{"title":"两种呼肠孤病毒在柑橘地虱连续细胞系中的复制","authors":"Saptarshi Ghosh, Ke Wu, Bryony C. Bonning","doi":"10.1016/j.jip.2025.108421","DOIUrl":null,"url":null,"abstract":"<div><div>The ability of <em>Diaphorina citri</em> cell lines to support the replication of viruses was assayed using virus stocks enriched from Florida, USA and Taiwan psyllid populations that contained six of the seven <em>D. citri</em> viruses described to date. This study provides conclusive evidence for the replication of two reoviruses (D. citri Cimodo-like virus and <em>D. citri reovirus</em>; DcRV) from the Florida virus stock in Dici3 and to a lesser extent in the Dici3F cell line. The other four viruses were either not detected or did not replicate in either cell line. Unexpectedly, none of the viruses from the Taiwan sample, including DcRV replicated in Dici3 cells.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"213 ","pages":"Article 108421"},"PeriodicalIF":2.4000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Replication of two reoviruses in continuous cell lines derived from Diaphorina citri\",\"authors\":\"Saptarshi Ghosh, Ke Wu, Bryony C. Bonning\",\"doi\":\"10.1016/j.jip.2025.108421\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The ability of <em>Diaphorina citri</em> cell lines to support the replication of viruses was assayed using virus stocks enriched from Florida, USA and Taiwan psyllid populations that contained six of the seven <em>D. citri</em> viruses described to date. This study provides conclusive evidence for the replication of two reoviruses (D. citri Cimodo-like virus and <em>D. citri reovirus</em>; DcRV) from the Florida virus stock in Dici3 and to a lesser extent in the Dici3F cell line. The other four viruses were either not detected or did not replicate in either cell line. Unexpectedly, none of the viruses from the Taiwan sample, including DcRV replicated in Dici3 cells.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":\"213 \",\"pages\":\"Article 108421\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201125001557\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201125001557","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Replication of two reoviruses in continuous cell lines derived from Diaphorina citri
The ability of Diaphorina citri cell lines to support the replication of viruses was assayed using virus stocks enriched from Florida, USA and Taiwan psyllid populations that contained six of the seven D. citri viruses described to date. This study provides conclusive evidence for the replication of two reoviruses (D. citri Cimodo-like virus and D. citri reovirus; DcRV) from the Florida virus stock in Dici3 and to a lesser extent in the Dici3F cell line. The other four viruses were either not detected or did not replicate in either cell line. Unexpectedly, none of the viruses from the Taiwan sample, including DcRV replicated in Dici3 cells.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.