Journal of invertebrate pathology最新文献

{"title":"Cytopathology and phylogeny of Pinna nobilis Picornavirus (PnPV) infecting haemocytes of the noble pen shell population from Lake Faro (Southern Italy)","authors":"Carmelo Iaria ,&nbsp;Maria Giovanna Piro ,&nbsp;Sabrina Natale ,&nbsp;Fabiano Capparucci ,&nbsp;Grazia Villari ,&nbsp;Gionata De Vico ,&nbsp;Francesca Carella","doi":"10.1016/j.jip.2025.108381","DOIUrl":"10.1016/j.jip.2025.108381","url":null,"abstract":"<div><div><em>Pinna nobilis</em> Picornavirus (<em>PnPv</em>) is an RNA virus affecting the immune cells of the noble pen shell <em>P. nobilis</em>, which has recently been discovered in two Mediterranean countries, Spain and Italy. As part of the LIFE PINNARCA (LIFE20 NAT/ES/001265) project, a survey was performed to evaluate the health status of the <em>P. nobilis</em> population found in Lake Faro in Sicily (Southern Italy), previously reported as a <em>P. nobilis</em> sanctuary. A conservative sampling consisting of both mantle biopsies and haemolymph was performed on 6 individuals during the survey in 2023. Immune cells were observed at transmission electron microscopy (TEM) and cytology and used for RNA based qPCR. Nucleic acid extraction (DNA and RNA) was performed on the mantle biopsy to detect the presence of the parasite <em>Haplospodium pinnae</em> and the virus <em>PnPv</em> in order to establish its tropism. From TEM, we discovered that the <em>PnPv</em> infection was present in 100 % of the samples. The parasite <em>H. pinnae</em> was detected through PCR in 3 out of 6 animals (50 %); acid fast bacteria were detected in cytological preparations in 2 out of 6 individuals (33 %). Phylogenetic analyses of <em>PnPv</em> supported the novel virus being a member of the <em>Marnaviridae</em> family, likely descending from an algal host ancestor. This discovery provided some important information on evolutionary relationships and taxonomy of picornaviruses, increasing our understanding of the disease outcome and bringing new insight to the cytopathology and phylogeny of <em>PnPV</em>.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108381"},"PeriodicalIF":3.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Perkinsus olseni parasitism and environmental conditions on the gonad maturation and reproductive effort of female Manila clam (Ruditapes philippinarum) on the west and south coasts of Korea","authors":"Thatchaneshkanth Subramaniam ,&nbsp;Hyun-Ki Hong ,&nbsp;Kyung-Il Park ,&nbsp;Young-Ghan Cho ,&nbsp;Kwang-Sik Choi","doi":"10.1016/j.jip.2025.108385","DOIUrl":"10.1016/j.jip.2025.108385","url":null,"abstract":"<div><div>In the intertidal zone, the reproductive dynamics of bivalves are influenced by both biotic and abiotic factors, including spatial and temporal fluctuations in sea surface temperature (SST), food availability, and diseases. Notably, disease proliferation is markedly enhanced under conditions favorable to pathogen entities, such as elevated SST and low food availability. This study examined the associative impacts of <em>Perkinsus olseni</em> parasites on the reproduction of the Manila clam <em>Ruditapes philippinarum</em>, across a latitudinal range covering four sampling sites along the west and south coasts of Korea, ranging approximately 400 km. In late June, we collected adult female clams to assess their condition index, reproductive output, and the prevalence of <em>P. olseni</em> infection. Histology indicated that 94 % of clams from SJ tidal flat, the cooler northwest coast were in the ripe stage. In contrast, clams from WD and GS, the warmer regions of the southwest and south exhibited more progressed stages of gonadal maturation, including the spent and resting phases. Notably, clams at the ripe stage in WD and GS demonstrated significantly lower gonad somatic index (GSI, P &lt; 0.05) than their counterparts in the northwest. Moreover, the intensity of <em>P. olseni</em> infection was substantially higher (P &lt; 0.05) in WD in the south compared to SJ in the northwest. The histopathological index (HPI) revealed minimal tissue damage and lower HPI scores at SJ, in stark contrast to the progressively severe tissue damage and elevated HPI scores observed moving southward along the latitudinal gradient. It was postulated that the combined effects of higher SST, low food availability, and high levels of <em>P. olseni</em> infection are key factors contributing to the reduced GSI and condition index (CI) in ripe clams in WD on the warm southern coast. Additionally, the lower CI observed in clams from WD potentially heightens their vulnerability to diseases by weakening their immune defenses during critical reproductive phases, such as spawning and post-spawning.