Journal of invertebrate pathology最新文献

{"title":"Crayfish pet trade as a pathway for the introduction of known and novel viruses","authors":"Katarina Bačnik ,&nbsp;Luka Kranjc ,&nbsp;Leticia Botella ,&nbsp;Ivana Maguire ,&nbsp;Dora Pavić ,&nbsp;Jiří Patoka ,&nbsp;Paula Dragičević ,&nbsp;Martin Bláha ,&nbsp;Ana Bielen ,&nbsp;Antonín Kouba ,&nbsp;Denis Kutnjak ,&nbsp;Sandra Hudina","doi":"10.1016/j.jip.2025.108345","DOIUrl":"10.1016/j.jip.2025.108345","url":null,"abstract":"<div><div>Expanding international pet trade has emerged as one of the main introduction pathways of aquatic invasive species, with ornamental crayfish species commonly available on the EU and global markets. Besides most frequently studied crayfish pathogens, such as <em>Aphanomyces astaci</em> and white spot syndrome virus (WSSV), ornamental crayfish carry associated microbial communities, which may potentially lead to the emergence of known or even novel diseases following intentional or unintentional release of animals into the wild. This is especially problematic in the case of viruses, which represent an important, yet considerably understudied, group of crayfish pathogens. Here we analyzed viromes of hepatopancreas tissue of four crayfish species acquired in the international pet trade in Europe (<em>Procambarus clarkii, Procambarus alleni</em>, <em>Cherax holthuisi</em>, and <em>Cherax quadricarinatus</em>) using a high throughput sequencing based metagenomic approach. Seven different known viruses were identified, which were previously either directly associated with crayfish (WSSV, Cherax quadricarinatus reovirus, chequa iflavirus, athtab bunya-like virus) or with hosts from subphylum Crustacea or invertebrates associated with freshwater environment (Shahe ispoda virus 5, <em>Dicistroviridae</em> sp.). Additional sequences represented 8 potential novel and divergent RNA viruses, most similar to sequences belonging to members of <em>Picornavirales, Elliovirales, Reovirales, Hepelivirales, Tolivirales</em> and <em>Ghabrivirales</em> orders. We discuss our findings in relation to their phylogenetic relationships, geographical origins, and putative pathogenicity implications. The results highlight the need for further research into the risks related to disease emergence associated with the pet trade.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108345"},"PeriodicalIF":3.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and genome sequence analysis of a novel alphabaculovirus isolated from sweet potato leaf worm, Aedia leucomelas","authors":"Jian Yang,&nbsp;Zhigao Zhan,&nbsp;Wendong Kuang,&nbsp;Jinchang Wang","doi":"10.1016/j.jip.2025.108342","DOIUrl":"10.1016/j.jip.2025.108342","url":null,"abstract":"<div><div>Sweet potato leaf worm (Aedia leucomelas; Lepidoptera: Noctuidae) adversely affects sweet potato quality and yield. We collected infected A. leucomelas larvae with typical symptoms of baculovirus infection in Fuzhou, China in 2023 and then isolated a baculovirus, which we named Aedia leucomelas nucleopolyhedrovirus (isolate Fuzhou;AeleNPV-FZ). <em>Helicoverpa armigera</em> and <em>Spodoptera exigua</em> larvae were also susceptible to AeleNPV-FZ in laboratory assays, suggesting that AeleNPV-FZ may be useful for controlling multiple noctuid pests under field conditions. The AeleNPV-FZ genome was sequenced and de novo assembled. The genome was 135,409 bp in length with 146 open reading frames (ORFs) and 12 homologous regions (hrs) annotated for the sequence. Phylogenetic relationships inferred from concatenated core gene amino acid sequence alignment and pairwise Kimura two-parameter distances indicated that AeleNPV-FZ may be a novel member of genus <em>Alphabaculovirus</em>.