Journal of invertebrate pathology最新文献_第6页

Ultrastructure of the enlarged labial gland in the ant Camponotus japonicus associated with labial gland disease 日本田鼠唇腺肿大与唇腺疾病相关的超微结构

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-10 DOI: 10.1016/j.jip.2025.108332

Liangliang Zhang , Chu Wang , Lv Yang , Jiangbin Fan , Hong He , Johan Billen

{"title":"Ultrastructure of the enlarged labial gland in the ant Camponotus japonicus associated with labial gland disease","authors":"Liangliang Zhang , Chu Wang , Lv Yang , Jiangbin Fan , Hong He , Johan Billen","doi":"10.1016/j.jip.2025.108332","DOIUrl":"10.1016/j.jip.2025.108332","url":null,"abstract":"<div><div>The labial (salivary) glands of insects play a crucial role in the vectoring of pathogens. Electron microscope observations on enlarged labial gland dissected from minor workers of <em>Camponotus japonicus</em> show a concurrent infection of the labial gland with rod-shaped virus particles and bacteria. Virus particles were found within the cytoplasm, nuclei, and lumen, where they are especially numerous. An apparently multilayered epithelium and gland enlargement are prominent features of the observed condition. The cells of the diseased labial gland reservoir exhibit secretory characteristics and undergo age-related changes. The epithelial thickness reaches its peak approximately one week after emergence and declines thereafter. Similarly, the degree of basement membrane invaginations and the abundance of endoplasmic reticulum in glandular cells followed this same pattern. These observations suggested that biological associations between the labial gland and diverse microbes may be more common than previously recognized.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108332"},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Propolis can reduce Nosema ceranae infection and enhance the immune response in honey bees, without disrupting the gut microbiota 蜂胶可以减少蜜蜂微孔虫感染，增强蜜蜂的免疫反应，而不会破坏肠道微生物群

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-10 DOI: 10.1016/j.jip.2025.108333

Daniela Arredondo , Guillermo Añón , Juan Campá , Jorge Harriet , Pablo Zunino , Karina Antúnez

{"title":"Propolis can reduce Nosema ceranae infection and enhance the immune response in honey bees, without disrupting the gut microbiota","authors":"Daniela Arredondo , Guillermo Añón , Juan Campá , Jorge Harriet , Pablo Zunino , Karina Antúnez","doi":"10.1016/j.jip.2025.108333","DOIUrl":"10.1016/j.jip.2025.108333","url":null,"abstract":"<div><div>Honey bees (<em>Apis mellifera</em>) play a crucial role in the environment, being essential pollinators and contributing to agricultural production and natural ecosystem diversity. However, honey bee health is threatened by multiple stressors, including infection by mites, viruses and microsporidia. To defend against invaders, these insects have evolved social and individual mechanisms, including the collection and use of propolis. In this study, we found that under laboratory conditions, the consumption of Ethanolic Extract of Propolis (EEP) reduced the infection level of the pathogen <em>Nosema ceranae</em> and increased the expression levels of immune-related genes (<em>hymenoptaecin</em> or <em>glucose oxidase</em>) in challenged bees, enhancing their immunocompetence. Moreover, it did not affect the diversity and composition of the gut microbiota. Despite these promising laboratory results, the administration of EEP to honey bee colonies under field conditions did not produce a beneficial effect on colony strength or pathogen infection levels. Future studies should be conducted to optimize the method and frequency of applications to maximize potential benefits for honey bee colony health.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108333"},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Application of Beauveria bassiana conidia to spotted lanternfly forewings causes fewer infections than abdominal applications 球孢白僵菌分生孢子应用于斑点灯笼蝇前翅引起的感染比腹部应用少

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-09 DOI: 10.1016/j.jip.2025.108335

Ann E. Hajek , Thomas A. Everest , Stefan T. Jaronski

引用次数: 0

Infection of two cestode larvae, Nybelinia enterika sp. nov. and Phoreiobothrium sp. in oval squid Sepioteuthis lessoniana species complex 卵形鱿鱼两种幼虫enterbelinia sp. 11和Phoreiobothrium sp.的感染

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-07 DOI: 10.1016/j.jip.2025.108329

Mehmet Arif Zoral , Zdenek Lajbner , Lucia Zifcakova , Shinichi Nakamura , Jonathan Miller