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108385"},"PeriodicalIF":3.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota induces host midgut immune response and contributes to defending against Bt Cry1Ac protoxin in Ostrinia furnacalis","authors":"Shuzhong Li,&nbsp;Zitian Wang,&nbsp;Fuyun Li,&nbsp;Ling Fang,&nbsp;Zhaoyang Han,&nbsp;Honglun Bi,&nbsp;Congjing Feng","doi":"10.1016/j.jip.2025.108380","DOIUrl":"10.1016/j.jip.2025.108380","url":null,"abstract":"<div><div>Insects’ gut microbiota plays essential roles in the development, digestion, immunity, and pathogen resistance of the host. <em>Bacillus thuringiensis</em> <!-->(Bt)<!--> <!-->is recognized for its ability to generate insecticidal protoxins to kill insect pests. However, systematic studies on the interplay between Bt toxins, host insect gut microbiota, and gut immunity are still lacking for <em>Ostrinia furnacalis</em>. Our findings demonstrate that the Bt Cry1Ac protoxin significantly induces the midgut immune responses, causes a dynamic shift in bacterial communities, and increases the total bacterial load within the midgut and hemocoel of <em>O. furnacalis</em>. In addition, the absence of the gut microbiota significantly increased the susceptibility of <em>O. furnacalis</em> larvae to Cry1Ac protoxin. The reintroduction of five intestinal commensal bacterial strains, OfG3 (<em>Enterobacter cancerogenus</em>), OfG4 (<em>Kluyvera sichuanensis</em>), OfG5 (<em>Klebsiella variicola</em>), OfG6 (<em>Klebsiella grimontii</em>), and OfG7 (<em>Citrobacter freundii</em>), markedly reduced the susceptibility of both axenic and normal <em>O. furnacalis</em> larvae to Bt Cry1Ac protoxin. Our study further demonstrated that the loss of the gut microbiota resulted in a significant down-regulated expression of most immune-related genes, and the introduction of five gut commensal bacteria strains (OfG3-OfG7) significantly up-regulated the expression levels of antimicrobial peptide (AMP) genes in the midgut of normal <em>O. furnacalis</em> larvae. These results suggest a critical role for the gut microbiota in modulating host midgut immunity and mitigating the toxicity of Bt Cry1Ac protoxin in <em>O. furnacalis</em>, offering valuable insights for improving Bt resistance management and developing novel biological control strategies for lepidopteran pests.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108380"},"PeriodicalIF":3.6,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia’s north coast","authors":"Jahnier A. Caicedo ,&nbsp;Gersson M. Vásquez ,&nbsp;Claudia P. Calderón ,&nbsp;Eliana Celis ,&nbsp;Jennifer-Natalie Castro ,&nbsp;Joao Araujo-Junior ,&nbsp;Hung N. Mai ,&nbsp;Arun K. Dhar","doi":"10.1016/j.jip.2025.108382","DOIUrl":"10.1016/j.jip.2025.108382","url":null,"abstract":"<div><div>This study describes the diagnosis and reporting of IHHNV infection in two shrimp farms on Colombia’s north coast in 2021. IHHNV was first detected in frozen <em>Penaeus vannamei</em> samples for export. Epidemiological investigation revealed IHHNV prevalence of 97 % and 36 % in the two farms using WOAH-recommended PCR. Histopathology showed no inclusion bodies, but in situ hybridization confirmed infection. Phylogenetic analysis identified the Colombian IHHNV isolates as genotype II, similar to strains from Ecuador, Brazil, and the US. These findings emphasize the importance of integrating molecular diagnostics, genome sequencing, and histopathology with in situ hybridization for comprehensive IHHNV detection and characterization.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108382"},"PeriodicalIF":3.