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108342"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Tuta absoluta virome reveals higher viral diversity in field populations","authors":"Rosa Esmeralda Becerra-García,&nbsp;Luis Hernández-Pelegrín,&nbsp;Cristina M Crava,&nbsp;Salvador Herrero","doi":"10.1016/j.jip.2025.108340","DOIUrl":"10.1016/j.jip.2025.108340","url":null,"abstract":"<div><div>A significant number of insect-specific viruses (ISVs) have been discovered in agriculturally important insect pests, facilitated by high-throughput sequencing (HTS). Despite its global impact on tomato crops, the RNA virome of the South American tomato pinworm, <em>Tuta absoluta</em>, remains uncharacterized. In this study, we utilized <em>meta</em>-transcriptomics and bioinformatic approaches to discover the RNA virome of <em>T. absoluta</em> across worldwide populations. We identified ten novel ISVs, classified into eight groups: <em>Nidovirales</em>, <em>Bunyavirales</em>, <em>Mononegavirales</em>, <em>Virgaviridae</em>, <em>Iflaviridae</em>, <em>Nodaviridae</em>, <em>Solemoviridae</em>, and <em>Phasmaviridae</em>. Notably, no core virus was consistently present across the studied populations, and field-collected samples revealed a greater diversity of ISVs compared to those from laboratory samples. In addition, we detected plant-infecting viruses and mycoviruses associated with the pest. This study represents the first description of the RNA virome associated with <em>T. absoluta</em>, providing valuable insights into its biological and ecological interactions. It also lays the foundation for future studies aimed to clarify the biological roles of ISVs.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108340"},"PeriodicalIF":3.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and genomic characterization of a cypovirus isolated from golden muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)","authors":"Iyyangar Deepika ,&nbsp;Abhishek Singh ,&nbsp;Indira Kumar ,&nbsp;Gowtham K ,&nbsp;Roja Gnanasekaran ,&nbsp;Himanshu Dubey ,&nbsp;Rajal Debnath ,&nbsp;Pawan Shukla ,&nbsp;Kangayam M. Ponnuvel ,&nbsp;Vikram Kumar ,&nbsp;K.P. Arunkumar ,&nbsp;Hanamant Gadad ,&nbsp;Prashanth Sangannavar ,&nbsp;Kartik Neog ,&nbsp;Vankadara Sivaprasad ,&nbsp;S.Manthira Moorthy ,&nbsp;Rashmi Santhoshkumar ,&nbsp;G. Sivakumar ,&nbsp;Sanjay Ghosh ,&nbsp;H.S. Subramanya ,&nbsp;Gangavarapu Subrahmanyam","doi":"10.1016/j.jip.2025.108343","DOIUrl":"10.1016/j.jip.2025.108343","url":null,"abstract":"<div><div>A potential viral pathogen, cypovirus causing cytoplasmic polyhedrosis in the Indian golden muga silkworm, <em>Antheraea assamensis</em> Helfer, was isolated and characterized. Electron microscopy analysis confirmed the presence of typical occlusion bodies with icosahedral virions embedded in polyhedral matrices. The viral genome was dsRNA totaling approximately 24.9 kb in size, divided into 10 discrete segments containing one single open reading frame (ORF) each. Genomic analysis revealed conserved non-coding sequences such as ‘AGUAAU’ and ‘AUAGAGC’ at 5′ and 3′ termini, respectively. Functional annotation identified the presence of structural proteins, such as major and minor capsid proteins, as well as enzymatic proteins, including RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis using deduced amino acid sequences of viral RdRp gene indicated a close evolutionary relationship between the cypovirus isolated from <em>Antheraea assamensis</em> and Antheraea mylitta cypovirus 4 (AmCPV4), thus we named the Indian golden muga silkworm cypovirus as Antheraea assamensis cypovirus 4 (AaCPV4). Nevertheless, AaCPV4 does not have a genomic segment 11 which was earlier reported in AmCPV4. Bioassay studies demonstrated high virulence, with 100 % larval mortality observed within 12 days of infection due to cytoplasmic polyhedrosis. Tissue tropism studies revealed significant viral replication in the midgut, hemolymph, and malpighian tubules, with the midgut exhibiting the highest viral load (9.65 log copy number of RdRp gene/µg of RNA). Cross-transmission experiments demonstrated AaCPV4 host specificity, infecting only in <em>Antheraea</em> species (<em>A. mylitta</em> and <em>A. assamensis</em>), while other lepidopteran insects including silkworms <em>Bombyx mori</em>, and <em>Samia ricini</em> were not susceptible to AaCPV4 infection. According to morphological, genetic, and biological characteristics, we suggest that AaCPV4 is an isolate of the species <em>Cypovirus antheraeae</em> (family <em>Spinareoviridae</em>).</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108343"},"PeriodicalIF":3.