Journal of lasers in medical sciences最新文献

{"title":"The Effects of High-Intensity Laser Therapy vs. Low-Level Laser Therapy on Functional Ability and Quadriceps Architecture in Patients with Knee Osteoarthritis: A Single-Blinded Randomized Clinical Trial.","authors":"Kamran Ezzati, Kimia Esmaili, Zoheir Reihanian, Anahita Hasannejad, Mehran Soleymanha, Sara Keshavarz, E-Liisa Laakso, Shahrokh Yosefzadeh Chabok","doi":"10.34172/jlms.2024.66","DOIUrl":"10.34172/jlms.2024.66","url":null,"abstract":"<p><p><b>Introduction:</b> This study aimed to compare the effects of high-intensity laser therapy (HILT) and low-level laser therapy (LLLT) on the disability and architecture of the quadriceps in patients with knee osteoarthritis (OA). <b>Methods:</b> Ninety-eight patients with knee OA (KOA) were selected by convenience sampling and then divided into three groups: control, LLLT and HILT. Disability was determined using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Quadriceps structures including thickness, fascicle length and pennate angle of the vastus lateralis (VL) and thickness, volume and fiber angle of vastus medialis obliquus (VMO) muscles were assessed using ultrasonography. All evaluations were performed before interventions, immediately after interventions, and one month later. Between-group data were analyzed with two-way ANOVA and paired-samples t-test. <b>Results:</b> The within-group comparisons of WOMAC scores before, after and at a one-month follow-up showed significant differences between the groups (<i>P</i><0.001). The VMO thickness revealed significant increases after the treatment in both HILT (<i>P</i><0.001) and LLLT (<i>P</i>=00.03) groups. The between-group comparison revealed a significantly lower score of WOMAC in the HILT group compared to the other groups after a one-month follow-up (<i>P</i>=00.03). VMO thickness showed a significant increase in the HILT group after the treatment (<i>P</i>=0.002). The VL structures and VMO fiber angle and volume did not exhibit significant changes in within-group and between-group comparisons (<i>P</i>>00.05). <b>Conclusion:</b> Both HILT and LLLT may improve functional ability and VMO thickness in patients with knee osteoarthritis. After a one-month follow-up, functional ability was greater in the HILT group.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e66"},"PeriodicalIF":1.4,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diode Laser-Based Photoacoustic Imaging for the Features of Benign and Malignant Uterine Tumors.","authors":"Rima Walhikmah, Nurul Sa'adah, Fikhri Astina Tasmara, Rini Widyaningrum, Hanggoro Tri Rinonce, Mitrayana Mitrayana","doi":"10.34172/jlms.2024.65","DOIUrl":"10.34172/jlms.2024.65","url":null,"abstract":"<p><p><b>Introduction:</b> Photoacoustic imaging (PAI) uses nonionizing radiation for tumor features and thus can be used as an alternative method for medical features without inducing biological effects. The study aimed to characterize a PAI system utilizing a 650-nm diode laser to detect pathological conditions (benign and malignant tumors) in human uterine tissue, including healthy tissue. The benign tumor tissue used is a leiomyoma, which is a benign tumor that occurs in the middle layer of the uterine (myometrium). Meanwhile, the malignant tumor tissue used is a type of endometrial carcinoma, which is a malignant tumor that occurs in the inner layer of the uterine (endometrium). <b>Methods:</b> The human uterine tissue was obtained from the hysterectomy procedure conducted to determine the histopathological diagnosis. The PAI system uses a condenser microphone as the acoustic signal detector and a diode laser as the radiation source. <b>Results:</b> The characterization results of the PAI based on a diode laser with a wavelength of 650 nm and power of 250 mW showed an optimal performance for human uterine tissue in a laser modulation frequency of 16500 Hz and a duty cycle of 50%. Obtained photoacoustic images could differentiate between healthy uterine, leiomyoma, and endometrial carcinoma tissues, as indicated by the intensity level values in each tissue type. Healthy uterine, leiomyoma, and endometrial carcinoma tissues had intensity values of 11.87, 26.84, and 37.26 a.u, respectively. A One-way ANOVA test with Bonferroni post hoc analysis revealed a significant difference in the acoustic intensity level between the groups (<i>P</i><0.05). <b>Conclusion:</b> The resulting intensity levels indicate a direct correlation with the nature of uterine tumors. The poorer the differentiation, the higher the resulting intensity.