Journal of invertebrate pathology最新文献_第2页

Insights into the Contortylenchus genitalicola (Tylenchida: Allantonematidae) parasitism in Monochamus alternatus (Coleoptera: Cerambycidae) in China 中国外阴孔螨寄生在天牛（鞘翅目：天牛科）中的研究进展。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-31 DOI: 10.1016/j.jip.2026.108561

Lichao Wang , Huan Li , Yajing Song , Junwei Wang , Muhammad Faizan Latif , Chenning Zhang , Ming Xu , Min Li , Fengmao Chen

{"title":"Insights into the Contortylenchus genitalicola (Tylenchida: Allantonematidae) parasitism in Monochamus alternatus (Coleoptera: Cerambycidae) in China","authors":"Lichao Wang , Huan Li , Yajing Song , Junwei Wang , Muhammad Faizan Latif , Chenning Zhang , Ming Xu , Min Li , Fengmao Chen","doi":"10.1016/j.jip.2026.108561","DOIUrl":"10.1016/j.jip.2026.108561","url":null,"abstract":"<div><div><em>Contortylenchus genitalicola</em> is a nematode species belonging to the family Allantonematidae that parasitizes the reproductive organs of <em>Monochamus alternatus</em>. This nematode was first reported in Japan, but the information regarding its occurrence in China remains limited, including parasitic insect species, geographical distribution, and suitable fungi for its reproduction. Here, we determined the nematode taxonomic status by observing its entomoparasitic and mycetophagous morphology and obtained <em>18S</em>, <em>28S,</em> and <em>COI</em> sequences. A total of 11 Cerambycid species were inspected, and nematodes were recovered only in <em>M. alternatus</em> and <em>Spondylis buprestoides</em>, with the former being the primary beetle host. Subsequently, <em>M. alternatus</em> were collected from 12 pine forests across 6 provinces, and it was found that <em>M. alternatus</em> distributed in 10 pine forests in 5 provinces were parasitized by this nematode. Among the eight fungal species screened for nematode propagation, isolates <em>Colletotrichum siamense</em> and <em>Sphaeropsis sapinea</em> are suitable fungi for <em>C. genitalicola</em>. Our findings define the beetle hosts and geographical distribution of <em>C. genitalicola</em> in China and confirm the suitable fungal host for nematode reproduction, thereby providing a solid scientific foundation for exploiting this nematode in biological control targeting <em>M. alternatus.</em></div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108561"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome characterization of Diaphorina citri picorna-like virus from China and a proposal for the new family Psylloidiviridae 中国小柑橘地虱样病毒的全基因组特征及新科木虱病毒科的建立

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-29 DOI: 10.1016/j.jip.2026.108562

Dee Du , Yixuan Kuang , Yuting Jiang , Ying Wang , Mengji Cao , Aijun Huang

{"title":"Complete genome characterization of Diaphorina citri picorna-like virus from China and a proposal for the new family Psylloidiviridae","authors":"Dee Du , Yixuan Kuang , Yuting Jiang , Ying Wang , Mengji Cao , Aijun Huang","doi":"10.1016/j.jip.2026.108562","DOIUrl":"10.1016/j.jip.2026.108562","url":null,"abstract":"<div><div>The Asian citrus psyllid, <em>Diaphorina citri</em>, is the primary vector of ‘<em>Candidatus</em> Liberibacter asiaticus’(CLas), the causal agent of citrus Huanglongbing. Insect viruses are increasingly recognized as important factors influencing vector biology and potential tools for pest management. Here, we report the complete genomes of 13 Chinese isolates of Diaphorina citri picorna-like virus (DcPLV). Genomic characterization revealed an overall picornavirus-like organization but with distinctive features, such as an inverted protein order and a novel IRES. Phylogenetic and pairwise identity analyses placed DcPLV within the order <em>Picornavirales</em>, where it forms a distinct lineage related to, yet deeply divergent from, members of the family <em>Iflaviridae</em>, sharing less than 40% polyprotein identity with all known iflaviruses. Based on this substantial divergence in genome organization, IRES structure, and sequence, we propose the establishment of a new family, “<em>Psylloidiviridae</em>”, and a new genus, “<em>Psylloidivirus</em>”, to accommodate DcPLV and related viruses.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108562"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146080034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and potential application of Thurincin H as an alternative to antibiotics in the control of Streptococcus iniae 苏云金素H作为抗生素替代品控制猪链球菌的特性及潜在应用。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-31 DOI: 10.1016/j.jip.2026.108550