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-8 modulates immune defense and growth in Arma chinensis Fallou: Implications for pathogen resistance and biocontrol optimization","authors":"Ximei Yuan,&nbsp;Yiqing He,&nbsp;Chen Wang,&nbsp;Yuejun Fu","doi":"10.1016/j.jip.2025.108384","DOIUrl":"10.1016/j.jip.2025.108384","url":null,"abstract":"<div><div><em>Arma chinensis</em> Fallou is a critical biocontrol agent for agricultural pests; nevertheless, its effectiveness is limited by susceptibility to pathogens. The development of artificial diets is anticipated to address this challenge, necessitating a systematic investigation into the physiological and molecular regulatory mechanisms underlying immunity and developmental processes in <em>A. chinensis</em>. This approach further requires the identification of genes and regulatory molecules critical for maintaining immune homeostasis and developmental progression in this species. Using RNAi technology, paraffin sectioning, HE staining, and metabolomic sequencing, the regulatory mechanisms of miR-8 on the immunity and growth of the natural enemy insect <em>A. chinensis</em> were revealed at multiple levels, including individual, cellular, molecular, and metabolic levels. <em>A. chinensis</em> resistance to viral infection by activating innate immunity pathways while enhancing antimicrobial peptide <em>Ach</em>Defensin production. Meanwhile, host miR-8 can inhibit the proliferation of AcMNPV. Metabolomic profiling revealed miR-8′s systemic influence on bile acid biosynthesis, actin cytoskeleton dynamics, and insulin secretion, which coordinate nutrient utilisation and growth <em>via</em> mTOR and IIS signalling. Overexpression of miR-8 significantly increased host body size, shortened developmental time, and promoted fat body expansion. This study reveals miR-8 as a pivotal regulator of <em>A. chinensis</em> immunity and development, presenting innovative options to improve the cost-effectiveness of biological control. These findings enhance the comprehension of miRNA-mediated pathogen resistance in beneficial insects, with ramifications for sustainable pest management and invertebrate disease control.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108384"},"PeriodicalIF":3.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology, proteome and metabolome characterization of the sporoplasm of Ameson portunus (Microsporidia)","authors":"Ya-Li Xu,&nbsp;Min Zhou,&nbsp;Zhi-Qiang Zhu,&nbsp;Zhao-Zhe Xin,&nbsp;Jin-Yong Zhang","doi":"10.1016/j.jip.2025.108383","DOIUrl":"10.1016/j.jip.2025.108383","url":null,"abstract":"<div><div>Infective sporoplasms are the first stage of intracellular survival and propagation for Microsporidia, yet there is a paucity of knowledge regarding their biological characteristics. In this study, we successfully germinated <em>Ameson portunus in vitro</em>, and for the first time, we successfully isolated and purified the sporoplasm of this aquatic microsporidian. Morphological observations show that sporoplasms are spherical, non-refractive, and are similar in size to mature spores. Further ultrastructural observations show that sporoplasms are enclosed by a single membrane, with a single nucleus, their cytoplasm covering a monokaryotic nucleus is relatively granular and homogeneous. Comparative proteomic and metabolomic analyses of sporoplasms and mature spores suggest that 214 significantly differentially expressed proteins (DEPs) and 198 significantly differentially expressed metabolites (DEMs) are possibly involved in the spore germination processes. Several DEPs involved in glycolysis, the pentose phosphate pathway, aminoacyl-tRNA synthesis, nucleotide metabolism and ribosome pathways were upregulated in sporoplasms. Specially, the expression of several transporter proteins was also significantly increased in sporoplasms. Correspondingly, some DEMs involved in aminoacyl-tRNA synthesis, nucleotide metabolism, and amino acid biosynthesis pathways were downregulated. Collectively, our results indicate that significant metabolic activities, including energy and nucletotide and protein synthesis have been initiated in the <em>in vitro</em> produced sporoplasms of <em>A. portunus</em> to prepare for the transition of the parasites from the dormant stage to the active infective stage.