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e65"},"PeriodicalIF":1.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylene Blue-Mediated Photodynamic Therapy in Combination With Doxorubicin: A Novel Approach in the Treatment of HT-29 Colon Cancer Cells.","authors":"Nima Rastegar-Pouyani, Jaber Zafari, Alireza Nasirpour, Hossein Vazini, Nabaa Najjar, Seyedeh Zohreh Azarshin, Fatemeh Javani Jouni","doi":"10.34172/jlms.2024.64","DOIUrl":"10.34172/jlms.2024.64","url":null,"abstract":"<p><p><b>Introduction:</b> With an alarmingly growing number of patients diagnosed with colorectal cancer, adopting innovative anti-cancer approaches has recently garnered great attention. One interesting concept is the co-administration of cytotoxic agents and safer modalities such as photodynamic therapy (PDT), which can subsequently improve therapeutic efficacy and potentially reduce the risks of severe adverse effects and drug resistance. In the course of PDT, a locally injected photosensitizer (PS) is irradiated with a light source, which subsequently generates reactive oxygen species (ROS) and induces programmed cell death in tumor cells. <b>Methods:</b> In this study, to evaluate the potential anti-cancer effects of chemotherapy combined with PDT, in comparison to each alone, we employed PDT, comprising methylene blue (MB) and diode lasers at 630 and 810 nm wavelengths, in conjunction with the chemotherapeutic agent doxorubicin (DOX). <b>Results:</b> The MTT assay showed that the viability of colorectal cancer HT-29 cells decreased significantly following DOX+PDT treatment. Similarly, lactate dehydrogenase (LDH) release and lipid peroxidation rates were substantially higher in DOX+PDT treatment groups. Lastly, the catalase (CAT) assay indicated that the combination reduced the ability of CAT in the detoxification of H₂ O₂. <b>Conclusion:</b> Our study suggests that MB-mediated PDT combined with chemotherapy might provide a promising avenue to improve therapeutic efficacy and potentially reduce the risk of adverse effects and drug resistance. Without a doubt, further investigations need to delve into the pharmacological advantages and disadvantages of PTD-based combination therapy and optimize its administered doses along with other modalities.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e64"},"PeriodicalIF":1.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CO2 Fractional Laser Induced Skin Micro-Tunnel Thermal Damage Patterns: A Simulation Study.","authors":"Ali Shorakaie, Elaheh Nahvifard, Afshan Shirkavand, Leila Ataie Fashtami, Ezeddin Mohajerani","doi":"10.34172/jlms.2024.63","DOIUrl":"10.34172/jlms.2024.63","url":null,"abstract":"<p><p><b>Introduction:</b> The heat distribution and the resulting thermal damage pattern following the light absorption in tissue can be used for treatment optimization. Besides rejuvenating effects, CO2 fractional-induced microtunnels have recently become a tool for drug delivery. To minimize the unwanted thermal damage in this latter use and to optimize the laser program, we simulated the heat distribution and thermal damage models of CO2 fractional lasers of different sizes, pulse durations, and powers. <b>Methods:</b> COMSOL software is used for simulation. The skin is modeled as three homogeneous layers of epidermis/dermis/hypodermis. The photothermal coefficient of the tissue model and the irradiation laser system (CO2, 10600 nm) are defined as 0.07 mm spot size, 10, 12 and 15 W power range, and 0.5, 10 and 15 ms pulse durations, respectively. <b>Results:</b> Our results show that the power of 10 W with different time pulses creates a better micro-tunnel in the tissue while preventing unwanted injuries. At a power higher than 15 W and 5 pulses, the tissue will be damaged inconsiderably. The fractional laser creates heat only at the desired point of the treatment, and this heat is absorbed through the tissue, and micro-tunnels in it form the tissue. Also, 10 W power with a shorter pulse duration did not have a good effect on the tissue. Instead, by increasing the pulse duration, less damage to the surroundings resulted. <b>Conclusion:</b> Due to the absorbed laser light in tissue and the creation of heat, skin damage as micro-tunnels are caused. The greater distance between the created micro-tunnels indicates better tissue preservation. Also, COMSOL seems to be promising software for preclinical investigations and optimizing laser treatment plans.