María Zulema Juárez-Cortés , Luz Edith Casados Vázquez , Alma Rosa Rivera Camacho , Marcial Arellano Martínez , Tomas Ortíz Rodríguez , Sergio Francisco Martínez Díaz , César Salvador Cardona-Félix

{"title":"Characterization and potential application of Thurincin H as an alternative to antibiotics in the control of Streptococcus iniae","authors":"María Zulema Juárez-Cortés , Luz Edith Casados Vázquez , Alma Rosa Rivera Camacho , Marcial Arellano Martínez , Tomas Ortíz Rodríguez , Sergio Francisco Martínez Díaz , César Salvador Cardona-Félix","doi":"10.1016/j.jip.2026.108550","DOIUrl":"10.1016/j.jip.2026.108550","url":null,"abstract":"<div><div>In aquaculture, controlling emerging pathogenic bacteria is crucial, making the search for new antimicrobials a priority. We evaluated the antibacterial activity against <em>Streptococcus iniae</em> under <em>in vitro</em> and <em>in vivo</em> conditions of the bacteriocin Thurincin H (ThurH), recombinantly produced in <em>Bacillus thuringiensis</em> genotype Cry-B-pThur and purified via molecular weight screening. The results show that ThurH is stable in seawater and inhibits the growth of <em>S. iniae</em> by 100% at 70 µg/ml, with a MIC of 35 µg/ml. Bactericidal activity was observed within the first 10 min (16 MIC), eliminating 100% of viable cells. The <em>in vivo</em> assay with <em>Artemia franciscana</em> demonstrated that ThurH is non-toxic at 1000 µg/ml. Challenge tests with <em>A. franciscana</em> at 100 mL (4 artemias/mL) and histological analysis demonstrated the efficacy of ThurH in <em>in vivo</em> trials, improving organism survival with two doses (MIC). These results demonstrate for the first time in an <em>in vivo</em> assay that ThurH has potential as an antimicrobial against <em>S. iniae</em> in aquaculture.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108550"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity and physical investigation of a novel fluid bed-dried Bacillus thuringiensis kurstaki formulation 一种新型流床干燥苏云金芽孢杆菌制剂的生物活性和物理性质研究。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-21 DOI: 10.1016/j.jip.2026.108547

Rayan Nasreddine , Gül Ayyildiz , Tayssir El Hammar , Zhanerke Amangeldi , Rim El Jeni , Asime Filiz Caliskan Keçe , Luc Fillaudeau , Dietrich Stephan

{"title":"Bioactivity and physical investigation of a novel fluid bed-dried Bacillus thuringiensis kurstaki formulation","authors":"Rayan Nasreddine , Gül Ayyildiz , Tayssir El Hammar , Zhanerke Amangeldi , Rim El Jeni , Asime Filiz Caliskan Keçe , Luc Fillaudeau , Dietrich Stephan","doi":"10.1016/j.jip.2026.108547","DOIUrl":"10.1016/j.jip.2026.108547","url":null,"abstract":"<div><div>The intensive damage caused by citrus pests such as <em>Phyllocnistis citrella</em> and <em>Prays citri</em> requires the use of efficient, safe, and eco-friendly control measures. This study reports the development, characterization, and evaluation of a novel fluid bed-dried formulation based on <em>Bacillus thuringiensis kurstaki</em> (Btk) strains, namely Lip and Blb1, produced via submerged fermentation on wheat bran. The concentrated pellets using Lip achieved mean values of 9.93 Log<sub>10</sub> CFU/g dry matter (gdm) spores and 117 mg/gdm proteins, with post-formulation of 9.51 Log<sub>10</sub> CFU/gdm and 15 mg/gdm, respectively. Number- and volume-based size distribution functions were analysed for dry formulations (Btk Lip, Btk Blb1, and Delfin®) and solubilized suspensions, demonstrating critical safety and application criteria associated with “fine” and “coarse” populations. For the Delfin® suspension, remaining “coarse” particles (>100 µm: 21.7 %) were specifically reported. Laboratory bioassays against <em>P. citrella</em>, confirmed no significant differences across all formulations, whereas for <em>P. citri</em>, and <em>Spodoptera frugiperda</em> the lethal concentration of 50 % (LC<sub>50</sub>) of Delfin® was significantly lower than Lip- and Blb1 formulations (p < 0.001). In a field trial against <em>P. citrella</em>, Lip and Blb1 induced significantly faster mortality (LT<sub>50</sub> = 1.67 d and 1.61 d, respectively) than Delfin® (LT<sub>50</sub> = 2.89 d, p < 0.001). This study addresses whether fluid bed–dried formulations of locally isolated Btk strains produced on wheat bran–based media can combine physicochemical robustness, effective dispersion, and high insecticidal efficacy against citrus pests, offering a sustainable alternative to commercial Btk products.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108547"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146040954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of 1980s and 1990s Varroa destructor samples from Uruguay provides insights into its spread and increasing virulence 对乌拉圭20世纪80年代和90年代瓦罗亚破坏者样本的分析提供了对其传播和日益增加的毒性的见解。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-17 DOI: 10.1016/j.jip.2026.108535

