Journal of invertebrate pathology最新文献

{"title":"Antisense DNA acaricide targeting pre-rRNA of two-spotted spider mite Tetranychus urticae as efficacy-enhancing agent of fungus Metarhizium robertsii","authors":"Daria Gavrilova ,&nbsp;Ekaterina Grizanova ,&nbsp;Ilia Novikov ,&nbsp;Ekaterina Laikova ,&nbsp;Alexandra Zenkova ,&nbsp;Vladimir Oberemok ,&nbsp;Ivan Dubovskiy","doi":"10.1016/j.jip.2025.108297","DOIUrl":"10.1016/j.jip.2025.108297","url":null,"abstract":"<div><div>Two-spotted spider mite <em>Tetranychus urticae</em> Koch (Acari: Tetranychidae) is one of the most dangerous pests in the world and one of the most pesticide-resistant species ever. Complex biological preparations are of great interest for the acaricide market because they do not poison ecosystems and do not bioaccumulate in food products, simultaneously, pests more slowly develop resistance to complex preparations. In this study we applied complex bioformulation composed of 11-mer antisense oligonucleotide (oligonucleotide acaricide or DNA acaricide) Tur-3 and fungus <em>Metarhizium robertsii</em> for <em>T. urticae</em> control<em>.</em> We discovered that joint contact application of DNA acaricide and fungus <em>M. robertsii</em> significantly attenuates reproduction rate of the mite. Our results indicate that DNA acaricide Tur-3 and fungus <em>M. robertsii</em> act synergistically and lead to a significant 7-times elevated mortality rate and a reduction of 80% in the mite fecundity. Oligonucleotide acaricide Tur-3 causes 2.5-fold reduction of expression of the target pre-rRNA of <em>T. urticae</em> and suppresses the activity of key players of detoxifying enzymes of its defense system (phenoloxidase, esterase, glutathione-S-transferase), on average, by 2–3 fold. Oligonucleotide acaricide Tur-3 interferes with protein biosynthesis causing decrease in production of defense system enzymes of the pest. Obviously, attenuation of defense system enhances fungal infection or/and fungus produces a variety of enzymes that degrade the integument structure of the pest, aiding the penetration of oligonucleotide acaricide Tur-3. This research article is the first evidence of successful application of oligonucleotide acaricide together with fungus <em>M. robertsii</em> for efficient <em>T. urticae</em> control. Combined application of oligonucleotide acaricides based on conservative antisense sequences of rDNAs of pests and fungi is a potent and selective approach for eco-friendly plant protection.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108297"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in-depth study of the growth inhibition of Vibrio parahaemolyticus by Surfactin and its effects on cell membranes, ROS levels and gene transcription","authors":"Zijie Zhou ,&nbsp;Luoping Xiang ,&nbsp;Xiaotong Wang ,&nbsp;Ge Jiang ,&nbsp;Jie Cheng ,&nbsp;Xiaohui Cao ,&nbsp;Xianping Fan ,&nbsp;Hui Shen","doi":"10.1016/j.jip.2025.108298","DOIUrl":"10.1016/j.jip.2025.108298","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) caused by <em>Vibrio parahaemolyticus</em> (V<em>p_AHPND</em>) poses a significant challenge to the shrimp farming industry. Although lipopeptides produced by <em>Bacillus subtilis</em> have been shown to exert strong inhibitory effects against <em>Vibrio parahaemolyticus</em>, the underlying mechanisms remain largely unexplored. This study reveals that the lipopeptide surfactin, produced by <em>Bacillus subtilis</em>, significantly inhibits the V<em>p_AHPND</em> strain JSHY-1669 through multiple mechanisms. Using antagonistic assays and transcriptomic analysis, this paper investigates the molecular mechanisms of surfactin’s inhibitory action on V<em>p_AHPND</em> strain JSHY-1669. The minimum inhibitory concentration (MIC) of surfactin against JSHY-1669 was determined to be 0.125 mg/mL, with a cumulative inhibitory effect. Exposure to surfactin caused significant structural damage to the bacterial cells, markedly inhibiting their growth and virulence gene expression. Transcriptomic analysis identified 64 genes with significant differential expression, including upregulation of genes involved in key metabolic pathways such as carbohydrate transport, and downregulation of non-essential pathways like amino acid and sulfur metabolism. Surfactin affects JSHY-1669 by disrupting key physiological processes. Specifically, it increases cell membrane depolarization, reactive oxygen species (ROS) production, and malondialdehyde (MDA) levels. These changes collectively lead to the loss of membrane integrity, which ultimately inhibits bacterial growth. Additionally, the study found downregulation of slyA, a key regulatory factor related to DNA-binding transcription, virulence regulation, and carbohydrate metabolism. Surfactin may expand its inhibitory range by affecting the slyA regulatory network, providing a basis for surfactin’s broader antibacterial targets. These findings elucidate the inhibitory mechanisms of surfactin on V<em>p_AHPND</em> strain JSHY-1669, laying a foundation for its potential applications.