{"title":"Infection of two cestode larvae, Nybelinia enterika sp. nov. and Phoreiobothrium sp. in oval squid Sepioteuthis lessoniana species complex","authors":"Mehmet Arif Zoral , Zdenek Lajbner , Lucia Zifcakova , Shinichi Nakamura , Jonathan Miller","doi":"10.1016/j.jip.2025.108329","DOIUrl":"10.1016/j.jip.2025.108329","url":null,"abstract":"<div><div>Recently, oval squid of <em>Sepioteuthis lessoniana</em> species complex has gained importance in aquaculture due to its successful breeding in semi-intensive and intensive aquaculture systems. However, there is a lack of information regarding pathogens and diseases that can affect this species, prompting the need for further research. Two cestode species, <em>Nybelinia enterika</em> sp. nov. and <em>Phoreiobothrium</em> sp., were identified as members of the Trypanorhyncha and Onchoproteocephalidea orders, respectively, using both morphological and molecular diagnostic markers in wild-caught oval squids (<em>Sepioteuthis lessoniana</em> sp.1, <em>S. lessoniana</em> sp.2, <em>S. lessoniana</em> sp.3). These cestode species were found to use the oval squids as intermediate hosts. In histopathology samples, the infected squid with <em>N. enterika</em> was surrounded by numerous hemocytes in the infected area. In the genomes of both cestode species, we identified multiple genes for enzymes involved in cephalopod tissue degradation, such as cysteine proteases including cathepsin-L, cathepsin-D, cathepsin-A, cathepsin-B, cathepsin-K, and cathepsin-S. These enzymes potentially help the parasites manipulate the squid immune system and neutralize squid hemocytes. The detection of these parasites in wild squid populations, with 100% prevalence in Okinawa, is concerning as the effect of these parasites on human consumers’ health remains unknown. However, our research demonstrated that fully cultured squid remain completely cestode parasite-free, indicating they are more likely to meet the highest standards for food safety and quality.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108329"},"PeriodicalIF":3.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Detection and characterization of white spot syndrome virus in imported blue crayfish (Procambarus alleni) from the ornamental trade 观赏贸易进口蓝螯虾（原螯虾）白斑综合征病毒的检测与鉴定

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-06 DOI: 10.1016/j.jip.2025.108330

Naomi Falconnier , Adrien Izquierdo , Shannon Gray , Nanny Wenzlow , Kuttichantran Subramaniam

引用次数: 0

Experimental infection study reveals differential susceptibilities of Penaeus monodon and Penaeus vannamei to Enterocytozoon hepatopenaei 实验感染研究表明，单对虾和凡纳滨对虾对肝芽胞虫的敏感性存在差异

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-06 DOI: 10.1016/j.jip.2025.108331

B.Naveen Rajeshwar , T.Sathish Kumar , K.P. Jithendran , Megha K. Bedekar , H.Sanath Kumar , K. Jeena , Mujahidkhan A. Pathan , K.V. Rajendran

{"title":"Experimental infection study reveals differential susceptibilities of Penaeus monodon and Penaeus vannamei to Enterocytozoon hepatopenaei","authors":"B.Naveen Rajeshwar , T.Sathish Kumar , K.P. Jithendran , Megha K. Bedekar , H.Sanath Kumar , K. Jeena , Mujahidkhan A. Pathan , K.V. Rajendran","doi":"10.1016/j.jip.2025.108331","DOIUrl":"10.1016/j.jip.2025.108331","url":null,"abstract":"<div><div><em>Enterocytozoon hepatopenaei</em> (EHP) is a significant pathogen affecting penaeid shrimp, particularly farmed whiteleg shrimp <em>Penaeus vannamei</em>, causing hepatopancreatic microsporidiosis (HPM). Although initially reported in tiger shrimp <em>P. monodon</em>, limited data exists on its current impact and pathogenic potential of EHP infection in tiger shrimp due to the shift toward farming <em>P. vannamei</em>. This study aimed to compare the susceptibility of <em>P. monodon</em> and <em>P. vannamei</em> via experimental oral EHP challenge. Challenge group shrimps were fed commercial feed mixed with minced EHP-infected hepatopancreatic tissue (1:1) containing 10⁶ copies/ng DNA for four days, while control groups received commercial pellet feed. Over 90 days, hepatopancreas and fecal samples were aseptically sampled and PCR-tested for EHP presence on days 7, 15, 30, 60, and 90 post-challenge (dpc). EHP loads, quantified using qPCR, were higher in <em>P. vannamei</em> (1.5–5.3 × 10<sup>5</sup> copies/μL DNA) compared to <em>P. monodon</em> (5.6–10.3 × 10<sup>3</sup> copies/μL DNA). Challenged <em>P. monodon</em> tested EHP-positive only in nested step SWP-PCR till 90 dpc. Further, infection was confirmed through wet mount, calcofluor white stain, histology, and <em>in situ</em> hybridization. EHP-challenged <em>P. monodon</em> had a higher survival rate (75 %) than <em>P. vannamei</em> (37.5 %). This first experimental report on EHP in <em>P. monodon</em> indicates it is less susceptible than <em>P. vannamei,</em> suggesting that the reintroduction of <em>P. monodon</em> can help deal with the EHP crisis the shrimp industry is currently going through.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108331"},"PeriodicalIF":3.6,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The activation of caspases in immunocompetent cells is an important infection factor of the pathogenic fungus Conidiobolus coronatus (Entomophthorales: Ancylistaceae) 免疫活性细胞中半胱天冬酶的激活是病原菌冠状孢子虫（Conidiobolus coronatus）的重要感染因子。

