nbsp;Italo Delalibera Jr. ,&nbsp;Henrik H. De Fine Licht","doi":"10.1016/j.jip.2024.108242","DOIUrl":"10.1016/j.jip.2024.108242","url":null,"abstract":"<div><div>Generalist pathogens with a broad host range encounter many different host environments. Such generalist pathogens are often highly versatile and adjust their expressed phenotype to the host being infected. Species in the fungal genus <em>Metarhizium</em> (Hypocreales: Clavicipitaceae) occupy various ecological niches, including plant rhizosphere symbionts, soil saprophytes, and insect pathogens with applications in biological control of pests. The species <em>M. anisopliae</em> is highly diverse combining the capability of association with plant roots and infection of a broad range of arachnid and insect hosts, from agricultural pests to vectors of human disease. It is among the most studied and applied biological control agents worldwide. Here, we investigate the phenotypic plasticity and differential gene expression of <em>M. anisopliae</em> blastospores during infection of different insect hosts. First, the virulence of <em>M. anisopliae</em> blastospores was evaluated against <em>Tenebrio molitor</em> (Coleoptera: Tenebrionidae)<em>, Spodoptera frugiperda</em> (Lepidoptera: Noctuidae), <em>Gryllus assimilis</em> (Orthoptera: Gryllidae), and <em>Apis mellifera</em> (Hymenoptera: Apidae)<em>.</em> Second, the percentage of appressorium formation on the membranous wings of the four hosts was determined, and third, the fungal transcriptome profile during penetration on the hosts was analyzed. Our findings reveal that <em>M. anisopliae</em> blastospores exhibit high virulence against <em>Tenebrio molitor</em>, with significantly higher appressorium formation on beetle wings compared to the other three tested insects. We also document distinct gene expression patterns in <em>M. anisopliae</em> blastospores during insect infection of <em>T. molitor, S. frugiperda,</em> and <em>A. mellifera</em>, with notable variations observed in <em>G. assimilis</em>. These differences are associated with the expression of enzymes involved in the degradation of specific compounds present in each insect wing, as well as hydrophobins, destruxins, and specialized metabolites related to virulence. The study emphasizes the differences in fungal gene expression during infection of the four insect orders and highlights the virulence-related genes specific to each infective process.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"209 ","pages":"Article 108242"},"PeriodicalIF":3.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phagostimulant MTSG supplementation benefits growth-promoting and defensive effects of Rhodobacter azotoformans on Chinese mitten crab Eriocheir sinensis","authors":"Xurui Zheng ,&nbsp;Li He ,&nbsp;Lefu Wen ,&nbsp;La Xu ,&nbsp;Youhong Wang ,&nbsp;Haipeng Cao ,&nbsp;Chunlei Gai ,&nbsp;Jian An","doi":"10.1016/j.jip.2024.108240","DOIUrl":"10.1016/j.jip.2024.108240","url":null,"abstract":"<div><div>The use of phagostimulant and <em>Rhodobacter</em> has been well proposed in Chinese mitten crab <em>Eriocheir sinensis</em> culture. Yet the influence of phagostimulant supplementation on the growth-promoting and defensive effects of <em>Rhodobacter</em> on Chinese mitten crabs is still unknown. In this study, a 40-day feeding trial was carried out to examine the contribution of dietary phagostimulant MTSG to the effects of <em>R. azotoformans</em> on the growth performance, immunity, antioxidant capability, intestinal flora diversity, and resistance of <em>E. sinensis</em> to bacterial challenge. The results revealed higher weight gain, specific growth rate, as well as serum and hepatopancreatic acid phosphatase, alkaline phosphatase, superoxide dismutase, and catalase activities in the 3.0 to 9.0 g/kg diet MTSG + <em>R. azotoformans</em>-fed crabs than <em>R. azotoformans</em>-fed crabs, indicating that the supplementation of MTSG at 3.0 to 9.0 g/kg diet could improve the effect of <em>R. azotoformans</em> on growth performance, immunity and antioxidant capability of Chinese mitten crabs. Furthermore, the supplementation of MTSG at 3.0 to 9.0 g/kg diet could strengthen the effect of <em>R. azotoformans</em> on intestinal flora diversity of Chinese mitten crabs, and enhance the effect of <em>R. azotoformans</em> on the disease resistance against <em>S. putrefaciens</em> infection. To the best of our knowledge, this is the first study to reveal the contribution of a phagostimulant (MTSG) to the growth-promoting and defensive effects of <em>R. azotoformans</em> on Chinese mitten crabs.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"208 ","pages":"Article 108240"},"PeriodicalIF":3.