Paula Parada , Ciro Invernizzi , Juan Pablo Campá , Ivanna Tomasco

引用次数: 0

MrBZIP, a MrWETA-interacting transcription factor, mediates oxidative and heat stress tolerance in conidia of Metarhizium robertsii MrBZIP是mrweta相互作用的转录因子，介导罗伯特绿僵菌分生孢子的氧化和热胁迫耐受

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-29 DOI: 10.1016/j.jip.2026.108558

Hao Wu , Youmin Tong , Cuiming Wang , Yu Luan , Bo Huang

{"title":"MrBZIP, a MrWETA-interacting transcription factor, mediates oxidative and heat stress tolerance in conidia of Metarhizium robertsii","authors":"Hao Wu , Youmin Tong , Cuiming Wang , Yu Luan , Bo Huang","doi":"10.1016/j.jip.2026.108558","DOIUrl":"10.1016/j.jip.2026.108558","url":null,"abstract":"<div><div>Wide application of biopesticides which utilize <em>Metarhizium robertsii</em> conidia as active ingredients is restrained by conidial stress tolerance. Recent research revealed that MrWETA is involved in conidiation, conidial maturation and virulence in <em>M. robertsii</em>. Here, to further study MrWETA functions, we used a yeast two-hybrid system to screen potential MrWETA-interacting proteins in <em>M. robertsii</em>. Through sequencing analysis, a Basic-leucine zipper (bZIP) transcription factor MrBZIP was identified as a potential MrWETA partner. We further discovered that <em>Mrbzip</em> exhibited high activation in conidia and played a vital role in conidial oxidative and heat stress tolerance by regulating stress-related genes. Transcriptomic analysis revealed that deletion of <em>Mrbzip</em> resulted in altered expression of 198 genes, which mostly were involved in cellular component and metabolic pathways in GO and KEGG analyses, respectively. Surprisingly, despite its impact on stress tolerance, loss of <em>Mrbzip</em> did not significantly affect conidiation, conidial germination, or virulence. Our results indicate that MrBZIP, as a potential MrWETA partner, shares functional parallels with MrWETA in governing conidial stress tolerance. This study advances our understanding of stress tolerance regulatory networks in entomopathogenic fungi, which is vital for improving their application under environmental stressors.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108558"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146080037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of gonadal neoplasia in a common garden snail (Cornu aspersum) with intravascular metastasis 一个常见的花园蜗牛性腺肿瘤伴血管内转移1例

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-02-10 DOI: 10.1016/j.jip.2026.108566

Lea A. Mascherrek , Peter Richards-Rios

引用次数: 0

Patagonian king crabs (Lithodes santolla and Paralomis granulosa) and their diseases: Pathogen survey and taxonomic clarification of Areospora rohanae (Microsporidia) 巴塔哥尼亚帝王蟹（Lithodes santolla和paromis granulosa）及其疾病：小孢子虫（Areospora rohanae）的病原调查和分类澄清。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-31 DOI: 10.1016/j.jip.2026.108560

Antonella Frizzera , Nuria Vázquez , Hernán Sacristán , Federico Tapella , Gustavo Lovrich , Amjad Khalaf , Jamie Bojko , Florencia Cremonte

