Journal of invertebrate pathology最新文献

{"title":"Ingestion of polystyrene microplastics by Bombyx mori larvae disrupts midgut epithelial barrier integrity and potentially promotes susceptibility to BmNPV infection","authors":"Wenjing Shao ,&nbsp;Yani Kang ,&nbsp;Wenbin Yu ,&nbsp;Yifei Kong ,&nbsp;Yiming Huo ,&nbsp;Zihan He ,&nbsp;Xiaolong Hu ,&nbsp;Xing Zhang","doi":"10.1016/j.jip.2025.108463","DOIUrl":"10.1016/j.jip.2025.108463","url":null,"abstract":"<div><div>Microplastics (MPs) are ubiquitous environmental contaminants with potential implications for host-virus interactions, which has attracted global interest because MPs have been recognized as emerging pollutants. Despite growing concern over MPs, their influence on viral infections, particularly in invertebrate models, remains poorly understood. In this study, we employed the silkworm, <em>Bombyx mori</em>, as a model organism to investigate the impact of microplastic (MP) exposure on viral replication and the gut microbiome. Real-time PCR and Western blotting results showed that exposure to MPs was accompanied by significantly increased BmNPV viral gene expression (<em>ie-1</em>, <em>vp39</em>, <em>gp41</em>) and VP39 production in the midgut, suggesting an increase in BmNPV replication. Exposure to MPs disrupted gut microbiome composition, leading to a decrease in beneficial bacteria (e.g., <em>Vibrionaceae</em>) and an increase in opportunistic pathogens, potentially contributing to increased susceptibility to BmNPV. Notably, the increased permeability of the midgut epithelium in response to MP exposure likely facilitated viral entry and enhanced infection. These findings underscore the potential risks of MPs in facilitating viral replication and systemic infection and emphasize the necessity of further research to explore the complex relationships between MPs, gut microbiota, and viral infections across different ecological settings.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108463"},"PeriodicalIF":2.4,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When a mosquito ingests blood, the phagocytic activity of hemocytes increases with warmer environmental temperature and aging","authors":"Jordyn S. Barr,&nbsp;Saksham R. Saksena,&nbsp;Abby Callahan-Muller,&nbsp;Edith Simpson,&nbsp;Julián F. Hillyer","doi":"10.1016/j.jip.2025.108459","DOIUrl":"10.1016/j.jip.2025.108459","url":null,"abstract":"<div><div>Most female mosquitoes feed on blood to gain the nutrients needed for oogenesis, but the process of blood feeding often exposes mosquitoes to pathogens. Blood feeding and infection both activate the hemocytes that drive cellular immune responses, and the efficacy of immune responses like phagocytosis varies with environmental temperature, aging, and their interaction. Here, we quantified the hemocyte-mediated phagocytosis response in the hemocoel of adult female mosquitoes during the digestion of a blood meal and thereafter, focusing on mosquitoes that had been reared at 27 °C, 30 °C or 32 °C, and were 3, 5 or 10 days old at the time of blood feeding. We discovered that, in blood-fed mosquitoes, phagocytic activity generally increases when the temperature is warmer and when the mosquito is older. Moreover, within the first three days after a blood meal, warmer temperature does not accelerate the senescence of the phagocytosis response. Altogether, these findings demonstrate that ingesting blood changes how warmer temperature, aging, and their interaction alters the phagocytic response of hemocytes, which has implications for how mosquitoes respond to infection and survive in their environment.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108459"},"PeriodicalIF":2.4,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, replication, and tissue-specific localization of Euschistus heros virus (EhV) across life stages of the Neotropical brown stinkbug Euschistus heros (Hemiptera: Pentatomidae)","authors":"Ethiane Rozo Santos ,&nbsp;Raul Alberto Laumann ,&nbsp;Bergmann Morais Ribeiro ,&nbsp;Andreza F. de Bem ,&nbsp;Daniel M.P. Ardisson-Araújo","doi":"10.1016/j.jip.2025.108457","DOIUrl":"10.1016/j.jip.2025.108457","url":null,"abstract":"<div><div>The Neotropical brown stink bug,<!--> <em>Euschistus heros</em> <!-->(Hemiptera: Pentatomidae), is a major agricultural pest in Brazilian soybean crops, exhibiting increasing insecticide resistance in field. This study investigates the dynamics of<!--> <!-->Euschistus heros<!--> <!-->virus (EhV), a multispecies iflavirus, in laboratory populations of<!