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bombyx mori PAT4 gene inhibits BmNPV infection and replication through autophagy","authors":"Kaifang Jia ,&nbsp;Jinyang Wang ,&nbsp;Dan Jiang ,&nbsp;Xiangrui Ding ,&nbsp;Qiaoling Zhao ,&nbsp;Dongxu Shen ,&nbsp;Zhiyong Qiu ,&nbsp;Xuelian Zhang ,&nbsp;Cheng Lu ,&nbsp;Heying Qian ,&nbsp;Dingguo Xia","doi":"10.1016/j.jip.2024.108235","DOIUrl":"10.1016/j.jip.2024.108235","url":null,"abstract":"<div><div>Proton-assisted amino acid transporter 4 (PAT4) is a member of the solute carrier (SLC) 36 family, which mediates the transmembrane transport of amino acids and their derivatives. However, the function of <em>PAT4</em> in <em>Bombyx mori</em> is not clear. In this study, <em>BmPAT4</em> was cloned and identified using PCR technology. Its open reading frame (ORF) includes 1,395 bp, encoding 464 amino acid (Aa). Moreover, the sequence of BmPAT4 has the highest similarity with wild <em>Bombyx.mandarina</em>, <em>Spodoptera frugiperda</em> and <em>Spodoptera litura</em>, and it has ten transmembrane domains. BmPAT4 was localized in the cell membrane and expressed in all tissues of the silkworm. After <em>Bombyx mori</em> nuclear polyhedrosis virus (BmNPV) infection, the expression of <em>BmPAT4</em> in midgut, hemolymph and fat body was significantly up-regulated. In addition, overexpression of <em>BmPAT4</em> in BmN cells could significantly inhibit the proliferation of BmNPV, and the expression of several genes in autophagy pathway decreased significantly. On the contrary, down-regulation of <em>BmPAT4</em> expression by RNA interference can promote BmNPV replication and proliferation, and the expression of key genes in autophagy pathway is significantly increased. This is the first time to report that <em>BmPAT4</em> has an antiviral effect in silkworm. Moreover, the silkworm activates <em>BmTORC1</em> via <em>BmPAT4</em>, which inhibits autophagy in silkworm cells, resulting in a lack of energy and raw materials for BmNPV infection and replication in cells.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"208 ","pages":"Article 108235"},"PeriodicalIF":3.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inheritance of Cry2Ab2 resistance in two Helicoverpa zea (Lepidoptera: Noctuidae) populations resistant to single- and dual-Bacillus thuringiensis proteins","authors":"Fangneng Huang ,&nbsp;Wenbo Yu ,&nbsp;Graham P. Head ,&nbsp;Ying Niu ,&nbsp;Caroline Sakuno ,&nbsp;Shucong Lin ,&nbsp;Tiago Silva ,&nbsp;Bhavana Patla","doi":"10.1016/j.jip.2024.108237","DOIUrl":"10.1016/j.jip.2024.108237","url":null,"abstract":"<div><div>Cry2Ab2 is a <em>Bacillus thuringiensis</em> (Bt) protein that has been pyramided with Cry1A.105 in transgenic maize and Cry1Ac in cotton to control some major lepidopteran pests including the corn earworm/bollworm, <em>Helicoverpa zea</em> (Boddie). However, the widespread occurrence of resistance of this pest to the pyramided Cry1A/Cry2A crops in the southern region of the United State has become a threat to the sustainability of the technology. In this study, multiple genetic crosses and backcrosses were developed to characterize the inheritance of Cry2Ab2 resistance in two <em>H. zea</em> populations resistant to the single protein, Cry2Ab2 (RR_2Ab) and the dual proteins in Bt maize, Cry1A.105/Cry2Ab2 (RR_VT2P). Diet-overlay bioassays with F1 hybrids from reciprocal crosses between a susceptible and the resistant populations showed that the Cry2Ab2 resistance in both RR_2Ab and RR_VT2P was inherited autosomally and non-recessively. Segregation tests in F2 and backcrossed generations indicated that the resistance was likely controlled by more than one locus. The effective dominance levels of the resistance estimated at each of three discriminating concentrations were similar between RR_2Ab and RR_VT2P, ranging from incompletely dominant to incompletely recessive. The similar inheritance observed in RR_2Ab and RR_VT2P suggests that the Cry2Ab2 resistance is independent from the Cry1A.105 resistance. The non-recessive inheritance of the resistance could be an important factor causing the widespread resistance of this insect to the Cry1A/Cry2A crops in the southern region. Implications of the observed inheritance properties in Bt crop resistance management are discussed.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"208 ","pages":"Article 108237"},"PeriodicalIF":3.6,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A histopathological guide for the social spider Stegodyphus dumicola","authors":"Georgina Clark ,&nbsp;Carl N. Keiser ,&nbsp;Steven T. Cassidy ,&nbsp;Desiré Dalton ,&nbsp;Jamie Bojko","doi":"10.1016/j.jip.2024.108236","DOIUrl":"10.1016/j.jip.2024.108236","url":null,"abstract":"<div><div>The study of invertebrate pathology relies on histopathological tools to define visible internal structures and processes in understudied taxa like spiders. Histopathology involves the infiltration of tissues and organ structures with wax or resin, allowing for the visualisation of cellular anatomy and morphological structure, which can lead to the identification of abnormalities (e.g., pathology) and symbioses (e.g., parasites). In this study, southern African social spiders − <em>Stegodyphus dumicola</em> (Araneae: Eresidae) − were histologically prepared whole and their tissue appearances described (eyes, stomach, heart, ovaries, cuticle, stercoral pocket, chelicerae, book lungs, and silk gland). In addition to healthy tissues, an intranuclear, baculovirus-like pathology was identified. The availability of this material and whole-preparation method makes for a valuable histological resource, where few such resources currently exist for spiders.