{"title":"Patagonian king crabs (Lithodes santolla and Paralomis granulosa) and their diseases: Pathogen survey and taxonomic clarification of Areospora rohanae (Microsporidia)","authors":"Antonella Frizzera , Nuria Vázquez , Hernán Sacristán , Federico Tapella , Gustavo Lovrich , Amjad Khalaf , Jamie Bojko , Florencia Cremonte","doi":"10.1016/j.jip.2026.108560","DOIUrl":"10.1016/j.jip.2026.108560","url":null,"abstract":"<div><div>Lithodid crabs constitute a valuable natural resource with commercial interest; however, their health status in South America is scarcely studied. The presence of pathogens may have a negative impact on affected populations, endangering the fishery. This study surveys the symbionts present in populations of the king crab, <em>Lithodes santolla,</em> and the stone/false king crab, <em>Paralomis granulosa,</em> using histology, transmission electronic microscopy, and molecular analyses to characterise various symbionts. The histological and molecular data revealed a wide range of infections in <em>L. santolla</em>, including prokaryotic microorganisms, <em>Areospora rohanae</em> (Microsporidia), apicomplexan gregarines, ciliated protozoans, ectoparasitic crustaceans, and various epibiotic associations. In the case of <em>P. granulosa</em>, apicomplexan gregarines, ciliated protozoans, and various ectoparasites were observed. Molecular analyses confirmed the presence of the microsporidian <em>A. rohanae</em>, occurring at high prevalences (30%) in <em>L. santolla</em>, and some bacterial associations. We use metagenomic tools to extract a partial genome of this parasite to aid in its identification and taxonomic position, which leads us to erect the taxonomic orders Astathelohaniida and Areosporida and further clarify the previously assigned: “Glugeida+”.</div><div>The results of this study represent the first broad survey for symbionts in both king crab species and take us another step forward to a more accurate microsporidian taxonomy.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108560"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ascosphaera apis-derived microRNA-like RNAs mediate cross-kingdom regulation of gene expression in western honeybee larvae 蜜蜂源性小rna样rna介导西方蜜蜂幼虫基因表达的跨界调控。

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-23 DOI: 10.1016/j.jip.2026.108534

Xiaoxue Fan , Nian Fan , Tao Wu , Jing Kang , Jiarun Yang , Jing Tian , Xihan Luo , Dafu Chen , Jianfeng Qiu , Rui Guo

{"title":"Ascosphaera apis-derived microRNA-like RNAs mediate cross-kingdom regulation of gene expression in western honeybee larvae","authors":"Xiaoxue Fan , Nian Fan , Tao Wu , Jing Kang , Jiarun Yang , Jing Tian , Xihan Luo , Dafu Chen , Jianfeng Qiu , Rui Guo","doi":"10.1016/j.jip.2026.108534","DOIUrl":"10.1016/j.jip.2026.108534","url":null,"abstract":"<div><div>Chalkbrood fungus <em>Ascosphaera apis</em> (<em>A. apis</em>) produces miRNA-like small RNAs (milRNAs) that may modulate honey bee (<em>Apis mellifera</em>) larval biology through cross-kingdom RNA interference (ckRNAi). We identified 380<strong>,</strong> 106<strong>,</strong> 107, and 110 highly expressed milRNAs (HmilRNAs) in pure spores and infected larval guts at days 4, 5, and 6, respectively. Eighteen HmilRNAs were conserved across all groups, forming a complex regulatory network predicted to target 188 host genes (6,057 mRNAs) associated with critical signaling pathways, including Wnt, Hippo, mTOR, MAPK, and Toll/Imd. Among these, aap-milR-11980-x was validated to directly target the ecdysone-inducible gene <em>E</em>75 and the transcriptional repressor tramtrack (<em>TTK</em>). Dual-luciferase assays confirmed that aap-milR-11980-x represses these targets via specific binding to their 3′-UTR. In vivo modulation of aap-milR-11980-x in 6-day-old larval guts using agomirs and antagomirs successfully altered <em>E</em>75, <em>TTK</em>, and antimicrobial peptide (AMP) transcript levels. However, despite significant molecular regulation, no statistically significant difference in larval survival was observed between treatment groups (<em>P</em> > 0.05). This discrepancy suggests that while aap-milR-11980-x effectively rewires host gene expression, its primary function may be to induce sub-lethal physiological changes, such as developmental delay or immune modulation, rather than acute lethality. These findings indicate that <em>A. apis</em> HmilRNAs fine-tune host cellular responses, potentially to extend the temporal window for fungal development or resource acquisition, highlighting a sophisticated layer of molecular manipulation in the <em>A. apis</em>-honey bee interaction.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"216 ","pages":"Article 108534"},"PeriodicalIF":2.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doxycycline-mediated clearance of Wolbachia is associated with the long-term survival of Diaphorina citri cell lines 多西环素介导的沃尔巴克氏体清除与柑橘地虱细胞系的长期存活有关

IF 2.4 3区生物学

Journal of invertebrate pathology Pub Date : 2026-06-01 Epub Date: 2026-01-13 DOI: 10.1016/j.jip.2026.108532

Ke Wu, Clebson S. Tavares, Bryony C. Bonning

引用次数: 0