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e63"},"PeriodicalIF":1.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Low-Level Laser Irradiation of the Red and Infrared Spectral Range for Treating Chronic Testicular Pain: A Randomized Clinical Trial.","authors":"Cyrus Momenzadeh, Mahmoud D Ehghani-Ghorbi, Mohammad Reza Razzaghi, Mohammad Zaki Abbasi, Alireza Jaffari","doi":"10.34172/jlms.2024.62","DOIUrl":"10.34172/jlms.2024.62","url":null,"abstract":"<p><p><b>Introduction:</b> The treatment of chronic testicular pain is a complex condition that will be encountered by most practicing clinicians. In this study, the influence of low-level laser irradiation of the red and infrared spectral range for treating chronic testicular pain was evaluated and compared. <b>Methods:</b> In this double-blind, placebo-controlled randomized clinical trial study, 60 patients were randomly divided into three groups of 20: (1) low-level laser group with red (650 nm, 50 mW), (2) low-level laser group with infrared (820 nm, 100 mW) and (3) laser placebo group. The treatment protocol consisted of 15 minutes, three times a week, for only 12 sessions. Then the patients were evaluated for pain and sexual satisfaction during the follow-up. <b>Results:</b> There was a significant reduction in the pain score in the two groups of the low-level laser group with red (650 nm, 50 mW) and the low-level laser group with Infrared (820 nm, 100 mW) spectra compared with the control, at 6 and 12 weeks after the treatment (<i>P</i><0.05). Also, the level of sexual satisfaction in the two groups of low-power laser with red and infrared spectra showed a significant improvement (<i>P</i><0.05). <b>Conclusion:</b> Low-level laser therapy is a safe, noninvasive, and useful method for treating patients with chronic testicular pain. Therefore, red (650 nm, 50 mW) and Infrared (820 nm, 100 mW) spectra low-level laser therapies improve testicular pain and sexual satisfaction.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e62"},"PeriodicalIF":1.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study of the Use of a 980 nm Diode Laser and 10600nm CO2 in the Treatment of Gingival Melanin Pigmentation.","authors":"Reza Birang, Sayed Mohsen Sadeghi, Ehsan Birang, Nafiseh Birang, Reza Fekrazad, Samaneh Soltani, Nasim Chiniforush, Jaber Yaghini","doi":"10.34172/jlms.2024.61","DOIUrl":"10.34172/jlms.2024.61","url":null,"abstract":"<p><p><b>Introduction:</b> Gingival depigmentation with the help of lasers is known as an effective technique. The aim of this study was to compare the efficiency of 980nm diode and CO2 lasers in the treatment of gingival hyperpigmentation. <b>Methods:</b> In this clinical trial study, 14 individuals (8 females and 6 males) with the age range of 19 to 47 years were selected. One quadrant was randomly treated with CO2 laser (10600 nm, non-contact, super pulse mode) and the opposite quadrant was treated with a diode laser (980 nm, continuous wave [CW], 1.5 W). The area of the pigmented region, Dummett oral pigmentation index (DOPI), the amount of changes in the RGB parameter (red, green, blue) and Δ<i>E</i>* in clinical photographs before, one and three months after the treatment were calculated by ImageJ and Photoshop software. Pain and discomfort were assessed one hour, one day and one week later by the visual analogue scale (VAS). In addition, after 3 months, the cosmetic results achieved for the different therapeutic approaches were evaluated by patients and professional evaluator. Data were analyzed by the paired samples <i>t</i> test, repeated measures ANOVA and Wilcoxon test. <b>Results:</b> The results of this study showed that the duration of CO2 laser treatment was statistically and significantly shorter than the time of 980-diode laser treatment (<i>P</i><0.05). There were no statistically significant differences