--> <em>E. heros</em>, revealing a persistent, systemic virus infection across all life stages. Screening of 99 adults showed 100 % EhV genome prevalence in mated individuals, while virgin males and females had an 80 % infection rate. Viral loads peaked in freshly laid eggs (590,462 ± 27,426 copies/ng RNA), suggesting likely maternal transmission, before declining sharply in nymphs with an 18.08-fold reduction by the fifth instar. Adults exhibited sex- and mating-status-dependent variation, with virgin females harboring the highest copy number (227,380 ± 144,938 copies/ng RNA), which is 2.87-fold higher than mated females. Tissue-specific analysis revealed elevated EhV genome accumulation in legs/wings (221,723 ± 102,120 copies/ng RNA in males) and heads, with significantly lower loads in thoraces and abdomens. Strand-specific RT-qPCR suggested that EhV may exhibit replicative activity across developmental stages, as indicated by approximately balanced levels of genomic and replicative strands. Additionally, transmission electron microscopy of antennal tissues from an infected male individual revealed cytoplasmic icosahedral virions (∼30 nm), providing the first ultrastructural evidence of EhV assembly. These findings suggest that EhV may establish a stable, replicative association with <em>E. heros</em> and point to vertical transmission as a plausible mechanism of persistence. Nonetheless, confirmation of this pattern will require additional analyses with larger sample sizes, field collected insects, and complementary experimental approaches.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108457"},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological investigation and whole genome of Vp AHPND CGVP22 isolated from an acute hepatopancreatic necrosis disease (AHPND) outbreak in Korea","authors":"C.W.R. Gunasekara,&nbsp;L.G.T.G. Rajapaksha,&nbsp;S.H.M.P. Wimalasena,&nbsp;H.N.K.S. Pathirana,&nbsp;Gee-wook Shin","doi":"10.1016/j.jip.2025.108456","DOIUrl":"10.1016/j.jip.2025.108456","url":null,"abstract":"<div><div>AHPN disease, caused by <em>Vibrio parahaemolyticus</em>, poses a significant threat to global shrimp farming. Here, we investigated the epidemiological relationships of the AHPND- causing <em>Vp _AHPND</em> CGVP22, isolated from the 2016 AHPND outbreak in Pyeongtaek, Korea, through whole-genome sequencing (WGS). Initially, the presence of <em>pir^AB</em> genes was confirmed by PCR and nested PCR assays, which produced specific bands of 284 base pairs (bp) for <em>pir^A</em> and 392 bp for <em>pir^B</em>, and a 230 bp fragment in the AP4 nested PCR. The assembled whole-genome sequence of <em>Vp _AHPND</em> CGVP22 comprised of a total size of approximately 5.4 Mbp, consisting of 2 circular chromosomes (3.4 Mbp and 2.0 Mbp) and a 69 kbp plasmid. The RAST genome annotation identified 386 subsystems, including those related to virulence, motility, and iron acquisition. <em>In silico</em> multi-locus sequence typing (MLST) assigned <em>Vp _AHPND</em> CGVP22 to sequence type ST1166, and clustered with other ST1166 strains from Vietnam and China. Comparative genomic analysis revealed that <em>Vp _AHPND</em> CGVP22 exhibited 52 single nucleotide polymorphisms (SNPs) and 99.96 % average nucleotide identity (ANI) relative to the 13028/A3 strain, and 973 SNPs with 99.87 % ANI compared to the 20130629002S01 strain. The plasmid of <em>Vp _AHPND</em> CGVP22 showed 99.94 % nucleotide identity to the pVPA3-1 plasmid of the 13028/A3 strain, suggesting a close phylogenetic relationship. Furthermore, <em>Vp _AHPND</em> CGVP22 and 13028/A3 shared identical prophages (12B12), antibiotic resistance genes, and virulence factors, while other ST1166 strains exhibit distinct profiles. Collectively, these results provide valuable insights into the genomic and evolutionary relationships of <em>Vp _AHPND</em> CGVP22, contributing to a better understanding of the epidemiology of AHPN disease.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108456"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen diversity of the non-native narrow-clawed crayfish (Pontastacus leptodactylus) in a UK water body","authors":"Matthew Harwood ,&nbsp;Josie South ,&nbsp;Alison M. Dunn ,&nbsp;Paul D. Stebbing ,&nbsp;Amy Burgess ,&nbsp;Jamie Bojko","doi":"10.1016/j.jip.2025.108458","DOIUrl":"10.1016/j.jip.2025.108458","url":null,"abstract":"<div><div>Biological invasions are intrinsically linked to introducing associated symbiotic organisms, some of which can be parasitic or pathogenic. The pathogenic risk of an ‘invasive parasite’ (aka. exotic pathogen) stems from its potential to infect native hosts and induce behavioural change or mortality, with the pathogen potentially presenting a greater risk than the host. Conversely, parasites translocated by invasive hosts may also reduce the impact of their host, indirectly curbing the hosts impact on the invaded ecosystem. In this study, we develop a pathogen profile for the narrow-clawed crayfish, <em>Pontastacus leptodactylus</em>. This is a non-native species in the United Kingdom, and poses a possible risk as a sink for invasive parasites. We use histopathology, metagenomics and metratranscriptomics to outline the symbiotic diversity harboured by a <em>P. leptodactylus</em> population from West Yorkshire, England.