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"208 ","pages":"Article 108236"},"PeriodicalIF":3.6,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Albopleistophora grylli n. gen. n. sp. (Microsporidia) and its impact on crickets (Gryllus spp.) in food-and-feed culture systems","authors":"Edouard Bessette ,&nbsp;Jamie Bojko ,&nbsp;Kelly S. Bateman ,&nbsp;Stuart Ross ,&nbsp;Nicolai V. Meyling ,&nbsp;Bryony A.P. Williams","doi":"10.1016/j.jip.2024.108229","DOIUrl":"10.1016/j.jip.2024.108229","url":null,"abstract":"<div><div>This study provides a comprehensive taxonomic description of a microsporidian parasite infecting crickets, <em>Gryllus bimaculatus</em> and <em>G. assimilis</em>. Our analysis includes gross pathology, histopathology, spore ultrastructure, parasite development cycle, single gene phylogenies, and phylogenomic comparisons. We introduce a new taxon, <em>Albopleistophora grylli</em> n. gen. n. sp., characterised by its unique developmental stages within a sporophorous vesicle, leading to the formation of mature spores measuring 5.7 × 2.8 µm. Although prevalent in commercial cricket cultures, this parasite seemed to have limited effects on cricket survival. Indeed, microsporidia exposure and density assays with the host <em>G. bimaculatus</em>, only revealed density as a significant factor affecting the crickets’ survival. Nevertheless, exposure showed significant effect on the crickets’ emergence time, where exposed crickets emerged as adults earlier than unexposed individuals. Moreover, exposure to the parasite increased the faeces production and weight gain in cricket males. However, neither exposure nor density significantly impacted the females’ fecundity. The absence of spores in non-exposed cricket groups suggested a horizontal transmission, highlighting the importance of controlled rearing practices to eliminate this microsporidium in control groups. The well-known anti-microsporidian drug ‘fumagillin’ was studied with a higher microsporidia dose of exposure to evaluate any improvement in cricket survival, without showing any significant differences between exposed and unexposed groups. Our findings underscore the nuanced dynamics of host-microsporidia interactions and emphasise the need for ecological context in understanding microsporidian impacts. Even if non-dangerous for its host, monitoring of this parasite seems crucial due to its potential zoonotic transmission by its close phylogenomic relation to human-infecting microsporidia species.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"208 ","pages":"Article 108229"},"PeriodicalIF":3.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and genetic diversity of Bombyx mori nucleopolyhedrovirus in mass-reared silkworms in Thailand","authors":"Jörg T. Wennmann ,&nbsp;Sergei Senger ,&nbsp;Birgit Ruoff ,&nbsp;Johannes A. Jehle ,&nbsp;Siripuk Suraporn","doi":"10.1016/j.jip.2024.108221","DOIUrl":"10.1016/j.jip.2024.108221","url":null,"abstract":"<div><div>Silk is an economically and culturally important product of Thailand that is threatened by the outbreaks of the Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm rearings. BmNPV infects the larval stages of the silkworm <em>Bombyx mori</em>, causing fatal viral infection and preventing the larvae from reaching the pupal stage. Numerous BmNPV isolates have been described from silk-producing countries from all over the world. In this study, the geographic distribution of BmNPV in Thailand was analyzed by collecting 18 samples from different regions. The BmNPV samples were analyzed by PCR and whole genome sequencing was performed for eight specimens. Their genome size ranged from 125,888 bp to 126,783 bp, comprising 138 open reading frames. Although the sequenced BmNPV genomes could be phylogenetically differentiated, no correlation between geographic and genetic distance was observed, indicating a close relationship between the BmNPV from Thailand.