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-04 DOI: 10.1016/j.jip.2025.108328

Agata Kaczmarek, Mieczysława Irena Boguś

{"title":"The activation of caspases in immunocompetent cells is an important infection factor of the pathogenic fungus Conidiobolus coronatus (Entomophthorales: Ancylistaceae)","authors":"Agata Kaczmarek, Mieczysława Irena Boguś","doi":"10.1016/j.jip.2025.108328","DOIUrl":"10.1016/j.jip.2025.108328","url":null,"abstract":"<div><div>Apoptosis is a mechanism commonly used by pathogenic fungi to inhibit the host’s immune response. One opportunistic pathogen is <em>Conidiobolus coronatus</em>, which causes fungal infection in mammals and insects. In a study, larvae of <em>Galleria mellonella</em> were exposed to the pathogen for 24 h. After this exposure, some larvae were used for hemolymph collection (F24), while others were incubated for an additional 24 h (F48). The activity of caspase-9 and caspase-3-like proteins in hemocytes was measured using a colorimetric method. The changes in caspase concentration were calculated using ELISA tests. Immunocytochemical analyses were employed to show changes in the levels of the examined proteins in both their pro- and active forms. Fluorescence microscopy was used to detect changes in cultured hemocytes and flow cytometry analysis was conducted to detect both forms of caspases in freshly collected hemocytes. To evaluate the effect of fungal infection, caspase inhibitors (Z-DEVD-FMK and Z-LEHD-FMK) were injected into the larvae, and their impact on insect development and resistance to fungal infection was determined. The exposure of larvae to the entomopathogen increased the detection levels, concentrations, and activity of both caspase-like proteins in hemocytes during fungal infection. The research has indicated that inhibition of these proteins disrupts larval development and increases resistance to infection. These results suggest that apoptosis might be an important mechanism for a pathogen to inhibit the insect immune response. Given the similarities between insects’ and mammals’ innate immune responses, the presented results may indicate a potential mechanism of fungal pathogenicity in both groups.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108328"},"PeriodicalIF":3.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Fungal-bacterial interactions leading to increased insect mortality: Trichoderma xixiacum enhances the pathogenicity of Serratia marcescens in Protaetia brevitarsis larvae 真菌与细菌的相互作用导致昆虫死亡率增加：西霞木霉增强了短维弧菌幼虫中粘质沙雷氏菌的致病性

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-04 DOI: 10.1016/j.jip.2025.108327

Abdelaziz Mansour , Taeho Jeong , Duyoung Lee , Jeong-Hun Song , Eunsun Kim , Dae-Weon Lee , Mohamed Mannaa , Young-Su Seo