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA metagenomics revealed insights into the viromes of honey bees (Apis mellifera) and Varroa mites (Varroa destructor) in Taiwan","authors":"Fang-Min Chang ,&nbsp;Yen-Hou Chen ,&nbsp;Pei-Shou Hsu ,&nbsp;Tzu-Hsien Wu ,&nbsp;I-Hsin Sung ,&nbsp;Ming-Cheng Wu ,&nbsp;Yu-Shin Nai","doi":"10.1016/j.jip.2025.108341","DOIUrl":"10.1016/j.jip.2025.108341","url":null,"abstract":"<div><div>The honey bee (<em>Apis mellifera</em>) is a vital pollinator for crops. However, they are infested by an ecto-parasite that has spread worldwide, <em>Varroa</em> mite (<em>Varroa destructor</em>). The <em>Varroa</em> mite is a vector of various western honey bee viruses. In this study, the prevalence of seven honey bee viruses (Deformed wing virus, Lake Sinai virus, Acute bee paralysis virus, Sacbrood virus, Kashmir bee virus, Black queen cell virus, Israeli acute paralysis virus), was screened with the honey bees, which were collected from fourteen apiaries from March 2023 to January 2024, and the <em>Varroa</em> mites, which were collected from two apiaries from July to October 2023 by using RT-PCR. Subsequently, metagenomic analyses were conducted on seven honey bee samples and two <em>Varroa</em> mite samples using next-generation sequencing with poly-A capture and rRNA depletion library construction methods. The results showed that 50% to 85.7% of honey bee viruses in each sample were detected by both methods, with up to three additional viruses identified when combining the two approaches. These findings underscore the importance of integrating both methods for comprehensive virome analysis. According to the virome analysis, 28 honey bee viruses were identified in honey bees and 11 in <em>Varroa</em> mites. Among these, 23 viruses were newly recorded in Taiwanese honey bee populations. Notably, three of the newly recorded viruses, Acute bee paralysis virus, Israeli acute paralysis virus, and Apis mellifera filamentous virus, are known to cause symptoms in honey bees, posing potential risks to their health. Six of these viruses were also detected in <em>Varroa</em> mites, highlighting their role in viral transmission. This study represents the first virome analysis of honey bees and <em>Varroa</em> mites in Taiwan, providing critical insights into honey bee health and establishing a foundation for future health assessment indices and mitigation strategies.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108341"},"PeriodicalIF":3.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted knockdown of in vitro candidates does not alter Wolbachia density in vivo","authors":"Kimberley R. Dainty ,&nbsp;Johanna M. Duyvestyn ,&nbsp;Heather A. Flores","doi":"10.1016/j.jip.2025.108346","DOIUrl":"10.1016/j.jip.2025.108346","url":null,"abstract":"<div><div>The bacterial endosymbiont <em>Wolbachia</em> has emerged as an effective biocontrol method to reduce arbovirus transmission. Transinfection of <em>w</em>Mel <em>Wolbachia</em> from <em>Drosophila melanogaster</em> to <em>Aedes aegypti</em> results in the transfer of important <em>Wolbachia</em>-induced phenotypes including the reproductive modification, cytoplasmic incompatibility, and inhibition of viruses including dengue and chikungunya. However, the mechanisms underlying these critical traits as well other <em>Wolbachia</em>-host interactions are still not fully understood. Recently an <em>in vitro</em> genome wide RNAi screen was performed on <em>w</em>Mel-infected Drosophila S2 cells and identified large cohorts of host genes that alter <em>w</em>Mel density when targeted. If these findings can be replicated <em>in vivo</em>, this would provide a powerful tool for modulating <em>w</em>Mel density both systemically and in a tissue-specific manner allowing for interrogation of <em>w</em>Mel-host interactions. Here, we used the GAL4/UAS system to express RNAi molecules targeting host gene candidates previously identified to dysregulate <em>w</em>Mel density <em>in vitro</em>. We found systemic knockdown of two candidate <em>D. melanogaster</em> genes does not lead to <em>w</em>Mel density dysregulation. To explore the lack of consistency between our study and previous work, we also examined native tissue-specific density of <em>w</em>Mel in <em>D. melanogaster</em>. We show density is varied between tissues and find that individual tissue densities are not reliable linear predictors of other tissue densities. Our results demonstrate the complexities of implementing <em>in vitro</em> findings in systemic applications.