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108298"},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"South American invertebrate pathology: An editorial for the special issue","authors":"Florencia Cremonte ,&nbsp;Nuria Vázquez ,&nbsp;Jamie Bojko","doi":"10.1016/j.jip.2025.108300","DOIUrl":"10.1016/j.jip.2025.108300","url":null,"abstract":"<div><div>South America has extraordinary biodiversity, with an immense variety of invertebrate species playing a critical role in ecosystems. Molluscs and crustaceans support fisheries and aquaculture worldwide, but their populations are increasingly threatened by pathogens. Understanding host-parasite interactions is essential for managing disease outbreaks and ensuring the sustainability of both natural ecosystems and aquaculture systems. Bivalves, gastropods and crustaceans are hosts to numerous lineages of parasites, highlighting the interactions that shape ecological dynamics and influence both conservation efforts and disease management strategies.</div><div>This special issue presents a range of studies pertaining to invertebrate pathology from the South American region, including viral, protozoan and metazoan diseases across a wide range of host species. It also explores advances in pathology, immunology, genomics, phylogenetic and ecological associations.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108300"},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin-amended media: Improving efficacy of Cordyceps fumosorosea as a control agent of Cydia pomonella","authors":"Nushrat Harun Antara,&nbsp;Dietrich Stephan","doi":"10.1016/j.jip.2025.108296","DOIUrl":"10.1016/j.jip.2025.108296","url":null,"abstract":"<div><div>The microbial control of insect pests such as the codling moth <em>Cydia pomonella</em> is a major challenge in the apple industry. This study investigates the potential of chitin-amended media to improve the efficacy of the entomopathogenic fungus <em>Cordyceps fumosorosea</em> strain JKI-BI-1496 for control of <em>C. pomonella</em>. Chitin, a key component of insect exoskeletons, colloidal chitin (CC) or chitosan were added in two concentrations to the liquid growth media. Only by the amendment of 0.5 % chitosan significant higher numbers of spores were formed whereas the highest biomass was achieved by amendment of chitin. Spores produced in chitosan had no significant effect on the mortality of <em>C. pomonella</em>. But media containing 1 % colloidal chitin (CC) significantly enhanced the efficacy of <em>C. fumosorosea.</em> To achieve 75 % mortality only 1.6 × 10⁷ submerged spores/mL were needed in comparison of over four times higher concentrations in media without CC or chitin. However, while 1 % CC positively impacted spore viability during the freeze-drying formulation process, it had a detrimental effect on the fungus’s resilience under prolonged sunlight exposure. These findings provide valuable insights into optimizing media and formulations to enhance the effectiveness of <em>C. fumosorosea</em> in biological control, particularly by improving spore production and formulation processes.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108296"},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The bacterial symbionts of Entomopathogenic nematodes and their role in symbiosis and pathogenesis","authors":"S. Patricia Stock ,&nbsp;Selçuk Hazir","doi":"10.1016/j.jip.2025.108295","DOIUrl":"10.1016/j.jip.2025.108295","url":null,"abstract":"<div><div>Entomopathogenic bacteria in the genera <em>Xenorhabdus</em> and <em>Photorhabdus</em> are mutualistically associated with entomopathogenic nematodes (EPN) <em>Steinernema</em> and <em>Heterorhabditis</em>, respectively. Together they form an insecticidal partnership which has been shown to kill a wide range of insect species. The spectrum of dependence in this symbiotic partnership is diverse, ranging from a tight, obligate relationship to a facultative one. A body of evidence suggests that the reproductive fitness of the nematode-bacterium partnership is tightly associated and interdependent. Furthermore, maintenance of their virulence is also critical to the conversion of the insect host as a suitable environment where this partnership can be perpetuated. Disruption of the symbiotic partnership can have detrimental effects on the fitness of both partners. The nematode-bacterial symbiont-insect partnership represents a model system in ecology and evolutionary biology and amenable to investigate beneficial and antagonistic interactions between invertebrates and microbes. Furthermore, the EPN’s bacterial symbionts are also viewed as a model system to study the biosynthesis, structure and function of various natural products. Their ability to produce up to 25 different natural product classes is outstanding among the Morganellaceae. These natural products show biological activity, most likely originating from important functions during the life cycle of both the nematodes and their symbionts. Tools and high throughput technologies have been developed to identify ubiquitous and rare molecules and study their function and assess their potential as novel biological activities. We herein summarize the symbiotic relationship between EPN and their bacterial symbionts, focusing on their fitness and their ability to successfully access and utilize an insect host. We also recapitulate the history of natural products research highlighting recent findings and the synthetic biology approaches that are currently implemented to identify non-natural derivatives from <em>Xenorhabdus</em> and <em>Photorhabdus</em> with improved biological activity.