in the pigmented lesion area (<i>P</i>>0.05), RGB parameter (<i>P</i>>0.05), DOPI index (<i>P</i>>0.05) and ΔE parameter (<i>P</i>>0.05) in one month and three months after treatment follow-ups between the two types of lasers. There was no statistically significant difference in patient satisfaction (<i>P</i>>0.05) and professional evaluation (<i>P</i>>0.05) between the two groups of lasers. Post-treatment pain scores were not statistically significant between the two groups. <b>Conclusion:</b> Both 980-nm diode laser and 10600-nm CO2 are effective in the treatment of gingival depigmentation while CO2 laser radiation with super pulse mode is faster in terms of operating time. While working with the diode laser is more controllable. <b>Trial Registration Number:</b> IRCT20200309046728N1; https://irct.behdasht.gov.ir/.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e61"},"PeriodicalIF":1.4,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful Treatment of Acneiform Rash Induced by Trastuzumab as Neoadjuvant Therapy for Breast Cancer with a 595 nm Pulsed Dye Laser: A Case Report.","authors":"Yunchuan Yang, Yuanxin Li, Jingying Guo, Shuo Sun, Yuangang Lu","doi":"10.34172/jlms.2024.60","DOIUrl":"10.34172/jlms.2024.60","url":null,"abstract":"<p><p><b>Introduction:</b> Trastuzumab is now increasingly being used as a potent HER2 inhibitor in treating breast cancer, while acneiform rashes sometimes arise as skin-related side effects in patients undergoing treatment with HER2 inhibitors, and their specificity as drug-induced eruptions makes their management quite challenging. Pulsed dye laser (PDL) therapy has been utilized to treat a wide variety of vascular lesions, achieving excellent outcomes. <b>Case Report:</b> A 595 nm PDL was used to treat a 38-year-old woman suffering from an acneiform rash induced by trastuzumab as neoadjuvant therapy for breast cancer. One-half of the face received PDL treatment, while the other half served as the control. <b>Conclusion:</b> After just one PDL treatment, the skin lesion demonstrated a remarkable improvement, with a significant reduction in erythematous papules and inflammatory pustules, as well as an improvement in skin thickening. PDL therapy might offer an effective alternative for managing acneiform rashes induced by trastuzumab.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e60"},"PeriodicalIF":1.4,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaginal CO2 Fractional Laser in Women With Vaginal Atrophy: Medical Insights on Short- and Long-term Effects on Genitourinary Symptoms.","authors":"Zinat Ghanbari, Maryam Deldar Pesikhani, Tahereh Eftekhar, Maryam Kazemi, Razieh Akbari, Mahdieh Parsapur, Marjan Ghaemi, Mohadese Dashtkoohi","doi":"10.34172/jlms.2024.59","DOIUrl":"10.34172/jlms.2024.59","url":null,"abstract":"<p><p><b>Introduction:</b> Genitourinary syndrome of menopause (GSM) is a common complication secondary to estrogen depletion which leads to tissue changes in the female genitourinary tract. Here, we sought to investigate the short- and long-term effects of CO₂ laser therapy on symptoms of GSM in postmenopausal women. <b>Methods:</b> In this clinical trial, 47 postmenopausal women with symptoms of GSM were included. Participants underwent vaginal and extra-vaginal CO₂ fractional laser treatment in three sessions, with intervals of one month between each session. Symptom severity, including itching, dyspareunia, vaginal discharge, and dryness, was assessed at each session using a modified Vaginal Health Index (VHI), where the intensity was rated on a Visual Analog Scale (VAS) from 1 to 10 (1 indicating minimal symptoms and 10 representing maximum severity). Additionally, the International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICIQ-UI SF) was administered. Patients were followed for one to three years after the final laser treatment session. <b>Results:</b> The scores of all subscales, including itching, dyspareunia, urinary incontinence, vaginal discharge, and dryness, improved significantly following three sessions of CO₂ fractional laser (<i>P</i> values<0.001). However, except for the urinary incontinence domain (<i>P</i>=0.058), none of the symptoms maintained improved after one to three years from the last intervention. <b>Conclusion:</b> CO₂ fractional laser treatment is appropriate for treating symptoms related to GSM. But it seems that it needs to be used continuously for the maintenance effect on itching, dyspareunia, urinary incontinence, vaginal discharge, and dryness. The exact timing of laser sessions should be identified in further studies since the beneficial outcomes of the intervention seem temporary.