</div><div>We discovered several protozoan and bacterial species that appear to be putatively commensal with this invader, as well as several RNA viruses (<em>Hepelivirales</em>; <em>Picornavirales</em>; <em>Nodaviridae</em>, and others) that may be more pathogenic in nature. Microsporidia and <em>Nudiviridae</em> were absent in our population sample set, as were all metazoan obligate parasites, such as trematodes and acanthocephalans. Using the novel genomic and pathological data available to us, we have explored the evolutionary history of each symbiotic species and provided an initial assessment on the putative risk to native species.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108458"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of a new species, Cyclopispora nantong n. gen. n. sp. from the adipose tissue of Eucyclops speratus (Crustacea: Cyclopidae)","authors":"Meiqi Weng ,&nbsp;Chuangzheng Yang ,&nbsp;Xunbo Miao ,&nbsp;Nan Sun ,&nbsp;Mei Cao ,&nbsp;Suxin Liu ,&nbsp;Jinyong Zhang ,&nbsp;Xingqiang Wang","doi":"10.1016/j.jip.2025.108454","DOIUrl":"10.1016/j.jip.2025.108454","url":null,"abstract":"<div><div>A new microsporidium, <em>Cyclopispora nantong</em> n. gen. n. sp., was described from the adipose tissue of <em>Eucyclops speratus</em> collected from a eutrophic water body of Nantong city, Jiangsu province, China. Infected copepods generally appeared opaque due to numerous spores accumulated in the adipose tissue. The earliest developmental stages observed were uninucleate meronts which resided in direct contact with the host cell cytoplasm. Mature spores were pyriform, monokaryotic, and measured 4.38 ± 0.20 (4.14–4.81) µm in length and 2.94 ± 0.09 (2.78–3.12) µm in width. The bipartite polaroplast consisted of the wide anterior lamellae and the tightly packed posterior lamellae. Isofilar polar filament coiled 13–16 turns and arranged in 3–4 rows. The 1306 bp SSU sequence obtained from <em>E. speratus</em> showed the highest similarity (85.9 %) to <em>Hazardia milleri</em> (AY09006) among the identified microsporidia. Phylogenetic analysis indicated that the present species clustered with an unidentified <em>B. latissima lata</em>-infecting species with high support values to form an independent branch which positioned the base of the <em>Conglomerata-Gurleya-Agglomerata-Binucleata-Senoma-Pseudoberwaldia</em> lineage. Based on the morphological characters and ultrastructural features, as well as SSU rDNA-inferred phylogenetic relationships, we propose the establishment of a novel genus (<em>Cyclopispora</em> n. gen.) and species (<em>Cyclopispora nantong</em> n. sp.) to contain this parasite.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108454"},"PeriodicalIF":2.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"American lobster (Homarus americanus) immune gene expression during Aerococcus viridans var. homari challenge","authors":"Zohreh Fazelan ,&nbsp;Nicolas Argenta ,&nbsp;Spencer J. Greenwood ,&nbsp;K. Fraser Clark","doi":"10.1016/j.jip.2025.108455","DOIUrl":"10.1016/j.jip.2025.108455","url":null,"abstract":"<div><div>The Canadian lobster (<em>Homarus americanus</em>) fishery is Canada’s most economically significant fishing industry and is a critical component of many rural Atlantic Canadian communities. <em>Aerococcus viridans</em> var<em>. homari</em> is a Gram-positive bacterium that can cause mortalities in wild and live-stocked lobsters. We used an RNA sequencing (RNA-seq) approach to examine the expression of more than 29,000 hepatopancreatic genes in <em>H. americanus</em> during an <em>A. viridans</em> var. <em>homari</em> infection challenge to determine how lobster immune gene expression changes during infection. The infection challenge identified 1,803 differentially expressed genes, of which at least 87 are related to immune or stress responses. Of particular note are several anti-lipopolysaccharide factors (ALFs), pentraxin serum amyloid A (SAA), lectins, Toll-like proteins, cytokines, relish, cactus, myeloid differentiation factor, and genes related to antioxidant defence, coagulation, and hyperglycemic hormone regulation. Compared to a previous study using microarray technology, our RNA-seq approach uncovered a broader array of immune genes that were previously undetected in <em>H. americanus</em>. This study provides additional evidence that ALFs and SAA-5-like proteins are critical players in immune defence. Differential expression of ALFs and lectin genes may indicate a tailored response specific to <em>A. viridans</em> var. <em>homari</em>, highlighting the complexity of the immune system of lobster in recognizing and responding to specific pathogens.