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108383"},"PeriodicalIF":3.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses reveal Beauveria bassiana endophyte induced disruption of aphid physiology","authors":"Cuenca-Medina María ,&nbsp;Die José V ,&nbsp;Pérez-Rial Adrián ,&nbsp;Quesada-Moraga Enrique ,&nbsp;González-Mas Natalia","doi":"10.1016/j.jip.2025.108377","DOIUrl":"10.1016/j.jip.2025.108377","url":null,"abstract":"<div><div>Some strains of entomopathogenic fungi are known to colonize crops endophytically, providing systemic protection against insect pests. This colonization has been revealed to reduce aphid survival and reproductive potential. Understanding the molecular mechanisms underlying these effects could provide insight into what occurs to aphids when they feed on endophytically colonized plants. In this study, an RNA sequencing (RNA-Seq) was conducted for transcriptome analyses of the melon aphid <em>Aphis gossypii</em> and its symbiotic bacteria after feeding on <em>Beauveria bassiana</em> endophytically colonized melon plants<em>.</em> The fungal colonization triggers significant alterations in the aphid transcriptome, reflecting molecular responses to fungal propagules and/or plant-induced systemic resistance. Key changes include the up-regulation of genes involved in host plant recognition by the aphid pest, immune system activation, energy production, and defense mechanisms such as toxin adaptation, detoxification, and cell death markers. Genes associated with escape responses, including alarm pheromone production and wing bud differentiation, were also up-regulated. In contrast, down-regulated genes point to disruptions in homeostasis and neuronal function, further impacting aphid physiology and its symbiotic relationship with <em>Buchnera</em>. These molecular perturbations likely underlie the lethal and sublethal effects observed in aphids feeding on <em>B. bassiana</em>-colonized plants. This study identifies critical pathways involved in aphid-fungal-plant interactions, offering valuable insights into the potential of endophytic entomopathogenic fungi for aphid pest management.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108377"},"PeriodicalIF":3.6,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective management of Mud crab Reovirus (MCRV) infection in mud crab, Scylla serrata (Forskal, 1775) culture, using an individual compartmentalized system","authors":"Naveen Nivas S ,&nbsp;Dinesh Kaippilly ,&nbsp;Ayyaru Gopalakrishnan ,&nbsp;Anisha Shafni John ,&nbsp;Savva Sravani ,&nbsp;Saranya Dayalane ,&nbsp;Keerthika Ganesan ,&nbsp;Geeji MT","doi":"10.1016/j.jip.2025.108379","DOIUrl":"10.1016/j.jip.2025.108379","url":null,"abstract":"<div><div>Mud crab (<em>Scylla serrata</em>) aquaculture is a critical contributor to coastal economies and export markets in India. However, the sector faces significant challenges from Mud Crab Reovirus (MCRV), a highly pathogenic virus reported to cause mass mortalities and severe economic losses. This study assessed the performance of different rearing systems—open pen, cage-in-pen, and external cage systems—focusing on their efficacy in managing MCRV transmission, mortality, and production outcomes.</div><div>The open pen system exhibited the highest mortality rate (73 ± 4.57 %) and MCRV infection rate (89.04 ± 3.04 %), primarily due to direct contact and cannibalistic behavior among crabs, which facilitated viral transmission. In contrast, the cage-in-pen and external cage systems significantly reduced mortality (18 ± 2.21 % and 19.1 ± 2.88 %, respectively) and MCRV prevalence (64.13 ± 4.35 % and 65.21 ± 3.78 %, respectively) by physically isolating individuals. These systems achieved higher production yields (11.2 ± 1.5 kg and 10.8 ± 1.8 kg) compared to the open pen system (3.5 ± 0.2 kg). Histopathological and transmission electron microscopy (TEM) analyses confirmed MCRV infection, with cytoplasmic basophilic and eosinophilic inclusions observed in the hepatopancreas, gills, and muscle tissues of infected crabs. TEM revealed icosahedral viral particles (∼70 nm) concentrated in the cytoplasm of connective tissue cells, validating previous findings on MCRV replication.