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e59"},"PeriodicalIF":1.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of <i>Streptococcus salivarius</i> by Chlorella-Mediated Antimicrobial Photodynamic Therapy.","authors":"Mohammad Reza Karimi, Navid Pourahmad, Shima Afrasiabi, Ardavan Etemadi, Nasim Chiniforush","doi":"10.34172/jlms.2024.58","DOIUrl":"10.34172/jlms.2024.58","url":null,"abstract":"<p><p><b>Introduction:</b> Nowadays, antimicrobial photodynamic therapy (aPDT) has been introduced as one of the minimally invasive methods for disinfection of the surfaces of dental implants. Being derived from seaweed, Chlorella has been used as a photosensitizer in this study. This study aimed to investigate the impacts of aPDT with Chlorella on the rate of reduction of <i>Streptococcus salivarius</i> in vitro. <b>Methods:</b> The minimum inhibitory concentration of Chlorella, the sublethal exposure to 660 nm diode laser irradiation, and the minimum sublethal dose of aPDT utilizing Chlorella against <i>S. salivarius</i> were determined. Finally, the CFU/mL value of each plate was calculated. Then, Tukey HSD and one-way ANOVA tests were utilized for comparison the number of colonies after the interventions. <b>Results:</b> A concentration of 250 µg/mL of Chlorella at an irradiation time of 3 minutes, was identified as a sublethal dose of aPDT for the reduction of <i>S. salivarius</i>. In contrast, the application of aPDT utilizing a 660 nm diode laser for 4 minutes in combination with Chlorella at a final concentration of 500 µg/mL, demonstrated significantly greater efficacy in reducing <i>S. salivarius</i> compared to the other experimental groups (<i>P</i><0.001). <b>Conclusion:</b> Chlorella 500 µg/mL mediated aPDT (660 nm, 4 minutes) has a significant effect on reducing <i>S. salivarius</i> count.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e58"},"PeriodicalIF":1.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a 940 nm Diode Laser and MTAD on the Decontamination of Root Canals With <i>Enterococcus faecalis</i>.","authors":"Alvaro Tapia Gálvez, Abel Teves Cordova, Oniel Elías Juarez Vilcapuma, Katherine Joselyn Atoche-Socola, Sergio Jiménez Sánchez","doi":"10.34172/jlms.2024.57","DOIUrl":"10.34172/jlms.2024.57","url":null,"abstract":"<p><p><b>Introduction:</b> In recent years, various methods such as lasers and different solutions have been investigated to eliminate microorganisms in the root canal. This investigation was aimed at comparing the bactericidal effect on <i>Enterococcus faecalis</i> between a diode laser with a wavelength of 940 nm and a mixture of a tetracycline isomer, an acid, and a detergent (MTAD). <b>Methods:</b> Forty single-rooted human lower premolar teeth were prepared for inoculation with <i>E. faecalis</i> and incubated for a period of 3 weeks. The 40 samples were randomly divided into 4 groups: the 940 nm diode laser group with irradiation through a 200 µm optical fiber with parameters of (1 W/CW,4 cycles, 20s interval); the MTAD group irrigated with 5 mL for 5 minutes; also, a positive group with 3% NaOCl and a negative group with 0.9% saline solution, both irrigated with 5 mL for 5 minutes. For statistical analysis, the Mann-Whitney U and Wilcoxon tests were used. <b>Results:</b> The 3% NaOCl positive control group had greater bacterial reduction after treatment (99.99%) compared to the 940nm diode laser (98.96%) and MTAD (99.34%) groups. Statistically, we found a significant difference between all groups regarding the reduction in the colony count (<i>P</i><0.05). <b>Conclusion:</b> It was found that there was a significant difference in the percentage of reduction for the bacterial colonies between all the groups evaluated, with the greatest antibacterial effectiveness observed with 3% sodium hypochlorite.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e57"},"PeriodicalIF":1.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}