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108455"},"PeriodicalIF":2.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Dissecting multitrophic interactions: The relationships among Entomophthora, their dipteran hosts, and associated bacteria\" [J. Invertebr. Pathol. 213 (2025) 108425].","authors":"Zuzanna Płoszka, Karol H Nowak, Marta Tischer, Anna Michalik, Michał R Kolasa, Piotr Łukasik","doi":"10.1016/j.jip.2025.108449","DOIUrl":"https://doi.org/10.1016/j.jip.2025.108449","url":null,"abstract":"","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":" ","pages":"108449"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of helminth infection on sexual morphology, reproduction and survival of the freshwater amphipod, Hyalella patagonica","authors":"Carlos Rauque,&nbsp;Verónica Flores","doi":"10.1016/j.jip.2025.108453","DOIUrl":"10.1016/j.jip.2025.108453","url":null,"abstract":"<div><div>Effects of helminth infection on sexual morphology and reproduction of the crustacean intermediate host are not well understood. Therefore, in the present study, we investigated the effects of parasitic infections on the amphipod <em>Hyalella patagonica.</em> Specimens were collected from Lake Mascardi, Argentina, between spring 2018 and summer 2019. We focused on three parasites: the acanthocephalans <em>Pseudocorynosoma enrietti</em> and <em>Acanthocephalus tumescens</em> and the nematode <em>Hedruris suttonae</em>. Morphological alterations in sexual traits, pairing success, and survival rates were analyzed. Significant morphological alterations were induced by <em>P. enrietti</em>, including castration and mixed sexual characteristics. <em>Hedruris suttonae</em> was the parasite most frequently found in amphipods in precopula, indicating that this parasite does not affect pairing success. Reduced survival rates were found in amphipods infected with <em>H. suttonae</em> or co-infected with <em>P. enrietti</em> and <em>A. tumescens</em>. These findings underscore the varying pathogenic effects of parasites on amphipod hosts and highlight the importance of considering multiple factors when evaluating host-parasite interactions and their ecological consequences in aquatic ecosystems.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108453"},"PeriodicalIF":2.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High levels of Bt cry toxin resistant allele frequency in South Indian populations of cotton pink bollworm, Pectinophora gossypiella Saunders (Gelechiidae: Lepidoptera)","authors":"Suresh R. Jambagi ,&nbsp;M. Mohan ,&nbsp;T. Venkatesan ,&nbsp;K. Muralimohan ,&nbsp;D.N. Kambrekar ,&nbsp;C.P. Mallapur ,&nbsp;Neenu Augustine","doi":"10.1016/j.jip.2025.108452","DOIUrl":"10.1016/j.jip.2025.108452","url":null,"abstract":"<div><div>The lack of compliance with refugia planting by growers of transgenic cotton expressing toxins from <em>Bacillus thuringiensis</em> (<em>Bt</em>) is a major factor contributing to the development of resistance to <em>Bt</em> toxins Cry1Ac and Cry2Ab and outbreak of pink bollworm, <em>Pectinophora gossypiella</em> in several cotton growing regions of India. The present study estimated the resistant alleles frequency in South Indian populations of <em>P. gossypiella</em> against Cry1Ac + Cry2Ab toxins produced by <em>Bt</em> Bollgard II® cotton. Among the 33 established isofemale families, 19 laid fertile eggs and reached the F₁ generation, of which 10 survived the F₂ screen. Further, individuals from four isofemale families survived the reconfirmation test at the F₃ generation, which was conducted to eliminate false-positive lines for the resistance. These four families are the true positives for carrying Cry toxin resistance alleles. The frequency of Cry toxin resistance alleles was estimated at 0.059, indicating a relatively high prevalence of individuals carrying resistant alleles for both Cry1Ac and Cry2Ab toxins. The present study affirms the existence of a high frequency of resistance alleles in individuals of <em>P. gossypiella</em> populations collected from South India. The outbreak-like situation of <em>P. gossypiella</em> in cotton fields underscores the urgent need for developing and implementing alternative technologies within an Integrated Pest Management (IPM) framework. The present estimates provide valuable baseline data for monitoring resistance alleles frequency in pink bollworm populations.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108452"},"PeriodicalIF":2.4,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}