{"title":"Fungal-bacterial interactions leading to increased insect mortality: Trichoderma xixiacum enhances the pathogenicity of Serratia marcescens in Protaetia brevitarsis larvae","authors":"Abdelaziz Mansour , Taeho Jeong , Duyoung Lee , Jeong-Hun Song , Eunsun Kim , Dae-Weon Lee , Mohamed Mannaa , Young-Su Seo","doi":"10.1016/j.jip.2025.108327","DOIUrl":"10.1016/j.jip.2025.108327","url":null,"abstract":"<div><div>Edible insect farming presents significant potential as a sustainable solution to meet the increasing demand for protein and biowaste recycling. However, microbial contamination in rearing substrates poses a serious threat to insect populations. This study investigates the pathogenicity of <em>Serratia marcescens</em> YSMM-S1, isolated from dead <em>Protaetia brevitarsis</em> larvae, and explores its interaction with <em>Trichoderma xixiacum</em>, a fungus co-isolated from the same rearing environment. The identified <em>S. marcescens</em> YSMM-S1 was confirmed as a highly virulent strain, causing 100 % larval mortality within 24 h. In addition, <em>T. xixiacum</em> identified via extensive molecular and morphological characterization, is reported here for the first time in Korea. Co-cultivation and mycophagy assays revealed a significant increase in <em>S. marcescens</em> proliferation in the presence of <em>T. xixiacum</em>, likely through fungal mycelium consumption. Moreover, preinoculation with <em>T. xixiacum</em> in oak-fermented sawdust significantly enhanced <em>S. marcescens</em> population growth and colonization. <em>In vivo</em> assays further demonstrated that co-inoculation of the substrate with both microorganisms resulted in significantly higher larval mortality compared to single inoculations. This study provides novel insights into the complex microbial dynamics in insect-rearing systems, emphasizing the role of <em>T. xixiacum</em> in facilitating bacterial pathogenicity. The findings highlight the need for careful microbial management to prevent outbreaks in mass-rearing environments. This work also contributes to the understanding of fungal-bacterial interactions in insect farming and offers new perspectives on the ecological roles of <em>Trichoderma</em> species in rearing substrates.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108327"},"PeriodicalIF":3.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Enterocytozoon hepatopenaei infection stages in shrimp using machine learning and gene network analysis 利用机器学习和基因网络分析表征对虾肝芽胞虫感染阶段

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-03 DOI: 10.1016/j.jip.2025.108326

Yi Qiao , Leiting Zhang , Xiaohui Cao , Tingyue Liu , Huiwen Jiang , Jie Cheng , Ge Jiang , Xianping Fan , Hui Shen

{"title":"Characterization of Enterocytozoon hepatopenaei infection stages in shrimp using machine learning and gene network analysis","authors":"Yi Qiao , Leiting Zhang , Xiaohui Cao , Tingyue Liu , Huiwen Jiang , Jie Cheng , Ge Jiang , Xianping Fan , Hui Shen","doi":"10.1016/j.jip.2025.108326","DOIUrl":"10.1016/j.jip.2025.108326","url":null,"abstract":"<div><div><em>Enterocytozoon hepatopenaei</em> (EHP), causing hepatopancreatic microsporidiosis (HPM), significantly impacts <em>Litopenaeus vannamei</em>, leading to economic losses. Using bioinformatics and machine learning, this study characterized EHP infection stages and host-pathogen interactions. Consensus clustering of 2,613 metabolism-related genes from 36 shrimp samples identified four subclasses: healthy (HG), heavily (HEG), moderately (MEG), and lightly infected groups (LEG). Gene Set Variation Analysis (GSVA) revealed subclass-specific metabolic and immune patterns, with HEG showing impaired carbohydrate metabolism and upregulated amino acid degradation, MEG indicating recovery, and LEG demonstrating metabolic normalization. Weighted Gene Co-expression Network Analysis (WGCNA) linked infection subclasses to pathways like Hippo, JAK-STAT, steroid biosynthesis, and calcium signaling. Machine learning identified 52 characteristic genes involved in EHP proliferation (e.g., RAPTOR), host invasion (e.g., cell surface glycoprotein 1), and host defense (e.g., mucin-5AC). A stacked classifier model predicted infection severity with high accuracy. EHP severely impacts immunity, autophagy, and oxidative stress in early infection, with host responses evolving from detoxification to metabolic recovery and adaptation. Key pathways and genes, including polar tube protein (PTP) and mucin-5AC, were identified as critical to host-pathogen interactions, offering insights into EHP infection dynamics and potential intervention strategies.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108326"},"PeriodicalIF":3.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Viral mutations and their implications for genetic diversity of the Infectious Myonecrosis Virus (IMNV) 病毒突变及其对传染性肌坏死病毒（IMNV）遗传多样性的影响

IF 3.6 3区生物学

Journal of invertebrate pathology Pub Date : 2025-04-03 DOI: 10.1016/j.jip.2025.108325

Jhonatas Teixeira Viana , Juliana Oliveira de Freitas , Rafael dos Santos Rocha , Rodrigo Maggioni

引用次数: 0