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108346"},"PeriodicalIF":3.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of calreticulin in the antiviral response of Litopenaeus vannamei against white spot syndrome virus","authors":"Siriluk Maskaew ,&nbsp;Methaporn Meethong ,&nbsp;Panumas Puechpon ,&nbsp;Benjaporn Phupet ,&nbsp;Naeem Madsari ,&nbsp;Sumalee Obchoei ,&nbsp;Prapaporn Utarabhand ,&nbsp;Phanthipha Runsaeng","doi":"10.1016/j.jip.2025.108336","DOIUrl":"10.1016/j.jip.2025.108336","url":null,"abstract":"<div><div>Calreticulin (CRT) is a highly conserved calcium-binding protein that participates in various biological processes such as calcium homeostasis, molecular chaperoning, and immune function. In the present study, the complete cDNA sequence of CRT (<em>Lv</em>CRT) was cloned and characterized from <em>Litopenaeus vannamei</em>. The deduced amino acid sequence of <em>Lv</em>CRT contained two conserved CRT family signatures, three conserved repeated CRT family motifs, and an HDEL motif, a signature sequence for endoplasmic reticulum retention. The highest mRNA expression of <em>Lv</em>CRT was detected in the hepatopancreas of healthy shrimp. When the shrimp were challenged with white spot syndrome virus (WSSV), significantly upregulated expression of LvCRT was detected at 24 h post-injection (hpi). The recombinant protein of LvCRT (rLvCRT) was produced, and its functions were characterized. It could induce agglutination of all tested microorganisms, especially <em>Vibrio parahaemolyticus</em>, which could be inhibited by lipopolysaccharide (LPS) and <em>N</em>-acetyl neuraminic acid (NeuNAc). rLvCRT could bind not only to saccharides on microbial surfaces but also to the recombinant protein of C-type lectin (CTL) containing a low-density lipoprotein receptor domain from <em>Litopenaeus vannamei</em> (rLvLdlr). The rLvCRT-rLvLdlr complex could interact with a recombinant protein of WSSV (rVP28), impairing WSSV infection. These findings confirm that LvCRT plays a role in shrimp immunity. LvCRT might cooperate with other CTLs in antiviral activity by binding to WSSV to prevent the penetration of WSSV into the host cells.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108336"},"PeriodicalIF":3.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection with infectious precocious virus (IPV) suppresses infection with Decapoda iridescent virus 1 (DIV1) in Macrobrachium rosenbergii","authors":"Haoyu Lou ,&nbsp;Guohao Wang ,&nbsp;Xuan Li ,&nbsp;Chengyan Zhou ,&nbsp;Xiaomeng Guo ,&nbsp;Liying Wang ,&nbsp;Qiongying Tang ,&nbsp;Guoliang Yang ,&nbsp;Xuan Dong ,&nbsp;Jie Huang","doi":"10.1016/j.jip.2025.108337","DOIUrl":"10.1016/j.jip.2025.108337","url":null,"abstract":"<div><div>In recent years, diseases caused by infectious precocious virus (IPV) or Decapoda iridovirus 1 (DIV1) have resulted in significant losses to the giant freshwater prawn (<em>Macrobrachium rosenbergii</em>) industry, threatening sustainable aquaculture development of this species. Co-infection with these two viruses was found in farmed prawns. This study investigated the biological characteristics of co-infection in <em>M. rosenbergii</em> through artificial infection with IPV and DIV1. The clinical signs of prawns co-infected with both viruses were observed, histopathological analysis, RT-qPCR or qPCR and <em>in situ</em> hybridization or <em>in situ</em> DIG-labeling-loop-mediated DNA amplification experiments to confirm the co-infection in prawns. The results showed that infection with DIV1 in <em>M. rosenbergii</em> was suppressed by infection with IPV in the co-infection group with a significant lower mortality, weaker histopathological changes, and reduced DIV1 loading compared with prawns only infected with DIV1. This study offers valuable insights for the prevention and control of these dual diseases.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108337"},"PeriodicalIF":3.