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108295"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification a novel syntaxin-like protein from silkworm Bombyx mori pathogen Nosema bombycis and characteristics its membrane fusion function","authors":"Liuyi Hong ,&nbsp;Qing Lv ,&nbsp;Hongjie Liao ,&nbsp;Zhengkai Xie ,&nbsp;Maoping Shao ,&nbsp;Yuanyu Zhang ,&nbsp;Yuqing Chen ,&nbsp;Guoqing Pan ,&nbsp;Mengxian Long ,&nbsp;Zeyang Zhou","doi":"10.1016/j.jip.2025.108292","DOIUrl":"10.1016/j.jip.2025.108292","url":null,"abstract":"<div><div>Pebrine is a serious disease of the silkworm, <em>Bombyx mori</em>, caused by the first identified microsporidium <em>Nosema bombycis</em>, which is an obligate parasitic single-celled eukaryote. The pathogen can spread both horizontally and vertically, severely affecting sericulture. SNARE proteins mainly mediate the transport of vesicles and membrane fusion, playing a key role in the biological processes. The microsporidium is known to have a well-developed membrane system, especially the polaroplast which occupies most of the volume of mature spores. In order to explore the function of microsporidian SNARE protein, the transcription and subcellular localization characteristics of a novel Syntaxin-like protein (NbSTX-like) from <em>N. bombycis</em> that had a conserved <em>t</em>-SNARE motif were analyzed. In the different development stages of <em>N. bombycis</em>, the NbSTX-like expressed in the nucleus of meronts, then transited to the cytoplasm in the sporonts, gradually gathered at the two ends of the sporoblasts, and finally concentrated at the polaroplast, posterior vacuole and plasma membrane region of mature spores. Interestingly, the rNbSTX-like protein could fuse liposomes to form large vesicular and tubular structures. The formation of sporoplasms was inhibited by the anti-NbSTX-like serum, implying that NbSTX-like protein participated in sporoplasm maturation. These findings laid a foundation for studying the function of SNARE proteins in microsporidia and provided new insights for the prevention and control of sericulture pathogens.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108292"},"PeriodicalIF":3.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of a new microsporidium, Neoflabelliforma leuchtenbergianum n. sp. from the adipose tissue of Diaphanosoma leuchtenbergianum (Crustacea: Sididae) in China","authors":"Chuanzheng Yang ,&nbsp;Mei Cao ,&nbsp;Chang Wang ,&nbsp;Xunbo Miao ,&nbsp;Chenxi Zhang ,&nbsp;Rulin Chen ,&nbsp;Jingrong Zhang ,&nbsp;Jinyong Zhang ,&nbsp;Meiqi Weng ,&nbsp;Xingqiang Wang","doi":"10.1016/j.jip.2025.108294","DOIUrl":"10.1016/j.jip.2025.108294","url":null,"abstract":"<div><div>This study describes a new microsporidian species from the adipose tissue of <em>Diaphanosoma leuchtenbergianum</em> collected from a eutrophic water body of Lianyungang city, Jiangsu province, China. Infected cladocerans exhibited opacity due to the accumulation of numerous spores in the adipose tissue. The earliest stages observed were uninucleate meronts which were in direct contact with the host cell cytoplasm. Multinucleate sporogonial plasmodia underwent plasmotomy to form uninucleate sporoblasts. Mature spores were ovoid, monokaryotic, and measured 4.80 ± 0.37 (4.09–5.23) µm in length and 2.64 ± 0.15 (2.34–3.01) µm in width. The polaroplast was bipartite with the tightly packed anterior lamellae and posterior tubules. The isofilar polar filament coiled in 9–10 turns and arranged in 2–3 rows. The exospore was covered with tubular projections and consisted of four layers. Phylogenetic analysis based on the SSU rDNA sequence indicated that the present species clustered with two <em>Neoflabelliforma</em> species (<em>N. dubium</em> from freshwater oligochaetes and <em>N. aurantiae</em> from freshwater daphnia) and an unidentified microsporidium from soil with high support values to form a solitary branch in microsporidia. Based on the morphological characteristics, ultrastructural features, and SSU rDNA-inferred phylogeny, a new species was named as <em>Neoflabelliforma leuchtenbergianum</em> n. sp.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108294"},"PeriodicalIF":3.