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108221"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of native Chilean entomopathogenic fungi and their potential for the control of Drosophila suzukii","authors":"Cristian Montalva ,&nbsp;Cristian González ,&nbsp;Cecilia Ruiz ,&nbsp;Isabel Vives ,&nbsp;Andrea Díaz ,&nbsp;Luis Devotto ,&nbsp;Juscelino Rodrigues ,&nbsp;Luiz F.N. Rocha ,&nbsp;Javiera Manríquez ,&nbsp;Tania Vásquez ,&nbsp;Richard A. Humber ,&nbsp;Christian Luz","doi":"10.1016/j.jip.2024.108223","DOIUrl":"10.1016/j.jip.2024.108223","url":null,"abstract":"<div><div><em>Drosophila suzukii</em> is an invasive pest of berries and other soft-skinned fruits, was first detected in Chile in 2017, and has since spread over 2,800 km from north to south. Sustainable control of the spotted-wing drosophila (SWD) is essential due the negative attitude of the consumers toward the excessive use of insecticides. During a survey in Chile for biological control agents, thirty-two isolates of entomopathogenic fungi (EPF) were isolated from mycotized insects and soil samples, identified through sequence analysis, and tested against <em>D. suzukii</em> adults under laboratory conditions. The EPF identified are <em>Akanthomyces muscarius</em>, <em>Beauveria bassiana</em>, <em>Beauveria pseudobassiana</em>, <em>Clonostachys rosea</em>, <em>Metarhizium alvesii</em>, <em>Metarhizium brunneum</em> and <em>Metarhizium robertsii</em>. Six isolates caused 100 % of mortality of <em>D. suzukii</em> adults within ten days after the initial exposition to conidia; these included four <em>B</em>. <em>bassiana</em> isolates (LSB 110, LSB 114, LSB 122 and LSB 125), one <em>M</em>. <em>robertsii</em> isolate (LSB 115) and one <em>M</em>. <em>brunneum</em> isolate (LSB 127). LSB 122 and LSB 125 induced the shortest lethal time (LT₅₀ 4 and 4.2 days, respectively), while <em>Akanthomyces</em> and <em>Clonostachys</em> caused ≤ 36 % cumulative mortality of the adults at 10 days of exposure. These results indicate that isolates of <em>B. bassiana</em>, <em>M. robertsii</em> and <em>M. brunneum</em> have significant potential as microbial control agents against <em>D. suzukii</em> adults. This study marks a critical step forward in identifying and validating native entomopathogenic fungi in Chile for sustainable pest management. Future work will focus on further testing these isolates under laboratory, semi-field and field conditions to optimize their application in real-world agricultural settings.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108223"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of transinfection of Wolbachia from the planthopper Laodelphax striatellus on reproductive fitness and transcriptome of the whitefly Bemisia tabaci","authors":"Hongyan Hu ,&nbsp;Zhenhua Lu ,&nbsp;Yajie Ma ,&nbsp;Xianpeng Song ,&nbsp;Dan Wang ,&nbsp;Changcai Wu ,&nbsp;Xiaoyan Ma ,&nbsp;Yongpan Shan ,&nbsp;Xiangliang Ren ,&nbsp;Yan Ma","doi":"10.1016/j.jip.2024.108230","DOIUrl":"10.1016/j.jip.2024.108230","url":null,"abstract":"<div><div>The whitefly <em>Bemisia tabaci</em> is critical global pest threatening crops and leading to agricultural losses. <em>Wolbachia</em> is an intracellular symbiotic bacterium in insects, which can regulate the growth and development of the host through various ways. In a prior study, <em>Wolbachia</em> was found to be transferred to whitefly and induce fitness changes. However, little is known about the underlying mechanisms of host-<em>Wolbachia</em> interactions in <em>B. tabaci</em>. In this study, a <em>Wolbachia</em> strain <em>w</em>Stri was isolated from the small brown planthopper, <em>Laodelphex striatellus</em>, and transferred to <em>B. tabaci</em>. The distribution of <em>Wolbachia</em> in whiteflies was determined using fluorescence in situ hybridization. Reciprocal crossing experiments demonstrated that <em>w</em>Stri did not induce cytoplasmic incompatibility phenotypes in <em>B. tabaci</em>, but prolonged the developmental duration of the offspring. We performed transcriptomic analysis of <em>Wolbachia</em>-infected female and male adults using Illumina-based RNA-Seq. A total of 843 differentially expressed genes (DEGs) were identified in infected females, among them 141 were significantly up-regulated and 702 were down-regulated by <em>Wolbachia</em> infection. In infected males, of 511 gene sets, 279 host genes were significantly up-regulated, and 232 were down-regulated by <em>Wolbachia</em> infection. KEGG analysis of DEGs demonstrated significant differences in gene pathway distribution between up-regulated and down-regulated genes. These genes are involved in various biological processes, including, but not limited to, detoxification, oxidation–reduction, metabolic processes, and immunity. The transcriptomic profiling of this study offers valuable information on the differential expression of genes in whiteflies following <em>Wolbachia</em> infection, and enhances our understanding of this host-symbiotic interaction.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108230"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}