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of Dorsal switch protein 1 Effect on Growth, Development, and survivability of Tenebrio molitor (Coleoptera: Tenebrionidae)","authors":"Md. Mahi Imam Mollah","doi":"10.1016/j.jip.2025.108338","DOIUrl":"10.1016/j.jip.2025.108338","url":null,"abstract":"<div><div>Dorsal switch protein 1 (DSP1) of insects and high mobility group box 1 (HMGB1) protein of vertebrates are homologous. Both HMGB1 and DSP1 act as damage-associated molecular pattern (DAMP) molecules. Previous studies reported that DSP1 plays a DAMP role in mealworm, <em>Tenebrio molitor,</em> by activating immune genes like antimicrobial peptides (AMPs), phospholipase A₂ (PLA₂), phenoloxidase (PO) and regulating nodulation. However, RNAi of <em>Tm-DSP1</em> suppresses these genes and their immune responses. While the immune role of DSP1 is established, its function in growth, development and survivability of <em>T. molitor</em> larvae is unknown. This study was designed to assess the role of DSP1 in the above-mentioned aspects. <em>Tm-DSP1</em> gene was expressed in all the developmental stages and tissues of <em>T. molitor</em> larvae. The highest expression was observed in L6 larval stage and fat body tissue. <em>Tm-DSP1</em> detection by western blotting supports its expression in <em>T. molitor</em> samples. Following bacterial challenge, DSP1 expression was upregulated in immune-related tissues, including hemocyte, fat body, and midgut. Detection of increased DSP1 in bacteria-challenged plasma ensures the release of DSP1 in plasma from the cell nucleus of immune-challenged larvae. Additionally, RNAi knockdown of <em>Tm-DSP1</em> led to reduced larval growth (body length) and development (body weight) of <em>T. molitor</em> larvae resulting in increased mortality of the larvae. These findings suggest that DSP1 regulates the growth, development, and survival of <em>T. molitor</em>. However, whether DSP1 directly plays a role in these physiological processes or whether it interrupts any other genes to achieve these effects is still unknown. Further study is required to clarify this issue.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108338"},"PeriodicalIF":3.6,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of Beauveria bassiana interaction with Nicotiana benthamiana reveals signatures of N. Benthamiana growth promotion and enhanced defense responses","authors":"Yunxia Liu ,&nbsp;Xin Zhao ,&nbsp;Yongxiong Fan ,&nbsp;Pengxia Huo ,&nbsp;Shuaishuai Huang ,&nbsp;Huifang Wang ,&nbsp;Zhuoyue Lu ,&nbsp;Zhibing Luo ,&nbsp;Yongjun Zhang","doi":"10.1016/j.jip.2025.108334","DOIUrl":"10.1016/j.jip.2025.108334","url":null,"abstract":"<div><div>Many entomopathogenic fungi form intimate (<em>epi</em>- and <em>endo</em>-phytic) associations with that plant that can stimulate plant growth and /or improve resistance to pathogens and insect pests. However, little is known concerning global gene networks that mediate such responses. <em>Nicotiana benthamiana</em> seedlings were artificially colonized by the entomogenous fungus, <em>Beauveria bassiana,</em> and the root tissues were examined via comparative transcriptome analyses performed versus fungal cells grown <em>in vitro</em> on dried root biomass<em>.</em> Plant hormone pathways, and genes involved in photosynthesis, immune defense response, and nutrient metabolism were triggered in roots after fungal colonization. Fungal differentially expressed genes during plant colonization included plant cell wall-degrading enzymes, and those involved in lipid metabolism, detoxification, and fungal cell wall remodeling, the latter suggesting reduction in the exposure of pathogen related molecular patterns to avoid perception by the plant immune system. Fungal metabolic genes involved in amino acid, nitrogen, sulfur and carbohydrate assimilation were activated, nutrient exchange with the plant host. Exchange was confirmed by detection of sulfur in the seedling that was increased by the fungal colonization. A set of fungal secondary metabolism-associated genes were also upregulated during the plant interaction, which might contribute to plant resistance against pathogens or/and insect pest. In addition, <em>B. bassiana</em> expressed a suite of effector/elicitor genes consistent with triggering plant growth and/or immune defense response pathways. These results revealed global gene networks active in both the plants and the fungus as a consequence of their symbiotic interaction, and provides insights into the molecular determinants and physiological responses affected.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108334"},"PeriodicalIF":3.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}