</div><div>This study highlights the critical role of individual compartmentalized systems, such as cage culture, in mitigating MCRV transmission and improving mud crab survival and productivity. While cage culture offers an immediate solution, long-term strategies like enhanced disease surveillance, selective breeding programs, and biosecurity measures are essential for sustainable mud crab aquaculture. These findings provide actionable insights for farmers and policymakers to strengthen the resilience of mud crab farming against MCRV outbreaks<strong>.</strong></div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108379"},"PeriodicalIF":3.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial and peroxisomal Lon proteases are functionally divergent in environmental adaptation and pathobiology of the entomopathogenic fungus Beauveria bassiana","authors":"Hao Zhang,&nbsp;Tian-Jing Chen,&nbsp;Xin-Yi Wang,&nbsp;Ming-Guang Feng,&nbsp;Sheng-Hua Ying","doi":"10.1016/j.jip.2025.108378","DOIUrl":"10.1016/j.jip.2025.108378","url":null,"abstract":"<div><div>Lon protease belongs to the AAA‑ATPase family and plays comprehensive physiological roles via maintaining cellular protein homeostasis. This study seeks to explore the functions of Lon protease in biocontrol potential of the filamentous entomopathogenic fungus <em>Beauveria bassiana</em>. <em>B. bassiana</em> contained mitochondrial and peroxisomal Lon proteases (BbLon1 and BbLon2) which were functionally analyzed via gene disruption and complementation. BbLon1 contributed to fungal tolerance to oxidative, thermal, hyperosmotic, and cell wall-perturbing stresses as well as iron deficiency. Both BbLon1 and BbLon2 co-functioned in conidiation, but BbLon2 had a significant role in the hydrophobicity of conidia and mycelia. BbLon1 played more important roles in pathobiology than BbLon2, including penetration through the cuticle, immune evasion, <em>in vivo</em> development, extracellular acidification and mycosis. Current findings indicate that mitochondrial Lon proteinase plays more predominant roles in lifecycle of <em>B. bassiana</em> than peroxisomal one. This study provides in-depth knowledge for organellar functionality essential for biocontrol efficacy of the entomopathogenic fungi.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108378"},"PeriodicalIF":3.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, identification, pathogenicity and development of a rapid detection technique of Pseudomonas aeruginosa strain GX2021 causing bacterial shell erosion disease in farmed Bellamya aeruginosa","authors":"Cancan Zhang ,&nbsp;Ruixue Shi ,&nbsp;Zhendong Yuan ,&nbsp;Dandan Zhang ,&nbsp;Zhiqiang Wang ,&nbsp;Fuguang Luo ,&nbsp;Yasmeen Gul ,&nbsp;Yanhong Wen ,&nbsp;Yanhe Li","doi":"10.1016/j.jip.2025.108375","DOIUrl":"10.1016/j.jip.2025.108375","url":null,"abstract":"<div><div><em>Bellamya aeruginosa</em> is an economically important freshwater gastropod. Recently, a bacterial shell erosion disease has frequently occurred in <em>B. aeruginosa</em> farms, and has become an important factor threatening the development of <em>B. aeruginosa</em> aquaculture. To explore the cause of disease, we dissected the diseased <em>B. aeruginosa</em> and isolated a dominant strain named <em>Pseudomonas aeruginosa</em> strain GX2021. The pathogenicity of the isolate GX2021 to <em>B. aeruginosa</em> was determined by a direct challenge experiment. Drug sensitivity experiments revealed that <em>P. aeruginosa</em> strain GX2021 was highly sensitive to ceftriaxone sodium, and moderately sensitive to carbenicillin. A PCR assay for rapid detection and identification of <em>P. aeruginosa</em> based on the DNA sequence of the <em>P. aeruginosa</em> strain GX2021 <em>dnaA</em> gene was developed to detect <em>P. aeruginosa</em>. These findings provided a theoretical foundation and practical guidance for prevention and control of shell erosion disease caused by <em>P. aeruginosa</em> in farmed <em>B. aeruginosa</em>.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108375"},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}