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of a microsporidian parasite in different social forms of the red imported fire ant, Solenopsis invicta, in its native range in Argentina","authors":"Marina S. Ascunce ,&nbsp;Luis Calcaterra ,&nbsp;DeWayne Shoemaker","doi":"10.1016/j.jip.2025.108288","DOIUrl":"10.1016/j.jip.2025.108288","url":null,"abstract":"<div><div>We surveyed 559 colonies of the fire ant <em>Solenopsis invicta</em> in 15 sites in its native range in Argentina for the microsporidian parasite <em>Kneallhazia solenopsae</em>, a natural enemy of this ant. The microsporidium was detected in 20 out of 508 monogyne and nine out of 51 polygyne colonies corresponding to mean infection prevalences of 3.31% and 13.68%, respectively. In the USA, previous studies have shown that <em>K. solenopsae</em> prevalence in the polygyne social form is variable, and as high as 100% in certain sites, whereas infections in the monogyne social form in the USA are rare. In this study, we found that the <em>K. solenopsae</em> genetic variant in the USA is genetically different from the variants identified in the native range, supporting the idea that the USA variant may only infect polygyne colonies.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108288"},"PeriodicalIF":3.6,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and functional analysis of the small heat shock protein HSP19.5 in Bombyx mori in response to Nosema bombycis infection","authors":"Zhenghao Han ,&nbsp;Shidong Jiang ,&nbsp;Jingxian Xie ,&nbsp;Ibouanga Sama Lucreche Poupina ,&nbsp;Xiaoli Mo ,&nbsp;Li Sui ,&nbsp;Ping Qian ,&nbsp;Xudong Tang","doi":"10.1016/j.jip.2025.108289","DOIUrl":"10.1016/j.jip.2025.108289","url":null,"abstract":"<div><div>Small heat shock proteins (sHSPs) are molecular chaperones known for their role in maintaining cellular homeostasis and protecting cells from various environmental stresses. This study focuses on the silkworm small heat shock protein HSP19.5 and its potential functions in the context of <em>Nosema bombycis</em> infection, a microsporidian pathogen causing severe disease in the sericulture industry. We cloned and characterized <em>HSP19.5</em> and revealed its expression patterns in different silkworm tissues and developmental stages. Our results indicate that <em>HSP19.5</em> expression is significantly up-regulated in response to <em>N. bombycis</em> infection, suggesting a role in the host stress response. Through a series of experiments, including RNA interference and overexpression analyses, we demonstrated that HSP19.5 promotes <em>N. bombycis</em> proliferation, possibly by inhibiting host cell apoptosis and regulating intracellular ROS levels. The cytoplasmic localization of HSP19.5 in silkworm cells is consistent with its function as a molecular chaperone. The results enhance our understanding of the complex host-pathogen interactions between silkworms and <em>N. bombycis</em>, and provides insights that may inform the development of novel strategies to control the pebrine disease.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108289"},"PeriodicalIF":3.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus thuringiensis-derived pesticidal proteins toxic to the whitefly, Bemisia tabaci","authors":"Clebson S. Tavares ,&nbsp;Xinyue Wang ,&nbsp;Saptarshi Ghosh,&nbsp;Ruchir Mishra,&nbsp;Bryony C. Bonning","doi":"10.1016/j.jip.2025.108291","DOIUrl":"10.1016/j.jip.2025.108291","url":null,"abstract":"<div><div>The whitefly, <em>Bemisia tabaci</em>, is among the most important threats to global agriculture and food security. In addition to losses associated with feeding, <em>B. tabaci</em> vectors hundreds of plant viruses, many of which cause severe disease in staple food crops. The management of <em>B. tabaci</em> is confounded by extensive resistance to chemical insecticides. While pesticidal proteins derived from entomopathogenic bacteria such as <em>Bacillus thuringiensis</em> (Bt) could provide for alternative management approaches, only one pesticidal protein with toxicity to <em>B. tabaci</em> has been identified. Here we screened 11 Bt-derived pesticidal proteins from several different structural classes against the highly invasive, Middle East-Asian Minor 1 (MEAM1) cryptic species of <em>B. tabaci</em>, and assessed the impact of a <em>B. tabaci</em>-active protein on the gut epithelial membrane by transmission electron microscopy. The pesticidal proteins were expressed in Bt or in <em>Escherichia coli</em> and purified for use in bioassays. The toxicity of purified proteins was first assessed by feeding adults on a single dose followed by lethal concentration (LC₅₀) determination for proteins with significant mortality relative to the buffer control. The proteins Tpp78Aa1, Tpp78Ba1, and Cry1Ca were toxic to <em>B. tabaci</em> with LC₅₀ values of 99, 96, and 351 µg/mL, respectively. Disruption of the brush border and severe reduction in microvilli on the gut surface caused by Tpp78Aa1 is consistent with the mode of action of Bt-derived pesticidal proteins. These proteins may provide valuable tools for the integrated management of <em>B. tabaci</em> populations and associated reduced incidence of <em>B. tabaci</em> vectored plant viral diseases.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108291"},"PeriodicalIF":3.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}